Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| sequences complementary to the brain-specific "identifier" sequences exist in l-type pyruvate kinase mrna (a liver-specific messenger) and in transcripts especially abundant in muscle. | a sequence complementary to the brain-specific identifier sequence has been found in the 3' untranslated extension of the heavy 3.2-kilobase (kb) long liver l-type pyruvate kinase mrna while it is absent in the other two 2- and 2.2-kb long pyruvate kinase mrna species. a 53-base fragment corresponding to this identifier sequence was subcloned in both orientations in the single-stranded bacteriophage m13, both strands being used as probes to detect homologous sequence in different tissues. both s ... | 1986 | 3753703 |
| rapid preparation of bacteriophage dna for sequence analysis in sets of 96 clones, using filtration. | a method is described for the preparation of single-stranded dna from clones in bacteriophage m13 vectors. this procedure allows multiples of 96 clones to be processed at once, utilizing filtration to remove host cells and simplifying the treatment of bacteriophage pellets. the dna produced can be used for sequencing of mutagenesis. | 1986 | 3766942 |
| biosynthesis of reovirus-specified polypeptides. molecular cdna cloning and nucleotide sequence of the reovirus serotype 1 lang strain s3 mrna which encodes the nonstructural rna-binding protein sigma ns. | human reovirus serotype 1 lang strain s3 mrna, which encodes the nonstructural rna-binding polypeptide sigma ns, was cloned as a cdna:mrna heteroduplex in escherichia coli using phage m13. a complete consensus nucleotide sequence was determined. the lang strain s3 mrna is 1198 nucleotides in length and possesses an open reading frame with a coding capacity of 366 amino acids, sufficient to account for a sigma ns polypeptide of 41,179 daltons. comparison of the serotype 1 (lang) s3 sequence with ... | 1986 | 3767989 |
| the region of phage t4 genes 34, 33 and 59: primary structures and organization on the genome. | the product of gene 33 is essential for the regulation of late transcription and gene product 59 is required in recombination, dna repair and replication. the exact functions of both proteins are not known. restriction fragments spanning the genomic area of genes 33 and 59 have been cloned into phage m13 and a 4.9 kb nucleotide sequence has been determined. translation of the dna sequence predicted that gp33 contains 112 amino acids with a mol.wt. of 12.816 kd while gp59 is composed of 217 amino ... | 1986 | 3797242 |
| [isolation of mutant genes for human leukocyte alpha2-interferon by a method of localized mutagenesis]. | an efficient method to obtain the mutant genes for human leucocyte alpha 2-interferon (ifn) has been elaborated. the technique includes the following main stages: cloning of interferon gene in m13mp8 dna; isolation of double-stranded hybrid dna complex, containing ifn gene as a single-stranded fragment; selective modification of a single-stranded hybrid dna by sodium bisulphite; the repair of hybrid dna by dna polymerase i from escherichia coli, transformation of escherichia coli jn103 cells by ... | 1985 | 3842756 |
| cloning, nucleotide sequence, and overexpression of the bacteriophage t4 rega gene. | the bacteriophage t4 rega gene codes for a regulatory protein that controls the expression of a number of t4 early genes, apparently at the level of translation. restriction fragments containing the rega structural gene have been cloned into phage m13, and the nucleotide sequence has been determined. translation of the dna sequence predicted that rega protein contains 122 amino acids, with a mr of 14,620. a dna fragment carrying 85% of the coding sequence of rega has been cloned into the phage l ... | 1985 | 3872458 |
| folding of single-stranded dna on the histone octamer. | a complex between the single-stranded dna of the bacteriophage m13 and the histone octamer was analyzed by electron microscopy, low-angle x-ray diffraction and nuclease analysis. the morphology and the diffraction pattern of the complex strongly resemble those of the nucleosome. these results, as well as the finding of a protected dna fragment about 100 nucleotides long following single-stranded dna specific nuclease digestion, indicate that 'a nucleosome-like' complex can be formed between sing ... | 1985 | 3882454 |
| base-pairing properties of n4-methoxydeoxycytidine 5'-triphosphate during dna synthesis on natural templates, catalyzed by dna polymerase i of escherichia coli. | n4-methoxydeoxycytidine 5'-triphosphate (mo4dctp) was synthesized by reaction of dctp with methoxyamine and then purified by high-performance liquid chromatography (hplc) and used to analyze the specificity of mo4dcmp incorporation during polymerization on natural templates, catalyzed by dna polymerase i of escherichia coli. elongation of synthetic 5'-32p-labeled primers, annealed to single-stranded dna of bacteriophage m13, was carried out in the presence of only three of the four normal dntps; ... | 1985 | 3888268 |
| a new strategy to create ordered deletions for rapid nucleotide sequencing. | a method is described for generating ordered deletions using previously published techniques but a new strategy. this method is simpler than the published ones and has many advantages. target dna is cloned in both orientations into one of the unique restriction enzyme sites adjacent to the complementary region of the commercially available primers in bacteriophage m13. ordered unidirectional deletions are created using bal 31 nuclease and religating into m13 vector dna without the need of purify ... | 1985 | 3891520 |
| membrane protein conformational change dependent on the hydrophobic environment. | two conformational states of the coat protein of the filamentous bacteriophage m13 have been detected in detergent solution by using magnetic resonance techniques. when 3-fluorotyrosine is incorporated in place of the two tyrosine residues in the protein, four 19f nuclear magnetic resonance signals are observed, two for each conformer of the protein. the equilibrium between the two forms can be modulated by ph, temperature, and detergent structure. the rate of interconversion of the isomers is r ... | 1985 | 3893541 |
| sequence-dependent termination of in vitro dna synthesis by cis- and trans-diamminedichloroplatinum (ii). | inhibition of dna replication by the antitumor drug cis-diamminedichloroplatinum (ii) (cis-ddp) has been proposed to be responsible for its cytotoxicity. treatment of primed phage m13 mp8 viral dna templates with the drug followed by second-strand synthesis using large fragment dna polymerase i reveals that cis-ddp forms an adduct with dna that inhibits dna synthesis in vitro. this inhibition occurs at all (dg)n (n greater than or equal to 2) sequences in the template strand, confirming that the ... | 1985 | 3895221 |
| m13 procoat inserts into liposomes in the absence of other membrane proteins. | procoat, the precursor form of the major coat protein of coliphage m13, assembles into the escherichia coli inner membrane and is cleaved to mature coat protein by leader peptidase. this assembly process has previously been reconstituted using lipids and purified leader peptidase in a cell-free protein synthesis reaction (watts, c., silver, p., and wickner, w. (1981) cell 25, 347-353; ohno-iwashita, y., and wickner, w. (1983) j. biol. chem. 258, 1895-1900). we now report that procoat can also cr ... | 1985 | 3902814 |
| partial purification of an enzyme from saccharomyces cerevisiae that cleaves holliday junctions. | an enzyme from saccharomyces cerevisiae that cleaves holliday junctions was partially purified approximately 500- to 1000-fold by deae-cellulose chromatography, gel filtration on sephacryl s300, and chromatography on single-stranded dna-cellulose. the partially purified enzyme did not have any detectable nuclease activity when tested with single-stranded or double-stranded bacteriophage t7 substrate dna and did not have detectable endonuclease activity when tested with bacteriophage m13 viral dn ... | 1985 | 3903750 |
| conserved residues of the leader peptide are essential for cleavage by leader peptidase. | gene 8 of bacteriophage m13 codes for procoat, the precursor of its major coat protein. gene 8 has been cloned into a plasmid and mutagenized. we have isolated mutants of this gene in which procoat is synthesized but is not processed to coat protein. we now describe mutants in the leader region of procoat, at positions -6, -3, and -1 with respect to the leader peptidase cleavage site. these positions are quite conserved among the leader peptides of various pre-proteins. each of these mutant proc ... | 1985 | 3905798 |
| [specific modification of dna at e. coli rna-polymerase binding sites]. | specific modification of promoter regions of dna has been studied. plasmid pk56b1 dna has been used as a model to test rna-polymerase binding with dna under various conditions. rna-polymerase is shown to form specific complexes with dna which are stable in solutions with a moderate ionic strength (0.1-0.2 m nacl), under ph 5-8 in the presence of 0.5 m o-methylhydroxylamine of o-delta-aminooxybutylhydroxylamine. escherichia coli jm103 cells have been transfected with dnas treated with 0.5 m o-met ... | 1985 | 3916215 |
| potential z-dna-forming elements in serum dna from human systemic lupus erythematosus. | dna fragments were isolated from serum of a patient with systemic lupus erythematosus. the majority of the dna was between 150 and 250 base pairs in length. the dna was cloned into phage m13, and 10 recombinants were sequenced. the average gc content of the dna was higher than total human dna (43% against 38%), with some fragments as high as 63%. this dna is rich in alternating purine-pyrimidine segments that are potentially z-dna-forming regions. | 1985 | 3964812 |
| mutational mechanisms by which an inactive replication origin of bacteriophage m13 is turned on are similar to mechanisms of activation of ras proto-oncogenes. | m13 viral strand synthesis is initiated by nicking of the viral strand of the duplex replicative form by the m13 gene ii initiator protein at a specific site within a sequence of about 40 base pairs having dyad symmetry. efficient replication of the m13 viral strand also requires the presence of an adjacent sequence of ca. 100 base pairs. together these sequences constitute the minimal origin for m13 viral strand synthesis. a pbr322 derivative having a 182-base-pair insert of m13 dna contains a ... | 1985 | 3973968 |
| supercoil sequencing: a fast and simple method for sequencing plasmid dna. | a method for obtaining sequence information directly from plasmid dna is presented. the procedure involves the rapid preparation of clean supercoiled plasmid dna from small bacterial cultures, its complete denaturation by alkali, and sequence determination using oligodeoxyribonucleotide-primed enzymatic dna synthesis in the presence of dideoxynucleoside triphosphates. the advantages of the method include speed, simplicity, avoidance of additional cloning steps into single-stranded phage m13 vect ... | 1985 | 3996185 |
| 3-methylcholanthrene-induced expression of the cytochrome p-450c gene. | transcriptional control of 3-methylcholanthrene-dependent cytochrome p-450c nuclear rna induction was directly observed in an in vitro rat liver nuclear transcription system. mercurated and radiolabeled ribonucleotides were incorporated into nuclear rna transcribed in vitro, which was then isolated using thiopropyl-sepharose 6b affinity chromatography. dot hybridization experiments were carried out using bacteriophage m13 subclones of prsa57 (a cdna clone for rat serum albumin), peb339 (a cdna c ... | 1985 | 4004253 |
| high-level expression of m13 gene ii protein from an inducible polycistronic messenger rna. | bacteriophage m13 gene ii has been cloned in the plasmid expression vector ping1 and thereby placed under the control of the inducible arab promoter of salmonella typhimurium. upon induction with arabinose, gene ii is transcribed as part of a polycistronic messenger rna which initiates at the arab promoter. subsequent translation of this message results in the coordinate, high-level expression of several proteins, including the gene ii protein. using this expression system, we have been able to ... | 1985 | 4007491 |
| genes 55, alpha gt, 47 and 46 of bacteriophage t4: the genomic organization as deduced by sequence analysis. | the nucleotide sequence of t4 genes 55, alpha gt, 47 and 46 was determined by a combination of 'classical' procedures and a shotgun approach. small dna fragments generated by frequent cleavage with restriction enzymes or by sonication of restriction fragments were cloned in phage m13 vectors and sequenced by the dideoxy method. the positions of the genes were determined by marker rescue between the corresponding t4 amber mutants and the cloned t4 dna fragments used in the sequencing experiments. ... | 1985 | 4018026 |
| isolation of mutants in m13 coat protein that affect its synthesis, processing, and assembly into phage. | the major coat protein (gene 8 protein) of bacteriophage m13 has been studied intensively as a model of membrane assembly, protein packing, and protein-dna interactions. because this protein is essential for assembly of the phage, very few mutants have been isolated. we have therefore cloned the gene 8 into a plasmid under control of the arab promoter. in the presence of arabinose, the cloned gene is expressed at a rate comparable to that in an m13-infected cell. plasmid-derived procoat is inser ... | 1985 | 4066698 |
| bacteriophage m13 dna-directed in vitro synthesis of gene 5 protein. | 1974 | 4412163 | |
| proceedings: regulation of gene activity in bacteriophage m13 dna. | 1974 | 4461536 | |
| nucleotide sequencing of dna: preliminary characterization of the products of specific cleavages at guanine, cytosine, or adenine residues (bacteriophage m13-ribosubstitution-dna polymerase i-electrophoresis-two-dimensional fingerprinting). | dna synthesized in vitro from a phage m13 template has been cleaved at either guanine, adenine, or cytosine residues by ribosubstitution techniques. fingerprints of the fragments obtained suggest that dna sequencing will be possible with this technique. | 1972 | 4500550 |
| replication of bacteriophage m13: specificity of the escherichia coli dnab function for replication of double-stranded m13 dna. | infection of the temperature-sensitive e. coli mutant hfrh 165/70 (dnab) with the filamentous single-stranded dna phage m13 is abortive at the restrictive temperature. upon infection at 41 degrees , single-stranded phage dna penetrates the cell and is converted in a rifampicin-sensitive step to the double-stranded replicative form (rf). the parental rf attaches to the cell membrane, but subsequent replication of the rf is blocked. it is concluded that in m13 infection semiconservative rf replica ... | 1972 | 4560691 |
| transfer among erwinia spp. and other enterobacteria of antibiotic resistance carried on r factors. | antibiotic resistance carried on r factors was transferred by conjugation from escherichia coli b/r and shigella flexneri 1a to erwinia spp. tetracycline resistance (tetr) carried on r factor r100 drd-56 was transferred from e. coli b/r to strains of erwinia amylovora, e. aroideae, e. atroseptica, e. chrysanthemi, e. cytolytica, e. dissolvens, e. herbicola, e. nigrifluens, and e. nimipressuralis, but not to strains of erwinia carotovora, e. carnegieana, e. dieffenbachiae, e. oleraceae, and e. qu ... | 1972 | 4562410 |
| a coat protein of the bacteriophage m13 virion participates in membrane-oriented synthesis of dna. | several molecules of a protein specified by gene 3 of m13 comprise a minor fraction of the phage coat and have been assigned a role in adsorption to the bacterial cell. we find that the gene-3 protein molecules of the virion are fully conserved in phage that have attached irreversibly to the host cell, and they form a complex with the phage dna when it has been converted to a duplex replicative form. in cells infected at a restrictive temperature with a thermosensitive mutant in gene 3, there is ... | 1973 | 4567335 |
| replication of bacteriophage m13. vii. requirement of the gene 2 protein for the accumulation of a specific rfii species. | 1972 | 4567400 | |
| transmission of lac by the sex factor e in erwinia strains from human clinical sources. | lactose-utilizing (lac(+)) strains of erwinia spp. from human clinical material transfer lac by conjugation to plant strains of erwinia herbicola and erwinia amylovora, to other erwinia strains from human clinical sources, and also to escherichia coli, paracolobactrum arizonae, salmonella typhimurium, and shigella dysenteriae. the frequency of this transfer varies with the donor and recipient strains employed. the lac genes appear stable in these exconjugants, and they are not cured by acridine ... | 1973 | 4582635 |
| synthesis of phage m13 specific proteins in a dna-dependent cell-free system. | 1973 | 4584628 | |
| replication of bacteriophage m13 dna in plasmolysed escherichia coli cells. | 1973 | 4585689 | |
| synthesis of bacteriophage m13-specific proteins in a dna-dependent cell-free system. ii. in vitro synthesis of biologically active gene 5 protein. | it is shown that gene 5 protein of bacteriophage m13 is one of the major proteins synthesized in vitro under the direction of m13 replicative-form dna. by means of dna-cellulose chromatography, this protein has been purified to homogeneity and its biological characteristics have been compared with those of its native counterpart. like native gene 5 protein, the purified, in vitro-synthesized protein binds tightly and selectively to single-stranded, but not to double-stranded, dnas. these results ... | 1973 | 4586780 |
| antiserum inactivation of electrophoretically purified m13 diploid virions: model for the f-specific filamentous bacteriophages. | antiserum inactivation experiments were carried out on electrophoretically purified diploid virions from a cross between two complementing amber mutants of phage m13. the total (homozygous plus heterozygous) diploid population, assayed on a permissive host where only one genome is needed for plaque formation, was inactivated at the same rate as haploids. heterozygous diploids, assayed on a nonpermissive host, where both genomes are needed for plaque formation, were twice as sensitive as haploids ... | 1974 | 4589858 |
| genetic transfer of episomic elements among erwinia species and other enterobacteria: f'lac+. | the episomic element f'lac(+) was transferred, probably by conjugation, from escherichia coli to lac(-) strains of erwinia herbicola, erwinia amylovora, and erwinia chrysanthemi (but not to several other erwinia spp. in preliminary trials). the lac genes in the exconjugants of the erwinia spp. showed varying degrees of stability depending on the strain (stable in e. herbicola strains y46 and y74 and e. amylovora strain ea178, but markedly unstable in e. chrysanthemi strain ec16). the lac genes a ... | 1972 | 4591473 |
| loss of r factors promoted by bacteriophage m13 and the m13 carrier state. | the infection of different hfr strains of escherichia coli bearing derepressed r factors of the fi(+) or fi(-) type can result in the loss of the r factor and the conversion of the infected cells to the r(-) state. this extends earlier observations on the elimination of f' factors by bacteriophage m13 infection. variability in the efficiency of this conversion can arise because of genetic factors independent of the r factor being eliminated. a fraction of the infected but unconverted r(+) cells ... | 1972 | 4591485 |
| complex of bacteriophage m13 single-stranded dna and gene 5 protein. | 1974 | 4594145 | |
| binding, eclipse, and penetration of the filamentous bacteriophage m13 in intact and disrupted cells. | 1974 | 4608378 | |
| replicative intermediates in bacteriophage m13 single stranded dna synthesis. | 1974 | 4611894 | |
| role of bacteriophage m13 gene 2 in viral dna replication. | 1972 | 4648116 | |
| replication of bacteriophage m13. 8. differential effects of rifampicin and nalidixic acid on the synthesis of the two strands of m13 duplex dna. | 1974 | 4817800 | |
| replication of bacteriophage m13. i. sedimentation analysis of crude lysates of m13-infected bacteria. | 1969 | 4890599 | |
| replication of the single-stranded dna bacteriophage m13: messenger rna synthesis directed by m13 replicative form dna. | 1969 | 4902522 | |
| loss of an episomal fertility factor following the multiplication of coliphage m13. | 1969 | 4904513 | |
| replication of the single-stranded dna bacteriophage m13: absence of intracellular phages. | 1970 | 4915300 | |
| interference by bacteriophage t4 in the reproduction of the single-stranded dna phage m13. | 1970 | 4918275 | |
| susceptibility of e. coli k-12 to actinomycin d after infection with phage m13. | 1970 | 4919773 | |
| increased fragility of escherichia coli after infection with bacteriophage m13. | male strains of escherichia coli infected with filamentous phage m13 released the progeny phage particles from intact cells. at the same time, the cells continued to grow and multiply at a slightly lower rate than the uninfected cells. concomitant with the phage release, lipopolysaccharide from the cell wall of the infected cells was also released. the buoyant density of e. coli hfrc in diaginol, 1.25 g/cc, did not change as a result of infection. detergents like sodium dodecyl sulfate and sarko ... | 1970 | 4921123 |
| conformations of the single-stranded dna of bacteriophage m13. | at least two conformations of m13 single-stranded dna have been demonstrated by measuring differences in sedimentation coefficient and by direct visualization in the electron microscope. which form is obtained from infected cells and/or intact phage depends on the ph, ionic strength, and temperature. the slower-sedimenting form can be converted to the faster-sedimenting, single-stranded form by low ionic strength, alkali treatment, formamide, or formaldehyde, but not by exposure to 100 degrees c ... | 1970 | 4922292 |
| replication of bacteriophage m13. iv. synthesis of m13-specific dna in the presence of chloramphenicol. | 1970 | 4923999 | |
| membrane attachment of replicating parental dna molecules of bacteriophage m13. | 1971 | 4927799 | |
| replication of bacteriophage m13. v. single-strand synthesis during m13 infection. | 1971 | 4930572 | |
| non-essentiality of the reca- mutation in the phenomenon of bacteriophage m13-induced elimination of f' factors. | the elimination of f' factors promoted by coliphage m13 infection can occur in reca(+) as well as reca(-) merodiploid strains of escherichia coli k-12. | 1971 | 4934058 |
| genetic control of bacteriophage m13 dna synthesis. | 1968 | 4939035 | |
| replication of bacteriophage m13. vi. attachment of m13 dna to a fast-sedimenting host cell component. | 1971 | 4940969 | |
| a possible role for rna polymerase in the initiation of m13 dna synthesis. | the conversion of single-stranded dna of bacteriophage m13 to the double-stranded replicative form in escherichia coli is blocked by rifampicin, an antibiotic that specifically inhibits the host-cell rna polymerase. chloramphenicol, an inhibitor of protein synthesis, does not block this conversion. the next stage in phage dna replication, multiplication of the doublestranded forms, is also inhibited by rifampicin; chloramphenicol, although inhibitory, has a much smaller effect. an e. coli mutant ... | 1971 | 4941987 |
| replication of bacteriophage m13. detachment of the parental dna from the host membrane and transfer to progeny phages. | 1971 | 4942146 | |
| role of coliphage m13 gene 5 in single-stranded dna production. | 1971 | 4943754 | |
| ultraviolet-induced cross-links in the deoxyribonucleic acid of single-stranded deoxyribonucleic acid viruses as a probe of deoxyribonucleic acid packaging. | deoxyribonucleic acid (dna) from ultraviolet (uv)-irradiated phix174 sediments in alkali at rates up to 1.7 times that of unirradiated phix174 dna and is observed as a condensed, cross-linked structure when examined in the electron microscope by the formamide spreading technique. this structure appears to result from multiple cross-links induced in the tightly coiled dna contained within the spherical phix174 capsid. in contrast, the dna extracted after uv irradiation of the filamentous bacterio ... | 1972 | 5036669 |
| the proteins of bacteriophage m13. | particles of the small filamentous coliphage m13 contain not only the major coat protein, which is the product of phage gene 8, but also a minor coat protein, the a protein, which is the product of gene 3. the a protein has a molecular weight of approximately 70,000 daltons, is present in one copy per virion, and is responsible for phage attachment to host cells. also associated with purified m13 particles is a minor quantity of very small proteinaceous material, but its origin as a phage-coded ... | 1969 | 5257006 |
| purification and properties of diploid particles of coliphage m13. | 1967 | 5337710 | |
| enzymatic mechanisms of dna replication. | dna polymerases purified from several sources are characterized by replication of the 3'-hydroxy-terminated strand of a helical template. failure to achieve simultaneous replication of the 5'-strand leads to aberrations in the synthesized dna, described as nondenaturability and branching. aberrations in synthesized dna were not observed when (a) the 5'-strand was destroyed by a specific nuclease during the course of replication or (b) a single-stranded (circular) phage (m13) dna served as templa ... | 1966 | 5338562 |
| replication of bacteriophage m13. ii. the role of replicative forms in single-strand synthesis. | 1969 | 5401238 | |
| replication of bacteriophage m13. 3. identification of the intracellular single-straned dna. | 1969 | 5401239 | |
| replication of the small coliphage m13: evidence for long-living m13 specific messenger rna. | 1970 | 5422625 | |
| conditional lethal mutants of the small filamentous coliphage m13. ii. two genes for coat proteins. | 1969 | 5807970 | |
| conditional lethal mutants of the small filamentous coliphage m13. i. isolation, complementation, cell killing, time of cistron action. | 1966 | 5921643 | |
| ribonucleoprotein organization of eukaryotic rna. xxxi. structure of the u1 small nuclear ribonucleoprotein. | a small nuclear ribonucleoprotein, u1 snrnp, has been implicated in mrna processing. in this investigation sites of protein binding on u1 rna were mapped by nuclease protection and rna sequencing. partially purified human u1 snrnp was sequentially digested with escherichia coli rnaase iii and s1 nuclease. the resistant ribonucleoprotein fragments were deproteinized, preparatively hybridized to the u1 rna--complementary dna strand of a human u1 gene cloned in bacteriophage m13, and displayed by e ... | 1984 | 6084724 |
| use of short dna oligonucleotides for determination of dna sequence modifications induced by benzo[a]pyrene diol epoxide. | various organic agents that alkylate dna are known to induce mutations in bacterial and animal cells. the precise nature and location of modified dna sequences in such mutants are often difficult to ascertain. in this report, a 10-base-pair oligomer (bamhi linker) is treated with (+/-)-trans-benzo[a]pyrene-7,8-dihydrodiol-9,10-epoxide and inserted into replicative form dna of phage m13 by ligation at a specific restriction site. escherichia coli are transfected with the recombinant dna containin ... | 1984 | 6091121 |
| high-sensitivity s1 mapping with single-stranded [32p]dna probes synthesized from bacteriophage m13mp templates. | a method is described by which high-specific-activity single-stranded (ss) [alpha-32p]dna of a defined size complementary to sequences cloned into bacteriophage m13 is synthesized. the ss dna template is annealed with a universal sequencing primer, the primer extended with dna polymerase i klenow fragment and the dna duplex cut at a unique site 5' to the multiple cloning sites in the m13 phage. the reaction products are denatured and the ss alpha-32p probe fragment complementary to the cloned se ... | 1984 | 6096224 |
| integration of a temperate phage infecting spiroplasma citri. | a physical map of the genome of a temperate type 3 spiroplasma-virus, ai, has been constructed. host dna has been digested with restriction enzymes, and recombinant dna clones of ai fragments in coliphage m13 vectors have been used as probes to detect viral dna sequences integrated into spiroplasmas. all strains of spiroplasma citri examined contained a deleted form of ai integrated as a cryptic prophage which was unable to confer resistance to ai superinfection. stable ai lysogens also containe ... | 1984 | 6096304 |
| the gapped duplex dna approach to oligonucleotide-directed mutation construction. | a simple and efficient method is described to introduce structurally pre-determined mutations into recombinant genomes of filamentous phage m13. the method rests on gapped duplex dna (gddna) molecules of the phage m13 genome as the key intermediate. in this gddna, the (+) and the (shorter) (-) strand carry different genetic markers in such a way, that a rigorous selection can be applied for phage carrying the markers of the (-) strand. for introduction of the mutation, a synthetic oligonucleotid ... | 1984 | 6096830 |
| minimal size plasmids containing an m13 origin for production of single-strand transducing particles. | we have studied the requirements for efficient production of single-strand transducing particles from minimal size plasmids containing the phage m13 origin of replication. the most favorable origin fragment for production of transducing particles was found to extend from nucleotides 5372 to 5943 of the phage sequence, which includes a segment of the phage gene iv coding region and eliminates part of the gene ii promoter. minimizing vector size for m13 origin plasmids appears to be beneficial sin ... | 1984 | 6099398 |
| carboxy terminus of polyoma middle-sized tumor antigen is required for attachment to membranes, associated protein kinase activities, and cell transformation. | we have constructed a transformation-defective polyoma virus mutant (py 1387-t) that directs the synthesis of a normal small tumor antigen, a functional large tumor antigen, and a truncated (51,000-dalton) middle-sized tumor (mt) antigen that lacks 37 amino acids at its cooh terminus. the shortened mt polypeptide is missing the hydrophobic "tail" thought to be responsible for the anchorage of this protein into the plasma membrane and is in fact in cytosol fractions. this truncated mt polypeptide ... | 1982 | 6179082 |
| use of a rapid dna sequencing system to demonstrate the induction of frameshift mutations by bleomycin. | the rapid dna sequencing system based on the single-stranded bacteriophage m13 and the chain-terminator method has been used to look directly for mutational alterations. a small dna fragment that primes dna synthesis through the n-terminal 200 base pairs of the beta-galactosidase gene was prepared, and used to detect changes in base sequence among phages that give white plaques after treatment of the host cells with bleomycin. bleomycin treatment of e. coli in which m13 mp2 was growing gave an i ... | 1982 | 6183148 |
| decay of mrna in escherichia coli: investigation of the fate of specific segments of transcripts. | an assay was developed to investigate the fate of specific segments of beta-lactamase (bla) and ompa gene transcripts in escherichia coli. dna probes cloned in bacteriophage m13 were treated with an endonuclease capable of cleaving single-stranded dna, the fragments produced were annealed with total cellular rna, and the resulting rna . dna hybrids were subjected to s1 nuclease treatment and gel fractionation. by using this assay, direct evidence was obtained for 3'-to-5' directionality in the d ... | 1983 | 6187001 |
| rapid purification of bacterial plasmids and coliphage m13 rf without cscl centrifugation. | 1982 | 6187239 | |
| the nucleotide sequence of the akv murine leukemia virus genome. | the nucleotide sequence of an infectious molecular clone of the akv murine leukemia virus has been determined by the dideoxy chain termination method after subcloning in bacteriophage m13 vectors. the sequence predicts an rna genome of 8371 nucleotides containing three large open reading frames corresponding to the gag, pol, and env genes. signal sequences for transcription, splicing, and translation have been identified. the positions of 95 major rnase t1 resistant oligonucleotides of the akv r ... | 1984 | 6200992 |
| replication of bacteriophage m13. xv. location of the specific nick in m13 replicative form ii accumulated in escherichia coli polaex1. | m13 replicative form ii (rfii) dna was prepared from escherichia coli rs5052 (polaex1) cells in the late stage of infection, and the dna sequence at the discontinuity was examined. the data presented here suggest that the single discontinuity in the late stage of infection rfii maps at the same position as the gene ii protein nicking site on fd rfi which was determined in vitro (meyer et al., nature (london) 278:365-367, 1979) and has a 5' terminal nucleotide sequence identical to that at the ni ... | 1980 | 6246252 |
| template function of restriction enzyme fragments of phage m13 replicative form dna. | 1980 | 6246375 | |
| nucleotide sequence of the filamentous bacteriophage m13 dna genome: comparison with phage fd. | the 6407 nucleotide-long sequence of bacteriophage m13 dna has been determined using both the chemical degradation and chain-termination methods of dna sequencing. this sequence has been compared with that of the closely related bacteriophage fd (beck et al., 1978). m13 dna appears to be only a single nucleotide shorter than fd dna. there is an average of 3.0% of nucleotide-sequence differences between the two genomes, but the distribution of these changes is not random; the sequence of some gen ... | 1980 | 6254849 |
| construction and characterization of new coliphage m13 cloning vectors. | new single-stranded dna cloning vectors have been constructed by the insertion of additional dna fragments into a haeii restriction site in the bacteriophage m13 duplex replicative form (rf). these inserts into the m13 genome bring a single restriction sites useful for cloning, including psti, xorii, ecori, ssti, xhoi, kpni, and pvuii. drug-resistance genes cloned into m13 include the beta-lactamase (bla) gene and the chloramphenicol acetyl transferase (cat) gene. these vectors provide a conveni ... | 1980 | 6260570 |
| the dna sequences of cloned complex satellite dnas from hawaiian drosophila and their bearing on satellite dna sequence conservation. | a class of restriction endonuclease fragments near 185 bp in length and comprising approximately 20% of the genomes of 3 species of hawaiian drosophila has been cloned using bacteriophage m13. the nucleotide sequences of 14 clones have been determined and the variation between clones has been found to be due to deletions and base changes. analyses of uncloned material show that the cloning system itself does not introduce the variation. the variation of the basic repeat within and between specie ... | 1981 | 6262029 |
| [substrate specificity of ca2+,mg2+-dependent dnaase from sea urchin (strongylocentrotus intermedius) embryos]. | ca2+,mg2+-dependent dnase from sea urchin embryos is specific to the secondary structure of substrates irrespective of the nature of activating cations. the enzyme does not split synthetic single-stranded oligo and polynucleotides, such as d(ptptptpcpc), d(pgpgptptpt). d(papaptptpc), d(pgpapaptptpc), d(pa)5-poly(dt), d(papaptptpc)-poly(dt), poly(da) and poly (dt) and hydrolyses the double-stranded substrates poly d(at), poly (da) . poly (dt) and highly polymerized dna. native double-stranded dna ... | 1981 | 6271260 |
| the atp operon: nucleotide sequence of the region encoding the alpha-subunit of escherichia coli atp-synthase. | part of the atp (or unc) operon encoding the alpha, beta, gamma, delta, and epsilon subunits of escherichia coli atp-synthase has been cloned into the plasmid pacyc 184. the dna coding for the largest of these proteins, the alphas subunit, has been sequenced by cloning into the bacteriophage m13 and sequencing with dideoxy nucleotide chain terminators. it comprises 1539 nucleotides corresponding to a protein of 513 amino acids. | 1981 | 6272228 |
| viable deletions of the m13 complementary strand origin. | the single-stranded dna of bacteriophage m13 is converted to a duplex replicative form by a mechanism involving rna-primed initiation at a single unique site on the viral dna. the dna sequence that specifies the rna primer is contained largely within one of two adjacent hairpin structures protected from dnase degradation by rna polymerase. we have used in vitro techniques to construct a series of m13 mutants having deletions in the region of the complementary strand origin. deletions of the dupl ... | 1981 | 6273888 |
| oligonucleotide-directed mutagenesis of gene ix of bacteriophage m13. | the synthetic oligodeoxyribonucleotide pcgaaagactacac has been applied as a site-specific mutagen to introduce a t leads to g transversion mutation at nucleotide position 1223 of the m13 dna sequence. the in vitro-induced conversion of a tat codon into a tag at this position resulted in gene ix mutants with an amber mutant character thereby confirming that this reading frame defines a gene of an essential phage protein. the gene ix amber mutants obtained grew well on sui (ser) and suiii (tyr) su ... | 1982 | 6278437 |
| sequencing long dna fragments cloned in bacteriophage m13 by using internal primers. the sequence analysis of a yeast dna fragment containing a replication origin. | in the ;shotgun' procedure for sequencing dna, dna fragments are cloned into a phage m13 vector and sequenced by using a flanking primer. in a variation of this procedure a longer dna sequence is cloned into m13, the two single-stranded recombinants identified and sequenced by using a set of internal primers prepared by exonuclease iii digestion of restriction fragments. | 1981 | 6280678 |
| construction of gapped circular dna from phage m13 by in vitro hybridization. | 1982 | 6282331 | |
| cloning and transcriptional control of a eucaryotic permease gene. | the uracil permease gene of the yeast saccharomyces cerevisiae was cloned on a hybrid plasmid which replicates autonomously in both yeast and escherichia coli. cloning was carried out by complementation in yeast. the smallest dna fragment found to complement the uracil permease deficiency in recipient yeast cells measured approximately 2.3 kilobases. in strains transformed by the plasmid with the uracil permease gene inserted, initial rates of uracil uptake increased up to 25 times more than the ... | 1982 | 6290876 |
| nucleotide sequence of bacteriophage f1 dna. | the nucleotide sequence of the dna of the filamentous coliphage f1 has been determined. in agreement with earlier conclusions, the genome was found to comprise 6,407 nucleotides, 1 less than that of the related phage fd. phage f1 dna differs from that of phage m13 by 52 nucleotide changes, which lead to 5 amino acid substitutions in the corresponding proteins of the two phages, and from phage fd dna by 186 nucleotide changes (including the single-nucleotide deletion), which lead to 12 amino acid ... | 1982 | 6292494 |
| sandwich hybridization as a convenient method for the detection of nucleic acids in crude samples. | a method based on three-dna-component, sandwich hybridization has been designed for the detection and quantitation of nucleic acids in crude samples using adenovirus dna as a model. two non-overlapping restriction fragments of adenovirus type 2 (ad2) dna were cloned into two vectors, the pbr322 plasmid and m13 phage. the recombinant plasmid dna was immobilized onto nitrocellulose filters and the single-stranded recombinant phage dna was labeled with 125i and used as a probe. when these two reage ... | 1983 | 6301952 |
| construction of a cloned library of adenovirus dna fragments in bacteriophage m13. | the construction of recombinant m13 phages containing adenovirus dna inserts was undertaken to provide strand-specific hybridization probes for analyses of adenovirus type 2 rna transcripts. a library of molecular probes was constructed by cloning restriction endonuclease fragments of adenovirus types 2 and 5 dna in the duplex replicative form dna of the single-stranded bacteriophage vectors, m13mp7, m13mp8, and m13mp9 (messing, j., and vieira, j. (1982) gene 19,269-276). adenovirus dna segments ... | 1983 | 6309766 |
| microinjected simian virus 40 crna is spliced, as evidenced by electron microscopy. | simian virus 40 crna was transcribed in vitro from the early viral dna strand. the rna was injected through glass capillaries into the nuclei of monkey cells. after a 2-h incubation, the rnas were extracted and hybridized to single-stranded simian virus 40 dna sequences contained in a bacteriophage m13 vector. electron microscopy revealed processed crnas with splice loops in the region of the intron of large t antigen. | 1983 | 6310149 |
| kilo-sequencing: creation of an ordered nest of asymmetric deletions across a large target sequence carried on phage m13. | 1983 | 6310345 | |
| expression of the human interferon-beta gene cloned in phage m13 mp7. | the single-stranded dna phage, m13 mp7 was used in the construction of an expression vector containing the coding sequence for mature interferon-beta (ifn-beta). two clones expressed a fused polypeptide showing the biological and physicochemical properties of ifn-beta, despite the fact that the n-terminal amino acid sequence had been changed; 10(6) i.u./l of culture were produced with a molecular weight of 20 000. | 1983 | 6311267 |
| characterization of the dna binding protein encoded by the n-specific filamentous escherichia coli phage ike. binding properties of the protein and nucleotide sequence of the gene. | a dna binding protein encoded by the filamentous single-stranded dna phage ike has been isolated from ike-infected escherichia coli cells. fluorescence and in vitro binding studies have shown that the protein binds co-operatively and with a high specificity to single-stranded but not to double-stranded dna. from titration of the protein to poly(da) it has been calculated that approximately four bases of the dna are covered by one monomer of protein. these binding characteristics closely resemble ... | 1983 | 6312049 |
| template requirements for the initiation of adenovirus dna replication. | the first step in the replication of the adenovirus genome is the covalent attachment of the 5'-terminal nucleotide, dcmp, to the virus-encoded terminal protein precursor (ptp). this reaction can be observed in vitro and has been previously shown to be dependent upon either viral dna or linearized plasmid dna containing viral terminal sequences. plasmids containing deletions or point mutations within the viral terminal sequence were constructed by site-directed mutagenesis. in the case of linear ... | 1984 | 6320160 |
| replication functions of pc194 are necessary for efficient plasmid transduction by m13 phage. | escherichia coli plasmids pbr313 and pbr322 were transduced by phage m13 with low efficiency (10(-8) transductants/phage). hybrid plasmids phv12 or phv33, composed of staphylococcus aureus plasmid pc194 and pbr313 or pbr322, respectively, were transduced much more efficiently (10(-4) transductants/phage). inactivation of either of the two zones necessary for pc194 replication, one coding for a protein, the other not, reduced the transforming efficiency of hybrids to the level of pbr322. activity ... | 1984 | 6323171 |
| genes involved in transitory recombination between phage m13 and plasmid phv33. | plasmid phv33 and phage m13 combine in escherichia coli cells to form a chimera, which decombines to regenerate two parental genomes. combination can occur via two genetic pathways, one defined by the recbc genes, the other by reca, recf and possibly recl genes. decombination can also occur via two pathways, one defined again by the recbc genes, the other by a gene not identified, but active only in the absence of the recl gene product. | 1984 | 6323172 |