Publications

infectivity, effects on helper viruses and whitefly transmission of the deltasatellites associated with sweepoviruses (genus begomovirus, family geminiviridae).	begomoviruses (family geminiviridae) are whitefly-transmitted viruses with single-stranded dna genomes that are frequently associated with dna satellites. these satellites include non-coding satellites, for which the name deltasatellites has been proposed. although the first deltasatellite was identified in the late 1990s, little is known about the effects they have on infections of their helper begomoviruses. recently a group of deltasatellites were identified associated with sweepoviruses, a g ...	2016	27453359
the whitefly bemisia tabaci knottin-1 gene is implicated in regulating the quantity of tomato yellow leaf curl virus ingested and transmitted by the insect.	the whitefly bemisia tabaci is a major pest to agricultural crops. it transmits begomoviruses, such as tomato yellow leaf curl virus (tylcv), in a circular, persistent fashion. transcriptome analyses revealed that b. tabaci knottin genes were responsive to various stresses. upon ingestion of tomato begomoviruses, two of the four knottin genes were upregulated, knot-1 (with the highest expression) and knot-3. in this study, we examined the involvement of b. tabaci knottin genes in relation to tyl ...	2016	27455309
tomato yellow leaf curl virus: no evidence for replication in the insect vector bemisia tabaci.	begomovirus ssdna plant virus (family geminiviridae) replication within the bemisia tabaci vector is controversial. transovarial transmission, alteration to whitefly biology, or detection of viral transcripts in the vector are proposed as indirect evidence of replication of tomato yellow leaf curl virus (tylcv). recently, contrasting direct evidence has been reported regarding the capacity of tylcv to replicate within individuals of b. tabaci based on quantitave pcr approaches. time-course exper ...	2016	27476582
next-generation sequencing and genome editing in plant virology.	next-generation sequencing (ngs) has been applied to plant virology since 2009. ngs provides highly efficient, rapid, low cost dna, or rna high-throughput sequencing of the genomes of plant viruses and viroids and of the specific small rnas generated during the infection process. these small rnas, which cover frequently the whole genome of the infectious agent, are 21-24 nt long and are known as vsrnas for viruses and vd-srnas for viroids. ngs has been used in a number of studies in plant virolo ...	2016	27617007
ongoing geographical spread of tomato yellow leaf curl virus.	tomato yellow leaf curl virus (tylcv) seriously impacts tomato production throughout tropical and sub-tropical regions of the world. it has a broad geographical distribution and continues to spread to new regions in the indian and pacific oceans including australia, new caledonia and mauritius. we undertook a temporally-scaled, phylogeographic analysis of all publicly available, full genome sequences of tylcv, together with 70 new genome sequences from australia, iran and mauritius. this reveale ...	2016	27619929
effects of isaria fumosorosea on tylcv (tomato yellow leaf curl virus) accumulation and transmitting capacity of bemisia tabaci.	tomato yellow leaf curl virus (tylcv) is transmitted by the bemisia tabaci pest middle east-asia minor 1 (meam1) in china. isaria fumosorosea is a fungal pathogen of b. tabaci. however, the effects of fungal infection on tylcv expression and transmission by meam1 are unclear. in this study, potted tomatoes containing second instar nymphs of meam1 were treated with i. fumosorosea ifb01 strain and the relationship between fungal infection in meam1 and its tylcv transmission capacity was investigat ...	2016	27716852
members of wrky group iii transcription factors are important in tylcv defense signaling pathway in tomato (solanum lycopersicum).	transmitted by the whitefly bemisia tabaci, tomato yellow leaf curly virus (tylcv) has posed serious threats to plant growth and development. plant innate immune systems against various threats involve wrky group iii transcription factors (tfs). this group participates as a major component of biological processes in plants.	2016	27717312
comparative analyses of tomato yellow leaf curl virus c4 protein-interacting host proteins in healthy and infected tomato tissues.	tomato yellow leaf curl virus (tylcv), a member of the genus begomovirus, is one of the most important viruses of cultivated tomatoes worldwide, mainly causing yellowing and curling of leaves with stunting in plants. tylcv causes severe problems in sub-tropical and tropical countries, as well as in korea. however, the mechanism of tylcv infection remains unclear, although the function of each viral component has been identified. tylcv c4 codes for a small protein involved in various cellular fun ...	2016	27721687
the contrasting effects of elevated co2 on tylcv infection of tomato genotypes with and without the resistance gene, mi-1.2.	elevated atmospheric co2 typically enhances photosynthesis of c3 plants and alters primary and secondary metabolites in plant tissue. by modifying the defensive signaling pathways in host plants, elevated co2 could potentially affect the interactions between plants, viruses, and insects that vector viruses. r gene-mediated resistance in plants represents an efficient and highly specific defense against pathogens and herbivorous insects. the current study determined the effect of elevated co2 on ...	2016	27881989
a peptidoglycan recognition protein acts in whitefly (bemisia tabaci) immunity and involves in begomovirus acquisition.	peptidoglycan recognition proteins (pgrps) are multifunctional pattern recognition proteins. here, we report that a pgrp gene, btpgrp, encodes a pgrp from the whitefly bemisia tabaci (meam1) that binds and kills bacteria in vitro. we analyzed btpgrp transcriptional profiling, and the distribution of the cognate protein within the midgut. fungal infection and wasp parasitization induced expression of btpgrp. silencing btpgrp with artificial media amended with dsrna led to reduced expression of a ...	2016	27892529
implication of the whitefly bemisia tabaci cyclophilin b protein in the transmission of tomato yellow leaf curl virus.	tomato yellow leaf curl virus (tylcv) is a single-stranded (ssdna) begomoviruses that causes severe damage to tomato and several other crops worldwide. tylcv is exclusively transmitted by the sweetpotato whitefly, bemisia tabaci in a persistent circulative and propagative manner. previous studies have shown that the transmission, retention, and circulation of tylcv in its vector involves interaction with insect and endosymbiont proteins, which aid in the transmission of the virus, or have a prot ...	2016	27895657
ap2/erf transcription factors involved in response to tomato yellow leaf curly virus in tomato.	tomato yellow leaf curly virus (tylcv), transmitted by the whitefly (), causes leaf curling and yellowing, plant dwarfism, and growth inhibition in tomato ( l.). the apetala2 (ap2) and ethylene response factor (erf) transcription factor (tf) family, the largest plant-specific tf family, was identified to function in plant development and pathogen defense. our study aimed to analyze the mechanism underlying the function of erf (slerf) tfs in response to tylcv infection and improve useful informat ...	2016	27898839
the non-canonical tomato yellow leaf curl virus recombinant that displaced its parental viruses in southern morocco exhibits a high selective advantage in experimental conditions.	recombination events are frequently inferred from the increasing number of sequenced viral genomes, but their impact on natural viral populations has rarely been evidenced. tylcv-is76 is a recombinant (begomovirus,geminiviridae) between the israel strain of tomato yellow leaf curl virus (tylcv-il) and the spanish strain of tomato yellow leaf curl sardinia virus (tylcsv-es) that was generated most probably in the late 1990s in southern morocco (souss). its emergence in the 2000s coincided with th ...	2016	27902403
elevated o₃ and tylcv infection reduce the suitability of tomato as a host for the whitefly bemisia tabaci.	the effects of elevated atmospheric ozone (o₃) levels on herbivorous insects have been well studied, but little is known about the combined effects of elevated o₃ and virus infection on herbivorous insect performance. using open-top chambers in the field, we determined the effects of elevated o₃ and tomato yellow leaf curl virus (tylcv) infection on wild-type (wt) tomato and 35s tomato (jasmonic acid (ja) defense-enhanced genotype) in association with whitefly, bemisia tabaci gennadius biotype b ...	2016	27916792
a jasmonate-inducible defense trait transferred from wild into cultivated tomato establishes increased whitefly resistance and reduced viral disease incidence.	whiteflies damage tomatoes mostly via the viruses they transmit. cultivated tomatoes lack many of the resistances of their wild relatives. in order to increase protection to its major pest, the whitefly bemisia tabaci and its transmitted tomato yellow leaf curl virus (tylcv), we introgressed a trichome-based resistance trait from the wild tomato solanum pimpinellifolium into cultivated tomato, solanum lycopersicum. the tomato backcross line bc5s2 contains acylsucrose-producing type-iv trichomes, ...	2016	27920785
clathrin-mediated endocytosis is involved in tomato yellow leaf curl virus transport across the midgut barrier of its whitefly vector.	tomato yellow leaf curl virus (tylcv) is a begomovirus transmitted by the whitefly bemisia tabaci. the circulative translocation of the virus in the insect is known in its broad line. however, transit of tylcv from the digestive tract into the haemolymph is poorly understood. we studied the involvement of clathrin in this process by disrupting the clathrin-mediated endocytosis and the endosome network using inhibitor feeding, antibody blocking and dsrna silencing. we monitored the quantities of ...	2017	28056414
virus surveys of capsicum spp. in the republic of benin reveal the prevalence of pepper vein yellows virus and the identification of a previously uncharacterised polerovirus species.	surveys were conducted in 2014 and 2015 in southern and northern benin, respectively, to identify the viruses infecting peppers (capsicum spp.). the samples were screened by elisa for cucumber mosaic virus (cmv), pepper veinal mottle virus (pvmv), potato virus y (pvy) and tomato yellow leaf curl virus (tylcv). a generic reverse transcription pcr (rt-pcr) was used to test for the presence of poleroviruses. elisa tests confirmed the prevalence of all viruses, while the rt-pcr detected pepper vein ...	2017	28220324
slmapk3 enhances tolerance to tomato yellow leaf curl virus (tylcv) by regulating salicylic acid and jasmonic acid signaling in tomato (solanum lycopersicum).	several recent studies have reported on the role of mitogen-activated protein kinase (mapk3) in plant immune responses. however, little is known about how mapk3 functions in tomato (solanum lycopersicum l.) infected with tomato yellow leaf curl virus (tylcv). there is also uncertainty about the connection between plant mapk3 and the salicylic acid (sa) and jasmonic acid (ja) defense-signaling pathways. the results of this study indicated that slmapk3 participates in the antiviral response agains ...	2017	28222174
autophagy functions as an antiviral mechanism against geminiviruses in plants.	autophagy is an evolutionarily conserved process that recycles damaged or unwanted cellular components, and has been linked to plant immunity. however, how autophagy contributes to plant immunity is unknown. here we reported that the plant autophagic machinery targets the virulence factor βc1 of cotton leaf curl multan virus (clcumuv) for degradation through its interaction with the key autophagy protein atg8. a v32a mutation in βc1 abolished its interaction with nbatg8f, and virus carrying βc1( ...	2017	28244873
the six tomato yellow leaf curl virus genes expressed individually in tomato induce different levels of plant stress response attenuation.	tomato yellow leaf curl virus (tylcv) is a begomovirus infecting tomato plants worldwide. tylcv needs a healthy host environment to ensure a successful infection cycle for long periods. hence, tylcv restrains its destructive effect and induces neither a hypersensitive response nor cell death in infected tomatoes. on the contrary, tylcv counteracts cell death induced by other factors, such as inactivation of hsp90 functionality. suppression of plant death is associated with the inhibition of the ...	2017	28324352
impact of cis-acting elements' frequency in transcription activity in dicot and monocot plants.	the production of new cultivars via recombinant dna technology is important in applied agriculture. promoters play fundamental roles in successful transformation and gene expression. fragments of the upstream regulatory region of the movement protein gene of the tomato yellow leaf curl virus (tylcv; two fragments) and watermelon chlorotic stunt virus (wmcsv, two fragments) and one fragment of the coat protein putative promoter of tylcv (cpty-pro) were isolated to assess their abilities to drive ...	2015	28324408
diversity, distribution, and evolution of tomato viruses in china uncovered by small rna sequencing.	tomato is a major vegetable crop that has tremendous popularity. however, viral disease is still a major factor limiting tomato production. here we report the tomato virome identified through sequencing small rnas of 170 field-grown samples collected in china. a total of 22 viruses were identified including both well-documented and newly detected viruses. the tomato viral community is dominated by a few species, and they exhibit polymorphisms and recombination in the genomes with coldspots and h ...	2017	28331089
insecticidal effects on the spatial progression of tomato yellow leaf curl virus and movement of its whitefly vector in tomato.	commercial management of whitefly-transmitted tomato yellow leaf curl virus (tylcv) typically relies on insecticide control of whitefly vectors as a first line of defense. we quantified this effect in crop tunnel studies, with validation in a tomato field setting. tomato yellow leaf curl virus-infected and bemisia tabaci (gennadius)-infested source plants were planted at the beginning of tunneled rows to serve as inoculum source, so that movement of whiteflies and tylcv symptoms could be tracked ...	2017	28334339
flupyradifurone effectively manages whitefly bemisia tabaci med (hemiptera: aleyrodidae) and tomato yellow leaf curl virus (tylcv) in tomato.	the cotton whitefly bemisia tabaci (gennadius) is among the most important pests of numerous crops and a vector of >100 plant viruses, causing significant crop losses worldwide. managing the pest as well as inhibiting the transmission of major viruses such as tomato yellow leaf curl virus (tylcv) is of utmost importance for sustainable yields. the efficacy on both whiteflies and virus transmission of the novel systemic butenolide insecticide flupyradifurone, was investigated in this study.	2017	28345196
odor, not performance, dictates bemisia tabaci's selection between healthy and virus infected plants.	although, insect herbivores are generally thought to select hosts that favor the fitness of their progeny, this "mother-knows-best" hypothesis may be challenged by the presence of a plant virus. our previous study showed that the whitefly, bemisia tabaci, the obligate vector for transmitting tomato yellow leaf curl virus (tylcv), preferred to settle and oviposit on tylcv-infected rather than healthy host plant, datura stramonium. the performances of b. tabaci larvae and adults were indeed improv ...	2017	28360861
the involvement of heat shock proteins in the establishment of tomato yellow leaf curl virus infection.	tomato yellow leaf curl virus (tylcv), a begomovirus, induces protein aggregation in infected tomatoes and in its whitefly vector bemisia tabaci. the interactions between tylcv and hsp70 and hsp90 in plants and vectors are necessity for virus infection to proceed. in infected host cells, hsp70 and hsp90 are redistributed from a soluble to an aggregated state. these aggregates contain, together with viral dna/proteins and virions, hsps and components of the protein quality control system such as ...	2017	28360921
no evidence of seed transmissibility of tomato yellow leaf curl virus in nicotiana benthamiana.	recent work reported that tomato yellow leaf curl virus (tylcv) is seed-transmissible in tomato, contrary to previous belief. in this work, we explore whether tylcv is also a seed-borne virus in another member of the solanaceae family, the experimental host nicotiana benthamiana.	2017	28471116
vector development and vitellogenin determine the transovarial transmission of begomoviruses.	the majority of plant viruses are transmitted by insect vectors between hosts, and transovarial transmission of viruses from vector parents to offspring has great significance to their epidemiology. begomoviruses are transmitted by the whitefly bemisia tabaci in a circulative manner and are maintained through a plant-insect-plant cycle. other routes of begomovirus transmission are not clearly known. here, we report that transovarial transmission from female whiteflies to offspring often happens ...	2017	28607073
towards area wide management of insect vectored viruses of tomatoes in the bowen district.	the bowen region of northern queensland is an important winter production area for tomatoes in australia. there are three economically important viruses in the region that affect tomato, tomato yellow leaf curl virus (tylcv), tomato spotted wilt virus (tswv) and potato leafroll virus (plrv), which are vectored by whiteflies, thrips and aphids, respectively. an area wide management approach is required to lower the primary inoculum throughout the district. to this end, we undertook investigations ...	2017	28625668
expression of tomato yellow leaf curl virus coat protein using baculovirus expression system and evaluation of its utility as a viral antigen.	dna encoding the coat protein (cp) of an egyptian isolate of tomato yellow leaf curl virus (tylcv) was inserted into the genome of autographa californica nucleopolyhedrovirus (acnpv) under the control of polyhedrin promoter. the generated recombinant baculovirus construct harboring the coat protein gene was characterized using pcr analysis. the recombinant coat protein expressed in infected insect cells was used as a coating antigen in an indirect enzyme-linked immunosorbent assay (elisa) and do ...	2017	28794924
seed transmission of tomato yellow leaf curl virus in white soybean (glycine max).	tomato yellow leaf curl virus (tylcv) infection of the common bean (phaseolus vulgaris) has been reported, but soybean (glycine max) has not previously been identified as a tylcv host. five cultivars of white soybean were agro-inoculated using an infectious tylcv clone. at 30 days post-inoculation, they showed infection rates of 25% to 100%. typical tylcv symptoms were not observed in any inoculated plants. to examine whether tylcv was transmitted in soybean seeds, dna was isolated from bundles ...	2017	28811759
detection and molecular characterization of tomato yellow leaf curl virus naturally infecting lycopersicon esculentum in egypt.	tomato yellow leaf curl virus (tylcv) infections of tomato crops in egypt were widely spread in 2014. infected symptomatic tomato plants from different governorates were sampled. tylcv strains israel and mild (tylcv-il, tylcv-mild) were identified by multiplex and real-time pcr. in addition, nucleotide sequence analysis of the v1 and v2 protein genes, revealed ten tylcv egyptian isolates (tylcv from ty1 to 10). phylogenetic analysis showed their high degree of relatedness with tylcv-il jordan is ...	2017	28854789