Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| separation of marek's disease virus dna by field inversion gel electrophoresis. | 1990 | 1962833 | |
| a unique region of marek's disease virus dna in a virus-nonproductive lymphoblastoid cell line, mdcc-rp1. | 1990 | 1962835 | |
| [molecular biologic study of marek's disease virus and ebv]. | 1990 | 1962969 | |
| cell culture amplification of a defective marek's disease virus. | a highly amplified 4-kb ecori fragment was present in dna isolated from high cell culture passaged stocks (greater than 93 passages) of 281mi/1, a serotype 2 marek's disease virus (mdv). the isolated 4-kb fragment is amplified in the presence of mdv, replicating as a high molecular weight, head-to-tail concatemer. when the 4-kb fragment was cloned into puc18 and cotransfected with mdv dna into chicken embryo fibroblast cells, the plasmid clone also replicated as a high molecular weight concateme ... | 1990 | 1980176 |
| [the effect of cyadox and virginiamycin on marek's disease in chickens]. | chickens infected with highly virulent marek's disease virus were administered in the course of 56-day fattening cyadox at a dose of 20 mg per kg feed mixture (mc group). and virginiamycin at a dose of 10 mg per kg feed mixture (mv group). the weight gains on the 56th day and dressing percentage were highest, in comparison with the control group (711.11 g and 60.62%), in the virginiamycin-treated group. an investigation into the basic chemical composition of meat revealed lower contents of dry m ... | 1990 | 2112800 |
| detection of marek's disease virus antigen in chickens by a novel immunoassay. | an immunoassay was developed to detect marek's disease virus (mdv) antigen on the tips of feathers obtained from mdv-infected chickens. mdv in follicular debris on the feather tip was demonstrated by use of a specific monoclonal antibody. the principle of an indirect elisa was employed and the feather tip was used as the solid phase. presence of mdv was reflected by a dark brown precipitate on the feather tip which could be observed by naked eye. this test system proved to be more sensitive than ... | 1990 | 2156882 |
| purification of genomic sized herpesvirus dna using pulse-field electrophoresis. | a new method for the separation of genomic sized herpesvirus dna was developed. this method utilizes the improved separation of extremely high molecular weight dna molecules by pulse-field electrophoresis. this method has allowed us to separate and purify herpesvirus dna (greater than 100 kbp) free of cellular dna, directly from infected cells. in the case of cell associated herpesviruses (such as marek's disease herpesvirus), this method is more efficient than present isolation procedures. the ... | 1990 | 2157730 |
| evaluation of pathogenicity and protective efficacy of serotype 2 marek's disease virus from birds belonging to genus gallus in japan. | the new cloned serotype 2 marek's disease viruses (mdv) of ml-6, ml-9, and ml-22 strains were inoculated in specific-pathogen-free (spf) chicks to evaluate the pathogenicity and protective efficacy. chicks inoculated or contact-infected with ml strains showed no gross and histological lesions in lymphoid organs, sciatic plexuses and other visceral organs during 10 weeks of observation periods, indicating that the viruses were non-pathogenic. moreover, the viruses were found to be spread horizont ... | 1990 | 2161478 |
| characterization of the gene encoding herpesvirus of turkeys gp57-65: comparison to marek's disease virus gp57-65 and herpes simplex virus glycoprotein c. | a gene encoding herpesvirus of turkeys (hvt) strain fc 126 gp57-65 has been mapped to the viral genome and sequenced. the hvt (fc 126) gp57-65 gene maps to bamhi fragments k1 and m, colinear with the gene from marek's disease virus (mdv) strain ga. hvt gp57-65 gene sequences were compared to the mdv strain ga gp57-65 gene that we sequenced previously. overall, the two sequences are 66% identical, with greater similarity in the 3' proximal two thirds of the genes. hvt gp57-65 gene sequences have ... | 1990 | 2161583 |
| isolation of serotype 2 marek's disease virus from birds belonging to genus gallus in japan. | serotype 2 of marek's disease virus (mdv) was isolated from apparently healthy birds belonging to genus gallus that had no history of vaccination with mdv or herpesvirus of turkeys (hvt). buffy-coat cells from these birds were inoculated onto chicken embryo fibroblast (cef) cultures for primary isolation. thirteen isolates from one golden pheasant and three white silky fowls, three black silky fowls, three japanese long crowers, and three japanese bantams produced herpes-like cytopathic effects ... | 1990 | 2164389 |
| transfection of chicken embryo fibroblasts with marek's disease virus dna. | total dna from marek's disease virus (mdv)-infected chicken embryo fibroblasts was transfected into freshly plated secondary chicken embryo fibroblasts using calcium phosphate-mediated transfection. transfection frequencies were dose-dependent and non-linear. the maximum transfection frequencies of nine mdv dna preparations using 8-25 micrograms total dna ranged from 45 to 898 plaques per calcium phosphate/dna precipitate. approximately 100-200 plaques per 60-mm tissue-culture dish using 1-5 mic ... | 1990 | 2164390 |
| identification of the cross-reactive antigens between marek's disease virus and pseudorabies virus by monoclonal antibodies. | among the 33 monoclonal antibodies (mabs) against pseudorabies virus (prv) examined, three mabs (24-17, 74-26, and 8) were found to react with cells infected with marek's disease virus (mdv)-related viruses by immunofluorescence test. two of the mabs (24-17 and 74-26) reacted with the nuclei of cells infected with mdv serotype 1 (mdv1), mdv serotype 2 (mdv2), and herpesvirus of turkeys (hvt), whereas mab 8 reacted with the cytoplasm of mdv2- and hvt-infected cells. however, none of the mabs agai ... | 1990 | 2164393 |
| synthesis and processing of a gp28/32 membrane glycoprotein induced by marek's disease virus serotype 2. | the post-translational events leading from the precursor to the processed forms of a glycoprotein with an mr of 28k to 32k (gp27/32) of marek's disease virus (mdv) serotype 2 were examined with pulse-chase experiments and treatment with tunicamycin and monensin. cell-free translation of infected cell mrna followed by immunoprecipitation analysis suggested that a polypeptide with a size of 22k is the initial precursor. experiments with endo-beta-n-acetylglucosaminidase h and endo-beta-n-acetylglu ... | 1990 | 2167934 |
| isolation of very virulent marek's disease viruses from vaccinated chickens in australia. | very virulent marek's disease viruses (vvmdv), defined as isolates against which the herpesvirus of turkey (hvt) vaccine provide poor protection, have been isolated from poultry flocks in both the united states and europe. twenty-one samples from vaccinated australian flocks, experiencing problems with excessive marek's disease (md), were tested for the presence of transmissible md viruses (mdv). of the 16 samples which contained a transmissible agent, 14 were pathogenic in chickens, based on th ... | 1990 | 2171476 |
| production of marek's disease vaccine. | marek's disease (md) is one of the viral infections that attack poultry and are widespread throughout the world. vaccination is widely used for the protection of chickens against outbreaks of md, and commercial vaccines have been used since 1970. there are vaccines derived from three avian herpesvirus serotypes which are propagated in cef or def cell cultures. most of them are used successfully for vaccination against mdv and are effective and safe. in problem areas where the monovalent vaccine ... | 1990 | 2171556 |
| transactivation of the rous sarcoma virus long terminal repeat promoter by marek's disease virus. | transient expression of chloramphenicol acetyltransferase (cat) was used to study marek's diseases virus (mdv)-mediated transactivation of the rous sarcoma virus long terminal repeat (rsv-ltr) promoter. cotransfection experiments in primary avian cells were conducted using mdv high-molecular-weight dna and plasmid prsvcat. increased cat activity, relative to controls, was consistently observed in the presence of mdv. enhanced cat activity, expressed via the rsv-ltr promoter, was strictly depende ... | 1990 | 2173259 |
| replicating marek's disease virus (mdv) serotype 2 dna with inserted mdv serotype 1 dna sequences in a marek's disease lymphoblastoid cell line msb1-41c. | we characterized the properties of herpes-type viruses which grew well in a marek's disease lymphoblastoid cell line, msb1-41c, inducing cytopathic effect characterized by the formation of syncytial giant cells. examination of the infectious virus by field inversion gel electrophoresis revealed the presence of dna of about 180 kbp in both the culture fluid and cell fractions of the infected msb-41c cells. the dna was found to consist of marek's disease virus (mdv) serotype 2 (mdv2) and mdv serot ... | 1990 | 2173523 |
| [viruses and atherosclerosis]. | our understanding of the pathogenesis and aetiology of atherosclerosis remains incomplete. an infection by herpesviruses is among the plausible hypotheses. in chicken, inoculation with marek's disease virus provokes arterial lesions closely resembling atherosclerotic plaques. experiments done on cultured arterial cells have shown that herpesviruses might enhance atherogenesis. herpesviruses molecules (proteins, messenger ribonucleic acid, deoxyribonucleic acid) are commonly found in the human ar ... | 1990 | 2175447 |
| establishment and characterization of a chicken mononuclear cell line. | a new chicken mononuclear cell line (mq-ncsu) has been established. the starting material used to initiate this cell line was a transformed spleen from a female dekalb xl chicken which had been experimentally challenged with the jm/102w strain of the marek's disease virus. after homogenization, a single cell suspension of splenic cells was cultured using l.m. hahn medium supplemented with 10 microm 2-mercaptoethanol. under these culture conditions, a rapidly proliferating cell was observed and t ... | 1990 | 2176014 |
| identification of virus-specific antigens in cultured cells infected with marek's disease virus serotype 2 and cvi-988 strain. | for the identification of serotype-specific antigens of marek's disease virus (mdv) serotype 1 (mdv1) or serotype 2 (mdv2), a total of 24 hybridoma clones, secreting monoclonal antibodies (mabs) against cvi-988 (mdv1) or hprs-24 (mdv2) strain, were established and characterized by immunofluorescence assay, virus neutralization and immunoprecipitation analysis. based upon the molecular weights (mol. wt.) of the immunoprecipitated polypeptides, the mabs were subdivided into 7 groups. among them, t ... | 1990 | 2177808 |
| biological diversity among serotype 2 marek's disease viruses. | selected biological characteristics were determined for 14 low-passage serotype 2 marek's disease virus (mdv) isolates. four of these isolates were also tested after extensive serial passage in chicken embryo fibroblast cultures. observations were made on replication in vitro and in vivo, pathogenicity by in ovo inoculation, antigenicity, and protection against virulent mdv challenge. among the low-passage isolates, there were some differences in pathogenicity after in ovo inoculation but relati ... | 1990 | 2177978 |
| identification of protein complexes containing the c-rel proto-oncogene product in avian hematopoietic cells. | the c-rel proto-oncogene product has been identified as a 75 kda protein expressed in lymphoid cells transformed by rev-t and marek's disease virus. a 4.0 kb c-rel transcript is expressed in the bursa, spleen and thymus of chickens with highest levels of expression at 10 days post hatch. using antiserum specific for the v-rel oncogene product, p75c-rel has been precipitated from [35s]methionine-labeled extracts of bursal, splenic and thymic lymphocytes. additionally, proteins with the molecular ... | 1990 | 2202947 |
| the effect of serologically defined major histocompatibility complex haplotypes on marek's disease resistance in commercially bred white leghorn chickens. | in commercial pure white leghorn lines, a, b, and c, the effects on resistance against a virulent strain of marek's disease virus were assessed for b19 and b21 haplotypes of the chicken major histocompatibility complex. b haplotypes were identified by direct hemagglutination using alloantisera raised against erythrocyte antigens. in homozygous b21 female chicks from lines a and b, mortality upon challenge with virus was 16% and 9%, respectively; in b19 chicks, mortality was 42% and 60%, respecti ... | 1990 | 2282011 |
| similarities and dissimilarities in the structure and expression of viral genomes of various virus strains immunologically related to marek's disease virus. | various strains immunologically related to marek's disease virus (mdv) have been subdivided into three serotypes: serotype 1, pathogenic strains of mdv and attenuated or apathogenic variants derived from them; serotype 2, naturally occurring apathogenic strains of mdv; serotype 3, herpesvirus of turkey (hvt). the viral genome structures of these three serotypes were compared by a simple, practical method using total dna extracted from virus-infected cells instead of viral dna purified from virio ... | 1986 | 2424404 |
| monoclonal antibody to chicken fetal antigen on marek's disease lymphoblastoid cell line mdcc-msb1. | a monoclonal antibody (2h3) to chicken fetal antigen (cfa) expressed on the cell surface of the marek's disease lymphoblastoid cell line mdcc-msb1 was generated. 2h3 reacted specifically with various cells from chicken embryos, especially with fetal red blood cells, fetal bursa-derived lymphocytes, fetal liver cells, and embryo fibroblasts but not with fetal peripheral blood lymphocytes. 2h3 reacted also with all lymphoblastoid cell line cells tested: marek's disease, avian leukosis, and reticul ... | 1986 | 2428485 |
| methylation of marek's disease virus dna in chicken t-lymphoblastoid cell lines. | methylation of marek's disease virus serotype 1 (mdv1) dna in both productively and latently infected cells was examined by restriction endonuclease analyses with the isoschizomeric pair hpaii and mspi. the latent mdv1 dna in t-lymphoblastoid cell lines established from chicken t-lymphomas was considerably methylated, whereas methylation of the virus dna sequences was not detected in productively infected cells. | 1987 | 2437246 |
| copy number of tandem direct repeats within the inverted repeats of marek's disease virus dna. | we previously reported that dna of the oncogenic strain bc-1 of marek's disease virus serotype 1 (mdv1) contains three units of tandem direct repeats with 132 base pair (bp) repeats within the inverted repeats of the long regions of the mdv1 genome, whereas the attenuated, nononcogenic viral dna contains multiple units of tandem direct repeats (maotani et al., 1986). in the present study, the difference in the copy numbers of 132 bp repeats of oncogenic and nononcogenic mdv1 dnas in other strain ... | 1986 | 2441693 |
| maintenance of marek's disease herpesvirus latency in vitro by a factor found in conditioned medium. | chicken spleen cells latently infected with marek's disease virus were cultured with and without conditioned medium (cm) obtained from concanavalin a-stimulated chicken spleen cell cultures. the expression of viral internal antigen(s) (via), which is usually associated with cultivation, was prevented or markedly reduced by the cm. this effect required the continued presence of cm, since its removal after 48 h resulted in the subsequent appearance of via. although cm contains both gamma interfero ... | 1988 | 2460584 |
| in situ production of interferon in tissues of chickens exposed as embryos to turkey herpesvirus and marek's disease virus. | chicken eggs at embryonation day (ed) 18 or newly hatched chicks were inoculated with turkey herpesvirus (hvt), marek's disease virus (mdv), or virus-free diluent and, at intervals after inoculation, tissue homogenates of virus-exposed and virus-free chickens or chicken embryos were examined for interferon (ifn) activity. homogenates of lung, thymus and spleen specimens from chickens given hvt at ed 18 had ifn activity. activity of ifn in the lungs was studied further. homogenates of lung specim ... | 1989 | 2475045 |
| induction of cell mediated immune response by nonspecific stimulator of immunity against antigenically unrelated oncogenic virus (marek's disease virus). | an enzyme treated preparation of mycobacterium phlei (nsi), induced strong cell mediated immune response (cmir) against specific as well as against nonspecific oncogenic marek's disease (mdv) in birds, as evinced by lymphocyte migration inhibition, (lmit) lymphocyte transformation test (lt) and lymphokine (lymphocyte migration inhibition factor) lyif assay. maximum cmir could be observed towards third week post inoculation. all the three tests exhibited a positive correlation. such phenomenon of ... | 1989 | 2479599 |
| augmentation of retrovirus-induced lymphoid leukosis by marek's disease herpesviruses in white leghorn chickens. | our objective was to determine whether the cell-associated herpesvirus vaccines used in chickens to control marek's disease tumors can augment development of lymphoid leukosis (ll) induced by exogenous avian leukosis virus (alv). various single or mixed marek's disease vaccines were inoculated at day 1, and alv was injected at 1 to 10 days, with chickens of several experimental or commercial strains. development of ll was monitored at 16 to 48 weeks in various experiments. in several strains of ... | 1989 | 2536088 |
| discovery of noninfectious viral genes complementary to marek's disease herpes virus in quail susceptible to cholesterol-induced atherosclerosis. | japanese quail genetically selected on the basis of atherosclerosis susceptibility were tested for infection by marek's disease herpesvirus (mdv). viral dna was detected in the atherosclerotic aortas of susceptible (sus) quail by the technique of dna hybridization. southern blot analysis demonstrated that restriction mapping of aortic dna was specific and different from that of mdv. screening of quail embryos by dot-blot hybridization detected that mdv dna existed in 100% of sus quail tested. re ... | 1989 | 2537395 |
| survey of vaccines and spf flocks for contamination with chick anaemia agent. | 1989 | 2538021 | |
| influence of thymic hormone on cell-mediated and humoral immune responses in marek's disease. | a reduction in the secretion of thymic hormones, and in particular thymulin, can be demonstrated in chickens following the thymic atrophy induced by marek's disease virus (mdv) infection. in very sensitive histocompatible (b13/b13) chickens inoculated with the hprs-16 strain of mdv at 10 days of age, treatment with synthetic thymulin by daily subcutaneous injection failed to modify the time course of marek's disease (md) and did not prevent the development of macroscopic tumors. no effect was no ... | 1989 | 2538023 |
| immunohistological identification of both infectious bursal and marek virus antigens in the bursa of fabricius. | indirect immunoperoxidase (iip) and avidin biotin-peroxidase complex (abc) techniques were used for the detection of infectious bursal virus (ibv) and marek disease virus (mdv) antigens in alcohol and formalin-fixed, paraffin-embedded lymphoid tissues from broilers. both techniques appeared potentially useful for the diagnosis of both viral antigens in alcohol-fixed tissues, and allowed the observation of dual infection in the bursa of fabricius of the studied animals in a natural infection. | 1989 | 2540946 |
| structure of the marek's disease virus bamhi-h gene family: genes of putative importance for tumor induction. | the bamhi h fragment of marek's disease virus (mdv), which has been hypothesized to contain a tumor-inducing gene, was partially sequenced and analyzed for transcriptional activity. transcription of several rnas was found to initiate within bamhi-h, with the major transcripts initiating on either side of a putative mdv origin of lytic replication. a 1.8-kilobase gene family found to be produced only by pathogenic mdv was detected, and its transcription map was produced. it is hypothesized that t ... | 1989 | 2542569 |
| partial relaxation of enzyme-product binding by refolding of the growing chain in autocatalytic rna replication. | 1989 | 2542802 | |
| nucleotide sequence of the marek's disease virus (mdv) rb-1b a antigen gene and the identification of the mdv a antigen as the herpes simplex virus-1 glycoprotein c homologue. | the a antigen gene from a very virulent strain of marek's disease virus, rb-1b, has been cloned and the nucleotide sequence determined. the predicted amino acid sequence showed 99% identity to that determined for the mdv ga a antigen (coussens and velicer, j. virol. 62, 2373-2379, 1988) over all but the carboxy-terminal region where the sequence diverged extensively. the divergence results from three nucleotide frameshifts in the reported sequence of the mdv ga gene which are not present in a cl ... | 1989 | 2543160 |
| nucleotide sequence and characterization of the marek's disease virus homologue of glycoprotein b of herpes simplex virus. | the marek's disease virus (mdv) homologue of the herpes simplex virus (hsv) gene encoding glycoprotein b (gb) has been identified within bamhi fragments i3 and k3 of the 'highly oncogenic' strain rb1b of mdv. the entire nucleotide sequence of the gene has been determined and its predicted amino acid sequence shown to share gross overall structural features with the gb genes of hsv, varicella-zoster virus (vzv) and other mammalian herpesviruses. in particular, all 10 cysteine residues were conser ... | 1989 | 2544666 |
| partial transcription map of marek's disease herpesvirus in lytically infected cells and lymphoblastoid cell lines. | marek's disease herpesvirus (mdv) can cause either a productive-restrictive or lytic infection, a latent infection or can transform thymus-derived lymphocytes. rna was extracted from infected chicken embryo fibroblasts (cef) or from lymphoblastoid tumour cell lines. some of the infected cef were treated with 200 micrograms/ml cycloheximide to identify immediate early (ie) transcripts, and others with 1 microm 1-(2-fluoro-2-deoxy-b-d-arabinofuranosyl)-5-methyluracil (fmau), an inhibitor of herpes ... | 1989 | 2545632 |
| pathogenesis of marek's disease virus-induced local lesions. 1. lesion characterization and cell line establishment. | subcutaneous (wing-web) or intramuscular inoculation of chickens with allogeneic normal or marek's disease virus (mdv)-infected chicken kidney cells induced local lesions visible by 3-4 days postinoculation (pi). lesions were slightly larger (p less than 0.05) in infected than uninfected chickens 5 and 8 days pi. they persisted and grew past 9 days pi only when infected. infiltrating lymphocytes in infected and uninfected early lesions were similar; they included b-cells and also t-cells with an ... | 1989 | 2546529 |
| the role of the reticuloendothelial system in the immunopathology of marek's disease. | the role of macrophages in immunity against marek's disease (md) was studied. chickens of one group were subjected to depletion of macrophages using repeated doses of francil amorphous silica and those of another group were subjected to activation of macrophages using repeated doses of brewer's thioglycollate broth. chickens of a third group were vaccinated with herpesvirus of turkeys fc 126 vaccine followed by depletion of macrophages. chickens of these three groups, as well as groups of health ... | 1989 | 2549684 |
| transplantable marek's disease lymphomas. iii. induction of mhc-restricted tumor immunity by lymphoblastoid cells in f1 hosts. | f1 chickens, which were a cross between birds of the related inbred lines g-b1 and g-b2, were immunized with in vitro cultured virus-non-producer lymphoblastoid cells that were either syngeneic or allogeneic with the challenge tumor cells. the lymphoblastoid cells were derived from marek's disease herpesvirus (mdv)-induced transplantable tumors. previous findings showed that such immunization of g-b1 and g-b2 chickens prevented early mortality caused by the tumors. lymphoblastoid cells syngeneic ... | 1989 | 2550373 |
| loss of marek's disease virus tumorigenicity is associated with truncation of rnas transcribed within bamhi-h. | the attenuation of marek's disease virus (mdv) is associated with loss of pathogenicity and tumorigenicity. previous studies have demonstrated a strong correlation between attenuation and amplification of a specific sequence located within the mdv terminal and internal repeats. we recently reported that the regions containing the amplified sequences, the bamhi d and h fragments, were transcriptionally active. however, differential transcription activity was observed to exist between attenuated a ... | 1989 | 2550661 |
| identification of virus-specific polypeptides by monoclonal antibodies against serotype 2 marek's disease virus. | a total of 41 antibody-secreting hybridoma cells against the hprs24 strain of marek's disease virus (mdv) type 2 (mdv2) have been isolated. of these monoclonal antibodies (mabs), 24 were found by immunofluorescence tests to react specifically with mdv2-infected cells, but not mdv type 1 (mdv1)- or herpesvirus of turkeys (hvt)-infected cells, while eight reacted with mdv1- or mdv2-infected cells and nine with mdv1-, mdv2- or hvt-infected cells. by using these mabs, seven classes of mdv type-speci ... | 1989 | 2552003 |
| studies on the pathology of marek's disease following challenge in chicks vaccinated with three different vaccines. | the pathology of marek's disease (md) was studied in chickens vaccinated separately with three different vaccines, namely herpesvirus of turkeys (hvt), inactivated marek's disease virus (imdv) and a bivalent vaccine. the md-specific mortality following challenge was significantly lower (2.0 per cent) in bivalent vaccinated birds than in those vaccinated with either hvt (5.9 per cent) or imdv (13.7 per cent) alone. the occurrence, extent and severity of gross lymphomas and lymphoproliferative les ... | 1989 | 2555401 |
| a comparative analysis of the sequence of the thymidine kinase gene of a gammaherpesvirus, herpesvirus saimiri. | we present the nucleotide sequence of a region from the genome of the a + t-rich gammaherpesvirus, herpesvirus saimiri (hvs), which includes the coding sequences for the viral thymidine kinase (tk) gene. the organization of genes in this region resembles the homologous region of the epstein-barr virus (ebv) genome and is very compact, using overlapping coding sequences and with nucleotides shared by initiation and termination codons of neighbouring reading frames. the hvs tk is predicted to cont ... | 1989 | 2555434 |
| nucleotide and predicted amino acid sequences of the marek's disease virus and turkey herpesvirus thymidine kinase genes; comparison with thymidine kinase genes of other herpesviruses. | in this paper we present the nucleotide sequences of the thymidine kinase (tk) genes of two avian herpesviruses: a highly oncogenic strain of marek's disease virus (mdv strain rb1b) and its serologically related vaccine virus, the herpesvirus of turkeys (hvt strain fc-126). the predicted coding regions of the two genes are 1029 and 1050 nucleotides respectively, corresponding to polypeptides of 343 and 350 amino acids in length. putative nucleotide- and nucleoside-binding sites have been identif ... | 1989 | 2555435 |
| enhanced pathogenicity of chicken anemia agent by infectious bursal disease virus relative to the occurrence of marek's disease vaccination breaks. | 1989 | 2555622 | |
| homologies between herpesvirus of turkey and marek's disease virus type-1 dnas within two co-linearly arranged open reading frames, one encoding glycoprotein a. | the genomes of two avian herpesviruses, marek's disease virus type 1 (mdv1) and herpesvirus of turkey (hvt), share close homology only within certain dna regions. one such homologous region of hvt dna was cloned and sequenced. two open reading frames (orfs) were found in the long unique region, orf1 encoding the glycoprotein a (ga), and orf2 encoding a still unidentified protein. these two hvt-orfs are located at almost the same positions as the homologous mdv1-orfs. the nucleotide sequence homo ... | 1989 | 2558972 |
| comparison of the sequence of the secretory glycoprotein a (ga) gene in md5 and bc-1 strains of marek's disease virus type 1. | dna fragments containing the secretory glycoprotein a (ga) gene of marek's disease virus type 1 (mdv1) were cloned from the dna libraries of very virulent md5 and virulent bc-1 strains and sequenced. two open reading frames (orf1 and orf2) were identified for both strains. the orf1 has the potential to code for a protein of 501 amino acids with a molecular weight of 56 kd that contains strong hydrophobic regions in both the amino and carboxyl termini, and nine potential n-linked glycosylation si ... | 1989 | 2559540 |
| practical application of nucleic acid techniques to avian disease problems. | a workshop in which 17 practicing scientists participated was intended to address primarily people who use or could use biotechnology in their work and was confined to five techniques. endonuclease fingerprinting and mapping involved cleaving nucleic acid with a specific restriction enzyme and separating the nucleic acid fragments by electrophoresis. field and vaccine isolates of pasteurella multocida could be distinguished; salmonella enteritidis could be divided into three groups; chlamydia co ... | 1989 | 2559697 |
| a primary epidemic of inclusion body hepatitis in broilers. | inclusion body hepatitis (ibh) was diagnosed in 15 broiler flocks supplied by one breeder in the south island of new zealand. the affected flocks suffered mortality up to 30%. malaise and slightly increased mortality were noticed by growers from about day 12 post-hatch; mortality peaked in the fourth week, and, in most flocks, declined to normally accepted levels from day 33 on. gross signs seen at necropsy usually included bone-marrow aplasia, atrophy of the bursa of fabricius and the thymus, a ... | 1989 | 2559699 |
| in situ detection by monoclonal antibody d-35.1 of cells infected with marek's disease virus that interact with splenic ellipsoid-associated reticulum cells. | immuno- and enzyme-histochemical staining procedures were used to investigate in vivo the interaction of marek's disease virus (mdv) with splenic non-lymphoid cells. the newly developed monoclonal antibody d-35.1, which recognizes all three mdv serotypes, was used to study the localization of mdv at various times after intramuscular inoculation of 1-day-old chicks with mdv strain k. the d-35.1-positive cells were detected in the bursa of fabricius, spleen, thymus, proventriculus, and cecal tonsi ... | 1989 | 2559702 |
| transient depletion of cortical thymocytes induced by chicken anaemia agent. | chicken anaemia agent (caa) causes severe anaemia, loss of body weight, and hypoplasia of thymus at day 14 after inoculation of one-day-old chickens. several reports have described an enhancement of concurrent infections with f.e. marek's disease virus, infectious bursal disease virus, and reovirus. immunohistochemical methods were used to describe the immunopathological lesions of the thymus that probably form the basis of the immunodeficiency caused by caa. monoclonal antibodies and antisera a ... | 1989 | 2560270 |
| marek's disease virus-induced transient paralysis in chickens: demonstration of vasogenic brain oedema by an immunohistochemical method. | the presence of vasogenic brain oedema and its distribution in marek's disease virus (mdv)-induced transient paralysis (tp) were determined in genetically resistant and susceptible inbred white leghorn chickens. mdv-inoculated tp-susceptible chickens with nervous signs (9 days post-inoculation) had severe vacuolation of cerebellar white matter and associated diffuse leakage of albumin and igg. the serum protein leakage was associated morphologically with a vasculitis and intramural pseudocyst fo ... | 1989 | 2607017 |
| [karyometric differences in the tissue of lymphoid organs in marek's disease and lymphoid leukosis in poultry]. | histological preparations from the bursa of fabricius and the thymus of chickens of egg layer hx-sl were examined caryometrically by the picture analyser microvideomat 1. the chickens were allocated in tens to 3 groups (healthy, infected with marek's disease virus and infected with avian leucosis virus). the surface areas and perimeters of the nuclei were measured and the form factor calculated. the basic statistical parameters for each sample were calculated and consequently the three previousl ... | 1989 | 2609479 |
| [use of nitrovin in feed and its effect on the development of marek's disease in chickens]. | the effect of the czechoslovak product nitrovin, administered at a rate of 15 mg per kg of feed mixture, was studied as exerted on the growth activity, meat quality, and the development and intensity of occurrence of patho-morphological changes ascribable to marek's disease. an increase of weight gains and dressing percentage was recorded in the group of birds given nitrovin, as well as in those which had nitrovin in their feed and were infected with a highly virulent virus of marek's disease. n ... | 1989 | 2631377 |
| distribution and function of non-lymphoid cells positive for monoclonal antibody cvi-chnl-68.2 in healthy chickens and those infected with marek's disease virus. | immuno-enzyme histochemistry was used to study the staining pattern and tissue distribution of monoclonal antibody cvi-chnl-68.2 that specifically reacts with a subset of non-lymphoid cells in healthy chickens and those infected with marek's disease virus (mdv). functional characteristics of cvi-chnl-68.2-positive cells, e.g. antigen uptake, are determined. in the liver cvi-chnl-68.2 recognizes reticulum cells, whereas in the bursa of fabricius it detects single cells in the interfollicular conn ... | 1989 | 2683357 |
| immune response versus susceptibility to marek's disease. | it was hypothesized that the generation of activated t cells through an efficient and rapid immune response during the early pathogenesis of marek's disease virus (mdv) infection provides a large pool of target cells for transformation. therefore, the correlation between genetic susceptibility to marek's disease (md) and in vitro mitogenic responses of lymphocytes as a measure of cell-mediated immune competence and efficiency was tested. in one series of trials, spleen cells from strains of chic ... | 1989 | 2784854 |
| eimeria tenella and e. acervulina: lymphokines secreted by an avian t cell lymphoma or by sporozoite-stimulated immune t lymphocytes protect chickens against avian coccidiosis. | the role of avian lymphokines as nonspecific immunomodulators of host immunity against the intracellular parasite eimeria was investigated. prophylactic treatment of normal chickens with crude cell-free supernatants obtained from jmv-1 culture, concanavalin a (con a)-stimulated normal spleen cells, or sporozoite-stimulated immune t cells prior to inoculation with e. tenella or e. acervulina conferred significant protection. these crude cell-free culture supernatants also inhibited intracellular ... | 1989 | 2786476 |
| characterization of a highly transcribed dna region of herpesvirus of turkeys. | a highly transcribed dna region of turkey herpesvirus (hvt) codes for multiple species of rna. although the rna is transcribed from both strands of dna, no overlapping complementary transcripts have been detected. the complete nucleotide sequence corresponding to the cdna of one of the hvt transcripts was determined. this sequence is highly homologous to the nucleotide sequence of marek's disease virus antigen gp57-65-coding gene and appears to represent the corresponding hvt-coding gene. | 1989 | 2792768 |
| efficacy of simultaneous administration of marek's disease and viral tenosynovitis vaccines to day-old broiler chickens. | experiments were conducted to determine the efficacy of simultaneous administration at 1 day of age of the herpes virus of turkeys vaccine (hvt) and viral tenosynovitis (vt) vaccine. day-old broilers from commercial breeders, which were immunized against both md and vt, were used. the vaccines were injected at full dosage or diluted 1 to 4. challenge with either marek's disease virus (mdv; intraperitoneal) or vt virus (footpad) was at 7 days of age. at 7 wk of age, birds were weighed, killed, an ... | 1989 | 2813247 |
| delayed replication of marek's disease virus following in ovo inoculation during late stages of embryonal development. | several oncogenic and non-oncogenic isolates of marek's disease virus (mdv) were inoculated into embryonated eggs on embryonation day (ed) 16 to 18, and embryos or chicks hatching from inoculated eggs were examined for infectious virus and viral internal antigen (via) in lymphoid organs. there was no evidence of extensive replication of mdv in any of the embryonic tissues examined. levels of via peaked 4-5 days after chicks hatched. this indicated that mdv remained inactive during embryonation a ... | 1987 | 2823773 |
| marek's disease: a model for protection against herpesvirus-induced tumours. | marek's disease (md) is a lymphoproliferative disease of chickens caused by a herpesvirus (mdv). several effective vaccines have been developed and md is therefore often considered as a model for studying antitumour vaccines. key factors for the understanding of vaccine-induced immunity are discussed. three serotypes have been characterized: serotype 1 or oncogenic mdv, serotype 2 or non-oncogenic mdv and serotype 3 or herpesvirus of turkeys. the three serotypes have clearly different genomes an ... | 1987 | 2825984 |
| biological characteristics of marek's disease vaccine cvi-988 clone c1. | biological characteristics of marek's disease virus (mdv) cvi-988 clone c, of importance for vaccine application, are described. cvi-988 clone c was shown to be nonpathogenic for highly md-susceptible chickens and slightly more effective than prototype cvi-988 vaccine. during plaque purification and serial cell-culture passages, reductions were observed in the release of 'a' antigen from infected cell cultures, in spreading properties and in virus replication in vivo. pre-licensing batches of cv ... | 1987 | 2829408 |
| [the riems marek disease vaccine--a substantial contribution to the protection of industrialized poultry production]. | 1987 | 2829765 | |
| a gene for dihydrofolate reductase in a herpesvirus. | the enzyme dihydrofolate reductase (dhfr) is found ubiquitously in both prokaryotes and eukaryotes. it is essential for de novo synthesis of purines and of deoxythymidine monophosphate for dna synthesis. among viruses, however, only the t-even and t5 bacteriophage have been found to encode their own dhfr. in this study a gene for dhfr was found in a specific subgroup of the gamma or lymphotropic class of herpesviruses. dna sequences for dhfr were found in herpesvirus saimiri and herpesvirus atel ... | 1988 | 2830673 |
| marek's disease. xvii. studies on virus induced-atherosclerosis. | marek's disease herpesvirus (mdv) specific internal antigens were found in arterial segments of single comb white leghorn, the cornell strain, and of broiler chickens infected with virulent type 1 mh-87 strain of mdv. atherosclerosis was reproducibly induced in specific pathogen free (spf) and in broiler chickens by infection with mh-87 strain of mdv. under these conditions a direct association was established between mdv infection and mdv-induced-atherosclerosis. | 1987 | 2830714 |
| reduced shedding of marek's disease virus from feather follicles of bivalent vaccinated chickens. | 1987 | 2831141 | |
| [tumor disease and mortality without lymphomas following infection by marek's disease virus in highly and slightly susceptible chickens]. | 1987 | 2831831 | |
| [procedure for assessing the biologic suitability of dimethyl sulfoxide as a cryoprotective agent in preserving virus-infected primary poultry cells]. | 1987 | 2831833 | |
| avian adeno-associated parvovirus and marek's disease virus: studies of viral interactions in chicken embryo fibroblasts. brief report. | using growth kinetics we demonstrate two effects based on interactions between the chicken herpesvirus, marek's disease virus (mdv), and the dependovirus, avian adeno-associated virus (aaav), in coinfected chicken embryo fibroblasts (cef): (i) mdv provides helper activity for an efficient multiplication of aaav; (ii) a high multiplicity of coinfecting aaav inhibits completely the growth of mdv as well as aaav. | 1988 | 2831859 |
| new serotype 2 and attenuated serotype 1 marek's disease vaccine viruses: comparative efficacy. | attempts were made, through selection of optimum viral strains, to develop improved vaccines against marek's disease (md). seven attenuated serotype 1 strains and 22 avirulent serotype 2 strains, both alone and in combination with the fc126 strain of serotype 3, were screened for protective efficacy against challenge with virulent and very virulent md viral strains. the three viruses selected as most promising were evaluated alone and in various combinations and compared with commercially availa ... | 1987 | 2831867 |
| new serotype 2 and attenuated serotype 1 marek's disease vaccine viruses: selected biological and molecular characteristics. | two new marek's disease vaccine viruses, md11/75c/r2 (serotype 1) and 301b/1 (serotype 2), were evaluated in chickens with maternal antibodies (ab+) or without maternal antibodies (ab-). strain md11/75c/r2 was mildly pathogenic in ab--chickens, but this pathogenicity was markedly reduced in ab+ chickens. md11/75c/r2 spread less by contact and replicated better, both in vivo and in vitro, than cvi988/c, another serotype 1 vaccine virus. strain 301b/1 was similar to sb-1, another serotype 2 vaccin ... | 1987 | 2831870 |
| effect of immunocompetence on the establishment and maintenance of latency with marek's disease herpesvirus. | marek's disease virus (mdv) infections normally have an early cytolytic phase in lymphoid organs at 3 to 6 days post-infection followed by a period of latent infection. little is known about the mechanisms that govern latency with herpesvirus infections, including marek's disease (md). to investigate the importance of immunocompetence for either the establishment or the maintenance of latency in md, immunosuppressive treatments were applied prior to infection with mdv or after latency was establ ... | 1988 | 2836551 |
| structure and complete nucleotide sequence of the marek's disease herpesvirus gp57-65 gene. | the gene encoding the precursor polypeptide of the marek's disease herpesvirus (mdhv) secretory glycoprotein gp57-65 (formerly identified as a antigen) has been sequenced. previous results had localized the gene to a 4.6-kilobase (kb) segment of the bamhi b fragment in the unique long region of the mdhv genome. s1 nuclease protection experiments were used to more precisely locate the 5' initiation and approximate 3' termination points of the approximately 1.8-kb mdhv gp57-65 mrna within this seg ... | 1988 | 2836620 |
| turkey herpesvirus. vii. the evolution of marek's disease in chickens vaccinated at one day of age and challenged at different periods of time after vaccination. | the single comb white leghorn, the cornell s strain chickens vaccinated at one day of age with cell free herpesvirus of turkey (hvt) 100 and 1000 focus forming-units (ffu) per bird were raised in isolatores. at different periods of time the vaccinated and control chickens were reared together with chickens with clinical symptoms of marek's disease previously inoculated with jm-strain of marek's disease virus (mdv) in a separate isolation room throughout the experiment. the specific protective ef ... | 1988 | 2837003 |
| indications of immunodepression in chickens infected with various strains of marek's disease virus. | the effect of infection by various strains of marek's disease virus (mdv) on the immune function of 3-week-old chickens was examined. mdv strains of low (cu-2, rb-7) and high (rb-3, md-5, and md-11) pathogenicity were compared with prototype jm-10 strain of moderate pathogenicity. mortality, whole body weight, relative weights of lymphoid organs, histopathology, humoral antibody responses to thymus-dependent and -independent antigens, and in vitro lymphocyte responses to mitogen stimulation were ... | 1988 | 2838012 |
| the structure of marek's disease virus dna: amplification of repeat sequence in irs and trs. | the structure of marek's disease virus (mdv) dna was investigated by using southern blot hybridization analysis. a heterogeneous region was observed in the inverted repeats region, irs and trs, as well as in the trl and irl. the results of dna sequencing of the heterogeneous region showed that the heterogeneity of irs and trs was due to amplification of a 178-bp repeat sequence. amplification of irs and trs was found in viral dna from both pathogenic and nonpathogenic strains. the structure of d ... | 1988 | 2839751 |
| gene sequence and mapping data from marek's disease virus and herpesvirus of turkeys: implications for herpesvirus classification. | purified dnas from marek's disease virus (mdv) and the herpesvirus of turkeys (hvt) were randomly sheared and cloned into the m13 bacteriophage. two-hundred and ten mdv and 130 hvt clones were sequenced to give representative samples of the genome sequences. the predicted amino acid sequences from these gammaherpes-viruses were compared to known sequences from other herpesviruses using computer analysis. thirty-five mdv and 24 hvt genes were identified by comparison with varicella-zoster virus ( ... | 1988 | 2841415 |
| identification of marek's disease virus nuclear antigen in latently infected lymphoblastoid cells. | a new marek's disease virus (mdv) nuclear antigen (mdna) was identified in two mdv-transformed t-lymphoblastoid cell lines, mkt-1 and msb-1, derived from chickens bearing tumors induced by mdv. this mdna was not detected in msb-1 cells maintained in iododeoxyuridine, which activates the latent mdv genome. moreover, it was not found in chicken embryo fibroblasts undergoing productive and cytolytic infection with mdv. expression of mdna is not related to strain pathogenicity in chickens, because c ... | 1988 | 2843674 |
| synthesis and processing of the marek's disease herpesvirus b antigen glycoprotein complex. | the marek's disease herpesvirus b antigen (mdhv-b) complex was previously immunologically identified and molecularly characterized as a set of three glycoproteins designated gp100, gp60, and gp49 on the basis of apparent molecular weight and immunoprecipitation with both polyclonal and monoclonal antibodies. immunoprecipitation analysis, previously with polyclonal and more recently with monoclonal antibodies, of infected cell lysates labeled with [35s]methionine in the presence of tunicamycin, a ... | 1988 | 2845139 |
| presence of natural suppressor cells in the chicken embryo spleen and the effect of virus infection of the embryo on suppressor cell activity. | spleen cells but not the thymus or the bursa cells of chicken embryos suppressed the in vitro mitogenesis of spleen cells of adult syngeneic or allogeneic chickens. the natural suppressor cell activity of embryo spleen was present at embryonation day 16, reached peak levels at embryonation day 18 and disappeared at hatch. the embryo spleen cells did not by themselves respond to phytohemagglutinin stimulation in vitro. the suppressive effect of embryonic spleen cells on adult spleen cells was pre ... | 1988 | 2845637 |
| a repeat sequence, gggtta, is shared by dna of human herpesvirus 6 and marek's disease virus. | some regions of the genomes of human b-lymphotrophic virus (hblv), also designated as human herpesvirus 6, and marek's disease virus were found to hybridize to each other under moderate to stringent conditions, scoring from 10 to 30% base-pair mismatch. nucleotide sequence analysis showed that a 6-base-pair repetitive sequence, gggtta (dr2), present in the irs-irl junction region of the marek's disease virus genome, was also reiterated in the hblv genome. the function(s) of such a sequence is un ... | 1988 | 2846894 |
| [effect of a mixed viral infection on the development of neoplasms induced by oncogenic viruses]. | data are presented concerning the stimulating effect of vaccinia and herpes simplex type 2 viruses on the development of leukemia in balb/c, c57bl/6, and akr mice. mixed infection with pab-49 and marek disease virus of brown leghorn chickens was shown to increase the frequency of lymphomas development. | 1988 | 2848364 |
| immune response of chicks inoculated with chicken anemia agent alone or in combination with marek's disease virus or turkey herpesvirus. | 1988 | 2848964 | |
| some aspects of lymphomagenesis of marek's disease of chickens. | 1988 | 2848997 | |
| [isolation of the pathogenic virus of marek's disease from a flock of chickens vaccinated with the turkey herpes vaccine]. | 1988 | 2851887 | |
| rna transcripts of marek's disease virus (mdv) serotype-1 in infected and transformed cells. | rna was isolated from two strains of marek's disease virus (mdv-z and mdv-b). the virus was grown in duck embryo fibroblasts (def) for 96 hr, 72 hr in the presence of phosphonoacetic acid (paa) and 24 hr in the presence of cycloheximide added at the time of infection. with the use of dna probes representing about 80% of the mdv genome, an extensive northern blot analysis of the rna was carried out. a similar analysis was done with rna extracted from the mdv-transformed cell line msb-1. this stud ... | 1988 | 2852415 |
| marek's disease virus serotype-1 antigens a and b and their unglycosylated precursors detected by western blot analysis of infected cells. | the antigenic profile of cell cultures infected with marek's disease virus (mdv) was determined by the immunoblotting method using convalescent immune serum obtained from chickens that survived infection with mdv strain ga5. the mdv antigen profile in infected cell lysates could be accurately determined since this method has advantages over the immunoprecipitation method used in other studies. we studied six very virulent mdv isolates and the prototype of serotype 1 mdv, the ga5 strain. immunobl ... | 1988 | 2852416 |
| virus-induced atherosclerosis. herpesvirus infection alters aortic cholesterol metabolism and accumulation. | infection of normocholesterolemic, specific-pathogen-free chickens with marek's disease herpesvirus (mdv) has been shown histologically to lead to chronic atherosclerosis like that in humans. the development of herpesvirus-induced atherosclerosis in vivo and the presence of specific marek's antigen within aortic cells suggested that mdv infection may modify lipid metabolism and lead to significant lipid accumulation. experiments reported herein were designed to determine the types and quantity o ... | 1986 | 2934987 |
| identification of marek's disease virus-specific antigens in marek's disease lymphoblastoid cell lines using monoclonal antibody against virus-specific phosphorylated polypeptides. | for identification of the antigens specific to marek's disease virus (mdv) in virus-non-producer lymphoblastoid cell lines established from a tumor of marek's disease (md), hybridomas producing monoclonal antibodies (mabs) against the antigens were isolated. immunogens for preparation of the hybridomas were purified from the lysate of an md-lymphoblastoid cell line, msb1, by affinity chromatography coupled with chicken anti-mdv serum immunoglobulin g. three of the mabs obtained, mb1, mb2 and mb3 ... | 1985 | 2982746 |
| map location of homologous regions between marek's disease virus and herpesvirus of turkey and the absence of detectable homology in the putative tumor-inducing gene. | the dna region having homology between marek's disease virus and herpesvirus of turkey was assigned to the restriction map of marek's disease virus by southern blot hybridization. under moderate conditions at the level of 15% mismatching, homology was found to be distributed throughout the marek's disease virus genome. the long inverted-repeat regions (trl and irl), which are considered to play a significant role in tumorigenicity, did not show any homology to herpesvirus of turkey dna. | 1985 | 2983105 |
| the structure of marek disease virus dna: the presence of unique expansion in nonpathogenic viral dna. | dna of marek disease virus (mdv) consists of two unique regions ul and us flanked by long inverted repeat regions trl and irl, and short inverted repeat regions trs and irs, respectively, similar to herpes simplex virus dna. comparison of restriction patterns between pathogenic and nonpathogenic mdv dna was made to identify a region of viral dna different between these two types of mdv, as it may be responsible for the tumorigenicity of mdv in chickens. the results indicated that bamhi-d and -h, ... | 1985 | 2983314 |
| effects of chemically or virus-induced immunodepression on response of chickens to avian leukosis virus. | the effects of chemically or virus-induced immunodepression on the infection profile (development of viremia and antibody) and shedding of avian leukosis virus (alv) were studied in progeny chickens of experimental or commercial breeder flocks. chickens were infected with alv subgroup a by contact at hatching and by oral inoculation at 4-5 weeks of age. in the first experiment, chickens were inoculated with a virulent strain of infectious bursal disease virus (ibdv) at 1 day or 6 weeks of age. i ... | 1985 | 2985037 |
| gel electrophoretic analysis of polypeptides from nucleocapsids of marek's disease virus strains and herpesvirus of turkey. | the polypeptides of nucleocapsids of marek's disease virus (mdv) strains with different biological properties and of antigenically related herpesvirus of turkey (hvt) strains were analysed by one- and two-dimensional (1d and 2d, respectively) gel electrophoresis. based on small differences in migration behaviour (size and charge) of a number of corresponding nucleocapsid polypeptides, the virus strains could be differentiated into three groups. the polypeptide pattern of group i, comprising the ... | 1985 | 2986577 |
| differences in the processing of secreted glycoprotein a induced by marek's disease virus and herpesvirus of turkeys. | the steps from the precursor to the processed forms of a secreted glycoprotein (ga) of marek's disease virus (mdv) and of herpesvirus of turkeys (hvt) were examined by two-dimensional gel electrophoresis of immunoprecipitates from the cell lysate and medium of infected cultures with monoclonal antibodies. differences between mdv- and hvt-ga were particularly observed in isoelectric points of the glycosylated or unglycosylated precursor forms. | 1985 | 2987399 |
| genomic expansion of marek's disease virus dna is associated with serial in vitro passage. | an ecori restriction endonuclease pattern of md11 virus dna, a very virulent strain of marek's disease virus (mdv), was obtained by using total cellular dna from infected cells. with the ecori restriction endonuclease pattern and a published bamhi map of mdv (fukuchi et al., j. virol. 51:102-109), we constructed a partial ecori map of a series of mdv clones (gift from h. j. kung). the clones were used to identify a region of the md11 genome which is altered as the oncogenic virus is passaged in ... | 1985 | 2987523 |
| on the specificity of the immunoglobulin g produced by chickens experimentally infected with marek's disease virus. | in chickens experimentally infected with marek's disease virus (mdv) an increased amount of immunoglobulin g is produced. using a technique of quantitative crossed immunoelectrophoresis it has been shown, that 70% of this immunoglobulin g is non-specific. only 18% could be absorbed with mdv strain cprl vii-infected chicken kidney cells, and only 5% with mdv-induced lymphoblastoid cells of the mdcc-msb1 cell line. it is hypothesized that the production the unspecific immunoglobulin g is caused by ... | 1985 | 2993996 |