Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
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| inheritance of species differences in female receptivity and song requirement between drosophila virilis and d. montana. | females of two drosophila virilis group species, d. virilis and d. montana, have different requirements for the courting males. in the present study we have examined species differences in female receptivity and male courtship song requirement using females' acceptance signal instead of copulation for measuring female readiness to mate. behavior of d. virilis and d. montana females and f1 and backcross hybrid females was observed in a single-pair courtships with d. virilis and d. montana males a ... | 1999 | 10783530 |
| expression and translocation of drosophila nuclear encoded cytochrome b(5) proteins to mitochondria. | dna sequence studies of cytochrome b(5) (cyt-b) genes from drosophila melanogaster and drosophila virilis predict that the drosophila cyt-b proteins are extremely hydrophobic and have at least eight potential transmembrane spanning domains. primary protein sequence analysis also predicts that the cyt-b proteins have mitochondrial targeting sequences and they contain sites for potential post-translational modification similar to other cytochrome proteins. we report the characterization of the cyt ... | 2000 | 10899459 |
| evolution of gypsy endogenous retrovirus in the drosophila obscura species group. | the ty3/gypsy family of retroelements is closely related to retroviruses, and some of their members have an open reading frame resembling the retroviral gene env. sequences homologous to the gypsy element from drosophila melanogaster are widely distributed among drosophila species. in this work, we report a phylogenetic study based mainly on the analysis of the 5' region of the env gene from several species of the obscura group, and also from sequences already reported of d. melanogaster, drosop ... | 2000 | 10908638 |
| origin and evolution of the regulatory gene male-specific lethal-3. | dosage compensation in drosophila is mediated by genes known as "male-specific lethals" (msls). several msls, including male-specific lethal-3 (msl-3), encode proteins of unknown function. we cloned the drosophila virilis msl-3 gene. using the information provided by the sequences of the drosophila melanogaster and d. virilis genes, we found that sequences of other species can be aligned along their entire lengths with msl-3. among them, there are genes in yeasts (the schizosaccharomyces pombe a ... | 2000 | 10908644 |
| [the heat-shock reaction is disturbed in a drosophila virilis strain incapable of a neurohormonal stress reaction]. | cellular stress response was investigated in two lines of d. virilis: wild type and line with disturbed neurohormonal stress-reaction. analysis of proteins, synthesized in salivary glands of larvae of both lines under heat stress, revealed malfunction in heat shock reaction of mutant specimen. this malfunction expresses in decreased level of heat shock protein synthesis. analysis of electrophoretic spectra of proteins from homogenates of imagoes of both lines maintained under normal conditions a ... | 2008 | 10920860 |
| evidence for selection at the fused locus of drosophila virilis. | the genomic dna sequence of a 2.4-kb region of the x-linked developmental gene fused was determined in 15 drosophila virilis strains. one common replacement polymorphism is observed, where a negatively charged aspartic amino acid is replaced by the noncharged amino acid alanine. this replacement variant is located within the serine/threonine kinase domain of the fused gene and is present in approximately 50% of the sequences in our sample. significant linkage disequilibrium is detected around th ... | 2000 | 10924468 |
| invasion of drosophila virilis by the penelope transposable element. | the penelope family of transposable elements (tes) is broadly distributed in most species of the virilis species group of drosophila. this element plays a pivotal role in hybrid dysgenesis in drosophila virilis, in which at least four additional te families are also activated. here we present evidence that the penelope family of elements has recently invaded d. virilis. this evidence includes: (1) a patchy geographical distribution, (2) genomic locations mainly restricted to euchromatic chromoso ... | 2000 | 11007494 |
| mobile elements and chromosomal evolution in the virilis group of drosophila. | species of the virilis group of drosophila differ by multiple inversions and chromosome fusions that probably accompanied, or led to, speciation. drosophila virilis has the primitive karyotype for the group, and natural populations are exceptional in having no chromosomal polymorphisms. we report that the genomic locations of penelope and ulysses transposons are nonrandomly distributed in 12 strains of d. virilis. furthermore, penelope and ulysses insertion sites in d. virilis show a statistical ... | 2000 | 11016976 |
| the dna replication-related element (dre)-dre-binding factor (dref) system may be involved in the expression of the drosophila melanogaster tbp gene. | the tata box binding protein (tbp) is a general transcription factor required for initiation by all three eukaryotic rna polymerases. previously, we found that the promoter region of the drosophila melanogaster tbp gene contains three sequences similar to the dna replication-related element (dre) (5'-tatcgata). in the present study, we found that the dre-like sequences are also present in the promoter of the drosophila virilis tbp gene, suggesting a role for these sequences in tbp expression. ba ... | 2000 | 11033359 |
| [genetic characteristics of the wing mutation odd(22) and its interaction with alleles of the delta locus in drosophila virilis]. | the dominant sex-linked semilethal mutation odd22 was isolated from progeny of a dysgenic cross of drosophila virilis lines. flies homozygous, heterozygous, and hemizygous for odd22 displayed multiple wing defects, including enlargements and gaps on the veins; irregularly thickened, branched, shortened, or completely reduced veins; and cuts on the wing margin. the most remarkable feature of the odd22 expression was a combination of both an increase and a reduction of the wing vein material simul ... | 2000 | 11033775 |
| [comparison of the structure and embryonal expression of the delta locus in drosophila virilis and drosophila melanogaster]. | 2011 | 11033804 | |
| drosophila virilis has long and highly polymorphic microsatellites. | comparative genomics is a powerful approach to inference of the dynamics of genome evolution. most information about the evolution of microsatellites in the genus drosophila has been obtained from drosophila melanogaster. for comparison, we collected microsatellite data for the distantly related species drosophila virilis. screening about 0.5 mb of nonredundant genomic sequence from genbank, we identified 239 dinucleotide microsatellites. on average, d. virilis dinucleotides were significantly l ... | 2000 | 11070052 |
| a telomeric satellite in drosophila virilis and its sibling species. | telomere elongation by telomerase is the most widespread mechanism among eukaryotes. however, alternative mechanisms such as homologous recombination between terminal satellite dnas are probably used in lower dipteran insects and in some plants. drosophila melanogaster uses the very unusual telomere elongation pathway of transposition of telomere-specific retrotransposable elements. the uniqueness of this telomere elongation mechanism raises the question of its origin. we, therefore, analyzed se ... | 2000 | 11072792 |
| genetic control of biogenic-amine systems in drosophila under normal and stress conditions. | the contents of octopamine and its precursors (tyrosine and tyramine) were studied in adults of two lines of drosophila virilis with contrasting stress responses. it was demonstrated that in individuals responding to stress by a hormonal stress reaction (line 101), the contents of octopamine and tyrosine are lower than in nonresponding flies (line 147). it was found that there is no difference between the lines in the level of tyramine under normal conditions. the dopamine response to stressor w ... | 2000 | 11091907 |
| control of drosophila paramyosin/miniparamyosin gene expression. differential regulatory mechanisms for muscle-specific transcription. | to define the transcriptional mechanisms contributing to stage- and tissue-specific expression of muscle genes, we performed transgenic analysis of drosophila paramyosin gene regulation. this gene has two promoters, one for paramyosin and one for miniparamyosin, which are active in partially overlapping domains. regions between -0.9 and -1.7 kilobases upstream of each initiation site contribute to the temporal and spatial expression patterns. by comparing the drosophila melanogaster and drosophi ... | 2001 | 11110792 |
| a versatile vector set for animal transgenesis. | genetic manipulation of a series of diverged arthropods is a highly desirable goal for a better understanding of developmental and evolutionary processes. a major obstacle so far has been the difficulty in obtaining marker genes that allow easy and reliable identification of transgenic animals. here, we present a versatile vector set for germline transformation based on the promiscuous transposons mariner, hermes and piggybac. into these vectors, we introduced a potentially universal marker syst ... | 2000 | 11151300 |
| comparative analysis of the nona region in drosophila identifies a highly diverged 5' gene that may constrain nona promoter evolution. | a genomic fragment from drosophila virilis that contained all the no-on-transienta (nona) coding information, plus several kilobases of upstream material, was identified. comparisons of nona sequences and the gene nona-like in d. melanogaster, a processed duplication of nona, suggest that it arose before the split between d. melanogaster and d. virilis. in both species, another gene that lies <350 bp upstream from the nona transcription starts, and that probably corresponds to the lethal gene l( ... | 2001 | 11156994 |
| dpkqdfmrfamide expression is similar in two distantly related drosophila species. | drosophila melanogaster dpkqdfmrfamide was isolated and its expression reported. distribution of dpkqdfmrfamide immunoreactivity is now described in drosophila virilis. dpkqdfmrfamide antibody stained a cell in the subesophageal ganglion in embryo. dpkqdfmrfamide antibody stained cells in the superior protocerebrum, subesophageal ganglion, thoracic ganglia, and an abdominal ganglion in larva, pupa, and adult. dpkqdfmrfamide antibody stained an additional pair of cells in the optic lobe and a cel ... | 2001 | 11179817 |
| evolutionary conservation of the leucine-rich repeat transmembrane protein gp150 in drosophila and bombyx. | gp150 is a transmembrane glycoprotein belonging to the leucine-rich repeat (lrr) superfamily. here we report the molecular characterization of a gp150 homolog in drosophila virilis, which is separated from drosophila melanogaster by about 60 million years. a silkworm bombyx mori gp150 homolog was identified through a sequence database search. sequence analysis revealed high conservation in the lrrs and cysteine motifs flanking the lrr region in the extracellular domain of gp150. using an in vivo ... | 2000 | 11180815 |
| biogenic amines in drosophila virilis under stress conditions. | the effect of heat stress (38 degrees c) on the content of dl-beta-(3,4-dihydroxyphenyl)alanine (dopa), dopamine, tyramine, octopamine, and their precursor tyr was studied in adults of two lines of drosophila virilis contrasting in their stress response. in individuals of line 101 responding to stress by a hormonal stress reaction, the contents of dopa, dopamine, octopamine, and tyr were lower than those of line 147 that did not respond to the stress. however, heat stress caused an increase in t ... | 2000 | 11210126 |
| ecdysteroids in stress responsive and nonresponsive drosophila virilis lines under stress conditions. | after exposure to thermal stress or a control temperature, the relative abundance of ecdysone (e) and 20-hydroxyecdysone (20e) was measured in a wild-type line of drosophila virilis (101) that is stress responsive and in a mutant line (147) that is not stress responsive. in line 101, the 20e content was higher and e content lower in females than in males. the abundance of e and 20e in females of line 147 was significantly higher than that in females of line 101. females of line 101 were found to ... | 2000 | 11210130 |
| [some regularities in the genetic control of the stress reaction in drosophila]. | the heat-shock response was studied in drosophila virilis strains with normal and impaired neurohormonal stress reaction. flies from the latter strain were shown to have the impaired heat-shock response. in this strain, transcription of the heat shock gene hsp83 is reduced and synthesis of all heat shock proteins is suppressed. the neurohormonal stress reaction (status of dopamine, octopamine, and juvenile hormone metabolic systems) was examined in d. melanogaster strains having normal and impai ... | 2001 | 11234428 |
| characterization of the hox cluster from the mosquito anopheles gambiae (diptera: culicidae). | the hox genes have been found to encode transcription factors, which specify the morphological identity of structures along the anteroposterior axis of animals ranging from worms to mice. the canonical set of nine genes is organized in a cluster in the genome of several protostomes and deuterostomes. however, within insects, whereas the hox genes are organized in a single cluster in the beetle tribolium castaneum, they are split into two separate groups in the flies drosophila melanogaster and d ... | 2000 | 11256376 |
| promoter analysis of the drosophila melanogaster gene encoding transcription elongation factor tfiis. | the promoter region of the drosophila melanogaster tfiis gene was characterized by transient expression assay. serial deletion analysis of the promoter region showed that the promoter region between -112 and +113 is required for the efficient expression of the d. melanogaster tfiis gene. the results also suggest that the dna fragments between -112 and -54 and between +94 and +113 contain the vital elements for the expression. the importance of these fragments was further substantiated by the fin ... | 2001 | 11311940 |
| identification of x chromosomal restriction fragment length polymorphism markers and their use in a gene localization study in drosophila virilis and d. littoralis. | we have identified six restriction fragment length polymorphism (rflp) markers based on unique gene sequences on the x chromosome of drosophila virilis and d. littoralis. the markers were localized by in situ hybridization on larval polytene chromosomes, and the conjugation of the x chromosomes of the two species was studied in salivary glands of interspecific hybrid female larvae. the gene arrangement of d. virilis and d. littoralis appeared to be very different at the proximal end of the x chr ... | 2001 | 11341735 |
| the structure and evolution of penelope in the virilis species group of drosophila: an ancient lineage of retroelements. | the penelope element is the key element responsible for mobilization of other transposable elements in the course of hybrid dysgenesis in drosophila virilis. penelope has an unusually complex, highly variable organization in all studied species of the virlis group. thc bridge1 element from the fish fugu rubripes is homologous to penelope, and database searches detected additional homologous sequences among expressed sequence tags from the flatworm schistosoma mansonii and the nematode ancylostom ... | 2001 | 11443348 |
| evolution of 5s rrna gene families in drosophila. | in drosophila virilis, the three clusters of 5s rrna genes on chromosome 5 comprise two different gene families (b and c), which differ profoundly in the organization of their spacer sequences. while c-type genes, which are found in two of the clusters, exhibit a true repetitive character, the b-type genes of the third cluster are each embedded in completely different genomic environments. southern blots of genomic dna of different d. virilis subspecies, d. hydei and d. melanogaster probed with ... | 2001 | 11448042 |
| fish retroposons related to the penelope element of drosophila virilis define a new group of retrotransposable elements. | poseidon and neptune are two ancient lineages of retroposons related to the penelope element from drosophila virilis. they have been identified in various teleost fish species, including the medakafish (oryzias latipes), and the pufferfishes fugu rubripes and tetraodon nigroviridis, whose genomes are currently being sequenced. some of these elements are highly reiterated in fish genomes. penelope-related elements were also identified in blood fluke, shrimp, sea urchin, cichlid fish and frog, sho ... | 2001 | 11459192 |
| ecdysone-controlled mrna stability in drosophila salivary glands: deadenylation-independent degradation of larval glue protein gene message during the larval/prepupal transition. | 20-hydroxyecdysone induces poly(a) shortening and the subsequent degradation of transcripts encoding the larval glue protein lgp-1 in drosophila virilis late third larval instar salivary glands. degradation concurs with the transient increase of ribonucleolytic activities in the gland cells. in vitro nuclease assays using crude cytoplasmic extracts of ecdysone-treated salivary glands demonstrate degradation to be deadenylation-independent and that the induced ribonucleolytic activities initiate ... | 2001 | 11500246 |
| the nona gene in drosophila conveys species-specific behavioral characteristics. | the molecular basis of species-specific differences in courtship behavior, a critical factor in preserving species boundaries, is poorly understood. genetic analysis of all but the most closely related species is usually impossible, given the inviability of hybrids. we have therefore applied interspecific transformation of a single candidate behavioral locus, no-on-transient a (nona), between drosophila virilis and d. melanogaster, to investigate whether nona, like the period gene, might encode ... | 2001 | 11514445 |
| characterization of muscle actin genes in drosophila virilis reveals significant molecular complexity in skeletal muscle types. | actin is a ubiquitous and highly conserved eukaryotic protein required for cell motility and locomotion. in this manuscript, we characterize the four muscle actin genes of the insect drosophila virilis and demonstrate strong similarities between the d. virilis genes and their homologues in drosophila melanogaster; intron locations are conserved, and there are few amino acid differences between homologues. we also found strong conservation in temporal expression patterns of the muscle actin genes ... | 2001 | 11520356 |
| the neuron-specific rna-binding protein elav regulates neuroglian alternative splicing in neurons and binds directly to its pre-mrna. | drosophila melanogaster neural-specific protein, elav, has been shown to regulate the neural-specific splicing of three genes: neuroglian (nrg), erect wing, and armadillo. alternative splicing of the nrg transcript involves alternative inclusion of a 3'-terminal exon. here, using a minigene reporter, we show that the nrg alternatively spliced intron (nasi) has all the determinants required to recreate proper neural-specific rna processing seen with the endogenous nrg transcript, including regula ... | 2001 | 11581160 |
| [the dopamine system stress reaction in adults of drosophila virilis is controlled by genes of chromosome 6]. | a linkage group of the gene responsible for changes of da titer under stress in adults of d. virilis was determined. line 160 of d. virilis, all autosomes of which bear visible recessive mutations, was used as an analyzer. flies of lines 160 and 147 were shown to differ in da content under normal conditions and in the way da metabolic system reacts to stress. among the offspring of the analyzing cross, groups of flies were found with one testable autosome from line 147 and all other chromosomes ... | 2013 | 11589047 |
| [structure and evolutionary role of the penelope mobile element in drosophila species of the virilis group]. | the mobile element penelope is activated and mobilizes several other transposons in dysgenic crosses in drosophila virilis. its structure proved to be complex and to vary greatly in all examined species of the virilis group. phylogenetic analysis of the reverse transcriptase (rt) domain assigned penelope to a new branch, rather than to any known family, of ltr-lacking retroelements. amino acid sequence analysis showed that the c-terminal domain of the penelope polyprotein is an active endonuclea ... | 2001 | 11605533 |
| [interaction of hormones in controlling reproductive function of drosophila females under stressful conditions is genetically determined]. | the effect of heat stress (38 degrees c) on the content of octopamine (oa) and 20-hydroxyecdysone (20he) was studied under normal and stressful conditions in adult flies of drosophila virilis lines contrasting in the level of the juvenile hormone (jh). the wild-type flies (line 101) exhibited a pronounced sex dimorphism for the content of both oa and 20he, which was substantially lower in this line than in flies of the mutant line 147. the level of both hormones increased in flies of line 101 ex ... | 2001 | 11642127 |
| promoter analysis of the drosophila melanogaster gene encoding the pterin 4alpha-carbinolamine dehydratase. | pterin-4alpha-carbinolamine dehydratase (pcd) is a key enzyme in the regeneration pathway of tetrahydrobiopterin. previously, we isolated and reported the drosophila melanogaster gene encoding pcd. in the present study, we isolated and characterized the drosophila virilis gene encoding pcd. the drosophila virilis pcd gene has two introns and an open reading frame to encode a protein of 101 amino acids. the amino acid sequence of drosophila virilis pcd shows a 83% homology to that of the drosophi ... | 2001 | 11710527 |
| drosophila mef2 is a direct regulator of actin57b transcription in cardiac, skeletal, and visceral muscle lineages. | to identify regulatory events occurring during myogenesis, we characterized the transcriptional regulation of a drosophila melanogaster actin gene, actin 57b. act57b transcription is first detected in visceral muscle precursors and is detectable in all embryonic muscles by the end of embryogenesis. through deletion analysis we identified a 595 bp promoter element that was sufficient for high levels of expression in all three muscle lineages. this fragment contained a mef2 binding site conserved ... | 2002 | 11744367 |
| nucleotide variation at the no-on-transient a gene in drosophila littoralis. | the no-on-transient a (nona) gene encodes a putative rna-binding protein, and mutations in this gene are known to affect vision, male courtship song and viability in drosophila melanogaster. here we have sequenced the coding region of the nona gene of drosophila littoralis and compared it with those of drosophila virilis and d. melanogaster. all portions of nona appeared to be conserved between d. littoralis and d. virilis, while the 5' region of the gene of these two species showed high diverge ... | 2002 | 11813105 |
| conservation of gene order, structure and sequence between three closely linked genes in drosophila melanogaster and drosophila virilis. | in drosophila melanogaster, the apparently unrelated genes anon-66da, rpl14, and anon-66db (from telomere to centromere) are located on a 5547 bp genomic fragment on chromosome arm 3l at cytological position 66d8. the three genes are tightly linked, and flanked by two relatively large genes with unknown function. we have taken a comparative genomic approach to investigate the evolutionary history of the three genes. to this end we isolated a drosophila virilis 7.3 kb genomic fragment which is ho ... | 2002 | 11814692 |
| brachyury proteins regulate target genes through modular binding sites in a cooperative fashion. | brachyury proteins, a conserved subgroup of the t domain transcription factors, specify gut and posterior mesoderm derivatives throughout the animal kingdom. the t domain confers dna-binding properties to brachyury proteins, but little is known how these proteins regulate their target genes. we characterized a direct target gene of the drosophila brachyury-homolog brachyenteron. brachyenteron activates the homeobox gene orthopedia in a dose-dependent manner via multiple binding sites with the co ... | 2002 | 11850413 |
| xena, a full-length basal retroelement from tetraodontid fish. | mobile genetic elements are ubiquitous throughout the eukaryote superkingdom. we have sequenced a highly unusual full-length retroelement from the fugu fish, takifugu rubripes. this element, which we have named xena, is similar in structure and sequence to the penelope retroelement from drosophila virilis and consists of a single long open reading frame containing a reverse transcriptase domain flanked by identical direct long terminal repeat (ltr) sequences. these ltrs show an organization simi ... | 2002 | 11861884 |
| the cloning and expression of the gene encoding organ-specific esterase s from the genome of drosophila virilis. | we have cloned the gene for the esterase s isozymes complex from the genome of drosophila virilis in pbr322. esterase s is an enzyme which is specifically synthesized in the ejaculatory bulbs of d. virilis adult males. the gene for the esterase s isozyme complex (ests) has been localized in band 2g5e of chromosome ii. poly(a)+ rna prepared from ejaculatory bulbs actively hybridizes with this band. a cloned 15-kb fragment of d. virilis dna (pve9) also hybridizes with band 2g5e. the area encoding ... | 1983 | 11894894 |
| two divergent species of the virilis group, drosophila littoralis and drosophila virilis, share a replacement polymorphism at the fused locus. | 2002 | 11919301 | |
| in situ hybridisation analysis of the x-linked genes in the species of the virilis group of drosophila. | when estimating the level of dna sequence variation within and between populations or when planning qtl analysis, it is essential to know the location of the genes under study. in the present work, five x chromosomal genes, earlier localised in drosophila virilis and d. littoralis, were mapped by in situ hybridisation on the larval polytene chromosomes of four other virilis group species, d. a. americana, d. flavomontana, d. lacicola and d. montana. conjugation of x chromosomes of the most inter ... | 2002 | 11990762 |
| lift and power requirements of hovering flight in drosophila virilis. | the lift and power requirements for hovering flight in drosophila virilis were studied using the method of computational fluid dynamics. the navier-stokes equations were solved numerically. the solution provided the flow velocity and pressure fields, from which the unsteady aerodynamic forces and moments were obtained. the inertial torques due to the acceleration of the wing mass were computed analytically. on the basis of the aerodynamic forces and moments and the inertial torques, the lift and ... | 2002 | 12124366 |
| organization, sequence, and phylogenetic analysis of the ribosomal protein s3 gene from drosophila virilis. | ribosomal protein s3 (rps3) is a multifunctional ribosomal protein: it is a structural and functional component of the ribosome, and also a dna repair enzyme involved in the dna base excision repair pathway. here we cloned and characterized the genomic organization of the ribosomal protein s3 gene (rps3) homolog in drosophila virilis. we then compared gene structure and protein sequences of rps3 from vertebrates, invertebrates, and plants. these comparisons revealed that rps3 genes from plants t ... | 2002 | 12234676 |
| nucleotide and repeat length variation at the nona gene of the drosophila virilis group species and its effects on male courtship song. | genes found to affect male courtship song characters in drosophila melanogaster are good candidates when tracing genes responsible for species-specific songs in other drosophila species. it has previously been shown that thr-gly repeat length variation at the period gene affects song traits in d. melanogaster, which gives the repetitive regions a special interest. in this work, we have characterised the patterns of nucleotide variation for gene regions containing two gly and one gln-ala repeat i ... | 2002 | 12403170 |
| penelope retroelements from drosophila virilis are active after transformation of drosophila melanogaster. | the penelope family of retroelements was first described in species of the drosophila virilis group. intact elements encode a reverse transcriptase and an endonuclease of the uvrc type, which may play a role in penelope integration. penelope is a key element in the induction of d. virilis hybrid dysgenesis, which involves the mobilization of several unrelated families of transposable elements. we here report the successful introduction of penelope into the germ line of drosophila melanogaster by ... | 2002 | 12451171 |
| mussel map, a major gonad-duct esterase-like protein, is released into sea water as a dual constituent of the seminal fluid and the spermatozoon. | our interest in the comparative analysis of male reproductive-tract esterases in different animal groups has led us to undertake a detailed study of the mytilus galloprovincialis male-associated polypeptide (map) throughout the mussel gonad-duct tract and at spawning. the results of this work indicate that map is a major protein in m. galloprovincialis semen, with dual presence in both sperm cells and cell-free seminal fluid. shortly after spawning, the released sperm mass is subdivided in diffu ... | 2003 | 12477901 |
| developmental regulation of alternative exon usage in the house fly vssc1 sodium channel gene. | sequence analysis of cdna clones amplified by pcr from house fly ( musca domestica l.) vssc1 voltage-sensitive sodium channel alpha subunit transcript templates identified 11 putative alternatively spliced exons. nine of these corresponded to the 7 optional exons (designated a, b, e, f, h, i, and j) and 2 mutually exclusive exons (designated c/d) identified previously in the orthologous para sodium channel alpha subunit genes of drosophila melanogaster and drosophila virilis, whereas two segment ... | 2002 | 12488972 |
| retroelements containing introns in diverse invertebrate taxa. | we report that two structurally similar transposable elements containing reverse transcriptase (rt), penelope in drosophila virilis and athena in bdelloid rotifers, have proliferated as copies containing introns. the ability of penelope-like elements (ples) to retain introns, their separate phylogenetic placement and their peculiar structural features make them a novel class of eukaryotic retroelements. | 2003 | 12524543 |
| complex inheritance of male courtship song characters in drosophila virilis. | we analyzed the inheritance of two male courtship song characters, number of pulses in a pulse train, and length of a pulse train in drosophila virilis. biometric analyses of song differences among 16 crosses over three generations (parental and reciprocal f1, f2, and backcrosses) were performed using two different approaches. the joint scaling test revealed significant additive and dominance components, and also significant additive interaction between maternal and progeny genotypes. the direct ... | 2003 | 12645818 |
| role of x chromosomal song genes in the evolution of species-specific courtship songs in drosophila virilis group species. | in the drosophila virilis group the males of the virilis phylad species produce courtship song consisting of pulse trains with no pauses between successive sound pulses, whereas the males of the montana phylad species produce songs with clear pauses between the sound pulses. we obtained song data for f1 hybrids between d. virilis (representing the virilis phylad) or d. flavomontana (representing the montana phylad) females and the males of several species of the d. virilis group to study the int ... | 2003 | 12645819 |
| [the unusual mobile element penelope and its behavior in distant drosophila species]. | the retroelement penelope isolated from drosophila virilis has a very unusual structure and codes for reverse transcriptase and an endonuclease belonging to the uvrc type. as shown previously, penelope is a key element in induction of the hybrid dysgenesis syndrome described in d. virilis, which also involves mobilization of several unrelated mobile element families. here we report a successful introduction of penelope into the d. melanogaster genome by p element-mediated transformation. in the ... | 2003 | 12669424 |
| the prat purine synthesis gene duplication in drosophila melanogaster and drosophila virilis is associated with a retrotransposition event and diversification of expression patterns. | the drosophila melanogaster prat gene encodes amidophosphoribosyltransferase (prat; ec 2.4.2.14), which performs the first step in de novo purine nucleotide synthesis. prat mutations have a recessive lethal phenotype that is found for other genes encoding enzymes in this pathway. the d. melanogaster genome project has revealed a second gene, cg10078 or prat2, encoding a protein with 76% amino acid sequence identity with prat. the two genes map to different arms of chromosome 3 and have different ... | 2003 | 12698299 |
| [ulysses retrotransposon aspartate proteinase (drosophila virilis)]. | retrotransposones are mobile genetic elements occurring in genomes of bacteria, plants or animals. retrotransposones were found to contain nucleotide sequences encoding proteins which are homological to retroviral aspartic proteinases. our research has been focused on ulysses which is mobile genetic element found in drosophila virilis. we suggested a primary structure of ulysses proteinase using comparative analysis of amino acid sequences of retroviral proteinases and proteinases from retrotran ... | 2002 | 12698554 |
| functional conservation of the sex-lethal sex determining promoter, sxl-pe, in drosophila virilis. | the primary sex determination signal in drosophila melanogaster, the ratio of x chromosomes to autosomes, sets the activity state of the switch gene, sex-lethal ( sxl), by regulating the establishment promoter, m-sxl-pe. we have identified and characterized the establishment promoter, v-sxl-pe, of the distantly related species drosophila virilis. like melanogaster, the virilis sxl-pe is organized into four sub-domains: the sxl-pe mrna leader and exon e1 of sxl protein, the core promoter, the sex ... | 2003 | 12739140 |
| transcription control of a gene for drosophila transcription factor, dref by dre and cis-elements conserved between drosophila melanogaster and virilis. | a dna replication-related element (dre)-binding factor (dref) has been revealed to be an important transcription factor for activating promoters of cell proliferation and differentiation related genes. the amino acid sequences of dref are conserved in evolutionary separate drosophila species, drosophila melanogaster (dm) and drosophila virilis (dv) in three regions. in the present study, evidence was obtained that there are several highly conserved regions in the 5' flanking region between the d ... | 2003 | 12758126 |
| turnover of binding sites for transcription factors involved in early drosophila development. | despite the importance of cis-regulatory regions in evolution, little is know about their evolutionary dynamics. in this report, we analyze the process of evolution of binding sites for transcription factors using as a model a well characterized system, the drosophila early developmental enhancers. we compare the sequences of eight enhancer regions for early developmental genes between drosophila melanogaster and other two species, drosophila virilis and drosophila pseudoobscura, searching for t ... | 2003 | 12801649 |
| molecular cloning, sequencing and alternative splicing of the acid phosphatase (acph) gene from drosophila virilis. | the acph gene of drosophila virilis was isolated from a genomic library and sequenced. the gene was organized into 6 exons interrupted by 5 introns. 5' and 3' race identified 3 transcripts ascribed to alternative splicing. one of them coded for 435 amino acid residues; and the others, for 403 amino acids. the shorter ones lacked 34 residues at the c-terminus end encoded by exon 6, which has been implicated in anchoring the protein to the lysosomal membrane. | 2003 | 12825355 |
| evolution of gab family adaptor proteins. | the gab/dos/soc-1 proteins form a family of multi-adaptor/scaffolding proteins involved in receptor tyrosine kinase signaling. to further understanding of the gab family and the drosophila dos protein in particular, we isolated a dos homolog from both drosophila pseudoobscura and drosophila virilis and compared their gene structures and protein sequences with the rest of the gab family. the presence of two conserved introns confirmed that the dos and gab genes are orthologous, but the caenorhabd ... | 2003 | 12853137 |
| heat stress affects oogenesis differently in wild-type drosophila virilis and a mutant with altered juvenile hormone and 20-hydroxyecdysone levels. | the link between reproduction and environmental signals is poorly understood at the physiological, genetic and molecular levels. we describe a mutant strain of drosophila virilis that has altered responses to heat stress. heat stress in wild-type females results in oocyte maturation delays, degradation of early vitellogenic egg chambers, inhibition of yolk protein gene expression in follicle cells and accumulation of mature oocytes. the mutant females have increased levels of ecdysteroids and de ... | 2003 | 12864919 |
| [mitochondrial dna polymorphism in the natural populations of the drosophila virilis species group]. | restriction fragment length polymorphism (rflp) analysis has been used to evaluate mitochondrial dna (mtdna) variation in 12 sibling species forming the drosophila virilis species group. the variation thresholds corresponding to the interspecific and interstrain levels have been determined. the results indicate that interspecific hybridization has significantly contributed to the evolutionary history of the virilis species group. | 2003 | 12884514 |
| the drosophila melanogaster btb proteins bric à brac bind dna through a composite dna binding domain containing a pipsqueak and an at-hook motif. | the bric à brac (bab) locus is composed of two paralogous genes, bab1 and bab2, in drosophila melanogaster. bab1 and bab2 are nuclear proteins that contain a broad complex, tramtrack, bric à brac/poxviruses and zinc-finger (btb/poz) domain. many btb/poz proteins are transcriptional regulators of which the majority contain c(2)h(2) zinc-finger motifs. there is no detectable zinc-finger motif in either bab protein. however, they share the bab conserved domain (babcd) that is highly conserved betwe ... | 2003 | 12954775 |
| temporal comparison of broad-complex expression during eggshell-appendage patterning and morphogenesis in two drosophila species with different eggshell-appendage numbers. | a central question in biology is how developmental mechanisms are altered to bring about morphological evolution. drosophilids boast a remarkable diversity in eggshell-appendage number-from as few as one to as many as nine, depending on the species. appendage patterning in drosophila melanogaster is well characterized, inviting candidate-gene-based approaches that identify the developmental mechanisms underlying drosophilid eggshell diversity. previous studies show that a combination of epiderma ... | 2003 | 12971997 |
| cytochrome c oxidase activity during the metamorphosis of drosophila virilis. | 1952 | 12984106 | |
| some aspects of the melanotic tumor occurring in drosophila virilis. | 1954 | 13183262 | |
| the effects of nitric oxide on radiation damage in drosophila virilis and drosophila melanogaster. | 1961 | 13690620 | |
| biological damage in the mature sperm of drosophila virilis in oxygen and nitrogen with different dose intensities of gamma rays. | 1962 | 13860350 | |
| modification of v-type position effects in drosophila virilis. | 1962 | 13908922 | |
| the induction of dominant lethal mutations in x-irradiated drosophila virilis oocytes. | 1963 | 14027706 | |
| some characteristics of the salivary gland secretion of drosophila virilis. | 1963 | 14080668 | |
| some effects of nitric oxide and oxygen on dominant lethal production in x-irradiated drosophila virilis males. | 1963 | 14102807 | |
| the lamellar systems of cytoplasmic membranes in dividing spermatogenic cells by drosophila virilis. | spermatogenic cells of drosophila virilis were studied by light and electron microscopy. the persistence of a "nuclear wall" during the meiotic divisions has been reported by a number of early cytologists, but this interpretation has been a subject of debate. electron micrographs of dividing spermatocytes reveal the presence of multiple layers of paired membranes surrounding the nuclear region. these lamellar membrane systems are not typical of the nuclear envelope, but were interpreted as such ... | 1960 | 14406005 |
| het-a elements in drosophila virilis: retrotransposon telomeres are conserved across the drosophila genus. | drosophila melanogaster telomeres are composed of two retrotransposons, het-a and tart. drosophila virilis has recently been shown to have telomere-specific tart elements with many of the characteristics of their d. melanogaster homologues. we now report identification of the second telomere-specific retrotransposon, het-a, from d. virilis. these results show that het-a and tart have been maintaining telomeres in drosophila for more than the 60 million years that separate d. melanogaster and d. ... | 2003 | 14614149 |
| qtl analysis of variation in male courtship song characters in drosophila virilis. | we have used a quantitative trait locus (qtl) mapping approach to study the genetic basis of differences between two drosophila virilis strains representing extreme phenotypes in two song characters, the number of pulses in a pulse train (pn) and the length of a pulse train (ptl). variation in these characters among 520 f2 males was studied by single-marker analysis and composite interval mapping (cim) using a recombination linkage map constructed for 26 microsatellite markers. in single-marker ... | 2004 | 14679394 |
| [morphometric analysis of male genitalia in sibling species of drosophila virilis sturt]. | the structure of male genital organs in sibling species of the virilis group of drosophila was examined using methods of multivariate statistics. the differences among these species were estimated using 33 indices and 2 angle parameters. the intraspecific and interspecific correlation structure of the examined characters and the order of their divergence were established. the key characters with respect to forming interspecific differences in the virilis species group were identified. based on t ... | 2004 | 15065425 |
| the role of juvenile hormone in the control of reproductive function in drosophila virilis under nutritional stress. | to investigate the role of juvenile hormone (jh) in the control of drosophila reproduction under stress, jh degradation and reproduction were studied under nutritional stress and jh treatment in drosophila virilis females of wild type (wt) and a heat stress (hs) mutant: this mutant does not respond to heat stress by alterations in jh metabolism and has decreased jh level and fertility under normal conditions. one day of starvation results in a decrease of jh degradation, a delay in oocyte matura ... | 2004 | 15081825 |
| evolution of the trans-splicing drosophila locus mod(mdg4) in several species of diptera and lepidoptera. | the modifier of mdg4 (mod[mdg4]) locus of drosophila melanogaster (dme) encodes chromatin proteins which are involved in position effect variegation, establishment of chromatin boundaries, nerve pathfinding, meiotic chromosome pairing and apoptosis. it was recently shown that mrna trans-splicing is involved in the generation of at least 26 different mod(mdg4) transcripts. here, we show that a similar complex mod(mdg4) locus exists in drosophila pseudoobscura (dps), drosophila virilis (dvi), anop ... | 2004 | 15094203 |
| new aspartic proteinase of ulysses retrotransposon from drosophila virilis. | this work is focused on the investigation of a proteinase of ulysses mobile genetic element from drosophila virilis. the primary structure of this proteinase is suggested based on comparative analysis of amino acid sequences of aspartic proteinases from retroviruses and retrotransposons. the corresponding cdna fragment has been cloned and expressed in e. coli. the protein accumulated in inclusion bodies. the recombinant protein (12 kd) was subjected to refolding and purified by affinity chromato ... | 2004 | 15236611 |
| a multilocus microsatellite phylogeny of the drosophila virilis group. | we used a set of 48 polymorphic microsatellites derived from drosophila virilis to infer phylogenetic relationships in the d. virilis clade. consistent with previous studies, d. virilis and d. lummei were the most basal species of the group. within the d. montana phylad, the phylogenetic relationship could not be resolved. special attention was given to the differentiation between d. americana texana, d. americana americana and d. novamexicana. significant differences between these three groups ... | 2004 | 15241464 |
| a kunitz type protease inhibitor related protein is synthesized in drosophila prepupal salivary glands and released into the moulting fluid during pupation. | from the drosophila virilis late puff region 31c, we microcloned two neighbouring genes, kil-1 and kil-2, that encode putative kunitz serine protease inhibitor like proteins. the kil-1 gene is expressed exclusively in prepupal salivary glands. using a size mutant of the kil-1 protein and maldi-tof analysis, we demonstrate that during pupation this protein is released from the prepupal salivary glands into the pupation fluid covering the surface of the pupa. 3-d-structure predictions are consiste ... | 2004 | 15262289 |
| new unusual miniature-like wing mutation in drosophila virilis. | a new recessive, sex-linked, nonlethal in the homozygote, wing mutation in drosophila virilis was studied using a hybridological assay, light microscopy, and transmission electron microscopy. the mutants have abnormally small wings; the phenotype is attributed to a cell-autonomous reduction in the size of the epidermal cells of the differentiating wing. the phenotype is also characterized by abnormally oriented wing hairs, wavy wing edge, temperature sensitivity, and some abnormalities in the wi ... | 2004 | 15281056 |
| [helical structure revealed upon the beta heterochromatin of polytene chromosome of drosophila virilis]. | by using conventional electron microscopy, beta heterochromatin of polytene chromosome of drosophila virilis was composed of 80-100nm chromonemas. helical structures consisting of the 30nm chromatin fibres were identified in the chromonema. based on these observations, the radial loop model and the multiple coiling model were discussed. | 2002 | 15344321 |
| reverse transcriptase and endonuclease activities encoded by penelope-like retroelements. | penelope-like elements are a class of retroelement that have now been identified in >50 species belonging to at least 10 animal phyla. the penelope element isolated from drosophila virilis is the only transpositionally active representative of this class isolated so far. the single orf of penelope and its relatives contains regions homologous to a reverse transcriptase of atypical structure and to the giy-yig, or uri, an endonuclease (en) domain not previously found in retroelements. we have exp ... | 2004 | 15465912 |
| evolution and arrangement of the hsp70 gene cluster in two closely related species of the virilis group of drosophila. | to investigate the genetic basis of differing thermotolerance in the closely related species drosophila virilis and drosophila lummei, which replace one another along a latitudinal cline, we characterized the hsp70 gene cluster in multiple strains of both species. in both species, all hsp70 copies cluster in a single chromosomal locus, 29c1, and each cluster includes two hsp70 genes arranged as an inverted pair, the ancestral condition. the total number of hsp70 copies is maximally seven in the ... | 2004 | 15480729 |
| trans-splicing of the mod(mdg4) complex locus is conserved between the distantly related species drosophila melanogaster and d. virilis. | the modifier of mdg4, mod(mdg4), locus in drosophila melanogaster represents a new type of complex gene in which functional diversity is resolved by mrna trans-splicing. a protein family of >30 transcriptional regulators, which are supposed to be involved in higher-order chromatin structure, is encoded by both dna strands of this locus. mutations in mod(mdg4) have been identified independently in a number of genetic screens involving position-effect variegation, modulation of chromatin insulator ... | 2005 | 15520256 |
| a genealogical view of chromosomal evolution and species delimitation in the drosophila virilis species subgroup. | chromosomal arrangement was a historically important character used for defining taxonomic boundaries. the drosophila virilis species group exhibits a series of chromosomal rearrangements, and the resulting differences among karyotypes were primary characters originally used to define taxa within the group. however, some chromosomally divergent forms have not been sufficiently resolved in phylogenetic reconstructions of dna sequences from several nuclear genes. sequences of mitochondrial regions ... | 2004 | 15522794 |
| [novel miniature-like m42 mutant of drosophila virilis. unusual microstructure of wing fibres]. | distinctive defects of cuticular wing vein microstructure in drosophila virilis miniature-like m42 allele and the possible role of m42 locus in cytoskeleton reorganization during of wing morphogenesis are discussed. | 2015 | 15619989 |
| [juvenile hormone controls egg laying and fertility in drosophila virilis during starvation]. | degradation of juvenile hormone and reproductive function during starvation and experimental increase of the juvenile hormone titer were studied in wild type and mutant d. virilis females incapable to respond to heat stress by changes in juvenile hormone metabolism and fertility. after 24-hour starvation, the females of both lines were characterized by a decreased level of juvenile hormone degradation, 24-hour delay of egg laying, increased egg laying within 3 h after the termination of starvati ... | 2006 | 15624776 |
| the retrotransposon tv1 forms infectious virus-like particles in some lines of drosophila virilis. | 2005 | 15846990 | |
| juvenile hormone, 20-hydroxyecdysone and dopamine interaction in drosophila virilis reproduction under normal and nutritional stress conditions. | to elucidate the role of the juvenile hormone (jh) in the control of drosophila reproduction under stress, jh degradation, dopamine (da) content and reproduction were studied upon 20e treatment in drosophila virilis females of wild type (wt) and a mutant, with increased 20e level and decreased fertility, under normal and nutritional stress conditions. 20e treatment of wt flies for 7 days results in an increase of da content in young females, but a decrease in mature females, a decrease of jh deg ... | 2005 | 15890185 |
| effects of dopamine on juvenile hormone metabolism and fitness in drosophila virilis. | the effects of dopamine (da) on juvenile hormone (jh) metabolism and fitness (estimated as fecundity and viability levels under heat stress (38 degrees c)) in drosophila virilis have been studied. an increase of da level obtained by feeding with da reduced fitness of wild-type (wt) flies under stress, and decreased jh degradation in young wt females while increasing it in sexually mature wt females. a decrease in da levels resulted from 3-iodo-tyrosine treatment and caused a decrease in jh degra ... | 2005 | 15967456 |
| experimental decrease in dopamine level dramatically decreases drosophila virilis fitness. | 2016 | 16003876 | |
| penelope-like elements--a new class of retroelements: distribution, function and possible evolutionary significance. | here we describe a new class of retroelements termed ple (penelope-like elements). the only transpositionally active representative of this lineage found so far has been isolated from drosophila virilis. this element, penelope, is responsible for the hybrid dysgenesis syndrome in this species, characterized by simultaneous mobilization of several unrelated te families in the progeny of dysgenic crosses. several lines of evidence favor the hypothesis of recent penelope invasion into d. virilis. m ... | 2005 | 16093704 |
| [variation of 3'-terminal fragment of 16s rrna gene in closely related species of drosophila virilis group]. | primary sequence of 3-terminal fragment of the mitochondrial 16s rrna gene has been determined in 12 drosophila species of the virilis group. the functionally important elements in secondary structure of the rna product were defined. the region corresponding to the peptidyltransferase center has been localized. variation of the 3'-terminal region of 16s rrna gene has been described in 12 species of the virilis group. phylogeny of the drosophila virilis species group is discussed. | 2005 | 16161625 |
| [problems of studying the genetic background of speciation as examplified by drosophila virilis group]. | possible ways of studying the genetic bases of speciation have been shown on the example of published data and authors' results. the data were used, which were obtained on different drosophila species. possible application of mapping by localization of the gene of quantitative traits and genetic transformation for solution of the problems of speciation is discussed. | 2015 | 16245566 |
| evidence for maternally transmitted small interfering rna in the repression of transposition in drosophila virilis. | hybrid dysgenesis in drosophila is a syndrome of gonadal atrophy, sterility, and male recombination, and it occurs in the progeny of crosses between males that harbor certain transposable elements (tes) and females that lack them. known examples of hybrid dysgenesis in drosophila melanogaster result from mobilization of individual families of tes, such as the p element, the i element, or hobo. an example of hybrid dysgenesis in drosophila virilis is unique in that multiple, unrelated families of ... | 2005 | 16247000 |
| independence of genetic geographical variation between photoperiodic diapause, circadian eclosion rhythm, and thr-gly repeat region of the period gene in drosophila littoralis. | drosophila littoralis is a latitudinally widespread european species of the drosophila virilis group. the species has ample genetic variation in photoperiodism (adult diapause) and circadian rhythmicity (pupal eclosion rhythm), with adaptive latitudinal clines in both of them. the possible common genetic basis between the variability of photoperiodism and circadian rhythms was studied by a long-term crossing experiment. a northern strain (65 degrees n) having long critical day length (cdl = 19.9 ... | 2006 | 16461980 |
| conserved domains control heterochromatin localization and silencing properties of su(var)3-7. | the drosophila protein su(var)3-7 is essential for fly viability, chromosome structure, and heterochromatin formation. we report that searches in silico and in vitro for homologues of su(var)3-7 were successful within, but not outside, the drosophila genus. protein sequence homology between the distant sibling species drosophila melanogaster and drosophila virilis is low, except for the general organization of the protein and three conserved motives: seven widely spaced zinc fingers in the n-ter ... | 2006 | 16463146 |