Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| ovitrap surveys of dengue vector mosquitoes in chiang mai, northern thailand: seasonal shifts in relative abundance of aedes albopictus and ae. aegypti. | aedes aegypti (l.) and aedes albopictus (skuse) were surveyed using ovitraps in residential areas in chiang mai, northern thailand. egg populations (both species inclusive) remained low in the dry season, but increased/decreased exponentially during the first/latter half of the rainy season, respectively. this seasonal pattern was similar to the seasonal distribution of dengue haemorrhagic fever cases in the area. during the dry season (november-march) ae.aegypti was dominant in urban and indoor ... | 1988 | 2980189 |
| morphology of bluetongue virus-infected aedes albopictus (c6/36) cell culture. | morphological changes observed in bluetongue virus (btv)-infected aedes albopictus (c6/36) suspension cell culture at 28 c were compared to those observed in mammalian stationary cell culture at 37 c. the presence of cytoplasmic macrotubules, viroplasms and progeny virions was confirmed and appears to be the same as seen in mammalian cell culture. in addition progeny virions were observed budding from cell membranes where they appeared to acquire a lipoprotein envelope. intranuclear macrotubules ... | 1985 | 2989866 |
| recent vesicular stomatitis virus infection detected by immunoglobulin m antibody capture enzyme-linked immunosorbent assay. | we developed an enzyme-linked immunosorbent assay (elisa) that was capable of detecting immunoglobulin m (igm) antibody to vesicular stomatitis virus (vsv) in the sera of experimentally and naturally infected cattle and horses. the detection of igm in the sera of these animals permitted an estimate of the recency of infection by vsv serotype new jersey. a vsv serotype new jersey epizootic strain isolated from a horse and passed once in an aedes albopictus cell line was used to infect a horse and ... | 1985 | 3001132 |
| site of suppression of banzi viral replication by an antiviral factor released from aedes albopictus cells persistently infected with banzi virus. | the ability of the antiviral factor present in culture medium of aedes albopictus cells persistently infected with the flavivirus, banzi virus, to inhibit the replication of banzi virus was examined. the anti-banzi viral factor did not inhibit the uncoating of the virion. levels of viral rna were markedly reduced in mosquito cells treated with the antiviral factor. syntheses of negative-strand and of positive-strand viral rna species were inhibited to approximately the same extent. this inhibiti ... | 1985 | 3002069 |
| study of receptors for vesicular stomatitis virus in vertebrate and invertebrate cells. | early interactions between vesicular stomatitis virus (vsv) and susceptible cells were examined in cell lines of mammalian (hela), bird (cer), piscine (epc) and arthropod (aedes albopictus) origin showing different permissiveness to vsv growth. the chemical nature of receptors was investigated either by modification of cell surfaces with different enzymes or by competition for vsv binding between extracted membrane components and whole cells. results obtained indicate that in all cell models, me ... | 1986 | 3018449 |
| susceptibility of indonesia colonies of aedes aegypti and aedes albopictus mosquitoes to experimental infection with chikungunya virus. | 1986 | 3023740 | |
| bacillus thuringiensis var. israelensis delta-endotoxin. cloning and expression of the toxin in sporogenic and asporogenic strains of bacillus subtilis. | a plasmid-borne gene from bacillus thuringiensis var. israelensis encoding a 27,340 mr insecticidal delta-endotoxin has been cloned on a bifunctional multicopy plasmid in a wild-type sporogenic strain and two asporogenic mutants of bacillus subtilis. the delta-endotoxin gene is expressed at a low level during vegetative growth in all three strains, but the synthesis of the toxin increases markedly during the third hour of stationary phase for both the sporogenic strain and an asporogenic mutant ... | 1986 | 3025453 |
| entry pathway of vesicular stomatitis virus into different host cells. | a biochemical and morphological investigation of the mechanism of entry of vesicular stomatitis virus (vsv) into host cells of mammalian (hela), avian (cer), piscine (epc) and arthropod (aedes albopictus) origin, is described. vsv was capable of infecting all cell lines tested by a endosome- and/or a lysosome-dependent step since ammonium chloride and amantadine blocked the early stages of infection. complement-dependent immune lysis of infected host cells provided evidence that in none of the f ... | 1987 | 3029282 |
| ultrastructural studies of kunjin virus-infected aedes albopictus cells. | ultrastructural changes in aedes albopictus cells infected with kunjin virus were characterized from 12 to 72 h post-infection. early in infection (16h), there were no prominent ultrastructural changes except for an increase in the number of vacuoles in the cytoplasm. as the infection progressed the rough endoplasmic reticulum appeared to lengthen and whorls of fibres were observed within some vacuoles. virus particles were observed in small numbers scattered in the cytoplasm between 24 to 30 h ... | 1987 | 3029292 |
| sindbis virus mutants resistant to mycophenolic acid and ribavirin. | previous work from this laboratory has demonstrated a correlation between the inhibition by ribavirin (rbv), mycophenolic acid (mpa), or 2-amino thiadiazole (tda) of sindbis virus replication in aedes albopictus mosquito cells and a reduction in cellular gtp levels. this reduction in gtp results from the inhibition by these drugs of inosine monophosphate dehydrogenase (impdh), the first enzyme specific for the de novo synthesis of gmp. by serial passage of sv in a. albopictus cells in the presen ... | 1987 | 3033882 |
| aedes albopictus in the united states: rapid spread of a potential disease vector. | aedes albopictus, the asian "tiger mosquito," was found in houston, texas, in 1985. aedes albopictus is primarily a forest edge inhabiting species that has readily adapted to the container habitats produced by humans. although not yet incriminated in the spread of any disease in the americas, it has been repeatedly implicated in epidemic dengue and dengue hemorrhagic fever transmission in asia. it is a competent laboratory vector of la crosse, yellow fever and other viruses, and can transovarial ... | 1988 | 3058869 |
| [pathogenicity of spiroplasma sabaudiense (mollicute) for the cells (c6/36) of aedes albopictus (insecta: diptera) in vitro]. | an initial stock of spiroplasma sabaudiense had been maintained in cell-free medium for 2-3 years. subsequent passages in leibovitz's medium l-15 in the presence of aedes albopictus (c6/36) cells dramatically increased the pathogenicity of s. sabaudiense towards these cells. cytopathogenicity included production of syncytia, an increase in the number of mitochondria and alteration of their morphology, vacuolisation and a reduction in the rate of growth. cell lysis was associated with cytadsorpti ... | 1988 | 3064943 |
| the biology of aedes albopictus. | the biology of aedes albopictus is reviewed, with emphasis on studies of ecology and behavior. the following topics are discussed: distribution and taxonomy, genetics, medical importance, habitat, egg biology, larval biology, adult biology, competitive interactions, comparative studies with aedes aegypti, population dynamics, photoperiodism, and surveillance and control. | 1988 | 3068349 |
| aedes albopictus introduction--texas. | 1986 | 3080671 | |
| transovarial maintenance of san angelo virus in sequential generations of aedes albopictus. | an aedes albopictus line originally selected for efficient transovarial transmission (tot) of san angelo (sa) virus displayed a progressive decline in filial infection rate (fir) whenever artificial selection pressure was relaxed. this observation brought into question whether the efficiency of tot in this vector-virus model was sufficiently high to permit persistent vertical transmission over numerous freely reproducing generations. by tracing descendants of individual transovarially infected f ... | 1986 | 3082235 |
| aedes albopictus infestation--united states, brazil. | 1986 | 3090418 | |
| update: aedes albopictus infestation--united states. | 1986 | 3093836 | |
| the surface charge of l-a9 cells and aedes albopictus cells infected with marituba (bunyaviridae) virus. | the surface charge of marituba virus infected l-a9 cells and aedes albopictus cells was estimated by direct measurement of their electrophoretic mobilities. uninfected l-a9 cells and a. albopictus cells have mean electrophoretic mobilities of -1.083 microns/s x cm/v and -1.019 microns/s x cm/v, respectively. in marituba virus infected l-a9 cells a progressive decline in the electrophoretic mobility was observed. in contrast, in marituba virus infected a. albopictus cells the electrophoretic mobi ... | 1987 | 3111119 |
| update: aedes albopictus infestation--united states. | 1987 | 3119976 | |
| an orbivirus of mosquitoes which induces co2 sensitivity in mosquitoes and is lethal for rabbits. | an orbivirus, jkt-7400, isolated from culex mosquitoes in indonesia, replicated to a high titer and induced cytopathic effects in aedes albopictus cell cultures. the virus produced lethal sensitivity to carbon dioxide in culex and aedes mosquitoes as well as in drosophila melanogaster fruit flies but was not the agent of the hereditary sensitivity to carbon dioxide previously described for culex quinquefasciatus. when injected intravenously in high doses, jkt-7400 virus was lethal for rabbits, a ... | 1988 | 3136255 |
| effect of continuous heat stress on cell growth and protein synthesis in aedes albopictus. | aedes albopictus (clone c6/36) cells, which normally grow at 28 degrees c, were maintained at a supraoptimal temperature of 37 degrees c. the effect of continuous heat stress (37 degrees c) on cell growth was analyzed as were the modifications occurring with protein synthesis during short- and long-term heat stress. we observed that cells in lag or exponential growth phase, present inhibition of cell growth, and cells in the lag phase showed more sensitivity to death than cells growing exponenti ... | 1988 | 3192626 |
| vector competence of a houston, texas strain of aedes albopictus for rift valley fever virus. | 1988 | 3193106 | |
| a survey of mosquitoes breeding in used tire stockpiles in connecticut. | a larval survey of nine used tire disposal sites in connecticut, was conducted from june through october 1987. no larvae of aedes albopictus were uncovered indicating that this mosquito is not yet established in the state. however, eight other mosquito species in four genera were found. the most abundant and widely distributed species found breeding in permanent tire dumps were ae. atropalpus, ae. triseriatus and culex restuans. culex pipiens was the second most common culex species followed by ... | 1988 | 3199116 |
| susceptibility status of aedes albopictus to three topically applied adulticides. | the baseline susceptibility of aedes albopictus (sabah strain) to malathion was determined. both laboratory-colonized and field-collected ae. albopictus at harris county, tx are resistant to malathion but susceptible to scourge. the acute toxicity of bendiocarb to ae. albopictus is confounded by its rapid knockdown and delayed recovery syndrome of poisoning. | 1988 | 3199119 |
| aedes albopictus in florida. | 1988 | 3199126 | |
| effects of dengue-1 infection in aedes albopictus on its susceptibility to malathion. | 1988 | 3199129 | |
| arboviruses recovered from sentinel cattle using several virus isolation methods. | a group of 20 sentinel steers was bled weekly for 5 months in 1986 and the blood samples were examined for arboviruses by inoculation firstly into embryonated chicken eggs (ece), baby mice, aedes albopictus cells and bhk21 monolayers. a second group of cattle was similarly examined for virus in 1987, except that baby mice were not used. viruses were recovered from 26% of the 878 weekly bleeds. the viruses identified consisted of 14 types belonging to the bluetongue, epizootic haemorrhagic diseas ... | 1988 | 3218074 |
| use of elevated temperatures to kill aedes albopictus and ae. aegypti. | 1988 | 3225579 | |
| breeding structure of a colonizing species: aedes albopictus (skuse) in peninsular malaysia and borneo. | the mosquito, aedes albopictus, has recently become established in a number of cities throughout the united states. an initial survey of allozyme and genotypic frequencies in u.s. populations (black et al., 1988) revealed an extensive amount of local differentiation of populations and suggested that much genetic drift may have accompanied colonization. a study of gene flow was initiated in native habitats of ae. albopictus in malaysia to determine if the result observed in the u.s. was a consequ ... | 1988 | 3230033 |
| [variations in the phenotypic expression of the western nile fever virus after reproduction in two aedes albopictus skuse, 1895 cell lines]. | 1988 | 3231175 | |
| aedes albopictus mosquitoes introduced into brazil: vector competence for yellow fever and dengue viruses. | 1988 | 3232189 | |
| [arbovirus infections on the island of nosy-be; serologic and entomologic findings]. | since 1977, the pasteur institute of madagascar has been studying, during six surveys, the arboviruses of nosy-be area, in the north-west of madagascar. 47.2 p. 100 out of 271 human sera and 11.3 p. 100 out of 150 animal sera (mostly from lemurs), tested for antibodies to 16 arboviruses by the haemagglutination inhibition test, are positive. the results show an important prevalence of flaviviruses. west-nile and dengue 1 viruses were probably circulating some years before the surveys. antibodies ... | 1988 | 3242421 |
| detection of dengue virus type 2 in aedes albopictus by nucleic acid hybridization with strand-specific rna probes. | a molecular hybridization technique with radiolabeled, strand-specific rna probes was developed to detect dengue virus type 2 rna in pools of infected aedes albopictus mosquitoes. one infected mosquito in a pool of 25 could be detected, corresponding to a dengue virus type 2 titer of 2.75 log10 50% tissue culture infectious doses. | 1988 | 3281980 |
| formation and maintenance of viroplasmic centers in tipula iridescent virus-infected mosquito cells with deranged cytoskeletons. | we have examined the role of cytoskeletal elements with respect to the formation and maintenance of viroplasmic centers (vcs) in tipula iridescent virus (tiv)-infected mosquito aedes albopictus (c6/36) cells. filamentous systems consisting of microtubules and microfilaments were detected by immunofluorescence microscopy. inoculation of cells with tiv resulted in an alteration of microtubule and microfilament organization whether or not vcs developed. the formation of short arrays of microtubules ... | 1987 | 3297704 |
| cytolytic activity and immunological similarity of the bacillus thuringiensis subsp. israelensis and bacillus thuringiensis subsp. morrisoni isolate pg-14 toxins. | the parasporal bodies of the mosquitocidal isolates of bacillus thuringiensis subsp. israelensis and b. thuringiensis subsp. morrisoni isolate pg-14 were compared with regard to their hemolytic and cytolytic activities and the immunological relatedness of the 28- and 65-kilodalton (kda) proteins that occur in both subspecies. the alkali-solubilized parasporal bodies of b. thuringiensis subsp. israelensis caused 50% lysis of human erythrocytes at 1.14 micrograms/ml, whereas those of b. thuringien ... | 1987 | 3300548 |
| dengue haemorrhagic fever in malaysia: a review. | the historical background, epidemiology and changing pattern of clinical disease as seen in malaysia is reviewed. the preliminary results of the longitudinal study of epidemiology of dengue in malaysia is also presented. studies led by rudnick et al. over some 18 years have established that the disease is endemically transmitted by both aedes aegypti and aedes albopictus causing illnesses ranging from mild febrile episodes through classical dengue fever, dengue haemorrhagic fever and the dengue ... | 1987 | 3324361 |
| characterization of the toxicity and cytopathic specificity of a cloned bacillus thuringiensis crystal protein using insect cell culture. | an insecticidal protein gene from bacillus thuringiensis var. aizawai was cloned in escherichia coli. the cloned gene expressed at a high level and the synthesized protein appeared as an insoluble, phase-bright inclusion in the cytoplasm. these inclusions were isolated by density gradient centrifugation, the isolated protein was activated in vitro by different proteolytic regimes and the toxicity of the resulting preparations was studied using insect cells grown in tissue culture. the inclusions ... | 1987 | 3330756 |
| aedes albopictus and arboviruses: a concise review of the literature. | aedes albopictus is an efficient vector of the four dengue viruses, and it is also capable of transovarially transmitting these viruses. aedes albopictus can also serve as a host and/or vector of several additional arboviruses, some of which are of considerable medical or veterinary importance. this review compiles previously published studies that have demonstrated an experimental or natural association between ae. albopictus and specific arboviruses. | 1986 | 3333791 |
| genetics of aedes albopictus. | aedes albopictus is an important vector of dengue fever and dengue hemorrhagic fever in southeast asia. its distribution extends from madagascar to hawaii and is currently expanding. from its proposed origin in southeast asia, ae. albopictus has migrated as far as mauritius and madagascar to the west and korea, japan, guam, hawaii and other pacific islands to the east. in the continental united states, it was originally reported in the texas area in august 1985 and is now well established in sev ... | 1986 | 3333792 |
| leads from the mmwr. update: aedes albopictus infestation--united states. | 1988 | 3336132 | |
| svlm21, a mutant of sindbis virus able to grow in aedes albopictus cells in the absence of methionine, shows increased sensitivity to s-adenosylhomocysteine hydrolase inhibitors such as neplanocin a. | inhibition of s-adenosylhomocysteine (adohcy) hydrolase by compounds such as neplanocin a (npa) leads to the build-up of adohcy and the inhibition of methyltransferase enzymes. whether assayed by efficiency of plaquing or virus yield, svlm21, a mutant of sindbis virus resistant to methionine deprivation, was more sensitive to npa than was the standard virus (svstd) from which it was derived. for example, whereas 10 micrograms npa/ml depressed the yield of svlm21 by more than 30-fold, the yield o ... | 1988 | 3347999 |
| exposure of semliki forest virus-infected baby hamster kidney cells to low ph leads to a proton influx and a rapid depletion of intracellular atp which in turn prevents cell-cell fusion. brief report. | the conditions needed to trigger semliki forest virus (sfv) induced cell-cell fusion from within (ffwi) of baby hamster kidney (bhk) cells differs from some other cells (e.g., aedes albopictus cells). in contrast to such cells it is mandatory for bhk cells to be brought back to a neutral ph after a short exposure to mildly acidic ph for fusion to occur. this phenomenon can be explained by a sudden drop of the intracellular ph observed after clamping the extracellular ph below 6.2--the ph require ... | 1988 | 3355372 |
| breeding structure of a colonising species: aedes albopictus (skuse) in the united states. | allelic and genotypic frequencies were monitored at seven enzymatic loci in newly established aedes albopictus (skuse) populations in the united states. populations were sampled within the cities of new orleans, louisiana, houston, texas, three counties surrounding houston, memphis, tennessee, jacksonville, florida, and evansville and indianapolis, indiana. unique alleles and relatively high levels of heterozygosity were detected in new orleans, houston, surrounding counties and indianapolis sug ... | 1988 | 3366625 |
| identification of cdnas corresponding to mosquito ribosomal protein genes. | sequences encoding mosquito (aedes albopictus) ribosomal proteins l8, l14 and l31 were identified from a cdna library made from size-selected polyadenylated mrna. candidate cdnas corresponding to moderately abundant mrnas were screened by translation of hybrid-selected transcripts in wheat-germ lysates. translation products were extracted with acetic acid and analyzed by electrophoresis in two dimensions in the presence of unlabeled ribosomal proteins. the identity of translation products that c ... | 2012 | 3382663 |
| effect of actinomycin d and cycloheximide on replication of sindbis virus in aedes albopictus (mosquito) cells. | production of sindbis virus in the presence of transcription and translation inhibitors was examined in three aedes albopictus cell lines. addition of cycloheximide to heat-resistant sindbis virus (svhr)-infected mosquito cells arrested viral rna synthesis completely, in contrast to the effects of this drug on virus-infected vertebrate cells. production of mature virus by both svhr (a variant commonly used as a wild-type virus) and sbamr (a mutant which is resistant to the effects of 18 h of pre ... | 1988 | 3392770 |
| survival of albumin, igg, igm, and complement (c3) in human blood after ingestion by aedes albopictus and phlebotomus papatasi. | the levels of albumin, igg, igm, and complement (c3) were determined by enzyme-linked immunosorbent assay in aedes albopictus and phlebotomus papatasi at regular intervals after feeding on human blood. albumin disappeared most rapidly; by 48 hr, detectable levels of albumin had decreased 100-fold. in contrast, igg and igm survived longer and were still detectable at low levels several days after ingestion. c3 was intermediate in its rate of degradation. in general, serum protein disappearance oc ... | 1988 | 3400799 |
| [a study on the characteristics of controlled-release tube and effectiveness of the slow release of phoxim on killing larvae of aedes albopictus]. | 1987 | 3449221 | |
| lactate dehydrogenase from cultured aedes albopictus cells: kinetic and isozyme analysis. | l(+) lactate dehydrogenase (ldh) activity from cultured cells of aedes albopictus was studied as a kinetic model of carbohydrate metabolism. enzyme kinetics were studied in the forward (lactate as substrate) and reverse (pyruvate as substrate) reactions and the apparent km values were obtained showing ldh higher affinity for pyruvate. the hill coefficient values for each substrate were similar and indicate the existence of only one binding site on the enzyme. isozyme analysis on cellulose-acetat ... | 1987 | 3454571 |
| factors affecting polybrene-mediated transfection of cultured aedes albopictus (mosquito) cells. | the polycation 1,5-dimethyl-1,5-diazaundecamethylene polymethobromide (polybrene) is superior to calcium phosphate for the introduction of purified dna into cultured aedes albopictus (mosquito) cells. adsorption of the polybrene-dna complex to mosquito cells was essentially linear for 6 h. however, the rate of adsorption of dna increased when the dna-polybrene mixture was preincubated for several hours prior to addition to cells. a recombinant plasmid carrying an inducible chloramphenicol acetyl ... | 1986 | 3462005 |
| genetic studies on two carboxylesterase loci in aedes albopictus. | two esterase loci, est-4 and est-5, in aedes albopictus encode carboxylesterases in 4th-instar larvae, pupae and adults. the electrophoretic bands migrate on agar gels toward the most anodal side, those of est-5 followed by those of est-4. linkage studies on the two loci revealed that they were arranged on linkage group 2 in the following order: est-4-(0.9 +/- 0.7 to 4.3 +/- 1.4 map units)-p(pigmented pupa)-(2 map units, as previously determined)-wb(white-body)-(18.2 +/- 2.6 to 21.0 +/- 1.8 map ... | 1987 | 3504903 |
| estimation of the northern limits of distribution of aedes albopictus in north america. | 1987 | 3504917 | |
| variation in the vector competence of geographic strains of aedes albopictus for dengue 1 virus. | eight geographic strains of aedes albopictus from asia and north america and one north american strain of aedes aegypti were tested for their vector competence with dengue 1 virus. three groups of ae. albopictus were established based on their vector competence: a) the oahu laboratory strain, b) the three malaysian strains, and c) the tokyo and three north american strains. the three north american strains were similar to the strain of ae. aegypti from houston, texas in their ability to transmit ... | 1987 | 3504922 |
| aedes albopictus in chicago, illinois. | 1987 | 3504953 | |
| the discovery and distribution of aedes albopictus in harris county, texas. | 1986 | 3507493 | |
| the identification of aedes albopictus in the nearctic region. | an introduced, breeding population of aedes albopictus has been established in harris county, texas, and several parishes in louisiana. the problem of its identification and separation from the indigenous nearctic mosquito fauna is addressed. using the keys of darsie and ward (1981), the author offers suggested inserts which will accomplish the identification of adult females and larvae in the nearctic region. additional pointers are given for distinguishing albopictus from the 2 common containe ... | 1986 | 3507508 |
| the perspective of aedes albopictus from the administrative viewpoint. | mosquito control administrators should consider a newly introduced species in view of local conditions. aedes albopictus was discovered in harris county while surveying for aedes aegypti. recognizing a potential threat to public health, local and state officials were notified of the discovery. questions posed by the introduction of a foreign species requires a cooperative application of research resources. the centers for disease control (cdc) should serve as the coordinator of this effort. | 1986 | 3507521 |
| the significance of the introduction of aedes albopictus into the southeastern united states with implications for the caribbean, and perspectives of the pan american health organization. | the introduction and apparent infestation of aedes albopictus into the gulf states of texas and louisiana in the united states is viewed as the most singular medical entomological happening of this decade in the americas. the implications for the caribbean and other countries southward are serious, as the habitat described for this stegomyia cousin of ae. aegypti, is amply available in the windward and leeward islands of the antilles. studies from the pacific and regions of southeast asia indica ... | 1986 | 3507522 |
| fusion of semliki forest virus infected aedes albopictus cells at low ph is a fusion from within. | herein, it is shown for the first time that the mechanism of fusion followed in aedes albopictus cells infected with semliki forest virus induced by low ph exposure is a "fusion from within". several parameters were studied disclosing that the development of the fusion capacity of the cells is directly related to the synthesis of viral specific products. these findings were further substantiated by utilizing various chemicals to inhibit viral specific events during infection, protein synthesis a ... | 1986 | 3521544 |
| use of the avidin-biotin-peroxidase complex immunocytochemical procedure for detection of rift valley fever virus in paraffin sections of mosquitoes. | the avidin-biotin-peroxidase complex (abc) immunocytochemical procedure was used to locate rift valley fever (rvf) virus antigen in infected aedes albopictus c6/36 cultured cells and in serial paraffin sections of intrathoracically-injected egyptian culex pipiens. fixation of the cultured cells in formaldehyde or periodate-lysine-paraformaldehyde, and fixation of whole mosquitoes with formaldehyde resulted in good preservation of morphology and excellent differential staining between uninfected ... | 1986 | 3532843 |
| isolation of a new rhabdovirus in australia related to tibrogargan virus. | a virus isolated from the blood of a healthy steer and designated dpp53 was shown to have rhabdovirus morphology. although dpp53 virus was antigenically related to tibrogargan virus by reciprocal immunofluorescence and neutralization tests, the viruses were distinguishable by neutralization tests. dpp53 virus contained rna and was sensitive to both ether and chloroform. the geographical distribution of neutralizing antibody to dpp53 virus in australian cattle corresponded to the distribution of ... | 1986 | 3548679 |
| pleiotropic changes in cycloheximide-resistant insect cell clones. | somatic cell mutants resistant to drugs that interact with the eukaryotic ribosome provide a useful tool for studies on ribosome structure, function, and genetics. from aedes albopictus (mosquito) cells, cycloheximide-resistant mutants (cx-705 and cx-738) that were about 30-fold more resistant to cycloheximide than the parental cells have been obtained. the observation that protein synthesis in cell-free lysates from cx-705 and cx-738 cells was resistant to cycloheximide led us to suspect that t ... | 2013 | 3558252 |
| ribosome metabolism during the vitellogenic cycle of the mosquito, aedes aegypti. | ribosome accumulation and synthesis in the fat body of the mosquito aedes aegypti increased by approx. 4-fold during 18 h after the blood meal, consistent with the pattern of total rna accumulation during the synthetic phase of the vitellogenic cycle. by 24-30 h after the blood meal, the accumulated ribosomes began to be degraded, and the total rna content in the fat body eventually returned to previtellogenic levels. a method has been developed for isolation of ribosomal subunits from fat body, ... | 1987 | 3567223 |
| aedes albopictus in north america: probable introduction in used tires from northern asia. | north american strains of aedes albopictus, an asian mosquito recently introduced into the western hemisphere, exhibit photoperiodic sensitivity and cold-hardiness characteristics similar to strains originating from temperate zone asia. trade statistics for used tire imports, the most likely mode of introduction, also indicate a north asian origin. aedes albopictus, an important vector of dengue and a potential vector of many other arboviral diseases, may therefore have the capability of infesti ... | 1987 | 3576225 |
| a study on susceptibility of indonesia colonies of aedes aegypti and aedes albopictus mosquitoes to experimental infection with dengue type 3 and chikungunya viruses. | 1987 | 3586577 | |
| an outbreak of dengue virus at rio de janeiro--1986. | dengue virus type 1 has been isolated in aedes albopictus cell strain, from sera of patients living in the nova iguaçu county, by rio de janeiro. the clinical picture was characterized by fever, headache, retrobulbar pain, backache, pains in the muscles and the joints and prostration. studies in paired sera confirmed the presence of recent infection by dengue virus type 1. the outbreak reached adjacent areas, including rio de janeiro city (may, 1986). | 1986 | 3587006 |
| semliki forest virus-induced polykaryocyte formation is an atp-dependent event. | infection of aedes albopictus cells with semliki forest virus (sfv) leads to polykaryocyte formation below ph 6.2. this syncytium formation is accompanied by a decrease of the cellular atp level. addition of inhibitors of oxidative phosphorylation leads to a rapid, total depletion of atp in infected cells at ph 6 and results in an inhibition of polykaryocyte formation. however, when cells were exposed for only a few minutes to ph 6 in the presence of the inhibitors and then kept at ph 7.2, the a ... | 1987 | 3592981 |
| intraspecific and interspecific variation in the sequence and abundance of highly repeated dna among mosquitoes of the aedes albopictus subgroup. | interspecific variation in abundance of highly repeated dna sequences has been examined in three species of the aedes scutellaris and three species of the a. albopictus subgroup of the a. scutellaris group. sequences from a population of a. albopictus were hybridised with whole genomic content from other species and strains. copy number estimates were determined by dot-blot hybridisation. variation in sequence abundance between strains of a. albopictus was as great as between it and the other si ... | 1987 | 3597124 |
| multiplication of chikungunya virus in salivary glands of aedes albopictus (oahu strain) mosquitoes: an electron microscopic study. | aedes albopictus as well as aedes aegypti is an important vector of chikungunya and dengue viruses. electron microscopic observations on the salivary glands of ae. albopictus infected with chikungunya virus were performed in comparing with those of ae. aegypti infected with dengue virus. no virus budding from the cell surface of the chikungunya-infected mosquito's salivary glands was found as shown in dengue-infected ones, in contrast to the findings of the mammalian cells such as vero, kb, imr, ... | 1986 | 3599528 |
| a novel method for the detection of early events in cell-cell fusion of semliki forest virus infected cells growing in monolayer cultures. | semliki forest virus infected aedes albopictus cells were used to investigate virus induced cell-cell fusion. it was shown by a novel method that cell-cell fusion was completed within approximately 5 minutes after triggering the fusion event by low ph. this method consists of fixing fusing cells with glutaraldehyde and microinjecting the highly fluorescent and rapidly diffusing dye lucifer yellow. in contrast, polykaryon formation, the usually used criterion to measure cell-cell fusion occurred ... | 1987 | 3606395 |
| susceptibility of aedes albopictus c6/36 cells to viral infection. | the susceptibility of the c6/36 clone of aedes albopictus monolayer cell cultures was determined with 46 prototype viruses passed through three subcultures. viral growth was confirmed by titration of the passage material in other susceptible host systems. nineteen viruses demonstrated good growth in c6/36 cells: coxsackievirus group a type 10 and group b types 2, 3, 4, and 5; enterovirus 69; mumps virus; poliovirus types 1 to 3; reovirus types 1 to 3; vaccinia virus; dengue virus type 2; eastern ... | 1987 | 3611315 |
| sexual transmission of dengue viruses by aedes albopictus. | male aedes albopictus experimentally infected with dengue virus types 1, 2, 3, or 4 transmitted their infection sexually to female ae. albopictus. such transmission was enhanced if the females had taken a bloodmeal 2 to 7 days prior to mating. male ae. albopictus also transmitted dengue virus vertically to their f1 progeny. infected progeny were found among those derived from eggs laid greater than or equal to 73 hr after mating but not among those derived from eggs laid prior to that time. this ... | 1987 | 3661831 |
| in vitro cultivation of rickettsia-like-organisms from glossina spp. | a method is described for the in vitro cultivation of the rickettsia-like-organisms (rlo) from glossina spp. which are believed to be associated with susceptibility to trypanosome infection. cultures of rlo were established by infecting a mosquito cell line (aedes albopictus) with haemolymph taken from teneral flies. rlo from nine species of glossina have been isolated and maintained in continuous culture using this technique. | 1987 | 3662675 |
| inter and intraspecific variation in nuclear dna content in aedes mosquitoes. | haploid nuclear dna of 23 species of aedes, as determined by feulgen cytophotometry, was found to vary 3-fold. this was accompanied by a 2-fold variation in total chromosomal length. there was a significant correlation (r = 0.765, p less than 0.001) between these two parameters. genome size varied from 0.87 pg to 1.3 pg among 10 strains of aedes albopictus, from wide geographic regions. large scale differences in chromosomal dna amounts have accompanied speciation and evolution in aedine mosquit ... | 2013 | 3679880 |
| marituba (bunyaviridae) virus replication in cultured aedes albopictus cells and in l-a9 cells. | the replication of marituba virus (bunyavirus genus, family bunyaviridae), was studied in aedes albopictus (mosquito) cells. infection of aedes albopictus cells with marituba virus was characterized by an initial acute phase of infection in which large amounts of virus were produced and further by a persistent phase of infection in which virus yield was much lower. no changes in host cell dna, rna and protein synthesis were observed in aedes albopictus cells infected with marituba virus. in cont ... | 1986 | 3729731 |
| aedes albopictus, an exotic mosquito vector in the united states. | 1986 | 3740685 | |
| sequence analysis of the e2 gene of a hyperglycosylated, host restricted mutant of sindbis virus and estimation of mutation rate from frequency of revertants. | svap15/21, a strain of sindbis virus (sv) derived from our standard laboratory strain of sv (svstd) after repeated passage on aedes albopictus cells, grows normally in mosquito cells but is host restricted (hr) in vertebrate cells. it is also temperature sensitive (ts) and produces pinpoint plaques on vertebrate cells (sp). e2 glycoprotein of svstd differs from that of the more widely used svhr (from which svstd was derived) by an additional (i.e., third) n-linked glycan. the e2 of svap15/21, in ... | 1986 | 3750843 |
| dominance of the cpe(+) phenotype in hybrid aedes albopictus cells infected with sindbis virus. | the effect of sindbis virus (sv) infection was analyzed in hybrid aedes albopictus cells formed by fusing ouabain-resistant cpe(+) cells to cpe(-) alpha-amanitin resistant cells. although the 24-h yields of virus from the parental cpe(+) and cpe(-) clones were similar, the rates of viral rna synthesis and virus release at early times post-infection were higher in the cpe(+) cells. in all eight hybrid clones studied, the cpe(+) phenotype was dominant. in addition, the kinetics of viral rna synthe ... | 1986 | 3765819 |
| aedes albopictus, yellow fever, and the americas. | 1987 | 3800201 | |
| nucleoside and nucleobase transport and metabolism in wild type and nucleoside transport-deficient aedes albopictus cells. | nucleoside and nucleobase transport and metabolism were measured in atp-depleted and normal aedes albopictus mosquito cells (line c-7-10) by rapid kinetic techniques. the cells possess a facilitated diffusion system for nucleosides, which in its broad substrate specificity and kinetic properties resembles that present in many types of mammalian cells. the michaelis-menten constant for uridine transport at 28 degrees c is about 180 microm. however, the nucleoside transporter of the mosquito cells ... | 1987 | 3814594 |
| detection of chikungunya virus antigen in aedes albopictus mosquitoes by enzyme-linked immunosorbent assay. | double-antibody sandwich and modified sandwich systems of enzyme-linked immunosorbent assay for detecting chikungunya virus antigen present in female mosquitoes, aedes albopictus (oahu strain), were evaluated as simple and rapid methods of selection of a highly susceptible mosquito line. both assays were capable of detecting 3.9 x 10(1) ng (4.0 x 10(6) pfu) or more of the purified antigen. an inhibition system was less sensitive, and a direct system with adsorption of test specimens on the solid ... | 1985 | 3833871 |
| transient expression of the chloramphenicol acetyltransferase gene in cultured mosquito cells. | a recombinant plasmid in which the bacterial chloramphenicol acetyltransferase (cat) gene is under the control of the drosophila heat-shock protein (hsp) 70 promoter has been introduced into cultured mosquito (aedes albopictus) cells using 1,5-dimethyl-1,5-diazaundecamethylene polymethobromide (polybrene) and dimethylsulfoxide (dmso). cat activity was induced by incubating transfected cells at 37 degrees c, and high levels of enzyme activity were maintained for more than 24 h after the temperatu ... | 1985 | 3864716 |
| characterization of the ribosomal proteins from mosquito (aedes albopictus) cells. | proteins from the large and small subunits of aedes albopictus (mosquito) cytoplasmic ribosomes were characterized by two-dimensional polyacrylamide gel electrophoresis. the small subunit contained 28-31 proteins ranging in molecular mass from 10 to 49 kda. the large subunit contained 36-39 proteins that ranged in molecular mass from 11 to 53 kda. the largest protein on the small subunit, s1, was the predominant phosphorylated ribosomal protein. under long-term labelling conditions, l4 and l33 w ... | 1985 | 3926499 |
| titer distribution analysis of chikungunya virus in aedes albopictus (diptera: culicidae). | 1986 | 3950931 | |
| exclusion of superinfecting homologous virus by sindbis virus-infected aedes albopictus (mosquito) cells. | the infection of tissue-cultured aedes albopictus (mosquito) cells by an alphavirus ultimately results in a persistently infected cell population which can be maintained in the laboratory for years. one characteristic of this culture is that it will not support the replication of superinfecting homologous virus. we have previously shown that mosquito cells persistently infected with sindbis virus produce an antiviral agent which when applied to uninfected mosquito cells suppresses sindbis virus ... | 1986 | 3951024 |
| persistent infection of aedes albopictus c6/36 cells by bunyamwera virus. | two cell lines persistently infected with bunyamwera virus have been established from the c6/36 clone of aedes albopictus cells. the cells express bunyamwera virus antigens as detected by immunofluorescence and are resistant to superinfection with bunyamwera virus and other bunyaviruses, but not dugbe virus (nairovirus) nor vesicular stomatitis virus. the virus released from the persistently infected cells developed an altered cloudy or "bull's-eye" plaque morphology with increasing passage leve ... | 1986 | 3952984 |
| replication pattern of double minutes derived from an insect cell line. | the dna replication pattern of double minutes derived from an established cell line of aedes albopictus is described. although the vast majority of double minutes replicate semiconservatively once during the s phase, some double minutes appear to exhibit different pattern(s). two theories are suggested as possible explanations of our findings. | 1985 | 3967745 |
| synthesis of proteins and glycoproteins in dengue type 2 virus-infected vero and aedes albopictus cells. | fifteen proteins were detected in vero cells infected by dengue type 2 (den-2) virus that were not observed in mock-infected cells, namely p98, p82, p67, gp60, gp54, gp46, p30, p28, gp22, gp20, p18, gp16, p15, p14 and gp13. with the exceptions of gp54 and gp13, polypeptides corresponding to those listed above were also observed in den-2 virus-infected aedes albopictus c6/36 cells. pulse-chase labelling experiments suggested a possible precursor-product relationship between p30 and p28, and betwe ... | 1985 | 3973563 |
| failure to detect natural transovarial transmission of dengue viruses by aedes aegypti and aedes albopictus (diptera: culicidae). | 1985 | 4009622 | |
| [controlled-release tubes containing phoxim in killing larvae of aedes albopictus]. | 1985 | 4028153 | |
| growth of epizootic hemorrhagic disease, akabane, and ephemeral fever viruses in aedes albopictus cells maintained at various temperatures. | the growth curves of one epizootic hemorrhagic disease (ehd) virus serotype (reoviridae), two akabane virus strains (bunyaviridae) and three bovine ephemeral fever (bef) group viruses (rhabdoviridae) were determined in aedes albopictus cells maintained at 15, 20, 28 and 33 degrees c. ae albopictus cells supported the growth of all the viruses although not necessarily at all temperatures. because none of the viruses exhibited cytopathic effect in ae albopictus cells, growth was assayed in baby ha ... | 1985 | 4051908 |
| conformational changes at ph 6 on the cell surface of semliki forest virus-infected aedes albopictus cells. | the mechanism of semliki forest virus-induced fusion from within at low ph was studied in aedes albopictus cells. the fusion was found to occur in at least two steps, namely, a fast initial step which is ph dependent and temperature independent, and a second slower process which is ph independent and temperature dependent. the initiation step induced by low ph exposure constitutes an irreversible conformational change of a protein probably of viral origin located at the cell surface. | 1985 | 4060582 |
| sindbis virus mutants able to replicate in methionine-deprived aedes albopictus cells. | previous work from this laboratory has shown that the replication of sindbis virus (sv) in aedes albopictus cells is sensitive to methionine withdrawal. this sensitivity is thought to reflect a diminished concentration of s-adenosylmethionine (ado met) resulting from methionine starvation. serial passage of sv on ae. albopictus cells maintained in low concentrations of methionine gave rise to a population of mutants whose replication in mosquito cells was resistant to methionine starvation. in v ... | 1985 | 4060595 |
| protein synthesis in cells infected by chilo iridescent virus (iridovirus, type 6). | synthesis of the polypeptides induced in civ-infected cells was studied using radiolabelled methionine in a permissive cell line of choristoneura fumiferana. analysis of labelled cell extracts by high resolution polyacrylamide gel electrophoresis (page) revealed the sequential appearance of at least 28 structural and non-structural polypeptides in permissive conditions. this was confirmed by analysis of protein synthesis in non-permissive cell lines of lymantria dispar, aedes albopictus or by th ... | 1985 | 4062562 |
| expression of recombinant vaccinia virus-derived alphavirus proteins in mosquito cells. | a recombinant vaccinia virus strain which contains and expresses a 26s cdna insert encoding sindbis virus structural proteins (vv:3s) was used to infect a continuous line of aedes albopictus mosquito cells. there were not visible cytopathic effects due to the virus infection and the cells continued to grow normally. however, examination of the proteins present in the cytoplasm of the infected cells with sindbis virus-specific antisera revealed that sindbis virus proteins were being synthesized a ... | 1985 | 4067584 |
| two monoclonal antibodies against la crosse virus show host-dependent neutralizing activity. | mammalian and arthropod cell cultures were used to assess the neutralizing activity of six monoclonal antibodies specific for the g1 glycoprotein of la crosse virus. four antibodies, two neutralizing and two non-neutralizing, showed no host-dependent differences, giving similar results when post-treatment infectivity was determined using either aedes albopictus cells or bhk-21 cells. for two other antibodies, however, dissimilar activities were observed between the vertebrate and invertebrate ce ... | 1985 | 4067586 |
| one-step separation of the components of semliki forest virus by cation exchange chromatography. | although several procedures for isolating viral proteins have been described, the simultaneous separation of all the viral macromolecules in a single step has not yet been reported. we now describe tua (triton x-100, urea, acetic acid, ph 4.2)-sp (sulphopropyl)-trisacryl cation exchange chromatography, which proved to be ideal for this purpose. optimal conditions for chromatography were established by screening on tua-page (polyacrylamide gel electrophoresis) using a horizontal linear 0-8.5 m ur ... | 1985 | 4077952 |
| studies on the pathogenesis of bovine ephemeral fever in sentinel cattle. i. virology and serology. | twenty-two sentinel cattle were observed daily during an outbreak of ephemeral fever on a dairy farm in eastern australia in the summer of 1981-82. of the 22 cattle, 9 developed clinical ephemeral fever. none developed sub-clinical infection. the pattern of the epidemic was a single index case followed 10 days later by the main epidemic wave which lasted for 7 days. this wave stopped when there were still 14 uninfected susceptible animals remaining in the sentinel group, and when biting flies we ... | 1985 | 4095897 |
| morphological variants of sindbis virus obtained from infected mosquito tissue culture cells. | tissue-cultured aedes albopictus cells infected with morphologically homogeneous sindbis virus were found to produce progeny virions which could be divided into three classes based on size. the thickness of the envelope was constant on all three sizes of progeny virions suggesting that the variability in size rested with the viral nucleocapsid. it is suggested that the three classes of virions have icosahedral nucleocapsids composed of common subunits organized in decreasing triangulation number ... | 1973 | 4128381 |
| host-induced modification of dengue-2 virus surface antigens in aedes albopictus cells. | 1973 | 4132537 | |
| susceptibility of aedes albopictus mosquito cell line to some arboviruses. | 1973 | 4147144 |