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a remarkable, precisely timed release of hyperglycemic hormone from endocrine cells in the gut is associated with ecdysis in the crab carcinus maenas.molting or ecdysis is the most fundamentally important process in arthropod life history, because shedding of the exoskeleton is an absolute prerequisite for growth and metamorphosis. although the hormonal mechanisms driving ecdysis in insects have been studied extensively, nothing is known about these processes in crustaceans. during late premolt and during ecdysis in the crab carcinus maenas, we observed a precise and reproducible surge in hemolymph hyperglycemic hormone (chh) levels, which wa ...199910557280
ecdysis of decapod crustaceans is associated with a dramatic release of crustacean cardioactive peptide into the haemolymph.on the basis of detailed analyses of morphological characteristics and behavioural events associated with ecdysis in a crab (carcinus maenas) and a crayfish (orconectes limosus), a comprehensive substaging system has been introduced for the ecdysis stage of the moult cycle of these decapod crustaceans. in a remarkably similar stereotyped ecdysis sequence in both species, a passive phase of water uptake starting with bulging and rupture of thoracoabdominal exoskeletal junctions is followed by an ...200010637181
agonistic behaviour and biogenic amines in shore crabs carcinus maenas.to investigate the role of certain neurohormones in agonistic behaviour, fights were staged between pairs of size-matched male shore crabs carcinus maenas, and blood samples were taken immediately after the contests had been resolved. samples were also taken from these crabs at rest (before and after fighting) and after walking on a treadmill. a control group of crabs also had samples taken on each experimental day. concentrations of tyramine, dopamine, octopamine, serotonin (5-ht) and norepinep ...200010637182
immunochemical localisation of proliferating cells in mussel digestive gland tissue.the distribution of proliferating cells in the digestive gland of the common marine mussel, mytilus galloprovincialis lmk, was investigated by means of immunochemical techniques employing pc10, a commercial monoclonal antibody to the proliferating cell nuclear antigen (pcna). immunoblot analysis of digestive gland whole homogenates revealed a single crossreactive band of 36-37 kda, identical to the corresponding protein of rat liver and murine melanoma cells. a band of slightly higher electropho ...199910661321
cadmium influx and efflux across perfused gills of the shore crab, carcinus maenas.cadmium influx across perfused gills of the shore crab carcinus maenas exposed to 9.0 µm cadmium in the external medium ranged from 0.6 to 2.0 nmol cd(2+) g(-1) gill wet wt. per h. cadmium efflux across perfused gills exposed to 9.0 µm cadmium in the internal medium ranged from 0.3 to 0.9 nmol cd(2+) g(-1) gill wet wt. per h. there was no significant difference between cadmium influx and efflux across the gills. cadmium influx across the gills was not detectable after exposure to lanthanum in th ...200010686328
changes in metallothionein concentrations in response to variation in natural factors (salinity, sex, weight) and metal contamination in crabs from a metal-rich estuary.intermoult male and female crabs pachygrapsus marmoratus and carcinus maenas were sampled from three sites between the mouth and 25 km upstream in the gironde, the most cd-contaminated estuary in france, in order to study the relative importance of natural factors (salinity, sex, weight) and accumulated metal concentrations on metallothionein (mt) concentrations. in the two species studied, higher metal, total protein and mt concentrations were observed in the hepatopancreas than in the gills. i ...200010713280
the ability to feed in hypoxia follows a seasonally dependent pattern in shore crab carcinus maenas.the ability of the adult shore crab carcinus maenas, native to the bay of arcachon (sw france), to feed in hypoxia was determined at various seasons. crabs previously kept at field temperature were fed after a 5-day fasting period at 15 degrees c. their blood oxygenation and ph regulation strategy and also their gill anatomy were analysed. from may to october, c. maenas feed at levels of o(2) partial pressure (p(o(2))) in the water, pwo(2)=2 kpa (1 mg l(-1)), without switching to their anaerobic ...200010727691
haemolymph mg(2+) regulation in decapod crustaceans: physiological correlates and ecological consequences in polar areas.reptant decapod crustaceans are almost absent from the southern ocean south of the antarctic convergence. we tested the hypothesis that this may be due to the reduced ability of this group to regulate mg(2+) levels in the haemolymph ([mg(2+)](hl)). mg(2+) acts as an anaesthetic in marine invertebrates and its level is higher in reptantia (crabs such as cancer spp., chionoecetes spp., maja spp., 30-50 mmol l(-)(1)) than in natantia (prawns such as pandalus spp., palaemon spp., crangon spp., 5-12 ...200010729286
identification of orcokinin gene-related peptides in the brain of the crayfish procambarus clarkii by the combination of maldi-tof and on-line capillary hplc/q-tof mass spectrometries and molecular cloning.we developed a strategy for the exploration of brain peptides in the red swamp crayfish, procambarus clarkii, utilizing the combined techniques of matrix-assisted laser desorption/ionization with time-of-flight mass spectrometry (maldi-tof ms), molecular cloning, and on-line capillary reversed-phase hplc/quadrupole orthogonal acceleration time-of-flight (q-tof)-ms. we initially performed direct maldi-tof ms analysis with slices of the brain. the ms spectra from a slice of the olfactory lobe indi ...200010753578
estimating the energetic cost of fighting in shore crabs by noninvasive monitoring of heartbeat rate.after establishing shore crabs, carcinus maenas, individually in separate aquaria, we used a noninvasive infrared phototransducer to monitor their heartbeat rate continously before, during and after fights with intruder crabs. we confirmed that heartbeat rate is a reliable indicator of oxygen consumption and then used it to estimate indirectly the energetic cost of fights differing in duration and intensity, and its dependence on prior residence and relative size of opponent. prior residence in ...200010792926
endocrine cells in the gut of the shore crab carcinus maenas immunoreactive to crustacean hyperglycaemic hormone and its precursor-related peptide.the distribution and morphology of gut endocrine cells, which are immunoreactive to crustacean hyperglycaemic hormone (chh) and the corresponding precursor-related peptide (cprp), have been described in the shore crab carcinus maenas. the cells are uniquely distributed throughout the fore- and hindgut, but were never observed in the midgut or associated caeca. expression of chh and cprp in the gut endocrine cells is generally restricted to premoult, although small numbers of immunoreactive cells ...200010805088
expression of arginine kinase enzymatic activity and mrna in gills of the euryhaline crabs carcinus maenas and callinectes sapidus.phosphagen kinases catalyze the reversible dephosphorylation of guanidino phosphagens such as phosphocreatine and phosphoarginine, contributing to the restoration of adenosine triphosphate concentrations in cells experiencing high and variable demands on their reserves of high-energy phosphates. the major invertebrate phosphagen kinase, arginine kinase, is expressed in the gills of two species of euryhaline crabs, the blue crab callinectes sapidus and the shore crab carcinus maenas, in which ene ...200010903154
phenotypic clines, plasticity, and morphological trade-offs in an intertidal snail.understanding the genetic and environmental bases of phenotypic variation and how they covary on local and broad geographic scales is an important goal of evolutionary ecology. such information can shed light on how organisms adapt to different and changing environments and how life-history trade-offs arise. surveys of phenotypic variation in 25 littorina obtusata populations across an approximately 400-km latitudinal gradient in the gulf of maine revealed pronounced clines. the shells of snails ...200010937192
observations on the interaction of zinc and cadmium uptake rates in crustaceans (amphipods and crabs) from coastal sites in uk and france differentially enriched with trace metals.this paper presents results on the possible interaction of zinc and cadmium uptake rates in crustaceans. zn and cd uptake rates were measured in amphipods (orchestia gammarellus) and crabs (carcinus maenas and pachygrapsus marmoratus) from five coastal sites in britain and france subjected to different degrees of trace metal enrichment. the presence or absence of 100 µg l(-1) of one metal (1.53 µm l(-1) zn, 0.89 µm l(-1) cd) had an inconsistent effect on the rate of uptake of the other metal by ...200010958954
involvement of crustacean hyperglycemic hormone in the control of gill ion transport in the crab pachygrapsus marmoratus.total extracts of sinus glands (sg) of the euryhaline grapsid crab pachygrapsus marmoratus contain peptidic factor(s) that stimulate osmoregulatory processes in isolated and perfused posterior gills from crabs acclimated to dilute seawater. this study investigated the nature of the active factor(s). separation of p. marmoratus sg peptides by reverse-phase hplc, followed by a direct enzyme-linked immunosorbent assay using an anti-carcinus maenas crustacean hyperglycemic hormone (chh) antiserum, i ...200011017781
developing the options for managing marine pests: specificity trials on the parasitic castrator, sacculina carcini, against the european crab, carcinus maenas, and related species.the impacts of introduced marine pests are becoming increasingly apparent, prompting interest in the possibility of their biological control. we undertook laboratory and field experiments on host selection of one potential control agent (the endoparasitic barnacle, sacculina carcini) against its natural host (the widely invasive european shore crab, carcinus maenas) and several confamilial and more distantly related crustaceans. for comparison, we also tested host specificity in a related parasi ...200011058725
isolation and characterisation of five neurotoxic and cardiotoxic polypeptides from the sea anemone anthopleura elegantissima.five toxins (ape 1 to ape 5) of the sea anemone species anthopleura elegantissima (brandt) have been isolated from a toxic by-product fraction of its concentrated crude watery-methanolic extract, prepared previously for the isolation of a neuropeptide (the head-activator) by schaller and bodenmüller (proc. natl. acad. sci. usa 78 (1981) 7000) from 200kg sea anemones. toxin purification was performed by desalting of the starting material by dialysis (mwco 3500) against distilled water, anion exch ...200111072049
molecular characterization of v-type h(+)-atpase (b-subunit) in gills of euryhaline crabs and its physiological role in osmoregulatory ion uptake.the vacuolar-type h(+)-atpase (v-atpase) has been implicated in osmoregulatory ion uptake across external epithelia of a growing variety of species adapted to life in fresh water. in the present study, we investigated whether the v-atpase may also function in a euryhaline species that tolerates brackish water (8 salinity) but not fresh water, the shore crab carcinus maenas. cdna coding for the regulatory b-subunit of the v-atpase was amplified and sequenced from c. maenas gills and partially seq ...200111104708
epizootiology of the parasitic dinoflagellate hematodinium sp. in the american blue crab callinectes sapidus.hematodinium sp. is a parasitic dinoflagellate that infects and kills blue crabs callinectes sapidus. periodic outbreaks of dinoflagellate infections with subsequent high host mortalities prompted a study of the epizootiology and distribution of the crab pathogen. hemolymph samples from over 13000 crabs were assessed for infections over 8 yr. moderate to high prevalences were found at several locations along the atlantic and gulf coasts of the united states. in the coastal bays of maryland and v ...200011145454
immunolocalization of four antioxidant enzymes in digestive glands of mollusks and crustaceans and fish liver.the aim of this work was to determine the immunolocalization of the antioxidant enzymes catalase, cu,zn-superoxide dismutase (sod), mn-sod, and glutathione peroxidase (gpx) in the bivalve mollusks mytilus galloprovincialis and crassostrea sp., the crab carcinus maenas, and the teleostean fish mugil cephalus. by immunoblotting, crossreactivity between antibodies and the corresponding proteins in the digestive gland/hepatopancreas of invertebrates and the fish liver was demonstrated. immunohistoch ...200011151409
hemolymph levels of methyl farnesoate increase in response to osmotic stress in the green crab, carcinus maenas.the salinity of estuarine environments can vary widely, exposing resident organisms to considerable osmotic stress. the green crab carcinus maenas is well known for its ability to osmoregulate in response to such stress. therefore, we tested the relationship between osmoregulation and hemolymph levels of methyl farnesoate (mf), a compound previously shown to rise in response to various types of environmental stresses. when crabs were transferred from 100% seawater to dilute (hypo-osmotic) seawat ...200111223391
the competitive and predatory impacts of the nonindigenous crab carcinus maenas (l.) on early benthic phase dungeness crab cancer magister dana.we evaluate the potential competitive and predatory impacts of nonindigenous european green crab carcinus maenas on native dungeness crab cancer magister in the northeast pacific. the coastal estuaries of washington state, usa, provide appropriate habitat for recently introduced green crab, yet these areas are important nursery grounds for dungeness crab and contribute greatly to the coastal crab fishery. juvenile dungeness crabs are dependent on limited intertidal epibenthic shell for refuge ha ...200111239624
protein synthesis and specific dynamic action in crustaceans: effects of temperature.temperature influences the specific dynamic action (sda), or rise in oxygen uptake rate after feeding, in eurythermal and stenothermal crustaceans by changing the timing and the magnitude of the response. intra-specific studies on the eurythermal crab, carcinus maenas, show that a reduction in acclimation temperature is associated with a decrease in sda magnitude, resulting from an increase in sda duration but a decrease in peak factorial scope (the factorial rise in peak sda over prefeeding val ...200111246047
crustacean hyperglycaemic hormone (chh)-like peptides and chh-precursor-related peptides from pericardial organ neurosecretory cells in the shore crab, carcinus maenas, are putatively spliced and modified products of multiple genes.about 24 intrinsic neurosecretory neurons within the pericardial organs (pos) of the crab carcinus maenas produce a novel crustacean hyperglycaemic hormone (chh)-like peptide (po-chh) and two chh-precursor-related peptides (po-cprp i and ii) as identified immunochemically and by peptide chemistry. edman sequencing and ms revealed po-chh as a 73 amino acid peptide (8630 da) with a free c-terminus. po-chh and sinus gland chh (sg-chh) share an identical n-terminal sequence, positions 1-40, but the ...200111336648
rubidium uptake is increased in shore crabs, carcinus maenas, acclimated to dilute seawater.in dilute seawater, carcinus maenas hyperosmoregulates by actively absorbing na, k, and cl. here we characterize k uptake using a novel technique. rb was used as a tracer for k transport, and hemolymph rb levels were measured using cation chromatography. hemolymph rb was detectable at 0.1 mmol l(-1), which enabled determination of initial rate of rb uptake. crabs maintained for 3 wk in dilute artificial seawater (35% asw crabs) maintained na and k above the level of the external media and had el ...201311517457
isolation, sequence analysis, and physiological properties of enkephalins in the nervous tissue of the shore crab carcinus maenas l.[leu]- and [met]enkephalin from thoracic ganglia of the shore crab carcinus maenas have been purified to homogeneity by a reversed-phase hplc procedure. automated gas-phase sequencing revealed a primary structure identical with that of enkephalins from vertebrates. the immunoreactive material in different parts of the nervous system of c. maenas was measured by ria. isolated crustacean [leu]enkephalin shows physiological properties identical to synthetic [leu]enkephalin in our bioassay. the enke ...199111607217
nitrite reductase activity of deoxy carcinus maenas hemocyanin: formation of the half-met derivative. 199611666340
characterization of cdna encoding molt-inhibiting hormone of the crab, cancer pagurus; expression of mih in non-x-organ tissues.synthesis of ecdysteroids (molting hormones) by crustacean y-organs is regulated by a neuropeptide, molt-inhibiting hormone (mih), produced in eyestalk neural ganglia. we report here the molecular cloning of a cdna encoding mih of the edible crab, cancer pagurus. full-length mih cdna was obtained by using reverse transcription-polymerase chain reaction (rt-pcr) with degenerate oligonucleotides based upon the amino acid sequence of mih, in conjunction with 5'- and 3'-race. full-length clones of m ...200111707332
identification and ultrastructural characterization of a novel virus from fish.during routine investigations on fish, a virus (isolate df 24/00) with novel morphological features and hitherto undescribed morphogenesis was isolated from a white bream (blicca bjoerkna l.; teleostei, order cypriniformes). cell-free virions consist of a rod-shaped nucleocapsid (120-150x19-22 nm) similar to that seen in baculoviruses. the virion has a bacilliform shape (170-200x75-88 nm) reminiscent of rhabdoviruses with an envelope containing coronavirus-like spikes (20-25 nm). df 24/00 replic ...200111714959
neurogenesis in the central olfactory pathway of the adult shore crab carcinus maenas is controlled by sensory afferents.the number of olfactory projection neurons (opns) in the brain of the juvenile and adult shore crab carcinus maenas continues to increase during the life of the animal. in vivo labeling of adult crabs with the proliferation marker bromodeoxyuridine (brdu) revealed a group of proliferating neuronal precursor cells in the lateral soma clusters (lcs) and in the soma clusters of the hemiellipsoid bodies (hbcs). the lcs contain the cell bodies of the olfactory projection neurons and the hbcs house th ...200111745646
expression of crustacean (callinectes sapidus) molt-inhibiting hormone in insect cells using recombinant baculovirus.molt-inhibiting hormone (mih) negatively regulates the synthesis of ecdysteroid molting hormones by crustacean y-organs. we report here the expression of blue crab (callinectes sapidus) mih in insect cells using recombinant baculovirus. insect sf9 cells were transfected with recombinant baculovirus containing a dna insert encoding the c. sapidus mih prohormone (signal sequence plus mature hormone). the construct was designed to yield a mature, fully processed recombinant mih (recmih). several ba ...200211754021
glycoproteins from the cuticle of the atlantic shore crab carcinus maenas: i. electrophoresis and western-blot analysis by use of lectins.the protein and glycoprotein content of four different neutral or acidic solvent extracts (0.5 m kcl, 10% edta, 0.1 n hcl, or 2% acetic acid) from the mineralized exoskeleton of a decapod crustacean, the atlantic shore crab carcinus maenas, were characterized by quantitative analysis of proteins, sds-page analysis, and probing with lectins on blots. the lectins used were conconavalin a, jacalin, soybean agglutinin, maackia amurensis agglutinin ii, and sambucus nigra agglutinin. the results show ...200211842016
ion transport across posterior gills of hyperosmoregulating shore crabs (carcinus maenas): amiloride blocks the cuticular na(+) conductance and induces current-noise.split gill lamellae and gill cuticles of shore crabs (carcinus maenas) adapted to 10 per thousand salinity were mounted in a modified ussing-type chamber. with nacl saline on both sides, split gill lamellae generated a short-circuit current (i(sc)) of -301+/-16 microa cm(-2) at a conductance (g(te)) of 40+/-2 ms cm(-2). the net influxes of na(+) and cl(-) were 8.3+/-2.6 and 18.2+/-2.7 micromol cm(-2) h(-1), respectively. external amiloride (100 micromol l(-1)) reduced g(te) to approximately 50 % ...200211893766
cloning of metallotionein cdnas and its gene in shore crab(carcinus maenas).metallothioneins are cysteine-rich proteins which chelate metal ion(zn, cu, hg and cd)and regulate ion concentrations in animal body. based on the published amino acid sequence of c.maenas metallothionein, completely degenerated pcr primers were designed and two different types of cdna were amplified from gill tissue rna of c.maenas using rt-pcr method. these cdnas were cloned to pgem t easy vector and the nucleotic acid sequences were determined. the results showed that one group of the cdna se ...200012058179
antioxidant enzymes and peroxisome proliferation in relation to contaminant body burdens of pahs and pcbs in bivalve molluscs, crabs and fish from the urdaibai and plentzia estuaries (bay of biscay).with the aim of studying levels of antioxidant and peroxisomal enzymes and the structure of peroxisomes in relation to body burdens of polycyclic aromatic hydrocarbons (pahs) and polychlorinated biphenyls (pcbs), mussels mytilus galloprovincialis, oysters crassostrea sp., crabs carcinus maenas and mullets mugil cephalus were sampled in two basque estuaries (bay of biscay): urdaibai (laida, txatxarramendi, arteaga, and downstream a sewage treatment plant-stp) and plentzia. in general, animals sho ...200212062156
first confirmation of human diarrhoeic poisonings by okadaic acid esters after ingestion of razor clams (solen marginatus) and green crabs (carcinus maenas) in aveiro lagoon, portugal and detection of okadaic acid esters in phytoplankton.a new outbreak of human diarrhoeic poisonings (dsp) with esters of okadaic acid (oa) was confirmed after ingestion of razor clams (solen marginatus) harvested at aveiro lagoon (nw portugal) in the summer of 2001. accumulation of marine toxins in second order consumers was investigated in the edible parts of a shellfish predator abundant at aveiro lagoon, the green crab carcinus maenas. okadaic acid was found, also in a predominant esterified form. levels in edible parts (comprising mainly viscer ...200212076653
gamma-aminobutyric acid-inhibitory motor innervation of leg muscles of the shore crab.the inhibitory motor innervation of a crustacean leg was studied in the crab, carcinus maenas. in in vitro preparations of the central nervous system and the proximal leg nerves, motor nerve recordings demonstrate the presence of a single common inhibitory motor neuron which elicits picrotoxin-sensitive inhibitory junction potentials in a distal leg muscle, the accessory flexor. this inhibitor is the common inhibitor (ci). immunohistochemical detection of the inhibitory motor neuron neurotransmi ...199012106056
differential induction of branchial carbonic anhydrase and na(+)/k(+) atpase activity in the euryhaline crab, carcinus maenas, in response to low salinity exposure.the time course of induction of activity of carbonic anhydrase (ca) and na/k atpase, two enzymes that are central to osmotic and ionic regulation in the eyryhaline green crab, carcinus maenas, was measured in response to a transfer from 32 to 10 ppt salinity. ca activity was low in all gills in crabs acclimated to high salinity. activity was induced in the posterior three gills (g6-g9) starting at 96 hr following transfer to low salinity, with activity peaking at seven post-transfer. na/k atpase ...200212115925
active ammonia excretion across the gills of the green shore crab carcinus maenas: participation of na(+)/k(+)-atpase, v-type h(+)-atpase and functional microtubules.although aquatic animals are generally believed to export nitrogenous waste by diffusion of nh(3) or nh(4)(+) across external epithelia, evidence for active ammonia excretion has been found in a number of species. in the euryhaline green shore crab carcinus maenas, active excretion of ammonia across isolated gills is reduced by inhibitors of the na(+)/k(+)-atpase and vacuolar-type h(+)-atpase. in addition, a functional dynamic microtubule network is necessary, since application of colchicine, ta ...200212177142
cypermethrin induces glutathione s-transferase activity in the shore crab, carcinus maenas.cypermethrin is a synthetic pyrethroid that is particularly toxic to crustacea. it is therefore applied as a chemotherapeutant in the salmonid aquaculture industry for the treatment of sea lice infestations. after use, cypermethrin is released directly into the marine environment, to be diluted by fresh seawater. the shore crab, carcinus maenas is found in the vicinity of fish farms, and may come into contact with released cypermethrin. the detoxification enzyme, glutathione s-transferase (gst) ...200212206409
specific dynamic action in the shore crab, carcinus maenas (l.), in relation to acclimation temperature and to the onset of the emersion response.the rate of oxygen uptake (mo(2)) of shore crabs following a period of fasting varied directly with acclimation temperature, with a q(10) of 2.96 between 7 degrees and 15 degrees c and a q(10) of 2.11 between 15 degrees and 22 degrees c. the factorial rise in mo(2) following a meal (specific dynamic action [sda]) ranged between 1.9 and 3.1 and varied with temperature, being highest at 15 degrees c and significantly lower at both 7 degrees and 22 degrees c, despite similar ration sizes in all gro ...201312324891
a non-destructive assessment of the exposure of crabs to pah using elisa analyses of their urine and haemolymph.urine and haemolymph can be repeatedly sampled from crabs with no (or limited) damage to the organism. their analysis offers a measure of the animals' exposure to biologically available contaminants. shore crabs (carcinus maenas) were exposed to the pahs phenanthrene and pyrene at concentrations ranging from 20 to 200 microg l(-1). after 48 h, urine and haemolymph samples were taken and analysed using elisa and uv-fluorescence spectrophotometry. high correlations were recorded between the two se ...201212408657
inhibition of carbonic anhydrase in the gills of two euryhaline crabs, callinectes sapidus and carcinus maenas, by heavy metals.two subcellular fractions of gill tissue, cytoplasm and basolateral membranes, from two species of euryhaline decapod crustaceans, callinectes sapidus and carcinus maenas, acclimated to low salinity, were isolated via differential centrifugation. carbonic anhydrase activity from both fractions was titrated against a variety of heavy metals in vitro. the metals ag(+), cd(2+), cu(2+) and zn(+) showed inhibitory action against the enzyme. ki values for these metals against cytoplasmic ca from c. sa ...200212458188
influence of oxygen partial pressures on protein synthesis in feeding crabs.many water-breathing animals have a strategy that consists of maintaining low blood po2 values in a large range of water oxygenation level (4-40 kpa). this study examines the postprandial changes in o2 consumption, arterial blood po2, and tissue protein synthesis in the shore crab carcinus maenas in normoxic, o2-depleted, and o2-enriched waters to study the effects of this strategy on the o2 consumption and peptide bond formation after feeding. in normoxic water (21 kpa), the arterial po2 was 1. ...200312529287
female sex pheromone-mediated effects on behavior and consequences of male competition in the shore crab (carcinus maenas).exposure to receptive female pheromone elicits guarding behavior in shore crab males (carcinus maenas), but little is known about the effects of sex pheromone on male competition or if the female plays an active role in mate choice. this study examined whether female pheromone enhanced agonistic behavior between males and what effects visual and chemical cues had on the rules and costs of such contests. we also investigated whether females exhibit a preference for males in terms of size. under l ...200312647853
comparative esi-ms study of approximately 2.2 mda native hemocyanins from deep-sea and shore crabs: from protein oligomeric state to biotope.in the past years, the potential of electrospray ionization mass spectrometry (esi-ms) for the observation of intact weak interactions, such as non-covalent protein-ligand, protein-protein, protein-dna complexes, has spread out. the coupling of esi with time-of-flight (tof) and quadrupole-time-of-flight (q-tof) analyzers has even enabled the detection of larger complexes with molecular weights greatly higher than 200 kda. in this paper, we report a comparative esi-ms study on the protein quatern ...200312745211
moult cycle-related changes in biological activity of moult-inhibiting hormone (mih) and crustacean hyperglycaemic hormone (chh) in the crab, carcinus maenas. from target to transcript.the currently accepted model of moult control in crustaceans relies entirely on the hypothesis that moult-inhibiting hormone (mih) and crustacean hyperglycaemic hormone (chh) repress ecdysteroid synthesis of the target tissue (y-organ) only during intermoult, and that changes in synthesis and/or release of these neurohormones are central to moult control. to further refine this model, we investigated the biological activities of these neuropeptides in the crab carcinus maenas, at the target tiss ...200312869204
effects of water activity on oxygen-binding in high-molecular weight, extracellular invertebrate hemoglobin and hemocyanin.we have investigated the effects of water activity on oxygen-binding properties of giant invertebrate oxygen-binding proteins: hemocyanins from the intertidal crab, carcinus maenas and the terrestrial snail, arion ater and hemoglobin from the marine polychaete, arenicola marina, using the osmotic stress method. we show that in contrast to the water-sensitive dimeric or tetrameric hemoglobins from humans, fish and cyclostomes, changes in water activity exert small effects on the oxygen affinity o ...200312941641
exposure patterns of perfluorooctane sulfonate in aquatic invertebrates from the western scheldt estuary and the southern north sea.over the past decades little research has been conducted on the environmental behavior and effects of fluorinated organochemicals (focs). recently it has been reported that perfluorooctane sulfonic acid (pfos) is occurring worldwide. little is known about the pfos levels in organisms originating from the southern north sea and the western scheldt estuary. in this study, we determined, for the first time, the pfos-exposure levels in crangon crangon, carcinus maenas, and asterias rubens from these ...200312959528
[effects of excision of the ocular peduncles on the development of the male genital apparatus of carcinus maenas pennant]. 195313059934
[the existence of two colonies of carcinus maenas pennant and the morphological characteristics which differentiate them]. 195313059946
[the nature of the carotenoid pigments of carcinus maenas pennant]. 195313059947
[biologic comparison of two populations of carcinus maenas pennant: moult of puberty]. 195313059965
[localization and metabolism of carotenoid pigments in the crab carcinus maenas]. 195313067246
[lipid content and determinism of external sex characteristics in the crab carcinus maenas pennant, parasited by rhizocephalus sacculina carcini thompson]. 195313067320
[absorption of carotenoid pigments from carcinus maenas pennat by its parasite sacculina carcini thompson]. 195413150622
[effect of ablation of ocular peduncles on the frequency of oviposition in carcinus maenas pennant]. 195413161398
[new experiments on movements of optic peduncles in carcinus maenas]. 195413200490
an aminosugar nucleotide from carcinus maenas. 195813560431
[nature of the protein fractions isolated from the serum of carcinus maenas leach by zone electrophoresis]. 195813619110
[starch gel electrophoresis of the blood proteins of carcinus maenas l]. 195813619137
[ocular peduncles, molting gland and genital apparatus in carcinus maenas l]. 195913663291
observations on the localisation and chemical constitution of neurosecretory material in nerve terminals in carcinus maenas. 195913669949
[amino acid constituents of tissues in crustacea. i. amino acid constituents in muscles of carcinus maenas l. in passage from sea water to brine & during molting]. 195913670672
a chitinase system from carcinus maenas. 196013764353
the fine structure of respiring particles isolated from the hepatopancreas of the crab carcinus maenas. 196213866015
respiratory chain phosphorylation in subcellular particles isolated from the hepatopancreas of carcinus maenas. 196113866505
some spectrophotometric studies on respiring particles isolated from the hepatopancreas of carcinus maenas. 196113866506
peripheral reflex inhibitionin the claw of the crab, carcinus maenas (l.). 196213875124
proprioceptive reflexes in the legs of carcinus maenas (l.). 196213875125
the preparation and properties of sub-cellular respiring particles (mitochonria) from the hepatopancreas of carcinus maenas. 196213936596
the effect of osmotic pressure on the activity of carcinus maenas mitochondria. 196213936597
[conditioned food reflexes from the visual, tactile and static receptors of the black sea crab carcinus maenas]. 200813962447
[on a l-serine dehydrase of the hepatopancreas of carcinus maenas l]. 196113966662
mitochondria from the crab, carcinus maenas 196314010258
[on the use of single nerve fibers of the crab carcinus maenas for the study of biopotentials]. 201514183882
secondary immune response to bacteriophage t1 in the shore crab, carcinus maenas. 196414207295
the spectrophotometric dissection of the electron transfer system of mitochondria isolated from the hepatopancreas of carcinus maenas. 196414210628
the identification of an alpha-amylase in aqueous extracts of the hepatopancreas of carcinus maenas, the common shore crab. 196414221833
distribution of phthalocyanine dye (heliogen blue sbl) in ultrastructures of vital dye axons of the crab carcinus maenas. 196414222284
[the effect of potassium, rubidium and cesium ions on the resting potential of muscle fibers of the crab carcinus maenas]. 201514244498
[on the life cycle of a diphyllidean cestode. identification of the larva in carcinus maenas (l. 1758), intermediary host]. 200714246130
proprioception by chordotonal organs in the mero-carpopodite and carpo-propodite joints of carcinus maenas legs. 196514288198
proprioception by the coxo-basal chordotonal organ, cb, in legs of the crab, carcinus maenas. 196514323766
[gonad implants in the crab carcinus maenas pennant]. 195514364897
histamine content of tissues from the crab carcinus maenas. 196114455267
the larval chromatophoral system of the crab, carcinus maenas (l). 196114484566
salinity-mediated carbonic anhydrase induction in the gills of the euryhaline green crab, carcinus maenas.the euryhaline green crab, carcinus maenas, is a relatively strong osmotic and ionic regulator, being able to maintain its hemolymph osmolality as much as 300 mosm higher than that in the medium when the crab is acclimated to low salinity. it makes the transition from osmoconformity to osmoregulation at a critical salinity of 26 ppt, and new acclimated concentrations of hemolymph osmotic and ionic constituents are reached within 12 h after transfer to low salinity. one of the central features of ...200314511744
cyp330a1 and cyp4c39 enzymes in the shore crab carcinus maenas: sequence and expression regulation by ecdysteroids and xenobiotics.cytochrome p450 enzymes (cyp enzymes) catalyse important metabolic reactions of exogenous and endogenous substrates, including steroid hormones. here, we report the first two cyp sequences from the shore crab, carcinus maenas. two complete cdnas isolated from crab hepatopancreas encode cyp enzymes named cyp330a1, the first member of a new family, and cyp4c39. cyp330a1 is closest related to members of the cyp2 family (37.3% identical to mouse cyp2j6) and cyp4c39 is most identical to crayfish cyp4 ...200314521903
effect of low salinity on cadmium accumulation and calcium homeostasis in the shore crab (carcinus maenas) at fixed free cd2+ concentrations.increased cd toxicity at low salinity has been attributed to increased free cd2+ ion concentration ([cd2+]sw), but transfer to dilute seawater also stimulates physiological ionic regulation in crabs. in this study, cd accumulation and ca homeostasis in the shore crab (carcinus maenas) were explored at fixed [cd2+]sw to reveal the physiological events during sublethal cd exposure. crabs were exposed to 3.4 or 34 microg/l [cd2+], in both 100% seawater (sw) and 33% sw for up to 10 d and sampled for ...200314587919
ontogeny of osmoregulatory structures and functions in the green crab carcinus maenas (crustacea, decapoda).the ontogeny of osmoregulation, the development of branchial transporting epithelia and the expression of the enzyme na+/k+-atpase were studied in carcinus maenas (l.) obtained from the north sea, germany. laboratory-reared zoea larvae, megalopae and young crabs were exposed to a wide range of salinities, and hemolymph osmolality was measured after 24 h exposure time (72 h in juveniles). zoea i larvae slightly hyper-regulated in dilute media (10.2 per thousand and 17.0 per thousand ) and osmocon ...200414668316
response in nematocyst uptake by the nudibranch flabellina verrucosa to the presence of various predators in the southern gulf of maine.aeolid nudibranchs maintain nematocysts sequestered from their cnidarian prey for protection against predators. selection for nematocyst incorporation is a function of diet and prey choice, but ratios vary among nudibranchs feeding on a given diet, indicating that other factors may be involved. it is proposed that the presence of predators influences nematocyst incorporation. nematocyst uptake in the nudibranch flabellina verrucosa collected from the southern gulf of maine was examined in respon ...200314672990
acetylcholine formation in the central nervous system of carcinus maenas. 195014770883
crustins, homologues of an 11.5-kda antibacterial peptide, from two species of penaeid shrimp, litopenaeus vannamei and litopenaeus setiferus.the response of crustaceans to pathogens is believed to depend solely on innate, nonadaptive immune mechanisms, including phagocytosis, encapsulation, clotting, and a variety of soluble antimicrobial activities. arthropod antimicrobial peptides, while characterized primarily from insects, also have been isolated from crustaceans. expressed sequence tag analysis of hemocyte complementary dna libraries from 2 species of shrimp, litopenaeus vannamei and litopenaeus setiferus, revealed transcripts w ...200214961261
rapid assessment of polycyclic aromatic hydrocarbon (pah) exposure in decapod crustaceans by fluorimetric analysis of urine and haemolymph.polycyclic aromatic hydrocarbons (pahs) are ubiquitous and potentially harmful contaminants of the coastal and marine environment. studies of their bioavailability, disposition and metabolism in marine organisms are therefore important for environmental monitoring purposes. detecting pah compounds in the biological fluids of marine organisms provides a measure of their environmental exposure to pahs. in the present study, the shore crab carcinus maenas was exposed to waterborne pyrene for 48h. u ...200415003698
histopathological effects of trypanorhynch metacestodes in the digestive gland of a novel host, carcinus maenas (decapoda).the green crab carcinus maenas was introduced to australian temperate waters in the late 1800s, has since become established, and is now considered to be a pest. we undertook an extensive parasite survey to find potential natural enemies of c. maenas and found it to be infected in australia by 2 species of larval trypanorhynch tapeworm, trimacracanthus aetobatidis and dollfusiella martini. we describe the gross pathology and histopathology of the parasites' new host (c. maenas) and note that the ...200415038453
a multibiomarker approach to environmental assessment.incorporation of ecologically relevant biomarkers into routine environmental management programs has been advocated as a pragmatic means of linking environmental degradation with its causes. here, suites of biomarkers, devised to measure molecular damage, developmental abnormality and physiological impairment, were combined with chemical analysis to determine exposure to and the effects of pollution at sites within southampton water (uk). test species included a filter feeder, a grazer, and an o ...200415074681
differential sensitivity of three marine invertebrates to copper assessed using multiple biomarkers.understanding how biomarkers relate to each other on exposure to particular contaminants in different species is key to their widespread application in environmental management. however, few studies have systematically used multiple biomarkers in more than a single species to determine the variability of sublethal effects of a particular contaminant. in this study, three marine invertebrates, the shore crab carcinus maenas, the common limpet patella vulgata and the blue mussel mytilus edulis, we ...200415129769
evaluation of fixed wavelength fluorescence and synchronous fluorescence spectrophotometry as a biomonitoring tool of environmental contamination.a field study was carried out to investigate whether polyaromatic hydrocarbon (pah) contamination in shore crabs (carcinus maenas) could be measured using fluorescence spectrometry. crabs were collected as part of an integrated study to assess pah contamination in southampton water, uk. urine was collected from crabs via a non-destructive technique and analysed using fixed wavelength fluorescence (ff) and synchronous fluorescence spectrophotometry (sfs) in order to determine the presence of pah ...201615178045
temperature-dependent physiological response of carcinus maenas exposed to copper.the effect of temperature on the heart rate (fh) of carcinus maenas exposed to a sublethal nominal concentration (0.5 mg l(-1)) of copper is reported. adult, intermoult males (4 cm carapace width) were collected from stavanger fjord (norway) in august (seawater temperature=17 degrees c) and maintained in the laboratory (fed ad libitum) at 5, 15 and 25 degrees c for 7 days. following this holding period, crabs were exposed to waterborne copper at the same temperature. after 3 days of exposure, in ...200415178113
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