Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| generation, annotation and analysis of ests from trichoderma harzianum cect 2413. | the filamentous fungus trichoderma harzianum is used as biological control agent of several plant-pathogenic fungi. in order to study the genome of this fungus, a functional genomics project called "trichoest" was developed to give insights into genes involved in biological control activities using an approach based on the generation of expressed sequence tags (ests). | 2006 | 16872539 |
| biochemical characterization of an extracellular polygalacturonase from trichoderma harzianum. | an extracellular polygalacturonase (pgii) from trichoderma harzianum was purified to homogeneity by two chromatography steps using deae-sepharose and sephacryl s-200. the molecular weight of t. harzianum pgii was 31,000 da by gel filtration and sds-page. pgii had isoelectric point of 4.5 and optimum ph of 5.0. pgii was very stable at the ph 5.0. the extent of hydrolysis of different pectins by enzyme was decreased with increasing of degree of esterification (de). pgii had very low activity towar ... | 2006 | 16872705 |
| transgenic tobacco plants overexpressing chitinases of fungal origin show enhanced resistance to biotic and abiotic stress agents. | genes encoding defense-related proteins have been used to alter the resistance of plants to pathogens and other environmental challenges, but no single fungal gene overexpression has produced broad-spectrum stress resistance in transgenic lines. we have generated transgenic tobacco (nicotiana tabacum) lines that overexpress the endochitinases chit33 and chit42 from the mycoparasitic fungus trichoderma harzianum and have evaluated their tolerance to biotic and abiotic stress. both chit33 and chit ... | 2006 | 16891545 |
| susceptibility of the ant-cultivated fungus leucoagaricus gongylophorus (agaricales: basidiomycota) towards microfungi. | the aim of this study was to select virulent strains of microfungi against leucoagaricus gongylophorus, a symbiotic fungus cultivated by leaf-cutting ants. the results from in vitro assays showed that microfungal strains had a variable and significant impact on the colony development of l. gongylophorus. specifically, trichoderma harzianum, escovopsis weberi cbs 810.71 and e. weberi a088 were more effective, inhibiting the l. gongylophorus colonies by 75, 68 and 67%, respectively (p < 0.05) afte ... | 2006 | 16897590 |
| xyloglucanases in the interaction between saprobe fungi and the arbuscular mycorrhizal fungus glomus mosseae. | we studied the production of xyloglucanase enzymes of pea and lettuce roots in the presence of saprobe and arbuscular mycorrhizal (am) fungi. the am fungus glomus mosseae and the saprobe fungi fusarium graminearum, fusarium oxysporum-126, trichoderma harzianum, penicillium chrysogenum, pleurotus ostreatus and aspergillus niger were used. g. mosseae increased the shoot and root dry weight of pea but not of lettuce. most of the saprobe fungi increased the level of mycorrhization of pea and lettuce ... | 2007 | 16904235 |
| the chemistry of the bio-control agent, trichoderma harzianum. | the role of 6-n-pentyl-2h-pyran-2-one and other metabolites of the fungus trichoderma harzianum, in its application as a bio-control agent, is discussed. | 2005 | 16961094 |
| in vitro activity and synergism of amphotericin b, azoles and cationic antimicrobials against the emerging pathogen trichoderma spp. | the uncommon fungal pathogen trichoderma shows increasing medical importance particularly in immunocompromised patients. despite systemic antifungal therapy, prognosis of trichoderma infection is poor regardless of the type of infection and the therapy used. the aim of the present study was to evaluate the in vitro activity and synergism of double antifungal combinations including amphotericin b, voriconazole, fluconazole, chlorhexidine digluconate and akacid plus against 15 isolates of trichode ... | 2006 | 16987867 |
| studies on the interaction between grape-associated filamentous fungi on a synthetic medium. | eleven fungi isolated from grapes and sun-dried grapes (alternaria alternata, cladosporium herbarum, eurotium amstelodami, penicillium janthinellum, p. decumbens, trichoderma harzianum, candida sp., aspergillus carbonarius ota-negative, a. carbonarius ota-positive, a. niger var. niger. and a. japonicus var. aculeatus), were grown in snm medium at different water activities (0.82-0.97) and temperature (20-40 degrees c) levels for 18 days. pairs of one aspergillus faced with one non-aspergillus we ... | 2007 | 16996158 |
| heterologous expression and site-directed mutagenesis studies of two trichoderma harzianum chitinases, chit33 and chit42, in escherichia coli. | heterologous expression of two fungal chitinases, chit33 and chit42, from trichoderma harzianum was tested in the different compartments and on the surface of escherichia coli cells. our goal was to find a fast and efficient expression system for protein engineering and directed evolution studies of the two fungal enzymes. cytoplasmic overexpression resulted in both cases in inclusion body formation, where active enzyme could be recovered after refolding. periplasmic expression of chit33, and es ... | 2007 | 16997574 |
| inhibition of aflatoxin b production of aspergillus flavus, isolated from soybean seeds by certain natural plant products. | the inhibitory effect of cowdung fumes, captan, leaf powder of withania somnifera, hyptis suaveolens, eucalyptus citriodora, peel powder of citrus sinensis, citrus medica and punica granatum, neem cake and pongamia cake and spore suspension of trichoderma harzianum and aspergillus niger on aflatoxin b(1) production by toxigenic strain of aspergillus flavus isolated from soybean seeds was investigated. | 2006 | 17032218 |
| leaf hairs influence phytopathogenic fungus infection and confer an increased resistance when expressing a trichoderma alpha-1,3-glucanase. | the leaf surface of a very large number of plant species are covered by trichomes. non-glandular trichomes are specialized unicellular or multicellular structures that occur in many different plant species and function in xenobiotic detoxification and protecting the plant against pest attack. by analysing the susceptibility of trichome mutants, evidence is provided that indicates the influence of leaf trichomes on foliar fungal infections in arabidopsis thaliana, probably by facilitating the adh ... | 2006 | 17043085 |
| exploitation of trichoderma harzianum mycelial waste for the removal of rhodamine 6g from aqueous solution. | the harvested mycelial waste of trichoderma harzianum was used as an adsorbent for the removal of rhodamine 6g and was studied in batch mode. the effects of agitation time and initial dye concentration, adsorbent dosage and ph were examined. the study revealed that the amount of dye adsorbed (mgg(-1)) increased with increase in agitation time and reached equilibrium after 120 min, for dye concentrations of 10-50 mg l(-1). the adsorbent dosage of 1.0 g/50 ml and ph of 8.0 were found to be optimum ... | 2007 | 17045731 |
| biological control of peach fungal pathogens by commercial products and indigenous yeasts. | the potential use of the commercial biocontrol products serenade (bacillus subtilis qst-713) and trichodex (trichoderma harzianum rifai strain t39) to inhibit the postharvest pathogenic molds penicillium crustosum and mucor circinelloides was investigated. both products exhibited antagonistic activity in vitro against the pathogens, reducing their growth at different levels. in addition, epiphytic yeasts isolated from peaches were identified as candida maltosa, pichia fermentans, and pichia kluy ... | 2006 | 17066929 |
| efficient isolation of anthraquinone-derivatives from trichoderma harzianum ets 323. | anthraquinone-derivatives, chrysophanol and pachybasin, were purified by a silica column chromatography with two different solvent systems from trichoderma harzianum ets 323. the fungus was incubated in sugarcane bagasse solid medium at room temperature without rotation. structure of chrysophanol was solved by x-ray diffraction and pachybasin by nmr spectra. about 233+/-13 mg of pure chrysophanol and 773+/-40 mg of pure pachybasin were recovered per kg of solid cultural medium, with yields 1.7+/ ... | 2007 | 17067682 |
| fungal biosynthesis of endochitinase and chitobiase in solid state fermentation and their application for the production of n-acetyl-d-glucosamine from colloidal chitin. | the present study was directed to the production of n-acetyl-d-glucosamine using endochitinase and chitobiase from fungal cultures in solid culturing. fifteen fungal strains were evaluated for endochitinase and chitobiase production under solid-state fermentation using agro-industrial residues, of which penicillium aculeatum nrrl 2129 showed maximum endochitinase activity whereas trichoderma harzianum tubf 927 showed maximum chitobiase activity. eleven substrates, alone and in combination with c ... | 2007 | 17092709 |
| hybrid mitochondrial plasmids from senescence suppressor isolates of neurospora intermedia. | we analyzed several natural suppressor isolates of the pkalilo-based fungal senescence syndrome of neurospora intermedia. the pkalilo plasmid did not increase in titer in these isolates. nor did it show integration "de novo." in at least two of the senescence suppressor isolates, pkalilo had formed stable recombinants with other mitochondrial elements. pkalilo/mtdna recombination junctions were complete and appeared to have been formed via a nonhomologous recombination mechanism. further analysi ... | 2007 | 17151255 |
| [extracellular proteinases of filamentous fungi as potential markers of phytopathogenesis]. | the presence of proteins in the culture liquid of filamentous fungi under study was found to induce the secretion of proteinases. the inhibitory analysis of the major extracellular proteinases of the saprotrophic fungus trichoderma harzianum and the phytopathogenic fungus alternaria alternata showed that they both belong to the group of serine proteinases. the substrate specificity of these proteinases and their sensitivity to inhibitors suggest that the enzyme of t. harzianum is a subtilisin-li ... | 2006 | 17205798 |
| partial silencing of a hydroxy-methylglutaryl-coa reductase-encoding gene in trichoderma harzianum cect 2413 results in a lower level of resistance to lovastatin and lower antifungal activity. | in the present article, we describe the cloning and characterization of the trichoderma harzianum hmgr gene encoding a hydroxymethylglutaryl coa reductase (hmgr), a key enzyme in the biosynthesis of terpene compounds. in t. harzianum, partial silencing of the hmgr gene gave rise to transformants with a higher level of sensitivity to lovastatin, a competitive inhibitor of the hmgr enzyme. in addition, these hmgr-silenced transformants produced lower levels of ergosterol than the wild-type strain ... | 2007 | 17218128 |
| a fermentation system designed to independently evaluate mixing and/or oxygen tension effects in microbial processes: development, application and performance. | in order to evaluate the independent effects of hydrodynamic conditions and/or oxygen tension on culture physiology and productivity, a fermentation system designed to control dissolved oxygen at constant power drawn (p/v) was developed. the system included a fully instrumented 14 l bioreactor coupled to a pc for data acquisition and control. power drawn was measured (using a commercial torquemeter coupled to the shaft) and maintained constant by varying the agitation speed; while gas blending w ... | 2007 | 17225992 |
| in vitro suppression of fungi caused by combinations of apparently non-antagonistic soil bacteria. | we hypothesized that apparently non-antagonistic soil bacteria may contribute to suppression of fungi during competitive interactions with other bacteria. four soil bacteria (brevundimonas sp., luteibacter sp., pedobacter sp. and pseudomonas sp.) that exhibited little or no visible antifungal activity on different agar media were prescribed. single and mixed strains of these species were tested for antagonism on a nutrient-poor agar medium against the plant pathogenic fungi fusarium culmorum and ... | 2007 | 17233750 |
| removal and recovery of uranium from aqueous solutions by trichoderma harzianum. | removal and recovery of uranium from dilute aqueous solutions by indigenously isolated viable and non-viable fungus (trichoderma harzianum) and algae (rd256, rd257) was studied by performing biosorption-desorption tests. fungal strain was found comparatively better candidate for uranium biosorption than algae. the process was highly ph dependent. at optimized experimental parameters, the maximum uranium biosorption capacity of t. harzianum was 612 mg u g(-1) whereas maximum values of uranium bio ... | 2007 | 17291559 |
| fermentation and antimicrobial activities of extracts from different species of fungus belonging to genus, trichoderma. | the present paper discusses the effects of the crude extracts of trichoderma spp. on a number of fungal and bacterial organisms. these include paecilomyces variotii, penicillium notatum, nematospora corylii, mucor miehei, bacillus brevis, bacillus subtilis, enterobacter dissolvens and sarcina lutea. the culture broth extracts of different isolates of trichoderma harzianum, t. longibrachiatum and t. koningii cultured in different media were investigated individually for in-vitro antifungal and an ... | 2004 | 17298115 |
| qid74 cell wall protein of trichoderma harzianum is involved in cell protection and adherence to hydrophobic surfaces. | trichoderma is widely used as biocontrol agent against phytopathogenic fungi, and as biofertilizer because of its ability to establish mycorriza-like association with plants. the key factor to the ecological success of this genus is the combination of very active mycoparasitic mechanisms plus effective defense strategies induced in plants. this work, different from most of the studies carried out that address the attacking mechanisms, focuses on elucidating how trichoderma is able to tolerate ho ... | 2007 | 17300969 |
| influence of autoclaved saprotrophic fungal mycelia on proteolytic activity in ectomycorrhizal fungi. | the production of proteolytic enzymes by several strains of ectomycorrhizal fungi i.e., amanita muscaria (16-3), laccaria laccata (9-12), l. laccata (9-1), suillus bovinus (15-4), suillus bovinus (15-3), suillus luteus (14-7) on mycelia of trichoderma harzianum, trichoderma virens and mucor hiemalis and sodium caseinate, yeast extract was evaluated. the strains of a. muscaria (16-3) and l. laccata (9-12) were characterized by the highest activity of the acidic and neutral proteases. taking the m ... | 2007 | 17333458 |
| trichoderma harzianum rifai 1295-22 mediates growth promotion of crack willow (salix fragilis) saplings in both clean and metal-contaminated soil. | we investigated if the plant growth promoting fungus trichoderma harzianum rifai 1295-22 (also known as "t22") could be used to enhance the establishment and growth of crack willow (salix fragilis) in a soil containing no organic or metal pollutants and in a metal-contaminated soil by comparing this fungus with noninoculated controls and an ectomycorrhizal formulation commercially used to enhance the establishment of tree saplings. crack willow saplings were grown in a temperature-controlled gro ... | 2007 | 17345130 |
| in vitro biocontrol analysis of alternaria alternata (fr.) keissler under different environmental conditions. | the species trichoderma harzianum was analyzed as possible biocontrol agent of alternaria alternata under different environmental conditions (water activity and temperature). the strains were analyzed macroscopically to obtain the index of dominance. the analysis was completed using two microscopic techniques. t. harzianum showed dominance on contact over a. alternata at all testing temperatures and water activities tested except at 0.95 a(w) and 15 degrees c, at which t. harzianum inhibited a. ... | 2007 | 17356789 |
| biological control of botrytis gray mould on tomato cultivated in greenhouse. | research was carried out to evaluate the effectiveness of the biological control of the botrytis gray mould, caused by botrytis cinerea pers., one of the most important fungal diseases of the tomato (lycopersicon esculentum mill.). biological control was performed by using trichoderma harzianum rifai, an antagonist that is a naturally occurring fungus found on some plants and in the soil worldwide. trichoderma spp. are fungi diffused in nearly all agricultural soils and in other environments suc ... | 2006 | 17390837 |
| use of trichoderma harzianum and trichoderma viride for the biological control of meloidogyne incognita on tomato. | trichoderma harzianum and trichoderma viride were tested for their capacity to reduce the incidence of the root-knot nematode meloidogyne incognita on tomato. in vitro studies demonstrated that all tested isolates were effective in causing nematode mortality compared with the control. trichoderma slightly reduced nematode damage to tomato in vivo. treatment of the soil with the biocontrol agents before transplanting, improved control over treatment directly at transplanting. the trichoderma isol ... | 2006 | 17390844 |
| biochemical characterization of biocontrol agents used for control of root pathogens. | due to low microbial biodiversity in the nutrient solution and the root, open and closed hydroponic systems are regarded as sensitive from biological point of view. this is of particular importance as an enhanced spread of root pathogen may occur in closed hydroponic systems and possibilities for chemical control are restricted. biocontrol agents appear to be a feasible alternative. for an effective use of these agents, knowledge on the behaviour, external requirements and the specific capabilit ... | 2006 | 17390847 |
| screening of bioagents against root rot of mung bean caused by rhizoctonia solani. | a laboratory and green house experiment was carried out on the comparative antagonistic performance of four different bioagents (aspergillus sp. gliocladium virens, trichoderma harzianum and t. viride) isolated from soil against rhizoctonia solani. under laboratory conditions, t. harzianum exhibited maximum (75.55%) mycelial growth inhibition of r. solani. this was followed by t. viride, which showed 65.93% mycelial growth inhibition of the pathogen. gliocladium virens was also found to be effec ... | 2006 | 17390853 |
| microscopic and transcriptome analyses of early colonization of tomato roots by trichoderma harzianum. | the capacity of the fungus trichoderma harzianum cect 2413 to colonize roots and stimulate plant growth was analyzed. tobacco seedlings (nicotiana benthamiana) transferred to petri dishes inoculated with t. harzianum conidia showed increased plant fresh weight (140%) and foliar area (300%), as well as the proliferation of secondary roots (300%) and true leaves (140%). the interaction between strain cect 2413 and the tomato-root system was also studied during the early stages of root colonization ... | 2007 | 17407057 |
| [reaction of microorganisms to the digestive fluid of the earthworms]. | the reaction of soil bacteria and fungi to the digestive fluid of the earthworm aporrectodea caliginosa was studied. the fluid was obtained by centrifugation of the native enzymes of the digestive tract. the inhibition of growth of certain bacteria, spores, and fungal hyphae under the effect of extracts from the anterior and middle sections of the digestive tract of a. caliginosa was discovered for the first time. in bacteria, microcolony formation was inhibited as early as 20-30 s after the app ... | 2007 | 17410875 |
| characterization of genes encoding novel peptidases in the biocontrol fungus trichoderma harzianum cect 2413 using the trichoest functional genomics approach. | proteolytic enzymes (ec 3.4) secreted by trichoderma strains are receiving increasing attention because of their potential implication in the trichoderma biocontrol abilities. we have used an expressed sequence tag (est) approach to identify genes encoding extracellular peptidases in t. harzianum cect 2413 grown under several biocontrol-related conditions. based on blastx results and gene ontology annotation, a total of 61 (among 3478) unique sequences (unisequences) were predicted to encode enz ... | 2007 | 17415567 |
| colony morphology mutants of trichoderma harzianum with increased beta-1,4-n-acetyl-glucosaminidase production. | a total of 36 uv-induced mutants with altered colony morphology were isolated from strain trichoderma harzianum t334, a potential biocontrol agent against plant pathogenic fungi with the ability to produce constitutively low levels of chitinases. the level of constitutive beta-1,4-n-acetyl-glucosaminidase production in standing and shaken cultures under non-inductive conditions was tested in mutants and compared to that of the parental strain. about 30% of the mutants showed significantly increa ... | 2007 | 17523389 |
| mycoparasitism studies of trichoderma harzianum strains against rhizoctonia solani: evaluation of coiling and hydrolytic enzyme production. | the genus trichoderma is a potential biocontrol agent against several phytopathogenic fungi. one parameter for its successful use is an efficient coiling process followed by a substantial production of hydrolytic enzymes. the interaction between fifteen isolates of trichoderma harzianum and the soil-borne plant pathogen, rhizoctonia solani, was studied by light microscopy and transmission electron microscopy (tem). macroscopic observations of fungal growth in dual cultures revealed that growth i ... | 2007 | 17534583 |
| isolation and characterization of a beta-tubulin gene from trichoderma harzianum. | 2007 | 17551827 | |
| inhibition of inducible tumor necrosis factor-alpha expression by the fungal epipolythiodiketopiperazine gliovirin. | tnf-alpha is a major pro-inflammatory cytokine that regulates further cytokine induction, especially of il-1 and il-6, in many human diseases including cancer, inflammation and immune disorders. in a search for new inhibitors of inducible tnf-alpha promoter activity and expression, cultures of the imperfect fungus trichoderma harzianum were found to produce gliovirin, a previously isolated epipolythiodiketopiperazine. gliovirin inhibited inducible tnf-alpha promoter activity and synthesis in lps ... | 2007 | 17552910 |
| the influence of circulation frequency on fungal morphology: a case study considering kolmogorov microscale in constant specific energy dissipation rate cultures of trichoderma harzianum. | the energy dissipation/circulation function (edcf) is the product of the specific energy dissipation rate in the impeller swept volume (p/kd(3)) and the frequency of particle circulation (1/t(c)) through that volume. a direct relationship between mycelial fragmentation and edcf has been reported. however, and although hyphal fragmentation is assumed to occur by hyphae-eddy interaction, kolmogorov microscale (lambda) has not been shown to determine, at least directly, fungal morphology. in this w ... | 2007 | 17586080 |
| trichoderma harzianum: a biocontrol agent against bipolaris oryzae. | rice brown spot, caused by bipolaris oryzae, can be a serious disease causing a considerable yield loss. trichoderma harzianum is an effective biocontrol agent for a number of plant fungal diseases. thus, this research was carried out to investigate the mechanisms of action by which t. harzianum antagonizes bipolaris oryzae in vitro, and the efficacy of spray application of a spore suspension of t. harzianum for control of rice brown spot disease under field conditions. in vitro, the antagonisti ... | 2007 | 17592758 |
| screening of bioagents against root rot of mung bean caused by rhizoctonia solani. | a laboratory and green house experiment was carried out on the comparative antagonistic performance of four different bioagents (aspergillus sp., gliocladium virens, trichoderma harzianum and t. viride) isolated from soil against rhizoctonia solani. under laboratory conditions, t. harzianum exhibited maximum (75.55%) mycelial growth inhibition of r. solani this was followed by t. viride, which showed 65.93 per cent mycelial growth inhibition of the pathogen. gliocladium virens was also found to ... | 2006 | 17612349 |
| microbial toxicity of pesticide derivatives produced with uv-photodegradation. | our study aimed at acquiring information about the biological effect of pesticides and their degradates produced by uv-treatment on microbiological activity. five photosensitive pesticides (carbendazim, acetochlor, simazine, chlorpyrifos, eptc) and six representative soil microbes (bacillus subtilis, pseudomonas fluorescens, mycobacterium phlei, fusarium oxysporum, penicillium expansum, trichoderma harzianum) were applied throughout our model experiments. the antimicrobial effects of the pestici ... | 2007 | 17639315 |
| human exposure to airborne fungi from genera used as biocontrol agents in plant production. | the fungi trichoderma harzianum, t. polysporum, t. viride, paeciliomyces fumosoroseus, p. lilacinus, verticillium/lecanicillium lecanii, ulocladium oudemansii, u. atrum and beauveria bassiana are used or considered to be used for biocontrol of pests and plant diseases. human exposure to these fungi in environments where they may naturally occur or are used as biocontrol agents has not been directly investigated to date. this review aims to provide an overview of the current knowledge of human ex ... | 2007 | 17655172 |
| [mycoparasites effect on reproductive ability of sclerotinia sclerotiorum sclerotia.]. | the ability to parasitise sclerotinia sclerotiorum and the effect on apothecia production was evaluated for the following antagonists: trichoderma harzianum; trichoderma koningii; gliocladium roseum and chaetomium globosum. plastic trays were filled with of steam-sterilized soil. each one of them was infested with sclerotia of s. sclerotiorum and the culture of the antagonists. the trays were kept in a greenhouse and after 30, 60 and 90 days, evaluations were made. the rates of carpogenic germin ... | 1998 | 17655415 |
| chitosan-mediated changes in cell wall composition, morphology and ultrastructure in two wood-inhabiting fungi. | the effect of chitosan on cell wall deposition was investigated in the two wood-inhabiting fungal species trichoderma harzianum (cbs 597.91) and sphaeropsis sapinea (nzfs 2725). the study used three independent analytical techniques to quantify chitin in the fungal mycelium. a colorimetric method for the detection of d-glucosamine was compared with two gas chromatography-mass spectroscopy (gc-ms) methods employing alditol acetates analysis and pyrolysis. the latter used a stable-isotope-labelled ... | 2007 | 17707625 |
| application of dna bar codes for screening of industrially important fungi: the haplotype of trichoderma harzianum sensu stricto indicates superior chitinase formation. | selection of suitable strains for biotechnological purposes is frequently a random process supported by high-throughput methods. using chitinase production by hypocrea lixii/trichoderma harzianum as a model, we tested whether fungal strains with superior enzyme formation may be diagnosed by dna bar codes. we analyzed sequences of two phylogenetic marker loci, internal transcribed spacer 1 (its1) and its2 of the rrna-encoding gene cluster and the large intron of the elongation factor 1-alpha gene ... | 2007 | 17827332 |
| effect of diatomaceous earth and trichoderma harzianum t-22 (rifai strain krl-ag2) on the fungus gnat bradysia sp. nr. coprophila (diptera: sciaridae). | this study, consisting of three experiments, was designed to assess whether diatomaceous earth, when applied to the surface of growing media, reduces adult fungus gnat bradysia sp. nr. coprophila (diptera: sciaridae) emergence or inhibits the females from laying eggs; and whether fungus gnat adults are attracted to the fungus trichoderma harzianum t-22 (rifai strain krl-ag2) under laboratory conditions. in the first two experiments, diatomaceous earth was applied at two different thicknesses (3. ... | 2007 | 17849889 |
| ph and pac1 control development and antifungal activity in trichoderma harzianum. | in trichoderma harzianum cect 2413 external ph regulates essential functions such as growth rate, sporulation, cell and colony morphology, pattern of secreted proteins, gene expression or mycoparasitism-related enzymatic activities. ph regulation is mediated by the transcriptional factor pac1 (homologous to pacc regulator in other fungi), encoded by pac1 whose expression increases with ph. two pac1 mutants have been obtained from cect 2413: p2.32, that possesses an allele of pac1 active at any p ... | 2007 | 17881256 |
| cloning and characterization of bgn16.3, coding for a beta-1,6-glucanase expressed during trichoderma harzianum mycoparasitism. | to clone and characterize the gene coding for bgn16.3, a beta-1,6-glucanase putatively implicated in mycoparasitism by trichoderma harzianum, a biocontrol agent used against plant pathogenic fungi. | 2007 | 17897233 |
| evaluation of fungal culture filtrate containing chitinase as a biocontrol agent against helicoverpa armigera. | to evaluate the biocontrol efficacy of culture filtrate containing chitinase from trichoderma harzianum against heliothis. | 2007 | 17953595 |
| environmental dynamics of bacillus amyloliquefaciens ccmi 1051 antifungal activity under different nitrogen patterns. | the aim of this study was to evaluate the influence of environmental conditions on the antifungal activity of the bacillus sp. ccmi 1053 cultures. | 2008 | 17953685 |
| statistical optimization of process conditions for cellulase production by liquid state bioconversion of domestic wastewater sludge. | a two-level fractional factorial design (ffd) was used to determine the effects of six factors, i.e. substrate (domestic wastewater sludge - dws) and co-substrate concentration (wheat flour - wf), temperature, initial ph, inoculum size and agitation rate on the production of cellulase enzyme by trichoderma harzianum in liquid state bioconversion. on statistical analysis of the results from the experimental studies, optimum process conditions were found to be temperature 32.5 degrees c, substrate ... | 2008 | 17981027 |
| cloning and heterologous expression of aspartic protease sa76 related to biocontrol in trichoderma harzianum. | trichoderma harzianum is a soil-borne filamentous fungus that exhibits biological control properties because it parasitizes a large variety of phytopathogenic fungi. the production of hydrolytic enzymes appears to be a key element in the parasitic process. among the enzymes released by trichoderma, the aspartic proteases play a major role. a gene (sa76) encoding an aspartic protease was cloned by 3' rapid amplification of cdna ends from t. harzianum t88. the coding region of the gene is 1,593 bp ... | 2007 | 18031337 |
| the logarithmic transformation should be avoided for stabilising the variance of mould growth rate. | radial growth rate, micro (mm d(-1)) was evaluated by plotting the radius of the colony, r (mm) versus time (d) for alternaria alternata, aspergillus flavus, cladosporium cladosporioides, mucor racemosus, rhizopus oryzae and trichoderma harzianum at different t and a(w). for each of the 12 data sets, an analysis of variance of the raw growth rate data was performed. it was observed from the p-values that all square-root transformed values of micro were non-significant at the significance level a ... | 2008 | 18031858 |
| desorption of zinc by extracellularly produced metabolites of trichoderma harzianum, trichoderma reesei and coriolus versicolor. | to determine the role of fungal metabolites in the desorption of metals. | 2007 | 18045407 |
| effects of mutual interaction of laccaria laccata with trichoderma harzianum and t. virens on the morphology of microtubules and mitochondria. | organelles are known to respond to challenges caused by many stress factors. the morphology of the microtubular cytoskeleton and mitochondria during mutual interaction in coculture of laccaria laccata with trichoderma harzianum and t. virens were examined. hyphae from the interaction region were sampled between 4 and 12 days of growth. microtubules were labelled with a specific antibody and mitochondria with 3,3'-dihexyloxacarbocyanine iodide, and the organelles were examined microscopically. th ... | 2007 | 18176836 |
| water deficit as a driver of the mutualistic relationship between the fungus trichoderma harzianum and two wheat genotypes. | the aim of this study was to assess the occurrence of mutualistic interactions between the fungus trichoderma harzianum and two wheat genotypes, triticum aestivum cv. talhuén and t. turgidum subsp. durum cv. alifén, and the extent to which water deficit affected these interactions. two wheat genotypes were cultivated in the presence or absence of t. harzianum and in the presence or absence of water deficit. t. harzianum was in turn cultivated in the presence or absence of wheat plants and in the ... | 2008 | 18192424 |
| role of bacillus spp. in antagonism between pleurotus ostreatus and trichoderma harzianum in heat-treated wheat-straw substrates. | this study aimed to identify bacteria involved in trichodermaharzianum inhibition while promoting pleurotus ostreatus defences in order to favour cultivation-substrate selectivity for mushroom production. pcr-dgge profiles of total dna from wheat-straw substrate showed weak differences between bacterial communities from substrate inoculated with p. ostreatus with or without t. harzianum. the major cultivable bacteria were isolated from three batches of wheat-straw-based cultivation substrates sh ... | 2008 | 18295481 |
| defence-related gene expression in transgenic lemon plants producing an antimicrobial trichoderma harzianum endochitinase during fungal infection. | constitutive over-expression of antifungal genes from microorganisms involved in plant defence mechanisms represents a promising strategy for conferring genetic resistance against a broad range of plant pathogenic fungi. in the present work, two transgenic lemon clones with the chit42 gene from trichoderma harzianum were tested for resistance to fungal disease and expression level of defence-related genes was evaluated. different resistance-related processes, such as production of reactive oxyge ... | 2008 | 18306055 |
| management of root knot nematode on tomato through application of fungal antagonists, acremonium strictum and trichoderma harzianum. | management of root knot nematode disease infecting tomato, by the use of fungal bioagents acremonium strictum and trichoderma harzianum isolated from egg masses of m. incognita infecting tomato has been carried out. the rhizosphere and rhizoplane of root knot nematode infested tomato revealed consistent association of acremonium strictum. in the present study a. strictum and other fungal bioagents viz. aspergillus niger, paecilomyces lilacinus, rhizoctonia solani and trichoderma harzianum isolat ... | 2008 | 18368544 |
| [filamentous fungi preservation in distilled water.]. | some methods for keeping the fungal culture collection have been used. however, the choice of either one on the basis that must ensure the cultural genetic stability and its phenotipic characteristics. in this work the preservation method in distilled water recognized in the literature as a single, economic and certain method that guarantie the survival of fungus cultures for long periods was used. 26 strains of generus and species: aspergillus niger, aspergillus candidus, fusarium sp., fusarium ... | 1998 | 18473541 |
| cellulase production by trichoderma harzianum in static and mixed solid-state fermentation reactors under nonaseptic conditions. | cellulase production from lignocellulosic materials was studied in solid-state cultivation by both static and mixed techniques under nonaseptic conditions. the effects of fermentation conditions, such as moisture content, ph, temperature, and aeration, on cellulase production by trichoderma harzianum using a mixture of wheat straw (80%) and bran (20%) were investigated. with a moisture content of 74% and a ph of 5.8., 18 iu filter paper activity and 198 iu endoglucanase activity/g initial substr ... | 1985 | 18553830 |
| substrate conditions that influence the assays used for determining the beta-glucosidase activity of cellulolytic microorganisms. | culture filtrates from trichoderma harzianum e58, t. reesei cl 847 and penicillium sp. c 462 were assayed for beta-glucosidase activity using a range of substrates and sugar analysis methods. although sugar analyses by the dinitrosalicylic acid (dns) and nelson-somogyi methods gave a similar profile, when increasing concentrations of salicin were assayed, considerably higher values were obtained with the dns assay. the salicin concentration used for the assay greatly influenced the final beta-gl ... | 1986 | 18555279 |
| steam-explosion pretreatment of wood: effect of chip size, acid, moisture content and pressure drop. | material balances for pentosan, lignin, and hexosan, during steam-explosion pretreatment of aspenwood, showed almost quantitative recovery of cellulose in the water-insoluble fraction. dilute acid impregnation resulted in more selective hydrolysis of pentosan relative to undesirable pyrolysis, and gave a more accessible substrate for enzymatic hydrolysis. thermocouple probes, located inside simulated aspenwood chips heated in 240 degrees c-saturated steam, showed rapid heating of air-dry wood, w ... | 1986 | 18555395 |
| genome-wide identification, expression and chromosomal location of the genes encoding chitinolytic enzymes in zea mays. | chitinolytic enzymes are important pathogenesis and stress related proteins. we identified 27 putative genes encoding endochitinases in the maize genome via in silico techniques and four exochitinases. only seven of the endochitinases and segments of the exochitinases were heretofore known. the endochitinases included members of family 19 chitinases (classes i-iv of pr3, ii of pr4) and members of family 18 chitinases (class iii of pr8). some similar enzymes were detected on adjacent regions of t ... | 2008 | 18560892 |
| the molecular basis of shoot responses of maize seedlings to trichoderma harzianum t22 inoculation of the root: a proteomic approach. | trichoderma spp. are effective biocontrol agents for several soil-borne plant pathogens, and some are also known for their abilities to enhance systemic resistance to plant diseases and overall plant growth. root colonization with trichoderma harzianum rifai strain 22 (t22) induces large changes in the proteome of shoots of maize (zea mays) seedlings, even though t22 is present only on roots. we chose a proteomic approach to analyze those changes and identify pathways and genes that are involved ... | 2008 | 18562766 |
| inexpensive, rapid procedure for bulk purification of cellulase-free beta-1,4-d-xylanase of high specific activity. | a process has been developed for the bulk purification of cellulase-free beta-1,4-d-xylanase from the fungus trichoderma harzianum e58. the process involved the primary step of ultrafiltering the culture filtrate via a 10,000-molecular-weight cut-off membrane to separate the cellulase (retentate) and xylanase (permeate) fractions. the cellulase component was concentrated by 40- to 60-fold, resulting in an enzyme complex that could effectively hydrolyze high concentrations of cellulose and xylan ... | 1987 | 18576588 |
| recycle of enzymes and substrate following enzymatic hydrolysis of steam-pretreated aspenwood. | the commercial production of chemicals and fuels from lignocellulosic residues by enzymatic means still requires considerable research on both the technical and economic aspects. two technical problems that have been identified as requiring further research are the recycle of the enzymes used in hydrolysis and the reuse of the re calcitrant cellulose remaining after incomplete hydrolysis. enzyme recycle is required to lower the cost of the enzymes, while the reuse of the spent cellulose will low ... | 1987 | 18581434 |
| influence of growth substrate on production of cellulase enzymes by trichoderma harzianum e58. | cellulase production by trichoderma harzianum e58 grown on lactose and various cellulosic substrates such as solka floe, avicel, and steamed aspenwood was investigated. the culture filtrates of t. harzianum e58 obtained after growth on these substrates were assayed for their cellulase activities and overall hydrolytic activities. the severity of the steaming conditions used for the aspenwood had a pronounced effect on the cellulolytic activity of the produced culture filtrates. those substrates ... | 1988 | 18584671 |
| the interaction of xylanases with commercial pulps. | when purified xylanases from trichoderma harzianum e58 or from a clone of bacillus circulans were incubated with various low-yield wood pulps, little of the original enzyme activity could be detected in the filtrate at the end of the reaction. partial bleaching of the pulps prior to enzymatic treatment generally resulted in an increased recovery of the xylanase activity. it appears that both nonspecific adsorption and soluble inhibitors may be responsible for the loss of much of the xylanase act ... | 1991 | 18597365 |
| proteomic study of biocontrol mechanisms of trichoderma harzianum ets 323 in response to rhizoctonia solani. | to elucidate the entire range of proteins that are secreted by trichoderma harzianum ets 323 in its antagonism with rhizoctonia solani, an in vivo interaction between them was mimicked and not only the secreted cell wall-degrading enzymes (cwdes) but also all of the proteome were investigated. seven cwdes, chitinase, cellulase, xylanase, beta-1,3-glucanase, beta-1,6-glucanase, mannanase, and protease,were revealed by activity assay, in-gel activity stain, 2-de, and lc-ms/ms analysis. extracellul ... | 2008 | 18642836 |
| mutanase induction in trichoderma harzianum by cell wall of laetiporus sulphureus and its application for mutan removal from oral biofilms. | the cell wall material from fruiting bodies of laetiporus sulphureus has been suggested as a new alternative to mutan for the mutanase induction in trichoderma harzianum. structural analyses revealed that the alkali-soluble wall fraction from this polypore fungus contained 56.3% of (1-->3)-linked alpha-glucans. when the strain t. harzianum f-340 was grown on a cell wall preparation from l. sulphureus, the maximal enzyme productivity obtained after 3 days of cultivation was 0.71 u/ml. this yield ... | 2008 | 18667864 |
| induction of beta-1,3-glucanase in callus cultures in vitro. | sodium salicylate (nasa) increased induction of both intracellular and extracellular beta-1,3-glucanases in calluses of campion and duckweed. nasa concentrations from 30 to 100 mm were optimal for induction of intracellular glucanase in the campion callus, and for induction of extracellular glucanase the optimal concentration varied from 5 to 100 mm. the glucanase activity in the duckweed callus was lower than in the campion callus, and co-cultivation of the campion callus with trichoderma harzi ... | 2008 | 18707591 |
| characterization of a novel fungal chitosanase csn2 from gongronella sp. jg. | a 28kda chitosanase designated as csn2 was purified from the culture broth of the fungus gongronella sp. jg through three chromatography steps: cm-sepharose ff, superdex 200 and sp-sepharose ff. its optimal reaction ph and temperature were ph 5.6 and between 55 degrees c and 60 degrees c. the half-lives of csn2 at 50 degrees c and 55 degrees c were estimated to be 30min and 11min, respectively. the k(m) value of csn2 in sodium acetate buffer (ph 5.6) at 55 degrees c was 8.86mg/ml. mn(2+), ca(2+) ... | 2008 | 18722595 |
| bioactive lipopeptides of ice-nucleating snow bacterium pseudomonas syringae strain 31r1. | the production of secondary metabolite lipopeptides by ice-nucleating pseudomonas syringae strain 31r1 was investigated. pseudomonas syringae strain 31r1 is a rifampicin-resistant derivative of p. syringae no. 31 used for the commercial production of snow. it is shown that p. syringae strain 31r1 produces antifungal lipodepsipeptides, syringomycins e and g, and, in addition, a novel and unique lipopeptide, peptin31. spectroscopic and spectrometric analyses revealed that peptin31 is a linear unde ... | 2008 | 18789127 |
| overexpression of a trichoderma hsp70 gene increases fungal resistance to heat and other abiotic stresses. | all organisms share similar mechanisms in the heat shock response, such as synthesis of conserved heat shock proteins. here, we report on the cloning, characterization and functional analysis of a trichoderma harzianum t34 hsp70 gene. the expression of this gene was evaluated in cultures grown in abiotic stress conditions. an increased level of expression was detected when the fungus was grown at 37 or 41 degrees c, as well as in the presence of oxidative or osmotic agents. the overexpression of ... | 2008 | 18824239 |
| fungal diversity in the rhizosphere of endemic plant species of tenerife (canary islands): relationship to vegetation zones and environmental factors. | knowledge about fungal diversity scaling relationships relative to that of plants is important to understand ecosystem functioning. tenerife island, a natural laboratory to study terrestrial biodiversity, is represented by six different vegetation zones characterized by specific abiotic conditions and plant communities with a high proportion of endemic plants. little is known about the biodiversity of associated fungi. to understand the relationship between plant and fungal communities, we analy ... | 2009 | 18830279 |
| amendment with peony root bark improves the biocontrol efficacy of trichoderma harzianum against rhizoctonia solani. | we tested trichoderma harzianum as a biocontrol agent for rhizoctonia solani ag2-1, using six natural antifungal materials to improve its efficacy. among the six materials tested, peony (paeonia suffruticosa) root bark (prb) showed the strongest antifungal activity against r. solani ag2-1, and was not antagonistic to t. harzianum. scanning electron microscopy showed that treatment with prb extract resulted in shortened and deformed r. solani ag2-1 hyphal cells. the control of radish damping-off ... | 2008 | 18852509 |
| biological control of the root-knot nematode meloidogyne javanica by trichoderma harzianum. | abstract the fungal biocontrol agent, trichoderma harzianum, was evaluated for its potential to control the root-knot nematode meloidogyne javanica. in greenhouse experiments, root galling was reduced and top fresh weight increased in nematode-infected tomatoes following soil pretreatment with trichoderma peat-bran preparations. the use of a proteinase prb1-transformed line (p-2) that contains multiple copies of this gene improved biocontrol activity in the greenhouse experiments compared with t ... | 2001 | 18942999 |
| antagonism of nutrient-activated conidia of trichoderma harzianum (atroviride) p1 against botrytis cinerea. | abstract the effect of preliminary nutrient activation on the ability of conidia of the antagonist trichoderma harzianum (atroviride) p1 to suppress botrytis cinerea was investigated in laboratory, greenhouse, and field trials. preliminary nutrient activation at 21 degrees c accelerated subsequent germination of the antagonist at temperatures from 9 to 21 degrees c; at >/=18 degrees c, the germination time of preactivated t. harzianum p1 conidia did not differ significantly from that of b. ciner ... | 2001 | 18943332 |
| influence of a fungus-feeding nematode on growth and biocontrol efficacy of trichoderma harzianum. | abstract a fungivorous nematode, aphelenchoides sp., was isolated from field soil by baiting with mycelium of the biocontrol fungus trichoderma harzianum thzid1, and subsequently was maintained on agar cultures of the fungus. interactions between the nematode and the green fluorescent protein-producing transformant, t. harzianum thzid1-m3, were investigated in both heat-treated (80 degrees c, 30 min) and untreated field soil. thzid1-m3 was identified in soil by epifluorescence microscopy. when t ... | 2001 | 18943350 |
| individual-based approach to modeling hyphal growth of a biocontrol fungus in soil. | abstract fungi in soil perform beneficial roles that include biological control of soilborne plant pathogens. however, relatively little predictive information is available about the growth and activity of fungal hyphae in soil habitats. a stochastic computer simulation model ("fungmod") was developed to predict hyphal growth of the biocontrol fungus trichoderma harzianum thzid1 in soil. the model simulates a fungal colony as a population of spatially referenced hyphal segments, and is individua ... | 2006 | 18943499 |
| interactions between trichoderma harzianum strain t22 and maize inbred line mo17 and effects of these interactions on diseases caused by pythium ultimum and colletotrichum graminicola. | abstract seed treatment with trichoderma harzianum strain t22, which results in colonization of plant roots but little or no colonization of shoots or leaves, had substantial effects on growth of and disease expression in maize inbred line mo17. shoots and roots of 10-day-old seedlings grown in a sandy loam field soil were larger (roots were nearly twice as long) in the presence of t22 than in its absence. both main and secondary roots were increased in size and area and the root hair area was g ... | 2004 | 18943537 |
| use of green fluorescent protein and image analysis to quantify proliferation of trichoderma harzianum in nonsterile soil. | abstract one drawback of traditional methods for fungal biomass measurement is the inability to distinguish biomass of an introduced fungus from that of the indigenous microbial community in nonsterile soil. we quantified biomass of a specific fungal biological control agent in nonsterile soil using epifluorescence microscopy and image analysis of green fluorescent protein (gfp)-expressing trichoderma harzianum (thzid1-m3). numbers of colony forming units on a semiselective medium were compared ... | 2004 | 18943710 |
| disease progression by active mycelial growth and biocontrol of pythium ultimum var. ultimum studied using a rhizobox system. | abstract this study demonstrates that outward growth of mycelium from primary foci through bulk potting mix to roots of adjoining plants can be an important means of spread of damping-off and root rot caused by pythium ultimum. the use of a rhizobox system, which confines plant roots, enabled us to study the spread of actively growing mycelium between root systems placed at precise distances from each other. in steamed potting mix, hyphae of p. ultimum on average grew 9.6 cm from diseased root t ... | 2000 | 18944533 |
| expression of endochitinase from trichoderma harzianum in transgenic apple increases resistance to apple scab and reduces vigor. | abstract the goal of this research was to improve scab resistance of apple by transformation with genes encoding chitinolytic enzymes from the bio-control organism trichoderma harzianum. the endochitinase gene, as cdna and genomic clones, was transferred into apple cv. marshall mcintosh by agrobacterium-transformation. a total of 15 lines were identified as transgenic by nptii enzyme-linked immunosorbent assay and polymerase chain reaction and confirmed by southern analysis. substantial differen ... | 2000 | 18944574 |
| the mycoparasite ampelomyces quisqualis expresses exga encoding an exo-beta-1,3-glucanase in culture and during mycoparasitism. | abstract ampelomyces quisqualis, a mycoparasite of fungi causing powdery mildews, exhibited high levels of extracellular exo-beta-1,3-glucanase activity in culture compared with neurospora crassa and gliocladium roseum. a. quisqualis culture filtrates affected powdery mildew caused by sphaerotheca fusca in a manner indicative of cell wall degradation, as determined by microscopic examination. a gene encoding an exo-beta-1,3-glucanase in a. quisqualis, designated exga, was isolated and sequenced. ... | 1999 | 18944674 |
| increased antifungal activity of trichoderma harzianum transformants that overexpress a 33-kda chitinase. | abstract transformants of the biocontrol agent trichoderma harzianum strain cect 2413 that overexpressed a 33-kda chitinase (chit33) were obtained and characterized. strain cect 2413 was cotransformed with the amds gene and its own chit33 gene under the control of the pki constitutive promoter from t. reesei. southern blotting indicated that the chit33 gene was integrated ectopically, mostly in tandem. some transformants showed the same restriction pattern, indicating preferable sites of integra ... | 1999 | 18944767 |
| molecular phylogenetic analyses of biological control strains of trichoderma harzianum and other biotypes of trichoderma spp. associated with mushroom green mold. | abstract a polymerase chain reaction-amplified dna containing the internal transcribed spacer (its)-1, 5.8s, and its-2 regions of the nuclear ribosomal dna transcriptional unit was sequenced for 81 isolates of trichoderma spp. associated with mushroom culture or used for biological control of plant pathogens. phylogenetic analyses revealed that the biocontrol isolates were more closely related to an isolate of t. harzianum biotype 1 (th1) than to the aggressive biotypes 2 and 4. th1 has been iso ... | 1999 | 18944776 |
| ecological studies of transformed trichoderma harzianum strain 1295-22 in the rhizosphere and on the phylloplane of creeping bentgrass. | abstract a beta-glucuronidase (gus) reporter gene and a hygromycin b (hygb) phosphotransferase gene were integrated separately into the trichoderma harzianum strain 1295-22 genome, using biolistic transformation. the mycelial growth and biocontrol ability of the transformed strains did not differ from that of the original strain. the transformed gus(+)-kanamycin-resistant (gus(+)kan(r)) strains were used to monitor growth and interactions with rhizoctonia solani on creeping bentgrass plants. the ... | 1998 | 18944981 |
| incorporation of weather forecasting in integrated, biological-chemical management of botrytis cinerea. | abstract a strategy for integrated biological and chemical control of botrytis cinerea in nonheated greenhouse vegetables was developed. the biocontrol agent used was a commercial preparation developed from an isolate of trichoderma harzianum, t39 (trichodex). decisions concerning whether to spray the biocontrol agent or a fungicide were made based on a weather-based disease warning system. the integrated strategy (botman [short for botrytis manager]) was implemented as follows: when slow or no ... | 1997 | 18945177 |
| cloning and characterization of the thcut1 gene encoding a cutinase of trichoderma harzianum t34. | the cloning and characterization of the thcut1 gene, which encodes a cutinase protein of the biocontrol fungus trichoderma harzianum t34, is reported. cutinases, which are secreted enzymes that hydrolyse cutin, belong to a class of serine esterases able to hydrolyze fatty acid esters and emulsified triglycerides. the thcut1 gene was isolated by screening of a genomic dna library from est 2104, generated from a t. harzianum t34 cdna library constructed under mycoparasitic and nutrient stress cond ... | 2008 | 18987860 |
| thctf1 transcription factor of trichoderma harzianum is involved in 6-pentyl-2h-pyran-2-one production and antifungal activity. | we describe the cloning and characterization of the trichoderma harzianum thctf1 gene, which shows high sequence identity with a transcription factor gene of fusarium solani f. sp. pisi. in t. harzianum, disruption of the thctf1 gene by homologous recombination gave rise to transformants that in plate experiments did not show the yellow pigmentation observed in the wild-type strain. in several trichoderma spp. a yellow pigmentation and a coconut aroma have been related to the production of 6-pen ... | 2009 | 19007898 |
| cloning and heterologous expression of ss10, a subtilisin-like protease displaying antifungal activity from trichoderma harzianum. | trichoderma harzianum parasitizes a large variety of phytopathogenic fungi. trichoderma harzianum mycoparasitic activity depends on the secretion of complex mixtures of hydrolytic enzymes able to degrade the host cell wall. a gene (ss10) encoding a subtilisin-like protease was cloned from t. harzianum t88, a biocontrol agent effective against soil-borne fungal pathogens. the full-length cdna was isolated by 5' and 3' rapid amplification of the cdna ends. the coding region of the gene is 1302 bp ... | 2009 | 19025577 |
| an enzyme cocktail for efficient protoplast formation in aspergillus niger. | novozym 234 has been traditionally used to prepare protoplasts for genetic transformation of fungi. since it is no longer on the market, a new enzyme cocktail was defined to protoplast aspergillus niger. the cocktail consists of lysing enzymes from trichoderma harzianum, chitinase from streptomyces griseus and beta-glucuronidase from helix pomatia. | 2009 | 19041907 |
| effect of plant growth regulators on in vitro biological control of fusarium oxysporum by trichoderma harzianum (t8). | in this study the effect of two plant growth regulators (indolacetic acid, iaa and gibberellic acid, ga3) and also trichoderma harzianum (t8) on the phytopathogen fungus fusarium oxysporium (f15) was investigated. iaa and ga3 with 15 and 30 ppm concentration have no significant effect on t. harzianum (t8) growth. the biocontrol activity of t. harzianum on f. oxysporum was slightly decreased by the presence of iaa and/or ga3. addition of 40 ppm of ga3 to the culture medium of f. oxsporum increase ... | 2007 | 19090187 |
| identification of potential marker genes for trichoderma harzianum strains with high antagonistic potential against rhizoctonia solani by a rapid subtraction hybridization approach. | a rapid subtraction hybridization approach was used to isolate genes differentially expressed during mycelial contact between trichoderma harzianum (hypocrea lixii) and rhizoctonia solani, and could serve as marker genes for selection of superior biocontrol strains. putatively positive clones were evaluated by transcription analysis during mycelial contact with r. solani versus growth on glucose, and for their differential transcription between two strains with either strong or poor biocontrol c ... | 2009 | 19116716 |
| identification and investigation on antagonistic effect of trichoderma spp. on tea seedlings white foot and root rot (sclerotium rolfsii sacc.) in vitro condition. | the aim of this two years research work is to recognize bio control agents and its antagonistic efficiency on sclerotium rolfsii in vitro condition. sclerotium rolfsii sacc. is a causal agent of white foot and root rot disease in many plants. this fungi causing damage in tea nurseries of tea cultivated countries especially in iran. due to importance this disease in tea nurseries of iran and impossibility using of chemical control against this damaging agent in this research, five species of tric ... | 2008 | 19137869 |
| fatal trichoderma harzianum infection in a leukemic pediatric patient. | we report the repeated isolation for trichoderma.harzianum, a rare opportunistic pathogen from three sets of each of the following clinical samples; blood serum, skin lesions, sputum and throat of a pediatric all patient with neutropenia. the definition of invasive fungal infection requires evidence of the presence of fungal elements in tissue samples, in addition to the isolation of suspected etiologic agent in culture. however, invasive procedures are not always applicable due to several facto ... | 2009 | 19169948 |
| attempts to control fusarium root rot of bean by seed dressing. | in summer 2006, a root rot caused by fusarium oxysporum was observed in commercial farms on common bean (phaseolus vulgaris) on the cv billò and borlotto. a study was undertaken in order to evaluate the efficacy of different biological control agents applied as seed dressing. in the presence of a medium-high disease incidence, among the biocontrol agents tested, trichoderma harzianum t 22, bacillus subtilis qst 713, followed by pseudomonas chlororaphis, provided generally the best control. their ... | 2008 | 19226744 |
| mycorrhizal fungi and trichoderma harzianum as biocontrol agents for suppression of rhizoctonia solani damping-off disease of tomato. | the objective of the present work was to examine the interaction between arbuscular mycorrhizal fungus (amf) glomus intraradices and trichoderma harzianum in soil. soil application with t. harzianum or/and g. intraradices significantly reduced tomato seedlings damping-off incited by rhizoctonia solani. moreover, more pronounced disease suppression was obtained when both bioagents were applied together. application of t. harzianum to healthy or inoculated seedlings significantly increased phospho ... | 2008 | 19226759 |