Publications

mitochondrial atpase complex of aspergillus nidulans and the dicyclohexylcarbodiimide-binding protein.	the dicyclohexylcarbodiimide-binding protein of aspergillus nidulans has been identified as the smallest subunit of the mitochondrial atpase complex, and has a molecular weight of approximately 8000. it is extractable from whole mitochondria and from the purified enzyme in neutral chloroform/methanol, contains 30% polar amino acids, and the n-terminal amino acid has been identified as tyrosine. using a double-labelling technique in the absence and presence of cycloheximide, followed by immunopre ...	1979	157278
adenosine triphosphatase of aspergillus nidulans: existence of isoenzymes of ca2+-atpase.	ca2+-atpase activity increased five- to six fold when the cells were subjected to growth at 37 degrees c in protein hydrolysate-supplemented media as compared to that of the cells grown in minimal media. one major isoenzyme and one minor isoenzyme were present in minimal-medium-grown cells while two major isoenzymes were present in the cells grown in protein-supplemented media. when the cells were subjected to heat stress (43 degrees c), they exhibited significantly decreased activity as compare ...	1979	158522
short-term tests for carcinogens and mutagens.		1979	159413
mechanism of action of monoketo-organomycin, cystaurimycin and their performic acid-oxidized modifications. ii. inhibition of mitochondrial atpase activity in aspergillus nidulans and identification of the performic acid-oxidized modifications-binding protein.		1979	159889
study of human dermatomycosis.		1979	160399
[growth and toxin formation of species of the genus aspergillus under varying temperature regimens of cultivation].		1979	160976
[control of aspergillus fumigatus infections during a clinical stay].		1979	161380
[mycoses of the sinus].		1979	162345
pathogens, their sources and control--part two.		1975	162781
keratomycosis in wisconsin.	candida albicans was the most common fungus responsible for mycotic keratitis in our series from a northern climate, as opposed to southern climates where other fungi were more common. pimaricin was effective in our patients with candida infections and in one patient with aspergillus infection that had been unresponsive to previous amphotericin b.	1975	162899
regulation of mannitol-1-phosphate dehydrogenase in aspergillus nidulans.		1975	163669
kynureninase-type enzymes of penicillum roqueforti, aspergillus niger, rhizopus stolonifer, and pseudomonas fluorescens: further evidence for distinct kynureninase and hydroxykynureninase activities.	the kynureninase-type enzymes of three fungi and one bacterium were isolated and examined kinetically for their ability to catalyze the hydrolysis of l-kynurenine and l-3-hydroxykynurenine. the phycomycete rhizopus stolonifer was found to contain a single, constitutive enzyme with km for l-3-hydroxykynurenine and l-kynurenine of 6.67 times 10-minus 6 and 2.5 times 10-minus 4 m, respectively. the ascomycetes aspergillus niger and penicillium roqueforti each contain an enzyme, induced by l-tryptop ...	1975	164432
properties of enzymes. v. catalase inhibition of glucose oxidase reaction.	the radical produced aerobically in the glucose oxidase reaction reduces the oxidated cytochrome c. the extent of reduction depends on the concentrations of substrate (glucose) and enzyme. superoxide dismutase purified from various sources does not inhibit the cytochrome c reduction, but catalase does, in proportion to its concentration. this inhibition, which might be utilized for quantitative catalase determinations, provides further evidence for the formation of h2o2 in the glucose oxidase re ...	1975	165074
carbohydrate content and regulation following injection of different glycogenolytic enzymes.	the effect of injection of glycogenolytic enzymes on tissue glycogen, blood glucose and plasma insulin was studied in mice. no effects were observed following phosphorylase, whereas the hydrolytic enzymes, alpha-amylase and acid amyloglucosidase depressed liver glycogen. in addition acid amyloglucosidase induced a decrease in blood glucose, a slight elevation of plasma insulin and a marked increase in tolbutamide-stimulated insulin release. at the doses given none of the enzymes affected muscle ...	1975	165077
biochemical method for mapping mutational alterations in dna with s1 nuclease: the location of deletions and temperature-sensitive mutations in simian virus 40.	s1 nuclease (ec 3.1.4.x), a single-strand-specific nuclease, can be used to accurately map the location of mutational alterations in simian virus 40 (sv40) dna. deletions of between 32 and 190 base pairs, which are at or below the limit of detectability by conventional electron microscopic analysis of heteroduplex dnas, have been located in this way. to map a deletion, a mixture of unit length, linear dna, prepared from the sv40 deletion mutant and its wild-type parent, are denatured and reannea ...	1975	165498
[phylogenetic study of hepatic microsomal glucokinases].		1975	166791
carbon catabolite repression in aspergillos nidulans.		1975	168071
improvements in the thin-layer chromatography of natural products. i. thin-layer chromatography of the aflatoxins.	during the period which has elapsed since the aflatoxins were first isolated, one of the main problems has been the separation of the individual aflatoxins in pure form from aflatoxin-containing extracts. this separation has been best effected by thin-layer chromatography, and in this paper we describe how some of the difficulties may be overcome by using an appropriate combination of solvent system and silica gel preparation. for the examination of aflatoxin-containing extracts from the mycelia ...	1975	168220
kinetics and morphology of glucose-limited cultures of moulds grown in a chemostat and on solid media.	the affinity (ks value) of geotrichum candidum for glucose determined from chemostate cultures was ca. 1 mg/1. ks values for glucose was also estimated from the radial growth rates of colonies of g. candidum and neurospora crassa grown on media solidified with agar or silica gel. an assessment is made of the use of colony radial growth rate to determine substrate affinities. the length of apical and intercalary hyphal comparte ments, internode length and the diameter of leading hyphaat the margi ...	1975	168829
mapping of mutational alterations in dna with s1 nuclease: the location of deletions, insertions and temperature-sensitive mutations in sv40.		1975	169094
variations in hybridization of rna from different mouse tissues and embryos to endogenous c-type virus dna transcripts.	several adult tissues, newborns, and embryos of uninfected balb/c mice were analysed for rna complementary to [3h]-dna transcripts synthesized from an endogenous type-c virus of balb/c 3t3. the technique of rna:dna hybridization was used and the extent of hybridization was measured by the use of a single-strand-specific nuclease (s-i), purified from aspergillus oryzae. virus-specific rna was detected in all adult and embryonic tissues tested. however, the rna extracted from tissues having higher ...	1975	169317
specificity of the s1 nuclease from aspergillus oryzae.	conditions are described for digesting single-stranded dna by s1 nuclease without introducing breaks in double-stranded dna. the enzyme is inhibited by low concentrations of various compounds of phosphate. under certain conditions s1 nuclease cleaves the strand opposite a nick in bacteriophage t5 dna; under other conditions, the enzyme cleaves a loop in one strand of heteroduplex lambdadna while leaving the opposite strand intact. s1 nuclease makes many single strand breaks in ultraviolet-irradi ...	1975	171268
a mutant of aspergillus nidulans defective in nad-linked glutamate dehydrogenase.	a mutation leading to partial loss of nad-linked ("catabolic') glutamate dehydrogenase does not affect the regulation of ammonium-repressible activities in aspergillus nidulans. this mutation has been used to show that nad-linked glutamate dehydrogenase does not normally participate in ammonium assimilation. a mutation leading to loss of nadp-linked ("anabolic') glutamate dehydrogenase has been used to show that nadp-linked glutamate dehydrogenase is not normally involved in glutamate catabolism ...	1975	172777
bronchial provocation tests in etiologic diagnosis and analysis of asthma.		1975	173214
sexual differentiation in aspergillus nidulans: the requirement for manganese and the correlation between phosphoglucomutase and the synthesis of reserve material.	aspergillus nidulans was completely devoid of fruit bodies when grown on manganese deficient cultures. this result was shown earlier to be due to a lack of alpha-1,3 glucan in the cell wall. several enzymes of carbon and nitrogen metabolism were investigated in an attempt to explain the absence of this reserve material. synthesis of glucose-6-phosphate dehydrogenase, phosphoglucoisomerase and aldolase, were not strongly affected by manganese deficiency. however, phosphoglucomutase showed only 60 ...	1975	173248
mutants of aspergillus nidulans lacking nicotinamide adenine dinucleotide-specific glutamate dehydrogenase.	ten mutants of aspergillus nidulans lacking nicotinamide adenine dinucleotide-specific glutamate dehydrogenase (nad-gdh) have been isolated, and their mutations (gdhb1 through gdhb10) have been shown to lie in the gdhb gene. in addition, a temperature-sensitive gdhb mutant (gdhb11) has been isolated. a revertant (designated r-5) of the mutant gdhb1 bears an additional lesion in the gdhb gene and has altered nad-gdh activity with altered km values for ammonia or ammonium ions and for alpha-ketogl ...	1976	173707
galactosaminogalactan from cell walls of aspergillus niger.	a new heteropolysaccharide has been isolated by alkaline extraction of hyphal walls of aspergillus niger nrrl 326 grown in surface culture. its composition by weight, as determined by paper and gas chromatography and colorimetric analyses, is 70% galactose, 20% galactosamine, 6% glucose, and 1% acetyl. two independent experiments have been used to ascertain copolymer structure: permeation chromatography in 6 m guanidinium hydrochloride, with controlled-pore glass columns of two fractionation ran ...	1976	173713
induction of liver tumors by aflatoxin b1 in the tree shrew (tupaia glis), a nonhuman primate.	the epidemiological studies suggest that aflatoxins, the toxic metabolites of the ubiquitous mold aspergillus flavus, may play a significant role in the evolution of hepatocellular carcinoma in man in certain geographic areas of the world. to ascertain their carcinogenicity in nonhuman primates, we have administered highly purified aflatoxin b1, intermittently in the diet at 2 ppm, to 10 female and 8 male tree shrews. the tree shrew (tupaia glis) is a nonhuman primate occurring throughout southe ...	1976	174805
reaction of fungal ceramides containing alpha-hydroxy acids with the periodate-schiff reagents.		1976	175082
[bronchopulmonary aspergilloma in children and bronchogenic cyst. a new case].	the authors report a new case of aspergilloma developing in an intra-pulmonary bronchogenic cyst in a girl aged 10 years. a cure was obtained after surgical excision. the possibility of serious complications, such as hemoptysis, abscess formation and spread of the aspergillus, has two consequences; the first is the utility of regular supervision of any intra-pulmonary air cavity and, eventually, surgical removal. the second is the necessity of operation as soon as the diagnosis of aspergilloma h ...	1975	175446
aflatoxin inhalation and alveolar cell carcinoma.		1976	175881
three genes determine the carboxin sensitivity of mitochondrial succinate oxidation in aspergillus nidulans.		1975	178574
possible effects of aflatoxin consumption by man.		1976	178583
the effect of glucose and manganese on adenosine-3',5'-monophosphate levels during growth and differentiation of aspergillus nidulans.	the role of cyclic adenosine monophosphate (camp) during growth and development of aspergillus nidulans was investigated. in normal cultures the highest amount of camp, expressed on a dry weight basis, was found after 24 h of growth when still more than 5% glucose was present in the medium. after depletion of the medium even a slight fall in camp was noted. glucose concentrations ranging from 0.5-12% resulted in a slight decrease in the amount of camp as measured after 24 h of growth. cultures w ...	1976	179489
mannitol production in fungi during glucose catabolism.	the levels of phosphofructokinase (ec 2.7.1.11) and mannitol-1-phosphate dehydrogenase (ec 1.1.1.17) have been determined in a number of mucor and penicillium species. mannitol-1-phosphate dehydrogenase was found in only one species of mucor, mucor rouxii, and this with a specific activity much lower than that found in penicillium species. all of the fungi tested in the ascomycetes class exhibited mannitol-1-phosphate dehydrogenase activity. interference from both mannitol-1-phosphate dehydrogen ...	1976	179688
partial purification and some properties of oxalacetase from aspergillus niger.	1. oxalacetase from asperigillus niger was found to be an inducible enzyme, the induction being dependent not only on neutralisation of the acidic growth medium but also on the presence of carbonate. an explanation is proposed. 2. three methods were established for the quantitative determination of oxalacetase activity. these are based on the determination of the product acetate, on the absorbance of oxaloacetate and on coupling the hydrolysis of oxaloacetate to the oxidation of malate by nad in ...	1976	179820
effect of periodate oxidation on the structure and properties of glucose oxidase.	in order to elucidate the molecular structure of glucose oxidase (beta-d-glucose: oxygen 1-oxidoreductase, ec 1.1.3.4) and the roles of its carbohydrate moiety, chemical, physiochemical and immunological experiments were performed with enzyme samples before and after periodate oxidation. hydrodynamic parameters indicated that the native enzyme was a globular protein with values of 1.21 for the frictional ratio and 43 a for the stokes radius. the enzyme contained about 12% carbohydrate by weight, ...	1976	182278
regulation by zinc and adenosine 3',5'-cyclic monophosphate of growth and citric acid accumulation in aspergillus niger.	the citric acid fermentation by aspergillus niger is divided into two consecutive phases, growth phase when the cells proliferate but do not accumulate citrate, followed by an accumulating phase when they excrete citrate but do not proliferate. the phase alternative was controlled by the concentration of zinc: high zinc (2 mum) maintained growth phase, and a zinc 'deficiency' apparently signaled the ransition to the accumulating phase. cyclic amp affected the rates of growth and acidogenesis whe ...	1976	183882
silica gel medium to detect molds that produce aflatoxin.	a chemically defined nutrient solution containing tartaric acid was made solid by mixing it with an alkaline silicate solution. production of aflatoxin by asperfilli growing on the silica gel medium coincided with the presence of a blue fluorescent area surrounding colonies, as observed under long-wave ultraviolet light. presence of aflatoxin in the medium was confirmed by drying the gels, extracting them with chloroform-methanol, and examining extracts for fluorescent materials by viewing them ...	1976	185957
quality control for fermented meats.		1976	187566
[phospholipase c of fungi and staphylococci].	the activity of phospholipase was studied in the cultural broth and cell extract of 112 strains of fungi and yeasts. the endoenzyme was detected in 19 strains of mycelial fungi, the exoenzyme was found in mucor hiemalis 50 and aspergillus niger 117. phospholipase c of m. hiemalis was purified and compared to phospholipase of staphylococci. the values of km are 8.9 and 1.07 mm, respectively, for the fungal and staphylococcal enzymes.	1976	187904
the molecular mechanistic and immunological properties of amine oxidases.		1976	187928
studies on the phyllosphere microflora of tapioca (manihot utilissima pohl).	studies on the phyllosphere microflora of tapioca (manihot utilissima pohl.) revealed that incidence of bacteria, fungi, and actinomycetes is indipendent of the variety, but dependent on the age. the bacteria and fungi increased with increasing age of the leaf and were more on mature leaves, whereas no such difference was noticed in the occurrence of actinomycetes. the large majority of the isolates, that were gram-negative bacteria, was also amino acid-requiring. the chromogenic types occurred ...	1976	189529
[microbial resistance to formaldehyde. i. comparative quantitative studies in some selected species of vegetative bacteria, bacterial spores, fungi, bacteriophages and viruses].	the resistence of different microorganisms to formaldehyde was determined. as test objects served gram-negative and gram-positive vegetative germs (klebsiella pneumoniae, pseudomonas aeruginosa, salmonella paratyphi-b, staphylococcus aureus, streptococcus faecalis), bacterial spores (bacillus cereus, bacillus pumilus, bacillus stearothermophilus, bacillus subtilis), fungi (aspergillus niger, candida albicans), bacteriophages (escherichia coli phages, t1, t2, t3), and viruses (adenovirus, poliomy ...	1976	190825
production of aflatoxin b1 and g1 by aspergillus parasiticus on silica gels.	silica gels were prepared by acidifying alkaline silicate solutions with phosphoric or tartaric acid. various combinations of glucose, sucrose, yeast extract, and salts were included in the gels an nutrients. maximum production of aflatoxins b1 and g1 occurred when silica gel (0.4 to 0.5 cm deep in a petri dish) containing 20% sucrose and 2% yeast extract, and gelled with tartaric acid, was inoculated with approximately 120 to 12000 spores of aspergillus parasiticus per plate; and plates were in ...	1977	193316
immunosuppression and eye disease. first vail lecture.	several viral, fungal, and protozoal diseases of the eye are significantly associated with immunologic deficiencies. of the viral agents, cytomegaly and herpes simplex and zoster cause a discrete necrotizing retinopathy that has the characteristics of vascular occlusion. measles may result in a delayed retinopathy that is predominantly macular and associated with subacute progressive encephalopathy. of the fungal agents, candida and aspergillus are apt to involve the eye, beginning as choroidal ...	1977	194482
on the oligomerisation of periodate-oxidized glucose oxidase.		1977	196932
[effect of low molecular weight basic proteins on mannose transport in aspergillus niger microsomes].	basic proteins of low molecular weight activate the transfer of mannose to endogenous glycoprotein acceptors in microsomal membranes of aspergillus niger. the enhancement of mannosyltransferase activity is linked to the activation of the transport of mannose across the membrane. the role of these polycationic proteins on the membrane permeability is discussed.	1977	196935
the mechanism of action of citrinin on rabbit kidney alkaline phosphatase activity in vivo.	the effect of citrinin poisoning on rabbit kidney alkaline phosphatase was investigated. after seven days administration of citrinin (2 mg/kg body weight daily) the animals were sacrificed and the level of enzymes estimated in serum and kidney. serum enzymes showed no variation in activity in the citrinin-treated animals, but in kidney, alkaline phosphatase activity decreased significantly. the decreased activity was mainly associated with the cytoplasmic fraction and in fractions ib and ii. the ...	1977	197068
studies on the respiratory system of aspergillus oryzae. v. some properties of the respiratory system of mitochondria from mycelia grown in the presence of chloramphenicol.	presence of chloramphenicol in the growth medium for mycelia of aspergillus oryzae was without effect on the oxidative activity, respiratory control, or p/o ratio of isolated mitochondria. the mitochondria oxidized krebs cycle intermediates even in the presence of cyanide at the concentration markedly inhibiting the normal mitochondrial oxidation. however, the p/o ratio during the mitochondrial oxidation decreased by about 1.0 on addition of cyanide. the c-type cytochromes, shown to occur in lar ...	1977	199770
inside 45s ribonucleic acid.		1977	202281
effect of growth temperature on the cell constituents of aspergillus nidulans.		1977	202564
regulation of aflatoxin biosynthesis. 1 comparative study of mycelial composition and glycolysis in aflatoxigen and nonaflatoxigenic strains.	a comparative biochemical study of an aflatoxigenic strain aspergillus parasiticus nrrl 3240 and a nonaflatoxigenic strain a. flavus nrrl 3237 was carried out in order to have a better idea of regulation of aflatoxin biosynthesis. the results obtained revealed continuous primary metabolic activity (protein synthesis) in the nonaflatoxigenic strain while the aflatoxigenic stain showed inhibition of protein and nucleic acid synthesis. the aflatoxigenic strain showed higher levels of oxygen uptake, ...	1977	204853
purification and some properties of nad-degrading purine nucleosidase from aspergillus niger.	an enzyme which degrades nad at the adenine-ribose linkage has been purified from the mycelial extract of aspergillus niger. nadp, deamido-nad, and purine nucleosides and nucleotides were also susceptible to the hydrolytic cleavage. pyrimidine- and nicotinamide-ribose linkages were not attacked. the substrate specificity showed that the enzyme may be classified as a n-ribosyl-purine ribohydrolase (ec 3.2.2.1). the enzyme had a maximum activity in the ph range of 4.0-4.5 toward nad. the km value ...	1978	208724
high incidence of hepatic tumours in rats fed mouldy rice contaminated with aspergillus versicolor containing sterigmatocystin.		1978	208934
a novel phosphodiesterase from aspergillus niger and its application to the study of membrane-derived oligosaccharides and other glycerol-containing biopolymers.	a novel phosphodiesterase has been found in commercially available extracts of aspergillus niger and has been partially purified by fractionation with acetone and chromatography on carboxymethylcellulose. the enzyme attacks glycerophosphodiester bonds with the liberation of free glycerol only. the synthetic substrate glucose 6-phospho-sn-1'(3')-glycerol is hydrolyzed with production of equivalent amounts of free glycerol and glucose 6-phosphate. similarly, the enzymic hydrolysis of sn-glycero-3- ...	1978	212432
primary cytomegalovirus and opportunistic infections. incidence in renal transplant recipients.	thirty-five renal allograft recipients were studied concerning the relationship between cytomegalovirus (cmv), herpes simplex virus (hsv), and opportunistic bacterial and fungal infections. the incidence of opportunistic infections was determined for patients whose tests prior to transplantation were seronegative in complement fixation and indirect hemagglutination assays of cmv antibody and for those patients whose tests were seropositive. among the six seronegative patients with seronegative t ...	1978	213620
[paramagnetic properties of the conidial pigments of aspergillus niger and penicillium notatum fungi isolated from the mesosphere].	the conidia of aspergillus niger and penicillium notatum detected in the upper atmospheric layers of the earth (58--77 km) were found to contain stable paramagnetic centres (pmc) at concentrations of 0.2 x 10(18) and 1.6 x 10(18) per gram of dry biomass, respectively. aspergillin, the black pigment of asp. niger, was shown to have (0.1--0.6) x 10(18) pmc per gram of dry substance (depending on the fraction). stable paramagnetism of the conidial pigments with respect to their active protecting ac ...	1978	213700
mechanism of aromatic hydroxylation : the kinetic mechanism & the involvement of superoxide anions in the reaction catalyzed by m-hydroxybenzoate-4-hydroxylase from aspergillus niger (ubc-814).		1978	214394
[infectious causes of perinatal mortalities in ruminants (author's transl)].	the advantages and disadvantages of the different diagnostic techniques e.g. pathological and microbiological studies, immunoglobulin and specific antibody determinations and fluorescent antibody studies in relation to these mortalities are discussed. the most important pathological lesions in the placentas and foetuses are described.	1978	215769
specific site of action for single-strand specific nuclease on the double-stranded circular dna intermediates of an avian rna tumor virus.	circular viral dna intermediates obtained from the quail tumor line, qt6, at 1 day after infection, were opened at one specific location by the single-strand specific nuclease, s1, of aspergillus oryzae. this site was no longer accessible to the s1 nuclease when circles were first opened at another location with a restriction endonuclease.	1978	215777
[effect of the medium makeup on the synthesis of exoproteases by the fungus, aspergillus candidus strain 70].		1979	223677
phosphatase production by microorganisms isolated from diverse types of soils.	qualitative and quantitative nature of phosphatases produced by various fungal, bacterial, and actinomycetes isolates from diverse soils was examined. soil fungi, particularly those belonging to the genera aspergillus and penicillium, were found to be most effective in producing phosphatases intra- and extracellularly. none of the 16 bacterial isolates produced acid phosphatase, but most of them did produce neutral phosphatase. actinomycetes produced only negligible quantities of phosphatases.	1979	224622
the preparation and chemical composition of fractions from aspergillus fumigatus wall and protoplasts possessing antigenic activity.	a detergent-soluble fraction was prepared from the fragmented wall of aspergillus fumigatus mycelium using the non-ionic detergent triton x-100, and a wall-free extract was prepared from the same source in the form of protoplasts, released by a lytic enzyme system from trichoderma harzianum. these extracts were examined by polyacrylamide gel electrophoresis and their detailed chemical composition was established. they were compared with the water-soluble fraction prepared from total mycelium, wh ...	1979	225408
esr characterization of chitins and chitosans.		1979	226089
[effect of the toxic metabolites of the fungus, aspergillus flavus, on the functional activity of the large intestine].		1979	228515
[characterization of mitochondria of aspergillus niger from mycelium growing in surface and submerged culture conditions (author's transl)].	this paper deals with studies on tightly coupled mitochondria present in the active citric acid producing mycelium of aspergillus niger growing in surface and submerged culture. a special homogenizer had been used for rapid extraction of mitochondria. observation in the electron microscope indicated that some of the isolated mitochondria were probably damaged during preparation. nevertheless, the crude mitochondrial fraction was capable of coupling phosphorylation to the oxidation of three diffe ...	1979	232600
assay of deoxyribonucleic acid homology using a single-strand-specific nuclease at 75 c.	we investigated the conditions under which a crude preparation of endonuclease s1 gives maximal hydrolysis of denatured deoxyribonucleic acid (dna) while giving minimal hydrolysis of native dna. the hydrolysis was measured by filtering and determining the acid-insoluble reaction product using 3h-labeled substrates. we also investigated various parameters in making this measurement. under appropriate conditions (in 1 mm znso-4, 0.168 m nacl at ph 4.8) denatured dna is hydrolyzed within 3% of comp ...	1975	234416
the structural gene for nadp l-glutamate dehydrogenase in aspergillus nidulans.	a total of 41 mutants lacking nadp l-glutamate dehydrogenase (nadp-gdh) activity have been studied. all the mutations were located at the gdha locus within 0-1% recombination of gdhai. two mutants, gdhai and gdha2, out of five examined, produced cross-reacting material which neutralized nadp-gdh anti-serum. the mutant gdha9 has altered km values for all five substrates: ammonium, alpha-ketoglutarate, l-glutamate, nadph and nadp. the mutant gdha20 had temperature-sensitive growth, abnormal ammoni ...	1975	234511
metabolism of glutamic acid in aspergillus ochraceus during the biosynthesis of ochratoxin a.	the uptake and utilization of glutamic acid in the biosynthesis of ochratoxin a by aspergillus ochraceus were studied. uniformly labeled l[14c]glutamic acid was incorporated into both the phenylalanine and isocoumarin moieties of ochratoxin a. penicillic acid was also labeled. during the early stages of development, the amino acid was used mainly for the synthesis of ribonucleic acid and protein. a portion of glutamic acid was oxidized and was recovered as metabolic 14co-2. the initial uptake v ...	1975	234718
analysis of results of catheter tip cultures in open-heart surgery patients.	a statistical analysis of 628 consecutive catheter tip cultures is presented. all were from patients undergoing open-heart surgery. the previously noted effects of early removal are shown to be significant. the possible effects of stasis, flushing, handling, and place of insertion are discussed. the unresolved significance of isolations of aerobic sporing bacilli is noted, and a decreasing incidence of postoperative infective endocarditis in the same group of patients is an encouraging sign.	1975	235801
isolation and purification of blasticidin s deaminase from aspergillus terreus.	an enzyme catalyzing the deamination of the cytosine moiety of blasticidin s was extracted from a fungal strain that belongs to aspergillus terreus. the enzyme was purified with ammonium sulfate fractionation, sephadex g-100 column and deae cellulose column chromatography, followed by preparative polyacrylamide gel electrophoresis. blasticidin s deaminase could be separated easily from co-existing cytidine deaminase by deae column chromatography or gel electrophoresis, and preliminary study on t ...	1975	236272
fungal growth and acid production during fermentation and refermentation of organic acid treated corn silages.	chopped corn (35% dry matter) treated with either propionic, formic, 60% propionic plus 40% formic, or 80% propionic plus 20% acetic acids at 0, .5, 1, and 2%, was placed in polyethylene bags inside metal drums, and evacuated. during fermentation silages were sampled and temperatures determined. on day 40 of fermentation silages were placed in open containers at 25 c and were sampled during refermentation. all samples were analyzed for volatile and lactic acids, ph, and number and type of fungi. ...	1975	236333
biotin.		1975	236527
[specificity of the degradation and synthesis of dinucleoside monophosphates by rnaase c2 of asp. clavatus].	the ability of rnaase e2 to degradate dinucleoside moniphosphates and to form internucleotide bonds was studied. the compounds of the gpn type were found to be a good substrate for rnase c2. the ph optimum for gpc was 5.5 (acetate buffer) and the temperature optimum was 30 degrees c. the values of km and vmax on gpc, gpa, gpg and gpu were determined. the affinity of the substrates for the enzyme decreased in the sequence gpc greater than gpg greater than gpa greater than gpu. rnase c2 catalyze t ...	1975	236554
purification, properties and induction of a specific benzoate-4-hydroxylase from aspergillus niger (ubc 814).	an inducible benzoate-4-hydroxylase has been partially purified from crude extracts of the mycelial felts of aspergillus niger. this enzyme catalyzes the transformation of benzoate to p-hydroxybenzoate with equimolar consumption of nadph and o2. it requires tetrahydropteridine as a prosthetic group. the optimum activity was found at ph 6.2 with a km value at 30 degrees c of 1.6-10-minus 4 for nadph and 1.3-10-minus 4 m for benzoate. fe-2+ (iron) is required for the enzyme activity. the enzyme is ...	1975	236777
purification and properties of beta-galactosidase from aspergillus oryzae.	beta-galactosidase [ec 3.2.1.23] has been purified from a culture of aspergillus oryzae by 2-propanol fractionation, column chromatography on deae-sephadex a-50 and sephadex g-200. the preparation was homogeneous on ultracentrifugation and disc electrophoresis. the enzyme showed ph optima of 4.5 with onpg-1 as a substrate and 4.8 with lactose as a substrate. the stable ph range was from 4.0 to 9.0 and the optimum temperature was 46 degrees. the michaelis constants were 7.2 x 10-minus 4 m with on ...	1975	236999
purification of s1 muclease from takadiastase by affinity chromatography on single-stranded dna-acrylamide columns.	when s1 nuclease from takadiastase was partially purified according to previously reported methods, it showed a 10 to 15 fold increase in specificactivity. although such preparations were highly active on single-stranded dna, they had traces of activity on native dna and were contaminated by t1-rnase. the s1 enzyme was further purified by a single step of affinity chromatography on single-stranded dna-acrylamide column to a final purification of 275-fold. this preparation was free of t1-rnase an ...	1975	237256
[action of migillin on its own producer, asp. fumigatus].	addition of migillin to the nutrient medium at early stages of the fungus development induced at one hand inhibition of the fungus growth during the first 24-48 hours and stimulation of the biomass accumulation during the following hours of the development and on the other hand it induced inactivation of migillin added and inhibition of production of the "own" antibiotic. the migillin effect was accompanied by a delay in liberation of the substance with adsorption at 260nm from the mycelium.	1975	237468
patterns of action of glucoamylase isozymes from aspergillus species on glycogen.	glucoamylase isozymes from black aspergillus species have been freed of all traces of alpha-amylase by chromatography on bio-gel p-100, as evidenced by limited hydrolysis of oxidized amylose. glucoamylase i retains its ability to hydrolyze rabbit-liver glycogen rapidly. by contrast, glucoamylase ii hydrolyzes glycogen slowly, and addition of alpha-amylase to glucoamylase ii does not enhance its activity toward glycogen. these results indicate that alpha-amylase is not involved in hydrolysis of g ...	1975	237628
culture medium alkalinization by dermatophyes. (influence of time and temperature of incubation).	95 dermatophyte strains (12 of trichophyton mentagrophytes, 12 of t. tonsurans, 11 of t. rubrum, 12 of t. megninii, 12 of t. violaceum, 2 of t. schoenleinii, 1 of t. soudanense, 12 of m. canis, 8 of microsporum gypseum, 1 of m. ferrugineum and 12 of epidermphoyton floccosum). 1 of aspergillus niger, 1 of a. ochraceus, 1 of paecilomyces sp., 1 of penicillium sp. and 1 of candida albicans were grown in sabouraud liquid medium for the study of ph variation over 6 weeks at room temperature and after ...	1975	238130
effects of trace metals on the production of aflatoxins by aspergillus parasiticus.	certain metals added as salts to a defined basal culture medium influenced the level of aflatoxin production by aspergillus parasiticus in the low micrograms-per-milliliter range of the added metal. in many cases no change or a relatively small change in mat weight and final ph of the medium accompanied this effect. with zinc at added levels of 0 to 10 mug/ml in the medium, aflatoxin increased 30-to 1,000-fold with increasing of zinc, whereas mat weight increased less than threefold. at 25 mug o ...	1975	238471
purification and characterization of aspergillus niger exo-1,4-glucosidase.	a specific exo-1,4-glucosidase (1,4-alpha-d-glucan glucohydrooase, ec 3.2.1.3) from aspergillus niger has been partially purified and subsequently characterized by biochemical, physico-chemical and optical methods. molecular sieve chromatography yields an enzyme with maximal activity at ph 4.2-4.5 close to its isoelectric point. reduction and carboxymethylation leads to complete loss of activity and o-acetylation of 3 of the 13 tyrosine residues results in loss of 20 % of the activity. sodium do ...	1975	238601
studies on the interaction between streptomyces pepsin inhibitor and several acid proteinases by means of a zinc(ii)-dye complex as a probe.	the zinc(ii) complex of pyridine-2-azo-p-dimethylaniline is bound to several acid proteinases, at ph 5.0, accompanied by a change is the visible absorption spectrum. streptomyces pepsin inhibitor, which was discovered by satoi and murao (satoi, s. and murao, s. (1970) agric. biol. chem. 34, 1265-1267 and satoi, s. and murao, s. (1971) agric. biol. chem. 35, 1482-1487), is also bound to acid proteinases. spectrophotometric studies with ten acid proteinases from different sources have revealed tha ...	1975	238604
the microbial colonization of some woods of small dimensions buried in soil.	several species of wood veneer, including some in a green undried state, were buried in various soils, and at intervals the colonists were isolated and identified. in addition, veneers were deteriorated for different periods of time, sterilized, and then reburied in the same soil. isolates were obtained before sterilization and compared with those found afterwards. in each case the colonization involved a small number of microfungi and, because similar species were repeatedly isolated, an absenc ...	1975	238728
the inhibition of bacterial growth by ochratoxin a.	a series of bacterial species was examined for their sensitivity to ochratoxin a. only grampositive bacteria could be inhibited, generally at a ph lower than 7.0. bacillus subtilis did not show any reduction of growth rates in presence of ochratoxin a, but had a prolonged lag phase. with staphylococcus pyogenes var. aureus and streptococcus faecalis, a prolonged lag phase and a reduction of the growth rate was observed. most sensitive was streptococcus faecalis in the exponential-growth phase. t ...	1975	238733
malonate as a precursor in the biosynthesis of aflatoxins.	incorporation of [i-14c]acetate and [2-14c]malonate into aflatoxins by resting mycelia of aspergillus parasiticus resuspended in different buffers was studied. a decrease in ph from 5-8 to 2-8, as well as addition of edta, markedly stimulated the incorporation of malonate but the effect on acetate incorporation was less pronounced. mycelia took up comparatively more acetate than malonate, but more malonate (4-3%) entering mycelia was incorporated into aflatoxins than was acetate (1-6%). furtherm ...	1975	239093
inhibition of growth of aspergillus flavus and trichoderma viride by peanut embryos.	growth of aspergillus flavus and trichoderma viride on agar media was inhibited around embryos of green peanut seeds but not around embryos of cured seeds, intact peanut seeds, or testae. both fungi were able to colonize intact seeds and testae. substances inhibitory to a. flavus and t. viride were extracted with acetone from cotyledons of freshly harvested peanut seeds. four compounds inhibitory to a. flavus were detected in crude acetone extracts. three of the compounds demonstrated properties ...	1975	239349
[randomised investigations of some tyrolean swimming pools for the presence of trichomonas vaginalis and pathogenic fungi (author's transl)].	trichomonads were not demonstrated either microscopically or by culture in water samples from 15 public open air swimming pools in the tyrol. on the other hand, a small number of pathogenic fungi (candida albicans, trichophyton mentagrophytes, tr. verrucosum) were cultured from pool water with a free chlorine content of less than 0.35 mg/l (dpd 1). dermatophytes could also be demonstrated by cultures of the coatings on the surfaces of tiles in the showers, and of pool surrounding-and cabin tract ...	1975	239503
aspergillus oryzae acid proteinase. purification and properties, and formation of pi-chymotrypsin.	an acid proteinase from aspergillus oryzae was isolated from a commercial powder by successive (nh4)2so4 fractionation, acetone precipitation, and ion-exchange chromatography on phosphate- and deae-cellulose columns. the purified enzyme was found to be homogeneous by ultracentrifuge-sedimentation analysis (s20, w equal 3.63s), but electrofocusing in polyacrylamide gels and electrophoresis at ph 3.2 revealed that it consists of two very closely migrating bands. no difference in the amino acid com ...	1975	239702
spider mite webbing. iii. solubilization and amino acid composition of the silk protein.		1975	239809
[mycotoxins in foods. iv. the influence of different packing foils on the growth of aspergillus flavus and the formation of aflatoxins b1 and g1 from various kinds of sliced bread].		1975	239888
fermentative production of dextranase by aspergillus luchuensis inui.		1975	239903
purification and properties of a new ribonuclease from aspergillus saitoi.	from a commercial digestive produced from aspergillus saitoi, a ribonuclease [ec 3.1.4.23] having a molecular weight of 12,500 has been isolated in addition to the rnase reported previously, which had a molecular weight of 38,000. the enzyme was found to be homogeneous by chromatography on deae-cellulose, disc electrophoresis on polyacrylamide gel, and ultracentrifugation. the nh2-terminal amino acid was identified as glutamic acid. the amino acid composition indicated the presence of about 13 t ...	1975	239932
metabolism of aromatic compounds by fungi. kinetic properties and mechanism of 3-carboxy-cis,cis-muconate cyclase from aspergillus niger.	a preliminary investigation of the kinetic properties of 3-carboxy-cis,cis-muconate cyclase (ec 5.5.1.5) has been performed. the initial velocity of the reaction was shown to be proportional to the concentration of the enzyme in the assay system adopted and the apparent km was found to be 57 mum at ph 6.0 and 30 degrees c but at concentrations exceeding 70 mum, substrate inhibition was apparent. at ph 6.0 the ki for the substrate was 0.45 mm. plots of v and km against ph showed inflexions at ph ...	1975	240704
[studies on the reaction mechanism of a ribonuclease ii from aspergillus oryzae (author's transl)].	ribonuclease t2 was isolated from an aspergillus oryzae extract. in order to define the substrate specificity, the hydrolysis of a series of 2',3'-cyclic nucleotides was measured semiquantitatively. modifications in all positions of the bases are tolerated, as long as the base stays in the anti conformation or has a chance to return to it; bulky substituents at n-3 of the pyrimidine base lower the rate. so far the conclusion seems justified that the enzyme does not react with the substrates by s ...	1975	240766
on the physical properties and mechanism of action of arylsulfate sulfohydrolase ii from aspergillus oryzae.		1975	241293
diguanosine nucleotides of fungi that regulate rna polymerases isolated and partially characterised.		1975	241338
activity of endonuclease s1 in denaturing solvents: dimethysulfoxide, dimethylformamide, formamide and formaldehyde.		1975	241350
the regulation of nitrate reductase in the fungus aspergillus nidulans.		1975	241670