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nutrient utilization profile of saccharomyces cerevisiae from palm wine in tropical fruit fermentation.the nutrient utilization pattern of saccharomyces cerevisiae from palm wine was studied using tropical fruits as substrate. starter cultures were prepared by growing 15-18 h old stock cultures of the yeast in successively larger bottles containing pasteurized fruit must. microvinification, substrate utilization and assay of yeast activity were performed. soluble solute (ss) content of the juices ranged from 10-18 brix. pinapple must had the highest ss content (18 brix) while pawpaw had a low ss ...200415539927
antioxidant activities of various fruits and vegetables produced in taiwan.fruits and vegetables have been known to contain a variety of antioxidant components. it has been suggested that antioxidants may protect biomolecules from oxidative damage and therefore be associated with reduced risks of cardiovascular disease and certain cancer. the antioxidant abilities of various parts of eight common fruits and vegetables produced in taiwan were investigated, including tomato, guava, squash, tangerine, wax gourd, pineapple, chayote, and eggplant. squash, wax gourd, tomato, ...200415545051
pineapple juice as a negative oral contrast agent in magnetic resonance cholangiopancreatography: a preliminary evaluation.the quality of magnetic resonance cholangiopancreatography (mrcp) images is frequently degraded by high signal from the gastrointestinal tract. the aim of this study is to evaluate pineapple juice (pj) as an oral negative contrast agent in mrcp. preliminary in vitro evaluation demonstrated that pj shortened t(2) relaxation time and hence decreased t(2) signal intensity on a standard mrcp sequence to a similar degree to a commercially available negative contrast agent (ferumoxsil). electrothermal ...200415569640
towards sustainable use of potassium in pineapple waste.due to the 1997/98 haze problem in south-east asia and the increasing need for sustainable food production and development, the usual management of crop residues (including pineapple wastes) through burning is prohibited. as a result, the need for alternative uses of pineapple wastes in pineapple production has been emphasized. this study investigated an environmentally friendly means of recycling pineapple leaves for agricultural use. pineapple leaves were shredded and composted in a composting ...200415578124
occurrence and significance of cryptococcus neoformans in vegetables and fruits.cryptococcus neoformans was isolated from three of 437 (0.6%) samples of a wide variety of vegetables collected from a number of markets in delhi. the vegetables yielding c. neoformans were tomato, lycopersicon esculentum (1/70), 'vegetable sponge', luffa cylindrica (1/56) and brinjal, solanum melongena (1/55). only solitary samples of these three vegetables were found to be positive. in vitro studies at 25 degrees c revealed that a reference c. neoformans isolate readily colonized, as did candi ...200015597680
[osmolality of frequently consumed beverages].the objective of this work was to determine the osmolality of beverages frequently consumed by children and adolescents due to the scarce information available in our country. the samples were grouped as follows: milks; refreshments; beverages based on fruits, vegetables, cereals, and tubers; sport drinks; energizing drinks; oral rehydrating solutions; reconstituted drinks and infusions. a vapor pressure digital osmometer was used, five samples of each beverage from different lots were analyzed. ...200415602899
biofibers from agricultural byproducts for industrial applications.lignocellulosic agricultural byproducts are a copious and cheap source for cellulose fibers. agro-based biofibers have the composition, properties and structure that make them suitable for uses such as composite, textile, pulp and paper manufacture. in addition, biofibers can also be used to produce fuel, chemicals, enzymes and food. byproducts produced from the cultivation of corn, wheat, rice, sorghum, barley, sugarcane, pineapple, banana and coconut are the major sources of agro-based biofibe ...200515629854
an improved cryopreservation protocol for pineapple apices using encapsulation-vitrification.several modifications to the cryogenic protocols previously described for pineapple apices were performed using vitrification and encapsulation-vitrification. pregrowth of apices in sucrose-proline before loading significantly reduced the exposure duration to pvs2 and pvs3 required for successful cryopreservation. encapsulation and treatments with pvs3 at 0 degree c gave the highest survival before and after cooling. optimal conditions involved the encapsulation of pineapple apices in calcium al ...200515660167
purification, characterization, and antifungal activity of chitinases from pineapple (ananas comosus) leaf.three chitinases, designated pineapple leaf chitinase (pl chi)-a, -b, and -c were purified from the leaves of pineapple (ananas comosus) using chitin affinity column chromatography followed by several column chromatographies. pl chi-a is a class iii chitinase having a molecular mass of 25 kda and an isoelectric point of 4.4. pl chi-b and -c are class i chitinases having molecular masses of 33 kda and 39 kda and isoelectric points of 7.9 and 4.6 respectively. pl chi-c is a glycoprotein and the ot ...200515665484
lichen striatus in an adult following trauma.a 36-year-old man presented with a linear erythematous eruption along his right forearm and dystrophy of his right thumbnail. these changes had developed 2 weeks after his right thumb had been pricked by a pineapple leaf and evolved over a 6-week period. histopathology revealed features consistent with lichen striatus. he complained of mild intermittent pruritus, which responded to moderate-potency topical corticosteroids. at 12 months the skin eruption had improved, but the nail changes persist ...200515670174
applied k fertilizer use efficiency in pineapples grown on a tropical peat soil under residues removal.in malaysia, pineapples are grown on peat soils, but most k fertilizer recommendations do not take into account k loss through leaching. the objective of this study was to determine applied k use efficiency under a conventionally recommended fertilization regime in pineapple cultivation with residues removal. results showed that k recovery from applied k fertilizer in pineapple cultivation on tropical peat soil was low, estimated at 28%. at a depth of 0-10 cm, there was a sharp decrease of soil ...200515674449
pineapple juice for digestion of swamp eel viscera for harvesting infective-stage larva of gnathostoma spp.third-stage larvae were used as antigen in the diagnosis of gnathostomiasis in western blot analysis. normally, the larvae were obtained from digestion of eel's liver (fluta alba) by the enzyme pepsin. we used pineapple juice (ananus comosus) instead of enzyme pepsin in harvesting gnathostoma spinigerum third-stage larvae. the difference in recovered larvae numbers, between pineapple juice and pepsin, were not statistically significantly different (p>0.05). the larvae from pepsin and pineapple j ...200415691122
proteinase activity and stability of natural bromelain preparations.bromelain is a complex mixture of proteinases typically derived from pineapple stem. similar proteinases are also present in pineapple fruit. beneficial therapeutic effects of bromelain have been suggested or proven in several human inflammatory diseases and animal models of inflammation, including arthritis and inflammatory bowel disease. however, it is not clear how each of the proteinases within bromelain contributes to its anti-inflammatory effects in vivo. previous in vivo studies using bro ...200515710346
assessment of the anthelmintic effect of natural plant cysteine proteinases against the gastrointestinal nematode, heligmosomoides polygyrus, in vitro.we examined the mechanism of action and compared the anthelmintic efficacy of cysteine proteinases from papaya, pineapple, fig, kiwi fruit and egyptian milkweed in vitro using the rodent gastrointestinal nematode heligmosomoides polygyrus. within a 2 h incubation period, all the cysteine proteinases, with the exception of the kiwi fruit extract, caused marked damage to the cuticle of h. polygyrus adult male and female worms, reflected in the loss of surface cuticular layers. efficacy was compara ...200515727070
therapeutic use, efficiency and safety of the proteolytic pineapple enzyme bromelain-pos in children with acute sinusitis in germany.the therapeutic efficiency and safety of the proteolytic enzyme bromelaine obtained from pineapple (bromelain-pos, ursapharm gmbh, saarbrücken, germany) was evaluated in children under the age of 11 years diagnosed with acute sinusitis. data from 116 patients from 19 centres located across germany were analysed in a pharmacoepidemiological cohort study. patient cohorts were either treated with bromelain-pos (n = 62), in combination with bromelain-pos and standard therapies (n = 34), or with stan ...200615796206
modulation of murine tumor growth and colonization by bromelaine, an extract of the pineapple plant (ananas comosum l.).the antitumor and antimetastatic activities of the plant cysteine endoproteinase bromelaine were evaluated in a murine model. syngeneic sarcoma l-1 cells were incubated with bromelaine (after preceeding time and dosage kinetics) and subcutaneously; (s.c.) or intravenously; (i.v.) inoculated into balb/c-mice (n = 5 per experimental group) to induce local tumor growth or lung colonization. compared to non-protease incubated l-1 cells, local tumor growth and experimental lung metastasis decreased s ...200515796214
phytate and fiber content in thai fruits commonly consumed by diabetic patients.the amount of phytate and total dietary fiber of 6 popular tropical fruits eaten by diabetic patient residing in bangkok were studied. they were dragon fruit, durian, guava, longan, mango, and pineapple. fruit containing the highest content of total dietary fiber was guava which had about 2.7%. longan contained the lowest fiber content equal to 0.19%. the highest content of phytate was found in pineapple about 0.09% and the lowest content was in longan about 0.037%. it was noticed that longan co ...200415822538
bromelain as a treatment for osteoarthritis: a review of clinical studies.bromelain, an extract from the pineapple plant, has been demonstrated to show anti-inflammatory and analgesic properties and may provide a safer alternative or adjunctive treatment for osteoarthritis. all previous trials, which have been uncontrolled or comparative studies, indicate its potential use for the treatment of osteoarthritis. this paper reviews the mechanism of its putative therapeutic actions, those clinical trials that have assessed its use in osteoarthritis to date, as well as cons ...200415841258
the effects of ananas comosus l. leaves on diabetic-dyslipidemic rats induced by alloxan and a high-fat/high-cholesterol diet.the aim of this study is to demonstrate the effects of ananas comosus l. leaves on diabetic-dyslipidemic rats. hypoglycemic and hypolipidemic activities of the ethanolic extract of ananas comosus l. leaves (eeacl) were evaluated in normal and alloxan-induced diabetic rats by oral glucose tolerance test and an olive oil load test. anti-diabetic, anti-hyperlipidemic and anti-oxidative activities of eeacl were also investigated in diabetic-dyslipidemic rats induced by alloxan and a high-fat/high-ch ...200515844837
tissue distribution, synthesis stage, and ethylene induction of pineapple (ananas comosus) chitinases.we examined the tissue distribution, synthesis stage, and ethylene induction of three types of pineapple chitinase using chitinase activity gel and immunoblot analysis. type a (acidic class iii) exists in all tissues, while type b (weakly basic class i, which has strong antifungal activity) and type c (acidic class i) are localized mainly in the leaf and stem. in a pericarp, type a exists at all stages during fruit development, while type b and type c exist only at the early stage. synthesis of ...200515849431
susceptibility of the interchain peptide of a bromelain inhibitor precursor to the target proteases bromelain, chymotrypsin, and trypsin.bromein, a cysteine proteinase inhibitor from pineapple stem, is a unique double-chain inhibitor. the 27.5-kda precursor protein is processed by the removal of three interchain, two interdomain, and two terminal-flanking peptides, thus resulting in the release of mature isoinhibitors of approximately 6 kda. to characterize the processing of the interchain peptide thr15-ser-ser-ser-asp, we expressed a single-chain precursor with this peptide and monitored proteolytic cleavage by the target protei ...200515927893
treatment with oral bromelain decreases colonic inflammation in the il-10-deficient murine model of inflammatory bowel disease.bromelain is a mixture of proteinases derived from pineapple stem that is marketed in health food stores as a "digestive aid". orally administered bromelain was anecdotally reported to induce clinical and endoscopic remission of ulcerative colitis in two patients whose disease was refractory to multi-agent conventional medical therapy. however, the potential efficacy of bromelain in colitis has not yet been tested rigorously in either animals or humans. in this study, the clinical and histologic ...200515936249
fungal infections of fresh-cut fruit can be detected by the gas chromatography-mass spectrometric identification of microbial volatile organic compounds.there is a large and rapidly growing market for fresh-cut fruit. microbial volatile organic compounds indicate the presence of fungal or bacterial contamination in fruit. in order to determine whether microbial volatile organic compounds can be used to detect contamination before fruit becomes unmarketable, pieces of cantaloupe, apple, pineapple, and orange were inoculated with a variety of fungal species, incubated at 25 degrees c, then sealed in glass vials. the volatiles were extracted by hea ...200515954710
detection of exogenous citric acid in fruit juices by stable isotope ratio analysis.a new method has been developed for measuring the d/h ratio of the nonexchangeable sites of citric acid by isotope ratio mass spectrometry (irms). pure citric acid is transformed into its calcium salt and subsequently analyzed by pyrolysis-irms. the citric acid isolated from authentic fruit juices (citrus, pineapple, and red fruits) systematically shows higher d/h values than its nonfruit counterpart produced by fermentation of various sugar sources. the discrimination obtained with this simplif ...200515969486
pineapple juice labeled with gadolinium: a convenient oral contrast for magnetic resonance cholangiopancreatography.the aim of our study was to prepare in vitro a pineapple juice (pj) solution labeled with a minimal gadolinium concentration working as a negative contrast agent in heavily t2-weighted imaging and to assess that solution in vivo as a negative oral contrast agent for magnetic resonance cholangiopancreatography (mrcp). three pjs were compared in vitro according to their t2. increasing concentrations of gadolinium (gd)-dota in pj were assessed in vitro for t2 reduction. single-shot turbo spin echo ...200515999215
effect of ripe fruit pulp on the sensory and nutritive quality of ready-to-eat breakfast cereal produced from maize and soybean flours and cassava starch blends.the effects of various concentrations of different ripe fruit pulps on the sensory and nutritive quality of breakfast cereal were studied. the breakfast cereal was formulated using 1 kg composite flour (composed of 600 g maize flour and 400 g soy flour) in addition to 100 g cassava starch, 225 g sugar and 12 g salt. pineapple, pawpaw and banana ripe fruit pulps were added separately to the breakfast formulation at concentrations of 0, 100, 200, 300 and 400 g/kg composite flour. using sensory eva ...200516019313
growth and survival of escherichia coli o157:h7 in fresh tropical fruit juices at ambient and cold temperatures.the survival of escherichia coli o157:h7 in acidic foods and its enhanced survival in refrigerated acid foods are well documented. this prompted the present study to evaluate the growth potential of e. coli o157:h7 in fresh tropical fruit juices. the ph of the various juices ranged between 3.57 (pineapple) and 6.2 (avocado). samples were separately inoculated with a mixture of four e. coli o157:h7 strains at initial levels of approximately 10(3) cfu/ml, and stored at refrigeration (4 degrees c) ...200516019323
odor-active constituents in fresh pineapple (ananas comosus [l.] merr.) by quantitative and sensory evaluation.by application of aroma extract dilution analysis (aeda) to an aroma distillate prepared from fresh pineapple using solvent-assisted flavor evaporation (safe), 29 odor-active compounds were detected in the flavor dilution (fd) factor range of 2 to 4,096. quantitative measurements performed by stable isotope dilution assays (sida) and a calculation of odor activity values (oavs) of 12 selected odorants revealed the following compounds as key odorants in fresh pineapple flavor: 4-hydroxy-2,5-dimet ...200516041138
molecular characterization of a gender-linked dna marker and a related gene in mercurialis annua l.the dioecious mercurialis annua l. was used as a model plant to study some aspects of the molecular basis of sex determination in plants. we report in this paper the characterization of a previously identified male specific dna marker, opb01-1562, from diploid dioecious m. annua. the marker co-segregated with male sex in the progeny of hormonally feminized males. sequence analysis showed the presence of approximately 0.6 kb retrotransposon-like sequence at its 3' end. homologous sequences were i ...200516049676
kenneth frank baker--pioneer leader in plant pathology.kenneth f. baker (1908-1996) made major contributions to understanding diseases of ornamental plants, seed pathology, soil-borne plant pathogens, biological control, and history of plant pathology. his work set the stage for the success of today's ornamentals and nursery industries. his leadership and writings created the scientific framework for research and teaching on soil-borne plant pathogens and biological control. after b.sc. and ph.d. degrees from washington state university in 1930 and ...200516078875
bromelain improves decrease in defecation in postoperative rats: modulation of colonic gene expression of inducible nitric oxide synthase.ileus continues to be a common consequence of abdominal surgery, causing significant patient discomfort and often leading to more serious problems. the therapy available is limited, hence, ileus remains an important clinical problem. activation of inducible nitric oxide synthase (inos) directly modulates intestinal dysmotility after bowel manipulation and plays an essential role in initiating intestinal inflammation. nuclear factor (nf)-kappab is known to be a critical component of inos gene tra ...200616137711
pineappledb: an online pineapple bioinformatics resource.a world first pineapple est sequencing program has been undertaken to investigate genes expressed during non-climacteric fruit ripening and the nematode-plant interaction during root infection. very little is known of how non-climacteric fruit ripening is controlled or of the molecular basis of the nematode-plant interaction. pineappledb was developed to provide the research community with access to a curated bioinformatics resource housing the fruit, root and nematode infected gall expressed se ...200516202174
production of lactic acid and fungal biomass by rhizopus fungi from food processing waste streams.this study proposed a novel waste utilization bioprocess for production of lactic acid and fungal biomass from waste streams by fungal species of rhizopus arrhizus 36017 and r. oryzae 2062. the lactic acid and fungal biomass were produced in a single-stage simultaneous saccharification and fermentation process using potato, corn, wheat and pineapple waste streams as production media. r. arrhizus 36017 gave a high lactic acid yield up to 0.94-0.97 g/g of starch or sugars associated with 4-5 g/l o ...200516208461
investigation of the utility of pineapple juice and pineapple waste material as low-cost substrate for ethanol fermentation by zymomonas mobilis.the utility of the juice of rotten or discarded pineapples and the waste material of the production of pineapple juice as low-cost substrates for ethanol production by zymomonas mobilis was investigated. z. mobilis atcc 10988 produced 59.0 g.l(-1) ethanol in undiluted pineapple juice without nutritional supplementation and without the regulation of the ph while 42.5 g.l(-1) ethanol was obtained using a 125 g.l(-1) sucrose medium supplemented with 10 g.l(-1) yeast extract and mineral salts. ethan ...199916232532
stability of enterocin as-48 in fruit and vegetable juices.enterocin as-48 is a candidate bacteriocin for food biopreservation. before addressing application of as-48 to vegetable-based foods, the interaction between as-48 and vegetable food components and the stability of as-48 were studied. enterocin as-48 had variable interactions with fruit and vegetable juices, with complete, partial, or negligible loss of activity. for some juices, loss of activity was ameliorated by increasing the bacteriocin concentration, diluting the juice, or applying a heat ...200516245711
plaque and salivary ph changes after consumption of fresh fruit juices.the concept of health has prevailed for centuries and the dietary habits are apparently changing with modernization. "healthy eating" is now perceived to be important. the desirability of a healthful lifestyle has led to an increased consumption of juices. drinking large amount of fruit juice is frequently practiced these days and the consumption of these juices is further modified with behavioral habits such as swishing and frothing the drinks around the mouth, sucking frozen fruit juices, use ...200516302592
the influence of bromelain on platelet count and platelet activity in vitro.bromelain is a general name for a family of sulfhydryl-containing, proteolytic enzymes from the pineapple plant. the aim of the present study was to investigate the influence of bromelain on platelet count, platelet aggregation and platelet activity in vitro. blood samples were taken from the antecubital vein of 10 healthy male non-smokers. platelet count decreased after incubation with 2.5 and 5 mg bromelain/ml from 277 +/- 17 platelets/nl before to 256 +/- 21 and 247 +/- 19 platelets/nl after ...200616308185
characterization of the acidic and basic limbs of a bell-shaped ph profile in the inhibitory activity of bromelain inhibitor vi.bromelain inhibitor vi (bi-vi) is a cysteine proteinase inhibitor from pineapple stem and a unique two-chain inhibitor composed of two distinct domains. bi-vi's inhibitory activity toward the target enzyme bromelain is maximal at ph 4 and shows a bell-shaped ph profile with pka values of about 2.5 and 5.3. this ph profile is quite different from that of bromelain, which is optimally active around ph 7. in the present article, to characterize the acidic limb, we first expressed the recombinant in ...200616315142
effect of acute and chronic grapefruit, orange, and pineapple juice intake on blood lipid profile in normolipidemic rat.high fruit intake is known to be associated with reduced risk of coronary heart disease. our objective was to determine the effects of acute and chronic juice [grapefruit, orange, and pineapple] intake on plasma lipid profile and lipoprotein metabolism in normolipidemic rats.200516319784
bromelain exerts anti-inflammatory effects in an ovalbumin-induced murine model of allergic airway disease.bromelain, a clinically used pineapple extract and natural product, has reported anti-inflammatory and immunomodulatory activities. the purpose of this study was to determine the effect of bromelain treatment in an ovalbumin (ova)-induced murine model of allergic airway disease (aad).200516337164
sulfasalazine transport in in-vitro, ex-vivo and in-vivo absorption models: contribution of efflux carriers and their modulation by co-administration of synthetic nature-identical fruit extracts.sulfasalazine is characterised by low oral bioavailability. in this study, its intestinal transport characteristics were studied in an in-vitro, ex-vivo and in-situ system. the absorptive transport of sulfasalazine across caco-2 monolayers appeared to be lower than the secretory transport (p(app-abs) = 0.21 +/- 0.02 x 10(-6) cm s(-1) and p(app-secr) = 2.97 +/- 0.30 x 10(-6) cm s(-1), respectively). this polarity in transport of sulfasalazine was not mediated by p-glycoprotein (p-gp), as inclusio ...200516354400
[hypersensitivity to poppy seeds].hypersensitivity to poppy seeds is rare and it may develop due to immune (allergy) and non-immune reactions of fulminant course. two cases of hypersensitivity to poppy seeds are presented: in a 21-year-old woman and 32-year-old man in whom life-threatening symptoms and signs of anaphylaxis (ssa) developed after consumption of poppy seeds in various situations, e.g in the street after consumption of a roll with poppy seeds or a cake prepared on a moulding board on which poppy seeds were previousl ...200516425801
in vitro and in vivo anthelmintic efficacy of plant cysteine proteinases against the rodent gastrointestinal nematode, trichuris muris.extracts of plants, such as papaya, pineapple and fig, are known to be effective at killing intestinal nematodes that inhabit anterior sites in the small intestine, such as heligmosomoides polygyrus. in this paper, we demonstrate that similar in vitro efficacy also occurs against a rodent nematode of the large intestine, trichuris muris, and confirm that the cysteine proteinases present in the plant extracts are the active principles. the mechanism of action of these enzymes involved an attack o ...200616448585
blackberry (rubus spp.): a ph-dependent oral contrast medium for gastrointestinal tract images by magnetic resonance imaging.in this study, seven fruits have been tested on their magnetic properties, paramagnetic metal content and contrast enhancement in magnetic resonance imaging (mri) of phantom and in vivo. magnetic susceptibility was determined for the fruit pulps, as well as the contents of paramagnetic metals; iron, manganese and copper. the total content of these metals was 4.3, 8.6, 11.1, 10.9, 12.3, 8.3 and 29.3 mg/kg of fruit for plum, blueberry, apple (red), pineapple, beet, grape, blackberry, respectively, ...200616455409
does modification of olfacto-gustatory stimulation diminish sensory-specific satiety in humans?alimentary sensory pleasure is an important factor in ingestive behavior. renewal of olfacto-gustatory pleasure by introducing new foods or through seasoning of previously consumed food might increase intake.200616458336
sharing malaysian experience with the development of biotechnology-derived food crops.biotechnology-derived food crops are currently being developed in malaysia mainly for disease resistance and improved post harvest quality. the modern biotechnology approach is adopted because of its potential to overcome constraints faced by conventional breeding techniques. research on the development of biotechnology-derived papaya, pineapple, chili, passion fruit, and citrus is currently under way. biotechnology-derived papaya developed for resistance to papaya ringspot virus (prsv) and impr ...200516465992
proliferation potential of 18-month-old callus of ananas comosus l. cv. moris.differential effect of plant growth regulators and additives in proliferation of 18-month-old calli of ananas comosus l. cv. moris were assessed in vitro. the proliferation of callus relied on the growth regulators and additives. of the different auxins supplemented in the murashige and skoog (ms) media, 32.22 microm alpha-naphthaleneacetic acid (naa) gave the highest mean fresh weight of callus (46.817 g). medium supplemented with 2,4-dichlorophenoxyacetic acid (2,4-d) was inferior to naa, whil ...200616493521
pharmacokinetic study of p-coumaric acid in mouse after oral administration of extract of ananas comosus l. leaves.quantification of p-coumaric acid in mouse plasma following oral administration of ananas comosus l. leaves was achieved by reversed-phase high-performance liquid chromatography using a mobile phase of water-acetonitrile (82:18, v/v) and uv detection at 310 nm. the method was linear (determination coefficient, r2 = 0.9997) within the tested range (0.04-1.28 microg/ml). intra- and inter-day precision coefficients of variation and accuracy bias were acceptable (maximal cv value was 4.06% for intra ...200616506268
characterization of aroma compounds of chinese "wuliangye" and "jiannanchun" liquors by aroma extract dilution analysis.aroma compounds in chinese "wuliangye" liquor were identified by gas chromatography-olfactometry (gc-o) after fractionation. a total of 132 odorants were detected by gc-o in wuliangye liquor on db-wax and db-5 columns. of these, 126 aromas were identified by gc-mass spectrometry (ms). aroma extract dilution analysis (aeda) was further employed to identify the most important aroma compounds in "wuliangye" and "jiannanchun" liquors. the results showed that esters could be the most important class, ...200616569063
production of natural fruity aroma by geotrichum candidum.based on its aromatic potential, geotrichum candidum isolated from olive vegetation water was tested for the production of volatile compounds. when g. candidum was cultivated on media with glucose as the carbon source, flavor volatile compounds were produced and accumulated in the broth. fruity flavoring compounds (pineapple-like) such as esters and alcohols were analyzed by gas chromatography coupled to mass spectrometry, including ethyl esters of acetic acid and butyric acid, methyl-3-butan-1- ...200616632883
the effect of preservatives on the sterility of microorganisms introduced into different fruit juices.the aim of this study was to investigate the degree of sterility of some fruit juices post manufacture and to determine how effective the preservatives in them aid the killing of some microorganisms introduced into them.200616641872
certain physicochemical properties of pineapple stem colloids. 192816652573
relationship between nitrogen fertilization and chlorophyll content in pineapple plants. 193516653256
assimilation of ammonium and nitrate by pineapple plants grown in nutrient solutions and its effects on nitrogenous and carbohydrate constitutents. 193816653506
distribution of different nitrogen fractions, sugars and other substances in various sections of the pineapple plant grown in soil cultures and receiving either ammonium or nitrate salts. 193916653552
distribution of nitrogenous and carbohydrate fractions and other substances in exposed and covered pineapple sister shoots. 194016653627
effects of iron on the growth and ash constituents of ananas comosus (l.) merr. 194316653879
effects of iron on chlorophyllous pigments, ascorbic acid, acidity and carbohydrates of ananas comosus (l.) merr., supplied with nitrate or ammonium salts. 194416653903
effects of different amounts of potassium on growth and ash constituents of ananas comosus (l.) merr. 194516654011
effects of potassium on chlorophyll, acidity, ascorbic acid, and carbohydrates of ananas comosus (l.) merr. 194516654014
effects of iron on certain nitrogenous fractions of ananas comosus (l.) merr. 194616654029
effects of potassium on the nitrogenous constituents of ananas comosus (l.) merr. 194616654038
effects of nitrogen on growth and ash constituents of ananas comosus (l.) merr. 194616654042
effects of nitrogen on the nitrogenous fractions of ananas comosus (l.) merr. 194716654085
chlorophyll and protein interrelationships in ananas comosus (l.) merr. 194716654087
effects of nitrogen on chlorophyll, acidity, ascorbic acid, and carbohydrate fractions of ananas comosus (l.) merr. 194716654093
diurnal changes and growth rates as associated with ascorbic acid, titratable acidity, carbohydrate and nitrogenous fractions in the leaves of ananas comosus (l.) merr. 194816654147
note on flower formation in the pineapple induced by low night temperatures. 194816654160
growth and chemical composition of ananas comosus (l.) merr., in solution cultures with different iron-manganese ratios. 194916654234
manganese interference in the absorption and translocation of radioactive iron (fe) in ananas comosus (l.) merr. 195016654292
growth of ananas comosus (l.) merr. at different levels of mineral nutrition under greenhouse and field conditions. i. plant and fruit weights and absorption of nitrate and potassium at different growth intervals. 195016654323
physiological studies of the fruits of the pineapple [ananas comosus (l.) merr.] with special reference to physiological breakdown. 195116654359
growth of ananas comosus (l.) merr., at different levels of mineral nutrition under greenhouse and field conditions. ii. chemical composition of the tissues at different growth intervals. 195116654385
distribution of total soluble solids, ascorbic acid, total acid, and bromelin activity in the fruit of the natal pineapple (ananas comosus l. merr.). 195316654569
effect of maleic hydrazide on pineapple. 195616654842
pineapple chlorosis in relation to iron and nitrogen. 195616654866
evapotranspiration of pineapple in hawaii. 196516656150
phosphoenolpyruvate carboxykinase in plants exhibiting crassulacean acid metabolism.phosphoenolpyruvate carboxykinase has been found in significant activities in a number of plants exhibiting crassulacean acid metabolism. thirty-five species were surveyed for phosphoenolpyruvate carboxykinase, phosphoenolpyruvate carboxylase, ribulose diphosphate carboxylase, malic enzyme, and malate dehydrogenase (nad). plants which showed high activities of malic enzyme contained no detectable phosphoenolpyruvate carboxykinase, while plants with high activities of the latter enzyme contained ...197316658562
postillumination burst of carbon dioxide in crassalacean acid metabolism plants.immediately following exposure to light, a postillumination burst of co(2) has been detected in crassulacean acid metabolism plants. a detailed study with pineapple (ananas comosus) leaves indicates that the postillumination burst changes its amplitude and kinetics during the course of a day. in air, the postillumination burst in pineapple leaves generally is exhibited as two peaks. the postillumination burst is sensitive to atmospheric co(2) and o(2) concentrations as well as to the light inten ...197516659142
cellulase activity, endogenous abscisic acid, and ethylene in four citrus cultivars during maturation.at maturity, the fruit of two early maturing orange cultivars, hamlin and pineapple (citrus sinensis [l.] osbeck), contained more ethylene and abscisic acid than the late maturing valencia and lamb summer (c. sinensis [l.] osbeck) cultivars. ethylene (up to 95 nl/l in internal atmosphere) and abscisic (50 mug/kg dry weight flavedo) increased most rapidly in pineapple, leading to increased cellulase activity and loosening of the fruit. fruit of the two late maturing cultivars contained less than ...197516659390
carbon dioxide exchange and acidity levels in detached pineapple, ananas comosus (l.), merr., leaves during the day at various temperatures, oxygen and carbon dioxide concentrations.the effects of temperature, o(2), and co(2) on titratable acid content and on co(2) exchange were measured in detached pineapple (ananas comosus) leaves during the daily 15-hour light period. comparative measurements were made in air and in co(2)-free air. increasing the leaf temperature from 20 to 35 c decreased the total co(2) uptake in air and slightly increased the total co(2) released into co(2)-free air. between 25 and 35 c, the activation energy for daily acid loss was near 12 kcal mol(-1 ...197716659832
characterization of phosphoenolpyruvate carboxykinase from pineapple leaves ananas comosus (l.) merr.phosphoenolpyruvate carboxykinase has been partially purified from pineapple (ananas comosus [l.]) leaves. specific activities obtained show it to be a major activity in this tissue. above 15 c, the respective activation energies for decarboxylation and carboxylation are 13 and 12 kcal/mol. below 15 c, there are discontinuities in arrhenius plots with an associated large increase in activation energy. the adenine nucleotides are preferred to other nucleotides as substrates. the apparent km value ...197716659905
relationships between stomatal behavior and internal carbon dioxide concentration in crassulacean acid metabolism plants.measurements of internal gas phase co(2) concentration, stomatal resistance, and acid content were made in crassulacean acid metabolism plants growing under natural conditions. high co(2) concentrations, sometimes in excess of 2%, were observed during the day in a range of taxonomically widely separated plants (opuntia ficus-indica l., opuntia basilaris engelm. and bigel., agave desertii engelm., yucca schidigera roezl. ex ortiges, ananas comosus [l.] merr., aloe vera l., cattleya sp. and phalan ...197916660851
hydrolytic enzymes in the central vacuole of plant cells.the hydrolase content of vacuoles isolated from protoplasts of suspension-cultured tobacco cells, of tulip petals, and of pineapple leaves, and the sedimentation behavior of tobacco tonoplasts were studied. three precautions were found to be important for the analysis of vacuolar hydrolases and of the tonoplast. (a) purification of protoplasts in a ficoll gradient was necessary to remove cell debris which contained contaminating hydrolases adsorbed from the fungal cell-wall-degrading enzyme prep ...197916660869
the rapid isolation of vacuoles from leaves of crassulacean acid metabolism plants.a technique is presented for the isolation of vacuoles from sedum telephium l. leaves. leaf material is digested enzymically to produce protoplasts rapidly which are partially lysed by gentle osmotic shock and the inclusion of 5 millimolar ethyleneglycol-bis (beta-aminoethyl ether)n,n'-tetraacetic acid in the wash medium. vacuoles are isolated from the partially lysed protoplasts by brief centrifugation on a three-step ficoll-400 gradient consisting of 5, 10, and 15% (w/v) ficoll-400. a majority ...198016661440
diurnal variations in leaf fluorescence induction kinetics: variable fluorescence in crassulacean acid metabolism plants.the variable fluorescence of leaves from kalanchoë daigremontiana and pineapple, ananas comosus, both cam plants, was found to change over a 24-hour cycle and to exhibit high temperature-dependent maxima during the night period. the time course of the induced fluorescence was correlated with malic acid accumulation but not with other aspects of cam such as with the nature of the decarboxylation pathway or with stomatal movements. the variable fluorescences of sunflower (helianthus annuus l.) and ...198316663024
dark/light modulation of ribulose bisphosphate carboxylase activity in plants from different photosynthetic categories.ribulose bisphosphate carboxylase/oxygenase (rubpcase) from several plants had substantially greater activity in extracts from lightexposed leaves than dark leaves, even when the extracts were incubated in vitro with saturating hco(3) (-) and mg(2+) concentrations. this occurred in glycine max, lycopersicon esculentum, nicotiana tabacum, panicum bisulcatum, and p. hylaeicum (c(3)); p. maximum (c(4) phosphoenolpyruvate carboxykinase); p. milioides (c(3)/c(4)); and bromelia pinguin and ananas como ...198416663937
maintenance carbon cycle in crassulacean acid metabolism plant leaves : source and compartmentation of carbon for nocturnal malate synthesis.the reciprocal relationship between diurnal changes in organic acid and storage carbohydrate was examined in the leaves of three crassulacean acid metabolism plants. it was found that depletion of leaf hexoses at night was sufficient to account quantitatively for increase in malate in ananas comosus but not in sedum telephium or kalanchoë daigremontiana. fructose and to a lesser extent glucose underwent the largest changes. glucose levels in s. telephium leaves oscillated diurnally but were not ...198516664005
electrophoretic analysis of protoplast, vacuole, and tonoplast vesicle proteins in crassulacean acid metabolism plants.protoplasts and vacuoles were isolated and purified in large numbers from the cam plants ananas comosus (pineapple) and sedum telephium for protein characterization. vacuoles were further fractionated to yield a tonoplast vesicle preparation. polypeptides of protoplasts, vacuoles, and tonoplast vesicles were compared to whole leaf polypeptides from both plants by one-dimensional sodium dodecylsulfate-polyacrylamide gel electrophoresis. approximately 100 vacuole polypeptides could be resolved of ...198616665166
fructose 2,6-bisphosphate, carbohydrate partitioning, and crassulacean acid metabolism.fructose 2,6-bisphosphate (f 2,6-p(2)) was detected in the cam species, ananas comosus and bryophyllum tubiflorum, and in c(3)- and cam-mesembryanthemum crystallinum. in both mesembryanthemum tissues, f 2,6-p(2) was located outside the chloroplast. the levels of f 2,6-p(2), malate, starch, or soluble sugars were measured during various periods during the day-night cycle in the leaves of ananas, a species which stores carbohydrate in an extrachloroplastic compartment, and in bryophyllum, a specie ...198716665395
co(2) and o(2) exchanges in the cam plant ananas comosus (l.) merr: determination of total and malate-decorboxylation-dependent co(2)-assimilation rates; study of light o(2)-uptake.photosynthesis and light o(2)-uptake of the aerial portion of the cam plant ananas comosus (l.) merr. were studied by co(2) and o(2) gas exchange measurements. the amount of co(2) which was fixed during a complete day-night cycle was equal to the amount of total net o(2) evolved. this finding justifies the assumption that in each time interval of the light period, the difference between the rates of net o(2)-evolution and of net light atmospheric co(2)-uptake give the rates of malate-decarboxyla ...198916666547
soluble sugars as the carbohydrate reserve for cam in pineapple leaves : implications for the role of pyrophosphate:6-phosphofructokinase in glycolysis.neutral ethanol-soluble sugar pools serve as carbohydrate reserves for crassulacean acid metabolism (cam) in pineapple (ananas comosus (l.) merr.) leaves. levels of neutral soluble sugars and glucans fluctuated reciprocally with concentrations of malic acid. hexose loss from neutral soluble-sugar pools was sufficient to account for malic acid accumulation with about 95% of the required hexose accounted for by turnover of fructose and glucose pools. hexose loss from starch or starch plus lower mo ...198916666775
in vitro screening of six anthelmintic plant products against larval haemonchus contortus with a modified methyl-thiazolyl-tetrazolium reduction assay.because of the increasing anthelmintic resistance and the impact of conventional anthelmintics on the environment, it is important to look for alternative strategies against gastrointestinal nematodes. phytotherapy could be one of the major options to control these pathologies. extracts or ingredients of six different plant species were tested against exsheathed infective larvae of haemonchus contortus using a modified methyl-thiazolyl-tetrazolium (mtt) reduction assay. pyrantel tartrate was use ...200616725288
effect of ethanolic extracts of ananas comosus l. leaves on insulin sensitivity in rats and hepg2.ethanolic extracts of ananas comosus l. leaves (ac) enriched with phenols have hypoglycemic activity in diabetic rats. here, we investigated the effect of ac on insulin sensitivity in rats and hepg2. in high-fat diet-fed and low-dose streptozotozin-treated diabetic wistar rats subjected to challenge with exogenous human insulin, ac treatment at an oral dose of 0.40 g/kg could significantly improve sensitivity to exogenous insulin. after a sub-acute treatment, ac also could inhibit the developmen ...200616753349
quality changes and nutrient retention in fresh-cut versus whole fruits during storage.the influences of processing and storage on the quality indices and nutritional content of fresh-cut fruits were evaluated in comparison to whole fruits stored for the same duration but prepared on the day of sampling. fresh-cut pineapples, mangoes, cantaloupes, watermelons, strawberries, and kiwifruits and whole fruits were stored for up to 9 days in air at 5 degrees c. the postcutting life based on visual appearance was shorter than 6 days for fresh-cut kiwifruit and shorter than 9 days for fr ...200616756358
bioaffinity based oriented immobilization of stem bromelain.bromelain is a basic, 23.8 kda thiol proteinase obtained from stem of the pineapple plant (ananas comosus) and is unique in containing a single oligosaccharide chain attached to the polypeptide. this property allowed its affinity binding and favorable orientation on a sepharose support pre-coupled with the lectin, concanavalin a (con a). for comparison, bromelain was also immobilized by covalently coupling to the cnbr-activated sepharose. the preparation obtained was more resistant to thermal in ...200616786278
application of the broad-spectrum bacteriocin enterocin as-48 to inhibit bacillus coagulans in canned fruit and vegetable foods.the enterococcal bacteriocin (enterocin) as-48 is a broad-spectrum cyclic peptide. enterocin as-48 was tested against bacillus coagulans in three vegetable canned foods: tomato paste (ph 4.64), syrup from canned peaches (ph 3.97), and juice from canned pineapple (ph 3.65). when vegetative cells of b. coagulans cect (spanish type culture collection) 12 were inoculated in tomato paste supplemented with 6 microg/ml as-48 and stored at different temperatures, viable cell counts were reduced by appro ...200616831501
efficient production of transgenic citrus plants using isopentenyl transferase positive selection and removal of the marker gene by site-specific recombination.the presence of marker genes conferring antibiotic resistance in transgenic plants represents a serious obstacle for their public acceptance and future commercialization. in citrus, selection using the selectable marker gene nptii, that confers resistance to the antibiotic kanamycin, is in general very effective. an attractive alternative is offered by the mat system (multi-auto-transformation), which combines the ipt gene for positive selection with the recombinase system r/rs for removal of ma ...200716927091
moulds and yeasts in fruit salads and fruit juices.thirty-eight fruit salad samples including cantaloupe, citrus fruits, honeydew, pineapple, cut strawberries and mixed fruit salads, and 65 pasteurized fruit juice samples (apple, carrot, grapefruit, grape and orange juices, apple cider, and soy milk) were purchased from local supermarkets in the washington, dc area and tested for fungal contamination. the majority of fruit salad samples (97%) were contaminated with yeasts at levels ranging from <2.0 to 9.72 log10 of colony forming units per gram ...200616943069
on the proteolytic action of bromelin, the ferment of pineapple juice. 189316992096
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