Publications

characterization of variant gamma-glutamyl transpeptidase produced by pancreatocholangiocarcinoma cell lines in a protein-free, chemically defined medium.	four pancreatocholangiocarcinoma cell lines (hpc-y1, hpc-yt, mia paca-2, and hchol-y1) were established to propagate in a protein-free, chemically defined medium. high gamma-glutamyl transpeptidase (ggtp) activities were showed in their spent media (designated as the secreted (ggtp). their ggtp activities in the spent media were 125, 85, 110, and 153 iu/l/mg of lyophilized spent media, whereas ggtp activities extracted from their cancer cell lines with bromelain were 105, 37, 86, and 112 iu/l/1 ...	1989	2569734
correlation between carbohydrate structures on the envelope glycoprotein gp120 of hiv-1 and hiv-2 and syncytium inhibition with lectins.	the binding of 13 different lectins to gp120 partially purified from two hiv-1 isolates and one hiv-2 isolate was studied by in situ staining on electrophoretically separated and electroblotted hiv antigens. the lectins concanavalin a, wheat germ agglutinin, lens culinaris agglutinin, vicia faba agglutinin, pisum sativum agglutinin and phytohaem(erythro)agglutinin bound to gp120 of all three isolates. the carbohydrate of gp120 recognized by lectins was thus arranged in at least four types of gly ...	1989	2574581
glycosylated human prolactin.	a glycosylated form of human prl (g-hprl) was isolated from pituitary glands. the glycoprotein was separated from the major form of prl on columns of lentil lectin-sepharose 4b. the major form of prl did not bind to the lentil lectin, whereas the glycosylated modification did and could be eluted with methyl-alpha-d-mannopyranoside. by gel electrophoresis in sodium dodecyl sulfate, a mol wt of 25,000 was estimated for the glycosylated prl. the mol wt of hprl is 23,000. in a ria for hprl, the glyc ...	1985	2578035
natural killer (nk) cell activating factor released from murine thymocytes stimulated with an anti-tumor streptococcal preparation, ok-432.	natural killer (nk) activity of mouse splenocytes was significantly augmented when the splenocytes were incubated for 3 to 4 hr with culture supernatants of mouse thymocytes stimulated by ok-432, an antitumor preparation from the streptococcus pyogenes su-strain. antiviral activity was also detected in the culture supernatants, but il 2 activity was not. when the culture supernatants of thymocytes stimulated by ok-432 were fractionated on a column of blue sepharose cl-6b, nk enhancing activity a ...	1985	2578148
a glycoprotein of molecular weight 85,000 on human cells of b-lineage: detection with a family of monoclonal antibodies.	immunization of balb/c mice with glycoproteins purified from a detergent extract of human chronic lymphocytic leukemia (cll) cells by affinity to lens culinaris lectin led to the production of several monoclonal antibodies with similar reactivity. one of the antibodies, 50b4, was purified and the corresponding antigen was isolated from a b-lymphoblastoid cell line extract by affinity chromatography to the 50b4-igg immunoadsorbent. co-purification of the antigenic activities associated with five ...	1985	2579328
ultrastructure and lectin histochemistry of equine cutaneous histiolymphocytic lymphosarcomas.	tissues from subcutaneous lymphosarcomas and regional lymph nodes were examined by light and electron microscopy and by lectin histochemistry. tumors were composed of two major cell types: small lymphocytes with few organelles and pleomorphic histiocytic cells with undulant surfaces, large numbers of cytoplasmic vacuoles, and many mitochondria with large crystalline inclusions. a large gram-positive coryneform bacterium was isolated from tumor nodules but was not identified morphologically in tu ...	1989	2588437
developmental changes in proteins and glycoproteins revealed by direct radio-iodination of viable taenia saginata larvae.	direct surface 125i radio-isotope labelling techniques and sds-page analysis were used to compare the proteins and lentil-lectin adherent glycoproteins of the bovine stage of viable taenia saginata larvae at three points in their development, the invasive oncospheres, immature (4-week-old) and mature (12 to 16-week-old) cysticerci. some proteins and glycoproteins were present on all three of the ages of the parasite examined but there were also distinct age-specific proteins and glycoproteins de ...	1989	2594418
a detailed analysis of the potential of water-soluble classical class i mhc molecules for the suppression of kidney allograft rejection and in vitro cytotoxic t cell responses.	water-soluble classical (rt1-a) class i mhc molecules were purified from aqueous extracts of da strain liver. following monoclonal antibody affinity, lentil lectin affinity, and gel filtration chromatography, 600 micrograms of soluble rt1-a class i molecules with antigen activity equivalent to 1.3 x 10(11) nucleated da spleen cells (greater than 500 da spleens) was obtained. both pvg and lew strain recipients of da kidney allografts were pretreated with intravenous injections of the da soluble c ...	1989	2595764
a novel metalloproteinase associated with brain myelin membranes. isolation and characterization.	brain myelin membrane preparations contain a metalloproteinase activity which degrades myelin basic protein (mbp). the activity was associated with lentil lectin-binding glycoproteins solubilized from myelin and could be detected in the presence of the detergent 3-[(3-cholamidopropyl)dimethylammonio]-1-propane-sulfonate (chaps). the metalloproteinase represented about 5% of this glycoprotein fraction and was isolated from it by chromatography on deae-sephacel, cm-sepharose, and superose 6. the p ...	1989	2600084
autolysis of protein bodies in germinating lentil seeds.	protein bodies isolated from lentil (lens culinaris, medik) cotyledons exhibit autolytic activity which increases during seed germination. such autolytic capacity is active across a broad ph range and shows a maximum at ph 6.5. excision of the embryonic axis reduces autolytic capacity and application during incubation of the seeds without axis of both 6-benzylaminopurine and kinetin is able to replace it. on the other hand, the proteolytic activity in the protein body membrane, is located toward ...	1989	2616873
cycle- and function-related changes in lectin binding to human endometrium: a histochemical study with pronase treatment.	forty-eight endometrial biopsy specimens were obtained during a normal menstrual cycle, during pregnancy and from patients with dysfunctional bleeding. the specimens were examined for peanut (pna), soybean (sba), vicia villosa (vva), phytohem- (pha), lens culinaris (lca) and concanavalin (succ. con a) agglutinin binding. the study was performed on paraffin sections using the pronase digestion and either the peroxidase-antiperoxidase or the avidin-biotin-peroxidase method. cycle-related changes o ...	1989	2619335
developmental changes in the glycosylation and binding properties of human fibronectins. characterization of the glycan structures and ligand binding of human fibronectins from adult plasma, cord blood and amniotic fluid.	fibronectins from human adult plasma, fetal plasma and from amniotic fluid obtained during early and late gestation were compared with respect to (i) their reactivity with lectins, (ii) their binding to the physiological ligands gelatin and heparin, and (iii) the role of the carbohydrate residues in the binding to these two ligands. the two fibronectin isoforms displayed distinct developmental differences in both glycosylation and binding properties: (i) proportions of tri/tetraantennary complex ...	1989	2619923
purification and sequencing of glycosylation variants of bsf-1, as a maf, from the el-4 leukaemia cell line.	macrophage activation activity was characterized from a pma-induced subclone of the murine el-4 leukaemic cell line. the maf was purified from the cell line culture supernatant by concentration, cm-sepharose and lentil lectin sepharose chromatography, aca 54 gel filtration, mono q fplc and reverse phase hplc. four protein bands of different abundance were observed on sds-page with molecular weights of 17,500 to 21,000 da. three of the four proteins were sequenced from the n-terminal and shared h ...	2009	2646299
characterization of octyl glucoside-solubilized cell membrane receptors for binding measles virus.	optimum conditions were determined for solubilizing cell membrane receptors for binding measles virus (mv). evidence for specific receptors was shown from the saturability of mv binding to intact vero cells or african green monkey erythrocytes (mrbcs). receptors, solubilized from vero cells and mrbc with 1.5% octyl glucoside, inhibited mv attachment, infectivity, and hemagglutination activities. extracts from chicken erythrocytes, which did not bind mv, were inactive in all assays. mv binding ac ...	1989	2672566
specific detection of circulating surface/secreted glycoproteins of viable cysticerci in taenia saginata cysticercosis.	a mouse monoclonal antibody (moab), reactive with a repetitive carbohydrate epitope on lentil-lectin adherent glycoproteins present on the surface and in the secretions of taenia saginata cysticerci, was used in the construction of a diagnostic elisa assay to detect these glycoproteins in the serum of t. saginata infected cattle. the moab was used as the trapping layer and subsequently bound glycoprotein was revealed using the same moab and biotinylated peroxidase-streptavidin complex as the dev ...	1989	2674862
purification and properties of a protease from developing porcine dental enamel.	a protease of molecular weight 29,000 was isolated and purified using ammonium sulphate precipitation, lentil lectin-sepharose affinity chromatography and deae-5pw ion-exchange chromatography. the protease had an unusual amino acid composition including 5% serine, 6% proline and 20% tyrosine. it was a glycoprotein containing 12-15% carbohydrate by weight. activity was optimal at 40-45 degrees c using [3h]-acetyl casein substrate and at 40-55 degrees c using [3h]-acetyl enamel protein substrate. ...	1989	2684109
mitogenic lectins bind to the antigen receptor on human lymphocytes.	the specificity of interactions between mitogenic and non-mitogenic lectins and disulfide-linked cell surface receptors on human lymphocytes was explored. lysates (nonidet-p40) of surface-radioiodinated tonsil lymphocytes and t lymphoblastoid cells (hpb-all) were absorbed with lectin-agarose derivatives (or bovine serum albumin, bsa-agarose) or immunoprecipitated with appropriate monoclonal antibodies (mab). lectin eluates and solubilized immunoprecipitates were analyzed by two-dimensional (nonr ...	1989	2703017
clonal analysis of the glycosylation of immunoglobulin g secreted by murine hybridomas.	a panel of 10 hybridomas was assembled to assess the influence of various genetic and biological factors upon glycosylation of secreted monoclonal igg. after exhaustive pronase digestion of igg, glycopeptides were characterized chromatographically by apparent size, charge, and concanavalin a (con a)-sepharose and lens culinaris (lch)-agarose affinity. six glycosylation phenotypes were found to be common among all clones studied. despite this phenotypic heterogeneity in glycosylation of igg, cons ...	1989	2713370
differential response of adherent and unanchored melanoma cells to bromodeoxyuridine evidenced by specific lectin-binding protein changes.	the possible differential response of adherent and nonadherent cells of the same tumor type to pyrimidine analogues has been investigated. we show that bromodeoxyuridine (budr) increases interactions of attached cells with their substrate without markedly affecting the cell adhesion properties of the same cells when these are not anchored. however, evidence for an adhesion-independent response of both cell types to budr has been obtained with lectin binding assays using 125i-labelled lens culina ...	1989	2714888
lectin histochemistry of mammalian brunner's glands.	lectin histochemical study was performed on twenty-eight specimens of formalin-fixed paraffin embedded tissues of proximal duodenum from human, cat, dog and rhesus (macaque) monkey to demonstrate the pattern of carbohydrate residues in submucosal glands of brunner as compared to that of the duodenal absorptive and goblet cells. ten different biotinylated lectins were used as probes, and avidin-biotin-peroxidase (abc) or avidin-gold-silver (ags) complexes were used as "visualants". brunner's glan ...	1989	2715049
wheat germ agglutinin, concanavalin a, and lens culinalis agglutinin block the inhibitory effect of nerve growth factor on cell-free phosphorylation of nsp100 in pc12h cells.	it has been shown that in pc12 and its subclone pc12h treatment of the cells with nerve growth factor (ngf) induces a selective decrease in the incorporation of radioactive phosphate into a 100,000-dalton protein, designated in an earlier study as nsp100, in the subsequent phosphorylation of soluble extracts from cells with (gamma-32p)atp. in the present study, we show that plant lectins, wheat germ agglutinin (wga), concanavalin a (con a), and lens culinaris agglutinin (lca), inhibit the action ...	1989	2720800
macrophage differentiation inducing factor from human monocytic cells is equivalent to murine leukemia inhibitory factor.	human macrophage differentiation inducing factor (dif) can induce differentiation of human myeloid leukemic cells into macrophage-like cells in vitro. a procedure is described for purification of dif from serum-free human monocytic leukemia thp-1 cell-conditioned medium. the procedure included concentration of a conditioned medium by ultrafiltration, lentil lectin-sepharose affinity chromatography, and fast protein liquid chromatography using mono s and mono q. dif-a of pi 9.0 and dif-b of pi 8. ...	1989	2722807
characterization and solubilization of cyclo(his-pro) binding from rat liver membranes.	we have found cyclo(his-pro) binding in rat liver plasma membranes. this study focused on the characterization of solubilized binding for cyclo(his-pro) in rat liver membranes. the cyclo(his-pro) binding of liver membranes was solubilized by digitonin and octyl-glucopyranoside. the efficiency of solubilization with digitonin was greater. however, cyclo(his-pro) binding was not solubilized by triton x-100, chaps, or lubrol. digitonin-solubilized membranes showed cyclo(his-pro) binding with a high ...	1989	2726776
[identification of a human sperm antigen to infertile women's serum and follicular fluid with high sperm immobilizing activity].	in our previous paper, we reported a sperm coating antigen with a molecular weight of approximately 12,000 daltons from sperm membrane fraction by using rabbit anti-seminal plasma antiserum. in this paper, we isolated a human sperm antigen with the same sperm preparation by using sera of infertile women with sperm immobilizing antibody. the isolated antigen showed the following characteristics: (1) it is a glycoprotein with an affinity with lentil lectin and its molecular weight is approximately ...	1989	2746071
glycosylation reduces the bioactivity of calcitonin.	the biological significance of peptide hormone glycosylation is uncertain. to examine the effect of asn-linked glycosylation on calcitonin's bioactivity we purified glycosylated calcitonin from a transplantable rat medullary thyroid carcinoma. glycosylated calcitonin constituted 2.3% of the total extracted immunoreactive calcitonin. the structure of this peptide differed from nonglycosylated calcitonin only by the oligosaccharide modification of asparagine 3. affinity of glycosylated calcitonin ...	1989	2748417
evidence of altered structural and secretory glycoconjugates in the jejunal mucosa of patients with gluten sensitive enteropathy and subtotal villous atrophy.	the pattern of lectin histochemistry in formalin fixed, paraffin embedded normal jejunal and subtotal villous atrophy specimens from patients with gluten sensitive enteropathy were compared. there was no significant difference in the binding pattern of five lectins (arachis hypogaea, canavalia ensiformis, lens culinaris, phaseolus vulgaris and triticum vulgaris) between normal and abnormal specimens. there were significant changes in the binding pattern of three lectins (dolichos biflorus, ulex ...	1989	2753405
identification of protein-bound oligosaccharides on the surface of growth cones that bind to muscle cells.	in the accompanying paper (gabel, den, and ambron, in press) it was shown that eight populations of glycopeptides are synthesized by single neurons of aplysia californica. to see which glycopeptides might mediate interactions with target cells, we first identified glycopeptides that are transported selectively to synapses and growth cones. the giant neuron r2 was injected intrasomatically with 3h-glucosamine. twenty-four hours later, 3h-glycopeptides in the axon and cell body were isolated and r ...	2009	2760609
glycoconjugates in exfoliation syndrome. a lectin histochemical study of the ciliary body and lens.	the binding of ten biotin-coupled lectins with different carbohydrate specificities to exfoliative material and neighbouring tissues was studied in 16 formalin-fixed and paraffin-embedded human eyes. eight of the eyes were exfoliation positive while the rest were exfoliation negative. exfoliative material reacted intensely with lens culinaris (lca), canavalia ensiformis (cona) and ricinus communis (rca-i) agglutinins. positive reaction was also seen with wheat germ (wga), peanut (pna) and soybea ...	1989	2763817
the interaction of substrate and taurine modulates the outgrowth from regenerating goldfish retinal explants.	retinal growth from explants attached to different substrates was evaluated in the absence and the presence of various concentrations of taurine. fiber thickness was higher in poly-l-lysine, collagen and laminin than in the other substrates tested. explants on wheatgerm, soybean, and red kidney bean lectins did not show emission of neurites at 5 days in culture; growth on laminin was very poor at this period of time. by day 10 nerve growth index was possible to evaluate on soybean and laminin. o ...	2009	2773672
glycoconjugates of the intestinal epithelium of the domestic fowl (gallus domesticus): a lectin histochemistry study.	a lectin histochemical study was performed on formalin-fixed paraffin-embedded tissues of duodenum, jejunum, ileum, caecum and colon from six fasted and six non-fasted 8-week-old chickens (gallus domesticus). the purpose of this study was to identify in situ the pattern of carbohydrate residues present on the luminal surface of the intestinal epithelium. ten biotinylated lectins with different sugar specificities were used as probes, and avidin-biotin-peroxidase complex (abc) was used as a 'visu ...	1989	2777634
fractionation of plasmic fibrin(ogen) digests by lectin affinity chromatography.	a method for the fractionation of plasmic digests of both fibrinogen and fibrin was developed by taking advantage of the different chromatographic behaviour of fibrinogen and its fragments on immobilized concanavalin a and lens culinaris agglutinin. columns with different lectin concentration but with the same total lectin content were tested. fragment e was retained on all the concanavalin a-sepharose preparations while fragment d was mostly eluted in the unbound fraction. however, the binding ...	1989	2781526
induction of ige synthesis in anti-igm-activated nonatopic human b cells by recombinant interleukin-3.	the signals required to induce purified normal human b cell subpopulations into ige production were studied. pokeweed mitogen (pwm)-stimulated t cell supernatant induced ige synthesis in low-density but not high-density percoll-gradient-separated resting b cells. the pwm supernatant also enhanced (greater than 2-fold) ige synthesis by anti-igm (but not staphylococcus a cowan i)-activated high-density b cells (but not low-density b cells) and had affinity for lentil lectin. subsequent studies wer ...	1989	2786506
[study of the carbohydrate components of the surface of s 1300 neuroblastoma cells with the use of cytochemical and lectin agglutination methods].	the carbohydrate determinants of the cell surface of neuroblastoma c 1300 clone n 18 have been investigated by light microscopy with a panel of 11 lectins as cytochemical reagents. the expression of n- and o-glycanes with a predominance of 3-4 antigenic n-glycosyl chains was revealed. the characteristic feature of neuroblastoma cells is an abundance of an antigen a surface determinant. a strong correlation is shown between the intensity of lectin binding and index of cell agglutination. after th ...	1989	2791953
concanavalin-a, lentil, and ricin lectin affinity binding characteristics of human thyrotropin: differences in the sialylation of thyrotropin in sera of euthyroid, primary, and central hypothyroid patients.	tsh from human serum was separated into classes by serial lectin affinity chromatography using concanavalin-a (cona), lentil, and ricin lectins. tsh from 10 euthyroid subjects, 40 patients with primary hypothyroidism, and 1 patient with central hypothyroidism was studied. the patterns of cona and lentil affinity binding were similar for diverse patients; forms of tsh that bound firmly to cona also tended to bind firmly to lentil. differences in tsh-ricin binding suggested that there were differe ...	1989	2793998
amine oxidase from lathyrus cicera and phaseolus vulgaris: purification and properties.	cu-amine oxidases (amine oxygen oxidoreductase deaminating, copper containing e.c. 1.4.3.6.) are found in all forms of life (1). they catalyze the following general reaction: r-ch2-nh2 + o2 + h2o----r-cho + nh3 + h2o2. cu-amine oxidases (cu-aos) have been extracted from different leguminosae: pisum sativum (2-3), lathyrus sativus (4), lens esculenta (5), vicia faba (6), cicer arietinum (7), glycine max (8) but not from phaseolus vulgaris. palavan and galston (9), in a study of polyamine biosynth ...	1989	2798365
immunochemical characterization of the suppressor factor from early human decidual cells.	an immunosuppressive factor was obtained from culture supernatants of early human decidual cells. the suppressor factor was concentrated by gel filtration in a fraction with a molecular weight between 43,000 and 67,000 daltons. it was further purified by biochemical methods. four peaks were obtained in the fraction with molecular weight between 43,000 and 67,000 daltons by anion exchange chromatography. only the second peak had immunosuppressive activity in mlr. lentil-lectin affinity chromatogr ...	1989	2799918
analysis of wheat germ agglutinin (wga), phaseolus vulgaris leucoagglutinin (pha-l), and lens culinaris agglutinin (lca) binding to isolated rat neocortical membrane glycoproteins and to brain tissue sections.	different lectin transport directions--either anterograde or retrograde in tracing neuronal pathways--have been attributed to different sugar specificities of the lectins. to test this hypothesis, transmembrane neocortical glycoproteins were isolated to analyze their lectin-binding properties. the lectins tested exhibited a broad binding pattern to these glycoproteins. arguing against the above-mentioned hypothesis, sugar residues of transmembranal glycoproteins probably are not exclusively resp ...	1989	2819434
expression of human extracellular superoxide dismutase in chinese hamster ovary cells and characterization of the product.	a complementary dna clone from human placenta, encoding human extracellular superoxide dismutase (ec-sod; superoxide:superoxide oxidoreductase, ec 1.15.1.1), has recently been isolated and characterized. an expression plasmid, based on the ec-sod complementary dna, was transfected into chinese hamster ovary cells (cho-k1). the transfected cells secreted human ec-sod to the culture medium. the secreted recombinant (r) ec-sod was isolated in high yield with a three-step procedure beginning with im ...	1987	2821539
the reactivity of alpha-1-antitrypsin with lens culinaris agglutinin and its usefulness in the diagnosis of neoplastic diseases of the liver.	the reactivity of alpha-1-antitrypsin (aat) with lens culinaris agglutinin (lca) was studied by crossed immuno-affinity electrophoresis of the sera of 246 subjects from 6 groups (acute virus hepatitis, chronic hepatitis, liver cirrhosis, hepatocellular carcinoma (hcc), carcinoma metastatic to the liver and normal controls). two species of aat (lca-reactive and -nonreactive species) were detected on crossed immuno-affinity electrophoresis in a gel containing lca. the percentages of lca-reactive s ...	2009	2822074
[intramolecular mobility of human major histocompatibility complex protein. a spin-label study].	membranes of human splenocytes were hydrolyzed by papain and extracellular portions of class i and class ii hla antigen molecules were isolated by monoclonal antibodies fixed on sepharose 4b. the isolated proteins were spin-labeled by tempo-dichlorotriazine and the values of rotational correlation times (tau) of labeled proteins were found using dependencies of esr spectra parameters vs viscosity at constant temperature. the tau-values were equal to 8 ns for class i molecules and 14 ns for class ...	1987	2824988
evidence that mannosyl residues are involved in human immunodeficiency virus type 1 (hiv-1) pathogenesis.	the human immunodeficiency virus (hiv), the causative agent of aids, is thought to bind to t4+ (cd4+) target cells through the heavily glycosylated gp120 envelope glycoprotein. plant lectins bind glycoproteins through noncovalent interaction with specific hexose residues; therefore, lectins were evaluated for their ability to inactivate hiv in vitro. the mannose-specific lectins concanavalin-a and succinyl concanavalin-a completely inactivated hiv while lentil lectin, wheat germ agglutinin, and ...	1987	2829950
characteristics of the minor group receptor of human rhinoviruses.	the receptor for the minor group of human rhinoviruses was solubilized from hela cell membranes with various detergents. virus binding activity was determined in a filter binding assay using 35s-labeled human rhinovirus 2 (hrv2) as a probe. the receptor protein was enriched on lens culinaris lectin columns and the active fractions were further purified by gel permeation and anion exchange chromatography. the receptor has an apparent molecular weight of 450 kda in the presence of detergent. the b ...	1988	2831654
purification of envelope glycoproteins of human t cell lymphotropic virus type i (htlv-i) by affinity chromatography.	the external envelope glycoprotein (gp46) and transmembrane glycoprotein (gp21) of human t-cell lymphotropic virus type i (htlv-i) were isolated from lysates of htlv-i-infected hut-102 cells by affinity chromatography. fifty ml aliquots of packed hut-102 cells were extracted with 1% triton x-100, and lysates were treated sequentially with an affinity column containing igg from an htlv-i+ human subject followed by chromatography of the bound fraction over a lentil lectin column. the identity of t ...	1987	2832431
a quantitative analysis of lectin binding to adult rat hepatocyte cell surfaces.	a quantitative evaluation of lectin binding to adult rat hepatocyte cell surfaces was done using cells isolated by two different collagenase perfusion methodologies and cultured as monolayers with two different tissue culture media formulations (protocol i vs. protocol ii). the presence of alpha-d-mannosyl and alpha-d-glucosyl groups was detected by the binding of concanavalin a (con a), lens culinaris agglutinin (lca), and pisum sativum agglutinin (psa) to freshly isolated cells. furthermore, b ...	1988	2836355
the use of lectin affinity columns for selection of precursor or fully glycosylated forms of glycoprotein gd1 of herpes simplex virus type 1.	several lectins were examined for their ability to bind to the glycoprotein gd1 polypeptide from vero cells infected by herpes simplex virus type 1 (hvs-1), strain kos. at least four distinct forms of gd1 (1, 2, 3 and 4), ranging in size from 59k to 52k, were resolved by sds-10% polyacrylamide gel electrophoresis. wheat germ agglutinin (wga) did not bind to any of these forms, suggesting that if any sialic residues are present in the carbohydrate moieties of gd1, they are not available for bindi ...	1988	2840449
inhibition of lymphocyte 5'-nucleotidase by lectins: effects of lectin specificity and cross-linking ability.	5'-nucleotidase, an integral glycoprotein enzyme of the lymphocyte plasma membrane, is inhibited cooperatively by the lectin concanavalin a. because divalent succinyl-concanavalin a is a poor enzyme inhibitor, both binding and lectin-induced cross-linking of 5'-nucleotidase may be necessary for inhibition. succinyl-concanavalin a does not compete with concanavalin a for binding to the enzyme; however, maleyl-concanavalin a, another poor inhibitor, competes effectively with the parent lectin. thu ...	2010	2846011
hepatocyte cell surface polarity as demonstrated by lectin binding.	we performed an investigation at the ultrastructural level of the differential distribution of lectin-binding sites among sinusoidal, lateral, and bile canalicular domains of adult rat hepatocytes. lectin binding to hepatocyte glycocalices was studied in situ or after cellular dissociation by enzymatic (collagenase), chemical (edta), and mechanical methods, as well as during cell culture. using thirteen biotinylated lectins and an avidin-biotin-peroxidase complex (abc), we have identified lectin ...	1988	2848070
purification and properties of human placental dipeptidyl peptidase iv.	dipeptidyl peptidase iv (ec 3.4.14.5; gly-pro-p-nitroanilidase) was purified 1840-fold from human placenta and characterized. the enzyme was solubilized from membrane fractions with triton x-100 and subjected to zinc acetate fractionation, hydroxylapatite chromatography, sephacryl s-300 chromatography and then affinity chromatographies with lentil lectin-sepharose 4b and gly-pro-nh-(ch2)6-nh-sepharose 4b. dipeptidyl peptidase iv was completely separated from leucine aminopeptidase by the final a ...	1985	2860191
characterization of gamma-glutamyltranspeptidase from human exocrine pancreatic carcinomas.	two different types of gamma-glutamyltranspeptidase (gamma-gtp) have been found in normal human pancreas following bromelain treatment. on the other hand, three human pancreatic ductal cell carcinomas have only a single type of gamma-gtp upon analysis with polyacrylamide gel electrophoresis, anion-exchange column chromatography and isoelectric focusing. carcinoma gamma-gtps were almost identical to one of the two types of normal pancreatic gamma-gtps. the gamma-gtp from pancreatic carcinomas bou ...	1985	2863014
isolation and characterization of thy 1 homologue from human thymus.	a 40 000 m.w. glycoprotein was isolated from human thymus. this molecule binds lentil lectin, reacts with an antiserum made against the p25 antigen (the human thy 1 homologue) and possesses almost identical amino acid composition as the p25 antigen and its 40 000 m.w. dimer.	1985	2864756
lentil lectin binding properties of the leucocyte common antigen from rat dividing and non-dividing thymocytes.	the surface glycoproteins of rat large, mainly dividing, thymocytes and their small, non-dividing, progeny have been examined by labelling with 3h-sodium borohydride. a prominent glycoprotein component of apparent mol. wt. 150 k was labelled on both the cell types and by immunoprecipitation with a specific monoclonal antibody, was demonstrated to contain the leucocyte common antigen (lca). the binding of this glycoprotein to lens culinaris lectin (lch) has been studied. preliminary results obtai ...	1985	2867624
partial characterization of chicken thy-1 glycoprotein by monoclonal antibodies.	a panel of monoclonal antibodies has been raised to thy-1 purified from chicken brain. they were produced by immunization of balb/c mice with a thy-1-enriched (sephacryl b) fraction of a lentil lectin-positive fraction from solubilized brain membrane proteins. antibody-secreting clones were found to be specific for thy-1 by enzyme-linked immunosorbent assay (elisa) and western transfer and immunoblotting. four of the monoclonal antibodies were of the igg2b isotype, one was igg1, and one was igm. ...	1986	2877095
purification and characterization of human placental microsomal aminopeptidase: immunological difference between placental microsomal aminopeptidase and pregnancy serum cystyl-aminopeptidase.	human placental microsomal aminopeptidase (microsomal pap) was purified 3,880-fold from human placenta and characterized. the enzyme was solubilized from membrane fractions with triton x-100 and also trypsin digestion, and subjected to zinc sulfate fractionation, chromatographies with de-52, hydroxylapatite, sephacryl s-300 and lentil lectin-sepharose 4b, and finally affinity chromatography with bestatin-sepharose 4b. microsomal pap was separated from aminopeptidase a (aap) by affinity chromatog ...	1986	2877870
characterization of covalently cross-linked pancreatic somatostatin receptors.	the receptor for somatostatin present in rat pancreatic plasma membranes was characterized by affinity labeling with [125i-tyr11]somatostatin utilizing three different heterobifunctional cross-linking agents: n-5-azido-2-nitrobenzoyloxy-succinimide, n-succinimidyl 6-(4-azido 2'-nitrophenylamine)hexanoate, and n-hydroxysuccinimidyl 4-azido-benzoate. analysis by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and autoradiography revealed a broad band of mr = 92,000 when any of the three ...	1986	2877990
lectin-binding characteristics of wuchereria bancrofti microfilariae.	the binding of 10 different lectins to the surface of microfilariae of wuchereria bancrofti has been investigated. wheat germ agglutinin (wga) and helix pomatia lectin (hpa) bound specifically to the sheathed microfilariae indicating the presence of n-acetyl-d-glucosamine and n-acetyl-d-galactosamine respectively on the surface. exsheathed microfilariae did not react with any of the lectins. treatment of sheathed microfilariae with proteases resulted in increased binding of wga and hpa. such tre ...	1987	2884837
tissue-specific n-glycosylation, site-specific oligosaccharide patterns and lentil lectin recognition of rat thy-1.	to examine the extent to which protein structure and tissue-type influence glycosylation, we have determined the oligosaccharide structures at each of the three glycosylation sites (asn-23, 74 and 98) of the cell surface glycoprotein thy-1 isolated from rat brain and thymus. the results show that there is tissue-specificity of glycosylation and that superimposed on this is a significant degree of site-specificity. on the basis of the site distribution of oligosaccharides, we find that no thy-1 m ...	1987	2886334
squid glycoproteins with structural similarities to thy-1 and ly-6 antigens.	in an attempt to identify invertebrate homologs of thy-1 antigen, the optic and central nervous tissue of squid was solubilized in deoxycholate and fractionated by lentil lectin affinity chromatography and gel filtration to yield small abundant glycoproteins. material with biochemical similarities to thy-1 was found and shown to consist of two glycoproteins that were ultimately purified using monoclonal antibody affinity columns. both glycoproteins were sequenced to yield sequences of 84 residue ...	1988	2894353
an enzyme-linked immunoelectrotransfer blot assay and glycoprotein antigens for diagnosing human cysticercosis (taenia solium).	an enzyme-linked immunoelectrotransfer blot (eitb) assay was developed for immunodiagnosing human cysticercosis. the assay uses lentil-lectin, affinity-purified glycoprotein antigens. a battery of 532 serum and 46 cerebrospinal fluid (csf) samples (148 cases of parasitologically confirmed cysticercosis, 54 healthy controls, and 18 types of heterologous infections [376 cases]) were used to ascertain the assay's efficacy. all but three of the samples from cases of confirmed cysticercosis were posi ...	1989	2909643
the role of carbohydrates in the igg binding and suppressive activities of shed human fc receptors.	igg fc receptor (fc gamma r) shed from human peripheral mononuclear cells and purified by igg affinity chromatography bound to cona, pisum sativum, ulex europeus and peanut lectins. the soluble fc gamma r was not absorbed by wheat germ, soyabean, lentil, sophora japonica or helix pomotia lectins. out of 14 different sugars, none interfered with the interaction between igg fc and membrane fc gamma r. these results show that shed fc gamma r had glucosyl, mannosyl, fucosyl and galactosyl groups, bu ...	1986	2944472
lectin histochemistry of plaques and tangles in alzheimer's disease.	biotinyl derivatives of several lectins and avidin-horseradish peroxidase were used to study the localization of glycoconjugates in amyloid plaques and in neuritic tangles in brains of patients with alzheimer's disease (ad), downs syndrome (ds) and gerstmann-sträussler syndrome (gss). the lectins tested recognize the following residues: beta-d-galactosyl [ricinus communis agglutinin 120, (rca-1) and peanut agglutinin, (pna)]; alpha-d-galactosyl [griffonia simplicifolia agglutinin (gsa)]; alpha-d ...	1987	2955631
effect of lectin-binding to fibrinogen d and e domains on coagulation and fibrinolysis.	the effect on fibrinogen coagulation and fibrinolysis of the mannose-specific lectins concanavalin a, its acetyl derivative and lens culinaris agglutinin was studied. concanavalin a and acetyl-concanavalin a, which bind to the four carbohydrate chains of fibrinogen, and l. culinaris agglutinin, which only binds to the carbohydrate present in fibrinogen d domains, has the same effect on the coagulation rate: an inhibition at low lectin concentrations and an increase at high concentrations. on the ...	2010	2958091
purification of the beta subunit of the fibronectin receptor.	in this work we describe a method for purification of the beta subunit of the mouse fibronectin receptor (gp135). cellular glycoproteins were isolated from a detergent extract of sr-balb tumor cell membranes by two steps of affinity chromatography on lentil lectin-sepharose and wheat-germ-agglutinin--agarose. this material was subsequently bound to an affi gel 102 column and eluted by increasing salt concentration. most of the gp135 was eluted at 80 mm sodium chloride together with a few other c ...	1988	2965011
purification, characterization, and immunochemical studies of beta-n-acetyl-d-hexosaminidase from the parasitic nematode trichinella spiralis.	the exoglycosidase, beta-n-acetyl-d-hexosaminidase was purified 600-fold from the muscle-stage larvae (l1) of trichinella spiralis. on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page), the purified enzyme-active fraction contained 4 polypeptides with apparent molecular weights of 100,000, 68,000, 58,000 and 54,000. the beta-n-acetyl-d-hexosaminidase corresponds to the mr 100,000 polypeptide as demonstrated by sds-page analysis of the enzyme-stained region isolated from a non- ...	1988	2972930
galactose-rich glycoproteins are on the cell surface of herpes virus-infected cells. 1. surface labeling and serial lectin binding studies of asn-linked oligosaccharides of glycoprotein gc.	cell-surface glycoproteins of mock-infected and herpes simplex virus type 1 (hsv-1)-infected bhk-21 and hep-2 cells were radiolabeled by incubation with galactose oxidase followed by reduction with nab3h4. the incorporation of radiolabel into glycoconjugates in both bhk-21 and hep-2 cells was increased several fold following infection with hsv, showing an increase in surface-exposed gal residues in the infected cells. this was further confirmed by an increase in binding of cell-surface-labeled g ...	1985	2982317
redistribution of membrane-bound and cytosolic action in rabbit polymorphonuclear leucocytes during phagocytosis.	in the analysis of highly purified surface membrane from both resting and phagocytosing neutrophils an increase in the surface membrane associated actin has been demonstrated. this change at the cell periphery is associated with a coincident increase in the f-actin content of the cells following stimulation of the cells by exposure to opsonized oil red o droplets. the actin which is newly associated with the surface membrane of the phagocytosing cells was more susceptible to removal by detergent ...	1985	2983683
isolation of canine neutrophil plasma membranes.	this paper describes the isolation of plasma membrane vesicles formed by nitrogen cavitation of canine neutrophils. plasma membranes from disrupted cells were separated from other membranes and organelles by percoll-density gradient centrifugation. transmission electron microscopic examination of membrane preparations chromatographed on either sephacryl s-1000 or sepharose 4b revealed that two populations of plasma membrane vesicles were formed: large (176 +/- 22nm), and small (119 +/- 11nm). pu ...	1985	2993461
ileal loss of available carbohydrate in man: comparison of a breath hydrogen method with direct measurement using a human ileostomy model.	the breath hydrogen technique has suggested that a considerable amount of available plus unavailable carbohydrate enters the large intestine after the consumption of starchy foods (white bread 11%, wholemeal bread 8%, and red lentils 18%). direct measurement of the available carbohydrate in ileal effluent after the consumption of test meals by three individuals with ileostomies gave values similar to those determined by the breath hydrogen technique (white bread 10%, wholemeal bread 8%, and red ...	1986	3004195
molecular characterization of the egf receptor and involvement of glycosyl moieties in the binding of egf to its receptor on a clonal osteosarcoma cell line, umr 106-06.	the epidermal growth factor receptor in cells of the umr 106-06 clonal osteoblast line has been shown to be structurally similar to that previously characterized in other cell lines. a specific receptor component of approximately 165,000-185,000 mr has been identified by polyacrylamide gel electrophoresis using the chemical crosslinker disuccinimidyl suberate to crosslink 125i-egf to its receptor. tunicamycin treatment of cells resulted in a dose-dependent loss of binding suggesting involvement ...	1986	3006887
lectin histochemistry applied to human nerves.	histologic sections of normal and pathologically altered human peripheral nerves were stained with a battery of 20 fluorescein isothiocyanate-labeled lectins to determine whether these histochemical reagents could be used to identify peripheral nerve injury. eight plant lectins--from canavalia ensiformis, lens culinaris, triticum vulgare (wheat germ), types e4 and l4 from phaseolus vulgaris, types i and ii from ricinus communis, and wistaria floribunda--were found to bind to normal and pathologi ...	1986	3015069
affinity to lectin, biological and immunological characteristics of human chorionic gonadotropins from pregnant women and trophoblastic tumour patients.	to compare the affinity to lectin of human chorionic gonadotropins (hcg) from pregnant women and trophoblastic tumour patients, a small amount of urine was fractionated by a lentil lectin (lch) affinity chromatography. the lch-bound fractions were eluted with 2% mannoside solution, and each fraction was assayed for hcg activities by radioimmunoassay. in pregnant women, more than 90% of hcg immunoactivity in urine was bound to the lch column and eluted from it, whereas 8 to 26% and 37 to 51% of t ...	1986	3019056
inhibition of dna synthesis by a small-cell lung carcinoma-derived protein.	a tumor-derived factor that inhibits cellular dna synthesis was identified. the factor was extractable from a small-cell lung carcinoma cell line grown in either chemically defined medium or nu/nu mice and inhibited tritiated thymidine ([3h]dthd) incorporation by tumor cell lines of autologous, allogeneic, and xenogeneic origins. the viability of nonproliferating cells from normal tissue was not affected. tumor extract inhibitory activity was trypsin labile but was resistant to other proteases, ...	2010	3020301
effects of different proportions of carbohydrates, polysaccharides/monosaccharides, and different fibers on the metabolic control in diabetic rats.	recent findings have suggested that diets with a high level of carbohydrates may impair the metabolic control of diabetes mellitus in humans. moreover, other investigations have indicated that if the simple sugar content is increased in order to attain a proportion of polysaccharides/monosaccharides equal to 1, then neither the blood glucose nor the lipidic response show any change. we have studied the effect of increasing carbohydrates in the diet (59% v 82%), while maintaining cereal fiber lev ...	1986	3020344
interaction with lectin of kappa opioid binding sites solubilized from human placenta.	kappa opioid binding sites from human placenta, prelabeled with 3h-etorphine and solubilized, were retained on wheat germ agglutinin (wga) agarose and specifically eluted with n-acetylglucosamine. no significant retention was found with other immobilized lectins, including concanavalin a (con a), soybean seed lectin (sba), pisum sativum lectin (psa), lens culinaris medik. lectin (lca), and lathyrus tingitanus lectin(lta). about 23% of applied kappa sites were specifically eluted from wga agarose ...	1987	3027488
potentiating and suppressive ige-binding factors are expressed by a single cloned gene.	we have investigated expression of an ige-binding factor (ige-bf) cdna in both cos-7 monkey kidney cells and chinese hamster ovary cells. transient expression of the ige-bf clone in either cell type yielded ige-bf, which potentiated an in vitro ige response and had an affinity for lentil lectin. in contrast, when the transient expression experiments were carried out in the presence of tunicamycin, the factors no longer bound to lentil lectin. moreover, ige-bf expressed under these conditions sup ...	1987	3027707
the separation of immunocyte subpopulations by use of various lectins.	the use of lectins for the enrichment of various lymphocyte subpopulations was investigated. bauhinia purpurea lectin (bpa) was found to be effective for the enrichment of b cells, and the b cells thus obtained were further fractionated with lentil lectin into subsets showing high and low responses to dextran sulfate. the ability of various lectins to selectively induce suppressor t cell activity or helper t cell activity was also examined. the suppressor t cells thus induced were enriched peanu ...	1988	3051929
importance of glycosylation for hepatic clearance of renal renin.	three differently glycosylated forms of renin (renin a, b-1, and b-2) were highly purified from rat kidneys by pepstatin-aminohexyl-sepharose affinity chromatography and by serial lectin affinity chromatography on concanavalin a (con a) and lentil lectin-sepharose, and the role of glycosylation of renin was investigated. renin a and renin b-1 were loosely and tightly bound to con a, respectively, but did not bind to lentil lectin. renin b-2 bound to both con a and lentil lectin. these three form ...	1988	3056032
differently glycosylated multiple forms of renin in the blood circulation of rats.	serial lectin chromatography of rat plasma on concanavalin a and lentil lectin columns separated plasma renin into differently glycosylated multiple forms. to study the role of glycosylation in the clearance of circulating renin, three different forms of glycosylated renin (renin a, b-1 and b-2) were highly purified from rat kidneys. renin a and b-1 were loosely and tightly, respectively, bound to concanavalin a, but did not bind to lentil lectin. renin b-2 was bound to both concanavalin a and l ...	1988	3071581
administration of a 600 kd molecular fraction from pancreatic islets suppresses immune mediated diabetes in mice.	treatment of male c57bl6/j mice with low doses of streptozotocin causes the development of diabetes. infiltration of pancreatic islets by immune cells and the concomitant loss of beta islet cells can be prevented by immunosuppression. we now report that i.v. administration of islet homogenate 13 d prior to low dose streptozotocin treatment largely protects from development diabetes. homogenates from liver, kidney or exocrine pancreas were without effect. the suppressive activity was isolated par ...	1988	3075128
regulatory effects of human ige-binding factors on the ige response of rat lymphocytes.	normal human peripheral blood t cells were propagated in the presence of human interleukin 2, and activated cells were incubated with human ige-dimer to induce ige binding factor formation. the cells were then fused with a mutant of the human t cell line cem. five of the t cell hybridomas formed ige binding factors upon incubation with human ige-dimer. because ige binding factors formed by the human t cell hybridomas had affinity not only for human ige but also for rat ige, the biologic activiti ...	1986	3079805
monoclonal antibody affinity purification of a leishmania membrane glycoprotein and its inhibition of leishmania-macrophage binding.	specific monoclonal antibody coupled to affi-gel 10 was used to purify a major membrane glycoprotein of leishmania mexicana amazonensis, one of a group of parasitic protozoa that specifically infect mammalian macrophages. immobilized antigen was eluted at a 34% efficiency with buffers at either ph 2.5 or 11 or with mgcl2, but only the antigen eluted under basic conditions could be readsorbed to the immunobeads. sephacryl s-300 gel filtration of the purified antigen gave a single peak of protein ...	1986	3079902
inhibition of mitogenesis induced by phytohemagglutinin and lens culinaris lectin in adherent-cell supernatants treated with protein extract of mycobacterium tuberculosis.	specific stimulation of t cells by phytohemagglutinin and lens culinaris lectin was inhibited by a soluble factor(s) secreted by normal adherent cells stimulated with culture filtrate protein extract (cfpe) derived from bacterial cultures of mycobacterium tuberculosis h37ra (avirulent) and h37rv (virulent). the induction of the inhibitory factor was blocked by the presence of hyperimmune antisera to h37rv or h37ra cfpe. the inhibitory factor did not seem to be a cfpe reprocessed by the adherent ...	1986	3082760
chemical induction of sister-chromatid exchanges in human lymphocytes treated in g0 prior to stimulation by different mitogens and revealed 72 h later in second division cells.	frequencies of sister-chromatid exchanges (sce) were determined in second-division metaphases of human lymphocytes, exposed for 1 h during the g0 phase to mitomycin c (mmc) alone or to cyclophosphamide (cp) in the presence of s9 mix. the cells were then cultured for 72 h in the presence of phytohemagglutinin (pha), concanavalin a (con a), wistaria floribunda (wfa) or lens culinaris (lch-a) extracts. large differences in mitotic indices (mi) and cell-cycle kinetics were observed among cells subje ...	1986	3084967
interaction of trypanosoma cruzi with macrophages: effect of previous incubation of the parasites or the host cells with lectins.	the effect of incubation with lectins of the macrophages or two evolutive stages of trypanosoma cruzi (noninfective epimastigotes and infective trypomastigotes) on the ingestion of the parasites by mouse peritoneal macrophages was studied. lectins which bind to residues of mannose (lens culinaris, lca), n-acetyl-d-glucosamine or n-acetylneuraminic acid (triticum vulgaris, wga), beta-d-galactose (ricinus communis, rca), n-acetyl-d-galactosamine (phaseolus vulgaris, pha; dolichos biflorus, dba; an ...	1986	3085365
primary structure of a new tetraantennary glycan of the n-acetyllactosaminic type isolated from human factor viii/von willebrand factor.	n-glycosidically linked glycopeptides released by mild alkaline treatment of human factor viii/von willebrand factor (fviii/vwf) were fractionated by serial affinity chromatography on columns of sepharose linked to concanavalin a (cona) and lens culinaris agglutinin (lca). the fraction which is not retained on cona-sepharose, but eluted from lca-sepharose contains a pure minor glycopeptide which was structurally analysed. based on the results of methylation analysis and 500-mhz 1h-nmr spectrosco ...	1986	3089784
lectin-binding sites in normal, hyperplastic, adenomatous and carcinomatous human colorectal mucosa.	the carbohydrate structures of cellular glycoconjugates in normal, hyperplastic, adenomatous and carcinomatous human colorectal mucosa were analysed with six fluorescein isothiocyanate-conjugated lectins. in normal, hyperplastic and adenomatous colorectal mucosa showing mild or moderate dysplasia concanavalin a (con a), lens culinaris (lca), and wheat germ (wga) agglutinins stained goblet cell glycoconjugates (actual mucin goblet itself) while peanut (pna), vicia villosa (vva), and griffonia sim ...	1986	3092568
the human growth hormone receptor of cultured human lymphocytes. structural characteristics and glycosylation properties.	the structural characteristics and glycoprotein nature of the human growth hormone (hgh) receptor in cultured lymphocytes (im-9 cell line) were studied with the use of a bifunctional reagent (disuccinimidyl suberate) to couple 125i-hgh covalently to intact cells. after cross-linking, the hormone-receptor complexes were analysed by sodium dodecyl sulphate/polyacrylamide-gel electrophoresis. a single band of mr 140,000 was identified under reducing conditions. the labelling of this band was blocke ...	1986	3099769
glycoconjugates in retinoblastoma. a lectin histochemical study of ten formalin-fixed and paraffin-embedded tumours.	the binding of eleven biotin- or peroxidase-coupled lectins with different carbohydrate specificities to tumour tissue and remaining morphologically normal retina was studied in ten formalin-fixed and paraffin-embedded human eyes with retinoblastoma. in undetached retinas, outer and inner segments of photoreceptors bound concanavalin a (cona) as well as lens culinaris (lca), wheat germ (wga) ricinus communis (rcai) and peanut (pna) agglutinins. both nuclear and plexiform layers bound cona, lca a ...	1987	3105166
lectin binding patterns in amphibian epidermis.	a battery of 6 different horseradish peroxidase conjugated lectins has been employed for structural localization of glycoconjugates in amphibian epidermis. lens culinaris (lca) lectin stained the basal membrane and gave no significant reaction on the epidermal layers. canavlia ensiformis (con a) and griffonia simplicifolia ii (gs ii) lectins bound the keratinocyte cytoplasm and the basal membrane as well. ulex europaeus i (uea i) lectin had only reactivity with flask cells. griffonia simplifolia ...	1987	3105215
the generation of an organic free radical in substrate-reduced pig kidney diamine oxidase-cyanide.	when the cyanide complex of the copper protein, pig kidney diamine oxidase, is reduced anaerobically by cadaverine (1,5-diaminopentane), the broad, 480 nm, absorption band characteristic of the resting enzyme is bleached and a new absorption spectrum with features at 457, 429, 403 (shoulder), 360 (shoulder) and 332 nm appears. concomitantly, the epr spectrum of the enzyme cu(ii)-cn complex decreases in intensity and a new signal is observed that is attributable to an organic free radical. the g ...	1987	3106087
suppressive substance produced by t cells from mice chronically infected with trypanosoma cruzi. ii. partial biochemical characterization.	culture supernatants of splenic t cells from susceptible cba mice chronically infected with trypanosoma cruzi contain a suppressive substance which can inhibit the induction of delayed-type hypersensitivity (dth) to a wide range of antigens. the suppressive substance is distinct from t. cruzi antigen inasmuch as the supernatant depleted of any residual t. cruzi antigen by an affinity column still retains the suppressive activity, whereas addition of t. cruzi antigens to control supernatant did n ...	1988	3123553
[biosynthesis and main biological properties of human gamma interferon].	conditions for and regularities of gamma-interferon production were elucidated. high interferon inducing activity of the known inductors such as concanavalin a, phytohemagglutinin and lentil lectin was asserted. it was shown that aqueous and alcoholic extracts of legumes seeds were also able to induce interferon synthesis in cultures of human peripheral blood leukocytes. by physicochemical properties such as stability to acids and liability to heating and by antigenic properties, interferon prod ...	1988	3132115
localization of cortical granule constituents before and after exocytosis in the hamster egg.	electrical activation of the hamster egg was used to study cortical granule constituents before and after exocytosis. the activated hamster eggs underwent cortical granule decondensation just prior to and at the time of exocytosis. some of the cortical granules of aged, unactivated eggs underwent similar changes. fitc- and gold-conjugated lens culinaris agglutinin (lca) bound intensely to the surfaces of activated but not unactivated eggs. this labelling was associated with the microvilli. perme ...	1988	3133448
a burst-promoting activity derived from the human bone marrow stromal cell line km-102 is identical to the granulocyte-macrophage colony-stimulating factor.	recently, several human bone marrow stromal cell lines have established and produced hematopoietic growth factors. one of these factors, a burst-promoting activity (bpa), was purified from 6 liters of serum-free conditioned medium cultured from stromal cell line km-102, which was stimulated by phorbol myristate acetate (pma) and calcium ionophore a23187. this stimulation induced 60 times more production of bpa than the unstimulated control culture. bpa was purified 4000-fold by sequential fracti ...	1988	3134250
quantitative studies of changes in cortical granule number and distribution in the mouse oocyte during meiotic maturation.	cortical granules (cgs) undergo a substantial change in distribution in the mouse oocyte cortex during meiotic maturation. in order to determine the mechanism of their change in distribution near the time of ovulation, cg density, total number per oocyte, and domain areas were quantitated. cgs were visualized microscopically by lens culinaris agglutinin-biotin and texas red-strepavidin fluorescence as well as by electron microscopy. immature germinal vesicle stage (gv) oocytes from adult mice ha ...	1988	3141231
purification of a murine leukemia inhibitory factor from krebs ascites cells.	a factor capable of inducing terminal differentiation in the murine myeloid leukemia cell line m1 has been purified to apparent homogeneity from the medium conditioned by krebs ii ascites tumor cells. the factor, termed leukemia inhibitory factor (lif) is a single chain glycoprotein of apparent mr 58,000 which induces differentiation and inhibits proliferation of the m1 cell line but not the wehi-3b d+ murine myeloid leukemic cell line and has no detectable proliferative activity on normal myelo ...	1988	3142300
surface carbohydrate changes on onchocerca lienalis larvae as they develop from microfilariae to the infective third-stage in simulium ornatum.	use was made of seven fitc labelled lectins as tools to investigate the surface of onchocerca lienalis larvae as they develop through to the infective third-stage in a natural vector, simulium ornatum. the lectins were derived from canavalia ensiformis (con a), lens culinaris (lentil), triticum vulgaris (wheat germ), arachis hypogaea (peanut), helix pomatia, phaseolus vulgaris (kidney bean) and tetragonolobus purpureus (asparagus pea). between 70 and 100 living parasites were examined for each d ...	1988	3142948
combined use of lectin affinity chromatography and endo-beta-galactosidase to study polylactosamine sequences isolated from haemopoietic cell surfaces.	the trypsin-sensitive glycopeptides from cell surfaces of a multipotential murine haemopoietic cell line (de) have been studied using serial lectin affinity chromatography on columns of immobilized lentil lectin (lca), concanavalin a (con a), and wheat-germ agglutinin (wga). wga-binding material consisted of glycopeptides that failed to bind to lca and con a. step elution from the wga-column with 0.01-, 0.1-, 0.5- and 1.0 m n-acetyl-d-glucosamine yielded four affinity classes of glycopeptide (wg ...	2011	3147728
glycoconjugates of the human sperm surface: distribution and alterations that accompany capacitation in vitro.	we have studied changes in the binding of fluoresceinated lectins to human sperm during in vitro capacitation. we first determined the surface labeling pattern of viable sperm obtained by the swim-up procedure. sperm were labeled with 100 micrograms/ml fitc-conjugated lectin at 4 degrees c for 30 min. we simultaneously used hoechst stain 33258 as a supravital stain to help differentiate surface from intracellular lectin labeling. of 14 lectins studied, six (phytohemagglutinin-e, concanavalin a, ...	1987	3147937
characterization of a soluble protein of coccidiodes immitis with activity as an immunodiffusion-complement fixation antigen.	a 48-kilodalton (kda) electrophoretically distinct antigen from coccidioides immitis mycelial- and spherule-endospore-phase filtrates was previously associated by immunoblotting with the immunodiffusion band that corresponds to complement-fixing activity (id-cf). to characterize this antigen and its precursor, both mycelial- and spherule-endospore-phase filtrates were fractionated by size exclusion chromatography, lectin affinity chromatography, and nondenaturing electrophoresis. by size exclusi ...	1988	3148631
isolation of a subpopulation of glycoprotein iib-iii from platelet membranes that is bound to membrane actin.	triton x-100-insoluble residues, or skeletons, of plasma membrane-rich vesicles obtained from unstimulated human platelets were isolated by high speed centrifugation. about 10-15% of the total surface iodinatable glycoproteins iib and iii (gpiib and gpiii, respectively) co-isolated with the insoluble fraction. after sonication and centrifugation the solubilized material was further purified by affinity chromatography on lens culinaris lectin-sepharose. sds page analysis of this material revealed ...	1985	3155751