Publications

rapid screening method for deoxynivalenol in agricultural commodities by fluorescent minicolumn.	a rapid screening procedure based on the selective adsorption of deoxynivalenol (don) from extracts of wheat and corn has been developed. don is extracted from the sample with acetonitrile-water (85 + 15) and partially purified on a preparative minicolumn. solvent is evaporated and the residue is dissolved in toluene-acetone (95 + 5) and chromatographed on a novel detector minicolumn which selectively adsorbs don. a blue fluorescence is produced when the column is heated 5 min at 100 degrees c. ...	2015	2324037
identification and partial characterization of the soybean-dust allergens involved in the barcelona asthma epidemic.	asthma epidemics in barcelona, spain, have been attributed to dust generated by the unloading of soybeans in the harbor. sera of four different groups of 10 subjects in each group were studied: (1) patients attending an emergency room in barcelona for an asthma attack on epidemic days, group a, (2) patients attending an emergency room for an attack on nonepidemic days, group b, (3) patients with asthma from other cities, group c, and (4) patients without asthma from barcelona matched by age and ...	1990	2324415
characterization of expressed meiotic prophase repeat transcript clones of lilium: meiosis-specific expression, relatedness, and affinities to small heat shock protein genes.	the inserts of plasmid cdna clones for transcripts showing meiotic prophase specific expression show cross reassociation to varying degrees of intensity with one another. these clones were recovered from a cdna library made from lilium microsporocyte poly(a)+ rna. rna-dot and northern-blot analyses indicate that these clones represent transcripts specific to the meiotic prophase interval in microsporocytes. the transcripts appear to constitute the most abundant class of meiosis-specific poly(a)+ ...	1990	2332163
purification and characterization of a novel calcium-dependent protein kinase from soybean.	a novel calcium-dependent protein kinase (cdpk) previously reported to be activated by the direct binding of ca2+, and requiring neither calmodulin nor phospholipids for activity [harmon, a.c., putnam-evans, c.l., & cormier, m.j. (1987) plant physiol. 83, 830-837], was purified to greater than 95% homogeneity from suspension-cultured soybean cells (glycine max, l. wayne). purification was achieved by chromatography on deae-cellulose, phenyl-sepharose, sephadex g-100, and blue sepharose. the puri ...	1990	2334677
[the evaluation of the genotoxic effects of samples of industrial and domestic sewage. ii. the mutagenic and recombinant effects of sewage in the soybean (glycine max. (l.)) test system].	the genotoxicity of the industrial and domestic sewage has been studied on the soybean test-system glycine max (l.). it makes it possible to take into account different types of genetic disturbances according to appearance of various spot types on sprout leaves (somatic mosaicism).	1990	2346766
nodule formation in soybeans by exopolysaccharide mutants of rhizobium fredii usda 191.	production of exopolysaccharides by rhizobium has been linked with efficient invasion and nodulation of leguminous plant roots by the bacteria. exopolysaccharide-deficient (exo) mutants of rhizobium fredii usda 191 were isolated following tn5-insertion mutagenesis. five phenotypically unique exo mutants were investigated for exopolysaccharide synthesis and their ability to nodulate soybeans. the exopolysaccharides produced by these mutants were analysed for polysaccharide composition by column c ...	1990	2351951
subunit and amino acid composition of diacylglycerol acyltransferase from germinating soybean cotyledons.	the subunit and amino acid composition of the enzyme that catalyses triacylglycerol synthesis was determined for the first time from plant tissues. diacylglycerol acyltransferase (acyl-coa:1,2-diacylglycerol o-acyltransferase, ec 2.3.1.20) purified from germinating soybean (glycine max l. merr. cv. dare) cotyledons, dissociated into three nonidentical subunits having apparent molecular masses of 40.8, 28.7, and 24.5 kda. the respective subunits occurred in a 1:2:2 molar ratio in the native enzym ...	1990	2354202
[interpretation of thermograms of protein denaturation in non-equilibrium conditions].	data are presented concerning the effect of heating rate on the denaturation parameters of small and oligomeric globular proteins: kunitz trypsin inhibitor from soybeans and 1,5-ribulose bisphosphate carboxylase from tobacco leaves. substantional dependence of denaturation temperature on the heating rate reflects non-equilibrium pattern of denaturation of these proteins under experimental conditions. to interpret these data a kinetic approach is proposed, which permits determination of equilibri ...	1990	2369595
protease inhibitor suppression of colon and anal gland carcinogenesis induced by dimethylhydrazine.	in the present study, we examined the ability of chymostatin, a highly specific inhibitor of chymotrypsin, to suppress dimethylhydrazine-induced colon carcinogenesis, and the dose-response relationship for an extract of soybeans containing the bowman-birk inhibitor (bbi) to suppress dimethylhydrazine-induced colon carcinogenesis, when added to the diet of mice. our results showed that: (i) diets containing 0.1% bbi reduced the incidence of adenocarcinomas of the colon approximately 50%, but had ...	1990	2372868
the source and content of vitamin b12 in the tempehs.	vitamin b12 contents were determined on 10 commercial tempeh samples purchased from various markets in jakarta, indonesia. a relatively high vitamin b12 content was found, i.e., 19 ng/g (ranges from 1.8 to 41.4 ng/g). as soybeans contain no vitamin b12, the amount of vitamin in the tempeh must therefore be derived from the other sources during the fermentation process. the tempeh prepared in the laboratory by inoculation of the commercial starter into the sterile soybean contained a much higher ...	1990	2380647
comparison of the carbohydrate-binding specificities of seven n-acetyl-d-galactosamine-recognizing lectins.	seven plant lectins, dolichos biflorus agglutinin (dba), griffonia simplicifolia agglutinin (gsa, isolectin a4), helix pomatia agglutinin (hpa), soybean (glycine max) agglutinin (sba), salvia sclarea agglutinin (ssa), vicia villosa agglutinin (vva, isolectin b4) and wistaria floribunda agglutinin (wfa), known to be specific for n-acetyl-d-galactosamine-(galnac) bearing glycoconjugates, have been compared by the binding of their radiolabelled derivatives, to eight well-characterized synthetic oli ...	2008	2384093
differences in expression of oligosaccharide determinants by phenotypically distinct sublines of the dunning 3327 rat prostate cancer.	oligosaccharides expressed by the 3327-h and 3327-mat lylu sublines of the dunning rat prostate cancer model have been compared in formalin-fixed and routinely paraffin-embedded tumour tissues. binding by lectins of defined specificity has been employed to identify expression of seven oligosaccharide structures by primary and metastatic prostatic carcinoma cells. neuraminidase digestion was employed to reveal determinants masked by sialic acid. the presence of core man alpha 1----3(man alpha 1-- ...	1990	2388349
production of enterotoxin a and thermonuclease by staphylococcus aureus in legumes.	growth, enterotoxin a (sea) and thermonuclease (tnase) production of s. aureus (strains cp 7 and fri 722) was determined in media produced from the following heat or irradiation sterilized legumes: peas, black beans, mung beans, adzuki beans and soybeans. media containing the five legumes alone or in combination with brain heart infusion broth (bhi) were tested. with the exception of heat-sterilized black beans and adzuki beans, s. aureus growth was excellent in all media with cell counts after ...	1990	2397154
isolation of a cdna clone for pea (pisum sativum) seed lipoxygenase.	a lipoxygenase cdna clone, pcd45, was identified in a pisum sativum l. (pea) seed mrna cdna library by hybrid-release/translation followed by immunoprecipitation with antiserum raised against lipoxygenase from glycine max l. (soya bean). pcd45 hybrid-selected an mrna encoding the larger of the two polypeptides of mr approximately 95 000 that were immunoprecipitated from cell-free translation products of pea seed poly(a)-containing rna by the g. max anti-lipoxygenase. 'northern'-blot analysis sho ...	1983	2417590
improved procedure for the detection of activity of lipoxygenases on electropherograms.	several procedures for activity staining of lipoxygenases were compared. the best results were obtained with the o-dianisidine method of de lumen and kazeniac which had to be modified, however, with respect to the conditions of electrophoretic separation, treatment of the gel after separation, incubation conditions and staining solution, to achieve satisfactory detection of the lipoxygenases from wheat grains, pea seeds, soybeans and rabbit reticulocytes.	1985	2417596
new approaches to the study of cellulose biosynthesis.	examples of a variety of approaches for studying the mechanism and regulation of cellulose biosynthesis are presented. attempts to demonstrate conclusively a cellulose synthase activity using membrane preparations derived from higher plants have not been successful; the predominant udp-glucose: beta-glucan-beta-glucosyltransferase detected in these preparations is a beta-(1----3)-glucan synthase that is dependent upon ca2+ and a beta-glucobiose, such as laminaribiose or cellobiose, for activity. ...	1985	2419349
monoclonal antibodies directed against protoplasts of soybean cells: analysis of the lateral mobility of plasma membrane-bound antibody mvs-1.	a monoclonal antibody (mvs-1) was used to monitor the lateral mobility of a defined component (mr approximately 400,000) of the plasma membrane of soybean protoplasts prepared from suspension cultures of glycine max (sb-1 cell line). the diffusion coefficient (d) of antibody mvs-1 bound to its target was determined (d = 3.2 x 10(-10) cm2/s) by fluorescence redistribution after photobleaching. pretreatment of the protoplasts with soybean agglutinin (sba) resulted in a 10-fold reduction of the lat ...	1986	2420806
evidence for glycoconjugate in nociceptive primary sensory neurons and its origin from the golgi complex.	glycoconjugates with terminal galactose residues were localized in rat spinal cord and spinal ganglia using lectin-hrp conjugates of griffonia simplicifolia and glycine max agglutinins. alternate staining of serial sections with hrp-labelled lectins and an antibody for substance p (sp) showed staining in identical primary sensory neurons with both methods. similarly, lectin-reactive as well as sp-positive fibers were found in rexed laminae i and ii, lissauer's tract, the spinal nucleus and tract ...	1986	2425897
functional analysis of regulatory elements in a plant embryo-specific gene.	previously we demonstrated the expression of a plant embryo-specific gene encoding the alpha' subunit of beta-conglycinin, a seed storage protein of soybean (glycine max), in transgenic petunia plants. to examine the regulatory elements that control the expression of this embryo-specific gene (gmg17.1), a series of deletion mutants was made that contain the alpha'-subunit gene flanked in the 5' direction from +14 nucleotides to -8.5 kilobases (kb) relative to the site of transcription initiation ...	1986	2430294
effect of a trypsin inhibitor from soybeans (bowman-birk) on the secretory activity of the human pancreas.	this study was undertaken to clarify the issue of whether feedback regulation of pancreatic enzyme secretion occurs in humans. a naturally occurring inhibitor of trypsin and chymotrypsin, the bowman-birk inhibitor of soybeans, was used to reduce the activities of these enzymes normally secreted by the pancreas into the duodenum. pure pancreatic juice was collected by endoscopic retrograde cannulation of the pancreatic duct in a protocol consisting of three periods: period 1 (15 min), collections ...	1988	2446949
two host-inducible genes of rhizobium fredii and characterization of the inducing compound.	random transcription fusions with mu d1(kan lac) generated three mutants in rhizobium fredii (strain usda 201) which showed induction of beta-galactosidase when grown in root exudate of the host plants glycine max, phaseolus vulgaris, and vigna ungliculata. two genes were isolated from a library of total plasmid dna of one of the mutants, 3f1. these genes, present in tandem on a 4.2-kilobase hindiii fragment, appear in one copy each on the symbiotic plasmid and do not hybridize to the rhizobium ...	1988	2447061
light microscopic characterization of glycoconjugates in secretory cells of the carp (cyprinus carpio) gill epithelium.	secretory products of granular and mucous cells in the gill epithelium of the carp, cyprinus carpio, were distinguished by their cytochemical reactions with peroxidase-labelled lectins and with the galactose oxidase (go)-schiff reagents. secretory products of granular cells reacted with lectins from triticum vulgaris (wga), arachis hypogaea (pna), dolichos biflorus (dba), glycine max (sab), and lotus tetragonolobus (lta). they also reacted with go-schiff reagents. after sialic acid cleavage with ...	1987	2449406
high and low inhibitor soybean meals affect human duodenal proteinase activity differently: in vivo comparison with bovine serum albumin.	the objective of this study was to investigate the effects of high and low inhibitor soybean meals on the duodenal enzyme activities and on the possible regulatory role of gastrointestinal hormones in the pancreatic response. after an overnight fast, 11 healthy volunteers received an intraduodenal infusion of saline for 60 min. this was followed by infusion of either of three test meals: extract of raw soybeans (rs), a low inhibitor soy protein isolate (spi) or bovine serum albumin (bsa), 10 g/h ...	1988	2451718
glycoconjugates in normal human kidney. a histochemical study using 13 biotinylated lectins.	the avidin-biotin-peroxidase complex technique was used with 13 lectins to study the glycoconjugates of normal human renal tissue. the evaluated lectins included triticum vulgaris (wga), concanavalin ensiformis (cona), phaseolus vulgaris leukoagglutinin and erythroagglutinin (pha-l and pha-e), lens culinaris (lca), pisum sativum (psa), dolichos biflorus (dba), glycine max (sba), arachis hypogaea (pna), sophora japonica (sja), bandeiraea simplicifolia i (bsl-i), ulex europaeus i (uea-i) and ricin ...	1988	2466021
o-antigen from bradyrhizobium japonicum lipopolysaccharide inhibits intercellular (symplast) communication between soybean (glycine max) cells.	the technique of fluorescence redistribution after photobleaching was utilized to measure intercellular movement of low molecular weight fluorescent hydrophilic substances across the cell wall/membrane interface between contiguous soybean (glycine max (l.) merr. cv. mandarin) root cells (sb-1 cell line) in tissue culture. lipopolysaccharide (lps) purified from bradyrhizobium japonicum r110d, a gram-negative bacterium that normally infects and induces nodulation in soybean roots in vivo, inhibits ...	1989	2473070
differential regulation of soybean chalcone synthase genes in plant defence, symbiosis and upon environmental stimuli.	four independent recombinant lambda clones hybridizing to parsley chalcone synthase (chs) cdna were isolated from a soybean (glycine max) genomic library. restriction fragment length polymorphism (rflp) analysis indicated that the chs gene family comprises six members. the chs genes were found to be clustered with three genes on a 10 kb segment and pairs on others. dna sequences of the 5'-, the coding-, and the 3' untranslated regions were determined for three different genes. a consensus alignm ...	1989	2476656
characterization of the glycinin gene family in soybean.	we characterized the structure, organization, and expression of genes that encode the soybean glycinins, a family of storage proteins synthesized exclusively in seeds during embryogenesis. five genes encode the predominant glycinin subunits found in soybeans, and they have each been cloned, sequenced, and compared. the five genes have diverged into two subfamilies that are designated as group-i and group-ii glycinin genes. each glycinin gene contains four exons and three introns like genes that ...	1989	2485233
isolation and partial characterization of the cell membrane of walker-256 carcinosarcoma ascites cells.	cell membranes of carcinosarcoma-256 ascites cells were isolated by the zn++ method. neutral sugars, sialic acid, fucose and n-acetylhexosamines were determined in the cell membrane fraction. cells were agglutinated by wheat germ agglutinin, concanavalin a, and the lectins from phaseolus vulgaris and glycine max. no agglutination was observed with peanut lectin (arachis hypogaea). gel electrophoresis under dissociating conditions revealed more than 40 bands in the membrane fraction after silver ...	1989	2486452
phytoalexin synthesis in soybean cells: elicitor induction of reductase involved in biosynthesis of 6'-deoxychalcone.	chromatofocusing on mono p proved to be an efficient purification procedure for the nadph-dependent reductase from soybean (glycine max l.) cell cultures which acts together with chalcone synthase in the biosynthesis of 2',4',4-trihydroxychalcone (6'-deoxychalcone). by isoelectric focusing the pi of reductase was determined to be 6.3. addition of pure soybean reductase to cell-free extracts from stimulated cell cultures of parsley and bean (phaseolus vulgaris) and from young flowers of dahlia va ...	1989	2500065
detection of desmethyldiazepam and diazepam in brain of different species and plants.	recent data suggest that desmethyldiazepam (dd), a major metabolite of several benzodiazepines (bzd), might be of natural origin. therefore we tried to quantify dd and diazepam (d) in animals during maturation (e.g. hen, chicken, eggs), in brain of species at different evolutionary stages e.g. salmon, frog, monitor/reptile, rat, cat, dog, deer, bovine) including newborn and adult humans. since low concentrations of dd (range 0.01-0.04 ng/g wet wt) and d (range 0.005-0.02 ng/g) could be measured ...	1989	2502983
influence of maternal diet during lactation and use of formula feeds on development of atopic eczema in high risk infants.	to examine the effects of maternal diet during lactation and the use of formula feeds on the development of atopic eczema in infants at risk.	1989	2504375
regional distribution of glycoconjugates in normal, transitional and neoplastic human colonic mucosa. a histochemical study using lectins.	regional distribution of glycoconjugates in normal and neoplastic colonic mucosa was studied by means of eight lectins: dolichos biflorus (dba), glycine max (sba), triticum vulgare (wga), arachis hypogaea (pna), griffonia simplicifolia-i (gs-i), canavalia ensiformis (con a), limax flavus (lfa), and ulex europaeus-i (uea-i). the lectin binding patterns were examined in 40 normal colonic mucosa (nm) (12 proximal (p) and 28 distal (d], 38 carcinomas (15 p and 23 d), and 31 transitional mucosa (tm) ...	1989	2505446
phytoalexin synthesis in soybean: purification and reconstitution of cytochrome p450 3,9-dihydroxypterocarpan 6a-hydroxylase and separation from cytochrome p450 cinnamate 4-hydroxylase.	elicitor-challenged soybean (glycine max) cell cultures were used for detergent solubilization and purification of cytochrome p450 3,9-dihydroxypterocarpan 6a-hydroxylase (d6ah). d6ah was purified to electrophoretic homogeneity from such cells by a five-step procedure. it could be separated from cytochrome p450 cinnamate 4-hydroxylase on hydroxyapatite. this is the first report on separation of two cytochrome p450 enzymes from a higher plant. on sodium dodecyl sulfate polyacrylamide gels d6ah mi ...	1989	2505672
phylogenetic relationships between the chlorophyll a/b binding protein (cab) multigene family: an intra- and interspecies study.	the genome of glycine max (l.) merr. cv. "dare" contains a chlorophyll a/b binding (cab) protein gene family consisting of 10 genes. the primary structures of two linked cab genes (cab 4 and cab 5) were determined. a comparison of the nucleic acid and predicted amino acid sequences of cab 4 and cab 5 revealed a high degree of similarity (96% and 98%, respectively). phylogenetic inferences drawn from sequence comparisons between previously characterized soybean cab 1, 2, and 3 and cab 4 and 5 sug ...	1989	2506360
the cdna cloning of a pea (pisum sativum) seed lipoxygenase. sequence comparisons of the two major pea seed lipoxygenase isoforms.	cloning and sequencing of two cdnas from mrna of maturing pea (pisum sativum) seeds allowed the deduction of the complete amino acid sequence of a lipoxygenase polypeptide which is most similar to that of soya-bean lipoxygenase 2. the predicted mr of this polypeptide is 97134, and its sequence permits comparisons between the lox2-type and the lox3-type lipoxygenase isoforms from pea and soya bean (glycine max).	1989	2515855
the microbial ecology of soybean soaking for tempe production.	soybeans soaked in tap water for 24 to 36 h at 20, 30 or 37 degrees c underwent a natural fermentation that was characterized by the growth of microorganisms to 10(8)-10(10) cfu/ml (depending on temperature) and a reduction of ph from 6.5 to 4.5. lactobacillus casei, streptococcus faecium, staphylococcus epidermidis and streptococcus dysgalactiae dominated the fermentation but, significant contributions were also made by klebsiella pneumoniae, klebsiella ozaenae, enterobacter cloacae, enterobact ...	1989	2518226
nodule-specific kinases phosphorylating nuclear factors in isolated nuclei.	in vitro phosphorylation of total nuclear proteins from soybean (glycine max l) nodules formed by bradyrhizobium japonicum 61a76 showed several differences in comparison with those from uninfected roots or embryonic-axes nuclei. three types of protein phosphorylations were observed in nodule nuclei: ca(2+)- and calmodulin-independent, ca(2+)- and calmodulin-dependent, and ca(2+)-dependent but calmodulin-independent. in addition, ca(2+)-dependent dephosphorylation of some nuclear proteins was obs ...	1989	2535508
changes in differently processed soya bean (glycine max.) and lima bean (phaseolus lunatus) with particular reference to their chemical composition and their mineral and some inherent anti-nutritional constituents.	the effects of 3 processing techniques: cooking, roasting and autoclaving on the proximate chemical composition, the mineral content and some inherent toxic factors of soya and lima beans were investigated. the processing techniques generally reduced the crude fibre levels and enhanced the extractable fat in the soya and lima beans. the coefficients of variability for crude fibre and ether extract due to the processing techniques of soya and lima beans were 20.9%, 16.0% and 22.3%, 38.1%, respect ...	1989	2561304
selective staining of a population of parvalbumin-containing gabaergic neurons in the rat cerebral cortex by lectins with specific affinity for terminal n-acetylgalactosamine.	lectins with specific affinity for terminal n-acetylgalactosamine, vicia villosa agglutinin (vva) and glycine max agglutinin (soybean agglutinin; sba), are shown to stain selectively a subpopulation of gabaergic neurons in the rat cerebral cortex. about 90% of vaa- and/or sba-stained cells are also parvalbumin-immunoreactive, but no vva-stained cells showed somatostatin-28-like or cholecystokinin-8-like immunoreactivities.	1989	2565147
genetic variation in an inbred plant: variation in tissue cultures of soybean [glycine max (l.) merrill].	although soybean [glycine max (l.) merrill] grows as an inbreeding, generally homozygous, plant, the germplasm of the species contains large amounts of genetic variation. analysis of soybean dna has indicated that variation of rflp (restriction fragment length polymorphism) markers within the species usually entails only two alleles at any one locus and that mixtures of such dimorphic loci account for virtually all of the restriction fragment variation seen in soybean (g. max), and in its ancest ...	1989	2567263
resolution of the isoenzymes of soybean lipoxygenase using isoelectric focusing and chromatofocusing.	isoelectric focusing in thin-layer polyacrylamide gels has been applied to the analysis of the enzymes involved in the formation and destruction of peroxides in soybeans [glycine max (l.)], lipoxygenases and peroxidases, respectively. as a result of differences in ph optima for catalytic activity, lipoxygenases were selectively detected by adjusting the ph employed for activity-specific staining. type-1 lipoxygenase was revealed not only by staining based on the conversion of linoleic acid to hy ...	1985	2581473
dynamic changes of cell-surface glycoconjugates in human palmar epidermis following friction-blisters.	damage and repair of cell-surface glycoconjugates were examined in human palmar skin following friction-blister injury, using biotinylated lectins and the avidin-biotin complex method. in normal skin, concanavalin a, ricinus communis, and triticum vulgaris bound to the surface of cells from the basal layer to the granular layer. after injury, binding of concanavalin a was absent in the plasma membrane, but appeared in the cytoplasm at perinuclear sites. the surface reaction was recovered in basa ...	1989	2582482
crystal structure of recombinant human interleukin-1 beta at 2.0 a resolution.	the crystal structure of recombinant human interleukin-1 beta (il-1 beta) has been determined at 2.0 a resolution and refined to a crystallographic r-factor of 0.19. three heavy-atom derivatives were identified and used for multiple isomorphous replacement phasing. interpretation of the resulting electron density map revealed a structure in which there are 12 antiparallel beta-strands and no alpha-helix. the single 153-residue polypeptide chain is folded into a six-stranded beta-barrel similar i ...	1989	2585509
[the investigation of asthmatic children by multiple factor analysis. i. the relation between severity of asthma and allergic factors].	in a previous study, we analysed the severity of bronchial asthma in childhood by the class ii multiple factor analysis method. at that time, the titers of ige and igg4 antibodies to the three major food (cow's milk, egg white, and soybeans) and inhalation antigens such as house dust and mites were, however, left unevaluated because of a lack of independence. the quantification of their relative ratios was introduced into the present study, which made it possible to compare their individual pote ...	1989	2589988
the sequence of the chloroplast 5s ribosomal rna gene of soybean (glycine max merr.).		1989	2602127
characterization of a neuraminidase from corynebacterium aquaticum responsible for th polyagglutination.	th polyagglutinability is characterized by the agglutination of the red blood cells (rbc) by arachis hypogaea, medicago disciformis, vicia cretica but, in contrast to the t phenomenon, not by glycine max (glycine soja). because th transformation of rbc has been obtained in vitro, the mechanism of th polyagglutinability expression has been studied and reproduced experimentally. an enzyme with neuraminidase specificity has been isolated from the culture supernatant of corynebacterium aquaticum, an ...	1989	2617954
lectin binding to small intestinal goblet cells of newborn, suckling, and weaned pigs.	lectin binding of small intestinal goblet cells was examined in newborn, suckling, and weaned pigs. sections of duodenum, proximal portion of the jejunum, distal portion of the jejunum, and ileum were embedded in a hydrophilic acrylic resin and treated with each of the following lectins: canavalia ensiformis, ricinus communis i, glycine max, ulex europaeus i, and triticum vulgaris. percentages of goblet cells binding each lectin were calculated within intestinal regions. differences in lectin-bi ...	1989	2619128
[nucleotide sequences encoding glycinin b4 polypeptide of the cultivated soybean glycine max and its presumed wild-type ancestor glycine soja in connection with the origin of cultivated soybean].	nucleotide sequences of cdnas encoding soybean glycinin b4 polypeptide were compared in three soybean cultivars and two plant introductions of wild soybean glycine soja. only two nucleotide substitutions were found in three cultivars g. max, as compared with g. max and g. soja having nucleotide sequences which contain four nucleotide substitutions. these data serve as additional evidence, at molecular level, indicating the origin of g. max from g. soja. on the other hand, the time period require ...	1989	2625198
[a study on the effect of fermented soybean in preventing iron deficiency anemia in children].	using the fermenting technique that hydrolysis the phytate in soybeans. the absorption rate of iron was improved and a series of fermented soya foods were produced. feeding the fermented food to 437 children for 6 months, the incidence of ida children significantly decreased from 21.7% to 1.25%. the iron absorption rate (21.8%) in the children given the diet containing fermented soy was significantly higher than that of (14.2%) in the children given the bland diet (control group) and about the s ...	1989	2627849
purification and characterization of trypsins from the digestive tract of locusta migratoria.	two trypsin-like enzymes were isolated from the digestive tract of the african migratory locust locusta migratoria migratorioides. primary purification was carried out on a deae-cellulose column, from which the two trypsins emerged in the anionic fraction. further purification was achieved by affinity chromatography on a p-aminobenzamidine (paba)-sepharose column, which also separated the two trypsins (tleaff.1. and tleaff.2.), or by hplc on an anion exchange column. the purity and homogeneity o ...	1989	2635697
fusarium moniliforme sheld. a fungus producing a broad spectrum of bioactive metabolites.	fusarium moniliforme sheldon (perfect stage gibberella fujikuroi (saw. wollenweber), an ubiquitous fungus belonging to the section liseola (booth), is one of the widespread, phytopathogenic fungi. this field fungus common on many crops, including rice, oats, wheat, maize, barley and soybeans was well known because of the ability to produce gibberellins in high quantities. today beside of the very important gibberellic acid (ga3) other gibberellins such as ga4 and ga7 are of significance for agri ...	1989	2652945
estimation of nitrogenase activity in the presence of ethylene biosynthesis by use of deuterated acetylene as a substrate.	nitrogenase reduces deuterated acetylene primarily to cis dideuterated ethylene. this can be distinguished from undeuterated ethylene by the use of fourier transform infrared spectroscopy. characteristic bands in the region from 800 to 3,500 cm-1 can be used to identify and quantitate levels of these products. this technique is applicable to field studies of nitrogen fixation where ethylene biosynthesis by plants or bacteria is occurring. we have verified the reaction stoichiometry by using kleb ...	1989	2655535
[evaluation of allergen-specific ige antibodies by a newly developed mast allergy system].	mast, which stand for multiple antigen simultaneous test, uses enzyme-linked anti-human ige and chemiluminogenic substate to determine ige. this system is characterized by simultaneous analysis of multiple allergen items, up to 35, together with total ige determination. we evaluated usefulness of this mast system using 191 serum samples obtained from patients with bronchial asthma, allergic rhinitis and/or atopic dermatitis. it was found that there were statistically significant correlations bet ...	1989	2686587
lectin-binding sites in neoplastic and non-neoplastic colonic mucosa of 1,2-dimethylhydrazine-treated rats.	qualitative changes of glycoconjugates in luminal surface and goblet cell mucin from colon mucosa of 1,2-dimethylhydrazine (dmh)-treated rats were studied. eight fluoresceinated lectins were used: dolichos biflorus (dba), glycine max (sba), triticum vulgare (wga), limax flavus (lfa), arachis hypogaea (pna), griffonia simplicifolia-i (gs-i), ulex europaeus-i (uea-i) and canavalia ensiformis (con a). the lectin-binding patterns were studied in tumors arising in proximal and distal portions of the ...	1989	2689774
characterization of phytochrome-regulated gene expression in a photoautotrophic cell suspension: possible role for calmodulin.	a photoautotrophic suspension culture of soybeans was found to exhibit light-dependent expression of the genes encoding the major chlorophyll a- and b-binding protein (cab). the expression was mediated by phytochrome, since it was induced by red light and reversed by far-red light. the maximal level as well as the kinetics of the induction were comparable between the suspension culture and soybean seedlings. using this cell culture, we addressed the question of whether a calcium- and/or calmodul ...	1989	2689868
apparent digestibility of nitrogen and amino acids in protein feedstuffs by growing pigs.	eight digestion trials were conducted with pigs fitted with ileal t-cannulas to determine the apparent digestibilities of n and amino acids in common protein feedstuffs. trial-to-trial variation was minimal as determined by the variation in digestibilities for soybean meal, which was evaluated in each trial. apparent digestibilities varied widely; ileal n digestibility ranged from 88 to 63%, and ileal lysine digestibility ranged from 93 to 40%. in general, ring-dried blood meal, corn gluten meal ...	1989	2703444
determination of fluazifop-butyl and fluazifop acid in soybeans and soybean oil using liquid chromatography with oxidative amperometric detection.	a new method is described for the determination of the herbicide fluazifop-butyl, and its metabolite fluazifop acid, in soybeans and soybean oil as fluazifop acid. liquid chromatography with amperometric detection (lc/ad) is used to determine fluazifop acid produced from the metabolism or base hydrolysis of fluazifop-butyl in soybeans and soybean oil. these foods were spiked with fluazifopbutyl at 0.05, 0.10, and 0.50 ppm and hydrolyzed with 0.2n naoh in methanol. the hydrolysate (adjusted to ph ...	2016	2708288
enzyme-linked immunosorbent assay and immunoblotting determination of antibody response to major component proteins of soybeans in patients with soy protein intolerance.	to evaluate the role of food-specific antibody response to soy protein and its fractions in patients with soy protein intolerance, allergen-specific igg and ige to these proteins were measured by enzyme-linked immunosorbent assays (elisas) and immunoblotting. a crude soy extract and the 7s, 11s, and whey fractions were isolated from commercial defatted soy flakes. of 23 patients who underwent standardized soy challenges, seven were positive. the elisa results showed no statistically different re ...	2009	2709250
the major low-molecular-weight heat shock protein in chloroplasts shows antigenic conservation among diverse higher plant species.	several plant species are known to synthesize low-molecular-weight nucleus-encoded heat shock proteins (hsps) which localize to chloroplasts. dna sequence analysis of chloroplast hsp cdnas from pea (pisum sativum) and soybean (glycine max) has shown that the carboxyl-terminal halves of these proteins are homologous to low-molecular-weight hsps from a wide range of eucaryotes (e. vierling, r. t. nagao, a. e. derocher, and l. m. harris, embo j. 7:575-581, 1988). we used a pea cdna to construct fus ...	1989	2710111
community outbreaks of asthma associated with inhalation of soybean dust. toxicoepidemiological committee.	since 1981, 26 outbreaks of asthma have been detected in the city of barcelona. the geographic clustering of cases close to the harbor led us to consider the harbor as the probable source of the outbreaks. we therefore studied the association between the unloading of 26 products from ships in the harbor and outbreaks of asthma in 1985 and 1986. all 13 asthma-epidemic days in these two years coincided with the unloading of soybeans (lower 95 percent confidence limit of the risk ratio, 7.2). of th ...	1989	2710172
rgd-dependent linkage between plant cell wall and plasma membrane: consequences for growth.	soybean (glycine max [l.] merr. cv. mandarin) root cells (sb-1 cell line) grown in suspension culture containing glycyl-arginyl-glycyl-aspartyl-seryl-proline (grgdsp) (0.25 mg/ml), a synthetic peptide containing the rgd sequence found in many extracellular matrix adhesive proteins, demonstrated (a) significantly enhanced growth rate, and (b) aberrant cell wall/plasma membrane interactions and organization. substitution of the asp (d) by a glu (e) amino acid in the hexapeptide, or inversion of th ...	1989	2715184
characterization and solubilization of cyclo(his-pro) binding from rat liver membranes.	we have found cyclo(his-pro) binding in rat liver plasma membranes. this study focused on the characterization of solubilized binding for cyclo(his-pro) in rat liver membranes. the cyclo(his-pro) binding of liver membranes was solubilized by digitonin and octyl-glucopyranoside. the efficiency of solubilization with digitonin was greater. however, cyclo(his-pro) binding was not solubilized by triton x-100, chaps, or lubrol. digitonin-solubilized membranes showed cyclo(his-pro) binding with a high ...	1989	2726776
the preputial gland of the japanese serow capricornis crispus: ultrastructure and lectin histochemistry.	preputial glands of the japanese serow were found to consist of the modified sebaceous gland and apocrine gland in both sexes. in the modified sebaceous gland, cells transformed gradually toward the lumen from plentiful cytoplasm to a sponge-like network. lipid droplets were observed in mitochondria of differentiating cells. by lectin histochemistry, differentiating cells were stained with arachis hypogaea (pna), triticum vulgaris (wga) and canavalia ensiformis (con a) lectins, while secretory c ...	2009	2728845
soybean metal-binding proteins: calmodulin purification by hydrophobic interaction with polymer 3520.	polymer 3520, a non-polar styrene divinylbenzene polymer, provides a simple way to purify calmodulin (cam) from soybeans. this polymer, which selectively adsorbs cam by hydrophobic interaction within the polymer matrix, contains no exchangeable groups; thus, interaction with cam requires no ca++ ions, and elution is achieved with 50% ethanol. purification by this form of reversed-phase liquid chromatography is a substantial improvement over the conventional method, which requires high salt in el ...	2009	2740290
formation of highly ordered cross-linked lattices between asparagine-linked oligosaccharides and lectins observed by electron microscopy.	the interaction of asparagine-linked carbohydrates (n-linked) with carbohydrate binding proteins called lectins has been demonstrated to be involved in a variety of cellular recognition processes. certain n-linked carbohydrates have been shown to be multivalent and capable of binding, cross-linking, and precipitating lectins (bhattacharyya, l., ceccarini, c., lorenzoni, p., and brewer, c. f. (1987) j. biol. chem. 262, 1288-1293; bhattacharyya, l., haraldsson, m., and brewer, c. f. (1987) j. biol ...	1989	2745402
[nucleotide sequence coding glycinin of cultured soybeans glycine max and its wild ancestor glycine ussuriensis: clarification of the origin of cultured soy at the molecular level].		2006	2752902
demonstration of sugar residues in the ultimobranchial tubule and thyroid c-cells of the rat using peroxidase labelled lectins.	the distribution of various sugars, that contribute to the composition of the carbohydrate components of the glycoprotein in the ultimobranchial tubule and the thyroid c-cells has been studied, using eight peroxidase-labelled lectins (dolichos biblorus, glycine max, bauhinia purpurea, helix pomatia, arachis hipogaea, ulex europaeus i, limax flavus and sophora japonica). the results obtained seem to show that there are receptors for dolichos biflorus, glycine max, bauhinia purpurea, helix pomatia ...	1989	2757235
cytological effects of tc on young soybean plants.	soybean seedlings (glycine max cv. williams) were exposed for 24 to 67 h to 99tco4- (tc) at various concentrations in dilute culture solution. reduced primary leaf midrib length was observed with 67-h exposures to greater than or equal to 6.0 mu m tc. cellular effects were consistently observed by a light microscope after 43-h or longer exposure to 6.6 microm tc and higher concentrations. at lower tc levels, abnormal cells were interspersed among cells of normal appearance. abnormal cells displa ...	1989	2759869
ruminal metabolism in nonlactating cows fed whole cottonseed or extruded soybeans.	five nonlactating holstein cows (average weight 574 kg) with cannulas in the rumen and duodenum were fed five total mixed diets at 2.14% (dm) of bw for seven 2-wk periods to estimate the ruminal degradation of protein and fatty acids in whole cottonseed (wcs) and extruded soybeans (esb). lanthanum was used as an indigestible marker. ruminal propionic acid (molar proportion) was larger and butyric acid was smaller for wcs diets than for control or esb diets. total vfa (mm) and acetic acid (molar ...	1989	2768119
fate of aflatoxins in soybeans during the preparation of "soyogi".		1989	2770842
an immunologically related family of apolipoproteins associated with triacylglycerol storage in the cruciferae.	the major apolipoproteins associated with oil-storage bodies have been isolated from the mature seeds of six different species of the family cruciferae. the apolipoproteins were all of molecular mass 19-20 kda. they were highly abundant in mature seed tissue, accounting for up to 20% total seed proteins, and were localized exclusively on the membranes of oil-storage bodies. antibodies were raised in rabbits and mice against the six purified apolipoproteins. in each case, the antibodies specifica ...	1989	2774566
a rapid response to a plant hormone: auxin stimulates phospholipase a2 in vivo and in vitro.	addition of the active auxins indole-3-acetic acid, 2,4-dichlorophenoxyacetic acid or alpha-naphthylacetic acid to cultured soybean (glycine max l.) cells prelabeled with ethanolamine or choline increased the radioactivity in the lysophosphatidylethanolamine (lpe) or lysophosphatidylcholine (lpc) pool within 5 min. the inactive auxin analogue, beta-naphthylacetic acid, was inactive in this response. in membranes prelabeled in vivo, either with ethanolamine or choline, and subsequently isolated f ...	1989	2775252
changes in lectin binding of lumbar dorsal root ganglia neurons and peripheral axons after sciatic and spinal nerve injury in the rat.	the effects of chronic lesions of rat lumbar spinal or sciatic nerves on the binding of glycine max (soybean) agglutinin to galacto-conjugates, in small- and medium-size primary sensory neurons of the l4 and l5 dorsal root ganglia, were examined over a 580-day period. spinal nerve section resulted in a marked decrease in the population of stained neurons within 7 days. however, despite some retrograde morphological changes triggered by axonal injury, the proportion of stained nerve cells was nor ...	1989	2776185
a cacgtg motif of the antirrhinum majus chalcone synthase promoter is recognized by an evolutionarily conserved nuclear protein.	in the chalcone synthase gene of antirrhinum majus (snapdragon), 150 base pairs of the 5' flanking region contain cis-acting signals for uv light-induced expression. a nuclear factor, designated cg-1, specifically recognizes a hexameric motif with internal dyad symmetry, cacgtg, located within this light-responsive sequence. binding of cg-1 is influenced by c-methylation of the cpg dinucleotide in the recognition sequence. cg-1 is a factor found in a variety of dicotyledonous plant species inclu ...	1989	2780550
studies of lectin binding to the human cervix uteri: i. normal cervix.	lectins of bauhinia purpurea (bpa), canavalin ensiformis (con a), griffonia simplicifolia i (gs i), griffonia simplicifolia ii (gs ii), maclura pomifera (mpa), arachis hypogaea (pna), glycine max (sba), ulex europaeus i (uea i) and triticum vulgaris (wga) were used to evaluate cell surface carbohydrates in formalin-fixed paraffin-embedded tissue sections of normal human cervix uteri. consistent patterns of staining of the squamous epithelium were obtained in all 30 cases with bpa, gs ii, mpa, pn ...	1989	2793521
studies of lectin binding to the human cervix uteri: ii. cervical intraepithelial neoplasia and invasive squamous carcinoma.	the cell surface carbohydrate profile of formalin-fixed paraffin-embedded tissue sections of neoplastic cervical squamous epithelium was evaluated using lectins of bauhinia purpurea (bpa), canavalin ensiformis (con a), griffonia simplicifolia i (gs i), griffonia simplicifolia ii (gs ii), maclura pomifera (mpa), archis hypogaea (pna), glycine max (sba), ulex europaeus i (uea i) and triticum vulgaris (wga). three lectins (bpa, con a and pna) showed a similar pattern of staining in both normal squa ...	1989	2793522
bradyrhizobium japonicum glnb, a putative nitrogen-regulatory gene, is regulated by ntrc at tandem promoters.	the glnb gene from bradyrhizobium japonicum, the endosymbiont of soybeans (glycine max), was isolated and sequenced, and its expression was examined under various culture conditions and in soybean nodules. the b. japonicum glnb gene encodes a 12,237-dalton polypeptide that is highly homologous to the glnb gene products from klebsiella pneumoniae and escherichia coli. the gene is located directly upstream from glna (encoding glutamine synthetase), a linkage not observed in enteric bacteria. the g ...	1989	2793830
amine oxidase from lathyrus cicera and phaseolus vulgaris: purification and properties.	cu-amine oxidases (amine oxygen oxidoreductase deaminating, copper containing e.c. 1.4.3.6.) are found in all forms of life (1). they catalyze the following general reaction: r-ch2-nh2 + o2 + h2o----r-cho + nh3 + h2o2. cu-amine oxidases (cu-aos) have been extracted from different leguminosae: pisum sativum (2-3), lathyrus sativus (4), lens esculenta (5), vicia faba (6), cicer arietinum (7), glycine max (8) but not from phaseolus vulgaris. palavan and galston (9), in a study of polyamine biosynth ...	1989	2798365
soybean l-(+)-lactate dehydrogenases: purification, characterization, and resolution of subunit structure.	lactate dehydrogenase (ldh) (ec 1.1.1.27) from soybean (glycine max) was purified 2360-fold to homogeneity using ion-exchange, hydroxyapatite, affinity, and hydrophobic chromatographies. the molecular weight of the holoenzyme is 150,000 +/- 5000. two-dimensional (isoelectrofocusing-sodium dodecyl sulfate) gel electrophoresis reveals two polypeptides subunits of 5.9 and 6.5 pi and of 36,000 +/- 1000 and 37,000 +/- 1000 mr, respectively. nondissociating electrophoresis and isoelectric focusing of ...	1989	2802633
rhizobium japonicum usda 191 has two nodd genes that differ in primary structure and function.	several rhizobium genes (designated nod genes) are involved in early steps in nodule formation. here we present the results of dna sequence and functional analysis of two nodd genes from the symbiotic plasmid of usda 191, a fast-growing strain that forms nitrogen-fixing nodules on soybeans. both genes encoded full-length nodd-related polypeptides, which were 69% homologous to each other. one of these genes, nodd1, complemented a rhizobium trifolii nodd::tn5 mutant for clover nodulation; the othe ...	1988	2826389
two types of phosphorylase from etiolated soybean cotyledons.	two types of phosphorylase [ec 2.4.1.1] from the etiolated soybean (glycine max) cotyledons were separated by column chromatography on deae-sephacel and further purified to apparent homogeneities. molecular weights of the subunits were 100,000 and 113,000 for phosphorylases i and ii, respectively. the native enzymes i and ii were a dimer (200,000) and tetramer (450,000), respectively. electrophoretic analysis by the hedrick and smith method indicated that the two phosphorylases were distinct pro ...	1987	2828341
organization of the tgm family of transposable elements in soybean.	we have compared the organization of six tgm elements that were selected from a genomic library of soybean dna on the basis of hybridization with subcloned regions of tgm 1 (transposon, glycine max) from the seed lectin gene. these elements ranged in size from 1.6 kbp to greater than 12 kbp. tgm2, tgm3, tgm4 and tgm5 represent partial isolates in which the genomic clone contained a 3' but not a 5' terminus of the element; while tgm6 and tgm7, like tgm1, were small isolates flanked by both 5' and ...	1988	2848748
the primary structure of soybean (glycine max) ubiquitin is identical to other plant ubiquitins.		1988	2849766
cytochrome p-450-dependent monooxygenase activity in the velvetbean caterpillar, anticarsia gemmatalis hübner.	the activity of microsomal aldrin epoxidase was observed in various tissues of the last instar larva of the velvetbean caterpillar (anticarsia gemmatalis hübner) with the midgut being the most active. optimum conditions for assaying the enzyme were established. allelochemicals (monoterpenes, indoles and flavones) and host plants (cowpeas, cotton, peanuts, soybeans and hairy indigo) were found to induce the monooxygenase. piperonyl butoxide decreased and increased the toxicity of methomyl and met ...	1986	2869902
heterogeneity between soluble human and rabbit splenic alpha 2-adrenoceptors.	the pharmacological and biochemical characteristics of soluble alpha 2-adrenoceptors were investigated to determine whether differences observed in membranes were maintained in solution and to probe the nature of any such differences. alpha 2-adrenoceptors were solubilized from purified plasma membrane preparations of human and rabbit spleen using digitonin. [3h]yohimbine bound to one population of alpha 2-adrenoceptors in the preparations with dissociation constants of 2.4 nm and 7.8 nm respect ...	1986	2876709
role of the bradyrhizobium japonicum ntrc gene product in differential regulation of the glutamine synthetase ii gene (glnii).	we isolated the ntrc gene from bradyrhizobium japonicum, the endosymbiont of soybean (glycine max), and examined its role in regulating nitrogen assimilation. two independent ntrc mutants were constructed by gene replacement techniques. one mutant was unable to produce ntrc protein, while the other constitutively produced a stable, truncated ntrc protein. both ntrc mutants were unable to utilize potassium nitrate as a sole nitrogen source. in contrast to wild-type b. japonicum, the ntrc null mut ...	1988	2903856
oviposition habitats of psorophora columbiae in soybean fields of a texas riceland agroecosystem.	soil samples were collected over a 21/2-year period from microhabitats in fields planted to soybeans in chambers county, tx, to study the oviposition behavior of psorophora columbiae. an analysis of egg collection data gathered from the processing of 1,098 soil samples suggested that, during wet years, ps. columbiae eggs tend to be scattered throughout soybean fields. during dry years, a greater abundance of eggs occur in the low areas of these types of fields. the data also suggested that ovipo ...	1987	2904949
the effects of selected rice and soybean pesticides on the eggs of psorophora columbiae.	twenty-five pesticides used in the production of rice and soybeans in texas were tested in the laboratory to determine their toxicity to the eggs of psorophora columbiae. a reduction in hatching rate occurred when eggs were treated with a herbicide formulation containing thiobencarb and with one containing a tank mixture of propanil and molinate. a carbaryl formulation induced hatching of eggs prior to their exposure to the hatching stimulus. reduced survival to second instar of larvae hatching ...	1985	2906685
the accuracy of some simple models for predicting particulate interception and retention in agricultural systems.	the accuracy of three radionuclide transfer models for predicting the interception and retention of airborne particles by agricultural crops was tested using pu-bearing aerosols released to the atmosphere from nuclear fuel facilities on the u.s. department of energy's savannah river plant, near aiken, sc. the models evaluated were: 1) nrc, the model defined in u.s. nuclear regulatory guide 1.109; 2) food, a model similar to the nrc model that also predicts concentrations in grains; and 3) agns, ...	1989	2925383
effect of supplemental niacin or niacinamide and soybean source on ruminal bacterial fermentation in continuous culture.	effects of niacin or niacinamide in diets containing either soybean meal, raw whole soybeans or whole soybeans extruded at 132 and 149 c on ruminal bacterial fermentation were examined with a dual-flow continuous culture system. in exp. 1, soybean sources each provided 50% of total crude protein in diets comprised of 52% concentrate mix, 36% corn silage and 12% alfalfa hay (dry-matter basis). each diet was supplemented with 0 or 100 mg/kg niacin. niacin supplementation increased (p less than .05 ...	1985	2934360
effects of low temperature on the respiratory metabolism of carbohydrates by plants.	the effects of lowering the temperature from 25 degrees c to 2-8 degrees c on carbohydrate metabolism by plant cells are considered. particular emphasis is placed on the mechanism of cold-induced sweetening in tubers of potato (solanum tuberosum). temperatures between 0 and 10 degrees c were shown to cause a marked reduction in the rate of respiration of a wide range of plant tissues. at these temperatures the ability of suspension cultures of soybean (glycine max), and callus cultures and tuber ...	1988	2978665
identification of a nodd-dependent locus in the rhizobium strain ngr234 activated by phenolic factors secreted by soybeans and other legumes.	transfer of the strain ngr234nodd 1 gene into the narrow host range r. trifolii strain anu843 on either a 6.7-kb hindiii or 17-kb xhoi fragment broadens the host range of this bacterium to include the tropical legumes vigna unguiculata, glycine ussuriensis, leucaena leucocephala, and siratro (macroptilium atropurpureum). contrary to previous data (bassam et al. 1986), mutagenesis of the 17-kb xhoi fragment with a mini-mu lac transposon (mu dii1734) showed that a functional nodd 1 gene was essent ...	1988	2980201
slow-growing rhizobium japonicum comprises two highly divergent symbiotic types.	we examined the interrelationships of the genomes of 10 slow-growing strains of rhizobium japonicum to provide a foundation for molecular genetic studies of these agriculturally important endosymbiotic bacteria of commercial soybeans. the degree of base substitution in and around known symbiotic genes (nif and presumptive nod), constitutively expressed genes (glna and reca), and two other cloned sequences was estimated from restriction site variation by using cloned dnas as hybridization probes ...	1985	2989244
expression of symbiotic genes of rhizobium japonicum usda 191 in other rhizobia.	a 200-megadalton plasmid was mobilized from rhizobium japonicum usda 191 to other rhizobium strains either that cannot nodulate soybeans or that form fix- nodules on certain cultivars. the symbiotic properties of the transconjugants indicate that both soybean specificity for nodulation and cultivar specificity for nitrogen fixation are plasmid encoded.	1985	2989250
proton motive force in washed cells of rhizobium japonicum and bacteroids from glycine max.	the components of the proton motive force (delta p), namely the membrane potential and the transmembrane ph gradient, were measured in washed cells of rhizobium japonicum cc705 grown in cultures (5% o2-95% n2) in the presence of 10 mm kno3 and in bacteroids from glycine max. the delta p and its components remained reasonably constant in cells as well as in bacteroids at various stages of growth. the effects of uncouplers and atpase inhibitors on the delta p and its components were determined in ...	1985	2999086
comparison of the effect of cell wall and hull fiber from canola and soybean on the bioavailability for rats of minerals, protein and lipid.	hull or cell wall material, isolated from canola (brassica napus cv. regent) or soybean (glycine max) or cellulose was added to a basal, semipurified diet at a level of 12% and fed to growing male wistar rats. the apparent availability of cu, fe, ca, p and protein were lower when the fiber-containing diets were fed compared to the control diet. the availability of mg was lower when the canola hull (ch) and cellulose (ce) diets were fed, whereas zn availability was lower when the canola cell wall ...	1986	3003294
lectin binding to elastic fibres and associated components during development of the human aorta.	a panel of peroxidase-conjugated lectins was used to stain frozen sections of human foetal, neonatal, infant and adult aortae. two main distinctive staining patterns were observed at the light microscope level, depending on the lectin employed. lectins from concanavalin a, triticum vulgaris, arachis hypogaea and glycine max resulted in staining of the surfaces of aortic elastic lamellae and the interlamellar areas. with lectins from dolichos biflorus and bandeiraea simplicifolia staining was see ...	1985	3009360
the glycosylated seed storage proteins of glycine max and phaseolus vulgaris. structural homologies of genes and proteins.	considerable information is now available concerning the 7 s seed storage proteins of legumes and the genes that encode them. our study compares the gene encoding a beta-type subunit of phaseolin (pvu beta), the 7 s protein of common bean (phaseolus vulgaris), with the gene encoding an alpha'-subunit of beta-conglycinin (gma alpha'), the 7 s protein of soybean (glycine max). the comparison involves 2880 base pairs of pvu beta and 3636 base pairs of gma alpha' and includes approximately 1 kilobas ...	1986	3013879
isolation of competition-defective mutants of rhizobium fredii.	we coupled tn5 mutagenesis with a competition assay to isolate mutants of rhizobium fredii usda 257 that are defective in competition for nodulation of soybeans. two mutants with single tn5 inserts in the chromosome showed reduced competitiveness in vermiculite but were identical to the wild-type strain in symbiotic properties when inoculated alone. recombination of tn5 and flanking genomic regions cloned from the mutants into the parent strain showed that tn5 was responsible for the mutant phen ...	1987	3025184
[rna-recognizing function of the dna-recognizing structure helix-turn-helix in ribosomal proteins s4 and s7 of escherichia coli and plant chloroplasts].	the segments which satisfy the necessary requirements for dna-recognizing structure helix-turn-helix coding have been found in amino acid sequences of rna-recognizing e. coli ribosomal proteins s4 and s7. it has appeared that among these segments there are those that in chloroplasts s4 and s7 homologues satisfy these necessary requirements also: s4-97-121 and 108-132 (ec), 91-115 and 102-126 (zea mays); s7-109-133 (ec) 110-134 (euglena gracilis, glycine max). such segments have been confronted w ...	1988	3054493