Publications

identification of glycoprotein storage diseases by lectins: a new diagnostic method.	the specific diagnosis of glycoprotein storage diseases is made by demonstrating a deficiency in enzyme activity or an elevation of undegraded oligosaccharides in cells or body fluids. prospective sampling and expensive specialized biochemistry, which is also time consuming, are required for such studies. we used lectin reagents on paraffin-embedded tissue sections to identify the specific sugars in undegraded stored substances. we studied 22 cases of glycoprotein storage diseases and differenti ...	1984	6501863
[effect of formaldehyde on the protection of proteins in the silage of the peanut plant (arachis hypogaea)].	an experiment was performed to study the formaldehyde protective effect on the ensilaged whole peanut plant protein (21.5% cp). the experimental design consisted in four treatments 5, 10, 15 and 20% formaldehyde, which was added in the proportion of 5 lt, per ton. a control group without any formaldehyde was also included. there were no significant differences in regard to ph among treatments (5.56 to 5.70). ammonia concentration dropped significantly in all treatments, a finding which suggests ...	1984	6545651
lectin-binding components of normal granulocytes and leukaemic myeloid cells.	a panel of lectins was used to analyse glycoproteins of normal granulocytes and leukaemic myeloid cells. the glycoproteins of detergent-solubilized whole cells were separated by sodium dodecyl sulphate/polyacrylamide-gel electrophoresis and their lectin-binding properties determined by incubation of the fixed gels with radioiodinated lectins. normal granulocytes and leukaemic myeloid cells in different stages of maturation possess a cell-surface sialic acid-rich glycoprotein of apparent mol.wt. ...	1983	6577859
selective binding of peanut lectin by t lineage lymphocytes in paraffin sections.	on sections of formalin fixed paraffin embedded lymph nodes, thymuses and spleens, binding of the lectin from peanut (arachis hypogaea) was detected on some histiocytes, while the lymphoid cells were negative. after treatment of the sections with neuraminidase, sialic acid covered beta-d-galactosyl (1-3)-n-acetyl-d-galactosamine residues were revealed in a more extensive number of histiocytic cells and on a sub-population of lymphoid cells with the typical distribution of t lineage lymphocytes. ...	1983	6605747
flow cytometric analysis of the binding of eleven lectins to human t- and b-cells and to human t- and b-cell lines.	the relative surface binding of 11 lectins to human peripheral blood t- and b-lymphocytes, to molt-4 and jm t-cell lines, and to 6410 and nc37 b-cell lines was determined by flow cytometry. the lectins from lens culinaris (lca), ricinus communis (rca), arachis hypogaea (pna), abrus precatorius (apa), ulex europaeus (uea-f), sarothamnus scoparius (sas-f), helix pomatia (hpa), phaseolus coccineus (l-pha), glycine max (sba), and triticum vulgare (wga) were fluoresceinated and incubated with living, ...	1984	6609053
purification of peanut (arachis hypogaea) agglutinin isolectins by chromatofocusing.	this report describes the purification and isolation of the isolectins of peanut agglutinin (pna). we find the affinity-purified pna to have an amino acid composition similar to that reported by lotan et al. (j. biol. chem. 250, 8518-8523 (1975)); however, our preparation contains three tryptophan residues per subunit rather than two as reported by lotan et al. this higher tryptophan content is consistent with the extinction coefficient (e1 mg/ml 280) of 0.96 as previously reported (fish et al., ...	1983	6614478
studies on the possible chemical, immunochemical and morphological differences at the cell surfaces of immunosensitive and immunoresistant, moloney virus-induced, lymphoma cell-lines.	comparison was made of several cell-surface parameters in the immunosensitive, moloney virus-induced, mouse lymphoma, yac, and its immunoresistant variant, yacir. the characteristics of the two cell lines appeared to be similar by most of the criteria employed. the poly(acrylamide)-gel electrophoresis (with sodium dodecyl sulfate) patterns, after staining with coomassie brilliant blue, of detergent-solubilized materials, appeared to be identical. after elution from a gel filtration column, no ma ...	1983	6627250
amino acid sequences of trypsin-chymotrypsin inhibitors (a-i, a-ii, b-i, and b-ii) from peanut (arachis hypogaea): a discussion on the molecular evolution of legume bowman-birk type inhibitors.	the amino acid sequences of four peanut protease inhibitors (a-i, a-ii, b-i, and b-ii) were determined by conventional methods and by comparison of peptide maps of their tryptic digests with that of b-iii on hplc. a-i, a-ii, b-i, and b-iii had the same amino acid sequence except for differences in their n-terminal regions. this suggests that the four inhibitors would be derived from an original inhibitor with a longer n-terminal amino acid sequence by proteolysis of its n-terminal region. but b- ...	1983	6630176
purification & properties of arginase from arachis hypogea l. seedlings.		1983	6671687
hemolytic-uremic syndrome and t-cryptantigen exposure by neuraminidase-producing pneumococci: an emerging problem?	hemolytic anemia, thrombocytopenia, and renal failure form a constellation of complications observed in patients infected with neuraminidase-producing pneumococci. the circulating enzyme causes exposure of the t-cryptantigen on cell surfaces to which most people possess a naturally occurring antibody. antigen-antibody interaction activates effector systems that lead to the clinical manifestations. the syndrome is most frequently seen in infants, in whom it carries more than a 50% mortality rate. ...	1983	6687290
lectin histochemistry of 1,2-dimethylhydrazine-induced rat colon neoplasia.	lectins linked to fluorescein were used as carbohydrate probes to examine the goblet cell mucin and epithelial cell surface glycoconjugate alterations in an experimental rodent model of colonic neoplasia induced with parenteral 1,2-dimethylhydrazine dihydrochloride. lectins derived from triticum vulgare (wga), ricinus communis (rca1), and limulus polyphemus (lpa) showed reduced labeling of goblet cell mucin in these tumors, while binding with peanut lectin from arachis hypogaea (pna), a lectin o ...	1983	6688437
carbohydrate chain analysis by lectin binding to electrophoretically separated glycoproteins from murine b16 melanoma sublines of various metastatic properties.	cellular glycoprotein carbohydrate chains of b16 melanoma sublines of various metastatic colonization capacities were analyzed by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate and direct lectin staining, combined with chemical modification of carbohydrate chains in situ. for these studies, we utilized b16 sublines selected for low (b16-f1) or high lung (b16-f10), high brain (b16-b15b), or high ovary (b16-o13) colonization properties, or high tissue invasiveness in ...	1984	6692378
differential binding characteristics and applications of dgal beta 1----3dgalnac specific lectins.	the binding properties of arachis hypogaea (pna), bauhinia purpurea alba (bpl), maclura pomifera ( mpl ) and sophora japonica (sjl) lectins were studied by quantitative precipitin and precipitin inhibition assays, demonstrating them to be most specific for dgal beta 1---- 3dgalnac residues. additionally, each lectin had its own binding characteristic such as different binding activities to dgal beta 1---- 4dglcnac or dgal beta 1---- 3dglcnac beta 1----linked oligosaccharides, and/or dgalnac alph ...	1984	6727869
lectins as markers of human epidermal cell differentiation.	the expression of sugar residues on human epidermal cells was investigated by means of lectin binding, as a way of determining membrane structural changes occurring during the differentiation of the epidermis. fourteen lectins of different sugar specificity were conjugated with fluorescein isothiocyanate (fitc-lectins) and tested in fluorescence microscopy on frozen sections of normal human epidermis. in parallel, fitc-lectins were tested on psoriatic-involved epidermis to visualize differences ...	1982	6816653
histochemical studies on lectin binding in reactive lymphoid tissues.	using the avidin-biotin-labeled peroxidase complex (abc) method, the staining reaction of a panel of 12 biotin-labeled lectins was studied in formalin-fixed, paraffin-embedded reactive lymph nodes and tonsils. varying degrees of lectin binding were observed in lymphoid cells and macrophage-histiocytes with concanavalin ensiformis (con a), lens culinaris (lca), phaseolus vulgaris (pha), pisum sativum (psa), ricinus communis (rca), and triticum vulgaris (wga) agglutinins, but no evidence of bindin ...	1983	6827084
amino acid sequence of a trypsin-chymotrypsin inhibitor, b-iii, of peanut (arachis hypogaea).	the amino acid sequence of peanut trypsin-chymotrypsin inhibitor, b-iii, was determined by conventional methods. the limited proteolysis of b-iii with trypsin indicated the reactive sites of b-iii for trypsin to be arg(10)-arg(11) and arg(38)-ser(39). comparison of the established sequence of b-iii with those of other bowman-birk type double-headed protease inhibitors indicated that b-iii has four amino acid insertions and one amino acid deletion. it is especially interesting that the amino acid ...	1983	6841347
purification and properties of a 4-methylene-l-glutamine amidohydrolase from peanut leaves.	a 4-methylene-l-glutamine amidohydrolase has been partially purified from leaf extracts of 2-week germinated peanuts (arachis hypogaea). purification steps include deae-sephacel and gel filtration chromatography as well as chromatofocusing; amidohydrolase purified 300- to 400-fold is obtained. the enzyme has an approximate molecular weight of 45,000 as determined by gel filtration chromatography and polyacrylamide gel electrophoresis, a broad activity optimum between ph 8.0 and 9.0, and is highl ...	1983	6863306
binding of fitc-coupled peanut-agglutinin (fitc-pna) to embryonic chicken retinas reveals developmental spatio-temporal patterns.	fitc-labeled peanut agglutinin (pna), a lectin from peanut (arachis hypogaea) which binds specifically to d-galactose, was used as a probe to trace the distribution of pna-lectin receptors during early development of chick retina. we found that specific fluorescence within the inner plexiform layer (ipl) and the outer plexiform layer (opl) appears at different developmental stages. the specific fluorescence of the ipl first appears near the central area of the ventro-posterior quadrant of the 7- ...	1983	6871724
production of bacillus sphaericus strain 1593 primary powder on media made from locally obtainable nigerian agricultural products.	five media, formulated from dried cow blood, mineral salts, and seeds from four species of legumes, were assessed for growth, sporulation, and insecticidal properties of bacillus sphaericus strain 1593. bacterial powders, prepared from broth, were assayed against culex quinquefasciatus, anopheles gambiae, and aedes aegypti. good growth and sporulation were obtained with all the media. the highest number of viable cells and spores per mililitre (8.6 x 10(8) and 8.1 x 10(8] were obtained in media ...	1983	6883226
binding of lectins to leukemic cell lines.	the binding of 12 different fluorescein-conjugated lectins to 10 all (acute lymphoblastic leukemia) cell lines and two cell lines derived from patients in blast crisis of myeloid leukemia was examined. the specificity of the membrane fluorescence was demonstrated by inhibition with various saccharides. three of the lectins bound to all cell lines, four bound to only some of the lines, and five were not bound. there was no correlation between the binding pattern and the immunological phenotype of ...	1980	6928109
alterations in human colonic mucin occurring with cellular differentiation and malignant transformation.	the binding of fluorescein isothiocyanate (fitc)-conjugated lectins to mucin in the human colon was studied by using fluorescence microscopy. in normal mucosa, lectins that preferentially bind to exposed n-acetyl-galactosamine residues (dolichos biflorus agglutinin and soybean agglutinin) bound selectively to the goblet cell mucin of well-differentiated cells in the upper colonic crypt. by contrast, lectins that require exposed non-reducing galactose residues for binding (ricinus communis agglut ...	1982	6952252
the major sialoglycoprotein of human t-lymphocytes.	human, peripheral-blood t-lymphocytes and human, t-lymphoblastoid cells of a molt 4b cell-line were surface-labeled by lactoperoxidase-catalyzed iodination, periodate and sodium borotritide, and galactose oxidase and sodium borotritide, and analyzed by dodecyl sodium sulfate-polyacrylamide gel-electrophoresis. both types of cells were found to show a major, cell-surface sialoglycoprotein with an apparent mol. wt. of 95,000. after neuraminidase treatment, this glycoprotein showed a higher mol. wt ...	1980	6965465
lymphocyte cell surface glycoproteins which bind to soybean and peanut lectins.	in cellular immunology, peanut (arachis hypogaea) lectin has been used to selectively agglutinate immature lymphoid cells and soybean (glycine max-lectin to agglutinate b lymphocytes. we have used affinity chromatography to study the surface glycoproteins of rat and mouse lymphoid cells which bind to these lectins. thymocyte and t and b lymphocyte glycoproteins were analysed either without modification (native) or after the removal of sialic acid with neuraminidase (asialo). the only native glyc ...	1982	6980823
the role of lectins in blood group serology.	many lectins display blood group activity, and extracts from dolichos biflorus (anti-a1), ulex europaeus (anti-h), and vicia graminea (anti-n) seeds provide an alternative to human sera as a source of blood-typing reagents. however, the major application of lectins in blood group serology undoubtedly lies in the recognition and elucidation of red celll polyagglutination. in this respect, lectins from arachis hypogaea (anti-t/tk), salvia sclarea (anti-tn). salvia horminum (anti-tn + cad). dolicho ...	1980	6998652
extent of desialation of blood group mm, nn, and mn antigens required for reactivity with human anti-t antibody and arachis hypogaea lectin.	immunochemical activity of the arachis hypogaea lectin has been equated, in spite of its different hapten combining requirements, with that of the human anti-t (thomsen-friedenreich) antibody population which is of importance in cancer immunology. we show here by gradual desialation of isolated human blood group mm and nn antigens at ph 2.0, 56 degrees c, that a. hypogaea lectin is, in addition, substantially more tolerant to neuac in the vicinity of t antigen's immunodominant repeating structur ...	1982	7061447
translocation, accumulation and persistence of carbofuran in paddy, ground nut, and cotton.		1982	7066567
involvement of a tyrosyl residue in the interaction of peanut lectin with lactose.	the role of a tyrosyl residue in the binding of arachis hypogaea (peanut) agglutinin, aha, to lactose has been studied using two techniques, titration of the phenolic hydroxyl group of the tyrosine residue and chemical modification of the tyrosine with iodine. more than three tyrosyl residues per mol of aha were masked when aha was titrated in the presence of lactose. lactose also protected some tyrosyl residues of aha from the modification with iodine. upon interaction with lactose, aha iodinat ...	1982	7076653
crystallization and preliminary x-ray studies of the anti-t lectin from peanut (arachis hypogaea).		1982	7077661
purification and characterization of protease inhibitors from peanuts (arachis hypogaea).	five protease inhibitors were isolated from peanut seeds and named a-i, a-ii, b-i, b-ii, and b-iii. these inhibitors seemed to be bowman-birk type inhibitors judging from their low molecular weights and high cystine contents. all the inhibitors inhibited both bovine trypsin and chymotrypsin at ratios of 1:2 and 1:1, respectively, but not simultaneously. the complexes of the inhibitors and trypsin no longer inhibit chymotrypsin. on the other hand, their complexes with chymotrypsin inhibit trypsin ...	1982	7096296
acquired b antigen and polyagglutination in a apparently healthy blood donor.	this work presents the second published report of an apparently healthy adult with an acquired b antigen. this case is unique because of the serial studies documenting the increase in strength of the b antigen, development of polyagglutinability, and absence of detectable gastrointestinal pathology. the cells of g.r., a 56-year-old caucasian female, came to our attention because of weak reactions with anti-b. her cells were typed normal group a one year earlier. g.r.'s cells specifically absorbe ...	1982	7100751
[effect of a diet supplemented with unsaturated fatty acids (arachis hypogaea: peanuts) on blood coagulation].		1982	7122948
ultraviolet difference spectroscopy study of peanut lectin binding to mono- and disaccharides.	ultraviolet difference spectroscopy was used to study the interaction of peanut (arachis hypogaea) lectin with complementary carbohydrates. a correlation was observed between variations of ultraviolet spectra during the binding of sugars to the lectin and the specificity and the strength of the binding. the association constant, free energy, enthalpy and entropy for peanut lectin-lactose interactions were calculated over the temperature range 10-30 degrees c. the binding constants for 10 mono- a ...	1982	7126638
circular dichroism studies on conformational transitions of phytohemagglutinins effected by some alcohols.	the effects of methanol, ethanol, 1-propanol and 2,2,2,-trifluoroethanol (tfe) on conformation of 10 phytohemagglutinins (lectins) were examined by applying the circular dichroism (cd) method. concanavalin a and the lectins isolated from pea (pisum sativum), lentil (lens culinaris), soybean (glycine max), peanut (arachis hypogaea), dolichos biflorus, sophora japonica, lotus tetragonolobus, ulex europaeus i, and phaseolus vulgaris (leucoagglutinin) were used. slight changes in the structure of th ...	1982	7150429
the surface glycoproteins of human skin fibroblasts detected after electrophoresis by the binding of peanut (arachis hypogaea) agglutinin and ricinus communis (castor-bean) agglutinin i.	a new methodology was developed to study the cell-surface glycoproteins of cultured human skin fibroblasts. this was based on the binding of a variety of biotinyl-lectins to nitrocellulose electrophoretic transfers of total fibroblast lysates after separation in sodium dodecyl sulphate/polyacrylamide gels, followed by reaction with avidin-biotinyl-peroxidase complexes and detection with 3,3'-diaminobenzidine. the technique proved to be very sensitive and a large number of glycoproteins were dete ...	1982	7159403
receptors for peanut agglutinin in lymphocytes from patients with cirrhosis of the liver.	a population of peripheral blood lymphocytes exhibits specific receptor for the peanut lectin (arachis hypogaea). in control subjects, this population represents 1.3% of all lymphocytes while it represents 13% in patients with alcoholic cirrhosis. the percentage of lymphocytes expressing this receptor appears to be correlated with hypoalbuminemia and the erythrocyte sedimentation rate, in these patients. furthermore, a higher percentage of peanut positive lymphocytes was found in cirrhotic patie ...	1982	7183347
interaction of lectins from soybean and peanut with rhizobia that nodulate soybean, peanut, or both plants.	four of 14 strains of rhizobium japonicum from soybean nodulated peanut (arachis hypogaea l. cultivar jumbo virginia), and 3 of 8 rhizobium sp. strains from peanut nodulate soybean (glycine max (l.) merr. cultivar harosoy 63). cells of three peanut rhizobia bound fluorescent- and radioisotope-labeled soybean lectin. two of these strains failed to nodulate soybean, and conversely, two peanut strains that nodulated soybean did not bind to soybean lectin. both culture medium and age had pronounced ...	1980	7195297
simple test for detection of virus neuraminidase and antineuraminidase using lectins (lectin-neuraminidase test system).	the fast and sensitive detection of virus neuraminidase effectiveness (influenza virus a and b or mumps virus) and of antibodies against virus neuraminidase in human serum is described. lectin mainly obtained from arachis hypogaea and helix pomatia is used in the lectin-neuraminidase test (ln-test). the lectins are capable of agglutinating erythrocytes after virus incubation because virus neuraminidase reveals the t-antigen to be situated on erythrocytes. the presence of anti-neuraminidase in hu ...	1980	7223125
identification of methionine-rich polypeptides in peanut (arachis hypogaea l.) seed.		2015	7229215
[neuraminidase-producing pneumococci in the pathogenesis of hemolytic-uremic syndrome].	hemolytic-uremic syndrome (hus) accompanied by pneumococcal infections forms a characteristic subgroup of hus. pneumococcal neuraminidase splits off neuraminic acid from the glycoproteins present on the surface of red cells, thrombocytes and endothelial cells, and thus exposes the hidden thomsen cryptantigen (t-ag). the t-ag can then react with a complement-fixing antibody of the igm class which is present in all human plasmas after the age of 6 months. early diagnosis of t-transformation should ...	1980	7280595
salinity induced changes in the activities of aspartate & alanine amino transferases & glutamate dehydrogenase in peanut (arachis hypogaea l.) leaves.		1981	7309162
[experiments to elucidate the role of aspergillus fungi in the hemorrhagic syndrome in poultry].	attempts were made to assess the role played by fungi of the aspergillus genus in the occurrence of the hemorrhagic syndrome in birds. groups of test birds were given continuously a forage mixture containing 30 per cent of ground nut cake which had aflatoxin at the rate of 1.5 ppm, a forage mixture 30 per cent of which was preliminarily contaminated with spores of asp. fumigatus or asp. flavus, and subtoxic amounts of nonpurified aflatoxin. growth retardation was established along with the devel ...	1981	7331224
peanut lectin binding properties of germinal centres of mouse lymphoid tissue.	peanut (arachis hypogaea) lectin (pnl) has been shown to agglutinate the 90% of cells from murine thymus which are supposed to be immature cortical thymocytes. further studies on the numbers of thymocytes binding fluorescein isothiocyanate conjugated pnl (fitc-pnl) confirmed the large proportion of pnl binding cells. in other organs such as bone marrow, spleen and peripheral lymph nodes, smaller proportions of pnl positive cells have been recorded. pnl-positive cells outside the thymus have been ...	1980	7360273
evidence of accumulation of ceramides containing [14c]nervonic acid in the rat lung following injection of [14c]erucic acid.	a mixture of albumin-bound [14c]erucate and [3h]oleate was injected into rats fed a stock pellet diet containing 4% by weight of lipid. chylomicrons containing the same labelled fatty acids were also injected into rats fed diets containing 15% by weight of rapeseed oil (48% of erucic acid), canbra oil (less than 5% of erucic acid) or ground nut oil (no erucic acid). lung lipids were analyzed at various times after injection. in all cases, except in the rapeseed oil diet group, 14c radioactivity ...	1980	7370286
detection of lectin-sugar interaction by ultraviolet difference spectroscopy.	it was found that the addition of specific sugars to solutions of several lectins induced ultraviolet difference spectra. the difference spectra of lectins from lens culinaris, sophora japonica, solanum tuberosum and wheat germ have two peaks at 292 nm and 284-287 nm which are characteristic of the tryptophanyl residue. the difference spectrum of arachis hypogaea agglutinin has two peaks at 285 nm and 279 nm which seem to be characteristic of the tyrosyl residue. in addition to the identificatio ...	1980	7451408
lectin binding sites in the choroid plexus and choroid plexus papillomas.	the surface of the cells of the normal choroid plexus and of the plexus papillomas is coated by sialomucopolysaccharides, containing substances which are positively stained with the colloidal iron (hale-) reaction. after pretreatment with neuraminidase sialic acid is removed rendering the membrane negative to the hale reaction. using fitc- or rhodamine-labelled pna (arachis hypogaea) and rca (rhicinus communis) lectins specific receptors are demonstrable. the identity of these distinct oligosacc ...	1980	7453965
preneoplastic and neoplastic lesions of rat hereditary renal cell tumors express markers of proximal and distal nephron.	long-evans (eker) rats carry a mutation that predisposes them to develop spontaneous renal cell tumors of two morphologic patterns: solid chromophilic masses or cystic lesions lined by eosinophilic cells. previous studies have suggested that these tumors arise from the proximal tubules. in the present study, lectin-binding characteristics and cytokeratin expression of various stages of hereditary rat renal epithelial neoplasia were examined to localize the portion of the nephron from which tumor ...	1995	7483212
intermediate filament expression and lectin histochemical features of canine transmissible venereal tumour.	immunocharacterization of intermediate filament proteins and lectin-binding studies were carried out in canine transmissible venereal tumour (tvt), a unique neoplasia sharing some epidemiological features with kaposi's sarcoma in humans. neoplastic cells express vimentin, but neither cytokeratin nor desmin. regarding lectins, tvt cells express receptors for triticum vulgaris (wga), concanavalia ensiformis (con a) and ricinus communis i (rca-i). they appear to be negative for ulex europaeus-i (ue ...	1995	7488385
echinostoma caproni and e. trivolvis alter the binding of glycoconjugates in the intestinal mucosa of c3h mice as determined by lectin histochemistry.	mouse (c3h) mucosal glycoconjugates were examined in normal small intestines and intestines infected with echinostoma caproni or e. trivolvis using six different fluorescein-conjugated lectins: triticum vulgaris agglutinin (wga), ulex europaeus agglutinin i (uea-i), ricinus communis agglutinin i (rca-i), glycine max soybean agglutinin (sba), dolichos biflorus agglutinin (dba), and arachis hypogaea peanut agglutinin (pna). the expression of lectin-binding sites and the intensity of the binding of ...	1993	7507137
lectin binding and bcl-2 protein expression in craniopharyngiomas.	the maturation process of basal cells in craniopharyngiomas was studied using a panel of lectins, and antibodies against cytokeratin 13 and bcl-2 protein, using oral mucosa for comparison. seven lectins were employed: peanut (arachis hypogaea) agglutinin, dolichos biflorus agglutinin (dba), ulex europaeus agglutinin-i (uea-i), soybean agglutinin, ricinus communis agglutinin-i, succinyl wheat germ agglutinin, and pisum sativum agglutinin. dba and cytokeratin 13 stainings of the suprabasal cells i ...	1994	7526231
porcine mitogen-induced interferon.	induction of porcine interferon (poifn) with different lectins (phytohaemagglutinin, lens culinaris lectin, arachis hypogea lectin) in various concentrations (500, 50 and 5 micrograms/ml) was studied. kinetics of poifn appearance was analyzed by antiviral and antiproliferative tests in human transformed and nontransformed cells. the relation between antiviral and antiproliferative activity of poifn in terms of respective units was determined. poifn was characterized in comparison with human inte ...	1994	7526670
lectin binding distinguishes between neuroendocrine and neuronal derivatives of the sympathoadrenal neural crest.	lectin cytochemistry was used to identify surface epitopes selectively expressed by chromaffin cell chemoreceptors (glomus cells) in the rat carotid body. unexpectedly, these studies revealed that binding sites for peanut agglutinin (pna; arachis hypogea) were highly expressed by all neuroendocrine-derivatives of the sympathoadrenal neural crest, including glomus cells, small, intensely fluorescent cells, and adrenal chromaffin cells in situ. in contrast, principal sympathetic neurons did not ex ...	1995	7535839
genetic and phenetic analyses of bradyrhizobium strains nodulating peanut (arachis hypogaea l.) roots.	seventeen bradyrhizobium sp. strains and one azorhizobium strain were compared on the basis of five genetic and phenetic features: (i) partial sequence analyses of the 16s rrna gene (rdna), (ii) randomly amplified dna polymorphisms (rapd) using three oligonucleotide primers, (iii) total cellular protein profiles, (iv) utilization of 21 aliphatic and 22 aromatic substrates, and (v) intrinsic resistances to seven antibiotics. partial 16s rdna analysis revealed the presence of only two rdna homolog ...	1995	7538280
localization of peanut (arachis hypogaea) root lectin (pra ii) on root surface and its biological significance.	the glucose-specific peanut root lectin, pra ii, is localized on the surface of 7-day-old peanut seedling root and in root cortical parenchymatous cells. the lectin is eluted from intact roots upon washing with buffer containing glucose. rabbit erythrocytes bind to the root surface and the cortical cells; the binding is inhibited by antibodies raised against pra ii, peanut-specific rhizobium cells and by glucose. lipopolysaccharides isolated from host-specific rhizobium strain inhibit the haemag ...	1995	7540901
purification and characterization of an acid phosphatase from arachis hypogaea.	an acid phosphatase from arachis hypogaea (peanuts) has been purified. the electrophoretically homogeneous enzyme preparation is free of any phophodiesterase activity. the enzyme has a molecular weight of 120,000. among the various phosphomonoesters tested, p-nitrophenylphosphate was found to be its most effective substrate. the km for p-nitrophenylphosphate was 1.21 mm at ph 5.0 and 25 degrees c. the enzyme was thermostable and did not loose activity after 1 hr at 50 degrees c.	1995	7549937
[h2o2 generation and human neutrophil aggregation as affected by lectins].	the effects of eight plant lectins on human neutrophils aggregation and h2o2 release were studied. both processes were stimulated by lectins from viscum album, triticum vulgaris, phaseolus vulgaris and canavalia ensiformis while lectins from solanum tuberosum, sambucus nigra and glycine mas displayed only aggregating activity and arachis hypogaea lectin was not effective. heating (46 degrees c) and uv-radiation during 4-5 min were found to suppress completely h2o2 release from neutrophils keepin ...	1995	7557235
purification and partial characterization of acyl carrier proteins from developing oil seeds of pisa (actinodaphne hookeri) and ground nut (arachis hypogaea).	acyl carrier proteins (acp) were purified to homogeneity in the active form from developing seeds of pisa (actinodaphne hookeri) which synthesizes exclusively trilaurin and from ground nut (arachis hypogaea) which synthesizes triacylglycerols containing long chain fatty acids. two major isoforms of acps were purified from developing pisa seeds using deae-cellulose, superose-6 fplc and c4 reversed phase hplc chromatographic methods. in contrast, only a single form of acp was present in ground nut ...	1995	7590854
glycoconjugate expression of chondrocytes and perichondrium during hyaline cartilage development in the rat.	alterations in the expression of glycoconjugate structures during cartilage development in the chondrocranium, nasal skeleton, meckel's cartilage, limb buds, vertebral bodies and ribs were investigated comparatively in 13 to 21-d-old rat embryos. the binding patterns of 24 biotinylated lectins were analysed in serial sections and compared with results obtained using histochemical methods. proteoglycan distribution, assessed by conventional staining procedures, was not associated with lectin bind ...	1995	7591987
distribution of lectin binding in the testes of the musk shrew, suncus murinus.	distribution of lectin binding in the testis of the musk shrew, suncus murinus, was investigated by light and transmission electron microscopy. not only spermatogenic cells but also sertoli cells bound some lectins. canavalia ensiformis agglutinin (con a) and wheat germ agglutinin (wga, triticum vulgaris), indicating the presence of d-mannose and n-acetyl-d-glucosamine respectively, showed an intense reaction in the acrosomal region of early to late spermatids. ricinus communis agglutinin i (rca ...	1995	7591995
[histochemical study of biotinylated lectins in prostatic cancer].	we examined the staining pattern of various lectins in the malignant and benign prostatic tissues to clarify the difference of sugar residues of mucosubstances between them. the binding of different lectins (concanavalia ensiformis [con a], triticum vulgaris [wag], ricinus communis [rca-i], arachis hypogaea [pna], ulex europaeus [uea-i], glycine max [sba], and gliffonia simplicifolia ii [gsa-ii]) to cells of benign prostatic tissues (17 cases) and adenocarcinoma (54 cases) was evaluated immunohi ...	1995	7596076
effect of dietary fat on diethylnitrosamine induced hepatocarcinogenesis in wistar rats.	in a diethylnitrosamine (den) induced hepatoma model, the effect of fish oil, a rich source of eicosapentaenoic acid (epa) and docosahexaenoic acid (dha), and ground nut oil, a rich source of oleic acid (oa) and linoleic acid (la), was studied. both 20% fish oil and ground nut oil inhibited den-induced hepatocarcinogenesis in rats. contrary to general belief, an inverse correlation was observed between the development of neoplastic nodules and the amount of lipid peroxides formed in the high fat ...	1995	7656238
laboratory evaluation of chemical control of aflatoxin production in unshelled peanuts (arachis hypogaea l.).	propionic acid (ammonium salt) at 3000 mg/kg (pa1) and 5000 mg/kg (pa2) of unshelled peanuts (up); grapefruit seed extract at 5000 mg/kg (gf1) and 10,000 mg/kg (gf2); sodium orthophenylphenate at 2500 mg/kg (sop1) and 5000 mg/kg (sop2); thiabendazole 1000 mg/kg (tbz1) and 5000 mg/kg (tbz2) were studied in the laboratory, to verify their efficiency in controlling fungal growth and aflatoxin (af) production on moist up (16-18% moisture content). moist up were put into polyethylene bags with cotton ...	1995	7664926
idiotypes of pre-existing human anti-carcinoma anti-t and anti-tn antibodies.	all humans normally possess antibodies, predominantly igm, that react specifically with the thomsen-friedenreich (t) and the tn antigens which are present in immunoreactive form on > 85% of all human carcinomas, but not in healthy and otherwise diseased tissues. we report here a serological study of idiotype expression and antigen reactivity of the anti-t and anti-tn antibodies. idiotypy was analyzed with rabbit antibodies raised against, and made specific for, affinity-purified polyclonal anti- ...	1993	7680895
identification of tubular cell nephron segment origin in renal fine-needle aspirates.	currently there are no definitive criteria for identification of the nephron segment origin of tubular cells in renal fine-needle aspirates. this information would be useful for determining the cortical or medullary location of aspirate specimens. to establish morphologic criteria for identifying tubular cell origin, we evaluated 60 allograft and four native kidney aspirates with immunocytochemistry (ic) and may-grünwald-giemsa (mgg) stains. ic markers included uro-3 and tetragonolobus purpuras ...	1993	7683404
determination of the sialic acid linkage specificity of sialidases using lectins in a solid phase assay.	a procedure for the determination of activity and linkage specificity of sialidases is described. the sialoglycoprotein fetuin is coated onto a microtiter plate and incubated with sialidases from different sources. enzymatic activities and linkage specificities are then determined by a sandwich method which measured the binding of different lectins to fetuin. the lectins used were peanut agglutinin (pna) from arachis hypogaea, which binds specifically the galactose beta-1-3-n-acetylgalactosamine ...	1993	7686353
study of renal tubular glycoconjugates in tubulointerstitial damage using conjugated lectins.	changes in the glycoconjugates of renal tubules associated with tubular damage were studied by lectin histochemistry, and their possible significance was determined. the excretion of various saccharides in tubular casts may also serve as markers of renal tubular damage. renal tissues from 55 cases with various glomerular diseases including ten controls were studied. the patients were divided into two groups: one with tubulointerstitial lesions (tils) (30 cases), and the other without (15 cases). ...	1993	7688421
relationship between ricinus communis agglutinin-1 binding and nucleolar organizer regions in human gliomas.	histochemical staining using lectins from ricinus communis (rca-1), arachis hypogaea, and canavalia ensiformis was investigated in 40 human gliomas, three central neurocytomas, one human neuroblastoma cell line (imr-32), and two normal brain tissues. staining was uniform in low-grade gliomas, but heterogeneous in high-grade gliomas, particularly with rca-1. the correlation between rca-1 reactivity and cellular proliferative potential was investigated in 10 high-grade gliomas using a combined sta ...	1993	7689179
characterization of glycoconjugate expression during development of meckel's cartilage in the rat.	the staining patterns of 24 biotinylated lectins were analyzed in serial sections of the mandible of 13- to 21-day-old rat embryos by means of the avidin-biotin-peroxidase method. a ubiquitous distribution of binding sites was demonstrated after incubation with con a (canavalia ensiformis), dsl (datura stramonium; except bone matrix), and wga (triticum vulgare). ecl (erythrina cristagalli), gsl i (griffonia simplicifolia), sja (saphora japonica), vvl (vicia villosa), dba (dolichus biflorus), uea ...	1995	7717533
introgression analysis of an interspecific hybrid population in peanuts (arachis hypogaea l.) using rflp and rapd markers.	forty-six introgression lines (f10c9) from a cross between arachis hypogaea l. (2n = 4x = 40) and a. cardenasii krapov. & w. c. gregory (2n = 2x = 20) were analyzed for the introgression of a. cardenasii chromosome segments. seventy-three rflp probes and 70 rapd primers, expressing from one to four a. cardenasii-specific bands, were used to evaluate the set of introgression lines. thirty-four rflp probes and 45 rapd primers identified putative a. cardenasii introgressed chromosome segments in on ...	1995	7729680
changes in glycan distribution within the porcine interhaemal barrier during gestation.	changing patterns of glycan distribution are described in porcine placentae at 15, 19, 26, 43, 58, 69 and 109 days gestation, using a carefully selected panel of lectins that allowed partial analysis of saccharide classes and sequences. the lectins used were from galanthus nivalis, pisum sativum, phaseolus vulgaris (leukohaemagglutinin), triticum vulgaris, tetragonolobus purpureus, ulex europaeus-1, arachis hypogaea, erythrina cristagalli, glycine max, maclura pomifera, wisteria floribunda, doli ...	1995	7736552
[lectin-binding proteins of plasma membranes of a human trophoblast].	spectra of lectin-binding proteins of the human trophoblast were studied by the method affinity blotting using a set of loctins conjugated with peroxidase. the set included lectins lens culinaris (lcl), canavalia ensiformis (con a), ricinus communis (rca 1), arachis hypogaea (pna), triticum vulgaris (wga), laburnum anagyroides (lal). presence of n- and o-glycans in the composition of proteins of plasmatic membranes of a trophoblast was shown, partial characteristics of their oligosugar chains be ...	1994	7747337
histochemical study of lectin binding in the human fetal minor salivary glands.	the emerging synthesis of glycoconjugates containing specific oligosaccharides in developing human fetal labial and lingual salivary glands has been investigated by lectin histochemistry. an avidin-biotin technique was used to study the binding of lectins from ulex europeus i (uea-i), dolichos biflorus (dba), glycine maximus (sba), helix pomatia (hpa), arachis hypogaea (pna) and triticum vulgare (wga) to specific sugars on sections of tissue from labial glands, glands of blandin and nuhn, glands ...	1995	7776265
presence of vicia graminea lectin- or vicia unijuga lectin-binding (vgu) glycoproteins, vgu glycoproteins with thomsen-friedenreich (t) activity and t-active glycoproteins in human meconium.	perchloric acid-soluble fraction prepared from a mixture of meconiums of 22 newborn babies was subjected to a systematic affinity chromatography using vicia unijuga lectin-cellulofine column and arachis hypogaea lectin-agarose column and separated into four fractions, vgu glycoprotein, vgu glycoprotein with t activity, t-active glycoprotein and another glycoprotein fractions. hplc analysis showed that the first fraction contained three glycoproteins with molecular weight (mw) of about 19,400 kda ...	1995	7780836
use of lectins to evaluate the effects of gaas softlaser on dog tendon.	1. lectins labeled with colloidal gold particles were used for the ultrastructural evaluation of the biological effects of gaas softlaser irradiation on the healing of dog tendon wounds. 2. six dogs were submitted to tenotomy and tenorrhaphy on the right and left hind legs. all animals received laser irradiation (4 j/cm2) daily for ten days only on the left leg, and the right leg of the same animal was used as control. biopsies were taken 11, 22 and 40 days after surgery. 3. laser-irradiated and ...	1994	7787808
putative glycoprotein and glycolipid polymorphonuclear leukocyte receptors for the actinomyces naeslundii wvu45 fimbrial lectin.	recognition of receptors on sialidase-treated polymorphonuclear leukocytes (pmns) by the gal/galnac lectin associated with the type 2 fimbriae of certain strains of actinomyces results in activation of the pmns, phagocytosis, and destruction of the bacteria. in the present study, plant lectins were utilized as probes to identify putative pmn receptors for the actinomyces lectin. the gal-reactive lectin from ricinus communis (rcai), the gal/galnac-reactive lectins from r. communis (rcaii) and bau ...	1995	7790078
a sheep hydatid cyst glycoprotein as receptors for three toxic lectins, as well as abrus precatorius and ricinus communis agglutinins.	the binding properties of a glycoprotein with blood group p1 specificity isolated from sheep hydatid cyst fluid with gal and galnac specific lectins was investigated by quantitative precipitin and precipitin inhibition assays. the glycoprotein completely precipitated ricinus communis agglutinin (rca1), abrus precatorius agglutinin (apa) and mistletoe toxic lectin-i (ml-i). only 1.0 microgram of p1 glycoprotein was required to precipitate 50% of 5.1 micrograms ml-i nitrogen. it also reacted well ...	1995	7827100
recognition of a cd4+ mouse medullary thymocyte subpopulation by amaranthus leucocarpus lectin.	we have used the gal beta(1-->3)galnac-specific amaranthus leucocarpus lectin to isolate a thymus cell subpopulation which is different from that sorted with arachis hypogaea lectin. the cells recognized by a. leucocarpus lectin were predominantly cd4+, whereas a minor proportion of cd8+ cells (approximately 11%) were also identified. the a. leucocarpus-positive cells were located in the thymus medulla and the cortico-medullary junction. the cortex was negative for a. leucocarpus cells.	1994	7835965
lectin-histochemical analysis of glycans in ovine and bovine near-term placental binucleate cells.	chorionic binucleate cells (bnc) occur in several ruminants including cow, deer, goat and sheep. they migrate through the chorionic tight junction to fuse with uterine epithelial cells and discharge their granules into maternal connective tissue. we have compared the bnc of near-term, resin-embedded, ovine and bovine placentae using 15 biotinylated lectins and an avidinperoxidase revealing system. there was pronounced conservation of saccharides between the two species. several sub-types of n-gl ...	1994	7850870
fraction a of armadillo submandibular glycoprotein and its desialylated product as sialyl-tn and tn receptors for lectins.	fraction a of the armadillo submandibular glycoprotein (asg-a) is one of the simplest glycoproteins among mammalian salivary mucins. the carbohydrate side chains of this mucous glycoprotein have one-third of the neuac alpha 2-->6galnac (sialyl-tn) sequence and two thirds of tn (galnac alpha-->ser/thr) residues. those of the desialylated product (asg-tn) are almost exclusively unsubstituted galnac residues (tn determinant). when the binding properties of these glycoproteins were tested by a preci ...	1995	7875333
tubular atrophy in the end-stage kidney: a lectin and immunohistochemical study.	atrophic tubules in end-stage renal disease (esrd) may have various morphologic appearances: some show microscopic features of "classic" atrophic tubules (thick, wrinkled tubular basement membrane and simplified epithelium), others show "thyroidization" (round tubules with simplified epithelium and casts), and many have the appearance of "endocrine" tubules (small tubules with narrow lumina, clear cells, and relatively thin basement membranes). other tubules in esrd may be enlarged and dilated w ...	1994	7906246
immunohistochemical investigations on the pyloric glands of the ruin lizard (podarcis sicula campestris de betta).	mucous cells and enteroendocrine cells of the pyloric region of the ruin lizard (podarcis sicula campestris de betta) have been examined by lectin histochemical and immunohistochemical methods. binding to five plant lectins (canavalia ensiformis, con a; triticum vulgare, wheat germ, wgl; lotus tetragonolobus, winged pea, wpl; glycine max, soybean, sbl; arachis hypogaea, peanut, pnl) was performed to characterize glycoconjugates in the secretory products of superficial and glandular mucous cells. ...	1994	7913280
in situ characterization of the human hyalocyte.	to provide more definitive evidence regarding the cellular origin of hyalocytes and to establish a basis for their identification in tissue specimens.	1994	7945040
nutrient composition of groundnut cultures (arachis hypogaea l.) in relation to their kernel size.	seven varieties of groundnuts of varying kernel size were selected to study the relation between kernel size and nutritional quality. results indicated that no significant (r = 0.22) difference was found in oil contents among the various varieties which ranged from 46-52 percent. protein contents ranged from 17-25.2 percent. protein content of small seed varieties was high compared to bold seeded varieties, whereas sugar content of bold seeded varieties was higher. no significant differences in ...	1994	7971778
glycoconjugate expression during drosophila embryogenesis.	glycoproteins and other glycoconjugates present on the surface of many cell types have been identified and assigned various functions. the extent of variation possible in complex glycan structures and the heterogeneity of glycoconjugate expression between two apparently similar cells has been demonstrated previously by using plant lectins to survey developmental biological models. to examine the array and extent of glycoconjugate roles in embryonic drosophila neurogenesis, we have used plant lec ...	1994	8036876
differential lectin binding of onchocerca lienalis and onchocerca ochengi infective larvae following their development in simulium ornatum s.l.	lectins have been used to investigate species specific differences in carbohydrate moieties on the surface of the infective larvae of two onchocerca species following their development in simulium ornatum. of the seven fitc-labelled lectins used in this study only two, arachis hypogea (pna) and helix pomatia (hpa), bound to the surface of o. lienalis, whereas no lectin binding could be detected on the surface of o. ochengi infective larvae. this indicates that in principal lectins might be a pot ...	1994	8066375
evidence that stilbene synthases have developed from chalcone synthases several times in the course of evolution.	chalcone (chs) and stilbene (sts) synthases are related plant-specific polyketide synthases that are key enzymes in the biosynthesis of flavonoids and of stilbene phytoalexins, respectively. a phylogenetic tree constructed from 34 chs and four sts sequences revealed that the sts formed no separate cluster but grouped with chs from the same or related plants. this suggested that sts evolved from chs several times independently. we attempted to stimulate this by site-directed mutagenesis of an int ...	1994	8083886
segmental differentiation of permeability, protein glycosylation, and morphology of cultured bovine lung vascular endothelium.	the barrier function, surface biochemistry, and morphology of confluent monolayers of endothelial cells isolated from different segments of the bovine lung vasculature [microvessels (blmvec), vein (bpvec) and artery (bpaec)] were grown in culture and compared. a number of common cell surface proteins were identified along with two proteins of 46 and 48 kda found exclusively on bpvec. lectin affinity chromatography revealed multiple glycosylation differences. the lectins, arachis hypogaea (aha) a ...	1994	8123001
intracytoplasmic lumina in a case of bilaterally multifocal renal oncocytomas.	we report a case of bilaterally multifocal renal oncocytomas in a patient with chronic renal disease and a long history of hypertension. histologic study of the multiple oncocytic neoplasms with hematoxylin-eosin, periodic acid-schiff, trichrome, and colloidal iron stains revealed novel, intracytoplasmic lumina, which were accentuated by immunoperoxidase staining with high-molecular-weight cytokeratin, epithelial membrane antigen, and arachis hypogaea lectin. ultrastructural studies demonstrated ...	1994	8135631
[osteogenetic cells in myositis ossificans].	we have used the lectins arachis hypogaea (pna), canavalia ensiformis (con a) and triticum vulgaris (wga) for the examination of bone forming cells in 8 variously differentiated cases of myositis ossificans. the development of myositis ossificans could be characterized with the appearance of a wga binding cell population. in this cell population the lectin binding spots may be found in the perinuclear cytoplasm, possibly in the golgi complex. the lectin binding of the bone forming cells has disa ...	1993	8136878
characterization of a calcium-dependent protein kinase from arachis hypogea (groundnut) seeds.	a calcium-dependent protein serine/threonine kinase (gncdpk) has been detected in groundnut (arachis hypogea) seeds that specifically phosphorylates a peptide (mlcpep) representing the phosphate-accepting domain of smooth muscle myosin light chains. gncdpk has been purified to near homogeneity from the soluble fraction of groundnut seeds by ammonium sulfate precipitation, q sepharose, blue sepharose, and sephacryl 300 chromatography. the molecular weight of gncdpk is estimated to be 53,000. enzy ...	1994	8165262
production of extracellular acidic lipase by rhizopus arrhizus as a function of culture conditions.	thermophilic strain of rhizopus arrhizus accumulates an acidic lipase in culture fluid when grown in a medium containing ground nut oil, milk powder and inorganic salts. addition of 2.0% ground nut oil yielded the highest productivity of enzyme. soyabean meal and arabinose were found to be the best nitrogen and carbon sources for enzyme production respectively. addition of metal ions such as mncl2, sncl2 and cacl2 increased the enzyme productivity by 4 fold. the enzyme productivity in the fermen ...	1993	8181953
interaction of hamster submaxillary sialyl-tn and tn glycoproteins with gal, galnac and glcnac specific lectins.	hamster submaxillary glycoprotein (hsm), one of the simplest glycoproteins among mammalian salivary mucins, is composed of approximately equivalent amounts of protein, hexosamine and sialic acid. the thr and ser residues in the protein core account for more than half of all of the amino acid residues, while lys, glu, pro and ala are the major components of the remaining portion of amino acids. the carbohydrate side chains of this mucous glycoprotein have mainly the neuac-galnac-(sialyl-tn) seque ...	1994	8183285
responses to cadmium toxicity during in vitro growth in arachis hypogaea.		1994	8186648
lectins as markers of rumen epithelial cell differentiation.	lectins of different carbohydrate specificities (gna (galanthus nivalis), con a (canavalia ensiformis), vfl (vicia faba), psl (pisum sativum), lca (lens culinaris), pna (arachis hypogaea; with or without prior neuraminidase treatment), wga (triticum vulgare), sba (glycine max), uea-i (ulex europaeus), lpa (limulus polyphemus), bs-i b4 (bandeiraea simplicifolia, isolectin b4)) were explored for use as differentiation markers of rumen epithelial cells in vivo and in vitro. lectins specific for man ...	1994	8206789
immunomodulation due to coexposure to styrene and dioctyl phthalate in mice.	pathomorphological and immunological alterations caused by a mixture of styrene and dioctyl phthalate were studied in albino mice following oral administration of 0.02, 0.03, 0.05 x ld50 of the mixture. the chemicals were mixed together proportionate to their respective ld50 values and fed in ground nut oil, 5 d/wk for 4 weeks. histological examination of spleen revealed considerable depletion of cellular population of lymphoid follicles which corresponded to the dose dependent decrease in splen ...	1993	8227974
schistosoma mansoni: surface membrane isolation with lectin-coated beads.	lectins from lens culinaris and arachis hypogaea immobilized on polyacrylamide beads were used for selective isolation of glycosylated surface membrane domains of adult schistosoma mansoni worms, and the method was compared with the membrane isolation procedure developed with polycationic (affi-gel) beads. the lentil lectin proved to be suitable for interaction with surface membrane components: an increment in the specific activities of tegumental phosphohydrolases was observed in the bound frac ...	1993	8231898
immunohistochemical and lectin dissection of the human nephron in health and disease.	many renal diseases involving the tubular epithelium appear to preferentially affect certain nephron segments. while major portions of the nephron, such as proximal and distal convoluted tubules and collecting ducts, can be identified in the normal kidney, the distinction of diseased nephron segments can be difficult in tissue sections. thus, to identify which nephron segments are involved in pathologic changes is usually impossible by routine histologic examination alone. recently antibody and ...	1993	8250694
crystallographic refinement of bowman-birk type protease inhibitor a-ii from peanut (arachis hypogaea) at 2.3 a resolution.	the crystal structure of bowman-birk type protease inhibitor a-ii from peanut was refined at 2.3 a resolution using a restrained least-squares method. the crystallographic r-factor is 0.196 for 7697 reflections with f > 3 sigma (f) in the range from 6.0 to 2.3 a resolution. two molecules in an asymmetric unit are independently refined and, their structures are compared with each other. the inhibitor molecule has an elongated shape with two reactive sites, one at both ends of the longest dimensio ...	1993	8254669
intracellular lectin-binding sites in symbiont-bearing crithidia species.	crithidia oncopelti, c. deanei, and c. desouzai are flagellates of the trypanosomatidae family that present bacterium-like endosymbionts in their cytoplasm. direct and indirect lectin-gold labeling techniques were used at the electron microscopic level in lowicryl k4m-embedded cells to demonstrate the presence of intracellular lectin-binding sites. we used the lectins ulex europaeus i, griffonia simplicifolia ii, ricinus communis i, arachis hypogaea, g. simplicifolia i, wistaria floribunda, limu ...	1993	8278337
interaction of a novel tn (galnac alpha 1-->ser/thr) glycoprotein with gal, galnac and glcnac specific lectins.	a naturally occurring tn glycoprotein (native asg-tn) with galnac alpha 1-->ser/thr as the only carbohydrate side chains, has been prepared from armadillo submandibular glands. in a quantitative precipitin assay, this glycoprotein completely precipitated maclura pomifera (mpa), vicia villosa b4 (vvl-b4) and artocarpus integrifolia (jacalin, ail). it also reacted well with helix pomatia (hpl) and wistaria floribunda (wfl) and precipitated over 75% of the lectin nitrogen added, but poorly with ric ...	1994	8292029
analysis of the ability of three probes targeting the outer acrosomal membrane or acrosomal contents to detect the acrosome reaction in human spermatozoa.	a comparative analysis has been undertaken of the behaviour of probes targeting the outer acrosomal membrane (arachis hypogaea lectin) or constituents of the acrosomal vesicle (pisum sativum lectin or monoclonal antibody crb9) following the induction of the acrosome reaction in human spermatozoa with the ionophore a23187. the results obtained with these two classes of reagent were highly correlated (r = approximately 0.8), although the absolute rates of acrosome reaction were significantly diffe ...	1993	8300825