Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
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| a comparative study of flavonoid compounds, vitamin c, and antioxidant properties of baby leaf brassicaceae species. | a comparative study of antioxidant compounds, flavonoids and vitamin c, and also antioxidant activity was carried out in four species of brassicaceae vegetables used for salads: watercress ( nasturtium officinale r. br.), mizuna [ brassica rapa l. subsp. nipposinica (l.h. bailey) haneltand], wild rocket [ diplotaxis tenuifolia (l.) dc.], and salad rocket [ eruca vesicaria (l.) cav.]. the characterization of individual phenolic compounds by hplc-dad-ms/ms-esi in watercress and mizuna completes th ... | 2008 | 18321050 |
| differences in phyllotreta cruciferae and phyllotreta striolata (coleoptera: chrysomelidae) responses to neonicotinoid seed treatments. | insecticidal seed treatments are used commonly throughout the northern great plains of north america to systemically protect seedlings of canola (brassica napus l. and brassica rapa l.) from attack by the flea beetles phyllotreta cruciferae (goeze) and phyllotreta striolata (f.) (coleoptera: chrysomelidae). here, we investigated differential responses by the two flea beetle species to the neonicotinoid seed treatments thiamethoxam (helix and helix xtra) and clothianidin (prosper 400) in greenhou ... | 2008 | 18330131 |
| denaturing gradient gel electrophoresis analysis of bacterial community profiles in the rhizosphere of cry1ac-carrying brassica rapa subsp. pekinensis. | the effect of genetically modified (gm) brassica rapa subsp. pekinensis (chinese cabbage) expressing bt toxin gene (cry1ac) to the rhizosphere bacterial community was examined using the denaturing gradient gel electrophoresis (dgge) fingerprinting method. from the visual comparison of the dgge profiles, there were no significant differences between the profiles of bt and control rhizosphere in both suwon and yesan samples. from the sequence analysis of the individual bands, sphingomonas sp. of a ... | 2008 | 18337686 |
| the pattern of amplification and differentiation of ty1-copia and ty3-gypsy retrotransposons in brassicaceae species. | one of the causes of genome size expansion is considered to be amplification of retrotransposons. we determined nucleotide sequences of 24 pcr products for each of six retrotransposons in brassica rapa and brassica oleracea. phylogenetic trees of these sequences showed species-specific clades. we also sequenced stf7a homologs and tto1 homologs, 24 pcr products each, in nine diploids and three allopolyploids, and constructed phylogenetic trees. in these phylogenetic trees, species-specific clades ... | 2008 | 18379130 |
| biological testing of a digested sewage sludge and derived composts. | aiming to evaluate a possible loss of soil habitat function after amendment with organic wastes, a digested sewage sludge and derived composts produced with green residues, where biologically tested in the laboratory using soil animals (eisenia andrei and folsomia candida) and plants (brassica rapa and avena sativa). each waste was tested mimicking a field application of 6ton/ha or 12ton/ha. avoidance tests did not reveal any impact of sludge and composts to soil biota. germination and growth te ... | 2008 | 18413285 |
| toxicity of perfluorooctane sulfonate and perfluorooctanoic acid to plants and aquatic invertebrates. | acute toxicities of perfluorooctane sulfonate (pfos) and perfluorooctanoic acid (pfoa) were tested on four freshwater species and three plant species. pfos was more toxic than pfoa for all species tested in this study. similar time-response patterns of pfos and pfoa toxicity were observed for each tested species. values of the 48-h lc(50) of pfos for all test species ranged from 27 to 233 mg/l and values of the 96-h lc(50) for three of the species ranged from 10 to 178 mg/l. values of the 48-h l ... | 2009 | 18461560 |
| [isolation and identification of specific sequences correlated to cytoplasmic male sterility and fertile maintenance in cauliflower (brassica oleracea var. botrytis)]. | analysis of issr (inter-simple sequence repeat) and ddrt-pcr (differential display reverse transcriptase polymerase chain reaction) was performed between cytoplasmic male sterility cauliflower ogura-a and its corresponding maintainer line ogura-b. totally, 306 detectable bands were obtained by issr using thirty oligonucleotide primers. commonly, six to twelve bands were produced per primer. among all these primers only the amplification of primer issr3 was polymorphic, an 1100 bp specific band w ... | 2008 | 18464586 |
| conserved patterns of chromosome pairing and recombination in brassica napus crosses. | the patterns of chromosome pairing and recombination in two contrasting brassica napus f1 hybrids were deduced. one hybrid was from a winter oilseed rape (wosr) x spring oilseed rape cross, the other from a resynthesized b. napus x wosr cross. segregation at 211 equivalent loci assayed in the population derived from each hybrid produced two collinear genetic maps. alignment of the maps indicated that b. napus chromosomes behaved reproducibly as 19 homologous pairs and that the 19 distinct chromo ... | 1997 | 18464842 |
| chromosomal localization and characterization of rdna loci in the brassica a and c genomes. | using fluorescence in situ hybridization, we located ribosomal dna loci on prometaphase chromosomes of the diploid species brassica rapa and brassica oleracea and their amphidiploid brassica napus. based on comparisons of chromosome morphology and hybridization patterns, we characterized the individual b. napus rdna loci according to their presumed origins in the brassica a and c genomes. as reported in other studies, the sum of rdna loci observed on b. rapa (aa genome) and b. oleracea (cc genom ... | 1997 | 18464849 |
| inheritance and expression patterns of bn28, a low temperature induced gene in brassica napus, throughout the brassicaceae. | molecular genetics is becoming an important tool in the breeding and selection of agronomically important traits. bn28 is a low temperature induced gene in brassicaceae species. pcr and southern blot analysis indicate that bn28 is polymorphic in the three diploid genomes: brassica rapa (aa), brassica nigra (bb), and brassica oleracea (cc). of the allotetraploids, brassica napus (aacc) is the only species to have inherited homologous genes from both parental genomes. brassica juncea (aabb) and br ... | 1996 | 18469930 |
| metal ion-inducing metabolite accumulation in brassica rapa. | plants face a number of biotic and abiotic environmental stress factors during growth. among the abiotic factors, in particular, a great deal of attention has been paid to metals not only because of their increasing amounts in the environment due to rapid industrial development but also because of the variation of metal composition in soil. cultivation of crops close to industrial areas or irrigation with contaminated water may result in both growth inhibition and tissue accumulation of metals. ... | 2008 | 18541336 |
| an intensive understanding of vacuum infiltration transformation of pakchoi (brassica rapa ssp. chinensis). | pakchoi (brassica rapa l. ssp. chinensis), a kind of chinese cabbage, is an important vegetable in asian countries. agrobacterium mediated in planta vacuum infiltration transformation has been performed in pakchoi since 1998, but a detailed study on this technique was lacking. pakchoi plants 40-50 days old with inflorescences were vacuum infiltrated with agrobacterium tumefaciens strain c58c1 harboring the binary vector pbbbast-gus-intron. the transformation frequency in the harvested seeds main ... | 2008 | 18542966 |
| the first generation of a bac-based physical map of brassica rapa. | the genus brassica includes the most extensively cultivated vegetable crops worldwide. investigation of the brassica genome presents excellent challenges to study plant genome evolution and divergence of gene function associated with polyploidy and genome hybridization. a physical map of the b. rapa genome is a fundamental tool for analysis of brassica "a" genome structure. integration of a physical map with an existing genetic map by linking genetic markers and bac clones in the sequencing pipe ... | 2008 | 18549474 |
| a change in climate causes rapid evolution of multiple life-history traits and their interactions in an annual plant. | climate change is likely to spur rapid evolution, potentially altering integrated suites of life-history traits. we examined evolutionary change in multiple life-history traits of the annual plant brassica rapa collected before and after a recent 5-year drought in southern california. we used a direct approach to examining evolutionary change by comparing ancestors and descendants. collections were made from two populations varying in average soil moisture levels, and lines propagated from the c ... | 2008 | 18557796 |
| characterization of conserved and novel micrornas and their targets, including a tumv-induced tir-nbs-lrr class r gene-derived novel mirna in brassica. | nine conserved mirna families and three potential novel mirnas in brassica rapa were identified from a small rna library. the expression patterns of some conserved mirnas had different tissue specificity in brassica and arabidopsis. one of the three potential mirnas, named bra-mir1885, was verified as a true functional mirna. it could be induced specifically by turnip mosaic virus (tumv) infection, and target tir-nbs-lrr class disease-resistant transcripts for cleavage. based on the hypothesis f ... | 2008 | 18558089 |
| quantitative trait loci for glucosinolate accumulation in brassica rapa leaves. | glucosinolates and their breakdown products have been recognized for their effects on plant defense, human health, flavor and taste of cruciferous vegetables. despite this importance, little is known about the regulation of the biosynthesis and degradation in brassica rapa. here, the identification of quantitative trait loci (qtl) for glucosinolate accumulation in b. rapa leaves in two novel segregating double haploid (dh) populations is reported: dh38, derived from a cross between yellow sarson ... | 2008 | 18564302 |
| scenario modeling potential eco-efficiency gains from a transition to organic agriculture: life cycle perspectives on canadian canola, corn, soy, and wheat production. | we used life cycle assessment to scenario model the potential reductions in cumulative energy demand (both fossil and renewable) and global warming, acidifying, and ozone-depleting emissions associated with a hypothetical national transition from conventional to organic production of four major field crops [canola (brassica rapa), corn (zea mays), soy (glycine max), and wheat (triticum aestivum)] in canada. models of these systems were constructed using a combination of census data, published va ... | 2008 | 18574623 |
| molecular cloning and characterization of the dicer-like 2 gene from brassica rapa. | dicer-like proteins (dcls) are involved in small rna-mediated development and viral defense in plants. in model plants, at least four dcls have been found and a number of studies have helped to understand their function. however, the function of the dicer or dcls in other plants is still unclear. here, we report the full-length cdna sequence of brassica rapa ssp. chinensis dcl2 (brdcl2) gene, which contains a 4,179 bp open reading frame (orf) encoding a protein of 1,392 amino acids. at the 3' en ... | 2009 | 18607769 |
| mapping of isolate-specific qtls for clubroot resistance in chinese cabbage (brassica rapa l. ssp. pekinensis). | a number of clubroot resistant (cr) chinese cabbage cultivars have been developed in japan using resistant genes from cr european fodder turnips (b. rapa ssp. rapifera). clubroot resistance in european fodder turnips are known to be controlled by the combined action of several dominant resistance genes. we have developed three chinese cabbage clubroot-resistant doubled haploid (dh) lines--t136-8, k10, and c9--which express resistance in different manners against two isolates of plasmodiophora br ... | 2008 | 18612625 |
| the korea brassica genome project: a glimpse of the brassica genome based on comparative genome analysis with arabidopsis. | a complete genome sequence provides unlimited information in the sequenced organism as well as in related taxa. according to the guidance of the multinational brassica genome project (mbgp), the korea brassica genome project (kbgp) is sequencing chromosome 1 (cytogenetically oriented chromosome #1) of brassica rapa. we have selected 48 seed bacs on chromosome 1 using est genetic markers and fish analyses. among them, 30 bac clones have been sequenced and 18 are on the way. comparative genome ana ... | 2005 | 18629219 |
| computational tools for brassica-arabidopsis comparative genomics. | recent advances, such as the availability of extensive genome survey sequence (gss) data and draft physical maps, are radically transforming the means by which we can dissect brassica genome structure and systematically relate it to the arabidopsis model. hitherto, our view of the co-linearities between these closely related genomes had been largely inferred from comparative rflp data, necessitating substantial interpolation and expert interpretation. sequencing of the brassica rapa genome by th ... | 2005 | 18629221 |
| transcription of the cauliflower mosaic virus genome in isolated nuclei from turnip leaves. | nuclei isolated from turnip (brassica rapa l. c.v. just right) leaves infected with cauliflower mosaic virus synthesize rna in vitro which hybridizes to purified cauliflower mosaic virus dna. nuclei isolated from uninfected leaves do not produce these viral transcripts in vitro. viral-specific transcription in isolated nuclei is catalyzed by endogenous dna-dependent rna polymerase 11 based on sensitivity to alpha-amanitin and ionic strength optima. only one strand of the viral genome is transcri ... | 1980 | 18631807 |
| plant phenolics affect oxidation of tryptophan. | the effect of berry phenolics such as anthocyanins, ellagitannins, and proanthocyanidins from raspberry (rubus idaeus), black currant (ribes nigrum), and cranberry (vaccinium oxycoccus) and byproducts of deoiling processes rich in phenolics such as rapeseed (brassica rapa l.), camelina (camelina sativa), and soy (glycine max l.) as well as scots pine bark (pinus sylvestris) was investigated in an h2o2-oxidized tryptophan (trp) solution. the oxidation of trp was analyzed with high-performance liq ... | 2008 | 18646765 |
| phytotoxic effects of sewage sludge extracts on the germination of three plant species. | in order to evaluate the ability of three types of extracts to explain the ecotoxicological risk of treated municipal sewage sludges, the oecd 208a germination test was applied using three plants (lolium perenne l., brassica rapa l., and trifolium pratense l.). three equivalent batches of sludge, remained as dewatered sludge, composted with plant remains and thermally dried, from an anaerobic waste water treatment plant were separated. samples from these three batches were extracted in water, me ... | 2008 | 18651217 |
| effect of crop improvement on genetic diversity in oilseed brassica rapa (turnip-rape) cultivars, detected by ssr markers. | with the improvement of seed quality, brassica rapa oilseed germplasm went through 2 major breeding bottlenecks during the introgression of genes for zero erucic acid content and low glucosinolate content, respectively. this study investigates the impact of these bottlenecks on the genetic diversity in european winter b. rapa by comparing 3 open-pollinated cultivars, each representing a different breeding period. diversity was estimated on 32 plants per cultivar, with 16 simple sequence repeat ( ... | 2008 | 18670055 |
| growth inhibition in chinese cabbage (brassica rapa var. chinensis) growth exposed to di-n-butyl phthalate. | the toxicity and effects of di-n-butyl phthalate (dbp), an endocrine disruptor, on the growth of chinese cabbage (brassica rapa var. chinensis) were studied. etiolation occurred on leaves of chinese cabbage plant treated with 50mg/l of dbp for 42 d. dbp even below 1mg/l had a significant effect on the concentration of chlorophyll in chinese cabbage and the biomass showed a severe decrease under treatment with more than 30 mg/l of dbp. at a concentration below 1mg/l of dbp, no significant differe ... | 2009 | 18678443 |
| comparative innate responses of the aphid parasitoid diaeretiella rapae to alkenyl glucosinolate derived isothiocyanates, nitriles, and epithionitriles. | cruciferous plants (brassicaceae) are characterized by the accumulation of a group of secondary metabolites known as glucosinolates that, following attack by pathogens or herbivores, may be hydrolyzed to one of a number of products including isothiocyanates and nitriles. despite the range of hydrolysis products that may be produced, the toxicity of glucosinolates to pathogens and herbivores may be explained largely by the production of isothiocyanates. isothiocyanates are also known to provide a ... | 2008 | 18712443 |
| alternative transcription initiation of the nitrilase gene (brnit2) caused by infection with plasmodiophora brassicae woron. in chinese cabbage (brassica rapa l.). | in clubroot disease, gall formation is induced by infection with the obligate biotroph plasmodiophora brassicae, and cell hypertrophy is dependent on increased auxin levels. the enzyme nitrilase is suggested to play an important role in auxin biosynthesis in plants. here, we investigated the expression of nitrilase genes in clubroot disease in chinese cabbage (brassica rapa l.). we isolated four isogenes of nitrilase (brnit1, brnit2, brnit3, and brnit4) from chinese cabbage. when a brnit2-specif ... | 2008 | 18726557 |
| the cacta transposon bot1 played a major role in brassica genome divergence and gene proliferation. | we isolated and characterized a brassica c genome-specific cacta element, which was designated bot1 (brassica oleracea transposon 1). after analysing phylogenetic relationships, copy numbers and sequence similarity of bot1 and bot1 analogues in b. oleracea (c genome) versus brassica rapa (a genome), we concluded that bot1 has encountered several rounds of amplification in the oleracea genome only, and has played a major role in the recent rapa and oleracea genome divergence. we performed in sili ... | 2008 | 18764926 |
| transcriptome analysis in brassica rapa under the abiotic stresses using brassica 24k oligo microarray. | genome wide transcription analysis in response to stresses is essential to provide the basis of effective engineering strategies to improve stress tolerance in crop plants. in order to perform transcriptome analysis in brassica rapa, we constructed a b. rapa oligo microarray, kbgp-24k, using sequence information from approximately 24,000 unigenes and analyzed cold (4 degrees c), salt (250 mm nacl), and drought (air-dry) treated b. rapa plants. among the b. rapa unigenes represented on the microa ... | 2008 | 18797175 |
| broadening the genetic basis of verticillium longisporum resistance in brassica napus by interspecific hybridization. | abstract verticillium wilt caused by the vascular fungal pathogen verticillium longisporum is one of the most important pathogens of oilseed rape (brassica napus sp. oleifera) in northern europe. because production of this major oilseed crop is expanding rapidly and no approved fungicides are available for v. longisporum, long-term control of the disease can only be achieved with cultivars carrying effective quantitative resistance. however, very little resistance to v. longisporum is available ... | 2007 | 18943507 |
| induction of soil suppressiveness against rhizoctonia solani by incorporation of dried plant residues into soil. | abstract suppressive effects of soil amendment with residues of 12 cultivars of brassica rapa on damping-off of sugar beet were evaluated in soils infested with rhizoctonia solani. residues of clover and peanut were tested as noncruciferous controls. the incidence of damping-off was significantly and consistently suppressed in the soils amended with residues of clover, peanut, and b. rapa subsp. rapifera 'saori', but only the volatile substance produced from water-imbibed residue of cv. saori ex ... | 2006 | 18943670 |
| occurrence of a new subclade of leptosphaeria biglobosa in western australia. | stem canker of crucifers is caused by an ascomycete species complex comprising of two main species, leptosphaeria maculans and l. biglobosa. these are composed of at least seven distinct subclades based on biochemical data or on sequences of internal transcribed spacer (its), the mating type mat1-2 or fragments of actin or beta-tubulin genes. in the course of a wide-scale characterization of the race structure of l. maculans from western australia, a few isolates from two locations failed to amp ... | 2008 | 18944083 |
| biological and phylogenetic characterization of fusarium oxysporum complex, which causes yellows on brassica spp., and proposal of f. oxysporum f. sp. rapae, a novel forma specialis pathogenic on b. rapa in japan. | although the causal agent of yellows of brassica rapa (turnip, pak choi, and narinosa) in japan was reported in 1996 to be fusarium oxysporum f. sp. conglutinans, this classification has remained inconclusive because of a lack of detailed genetic and pathogenic studies. therefore, we analyzed the taxonomic position of this organism using japanese isolates of f. oxysporum complex obtained from diseased individuals of various b. rapa subspecies. phylogenetic analyses using partial sequences of the ... | 2008 | 18944198 |
| identification of novel splice variants of the arabidopsis dcl2 gene. | in arabidopsis thaliana, dicer-like protein 2 (dcl2) cleaves double-stranded virus rna, playing an essential role in the rna interference pathway. here, we describe three alternative splicing (as) forms of atdcl2: in one, both intron 8 and intron 10 are retained in the mrna, in second only intron 8 is retained and in the third no intron is retained, but there is a deletion of 56 nucleotides at the end of exon 10. these splicing forms are present in stems and leaves at different development stage ... | 2009 | 19011859 |
| map-based cloning and characterization of a gene controlling hairiness and seed coat color traits in brassica rapa. | a glabrous, yellow-seeded doubled haploid (dh) line and a hairy, black-seeded dh line in chinese cabbage (b. rapa) were used as parents to develop a dh line population that segregated for both hairiness and seed coat color traits. the data showed that both traits completely co-segregated each other, suggesting that one mendelian locus controlled both hairiness and seed coat color in this population. a fine genetic map was constructed and a snp marker that was located inside a brassica ortholog o ... | 2009 | 19039665 |
| diversity, distribution and role of wild crucifers in major cabbage and kale growing areas of kenya. | an investigation of the diversity and distribution of wild crucifer species and their importance for cultivated crucifers was conducted during 2005 and 2006 in the highland and mid-altitude semi-arid areas of kenya. thirteen species of wild crucifers in nine genera were recorded: raphanus raphanistrum, erucastrum arabicum, sisymbrium officinale, crambe kilimandscharica, capsella bursa-pastoris, rorippa nudiuscula, ro. micrantha, ro. microphylla, lepidium bonariense, coronopus didymus, brassica r ... | 2009 | 19063754 |
| targeted metabolite analysis and biological activity of pieris brassicae fed with brassica rapa var. rapa. | for the first time, an insect-plant system, pieris brassicae fed with brassica rapa var. rapa, was tested for its biological capacity, namely, antioxidant (dpph*, *no, and o(2)*- radicals) and antimicrobial (bacteria and fungi) activities. samples from the insect's life cycle (larvae, excrements, exuviae, and butterfly) were always found to be more efficient than the host plant. also, p. brassicae materials, as well as its host plant, were screened for phenolics and organic acids. the host plant ... | 2009 | 19115952 |
| impact of decreasing ratios of insecticide-treated seed on flea beetle (coleoptera: chrysomelidae, phyllotreta spp.) feeding levels and canola seed yields. | field studies were conducted at two locations on the canadian prairies to investigate use of reduced ratios of insecticide-treated seed in controlling flea beetle (coleoptera: chrysomelidae, phyllotreta spp.) damage to canola (brassica napus l. and brassica rapa l.). five treatments were evaluated: bare seed control, fungicide-only (0x), and three ratios of insecticide plus fungicide in proportions of all (1x), two thirds (0.67x), or one third (0.33x) of the seeds coated with insecticide. decrea ... | 2008 | 19133461 |
| emergence and seasonal activity of the entomophagous rove beetle aleochara bilineata (coleoptera: staphylinidae) in canola in western canada. | aleochara bilineata gyllenhal (coleoptera: staphylinidae) is an important natural enemy of root maggots (delia spp.) (diptera: anthomyiidae), which are serious pests of brassicaceous crops in north america and europe. adults of a. bilineata feed on eggs and larvae of root maggots, and a. bilineata larvae parasitize delia spp. pupae. emergence and seasonal activity patterns of a. bilineata were investigated during 2003-2005 in canola (brassica rapa l. and brassica napus l.) in central alberta, ca ... | 2008 | 19161688 |
| determination of n+1 gamete transmission rate of trisomics and location of gene controlling 2n gamete formation in chinese cabbage (brassica rapa). | a set of trisomics of chinese cabbage was used for determining the n+1 gamete transmission rate and locating the gene controlling 2n gamete formation on the corresponding chromosome. the results showed that the transmission rates of extra chromosomes in different trisomics varied from 0% to 15.38% by male gametes and from 0% to 17.39% by female gametes. of the nine f(2) populations derived from the hybridizations between each trisomic and bp058 (2n gamete material), only tri-4xbp058 showed that ... | 2009 | 19166491 |
| a naturally occurring splicing site mutation in the brassica rapa flc1 gene is associated with variation in flowering time. | flowering locus c (flc), encoding a mads-domain transcription factor in arabidopsis, is a repressor of flowering involved in the vernalization pathway. this provides a good reference for brassica species. genomes of brassica species contain several flc homologues and several of these colocalize with flowering-time qtl. here the analysis of sequence variation of brflc1 in brassica rapa and its association with the flowering-time phenotype is reported. the analysis revealed that a g-->a polymorphi ... | 2009 | 19190098 |
| spontaneous capture of oilseed rape (brassica napus) chloroplasts by wild b. rapa: implications for the use of chloroplast transformation for biocontainment. | environmental concerns over the cultivation of genetically modified (gm) crops largely centre on the ecological consequences following gene flow to wild relatives. one attractive solution is to deploy biocontainment measures that prevent hybridization. chloroplast transformation is the most advanced biocontainment method but is compromised by chloroplast capture (hybridization through the maternal lineage). to date, however, there is a paucity of information on the frequency of chloroplast captu ... | 2009 | 19198841 |
| effects of vegetable oil residue after soil extraction on physical-chemical properties of sandy soil and plant growth. | vegetable oil has the ability to extract polycyclic aromatic hydrocarbons (pahs) from contaminated sandy soil for a remediation purpose, with some of the oil remaining in the soil. although most of the pahs were removed, the risk of residue oil in the soil was not known. the objective of this study was to evaluate the effects of the vegetable oil residue on higher plant growth and sandy soil properties after soil extraction for a better understanding of the soil remediation. addition of sunflowe ... | 2008 | 19209632 |
| impact of cry1ac-carrying brassica rapa subsp. pekinensis on leaf bacterial community. | the effects of chinese cabbage (brassica rapa subsp. pekinensis) carrying cry1ac derived from bacillus thuringiensis (bt) on leaf bacterial community were examined by analyzing the horizontal transfer of trans-gene fragments from plants to bacteria. the effect of plant pathogenic bacteria on the gene transfer was also examined using pseudomonas syringae pathovar. maculicola. the frequency of hygromycin-resistant bacteria did not alter in bt leaves, though slight increase was observed in pseudomo ... | 2009 | 19229489 |
| plantgm: a database for genetic markers in rice (oryza sativa) and chinese cabbage (brassica rapa). | the plant genetic map database (plantgm) has been developed as a web-based system which provides information about genetic markers in rice (oryza sativa) and chinese cabbage (brassica rapa). the database has three major parts and functions; (1) map search, (2) marker search, and (3) qtl search. at present, the database provides characterization information for about 3258 genetic markers. it has 2800 rflp and 112 qtl markers related to rice in addition to 321 rflp and 25 pcr-based markers for chi ... | 2008 | 19238232 |
| chromosome elimination, addition and introgression in intertribal partial hybrids between brassica rapa and isatis indigotica. | partial hybrids with female-parent-type phenotypes and chromosome numbers but altered genomic compositions have been reported in wide crosses of several plants. in order to introgress desirable genes from a wild relative, isatis indigotica (a dye and medicinal plant; 2n = 14), into brassica crops, intertribal sexual hybridizations were carried out with b. rapa (2n = 20), and the resulting hybrids and their progenies were characterized. | 2009 | 19258339 |
| sewage sludge applied to agricultural soil: ecotoxicological effects on representative soil organisms. | application of sewage sludge to agricultural lands is a current practice in eu. european legislation permits its use when concentrations of metals in soil do not increase above the maximum permissible limits. in order to assess the fate and the effects on representative soil organisms of sewage sludge amendments on agricultural lands, a soil microcosm (multi-species soil system-ms3) experiment was performed. the ms3 columns were filled with spiked soil at three different doses: 30, 60 and 120tha ... | 2009 | 19261330 |
| host suitability of rapeseed for heterodera schachtii. | because rapeseed, especially canola, has the potential to be grown in rotation with sugarbeet in the north-central region of the united states, this study was initiated to assess its susceptibility to infection by heterodera schachtii and to develop a screening method for brassica germplasm. existing methodology was adapted for growing brassica juncea, b. napus, b. rapa, brassica hybrids, and sugarbeet, beta vulgaris, in h. schachtii-infested soil to count the females that developed on the roots ... | 2003 | 19265972 |
| responses of some common cruciferae to root-knot nematodes. | ten cultivated plants of the family cruciferae were evaluated for susceptibility to meloidogyne arenaria race 1, m. incognita races 1 and 3, and m. javanica in a series of four separate greenhouse tests. after 62-64 days, or 1,032-1,072 degree days (10 c base), several of the crops evaluated showed moderate to severe levels of galling (> 3.0 on 0-5 scale) and moderate numbers of egg masses (>2.0 on 0-5 scale) in response to each of the nematode species and races. among the plants tested, collard ... | 1995 | 19277321 |
| in-vitro and in-vivo effects of aldicarb on survival and development of heterodera schachtii. | aqueous solutions of 5-500 mug/ml aldicarb inhibited hatching of heterodera schachtii. addition of hatching agents, zinc chloride, or sugarbeet root diffusate, to the aldicarb solutions did not decrease the inhibition of hatching. when cysts were removed from the aldicarb solufions and then treated for 4 wk in sugarbeet root diffusate, larvae hatched and emerged. treatments of newly hatched larvae of h. schachtii with 5-100 mug/ml aldicarb depressed later development of larvae on sugarbeet (beta ... | 1975 | 19308173 |
| 5 alpha-reductase and aromatase inhibitory constituents from brassica rapa l. pollen. | in the screening of biologically active constituents from brassica rapa pollen, the supercritical co(2) fluid extract (sfe-co(2)) showed potent 5 alpha-reductase and aromatase inhibiting activity. the sfe-co(2) extract was separated by various chromatographic methods to give two new phytosterol derivatives, 24-methylenecholesterol linolenate (1) and cycloeucalenol linolenate (2), as well as eight known compounds, 24-methylenecholesterol palmitate (3), cycloeucalenol (4), pollinastanol (5), 24-me ... | 2009 | 19336936 |
| differential expression of duplicated peroxidase genes in the allotetraploid brassica napus. | gene redundancy due to polyploidization provides a selective advantage for plant adaptation. we examined the expression patterns of two peroxidase genes (bnpox1 and bnpox2) in the natural allotetraploid brassica napus and the model diploid progenitors brassica rapa (br) and brassica oleracea (bo) in response to the fungal pathogen sclerotinia sclerotiorum. we demonstrated that the bo homeolog of bnpox1 was up-regulated after infection, while both bnpox2 homeologs were down-regulated. a bias towa ... | 2009 | 19345111 |
| metabolic changes in agrobacterium tumefaciens-infected brassica rapa. | agrobacterium has the ability to transfer its genetic material, t-dna, into the plant genome. the unique interaction between the bacterium and its host plant has been well studied at the transcriptome, but not at the metabolic level. for a better understanding of this interaction it is necessary to investigate the metabolic changes of the host plant upon infection with agrobacterium tumefaciens. this study investigated the metabolic response of brassica rapa to infection with disarmed and tumor- ... | 2009 | 19346030 |
| formation of green-blue compounds in brassica rapa root by high pressure processing and subsequent storage. | the effect of high pressure treatment on biochemical changes during storage was investigated using brassica rapa root. high pressure treated samples with 400 and 600 mpa formed unique green-blue color during 7-d storage at 4 degrees c. the mechanism of green-blue compound formation would be based on biochemical pathway for a unique green-blue pigment synthesis, containing o2-dependent steps and possibly enzymatic reactions. | 2009 | 19352022 |
| gene transferability from transgenic brassica napus l. to various subspecies and varieties of brassica rapa. | gene transferability from transgenic rapeseed to various subspecies and varieties of brassica rapa was assessed in this study. artificial crossability was studied in 118 cultivars of 7 b. rapa subspecies and varieties with the transgenic rapeseed gt73 (brassica napus) as the pollen donor. on average 5.7 seeds were obtained per pollination, with a range from 0.05 to 19.4. the heading type of b. rapa l. showed significantly higher crossability than non-heading types of b. rapa. the spontaneous out ... | 2009 | 19357986 |
| physical localization and genetic mapping of the fertility restoration gene rfo in canola (brassica napus l.). | the ogu cytoplasm for male sterility and its fertility restorer gene rfo in canola (brassica napus l.) were originally introgressed from radish (raphanus sativus l.) and have been widely used for canola hybrid production and breeding. the objective of this study was to determine the physical location of the rfo locus in the canola genome using fluorescence in situ hybridization and genetic mapping. for physical localization of the rfo gene, two bacterial artificial chromosome (bac) clones, g62 a ... | 2009 | 19370095 |
| plant fitness assessment for wild relatives of insect resistant crops. | risk assessments of new insect-resistant crops will need to estimate the potential for increased weediness of wild crop relatives as a consequence of gene flow. when field experiments are precluded by containment concerns, simulation experiments can identify hazards or measure expected differences between gmos and parental plants. to measure plant fitness consequences of wild plant protection from bt-susceptible herbivores, we used topical sprays of bacterial bacillus thuringiensis larvacide (bt ... | 2009 | 19419653 |
| the cost of defense against herbivores: an experimental study of trichome production in brassica rapa. | 1993 | 19426086 | |
| a newly-developed community microarray resource for transcriptome profiling in brassica species enables the confirmation of brassica-specific expressed sequences. | the brassica species include an important group of crops and provide opportunities for studying the evolutionary consequences of polyploidy. they are related to arabidopsis thaliana, for which the first complete plant genome sequence was obtained and their genomes show extensive, although imperfect, conserved synteny with that of a. thaliana. a large number of est sequences, derived from a range of different brassica species, are available in the public database, but no public microarray resourc ... | 2009 | 19426481 |
| ssr and scar mapping of a multiple-allele male-sterile gene in chinese cabbage (brassica rapa l.). | the genic multiple-allele inherited male-sterile gene ms in chinese cabbage (brassica rapa l.) was identified as a spontaneous mutation. applying this gene to hybrid seed production, several b. rapa cultivars have been successfully bred in china. a bc(1) population (244 plants) was constructed for mapping the ms gene. screening 268 simple sequence repeat (ssr) markers which cover the entire genome of chinese cabbage was performed with bulked segregant analysis (bsa). on the basis of linkage anal ... | 2009 | 19436990 |
| isolation and characterization of ngrlk1, a receptor-like kinase of nicotiana glutinosa that interacts with the elicitin of phytophthora capsici. | elicitins, extracellular proteins from phytophthora fungi, elicit a hypersensitivity response (hr), including systemic acquired resistance, in some plants. the elicitin capsicein (approximately 10 kda) was purified by fplc from culture filtrates of p. capsici. purified native and recombinant capsicein induced a hypersensitive response in leaves of the non-host plants nicotiana glutinosa and brassica rapa subsp. pekinensis. to search for candidate capsicein-interacting proteins from n. glutinosa, ... | 2010 | 19449126 |
| genome-wide identification of glucosinolate synthesis genes in brassica rapa. | glucosinolates play important roles in plant defense against herbivores and microbes, as well as in human nutrition. some glucosinolate-derived isothiocyanate and nitrile compounds have been clinically proven for their anticarcinogenic activity. to better understand glucosinolate biosynthesis in brassica rapa, we conducted a comparative genomics study with arabidopsis thaliana and identified total 56 putative biosynthetic and regulator genes. this established a high colinearity in the glucosinol ... | 2009 | 19456863 |
| interaction between plants and bacteria: glucosinolates and phyllospheric colonization of cruciferous vegetables by enterobacter radicincitans dsm 16656. | for determining interactive plant-bacterial effects between glucosinolates and phyllospheric colonization by a plant growth-promoting strain, enterobacter radicincitans dsm 16656, in cruciferous vegetables, the extent of bacterial colonization was assessed in 5 cruciferous vegetables (brassica juncea, brassica campestris, brassica oleracea var. capitata, brassica rapa var. alboglabra, nasturtium officinale) using a species-specific taqman probe and quantitative real-time pcr. colonization abilit ... | 2009 | 19556746 |
| [cdna-aflp analysis on transcripts associated with bolting in brassica rapa l. ssp. pekinensis]. | premature bolting, caused by low temperature in spring and summer cultivation in low land and high land respectively, leads to reduction of the yield and quality of the harvested products in chinese cabbage. therefore, exploring genes involved in vernalization response is important to the improvement of chinese cabbage varieties. here, one extremely early bolting line (dh-54) and one extremely late bolting line (dh-43) were employed, and the cdna-aflp approach was used to identify key components ... | 2009 | 19586882 |
| auxin amidohydrolases from brassica rapa cleave the alanine conjugate of indolepropionic acid as a preferable substrate: a biochemical and modeling approach. | two auxin amidohydrolases, briar3 and brill2, from chinese cabbage [brassica rapa l. ssp. pekinensis (lour.) hanelt] were produced by heterologous expression in escherichia coli, purified, and screened for activity towards n-(indol-3-ylacetyl)-l-alanine (iaa-ala) and the long-chain auxin-amino acid conjugates, n-[3-(indol-3-yl)propionyl]-l-alanine (ipa-ala) and n-[4-(indol-3-yl)butyryl]-l-alanine (iba-ala). ipa-ala was shown to be the favored substrate of both enzymes, but brill2 was approximate ... | 2009 | 19602499 |
| comparative analysis between homoeologous genome segments of brassica napus and its progenitor species reveals extensive sequence-level divergence. | homoeologous regions of brassica genomes were analyzed at the sequence level. these represent segments of the brassica a genome as found in brassica rapa and brassica napus and the corresponding segments of the brassica c genome as found in brassica oleracea and b. napus. analysis of synonymous base substitution rates within modeled genes revealed a relatively broad range of times (0.12 to 1.37 million years ago) since the divergence of orthologous genome segments as represented in b. napus and ... | 2009 | 19602626 |
| genotypes of brassica rapa respond differently to plant-induced variation in air co2 concentration in growth chambers with standard and enhanced venting. | growth chambers allow measurement of phenotypic differences among genotypes under controlled environment conditions. however, unintended variation in growth chamber air co2 concentration ([co2]) may affect the expression of diverse phenotypic traits, and genotypes may differ in their response to variation in [co2]. we monitored [co2] and quantified phenotypic responses of 22 brassica rapa genotypes in growth chambers with either standard or enhanced venting. [co2] in chambers with standard venti ... | 2009 | 19603146 |
| transient expression of a foreign gene by direct incorporation of dna into intact plant tissue through vacuum infiltration. | we previously established a method to induce transient expression of foreign genes in intact plant tissue to detect the subcellular localization of proteins. here, we have inserted a putative bzip protein hy5 gene (seqid: eu386772), isolated from the seedlings of turnips brassica rapa l. subsp. rapa 'tsuda,' and a receptor-like kinase gene atrlk (seqid: ay531551.1), isolated from arabidopsis, into the plasmid pa7-gfp. we accomplished the direct incorporation of dna into onion epidermal tissue by ... | 2009 | 19618274 |
| jasmonate-dependent plant defense restricts thrips performance and preference. | the western flower thrips (frankliniella occidentalis [pergande]) is one of the most important insect herbivores of cultivated plants. however, no pesticide provides complete control of this species, and insecticide resistance has emerged around the world. we previously reported the important role of jasmonate (ja) in the plant's immediate response to thrips feeding by using an arabidopsis leaf disc system. in this study, as the first step toward practical use of ja in thrips control, we analyze ... | 2009 | 19635132 |
| hypergravity prevents seed production in arabidopsis by disrupting pollen tube growth. | how tightly land plants are adapted to the gravitational force (g) prevailing on earth has been of interest because unlike many other environmental factors, g presents as a constant force. ontogeny of mature angiosperms begins with an embryo that is formed after tip growth by a pollen tube delivers the sperm nucleus to the egg. because of the importance to plant fitness, we have investigated how gravity affects these early stages of reproductive development. arabidopsis thaliana (l.) heynh. plan ... | 2009 | 19649651 |
| enhancement of tolerance to soft rot disease in the transgenic chinese cabbage (brassica rapa l. ssp. pekinensis) inbred line, kenshin. | we developed a transgenic chinese cabbage (brassica rapa l. ssp. pekinensis) inbred line, kenshin, with high tolerance to soft rot disease. tolerance was conferred by expression of n-acyl-homoserine lactonase (ahl-lactonase) in chinese cabbage through an efficient agrobacterium-mediated transformation method. to synthesize and express the ahl-lactonase in chinese cabbage, the plant was transformed with the aii gene (ahl-lactonase gene from bacillus sp. gh02) fused to the pinii signal peptide (pr ... | 2009 | 19680657 |
| simultaneous identification of glucosinolates and phenolic compounds in a representative collection of vegetable brassica rapa. | brassica raparapa group is widely distributed and consumed in northwestern spain. the consumption of brassica vegetables has been related to human health due to their phytochemicals, such as glucosinolates and phenolic compounds that induce a variety of physiological functions including antioxidant activity, enzymes regulation and apoptosis control and the cell cycle. for first time in brassica crops, intact glucosinolates and phenolic compounds were simultaneously identified and characterized. ... | 2009 | 19683241 |
| genetic and physiological determinants of streptomyces scabies pathogenicity. | summary common scab is a severe disease worldwide affecting tap root crops and potato tubers. it is caused by soil-borne filamentous bacteria belonging to the genus streptomyces. streptomycetes usually are saprophytic microorganisms, but a few species have acquired the ability to infect underground plant tissues. the predominant causal agent of potato scab worldwide is streptomyces scabies. the production of phytotoxins called thaxtomins is essential for the virulence of common scab-causing agen ... | 2009 | 19694949 |
| analysis of brassica rapa ests: gene discovery and expression patterns of ap2/erf family genes. | chinese cabbage (brassica rapa subsp. pekinensis) is among the most important vegetables and is widely cultivated in world. genes in the ap2/erf family encode transcriptional regulators that serve a variety of functions in the plants. expressed sequence tags (ests) are created by partially sequencing randomly isolated gene transcripts and have proved valuable in molecular biology. starting from the database with 142 947 ests of b. rapa, 62 putative ap2/erf family genes were identified by in sili ... | 2010 | 19701799 |
| expression of brd1, a plant defensin from brassica rapa, confers resistance against brown planthopper (nilaparvata lugens) in transgenic rices. | plant defensins are small (5-10 kda) basic peptides thought to be an important component of the defense pathway against fungal and/or bacterial pathogens. to understand the role of plant defensins in protecting plants against the brown planthopper, a type of insect herbivore, we isolated the brassica rapa defensin 1 (brd1) gene and introduced it into rice (oryza sativa l.) to produce stable transgenic plants. the brd1 protein is homologous to other plant defensins and contains both an n-terminal ... | 2009 | 19714315 |
| sequenced bac anchored reference genetic map that reconciles the ten individual chromosomes of brassica rapa. | in view of the immense value of brassica rapa in the fields of agriculture and molecular biology, the multinational brassica rapa genome sequencing project (brgsp) was launched in 2003 by five countries. the developing brgsp has valuable resources for the community, including a reference genetic map and seed bac sequences. although the initial b. rapa linkage map served as a reference for the brgsp, there was ambiguity in reconciling the linkage groups with the ten chromosomes of b. rapa. conseq ... | 2009 | 19751531 |
| brassica orthologs from banyuls belong to a small multigene family, which is involved in procyanidin accumulation in the seed. | as part of a research programme focused on flavonoid biosynthesis in the seed coat of brassica napus l. (oilseed rape), orthologs of the banyuls gene that encoded anthocyanidin reductase were cloned in b. napus as well as in the related species brassica rapa and brassica oleracea. b. napus genome contained four functional copies of ban, two originating from each diploid progenitor. amino acid sequences were highly conserved between the brassicaceae including b. napus, b. rapa, b. oleracea as wel ... | 2009 | 19760260 |
| a microsatellite (ssr) based linkage map of brassica rapa. | in the present study we describe the construction of a genetic linkage map for the brassica rapa (aa) genome that will act as a key resource in undertaking future structural and functional genomic studies in b. rapa. a f(2) mapping population consisting of 48 f(2) individual plants developed following hybridization of 2 inbred lines bathari mandi and ic 331817 was used to construct the map. the map comprises 53 ssr markers derived from 3 different public domain resources. nine linkage groups alo ... | 2009 | 19761878 |
| development of public immortal mapping populations, molecular markers and linkage maps for rapid cycling brassica rapa and b. oleracea. | publicly available genomic tools help researchers integrate information and make new discoveries. in this paper, we describe the development of immortal mapping populations of rapid cycling, self-compatible lines, molecular markers, and linkage maps for brassica rapa and b. oleracea and make the data and germplasm available to the brassica research community. the b. rapa population consists of 160 recombinant inbred (ri) lines derived from the cross of highly inbred lines of rapid cycling and ye ... | 2009 | 19784615 |
| ectopic expression of a chinese cabbage brargos gene in arabidopsis increases organ size. | chinese cabbage (brassica rapa l. ssp. pekinensis) is a common and economically important crop in asia. specific targets of plant breeding programs for cabbage have been improvement in yield, resistance to environment stresses, and nutrition quality by means of genetic manipulation. to obtain information on yield improvement applicable for the genetic engineering approach, we have attempted to dissect the molecular pathways that regulate organ size. we first isolated a putative homolog of argos ... | 2010 | 19789991 |
| a novel plant cysteine-rich peptide family conferring cadmium tolerance to yeast and plants. | we have identified a novel cdna clone, termed dccdt1, from digitaria ciliaris, that confers cadmium (cd)-tolerance to yeast (saccharomyces cerevisiae). the gene encodes a predicted peptide of 55 amino acid residues of which 15 (27.3%) are cysteine residues. we found that monocotyledonous plants possess multiple dccdt1 homologues, for example rice contains five dccdt1 homologues (designated oscdt1~5), whereas dicotyledonous plants, including arabidopsis thaliana, brassica rapa, poplar (populus tr ... | 2009 | 19816106 |
| genome-wide comparative analysis of the brassica rapa gene space reveals genome shrinkage and differential loss of duplicated genes after whole genome triplication. | brassica rapa is one of the most economically important vegetable crops worldwide. owing to its agronomic importance and phylogenetic position, b. rapa provides a crucial reference to understand polyploidy-related crop genome evolution. the high degree of sequence identity and remarkably conserved genome structure between arabidopsis and brassica genomes enables comparative tiling sequencing using arabidopsis sequences as references to select the counterpart regions in b. rapa, which is a strong ... | 2009 | 19821981 |
| genome-wide identification of nbs-encoding resistance genes in brassica rapa. | nucleotide-binding site (nbs)-encoding resistance genes are key plant disease-resistance genes and are abundant in plant genomes, comprising up to 2% of all genes. the availability of genome sequences from several plant models enables the identification and cloning of nbs-encoding genes from closely related species based on a comparative genomics approach. in this study, we used the genome sequence of brassica rapa to identify nbs-encoding genes in the brassica genome. we identified 92 non-redun ... | 2009 | 19838736 |
| cell wall invertase 4 is required for nectar production in arabidopsis. | to date, no genes have been reported to directly affect the de novo production of floral nectar. in an effort to identify genes involved in nectar production, the affymetrix((r)) ath1 genechip was previously used to examine global gene expression profiles in arabidopsis thaliana nectaries. one of the genes displaying highly enriched expression in nectaries was cell wall invertase 4 (atcwinv4, at2g36190), which encodes an enzyme that putatively catalyses the hydrolysis of sucrose into glucose and ... | 2009 | 19861655 |
| genetic load and transgenic mitigating genes in transgenic brassica rapa (field mustard) x brassica napus (oilseed rape) hybrid populations. | one theoretical explanation for the relatively poor performance of brassica rapa (weed) x brassica napus (crop) transgenic hybrids suggests that hybridization imparts a negative genetic load. consequently, in hybrids genetic load could overshadow any benefits of fitness enhancing transgenes and become the limiting factor in transgenic hybrid persistence. two types of genetic load were analyzed in this study: random/linkage-derived genetic load, and directly incorporated genetic load using a tran ... | 2009 | 19878583 |
| overexpression of a brassica rapa ngatha gene in arabidopsis thaliana negatively affects cell proliferation during lateral organ and root growth. | in an effort to elucidate biological functions of transcription factors of brassica rapa l. (ssp. pekinensis), an ngatha homolog, brnga1, that belongs to the b3-type transcription factor superfamily was identified and expressed in arabidopsis thaliana under the control of the cauliflower mosaic virus (camv) 35s promoter. arabidopsis plants overexpressing brnga1, named brnga1ox, displayed markedly reduced organ growth compared with the wild type: lateral organs, such as leaves, flowers and cotyle ... | 2009 | 19880400 |
| a brassica rapa linkage map of est-based snp markers for identification of candidate genes controlling flowering time and leaf morphological traits. | for identification of genes responsible for varietal differences in flowering time and leaf morphological traits, we constructed a linkage map of brassica rapa dna markers including 170 est-based markers, 12 ssr markers, and 59 bac sequence-based markers, of which 151 are single nucleotide polymorphism (snp) markers. by blastn, 223 markers were shown to have homologous regions in arabidopsis thaliana, and these homologous loci covered nearly the whole genome of a. thaliana. synteny analysis betw ... | 2009 | 19884167 |
| evolutionary trajectory of turnip mosaic virus populations adapting to a new host. | little is known about how some plant viruses establish successful cross-species transmission whilst others do not; the genetic basis for adaptation is largely unknown. this study investigated the genetic changes that occurred using the progeny of an infectious clone, p35tunos, derived from the turnip mosaic virus (tumv) uk 1 isolate, which has a brassica host type, but rarely infects raphanus systemically and then only asymptomatically. the genetic trajectory leading to viral adaptation was stud ... | 2010 | 19906944 |
| complexity of genome evolution by segmental rearrangement in brassica rapa revealed by sequence-level analysis. | the brassica species, related to arabidopsis thaliana, include an important group of crops and represent an excellent system for studying the evolutionary consequences of polyploidy. previous studies have led to a proposed structure for an ancestral karyotype and models for the evolution of the b. rapa genome by triplication and segmental rearrangement, but these have not been validated at the sequence level. | 2009 | 19922648 |
| sequence-level comparative analysis of the brassica napus genome around two stearoyl-acp desaturase loci. | we conducted a sequence-level comparative analyses, at the scale of complete bacterial artificial chromosome (bac) clones, between the genome of the most economically important brassica species, brassica napus (oilseed rape), and those of brassica rapa, the genome of which is currently being sequenced, and arabidopsis thaliana. we constructed a new b. napus bac library and identified and sequenced clones that contain homoeologous regions of the genome including stearoyl-acp desaturase-encoding g ... | 2010 | 19929877 |
| expression of a glutathione reductase from brassica rapa subsp. pekinensis enhanced cellular redox homeostasis by modulating antioxidant proteins in escherichia coli. | glutathione reductase (gr) is an enzyme that recycles a key cellular antioxidant molecule glutathione (gsh) from its oxidized form (gssg) thus maintaining cellular redox homeostasis. a recombinant plasmid to overexpress a gr of brassica rapa subsp. pekinensis (brgr) in e. coli bl21 (de3) was constructed using an expression vector pkm260. expression of the introduced gene was confirmed by semiquantitative rt-pcr, immunoblotting and enzyme assays. purification of the brgr protein was performed by ... | 2009 | 19936628 |
| [development of brassica rapa l. embryos under conditions of microgravity]. | results of comparative studies of the embryos of identical age formed under microgravity and ground laboratory control are presented. significant similarity of a rate of embryo development and degree of their differentiation in both variants has been shown. the single cases of the disturbances in embryo formation, and also a certain acceleration of endosperm development at the early stages of seed formation in microgravity are revealed. | 2009 | 19938622 |
| qtl mapping of clubroot resistance in radish (raphanus sativus l.). | a qtl analysis for clubroot resistance (cr) of radish was performed using an f(2) population derived from a crossing of a cr japanese radish and a clubroot-susceptible (cs) chinese radish. f(3) plants obtained by selfing of f(2) plants were used for the cr tests. the potted seedlings were inoculated and the symptom was evaluated 6 weeks thereafter. the mean disease indexes of the f(3) plants were used for the phenotype of the f(2). the results of two cr tests were analyzed for the presence of qt ... | 2010 | 20012934 |
| cross-species approaches to seed dormancy and germination: conservation and biodiversity of aba-regulated mechanisms and the brassicaceae dog1 genes. | seed dormancy is genetically determined with substantial environmental influence mediated, at least in part, by the plant hormone abscisic acid (aba). the aba-related transcription factor abi3/vp1 (aba insensitive3/viviparous1) is widespread among green plants. alternative splicing of its transcripts appears to be involved in regulating seed dormancy, but the role of abi3/vp1 goes beyond seeds and dormancy. in contrast, dog1 (delay of germination 1), a major quantitative trait gene more specific ... | 2010 | 20013031 |
| overexpression of brmorn, a novel 'membrane occupation and recognition nexus' motif protein gene from chinese cabbage, promotes vegetative growth and seed production in arabidopsis. | proteins that contain membrane occupation and recognition nexus (morn) motifs regulate various aspects of cellular metabolism by localizing proteins in different cellular organelles. the full-length brassica rapa morn motif protein (brmorn) cdna consists of 1,510 bp encoding 502 deduced amino acids with a predicted molecular mass of 55.8 kda and an isoelectric point of 9.72. brmorn is a novel protein composed of two n-terminal transmembrane helices and seven c-terminal morn motifs and it appears ... | 2010 | 20016940 |
| genome-wide discovery of dna polymorphism in brassica rapa. | single nucleotide polymorphisms (snps) and/or insertion/deletions (indels) are frequent sequence variations in the plant genome, which can be developed as molecular markers for genetic studies on crop improvement. the ongoing brassica rapa genome sequencing project has generated vast amounts of sequence data useful in genetic research. here, we report a genome-wide survey of dna polymorphisms in the b. rapa genome based on the 557 bacterial artificial clone sequences of b. rapa ssp. pekinensis c ... | 2010 | 20024583 |
| assessment of genetic diversity of accessions in brassicaceae genetic resources by frequency distribution analysis of s haplotypes. | plant genetic resources are important sources of genetic variation for improving crop varieties as breeding materials. conservation of such resources of allogamous species requires maintenance of the genetic diversity within each accession to avoid inbreeding depression and loss of rare alleles. for assessment of genetic diversity in the self-incompatibility locus (s locus), which is critically involved in the chance of mating, we developed a dot-blot genotyping method for self-incompatibility ( ... | 2010 | 20039015 |
| influence of urea fertilizer placement on nitrous oxide production from a silt loam soil. | urea placement in band or nests has been shown to enhance n use efficiency, but limited work has been done to assess its affect on n(2)o emissions. this study compared n(2)o emissions from urea prills applied to an amsterdam silt loam (fine-silty, mixed, superactive, frigid typic haplustolls) using broadcast, band, and nest placements. experiments were conducted in greenhouse pots (200 kg n ha(-1)) and in canola (brassica rapa l.) seeded fields using rates of 100 kg n ha(-1) (recommended) and 20 ... | 2013 | 20048299 |
| identification of qtls that control clubroot resistance in brassica oleracea and comparative analysis of clubroot resistance genes between b. rapa and b. oleracea. | to perform comparative studies of cr (clubroot resistance) loci in brassica oleracea and brassica rapa and to develop marker-assisted selection in b. oleracea, we constructed a b. oleracea map, including specific markers linked to cr genes of b. rapa. we also analyzed cr-qtls using the mean phenotypes of f(3) progenies from the cross of a resistant double-haploid line (anju) with a susceptible double-haploid line (gc). in the nine linkage groups obtained (o1-o9), the major qtl, pb-bo(anju)1, was ... | 2010 | 20069415 |