Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| studies on the introduction and mobility of the maize activator element in arabidopsis thaliana and daucus carota. | we have co-transformed carrot (daucus carota) and arabidopsis thaliana with an agrobacterium tumefaciens non-tumorigenic t-dna carrying the maize transposable element activator (ac) and an agrobacterium rhizogenes ri t-dna. we present evidence that the ac element transposes in transformed root or root-derived callus cultures of both species. we show that fertile plants can be regenerated from transformed, root-derived callus cultures of arabidopsis, demonstrating the utility of the ri plasmid fo ... | 1987 | 2832144 |
| presence of a chloroplast-like elongator trnamet gene in the mitochondrial genomes of soybean and arabidopsis thaliana. | the nucleotide sequence of elongator trna(met) genes from soybean chloroplast and mitochondria and arabidopsis thaliana mitochondria have been determined. the mitochondrial trna(met) genes from soybean and a. thaliana are identical, and they differ from the soybean chloroplast trna(met) gene by only four nucleotides. analysis of the flanking regions indicates that the mitochondrial trna(met) gene is not present on a large chloroplast dna insertion in the mitochondrial genome, but it suggests tha ... | 1988 | 2838185 |
| restriction fragment length polymorphism linkage map for arabidopsis thaliana. | we have constructed a restriction fragment length polymorphism linkage map for the nuclear genome of the flowering plant arabidopsis thaliana. the map, containing 90 randomly distributed molecular markers, is physically very dense; greater than 50% of the genome is within 1.9 centimorgans, or approximately 270 kilobase pairs, of the mapped dna fragments. the map was based on the meiotic segregation of markers in two different crosses. the restriction fragment length polymorphism linkage groups w ... | 1988 | 2901107 |
| isolation of genes expressed in specific tissues of arabidopsis thaliana by differential screening of a genomic library. | the small genome size of arabidopsis thaliana allows the isolation of genes expressed in specific tissues and under controlled conditions by the differential screening of a genomic library, as has been shown previously for yeast and drosophila. cdna probes, based on poly(a)+ mrna isolated from different arabidopsis organs, were used in colony hybridizations with 1145 randomly chosen genomic clones, representing 27,000 kb of arabidopsis dna. twenty percent of the clones containing low-copy-number ... | 1988 | 2901388 |
| an evolutionarily conserved protein binding sequence upstream of a plant light-regulated gene. | a protein factor, identified in nuclear extracts obtained from tomato (lycopersicon esculentum, solanaceae) and arabidopsis thaliana (brassicaceae) seedlings, specifically binds upstream sequences from the plant light-regulated gene family encoding the small subunit of ribulose 1,5-bisphosphate carboxylase/oxygenase (rbcs). rbcs upstream sequences from tomato, pea (pisum sativum, leguminosae), and arabidopsis are recognized by the factor. the factor recognition occurs via a short conserved seque ... | 1988 | 2902624 |
| a copia-like transposable element family in arabidopsis thaliana. | the fast generation time, small genome size and extensive genetic map of the crucifer arabidopsis thaliana have made it the subject of an increasing number of studies in plant molecular genetics. as transposable elements have greatly facilitated genetic analysis in a variety of species, we have attempted to identify an endogenous a. thaliana transposable element. we report here the discovery of a family of such elements, which we refer to as ta1 elements. sequence analysis of one such element sh ... | 1988 | 2904123 |
| nucleotide sequence of chloroplast cf1-atpase epsilon-subunit and elongator trnamet genes from arabidopsis thaliana. | 1988 | 2904134 | |
| a new locus (nia 1) in arabidopsis thaliana encoding nitrate reductase. | we have isolated two nitrate reductase genes and their corresponding cdnas from arabidopsis thaliana. sequences of the two cdnas, when compared to a sequence of a barley cdna clone, confirm their identity as nitrate reductase clones and show that they are closely related. the two genes have been mapped using restriction fragment length polymorphisms; gnr2 is close to the previously identified chl-3 locus and is probably identical to it, while gnr1 maps to a new locus (nia1) on chromosome 1, near ... | 1988 | 2905260 |
| an embryo-lethal mutant of arabidopsis thaliana is a biotin auxotroph. | lethal mutants have been used in a variety of animal systems to study the genetic control of morphogenesis and differentiation. abnormal development has been shown in some cases to be caused by defects in basic cellular processes. we describe in this report an embryo-lethal mutant of arabidopsis thaliana that can be rescued by the addition of biotin to arrested embryos cultured in vitro and to mutant plants grown in soil. mutant plants rescued in culture produced phenotypically normal seeds when ... | 1989 | 2909401 |
| molecular cloning and dna sequence of the arabidopsis thaliana alcohol dehydrogenase gene. | arabidopsis thaliana provides an excellent experimental plant system for molecular genetics because of its remarkably small genome size, near absence of dispersed middle repetitive dna, and short life cycle. we have cloned and determined the nucleotide sequence of a single-copy gene from a. thaliana likely to be the gene encoding alcohol dehydrogenase (adh; alcohol:nad+ oxidoreductase, ec 1.1.1.1). the gene was isolated from a random recombinant library by cross-hybridization with a maize adh1 g ... | 1986 | 2937058 |
| structure and expression of three light-harvesting chlorophyll a/b-binding protein genes in arabidopsis thaliana. | the genome of arabidopsis thaliana is exceedingly small, in part because it lacks the large middle repetitive dna component characteristic of other plants. in this paper we have characterized a member of the low copy dna component: the gene family for the light-harvesting chlorophyll a/b-protein. this gene family is unusual in that it contains far fewer members than the 7-16 coding sequences for this protein found in other plants. we used cross-hybridization with a lemna gene encoding a light-ha ... | 1986 | 3012462 |
| identification of upstream regulatory elements involved in the developmental expression of the arabidopsis thaliana cab1 gene. | we studied cis regulatory elements controlling the light-dependent organ-specific expression of arabidopsis thaliana chlorophyll a/b binding protein gene (cab1) by stably transforming tobacco plants using a tumor-inducing (ti) plasmid vector system. the results from the 5' and internal deletion analyses indicate that there are at least three cis-acting elements that are involved in the light-dependent developmental expression of cab1 gene. two such elements are located at the immediate upstream ... | 1988 | 3054877 |
| intron existence predated the divergence of eukaryotes and prokaryotes. | nucleotide sequences for the nuclear genes encoding chloroplast (gapa and gapb) and cytosolic (gapc) glyceraldehyde-3-phosphate dehydrogenases (gapdhs) from arabidopsis thaliana were determined. comparison of nucleotide sequences indicates that the divergence of chloroplast and cytosolic gapdh genes preceded the divergence of prokaryotes and eukaryotes. in addition, some intron-exon junctions are conserved among gapb, gapc, and chicken gapdh genes. these results provide evidence at the molecular ... | 1988 | 3055302 |
| specialized binary vector for plant transformation: expression of the arabidopsis thaliana ahas gene in nicotiana tabacum. | we constructed a cosmid vector, poca18, designed for transferring plant genomic libraries from agrobacterium tumefaciens to plants. clones from a genomic library of arabidopsis thaliana dna in poca 18 were propagated stably in both escherichia coli and a. tumefaciens. clones from the poca18 a. thaliana library were used to construct transgenic nicotiana tabacum plants; the dna inserts were transferred intact in 10 out of 16 transgenic n. tabacum plants examined but were partially deleted in six ... | 1988 | 3060849 |
| inhibitory effects of para-aminobenzoic acid on the formation and mutagenicity of n-nitroso compounds. | naturally occurring para-aminobenzoic acid (paba) inhibited the formation of n-methyl-n-nitrosourea (mnu) from a nitrosation mixture of n-methylurea and nitrite at ph 3. the suppressive effect of paba on the formation of mnu is higher than that of ascorbic acid. the presence of the mnu was assayed by its mutagenicity in a higher plant, arabidopsis thaliana. in addition, paba markedly reduced the mutagenicity of n-methyl-n'-nitro-n-nitrosoguanidine in a.thaliana, but had no or only a low inhibito ... | 2011 | 3062321 |
| molecular biology of plant growth and development. arabidopsis thaliana as an experimental system. | 1988 | 3077979 | |
| identification of gtp-binding proteins in the plasma membrane of higher plants. | antisera raised against a highly conserved amino acid sequence (g alpha-common peptide) of animal gs alpha, gi alpha, go alpha and gt alpha recognize, in plasma membranes of several higher plants, sets of proteins of mr = 37 and 31 kda (vicia faba), 36 and 31 kda (arabidopsis thaliana) and 38 and 34 kda (commelina communis). the a. thaliana proteins were solubilized and partially purified. they bind [35s]gtp gamma s with high affinity (apparent kd approximately 10 nm) and, with lower affinity, g ... | 1988 | 3142467 |
| two strands of dna are not equivalent as probes in hybridizations at low stringency. | only one of the two complementary strands of a restriction fragment hybridizes under low stringency conditions to a cloned arabidopsis thaliana genomic dna fragment. we propose that this effect is caused by the energetic nonequivalence of the two possible mismatched duplexes, resulting from the accumulation of mismatches and extrahelical bases. these mismatches will differ between the two duplexes. the choice of probe strand may therefore be important for the success of heterologous hybridizatio ... | 1988 | 3189816 |
| structure and expression of the u5 snrna gene of arabidopsis thaliana. conserved upstream sequence elements in plant u-rna genes. | we have previously characterized the u2 small nuclear (sn) rna gene family of arabidopsis thaliana. to find out the structural features of upstream and downstream non-coding regions that are shared by different u-rna genes in higher plants we have isolated the gene encoding a 125 nt-long u5 snrna of arabidopsis. activity of the cloned gene was demonstrated in stably transformed tobacco calli and by transient expression in transfected protoplasts of nicotiana plumbaginifolia. southern analysis in ... | 1988 | 3205713 |
| location, identity, amount and serial entry of chloroplast dna sequences in crucifer mitochondrial dnas. | southern blot hybridization techniques were used to examine the chloroplast dna (cpdna) sequences present in the mitochondrial dnas (mtdnas) of two brassica species (b. campestris and b. hirta), two closely related species belonging to the same tribe as brassica (raphanus sativa, crambe abyssinica), and two more distantly related species of crucifers (arabidopsis thaliana, capsella bursa-pastoris). the two brassica species and r. sativa contain roughly equal amounts (12-14 kb) of cpdna sequences ... | 2011 | 3224389 |
| plant expression vectors with the origin of replication of the w-type plasmid sa. | a new class of binary vectors has been constructed, containing the origin of replication of the w-type plasmid sa. these vectors are designed to express foreign genes in plants under control of the tr gene 2' promoter or the promoter of a light-inducible ribulose-1,5-bisphosphate carboxylase small subunit gene of arabidopsis thaliana. | 1988 | 3231690 |
| structure and expression of spinach leaf cdna encoding ribulosebisphosphate carboxylase/oxygenase activase. | ribulosebisphosphate carboxylase/oxygenase activase is a recently discovered enzyme that catalyzes the activation of ribulose-1,5-bisphosphate carboxylase/oxygenase ["rubisco"; ribulose-bisphosphate carboxylase; 3-phospho-d-glycerate carboxy-lyase (dimerizing), ec 4.1.1.39] in vivo. clones of rubisco activase cdna were isolated immunologically from spinach (spinacea oleracea l.) and arabidopsis thaliana libraries. sequence analysis of the spinach and arabidopsis cdnas identified consensus nucleo ... | 1988 | 3277181 |
| arabidopsis thaliana. | 1987 | 3327474 | |
| organic solvents inhibit the mutagenicity of promutagens dimethylnitrosamine and methylbutylnitrosamine in a higher plant arabidopsis thaliana. | the organic solvents dimethylsulphoxide (dmso), acetone, ethanol and dimethylformamide inhibited the mutagenic activity of the promutagens dimethylnitrosamine and methylbutylnitrosamine in a higher plant arabidopsis thaliana. in contrast, the direct-acting mutagens n-methyl-n'-nitro-n-nitrosoguanidine (mnng) and n-methyl-n-nitrosourea (mnu) were not affected by the organic solvents (with the exception of dmso). the effect of dmso on mnng- and mnu-mutagenesis is explained by its effect on the ph ... | 1986 | 3333268 |
| isolation of a higher eukaryotic telomere from arabidopsis thaliana. | we have developed a method for constructing genomic libraries enriched for telomeric dna sequences, enabling the isolation of telomeres from higher eukaryotic organisms with large chromosomes. the method was used to clone telomeric dna sequences from the flowering plant arabidopsis thaliana. a. thaliana telomeres are composed primarily of tandemly repeated blocks of the sequence 5'-ccctaaa-3' and are heterogeneous in size. genomic sequences that cross-hybridize at high stringency with a. thalian ... | 1988 | 3349525 |
| isolation and biochemical analysis of ethyl methanesulfonate-induced alcohol dehydrogenase null mutants of arabidopsis thaliana (l.) heynh. | several mutants have been isolated at the arabidopsis thaliana (l.) heynh. alcohol dehydrogenase (adh) gene locus using allyl alcohol selection on ethyl methanesulfonate (ems)-mutagenized seeds. eleven mutants were isolated in the adh1-a electrophoretic allele, and 21 in the adh1-s allele. these null mutants are characterized by the absence of measurable adh activity and genetic data showed that the mutations were confined to the adh1 gene locus of arabidopsis. eleven mutants in the adh1-a backg ... | 1988 | 3377754 |
| the beta-tubulin gene family of arabidopsis thaliana: preferential accumulation of the beta 1 transcript in roots. | the genome of arabidopsis thaliana (l.) heynh. was shown to contain a beta-tubulin gene family consisting of at least seven distinct genes and/or pseudogenes. genomic clones of five different beta-tubulin genes and/or pseudogenes have been isolated and partially characterized. the complete nucleotide sequence of one a. thaliana beta-tubulin gene, designated beta 1, has been determined. a comparison of the predicted amino acid sequence of the a. thaliana beta 1-tubulin with the predicted sequence ... | 1988 | 3384336 |
| transcriptional regulation of the arabidopsis thaliana chalcone synthase gene. | we have cloned an arabidopsis thaliana chalcone synthase (chs) gene on the basis of cross-hybridization with a petroselinum hortense chs cdna clone. the protein sequence deduced from the a. thaliana chs dna sequence is at least 85% homologous to the chs sequences from p. hortense, antirrhinum majus, and petunia hybrida. southern blot analysis indicated that chs is a single-copy gene in a. thaliana. high-intensity light treatment of a. thaliana plants for 24 h caused a 50-fold increase in chs enz ... | 1988 | 3386631 |
| sequence and nitrate regulation of the arabidopsis thaliana mrna encoding nitrate reductase, a metalloflavoprotein with three functional domains. | the sequence of nitrate reductase (ec 1.6.6.1) mrna from the plant arabidopsis thaliana has been determined. a 3.0-kilobase-long cdna was isolated from a lambda gt10 cdna library of arabidopsis leaf poly(a)+ rna. the cdna hybridized to a 3.2-kilobase mrna whose level increased 15-fold in response to treatment of the plant with nitrate. an open reading frame encoding a 917 amino acid protein was found in the sequence. this protein is very similar to tobacco nitrate reductase, being greater than 8 ... | 1988 | 3393528 |
| plastocyanin of arabidopsis thaliana; isolation and characterization of the gene and chloroplast import of the precursor protein. | a genomic clone encoding the plastocyanin precursor was isolated from an arabidopsis thaliana lambda embl3 library, with the help of a heterologous hybridization probe. the nucleotide (nt) sequence encoding the 171-amino acid precursor protein and 650 bp of the 5'-flanking region were determined. s1 nuclease mapping showed the arabidopsis coding region to be uninterrupted and the transcript to possess an untranslated leader of approx. 30 nt. the 5' region of the gene contains a 25-bp direct repe ... | 1988 | 3396882 |
| characterization of highly repetitive sequences of arabidopsis thaliana. | we have analyzed three classes of highly repetitive dna sequences of arabidopsis thaliana, composed of tamdemly repeated units of 180 bp, 500 bp, and 160 bp, respectively. the three families comprise approximately 2% of the arabidopsis genome and are the major component of the highly repetitive dna. the 500-bp element arose by duplication of one half of a 180-bp ancestor and insertion of a foreign segment between the two duplicated parts followed by amplification. the repeat elements contain occ ... | 1988 | 3405748 |
| nucleotide sequence of an actin gene from arabidopsis thaliana. | an 1830-bp genomic dna segment containing an arabidopsis thaliana actin gene, aac1, has been cloned and sequenced. the aac1 gene is present as a single-copy gene, but at least two other actin-like genes have been detected. comparison of the nucleotide sequence of aac1 with other cloned plant actin genes reveals four exons separated by three introns conservatively located in all plant actin genes. the deduced amino acid sequence has also been compared with actin protein sequences from other plant ... | 1988 | 3410320 |
| characterization of the alpha-tubulin gene family of arabidopsis thaliana. | the genome of arabidopsis thaliana (linnaeus) heynhold was shown to contain an alpha-tubulin gene family consisting of at least four genes and/or pseudogenes. the primary structure of a transcribed alpha-tubulin gene was determined. a comparison of the predicted amino acid sequence of the a. thaliana alpha-tubulin with the predicted amino acid sequences of alpha-tubulins of chlamydomonas reinhardtii, stylonychia lemnae, and homo spaiens reveals a high degree of homology; 90%, 87%, and 83% identi ... | 1987 | 3475704 |
| cloning of an arabidopsis thaliana gene encoding 5-enolpyruvylshikimate-3-phosphate synthase: sequence analysis and manipulation to obtain glyphosate-tolerant plants. | 5-enolpyruvylshikimate-3-phosphate synthase (epsps), the target of the herbicide glyphosate, catalyzes an essential step in the shikimate pathway common to aromatic amino acid biosynthesis. we have cloned an epsp synthase gene from arabidopsis thaliana by hybridization with a petunia cdna probe. the arabidopsis gene is highly homologous to the petunia gene within the mature enzyme but is only 23% homologous in the chloroplast transit peptide portion. the arabidopsis gene contains seven introns i ... | 1987 | 3481024 |
| a binary vector for transferring genomic libraries to plants. | the transformation of mutant plants with a complete recombinant library derived from wild-type dna followed by assay of transformed plants for complementation of the mutant phenotype is a promising method for the isolation of plant genes. the small genome of arabidopsis thaliana is a good candidate for attempting this so-called shotgun transformation. we present the properties of an a. thaliana genomic library cloned in a binary vector, pc22. this vector, designed to introduce genomic libraries ... | 1986 | 3534794 |
| the organic solvents acetone, ethanol and dimethylformamide potentiate the mutagenic activity of n-methyl-n'-nitro-n-nitrosoguanidine, but have no effect on the mutagenic potential of n-methyl-n-nitrosourea. | the frequency of recessive chlorophyll and embryonic lethals included by n-methyl-n'-nitro-n-nitrosoguanidine (mnng) in arabidopsis thaliana was markedly increased when exposure of the seeds to mnng (3 h) was carried out in the presence of 4-12% acetone, 4-16% ethanol or 8-32% dimethylformamide. the enhancement of mnng mutagenicity was proportional to the concentrations of these organic solvents. in contrast, neither of them, applied at the same conditions and doses, influenced the mutagenic act ... | 1987 | 3657835 |
| characterization of the genome of arabidopsis thaliana. | the small crucifer arabidopsis thaliana has many useful features as an experimental organism for the study of plant molecular biology. it has a four-week life-cycle, only five chromosomes and a genome size less than half that of drosophila. to characterize the dna sequence organization of this plant, we have randomly selected 50 recombinant lambda clones containing inserts with an average length of 12,800 base-pairs and analyzed their content of repetitive and unique dna by various genome blot, ... | 1986 | 3701864 |
| fluxes through the prokaryotic and eukaryotic pathways of lipid synthesis in the '16:3' plant arabidopsis thaliana. | the kinetics of [1-14c]acetate incorporation in arabidopsis thaliana l. (heyn) showed almost equal labelling of phosphatidylcholine (pc) and diacylgalactosylglycerol (dgg) at early times and the transfer of radioactivity from pc to dgg and diacyldigalactosylglycerol (ddg) at longer times. these kinetics demonstrated the parallel operation of the prokaryotic and eukaryotic pathways of lipid synthesis [roughan & slack (1982) annu. rev. plant physiol. 33, 97-132] in this tissue. at 2 h after the ap ... | 1986 | 3741384 |
| [experiments with growing plants on orbital stations saliut-5, saliut-6 and saliut-7]. | the experiments with air-dry crepis capillaris seeds flown on the spacecraft soyuz-16 and orbital stations salyut-5, salyut-6 and salyut-7 showed that the number of aberrant cells in the seedlings grown during flight (experimental) and after flight (flight control) was higher than that in the ground-based control. this number was greater in the experimental seedlings than in the flight controls. the plants arabidopsis thaliana grew from cotyledons to flowers during flight. the seeds developed po ... | 2006 | 3951179 |
| [study of descendants of "arabidopsis thaliana" plants treated with bacterial dna. i. presence and replication of exogenous dna]. | 1970 | 4098023 | |
| dna-mediated transformation of arabidopsis thaliana. | 1973 | 4133555 | |
| [activation of thymidine-3h incorporation in the desoxyribonucleic acid of arabidopsis thaliana l. heynh. induced by gamma radiations]. | 1966 | 4168223 | |
| [fate of exogenous dna during the growth and development of "arabidopsis thaliana"]. | 1969 | 4181702 | |
| [redistribution, at the time of blossoming, of exogenous dna absorbed by arabidopsis thaliana seeds]. | 1969 | 4186392 | |
| studies on the genetic activity of thymidine-base analogue in arabidopsis thaliana. | 2014 | 4240680 | |
| [biological effect of gamma-irradiation of arabidopsis thaliana (l) heynh seeds in relation to radiation intensity]. | 2015 | 4449994 | |
| dna-mediated genetic correction of thiamineless arabidopsis thaliana. | 1974 | 4833229 | |
| fate of exogenous dna in arabidopsis thaliana. translocation and integration. | 1971 | 4942550 | |
| radiation-induced polygenic mutation in arabidopsis thaliana. ii. analysis of lines selected for flowering time. | 1968 | 5254096 | |
| [mutagenic effect of laser radiation on the seeds of arabidopsis thaliana (l.) heynh]. | 1970 | 5474214 | |
| effect of temperature on survival of gamma-irradiated seeds of arabidopsis thaliana. | 1971 | 5549253 | |
| [the effect of gamma-irradiation of seed on the survival, growth, development and fruiting of arabidopsis thaliana (l.) heynh]. | 2001 | 5646740 | |
| [simultaneous effect of gamma irradiation and heat shock on the seed of arabidopsis thaliana (l.) heynh]. | 2008 | 5743193 | |
| isolation of nutritional mutants in arabidopsis thaliana. | 1964 | 5849557 | |
| genetic effects of ethyl methanesulfonate in combination with copper and zinc ions on arabidopsis thaliana. | 1965 | 5863906 | |
| killing and mutagenic efficiencies of heavy ionizing particles in arabidopsis thaliana. | 1967 | 6030572 | |
| inhibition of dimethylnitrosamine-induced mutagenesis in arabidopsis thaliana by diethyldithiocarbamate and carbon monoxide. | diethyldithiocarbamate and carbon monoxide markedly inhibited the frequency of embryonic and chlorophyll mutations induced by the metabolism-requiring mutagen dimethylnitrosamine in the higher plant arabidopsis thaliana. in contrast, the monoamine oxidase substrates, tryptamine, benzylamine and 2-phenylethylamine, had no such effect. the mutagenicity of a direct-acting mutagen, n-methyl-n-nitrosourea, was not altered by these inhibitors or substrates. | 1984 | 6318104 |
| polymorphism of alcohol dehydrogenase in arabidopsis thaliana (l.) heynh.: genetical and biochemical characterization. | zymograms of arabidopsis alcohol dehydrogenase (adh; ec 1.1.1.1) show a unique anodal migrating band. three electrophoretic variants were identified among geographical races and designated slow (s), fast (f), and superfast (a), according to their mobility on tris-citrate starch gels. in plants adh activity is confined mainly to pollen, seeds, and grains and rapidly declines during the germination process. in callus and suspension cultures, growing on media containing 2,4-d, adh appeared as one o ... | 1984 | 6393951 |
| mutants of the cruciferous plant arabidopsis thaliana lacking glycine decarboxylase activity. | a mutant of arabidopsis thaliana (l.) heyn. (a small plant in the crucifer family) that lacks glycine decarboxylase activity owing to a recessive nuclear mutation has been isolated on the basis of a growth requirement for high concentrations of atmospheric co2. mitochondria isolated from leaves of the mutant did not exhibit glycine-dependent o2 consumption, did not release 14co2 from [14c]glycine, and did not catalyse the glycine-bicarbonate exchange reaction that is considered to be the first p ... | 1982 | 6807291 |
| ems- and radiation-induced mutation frequencies at individual loci in arabidopsis thaliana (l.) heynh. | average mutation frequencies per locus per dose of mutagen - ems, x-rays and fast neutrons - were estimated from 15 different loci of arabidopsis thaliana. these loci relate to clearly distinguishable phenotypic mutant groups, each affected by a limited number of loci. there were significant differences in mutation rate between individual loci. the proposition of a linear relationship between dna content and mutation frequency per locus is rejected. | 1982 | 7062928 |
| mutants of arabidopsis thaliana with altered responses to auxins and gravity. | auxin-resistant mutants of arabidopsis have been induced and isolated by screening for survivors on a medium containing the herbicide 2,4-d. thirty independently arisen mutants have been isolated in this way and one of them, p 83, has been investigated in detail. when wild type and p 83 are compared in concentration/response curves, where the response is the inhibition of root growth, the ed50 values of the auxins, 2,4-d and iaa, are 14-fold higher for the mutant. the mutant also responds differ ... | 1980 | 7247923 |
| [structural and functional characteristics of the photosynthetic apparatus of the mutants arabidopsis thaliana (l.) heynh]. | the mutants arabidopsis thaliana triplex and 58/15 with a high and low content of green pigments, respectively, and a higher rate of photosynthesis in comparison with the control form of enkheim were studied. the significant differences in the structural organization of chloroplasts of both mutants were revealed. the two mutants are also characterized by a higher rate of ferricyanide reduction in the hill reaction and atp production via photophosphorylation. it was shown that mg2+, nh4cl and (ad ... | 1980 | 7378506 |
| "arabidobrassica": evidence for intergeneric somatic hybrid nature from electron microscopic morphometry of chromatin. | the new method of species and hybrid characterization and determination by quantitative morphometry of chromatin texture in ultra-thin sections of nuclei was applied to the somatic hybrid "arabidobrassica" and its parents, arabidopsis thaliana and brassica campestris. the somatic hybrid exhibits the average amount of condensed chromatin, if compared to the proportion of condensed chromatin in nuclei of the parent species. this result corresponds to similar findings in natural allopolyploids and ... | 1980 | 7398664 |
| arabidopsis thaliana tryptophan synthase alpha: gene cloning, expression, and subunit interaction. | the tryptophan synthase alpha subunit catalyzes the conversion of indole-3-glycerolphosphate to indole, the penultimate reaction in the biosynthesis of the essential amino acid tryptophan. a cdna encoding arabidopsis thaliana tryptophan synthase alpha(tsa1) was isolated by complementation of an escherichia coli delta trpa mutation and by polymerase chain reaction amplification from a cdna library using degenerate primers. a tsa1 genomic clone was also isolated and 5 kb of the dna sequence determ ... | 1995 | 7476868 |
| the tef1 box, a ubiquitous cis-acting element involved in the activation of plant genes that are highly expressed in cycling cells. | in arabidopsis thaliana, the tef1 box is a cis-acting promoter element of the ef-1 alpha a1 gene involved in the activation of transcription in meristematic tissues. the initiation of root calli in transgenic arabidopsis by 2,4-d shows that the tef1-dependent expression of the gus reporter gene is not restricted to meristematic regions but involves all of the cycling cells. hybridization experiments conducted using arabidopsis cdna clones organized in a dense array on filters, and cdna probes pr ... | 1995 | 7476873 |
| cell fate in the arabidopsis root meristem determined by directional signalling. | postembryonic development in plants is achieved by apical meristems. surgical studies and clonal analysis have revealed indirectly that cells in shoot meristems have no predictable destiny and that position is likely to play a role in the acquisition of cell identity. in contrast to animal systems, there has been no direct evidence for inductive signalling in plants until now. here we present evidence for such signalling using laser ablation of cells in the root meristem of arabidopsis thaliana. ... | 1995 | 7477287 |
| characterization of arabidopsis thaliana cdnas that render yeasts tolerant toward the thiol-oxidizing drug diamide. | diamide oxidizes cellular thiols and induces oxidative stress. to isolate plant genes which may, when overexpressed, increase tolerance of plants toward oxidative damage, an in vivo diamide tolerance screening in yeasts was used. an arabidopsis cdna library in a yeast expression vector was used to transform a yeast strain with intact antioxidant defense. cells from approximately 10(5) primary transformants were selected for resistance to diamide. three arabidopsis cdnas which confer diamide tole ... | 1995 | 7479844 |
| modification of the substrate specificity of an acyl-acyl carrier protein thioesterase by protein engineering. | the plant acyl-acyl carrier protein (acp) thioesterases (tes) are of biochemical interest because of their roles in fatty acid synthesis and their utilities in the bioengineering of plant seed oils. when the fatb1 cdna encoding a 12:0-acp te (uc fatb1) from california bay, umbellularia californica (uc) was expressed in escherichia coli and in developing oilseeds of the plants arabidopsis thaliana and brassica napus, large amounts of laurate (12:0) and small amounts of myristate (14:0) were accum ... | 1995 | 7479856 |
| the genome of arabidopsis thaliana. | arabidopsis thaliana is a small flowering plant that is a member of the family cruciferae. it has many characteristics--diploid genetics, rapid growth cycle, relatively low repetitive dna content, and small genome size--that recommend it as the model for a plant genome project. the current status of the genetic and physical maps, as well as efforts to sequence the genome, are presented. examples are given of genes isolated by using map-based cloning. the importance of the arabidopsis project for ... | 1995 | 7479893 |
| insect cell expression of recombinant imidazoleglycerolphosphate dehydratase of arabidopsis and wheat and inhibition by triazole herbicides. | imidazoleglycerolphosphate dehydratase (igpd; ec 4.2.1.19), which is involved in the histidine biosynthetic pathway of arabidopsis thaliana and wheat (triticum aestivum), has been expressed in insect cells using the baculovirus expression vector system. n-terminal amino acid sequencing indicated that recombinant igpds (rigpds) were produced as mature forms via nonspecific proteolytic cleavages in the putative transit peptide region. the wheat rigpd contained one mn atom per subunit, and the mn w ... | 1995 | 7480319 |
| light-dependent and tissue-specific expression of the h-protein of the glycine decarboxylase complex. | glycine decarboxylase is a mitochondrial enzyme complex, which is the site of photorespiratory co2 and nh3 release. although the proteins that constitute the complex are located within the mitochondria, because of their intimate association with photosynthesis their expression is controlled by light. comparisons of the kinetics of mrna accumulation between the small subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase and the h-protein of glycine decarboxylase during the greening of etiola ... | 1995 | 7480320 |
| differential accumulation of antioxidant mrnas in arabidopsis thaliana exposed to ozone. | antioxidant isoenzymes function to eliminate free radicals and are localized to several different subcellular compartments within the plant cell. in arabidopsis thaliana exposed to ozone (o3), we have monitored the accumulation of mrnas encoding both cytosolic and chloroplastic antioxidant isoenzymes. two different o3 exposure protocols yielded similar results. upon o3 exposure, the steady-state levels of three mrnas encoding cytosolic antioxidant isoenzymes (ascorbate peroxidase, copper/zinc su ... | 1995 | 7480322 |
| evidence that plant k+ channel proteins have two different types of subunits. | plant k+ channel proteins have been previously characterized as tetramers of membrane-spanning alpha subunit polypeptides. recent studies have identified a 39-kd, hydrophilic polypeptide that is a structural component of purified animal k+ channel proteins. we have cloned and sequenced an arabidopsis thaliana cdna encoding a 38.4-kd polypeptide that has a sequence homologous to the animal k+ channel beta subunit. southern and northern analyses indicate the presence of a gene encoding this cdna i ... | 1995 | 7480329 |
| expression of an arabidopsis potassium channel gene in guard cells. | the arabidopsis thaliana kat1 cdna encodes a voltage-gated inward-rectifying k+ channel. a kat1 genomic dna clone was isolated and sequenced, and a 5' promoter and coding sequences containing eight introns were identified. reporter gene analysis of transgenic plants containing the kat1 promoter fused to bacterial beta-glucuronidase showed robust beta-glucuronidase activity primarily in guard cells. | 1995 | 7480337 |
| molecular cloning and characterization of the cdna coding for the biotin-containing subunit of the chloroplastic acetyl-coenzyme a carboxylase. | we report the molecular cloning and sequence of the cdna coding for the biotin-containing subunit of the chloroplastic acetylcoenzyme a (coa) carboxylase (accase) of arabidopsis thaliana (cac1). the 3' end of the cac1 sequence, coding for a peptide of 94 amino acids, which includes a putative biotinylation motif, was expressed in escherichia coli as a glutathione-s-transferase (gst) fusion protein. the resulting gst-cac1 fusion protein was biotinylated in vivo, indicating that cac1 codes for a b ... | 1995 | 7480350 |
| multiple isoforms of arabidopsis casein kinase i combine conserved catalytic domains with variable carboxyl-terminal extensions. | three cdna clones encoding isoforms of casein kinase i (cki) were isolated from arabidopsis thaliana. one full-length clone, designated cki1, contained an open reading frame of 1371 bp encoding a protein of 51,949 d with an isoelectric point of 9.7. in addition to the highly conserved catalytic domain (of about 300 amino acids), the arabidopsis cki isoforms contain 150 to 180 amino acid carboxyl-terminal extensions, which show among themselves a lower level of sequence conservation. these extens ... | 1995 | 7480353 |
| adenosine-5'-triphosphate-sulfurylase from arabidopsis thaliana and escherichia coli are functionally equivalent but structurally and kinetically divergent: nucleotide sequence of two adenosine-5'-triphosphate-sulfurylase cdnas from arabidopsis thaliana and analysis of a recombinant enzyme. | atp-sulfurylase, the first enzyme of sulfate assimilation, catalyzes the formation of adenosine-5'-phosphosulfate from atp and sulfate. here we report that the higher plant, arabidopsis thaliana, contains a three-member, expressed gene family encoding plastid localized forms of atp sulfurylase. three cdnas from a. thaliana, designated aps1, aps2, and aps3, were isolated by their ability to functionally complement a met3 (atp sulfurylase) mutant strain of saccharomyces cerevisiae (yeast). the nuc ... | 1995 | 7487067 |
| [the genetic consequences of the radioactive contamination of populations of arabidopsis thaliana growing in a 30-kilometer area of the accident at the chernobyl atomic electric power station]. | annually during six years since 1986 dynamics of the mutation load in arabidopsis populations was studied. frequency of the embryonic lethal mutations was studied with muller embryonic test. first two years after the accident an increase of mutation load in arabidopsis populations was observed at all levels of radioactive contamination. in the areas with contamination up to 10 mr/h the mutation load decreased to the control level by 1990. in the areas with contamination up to 130 mr/h the mutati ... | 1995 | 7489102 |
| transgene inactivation in arabidopsis thaliana. | 1995 | 7493495 | |
| the use of an alternative promoter in the arabidopsis thaliana hmg1 gene generates an mrna that encodes a novel 3-hydroxy-3-methylglutaryl coenzyme a reductase isoform with an extended n-terminal region. | the enzyme 3-hydroxy-3-methylglutaryl-coa reductase (hmgr, ec 1.1.1.34) catalyses the synthesis of mevalonate, the committed precursor of the great variety of isoprenoid compounds and derivatives synthesized in higher plants. it has previously been reported that arabidopsis thaliana contains two differentially expressed genes, hmg1 and hmg2, that encode two hmgr isoforms (hmgr1 and hmgr2, respectively). this paper reports the characterization of a novel hmgr mrna (hmgr1l mrna) derived from the h ... | 1995 | 7496400 |
| sequence-based identification of t-dna insertion mutations in arabidopsis: actin mutants act2-1 and act4-1. | a method is presented to facilitate the isolation of mutations in plant genes, which requires knowledge of the target gene or protein sequence, and is independent of mutant phenotype. the polymerase chain reaction was used to amplify the junctions between a t-dna insert and the gene of interest from pools of mutant plant lines. the approach was used to identify mutations in arabidopsis thaliana actin genes. the arabidopsis genome encodes 10 actins in six ancient subclasses each with distinct exp ... | 1995 | 7496405 |
| radiation-sensitive mutants of arabidopsis thaliana. | five arabidopsis mutants have been isolated on the basis of hypersensitivity of leaf tissue to uv light. for each mutant, the uv-hypersensitive phenotype (uvh) was inherited as a single recessive mendelian trait. in addition, each uvh mutant represented a separate complementation group. three of the mutations producing the uv hypersensitive phenotype have been mapped relative to either genetic markers or physical microsatellite polymorphisms. locus uvh1 is linked to nga76 on chromosome 5, uvh3 t ... | 1995 | 7498749 |
| molecular characterization of a putative arabidopsis thaliana copper transporter and its yeast homologue. | at the molecular level, little is known about the transport of copper across plant membranes. we have isolated an arabidopsis thaliana cdna by complementation of a mutant (ctr1-3) of saccharomyces cerevisiae defective in high affinity copper uptake. this cdna codes for a highly hydrophobic protein (copt1) of 169 amino acid residues and with three putative transmembrane domains. most noteworthy, the first 44 residues display significant homology to the methionine- and histidine-rich copper bindin ... | 1995 | 7499355 |
| conserved initiator proteins in eukaryotes. | the origin recognition complex (orc), a multisubunit protein identified in saccharomyces cerevisiae, binds to chromosomal replicators and is required for the initiation of cellular dna replication. complementary dnas (cdnas) encoding proteins related to the two largest subunits of orc were cloned from various eukaryotes. the cdnas encoding proteins related to s. cerevisiae orc1p were cloned from the budding yeast kluyveromyces lactis, the fission yeast schizosaccharomyces pombe, and human cells. ... | 1995 | 7502077 |
| characterization of cdna for a dehydration-inducible gene that encodes a clp a, b-like protein in arabidopsis thaliana l. | sequence was obtained from a cdna clone, designated erd1, isolated from a cdna library of 1-hour-dehydrated plants of arabidopsis thaliana l. the clone (3150 bp) contains an open reading frame of 946 amino acid residues with greater than 34% sequence identity to the regulatory subunit of the clp atp-dependent protease in escherichia coli and contains a putative chloroplast-targeting signal at the n-terminus. southern blot analysis suggested the presence of additional erd1-related genes in a. tha ... | 1993 | 7504470 |
| isolation of an arabidopsis thaliana gene encoding cycloartenol synthase by functional expression in a yeast mutant lacking lanosterol synthase by the use of a chromatographic screen. | whereas vertebrates and fungi synthesize sterols from epoxysqualene through the intermediate lanosterol, plants cyclize epoxysqualene to cycloartenol as the initial sterol. we report the cloning and characterization of cas1, an arabidopsis thaliana gene encoding cycloartenol synthase [(s)-2,3-epoxysqualene mutase (cyclizing, cycloartenol forming), ec 5.4.99.8]. a yeast mutant lacking lanosterol synthase [(s)-2,3-epoxysqualene mutase (cyclizing, lanosterol forming), ec 5.4.99.7] was transformed w ... | 1993 | 7505443 |
| an arabidopsis thaliana lipoxygenase gene can be induced by pathogens, abscisic acid, and methyl jasmonate. | we isolated and characterized a 2.8-kb, full-length, arabidopsis thaliana cdna clone encoding a lipoxygenase. dna sequence analysis showed that the deduced amino acid sequence of the arabidopsis protein is 72 to 78% similar to that of legume seed lipoxygenases. dna blot analysis indicated that arabidopsis contains a single gene, lox1, with appreciable homology to the cdna clone. rna blot analysis showed that the lox1 gene is expressed in arabidopsis leaves, roots, inflorescences, and young seedl ... | 1993 | 7506426 |
| conversion of perianth into reproductive organs by ectopic expression of the tobacco floral homeotic gene nag1. | mutations in the agamous (ag) gene of arabidopsis thaliana result in the conversion of reproductive organs, stamens and carpels, into perianth organs, sepals and petals. we have isolated and characterized the putative ag gene from nicotiana tabacum, nag1, whose deduced protein product shares 73% identical amino acid residues with the arabidopsis ag gene product. rna tissue in situ hybridizations show that nag1 rna accumulates early in tobacco flower development in the region of the floral merist ... | 1993 | 7507255 |
| the arabidopsis thaliana apurinic endonuclease arp reduces human transcription factors fos and jun. | an arabidopsis thaliana cdna encoding an analogue, referred to as arp for apurinic endonuclease-redox protein, of the human redox factor ref has been cloned. arp stimulates in vitro dna-binding activity of the human transcription factor jun and fos by the reduction of a cysteine residue located in the dna-binding domain. based on amino acid sequence homology, this redox activity is probably confined to the small internal domain of the arp protein. in analogy to ref, we show that the arabidopsis ... | 1994 | 7512729 |
| genes encoding glycine-rich arabidopsis thaliana proteins with rna-binding motifs are influenced by cold treatment and an endogenous circadian rhythm. | we have characterized the expression of two members of a class of arabidopsis thaliana glycine-rich, putative rna-binding proteins that we denote ccr1 and ccr2. southern blot analysis indicates that ccr1 and ccr2 are members of a small gene family. both ccr1 and ccr2 mrna levels were influenced by a circadian rhythm that has an unusual phase for plants, with maximal accumulation at 6:00 pm and minimal accumulation at 10:00 am. the level of ccr1 protein, however, remained relatively constant thro ... | 1994 | 7513083 |
| structure of the gene for an auxin-binding protein and a gene for 7sl rna from arabidopsis thaliana. | a genomic clone encoding an auxin-binding protein (abp) from the endoplasmic reticulum was isolated from arabidopsis thaliana. the abp gene consisted of 5 exons and 4 introns and encoded a polypeptide of 198 residues. a gene encoding the 7sl rna of the signal recognition particle was located downstream of the abp gene. | 1993 | 7517789 |
| two related low-temperature-inducible genes of arabidopsis encode proteins showing high homology to 14-3-3 proteins, a family of putative kinase regulators. | we have isolated two rare cold-inducible (rci1 and rci2) cdnas by screening a cdna library prepared from cold-acclimated etiolated seedlings of arabidopsis thaliana with a subtracted probe. rna-blot hybridizations revealed that the expression of both rci1 and rci2 genes is induced by low temperature independently of the plant organ or the developmental stage considered. however, rci1 mrna accumulates faster and at higher levels than the rci2 one indicating that these genes have differential resp ... | 1994 | 7520301 |
| characterization and expression of two cdnas encoding carbonic anhydrase in arabidopsis thaliana. | two distinct cdna clones encoding carbonic anhydrase (ca) were isolated from an arabidopsis thaliana lambda yes library. one of these clones, ca1, encodes a 36.1-kd polypeptide and is essentially the same as a previously reported arabidopsis ca cdna (c.a. raines, p.r. horsnell, c. holder, j.c. lloyd [1992] plant mol biol 20: 1143-1148). comparison of the derived amino acid sequence from this clone with other plant cas suggests the presence of a chloroplastic transit peptide, which, when cleaved, ... | 1994 | 7520589 |
| olomoucine, an inhibitor of the cdc2/cdk2 kinases activity, blocks plant cells at the g1 to s and g2 to m cell cycle transitions. | the cdc2/cdk2 protein kinases play key roles in the cell cycle at two control points: the g1/s transition and the entry into mitosis. olomoucine, a specific inhibitor of these kinases, was tested in two plant cell systems: petunia mesophyll protoplasts induced to divide and arabidopsis thaliana cell suspension cultures. the cell cycle status was analysed from dna histograms or through continuous labelling of cells with 5-bromodeoxyuridine (brdurd) followed by double staining with bis-benzimide ( ... | 1994 | 7523194 |
| environmental monitoring for genotoxicity with plant systems. an introduction and study design. | under the sponsorship of the international programme on chemical safety (ipcs), 17 laboratories from diverse regions of the world participated in evaluating the utility of four plant bioassays for detecting genetic hazards of environmental chemicals. the bioassays included in this collaborative study were: arabidopsis thaliana embryo and chlorophyll assay and tradescantia stamen hair assay, tradescantia paludosa micronucleus assay and vicia faba root tip assay. four to six laboratories participa ... | 1994 | 7523889 |
| the present status of higher plant bioassays for the detection of environmental mutagens. | higher plants provide valuable genetic assay systems for screening and monitoring environmental pollutants. they are now recognized as excellent indicators of cytogenetic and mutagenic effects of environmental chemicals and are applicable for the detection of environmental mutagens both indoor and outdoor. comparisons between plant and nonplant genetic assay systems indicate that higher plant genetic assays have a high sensitivity (i.e. few false negatives). two assays which are considered ideal ... | 1994 | 7523890 |
| arabidopsis assay for mutagenicity. | four laboratories, two in the czech republic (brno and prague) and two in the cis (moscow and duschanbe), participated in the international programme on chemical safety's (ipcs) collaborative study to evaluate the utility of the most commonly used plant test systems, including the arabidopsis thaliana assay, for assessing the mutagenic potential of environmental agents. out of the five compounds evaluated in the arabidopsis assay, three compounds, i.e., ethyl methanesulfonate, n-methyl-n-nitroso ... | 1994 | 7523895 |
| characterization of some major identity elements in plant alanine and phenylalanine transfer rnas. | alanine and phenylalanine trna sequences were amplified by pcr from arabidopsis thaliana nuclear dna using degenerate oligonucleotides which introduced specific mutations into the acceptor stem. the aminoacylation of t7 rna polymerase transcripts of these sequences was investigated in vitro using partially purified bean alanyl- or phenylalanyl-trna synthetase. in parallel, the in vivo activity of amber suppressor derivatives of these trnas was investigated in transient expression assays in tobac ... | 1994 | 7532029 |
| the blue light-responsive athh2 gene of arabidopsis thaliana is primarily expressed in expanding as well as in differentiating cells and encodes a putative channel protein of the plasmalemma. | according to our previous studies the arabidopsis gene athh2 which is inducible by blue light and phytohormones codes for an intrinsic membrane protein. it bears a resemblance to several distinct channel proteins of plant and animal species classified as the mip/nod-26/glpf family. in the present study biochemical analyses and electron microscopic immunochemistry were used to elucidate the subcellular location of the athh2 protein. the results clearly demonstrate that it is an exclusive constitu ... | 1995 | 7534555 |
| role of the phytotoxin coronatine in the infection of arabidopsis thaliana by pseudomonas syringae pv. tomato. | the role of the phytotoxin coronatine in the virulence of pseudomonas syringae pv. tomato in arabidopsis thaliana was evaluated by comparing symptom development, in planta bacterial multiplication, and the induction of defense-related genes in arabidopsis plants inoculated with the coronatine-producing (cor+) p. s. pv. tomato strain dc3000 and the coronatine-defective (cor-) strain dc3661 by either infiltration or dipping methods. the cor+ strain, p. s. pv. tomato dc3000, caused severe disease s ... | 1995 | 7539639 |