Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| an algorithm for determining the conformation of polypeptide segments in proteins by systematic search. | the feasibility of determining the conformation of segments of a polypeptide chain up to six residues in length in globular proteins by means of a systematic search through the possible conformations has been investigated. trial conformations are generated by using representative sets of phi, psi, and chi angles that have been derived from an examination of the distributions of these angles in refined protein structures. a set of filters based on simple rules that protein structures obey is used ... | 1986 | 3130622 |
| [isolation and various properties of the lytic enzyme preparation from streptomyces griseus]. | certain optimal parameters were determined for production of a pure enzyme preparation of the giox type from s. griseus by precipitating the enzymes from concentrated fermentation broth filtrate. antibacterial spectrum of the preparation is comparatively narrow. still, it showed high lytic activity against streptococci and staphylococci. investigation of the preparation with the electrofocusing technique revealed that the main lysing component was a protein fraction with its isoelectric point wi ... | 1988 | 3132114 |
| [selection of strains of streptomyces griseus kr., the producer of a streptothricin antibiotic grisin, using the method of protoplast fusion]. | the effect of protoplasting on antibiotic activity of the grisin-producing organism was shown. high frequency of grn- mutants after strain vg307f protoplasting and no capacity in these mutants for reversion to the initial grn+ phenotype were shown. the reversion frequency was less than 10(-8). moreover, it was shown that all the grn- mutants lost their stability (grnr) to the effect of their own antibiotic. with respect to strain vg212 there was noted a significant increase in the number of both ... | 1988 | 3132116 |
| [a new subspecies of streptomyces griseus]. | 1988 | 3133886 | |
| refined crystal structure of streptomyces griseus trypsin at 1.7 a resolution. | streptomyces griseus trypsin (sgt) is a bacterial serine proteinase that is more homologous to mammalian than to other bacterial enzymes. the structure of sgt has been solved primarily by molecular replacement, though some low-resolution phase information was supplied by heavy-atom derivatives. the mammalian pancreatic serine proteinases bovine trypsin (bt) and alpha-chymotrypsin (cht) were used as molecular replacement models. because these proteins have low homology with sgt compared to the ma ... | 1988 | 3135412 |
| strain- and species-specific distribution of the streptomycin gene cluster and kan-related sequences in streptomyces griseus. | the streptomycin (sm) gene cluster was investigated for its distribution in streptomycetes by southern hybridization using nick-translated dna probes, which were isolated from the sm-6-phosphotransferase (sph) and amidinotransferase (adt) regions of the sm gene cluster of streptomyces griseus ss-1198. bgl ii-digested genomic dnas from sm-producing strains of s. griseus yielded the same size fragment (7.0 kb) which hybridized to both the sph and adt probes as expected from the restriction endonuc ... | 1988 | 3139605 |
| [creation of a polyenzyme composition of proteases from streptomyces griseus and acremonium chrysogenum]. | proteolytic enzymes of microorganisms have been studied for the possibility to create their polyenzymic composition in order to rise a degree of protein hydrolysis and to lower the process duration. optimal action conditions are selected and a hydrolysis of a number of globular and fibrillar proteins is conducted by a polyenzymic system of streptomyces griseus and acremonium chrysogenum proteases. | 1988 | 3142128 |
| xenobiotic oxidation by cytochrome p-450-enriched extracts of streptomyces griseus. | crude extracts of streptomyces griseus grown on soybean flour-enriched medium contain high levels of cytochrome p-450. the cytochrome p-450-enriched fractions, obtained by ammonium sulfate fractionation (30-50% saturation), catalyze the nadph-dependent oxidation of a variety of xenobiotics when complemented with both spinach ferredoxin:nadp+ oxidoreductase and spinach ferredoxin. reactions observed are aromatic, benzylic and alicyclic hydroxylations, o-dealkylation, non-aromatic double bond epox ... | 1988 | 3144975 |
| [hybridization of a-factor deficient mutants of streptomyces griseus by means of fusion of protoplasts]. | characteristics of 6 a-factor deficient mutants of s. griseus are presented. the common feature of the mutants was impairment of sporulation, formation of aerial mycelium and streptomycin synthesis. pair-by-pair hybridization of the mutants was performed with protoplast fusion followed by regeneration. 9 pair couplings of the mutants were performed. in 3 of them sporulating recombinants were detected. the antibiotic production level in 70 hybrids was different and ranged from 0 to 1700 microgram ... | 1988 | 3145721 |
| chitosanase from streptomyces griseus. | 1988 | 3147359 | |
| purification and characterization of an acidic amino acid specific endopeptidase of streptomyces griseus obtained from a commercial preparation (pronase). | a protease was purified 163-fold from pronase, a commercial product from culture filtrate of streptomyces griseus, by a series of column chromatographies on cm-toyopearl (fractogel), sephadex g-50, hydroxyapatite, and z-gly-d-phe-ah-sepharose 4b using boc-ala-ala-pro-glu-pna as a substrate. the final preparation was homogeneous by polyacrylamide gel electrophoresis (page), sodium dodecyl sulfate polyacrylamide gel electrophoresis (sds-page), and gel isoelectric focusing. studies on the substrate ... | 1988 | 3149277 |
| [isolation of auxotrophic mutants under the action of n-methyl-n'-nitro-n-nitrosoguanidine and uv radiation and the mapping of new loci on the streptomyces griseus chromosome by protoplast fusion]. | 1987 | 3150502 | |
| purification and partial characterization of multiple bromoperoxidases from streptomyces griseus. | the presence of multiple bromoperoxidases in extracts of streptomyces griseus tü 6 was detected. the enzyme pattern varied with the age of the culture. a haem-type bromoperoxidase (bpo 2) was always present. additionally three nonhaem-type bromoperoxidases (bpo 1a, 1b and 3) were detected and purified to homogeneity. the mr of non-denatured bpo 1a was 70,000 +/- 10,000 and those of bpo 1b and 3 were 90,000 +/- 5000. bpo 1a and 1b were dimers with subunit mr values of 34,000 and 43,000, respectiv ... | 1988 | 3151989 |
| two-dimensional nmr studies of kazal proteinase inhibitors. 1. sequence-specific assignments and secondary structure of turkey ovomucoid third domain. | two-dimensional proton nmr experiments have been used to sequentially assign resonances to all of the peptide backbone protons of turkey ovomucoid third domain (omtky3) except those of the n-terminal alpha-amino group whose signal was not resolved owing to exchange with the solvent. assignments also have been made for more than 80% of the side-chain protons. two-dimensional chemical shift correlated spectroscopy (cosy), relayed coherence transfer spectroscopy (relay), and two-dimensional homonuc ... | 1988 | 3164216 |
| 16-deethylindanomycin (a83094a), a novel pyrrole-ether antibiotic produced by a strain of streptomyces setonii. taxonomy, fermentation, isolation and characterization. | 16-deethylindanomycin (a83094a) is a novel pyrrole-ether antibiotic produced by a strain of streptomyces setonii. the antibiotic, which is structurally similar to indanomycin (x-14547a), is active in vitro against gram-positive bacteria as well as coccidia. | 1988 | 3182398 |
| purification and characterization of two forms of cyclic gmp-dependent protein kinase from bovine aorta. | cyclic gmp-dependent protein kinase in extracts of bovine aortic tissue eluted from deae-cellulose ion-exchange resins as two distinct peaks of activity. this elution pattern was preserved when the peaks were combined, precipitated with ammonium sulfate, dialyzed, and rechromatographed. proteolysis did not appear to account for the two forms of kinase because (i) aging of the extract did not cause interconversion of the two forms, and (ii) both forms retained cgmp sensitivity unlike the proteoly ... | 1988 | 3182865 |
| [griseochelin methyl ester, a new polyether derivative with antiviral activity]. | the methylester of griseochelin (1) is a new chemically-made antiviral derivate of the antibiotic griseochelin isolated from fermentations of streptomyces griseus. it belongs to the polyether group and possesses antiviral activity against enveloped rna and dna viruses cultivated in chicken embryo cells (cec), namely influenzavirus a/wsn, vesicularstomatitis virus (indiana), vaccinia virus (lister) and herpes simplex hominis virus type 1 (kupka). the methylester of griseochelin failed to show vir ... | 1988 | 3212020 |
| comparative molecular model building of two serine proteinases from cytotoxic t lymphocytes. | two genes that are expressed when precursor cytotoxic t lymphocytes are transformed to t killer cells have been cloned and sequenced. the derived amino acid sequences, coding for cytotoxic cell protease 1 (ccp1) and hannuka factor (hf) are highly homologous to members of the serine proteinase family. comparative molecular model building using the known three-dimensional structures and the derived amino acid sequences of the lymphocyte enzymes has provided useful structural information, especiall ... | 1988 | 3237717 |
| peptide digest studies of polymorphic proteins of plasmodium falciparum. | previous work has shown that when a large number of plasmodium falciparum isolates are examined by two dimensional electrophoresis, over 100 different proteins can be detected, 15 of which show polymorphism in electrophoretic characters. eight of these proteins have now been subjected to limited proteolysis. two methods of digestion were used: enzymic, with streptomyces griseus pronase e and chemical, using n-chlorosuccinimide to break proteins at tryptophan residues. when different forms of eac ... | 1987 | 3302701 |
| the microbial environment and intestinal nematode infections of heligmosomoides polygyrus in laboratory mice. | the difficulty of establishing primary infections of heligmosomoides polygyrus (= nematospiroides dubius) in ash/csi mice in the laboratory animal house at royal holloway and bedford new college during a recent autumn and spring period was associated with a syndrome of worm distortion, together with zero or low worm establishment and reduced fecundity (eggs/female worm). the eggs produced were non-viable and the egg capsule comprised a rumpled lipid and ruptured chitin layer. the egg size and pe ... | 1988 | 3392943 |
| binding and activation of c4 and c3 on the red cell surface by non-complement enzymes. | we investigated the binding of c4 and c3 to red cell surfaces by non-complement enzymes. cell bound c components were quantitated by a radioimmunoassay, the chain structure of bound components was analyzed by western blotting and the hemolytic activity of bound components was determined. trypsin, chymotrypsin, plasmin, elastase, thrombin, kallikrein and enzymes from bacillus subtilis, staphylococcus aureus and streptomyces griseus all were found capable of binding c4b and c3b to sheep red cells. ... | 1988 | 3412332 |
| method for the detection and quantitation of chitinase inhibitors in fermentation broths; isolation and insect life cycle effect of a82516. | a new method of screening for chitinase inhibitors in crude fermentation broths as a means of discovering new insecticidal leads has been developed. in this procedure soluble remazol brilliant violet 5r dye-coupled chitin degradation products released from insoluble chitin azure substrate by hydrolysis with streptomyces griseus chitinase are filtered in 0.45 micron millititer ha 96 well filtration plates and collected in 96 well microtiter plates. inhibitors of this reaction are detected by a de ... | 1987 | 3429340 |
| [formation of a-factor-type regulators by representatives of the genus micromonospora]. | it is known that substances similar in their chemical structure and biological action to a-factor, a regulator of streptomyces griseus differentiation are widely observed in streptomycetes. the present paper is concerned with studies on capacity for producing a-factor-like regulators in 13 strains belonging to 11 species of genus micromonospora. it was shown that 7 strains had an a-factor-deficient test mutant effect analogous to the effect of a-factor. relation of a-factor-like substance produc ... | 1987 | 3435093 |
| biotransformation of aklanonic acid by blocked mutants of anthracycline-producing strains of streptomyces galilaeus and streptomyces peucetius. | aklanonic acid is an anthracyclinone-related pigment that was found to be an intermediate at an early stage of the biosynthesis of daunomycinone glycosides by streptomyces griseus. we now isolated anthracycline-negative mutants of other streptomyces species and used them in feeding experiments with both natural and radioactive aklanonic acid. in cultures of the mutant streptomyces galilaeus s 727 exogenous aklanonic acid was biotransformed to cinerubin a as the major product. the spectrum of the ... | 1986 | 3464736 |
| crystal and molecular structures of the complex of alpha-chymotrypsin with its inhibitor turkey ovomucoid third domain at 1.8 a resolution. | the molecular structure of the complex between bovine pancreatic alpha-chymotrypsin (ec 3.4.4.5) and the third domain of the kazal-type ovomucoid from turkey (omtky3) has been determined crystallographically by the molecular replacement method. restrained-parameter least-squares refinement of the molecular model of the complex has led to a conventional agreement factor r of 0.168 for the 19,466 reflections in the 1.8 a (1 a = 0.1 nm) resolution shell [i greater than or equal to sigma (i)]. the r ... | 1987 | 3477645 |
| determination of the cleavage specificity of streptomyces griseus protease b in the presence of guanidinium chloride. | streptomyces griseus protease b (sgpb), a pronase enzyme, has been shown to be stable and active in the presence of 6.0 m guanidinium chloride (siegel, s. et al. (1972) j. biol. chem. 247, 4155-4159). in order to determine the cleavage specificity of this unusual enzyme under denaturing conditions, 12 peptides of known amino acid sequence were hydrolyzed by sgpb in the absence and presence of 6.0 m guanidinium chloride. the new n-terminal amino acids produced by the action of sgpb were dansylate ... | 1987 | 3479397 |
| a survey of the effects of proteases and glycosidases on culture of rabbit morulae to blastocysts. | the effects of a range of commercially available proteases and glycosidases on blastocyst development and hatching were examined on rabbit embryos cultured from the morula stage in a defined medium supplemented with charcoal-treated bovine serum albumin. the proteases tested were trypsin, alpha-chymotrypsin, thrombin, elastase, plasmin, papain, clostripain, collagenase, streptomyces griseus protease and cathepsin c. the glycosidases tested were neuraminidase, alpha-mannosidase, beta-galactosidas ... | 1986 | 3531506 |
| characterization of the receptor for cholera toxin and escherichia coli heat-labile toxin in rabbit intestinal brush borders. | 125i-labelled heat-labile toxin (from escherichia coli) and 125i-labelled cholera toxin bound to immobilized ganglioside gm1 and balb/c 3t3 cell membranes with identical specificities, i.e. each toxin inhibited binding of the other. binding of both toxins to balb/c 3t3 cell membranes was saturable, with 50% of maximal binding occurring at 0.3 nm for cholera toxin and 1.1 nm for heat-labile toxin, and the number of sites for each toxin was similar. the results suggest that both toxins recognize t ... | 1986 | 3541910 |
| separation of organic dust from microorganism suspensions by partitioning in aqueous polymer two-phase systems. | conidia of penicillium brevi-compactum and aspergillus fumigatus, sporangiospores of rhizopus rhizopodiformis, spores of streptomyces griseus, and bacterial cells of bacillus subtilis were partitioned in two-phase systems consisting of dextran, polyethylene glycol, substituted positively charged sulfonylpolyethylene glycol, and water. at a ph of 2.8 in the system, the microorganisms showed 60 to 90% affinity for the upper, polyethylene glycol-rich phase, except for cells of b. subtilis, which we ... | 1987 | 3579285 |
| the complete amino acid sequence of rice bran trypsin inhibitor. | the complete amino acid sequence of a double-headed trypsin inhibitor (rbti) from rice bran was determined by a combination of limited proteolysis of the native inhibitor with streptomyces griseus trypsin at ph 3 and conventional methods. rbti consists of 133 amino acid residues including 18 half-cystine residues which are involved in 9 disulfide bridges in the molecule. the limited proteolysis at ph 3 produced a major split of lys(83)-met(84) and a minor split of arg(107)-val(108) together with ... | 1987 | 3667571 |
| characterization of new viridomycins and requirements for production in cultures of streptomyces griseus. | cultures of streptomyces griseus grown under phosphate-limiting conditions produced a complex of green products. three of these were separated from the mixture and characterized. one was identified as viridomycin a, the ferrous chelate of 4-hydroxy-3-nitrosobenzaldehyde; the second (actinoviridin a) was the corresponding carboxylic acid chelate and the third (viridomycin e) was a hybrid chelate containing both the aldehyde and acid ligands. only two out of nine strains of s. griseus examined pro ... | 1987 | 3680025 |
| s-[2-(n7-guanyl)ethyl]glutathione, the major dna adduct formed from 1,2-dibromoethane. | the reaction of 1,2-dibromoethane and glutathione with dna in the presence of glutathione s-transferase results in the formation of a single major dna adduct, which can be released by thermal hydrolysis at neutral ph and separated by octadecylsilyl and propylamino high-performance liquid chromatography. the same dna adduct is the only major one formed in livers of rats treated with 1,2-dibromo[1,2-14c]ethane. the dna adduct was identified as s-[2-(n7-guanyl)ethyl]glutathione: (1) the chromatogra ... | 1986 | 3707941 |
| actinomycotic mycetoma in a cat. | actinomycotic mycetoma, a chronic, progressive infection of the subcutaneous tissue characterized by tumefaction, draining sinuses, and grains, was diagnosed in the right hindlimb of a young adult, male cat. the organisms that cause actinomycetoma are soil or plant saprophytes that gain entrance to the skin through abrasion or traumatic implantation. streptomyces griseus, an organism generally considered to be a saprophyte, was cultured bacteriologically. despite extensive surgery and long-term ... | 1986 | 3759615 |
| capuramycin, a new nucleoside antibiotic. taxonomy, fermentation, isolation and characterization. | a new antibiotic capuramycin was isolated from the culture filtrate of streptomyces griseus 446-s3 by adsorption and partition column chromatography. based on its chemical structure being an uracil nucleoside with a caprolactam substituent, this substance was named as capuramycin. this paper describes the taxonomy of a producing organism, fermentation, isolation, characterization and biological properties of capuramycin. | 1986 | 3759655 |
| ovomucoid third domains from 100 avian species: isolation, sequences, and hypervariability of enzyme-inhibitor contact residues. | ovomucoids were isolated from egg whites of 100 avian species and subjected to limited proteolysis. from each an intact, connecting peptide extended third domain was isolated and purified. these were entirely sequenced by single, continuous runs in a sequencer. of the 106 sequences we report (five polymorphisms and chicken from the preceding paper [kato, i., schrode, j., kohr, w. j., & laskowski, m., jr. (1986) biochemistry (preceding paper in this issue)]), 65 are unique. in all cases except os ... | 1987 | 3828298 |
| phospholipid protection against proteolysis of d-beta-hydroxybutyrate dehydrogenase, a lecithin-requiring enzyme. | d-beta-hydroxybutyrate dehydrogenase is a lipid-requiring enzyme which is localized on the inner face of the mitochondrial inner membrane. the apodehydrogenase, i.e. the purified enzyme devoid of lipid, has been purified from beef heart mitochondria and as such is inactive. it can be reactivated by insertion into phospholipid vesicles containing lecithin. proteolytic digestion with different proteases has been carried out to obtain insight into the orientation of the enzyme in the membrane and t ... | 1985 | 3881438 |
| electron density calculations as an extension of protein structure refinement. streptomyces griseus protease a at 1.5 a resolution. | ab initio quantum mechanical calculations have been used to obtain details of the electron density distribution in a high-resolution refined protein structure. it is shown that with accurate atomic co-ordinates, electron density may be calculated with a quality similar to that which can be obtained directly from crystallographic studies of small organic molecules, and that this density contains information relevant to the understanding of catalysis. atomic co-ordinates from the 1.8 a and 1.5 a r ... | 1985 | 3892015 |
| the 1.8 a structure of the complex between chymostatin and streptomyces griseus protease a. a model for serine protease catalytic tetrahedral intermediates. | the naturally occurring serine protease inhibitor, chymostatin, forms a hemiacetal adduct with the catalytic ser195 residue of streptomyces griseus protease a. restrained parameter least-squares refinement of this complex to 1.8 a resolution has produced an r index of 0 x 123 for the 11,755 observed reflections. the refined distance of the carbonyl carbon atom of the aldehyde to o gamma of ser195 is 1 x 62 a. both the r and s configurations of the hemiacetal occur in equal populations, with the ... | 1985 | 3892018 |
| refined structure of alpha-lytic protease at 1.7 a resolution. analysis of hydrogen bonding and solvent structure. | the structure of alpha-lytic protease, a serine protease produced by the bacterium lysobacter enzymogenes, has been refined at 1.7 a resolution. the conventional r-factor is 0.131 for the 14,996 reflections between 8 and 1.7 a resolution with i greater than or equal to 2 sigma (i). the model consists of 1391 protein atoms, two sulfate ions and 156 water molecules. the overall root-meansquare error is estimated to be about 0.14 a. the refined structure was compared with homologous enzymes alpha-c ... | 1985 | 3900416 |
| affinity gel titration: quantitative analysis of the binding equilibrium between immobilized protein and free ligand by a continuous titration procedure. | a new method, called affinity gel titration, for analyzing the specific interaction between an immobilized protein and a ligand molecule is presented. only one or two experimental runs permit the determination of not only the equilibrium constant but also the amount of immobilized protein. a suspension of the immobilized protein on agarose gel beads is confined in a constant volume mixing cell. a solution of a specific ligand molecule of constant concentration is introduced into the cell so that ... | 1985 | 3907409 |
| turkey ovomucoid third domain inhibits eight different serine proteinases of varied specificity on the same ...leu18-glu19 ... reactive site. | we show that eight different serine proteinases--bovine chymotrypsins a and b, porcine pancreatic elastase i, proteinase k, streptomyces griseus proteinases a and b, and subtilisins bpn' and carlsberg--interact with turkey ovomucoid third domain at the same leu18-glu19 peptide bond, the reactive site of the inhibitor. turkey ovomucoid third domain was converted to modified (the reactive site peptide bond hydrolyzed) form as documented by sequencing. complexes of all eight enzymes both with virgi ... | 1985 | 3907700 |
| 4-aminobutyrate:2-oxoglutarate aminotransferase of streptomyces griseus: purification and properties. | 4-aminobutyrate: 2-oxoglutarate aminotransferase of streptomyces griseus was purified to homogeneity on disc electrophoresis. the relative molecular mass of the enzyme was found to be 100 000 +/- 10 000 by a gel filtration method. the enzyme consists of two subunits identical in molecular mass (mr 50 000 +/- 1000). the transaminase is composed of 486 amino acids/subunit containing 10 and 12 residues of half-cystine and methionine respectively. the nh2-terminal amino acid sequence of the enzyme w ... | 1985 | 3917915 |
| biosynthesis of fredericamycin a, a new antitumor antibiotic. | fredericamycin a (fm a), produced by a strain of streptomyces griseus, represents a new structural class of antitumor antibiotics containing a spiro ring system. studies on the producer organism showed that glucose in the fermentation medium is not utilized until late in the growth stage, just prior to synthesis of fm a. [14c]glucose tracer experiments demonstrated that glucose is incorporated into fm a by catabolism to acetate. biosynthetic enrichment of fm a with single- and double-labeled [13 ... | 1985 | 3919762 |
| biotransformations of 1',2'-dihydrorotenone by streptomyces griseus. | dihydrorotenone yields three major products when incubated with growing cultures of streptomyces griseus. these were isolated by solvent extraction and characterized by spectral methods as 1',2'-dihydro-6abeta-hydroxyrotenone, 1',2'-dihydro-2',6abeta-dihydroxyrotenone, and 1',2'-dihydro-1',6abeta-dihydroxyrotenone. | 1985 | 3920966 |
| isolation of streptomycin-nonproducing mutants deficient in biosynthesis of the streptidine moiety or linkage between streptidine 6-phosphate and dihydrostreptose. | eight streptidine idiotrophic mutants (sd20, sd81, sd141, sd189, sd245, sd261, sd263, and sd274) which required streptidine to produce streptomycin were derived from streptomyces griseus atcc 10137 by uv mutagenesis. by both the characterization of intermediates accumulated by the idiotrophs and the assay of enzymes involved in streptidine biosynthesis, the biochemical lesions of the mutants were deduced as follows: sd20 and sd263, transamination; sd81, sd261, and sd274, phosphorylation; sd141, ... | 1985 | 3922295 |
| synergistic effect of cobalt on the induction by a-factor of the formation of aerial mycelium and anthracyclines by a blocked mutant of streptomyces griseus. | cobalt ions synergistically supported the inducing effect of a-factor 1a on the formation of aerial mycelium and anthracyclines by the blocked s. griseus mutant 86. this suggested an involvement of effector 1a in the transport or metabolism of this trace element. in accordance with the proposed role of 1a as an autoregulatory secondary metabolite, the parent strain s. griseus ja 3933 secreted 1a into the medium after a decrease of the specific growth rate and before the onset of antibiotic produ ... | 1985 | 3926984 |
| [spontaneous variation of streptomyces griseus lysing staphylococci]. | spontaneous variation of str. griseus was studied. 7 variants were isolated which go into the homologous series of the hereditary variability of apigment actinomycetes. the confidence intervals of the correlative relationship between the cultural and morphological features of the spontaneous variants and the level of their lytic activity did not over lap at p 0.95, which indicates that the difference is also significant by this feature. a possibility for a certain increase of the culture activit ... | 1985 | 3927834 |
| growth of bacteria on chitin, fungal cell walls and fungal biomass, and the effect of extracellular enzymes produced by these cultures on the antifungal activity of amphotericin b. | vibrio alginolyticus, streptomyces griseus, arthrobacter g12, bacillus sp. and cytophaga sp. were grown on solid and liquid media containing soluble and insoluble carbon sources. arthrobacter g12, bacillus sp. and cytophaga sp. grew well on media which contained fungal cell walls or fungal biomass as the main carbon source. all bacteria produced extracellular proteases and all bacteria except arthrobacter g12 produced extracellular chitinases. growth of cytophaga sp. on colloidal chitin was para ... | 1985 | 3929028 |
| evidence for the presence of an essential arginine residue in photoreactivating enzyme from streptomyces griseus. | butane-2,3-dione inhibits the enzymic activity of streptomyces griseus photoreactivating enzyme (pre). some characteristics of the inhibition, notably the enhancement by borate buffer and the reversibility, indicate that arginine residues are modified. from the kinetics of inhibition it can be concluded that a single essential arginine residue is involved. u.v.-irradiated dna, the substrate for pre, protects the enzyme against inactivation by butane-2,3-dione. this suggests that the essential ar ... | 1985 | 3929771 |
| preparation of labeled aklanonic acid and its bioconversion to anthracyclinones by mutants of streptomyces griseus. | 1985 | 3930456 | |
| characterization and regulation of p-aminobenzoic acid synthase from streptomyces griseus. | p-aminobenzoic acid synthase (paba synthase) of streptomyces griseus catalyses the conversion of chorismic acid to p-aminobenzoic acid (paba), a precursor of the aromatic p-aminoacetophenone moiety of candicidin, a polyene macrolide antibiotic. this enzyme uses glutamine or ammonia as amino donors for paba formation. enzyme extracts converted [14c]chorismic acid to labelled paba. paba synthase was present in s. griseus imru 3570 only during the antibiotic producing phase. no detectable levels of ... | 1985 | 3930655 |
| possible role of streptomycin released from spore cell wall of streptomyces griseus. | vegetative mycelia and spores of the investigated high- and low-producer strains of streptomyces griseus bound significant amounts (4%) of streptomycin, which could be removed by increasing ionic strength. the release of antibiotic from the spores was easier when the spores were germinating. this phenomenon is considered to play an ecological role. we suppose that the streptomycin released during the germination process may protect the young hyphae from the different bacteria growing in the micr ... | 1985 | 3931550 |
| purification and characterization of endo-beta-n-acetylglucosaminidase of aspergillus oryzae. | an endo-beta-n-acetylglucosaminidase which hydrolyzes the n,n'-diacetylchitobiosyl linkage in asparagine-linked oligosaccharides was purified from the enzyme product of aspergillus oryzae. its substrate specificity was similar to that of endo-beta-n-acetylglucosaminidase h from streptomyces griseus with respect to the relative activities toward the glycopeptides obtained from ovalbumin and bovine igg. the present endoglycosidase exhibited a broad optimum ph range and was relatively stable. metal ... | 1985 | 3934149 |
| high-performance affinity chromatography of trypsins on asahipak gs-gel coupled with p-aminobenzamidine. | an adsorbent for high-performance affinity chromatography of trypsins was prepared, based on a micro-particulate polyvinyl alcohol gel for high-performance liquid chromatography, asahipak gs-gel. after the hydroxyl groups had been activated with 1,1'-carbonyldiimidazole, 6-aminohexanoic acid was coupled as a spacer, then p-aminobenzamidine, a specific ligand for trypsin-family enzymes, was immobilized on the spacer. fluorometric detection of eluted protein and on-line assay of enzyme activity us ... | 1985 | 3934203 |
| interactions of derivatives of guanidinophenylalanine and guanidinophenylglycine with streptomyces griseus trypsin. | the rates of hydrolysis of the ester, amide and anilide substrates of p-guanidino-l-phenylalanine (gpa) by streptomyces griseus trypsin (s. griseus trypsin) were compared with those of arginine (arg) substrates. the specificity constant (kcat/km) for the hydrolysis of gpa substrates by the enzyme was 2-3-times lower than that for arginine substrates. the kcat and km values for the hydrolysis of n alpha-benzoyl-p-guanidino-l-phenylalanine ethyl ester (bz-gpa-oet) by s. griseus trypsin are in the ... | 1985 | 3935172 |
| identification of transformation products arising from bacterial oxidation of codeine by streptomyces griseus. | 14-hydroxycodeine and norcodeine were rigorously identified as products arising from codeine oxidation by streptomyces griseus atcc 10137. both products were routinely detected in extracted culture filtrates after growth of cells in the presence of codeine for 1 week. under these conditions, about 4 mol% of the codeine starting material was consumed, with norcodeine and 14-hydroxycodeine representing the only identifiable transformation products (molar ratio, 4:1, respectively). extraction of a ... | 1985 | 3936418 |
| interactions of streptomyces subtilisin inhibitor with streptomyces griseus proteases a and b. enzyme kinetic and computer simulation studies. | streptomyces subtilisin inhibitor (ssi), a dimeric protein that strongly inhibits subtilisins, was shown to form tight inhibitory complexes with streptomyces griseus proteases a and b (sgpa and sgpb). the apparent dissociation constants of the sgpa-ssi and sgpb-ssi complexes were found to be orders of magnitude less than those of subtilisin-ssi complexes. using the known atomic coordinates for sgpa and ssi, the highly complementary nature of the surface geometries of the two proteins was confirm ... | 1985 | 3936849 |
| [leukaemomycin-blocked mutants of streptomyces griseus and their pigments. iii. 11-desoxydaunomycinone derivatives from the mutant zimet 43699/g44]. | the isolation and identification of several anthracyclinones (designated as g44-k4/5, g44-g1, g44-g2) and anthracyclines (g44-a, b, c, d, e, f, g) produced by the mutant strain imet ja 3933/g44 of the daunomycin-producing streptomyces griseus strain imet ja 3933 are described. g44-k4/5 was found to be identical with 7,11-dideoxy-13-dihydrodaunomycinone previously isolated from a mutant strain of streptomyces coeruleorubidus. compound g44-g1 was identified as 11-deoxydaunomycinone, the aglycone o ... | 1985 | 3937895 |
| [production of vitamin b12 by fermentation]. | in this work, the methods and technology of vitamin b12 production were studied on laboratory scale. the microorganisms used for experiments were streptomyces olivaceus ifo 3409, streptomyces olivaceus cbs 355.53 and streptomyces griseus cbs 161.45 which were brought from foreign countries. vitamin b12 activity that have been obtained from fermentation experiments of each microorganisms are determined by microbiological assays using lactobacillus leichmannii ifo 3073 (atcc 4797) which is a test ... | 1985 | 3938519 |
| isolation of two trypsin inhibitors from resting seeds of the white bush (cucurbita pepo var. patissonina) and their properties. | two trypsin inhibitors, cppti-i and cppti-ii of mr 3 250 and 7 850, respectively, were isolated from resting white bush seeds. both inhibitors are cysteine-rich proteins. in addition to trypsin, they inhibit a trypsin-like enzyme isolated from streptomyces griseus proteinase but they do not act on chymotrypsin, kallikrein or subtilopeptidase a. the isolated inhibitors contain a lysine residue in position p1 of the reactive site. | 1985 | 3938588 |
| the crystal and molecular structure of the third domain of silver pheasant ovomucoid (omsvp3). | omsvp3 and omtky3 (third domains of silver pheasant and turkey ovomucoid inhibitor) are kazal-type serine proteinase inhibitors. they have been isomorphously crystallized in the monoclinic space group c2 with cell dimensions of a = 4.429 nm, b = 2.115 nm, c = 4.405 nm, beta = 107 degrees. the asymmetric unit contains one molecule corresponding to an extremely low volume per unit molecular mass of 0.0017 nm3/da. data collection was only possible for the omsvp3 crystals. orientation and position o ... | 1985 | 3971987 |
| the generation of additional antibiotic resistance by protoplast regeneration of a streptomyces griseus strain. | 1985 | 3972723 | |
| new antibiotic-producing streptomycetes, selected by antibiotic resistance as a marker. i. new antibiotic production generated by protoplast fusion treatment between streptomyces griseus and s. tenjimariensis. | a novel antibiotic was found after performing an interspecific fusion treatment between streptomyces griseus and s. tenjimariensis by the selection of clones with a unique antibiotic resistance. nonantibiotic-producing mutants of streptomycin (sm)-producing s. griseus ss-1198 with resistance to sm and istamycin (is)-producing s. tenjimariensis ss-939 with resistance to kanamycin (km) were protoplasted, mixed with polyethyleneglycol and regenerated. resistant clones to both sm and km were found a ... | 1985 | 3972729 |
| new antibiotic-producing streptomycetes, selected by antibiotic resistance as a marker. ii. features of a new antibiotic-producing clone obtained after fusion treatment. | a new antibiotic-producing streptomyces strain sk2-52 obtained by a protoplast fusion treatment between streptomyces griseus np1-1 and s. tenjimariensis nm16 showed taxonomical features identical with those of s. griseus. the strain resistant to wider range of aminoglycoside antibiotics than the parental strains. this multiple resistance corresponded to the activities of streptomycin kinase and acetyltransferase which were probably derived from s. griseus np1-1. clones with fast-growth and reduc ... | 1985 | 3972730 |
| streptomyces beta-alanine:alpha-ketoglutarate aminotransferase, a novel omega-amino acid transaminase. purification, crystallization, and enzymologic properties. | an enzyme which catalyzes the transamination of beta-alanine with alpha-ketoglutarate was purified to homogeneity from streptomyces griseus ifo 3102 and crystallized. molecular weight of the enzyme was found to be 185,000 +/- 10,000 by a gel-filtration method. the enzyme consists of four subunits identical in molecular weight (51,000 +/- 1,000). the transaminase is composed of 483 amino acids/subunit containing 7 and 8 residues of half-cystine and methionine, respectively. the enzyme exhibits ab ... | 1985 | 3972825 |
| l-phenylalanine and l-tyrosine catabolism by selected streptomyces species. | l-phenylalanine and l-tyrosine were completely catabolized through homogentisate by streptomyces setonii 75vi2 but only partially degraded by streptomyces badius 252, streptomyces sioyaensis p5, streptomyces viridosporus t7a, and streptomyces sp. strain v7. intermediates of catabolism were confirmed by thin-layer, gas, and high-pressure liquid chromatography. homogentisate 1,2-dioxygenase was present in all cell extracts. | 1985 | 3994376 |
| the cloned streptomyces bikiniensis a-factor determinant. | by cleavage with restriction endonucleases and cloning the resultant fragments, the a-factor determinant cloned from streptomycin-producing streptomyces bikiniensis (horinouchi et al., j. bacteriol. 158: 481 approximately 487, 1984) was narrowed down to a 1.1-kilobase (kb) fragment. a hybrid multicopy plasmid (pafb15) carrying the 1.1-kb fragment conferred a-factor production in a large quantity to a-factor-deficient mutants of s. bikiniensis and streptomyces griseus as well as afsa mutants of s ... | 1985 | 4019312 |
| heterologous hybridization of bacterial dna to the endoglucanases a and b structural genes cela and celb of clostridium thermocellum. | dna from various cellulolytic and non-cellulolytic bacteria was found to hybridize to clostridium thermocellum ncib10682 dna fragments carrying the structural genes cela and celb which code for endoglucanases a and b. homology to cela was detected in agrobacterium rhizogenes, azospirillum brasilense, bacillus subtilis, cellulomonas sp., clostridium stercorarium, erwinia chrysanthemi, pseudomonas solanacearum and streptomyces griseus. homology to celb was detected only in b. subtilis, c. stercora ... | 1985 | 4083831 |
| proteins of vesicular stomatitis virus and of phenotypically mixed vesicular stomatitis virus-simian virus 5 virions. | the identity of the glycoprotein of vesicular stomatitis virus (vsv) as the spike protein has been confirmed by the removal of the spikes with a protease from streptomyces griseus, leaving bullet-shaped particles bounded by a smooth membrane. this treatment removes the glycoprotein but does not affect the other virion proteins, apparently because they are protected from the enzyme by the lipids in the viral membrane. the proteins of phenotypically mixed, bullet-shaped virions produced by cells m ... | 1971 | 4108932 |
| elastolytic and chymotryptic activity in streptomyces griseus enzyme. | 1973 | 4127373 | |
| effect of a-factor on the growth of asporogenous mutants of streptomyces griseus, not producing this factor. | 1973 | 4131140 | |
| properties of ribosomes from streptomyces erythreus and streptomyces griseus. | ribosomes from an erythromycin-producing strain, streptomyces erythreus, lacked affinity for erythromycin and were also resistant to other macrolide antibiotics (leucomycin, spiramycin, and tylosin) and to lincomycin, whereas streptomyces griseus b(3) ribosomes were susceptible to all of these antibiotics. | 1974 | 4138441 |
| nadph-dependent formation of thymidine diphosphodihydrostreptose from thymidine diphospho-d-glucose in a cell-free system from streptomyces griseus and its correlation with streptomycin biosynthesis. | 1974 | 4151722 | |
| endo-beta-n-acetylglucosaminidases acting on the carbohydrate moieties of glycoproteins. the differential specificities of the enzymes from streptomyces griseus and diplococcus pneumoniae. | 1974 | 4154323 | |
| [hydrolysis of the collagen from calf hide by a crystalline protease of streptomyces griseus]. | 1973 | 4196875 | |
| [chromatographic study of the cationic and anionic proteases of streptomyces griseus]. | 1973 | 4196876 | |
| [separation of the crystalline complex of streptomyces griseus proteases and a study of its enzymatic composition]. | 1972 | 4197055 | |
| the amino acid sequence of streptomyces griseus trypsin. i. the peptic peptides. | 1973 | 4199084 | |
| the proteolytic enzymes of the k-1 strain of streptomyces griseus obtained from a commercial preparation (pronase). purification and characterization of the aminopeptidases. | 1973 | 4199257 | |
| production of candicidin by streptomyces griseus. | 1973 | 4199289 | |
| [metabolic products of microorganisms. 122. relations between macrotetrolid production and k+ transport of streptomyces griseus (author's transl)]. | 1973 | 4204486 | |
| purification and properties of an endo-beta-n-acetylglucosaminidase from streptomyces griseus. | 1974 | 4204552 | |
| alteration of rna synthesis in vitro with an endogenous regulating factor of cytodifferentiation of streptomyces griseus. | 1973 | 4205012 | |
| induced production of beta-galactosidase in streptomyces griseus. | 1973 | 4205013 | |
| studies of the heterogeneity of streptomyces griseus protease. isolation and characterization of an alkaline serine protease from commercial pronase-p derived from streptomyces griseus k1. | 1973 | 4205061 | |
| studies on the catalytic mechanism of a serine protease from streptomyces griseus. | 1974 | 4205936 | |
| [obtaining homogeneous carboxypeptidase from streptomyces griseus]. | 1974 | 4207328 | |
| m-81, a new peptide antibiotic produced by streptomyces griseus subsp. psychrophilus at moderate temperature. | 1974 | 4207785 | |
| the 4.5 angstrom resolution structure of a bacterial serine protease from streptomyces griseus. | 1974 | 4208242 | |
| [new (technical) protease preparation of streptomyces griseus]. | 1973 | 4208391 | |
| the amino acid sequence of streptomyces griseus trypsin. ii; the tryptic peptides. | 1974 | 4209619 | |
| isolation and characterization of ribosomes from streptomyces griseus spores. | 1973 | 4210345 | |
| effect of boric acid on the catalytic activity of streptomyces griseus protease 3. | 1974 | 4211952 | |
| effect of ph on the catalytic activity of streptomyces griseus protease 3. | 1974 | 4211962 | |
| inhibition of streptomyces griseus protease b by peptide chloromethyl ketones: partial mapping of the binding site and identification of the reactive residue. | 1974 | 4212092 | |
| the amino acid sequence and predicted structure of streptomyces griseus protease a. | 1974 | 4214713 | |
| action of streptomycin on the growth of streptomyces griseus. | 1974 | 4215551 | |
| zinc enzymes in commercial pronase-p. further characterization of the heterogeneity of the proteolytic enzymes of the k-1 strain of streptomyces griseus. | 1974 | 4216219 | |
| amino acid sequence of streptomyces griseus protease b, a major component of pronase. | 1974 | 4218101 | |
| [production of protease and streptomycin from the culture liquid of streptomyces griseus by methanol separation]. | 1973 | 4219049 |