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hyaluronic acid, b-glucuronidase, vitamin c and the immune defect in leprosy. 1976773848
has mycobacterium leprae been cultured in a cell-free artificial medium? 1976773849
a histopathologic study of striated muscle biopsies in leprosy.histopathologic changes in striated muscle biopsies in 50 cases of leprosy were studied; 40 being the lepromatous type and 10 the nonlepromatous type. all the biopsies were obtained from midportions of normal looking biceps muscles and paraffin embedded. sections cut in transverse and longitudinal planes were stained by hematoxylin and eosin, masson's trichrome, mallory's ptah, gomori's silver impregnation, and ziehl-neelsen's technic. lepromas, focal or confluent, in the endomysium, perimysium, ...1975776842
[sensitivity to dapsone, sulfamethoxypyridazine and ethionamide of mycobacterium leprae taken from patients treated by the drugs].suspensions of m. leprae from skin biopsies of patients treated with dapsone (dds) (four cases), sulfamethoxypyridazine (smp) (six cases), and ethionamide (eth) (seven cases), were inoculated into mouse foot pads and their sensitivity for the different drugs determined. two strains were dds resistant. resistance appeared after 13 and 14 years respectively after the start of treatment. five strains were isolated from patients treated with smp. relapses during sulfonamide treatment are considered ...1975776843
the role of arthropods in the transmission of leprosy. 1976776845
the effect of dihydrofolate reductase inhibitors on mycobacterium leprae in the mouse foot pad. 1976776847
dds resistance in ethiopia - a progress report. 1976776848
the incidence of dds resistance in lepromatous patients in costa rica: their metabolic disposition of dds. 1976776849
drug resistant leprosy--a comparison between proven dapsone and proven thiambutosine resistance. 1976776850
mycobacterium leprae persisters after treatment with dapsone and rifampicin. 1976776851
long-term treatment of dapsone-resistant leprosy with rifampicin: clinical and bacteriological studies. 1976776852
treatment failure in leprosy. 1976776854
the bactericidal effect of rifampicin on m. leprae in man: a) single doses of 600, 900 and 1200 mg; and b) daily doses of 300 mg. 1976776856
preliminary studies with the lymph node lymphocytes of m. leprae infected mice. 1976776857
the presence of systemic and local activated macrophages in mice infected in the foot pad with mycobacterium leprae and m. marinum.a highly sensitive assay for quantitating the presence of activated macrophages was employed to show that foot pad infection of mice with neither m. leprae nor m. marinum induced a population of activated peritoneal macrophages. in vitro attempts to demonstrate local activated macrophages in the foot pads of m. leprae infected mice failed, but, because of the technical problems encountered, do not preclude their presence. additional studies are being carried out.1976776858
taxonomic studies on the leprosy bacillus. 1976776859
vaccination of mice against m. leprae infection. 1976776860
production of anti-m. leprae antibodies in man and mice under the impairment of cell mediated immunity. 1976776861
evaluation of cell mediated immunity in the histopathologic spectrum of leprosy using lymphocyte transformation test.lymphocyte transformation in response to pha, m. leprae and bcg with the use of measurements of 3h-thymidine uptake, was studied in 10 normal controls, and 71 leprosy patients of different types histologically classified according to the ridley-jopling scale. lepromin and ppd tests were done in each one of them. it was found that there was a generalized depression of immune responsiveness to pha in all forms of leprosy. the immune responsiveness of lymphocytes to m. leprae continuously and gradu ...1976776862
cell mediated and humoral immunity in "reversal reactions". 1976776863
attempts at growth of m. leprae in mice and observations on a case of probable contamination with another acid-fast bacillus. 1976776864
the who program for research on immunology of leprosy (immlep). 1976776865
attempts at cultivation of mycobacterium leprae in cell culture. 1976776866
a31 cell as a host of mycobacterium lepraemurium and mycobacterium leprae. 1976776867
progress in the radiolabeling of mycobacterium leprae. 1976776869
in vitro cultivation of leprosy bacilli. 1976776870
multiplication of mycobacterium leprae from large inocula in neonatally thymectomized lewis rats. 1976776872
preparation of protein from mycobacterium leprae, skin test responses and lymphoblast transformation in vaccinated armadillos. 1976776873
delayed hypersensitivity tests with mycobacterium leprae protein in leprous and non-leprous persons. 1976776874
cell walls of mycobacterium leprae.walls were prepared from m. leprae isolated from livers and spleens of infected armadillos. these contained mycolic acids and arabinogalactan, but the composition of the peptidoglycan differed from that of the other mycobacteria that have been studied, since it contained substantial amounts of glycine. the relationship of m. leprae to the other mycobacteria remains obscure.1976776875
airborne infection with mycobacterium leprae in mice. 1976776876
spaced clofazimine therapy of lepromatous leprosy.patients with previously untreated borderline-lepromatous or fully lepromatous leprosy were treated with one of five clofazimine (b663) regimens: 1) 200 mg daily 6 days per week; 2) 100 mg three times weekly; 3) 300 mg weekly; 4) 600 mg every other week; and 5) 600 mg on 2 consecutive days every 4 weeks. after 24 weeks of treatment, the patients were randomly allocated to treatment either with 200 mg b663 daily 6 days per week (regimen 6) or with dapsone, beginning with a small dosage and increa ...1976779502
studies of the mouse foot pad technique for cultivation of mycobacterium leprae. 3. doubling time during logarithmic multiplication. 1976781441
acid-fast bacilli in the fingers of long-treated lepromatous patients. 1976781442
does droplet infection play a role in the transmission of leprosy? 1976781443
mycobacterium leprae in human skeletal muscle. 1975782385
letter: identification of mycobacterium ieprae. 1976782400
[cyto-morphological study of leprosy bacilli in the host cell (author's transl)]. 1975785134
[cyto-morphological study on m. leprae inoculated and grown in the mouse foot-pads (author's transl)]. 1975785135
growth of mycobacterium leprae and m. marinum in congenitally athymic (nude) mice. 1976785274
the histopathologic diagnosis of leprosy.the objective of this paper is to encourage histopathologists to recognize and diagnose leprosy so that it can be treated effectively at the earliest stage possible, before irreversible deformities result. recognition of the earliest form of leprosy-indeterminate-is emphasized. histopathologic descriptions are made and illustrated for the principal types of leprosy. emphasis is placed on (1) the need for dependable acid-fast staining of leprosy bacillus, (2) the non-specific infiltrate in indete ...1976785613
editorial: histochemical analysis of leprous tissue leads to cultivation of leprosy bacilli. 1976786212
infection of armadillos with mycobacterium leprae. ultrastructural studies of peripheral nerve.peripheral nerves of armadillos were studied 16 to 30 months after intradermal or intravenous inoculation with mycobacterium leprae. numerous bacilli were found within macrophages, schwann cells, and perineurial cells; endothelial cells, pericytes and fibroblasts were involved as well. the bacilli were characteristically contained in membrane-limited vacuoles that were interpreted as being phagosomes. some of the phagosomes contained granular, membranous, and vesicular debris considered to be ba ...1976786213
hepatic granulomas in leprosy. their relation to bacteremia.a clinicopathologic study of liver disease was conducted on 28 patients with leprosy who lived in taiwan. none of the patients exhibited symptoms or signs of liver disease. hepatic granulomas were found in 21 patients. histologically, the infiltrates were epithelioid, foam cell, and histiocytic in type. hepatic dysfunction was absent, except for mild sulfobromophthalein elevations in the severely infected cases. hepatic granulomas correlated with the cutaneous reactions in lepromatous leprosy, b ...1976786214
neonatally thymectomized lewis rats infected with mycobacterium leprae: response to primary infection, secondary challenge, and large inocula.several experiments were carried out to measure the ability of neonatally thymectomized lewis rats (ntlr) to limit multiplication of mycobacterium leprae. ntlr inoculated in one hind footpad with 10(7) viable m. leprae and challenged in the other hind footpad with 5 x 10(3) organisms simultaneously or 120 or 180 days later permitted multiplication in both sites. by contrast, immunologically intact rats similarly inoculated did not permit multiplication from either inoculum. ntlr and immunologica ...1976786892
leprosy-like disease occurring naturally in armadillos. editorial. 1976787707
qualitative histology and quantitative bacteriology in various tissues of 50 leprosy patients. 1976787709
[culture of leprosy-bacteria in laboratory]. 1976787746
separation of m. leprae from human leproma and the development of a cytoplasmic skin test antigen from purified bacilli.a successful method for purification of m. leprae from human leproma without subjection to heat has been developed. the "floater" phenomenon has been described which consists of bacillary tendency to float in the supernate when bacilli which are not autoclaved are separated from tissues by enzymatic digestion. a method for preparing cytoplasmic fractions from purified m. leprae has been developed for the production of a skin test antigen for leprosy. the cytoplasmic fraction of m. leprae elicit ...1976789262
macrophage function in leprosy.the macrophage function in patients with leprosy was assessed by estimating histochemically the acid phosphatase activity in skin biopsies and by assessment of phagocytic and lytic capability of in vitro cultured macrophages derived from peripheral blood monocytes, challenged with live m. leprae. acid phosphatase was demonstrated in skin biopsies of different groups of leprosy patients classified according to the ridley and jopling scale. the degree of acid phosphatase positivity was correlated ...1976789263
"leprosy" in wild armadillos. 1976789264
in vitro grown mycobacterium leprae probably a member of the mycobacterium scrofulaceum species. 1976789265
lymphocyte transformation test in leprosy; correlation of the response with inflammation of lesions.lymphocyte transformation tests (ltt) using 'whole washed" and 'sonicated" preparations of mycobacterium leprae (m. leprae) as antigen were studied in eighty-one patients with borderline leprosy. the results were correlated with the histological and the clinical pictures. there was a good correlation with the histological spectrum, ltt responses generally being higher in the borderline tuberculoid leprosy patients and lower in the borderline lepromatous. however, considerable variation was noted ...1976791549
early leprosy of the nose and throat. 1976792374
attempts at cultivation of mycobacterium leprae in cell cultures. 1976792535
the activity of a thiadiazole on mycobacterium leprae. 1976792890
loss of infectivity of mycobacterial and protozoal exoplasmic parasites after exposure in vitro to the polyenzymic cocktail 'pigo'. 1976793902
dapsone chemotherapy of mycobacterium leprae infection of the neonatally thymectomized lewis rat.in order to learn whether the neonatally thymectomized lewis rat (ntlr) infected with mycobacterium leprae could serve as a model for chemotherapeutic studies in a situation resembling that found in human lepromatous leprosy, ntlr inoculated with m. leprae either locally or intravenously 9 to 16 months earlier were treated for from 1.5 to 8.5 months with dapsone (4,4'-diaminodiphenylsulfone, dds) incorporated in the rat chow in the concentration providing the minimal inhibitory concentration of ...1976795312
electrophoretic patterns of serum proteins and immunoglobulin levels in mycobacterial infections--studies in mice infected with mycobacterium leprae and mycobacterium lepraemurium. 1976796470
autoradiographic studies of mycobacterium leprae. 1976796603
studies of the mouse foot-pad technique for cultivation of mycobacterium leprae. 4. statistical analysis of harvest data. 1976796604
report of the second immlep task force meeting 1-5 december, 1975. 1976796605
immunology and animal experimentation in leprosy. 1976797549
the effect of dioxyphenylalanine (dopa), amides and some potential sources of energy on the multiplication of mycobacterium leprae.the multiplication of 2 out of 3 m. leprae strains on a medium containing substances from digested non-acid-fast microorganisms or even free of them was significally promoted to d-3,4-dihydroxyphenylalanine (dopa). the following organic substances exerted growth-promoting effects on several strains: 0.02-0.10% concentrations of succinate greater than fumarate greater than alpha-ketoglutarate and acetate greater than glycerol; 0.2% concentrations of citrate and pyruvate greater than isonicotinami ...1976798593
[experimental chemotherapy of leprosy]. 1976798637
activity of ascorbic acid in inhibiting the multiplication of m. leprae in the mouse foot pad.ascorbic acid was fed to mice in concentrations of 0.05%, 0.15%, and 0.45% w/w in the diet. six months after inoculation of m. leprae into the foot pads, there were significantly fewer acid-fast bacilli harvested from animals receiving 0.15% and 0.45% w/w ascorbic acid than from control mice. on the other hand, m. leprae did multiply in mice fed ascorbic acid while no multiplication at all was observed in animals fed dapsone, clofazimine or rifampin. no toxic effects of ascorbic acid were noted ...1976798727
a simplified hyaluronic acid based culture medium for mycobacteria isolated from human lepromata.acid-fast bacilli multiplied in liquid culture media containing hyaluronic acid when inoculated with mycobacteria from a lepromatous leprosy nodule. the culture was readily subcultured at ten day intervals in the homologue media, but failed to grow in the dubos, middlebrook and lowenstein media. these findings confirm the results of skinsnes et al (1975). identification of this culture is not yet available, however it gives positive immunofluorescence with authentic anti-m. leprae serum. the obt ...1976798728
the lepromatous macrophage defect as related to vaccine development in leprosy. 1976798730
cultivation of myco. leprae--a breakthrough reported. 1976799201
experimental transmission of leprosy to animals: a preliminary note on attempt to transmit leprosy to the slender loris, loris tardigradus (linnaeus).a brief account of the slender loris, loris tardigradus (linnaeus) and some of its characteristics which render it suitable for leprosy transmission experiments are recorded. handling and inoculation methods are given with some of the preliminary inoculation site biopsy results.1976799202
bacteremia in leprosy and its relation to distribution of m. leprae in skin.evidence of bacillaemia through examination of heparinised blood smears was obtained in 17 of the 20 cases (85%) of untreated leprosy cases belonging to the spectrum ranging from bt to ll. among 17 cases whose blood smears were positive for afb, the endothelial cells of blood vessels in skin lesions showed afb in 11 instances (64.7%) and in 7 (41.2%) of these cases biopsies obtained from apparently normal skin also showed bacilli in the blood vessels. the fact that blood smears may show afb even ...1976799203
the lepromin test. 1976799204
acid-fast bacillary positivity in asymptomatic individuals in leprosy endemic villages around jhalda in west bengal.three general surveys of a village population of 7000 in a highly endemic area in purulia district, west bengal, have included clinical examination and earlobe skin snip examinations. multiple between-survey follow-up examinations have also been conducted of both bacillary positives and cases. in the general population 5.8% of the individuals showed bacteriological positivity with a concentration technique for afb without showing clinical signs of infection. during two years of observation clini ...1976799205
a modified method of harvesting m. leprae from foot-pads of mice.a modified technique of harvesting m. leprae from the foot-pads of mice is described. the method is simple and takes less time for its performance than the conventional techniques. the yield of bacilli is also better. no difficulties have been encountered in its application in these laboratories.1976799206
lepromatous leprosy--final discharge from treatment unit. 1976799208
early serodiagnosis of leprosy by indirect immunofluorescence.a method of fluorescent leprosy antibody absorption (fla-abs) test was described. this test was so sensitive that 81.8% of tuberculoid leprosy cases gave positive reaction even in early stage of the disease. more than 50% of indeterminate and contact cases were also positive in this test. the positive percentages as well as antibody-titers were increasing with clinical spectrum of leprosy, from tt to ll, being the highest in the latter. using absorbed and diluted serum from known lepromatous pat ...1976799209
correlation of morphology with viability of mycobacterium leprae.a concept has been developed in the recent years that the evenly stained 'solid' bacilli are living and the 'non-solid' forms are degenerate and dead. this communication presents the findings in experimental mice inoculated with material containing 1 to 10% solid evenly stained m. leprae and also with material containing 0% solid organisms. there was multiplication of the bacilli in both the groups. quantitatively, the yield also was not significantly different. these fundings do not support the ...1976799212
bacillaemia in reactive states of leprosy.35 cases of lepromatous and near-lepromatous cases of laprosy in reaction have been investigated for the presence of acid-fast bacilli in blood at the height of the reaction as well as at its subsidence. only 3 cases exhibited bacillaemia during reaction. it is therefore unlikely that dissemination of the disease is accentuated during reaction as commonly believed. further, the immune complexes demonstrated to be circulating during reaction are possibly formed by bacillary products and not by wh ...1976799213
significance of nine banded armandillo in biomedical leprosy research. 1976799214
[diagnosis of leprosy]. 1976799265
[drug sensitivity test of m. leprae using liquid media (author's transl)]. 1976799737
importance of immune reactions in leprosy. 1976799966
studies of experimental chemotherapy of leprosy. 1976800233
clofazimine in the treatment of dapsone resistant leprosy.dapsone resistant patients treated with clofazimine 100 mg daily show a consistent response to treatment. concern is expressed regarding the prolonged persistence of viable organisms during treatment, with the attendant risk of further resistance developing. the importance of prevention of this problem is discussed.1976800234
cultivation of mycobacterium leprae using semi-synthetic medium. 1976801024
cultivation of mycobacterium leprae and mycobacterium lepraemurium in cell culture. 1976801026
[attempts at growth of m. leprae in mice (author's transl)]. 1976801283
[cell-biological study on the acid-fast organisms isolated and cultivated from leprosy patients. 2. pathogenic behavior of the organism to experimental animals, and results of inoculation to mice foot-pads (author's transl)]. 1976801365
viability of mycobacterium leprae pretreated with rifampicin. 1976801367
lepromatoid lesion developed in nude mouse inoculated with mycobacterium leprae--animal transmission of leprosy. 1976801368
[the etiological mystery of sarcoidosis]. 1975804178
susceptibility of thymectomized and irradiated mice to challenge with several organisms and the effect of dapsone on infection with mycobacterium leprae.b6c3f1 mice that had been thymectomized at 8 to 12 weeks of age, subjected to 950 r of whole-body x irradiation, and transfused with syngeneic bone marrow were challenged in a footpad with mycobacterium leprae or m. marinum, or intravenously or intraperitioneally with listeria monocytogenes. also, mice inoculated with m. leprae in a hind footpad were administered dapsone in the mouse chow. the thymectomized-irradiated (t + r) mice did not survive as well as non-thymectomized mice when housed in ...1975804443
amyloid-related serum component (protein asc) in leprosy patients.the presence of amyloid-related serum component, protein asc, in serum samples from 63 leprosy patients was investigated. protein asc was detected in 38% of the patients. a correlation to the disease spectrum of leprosy was apparent: polar lepromatous cases, 64% positive; borderline lepromatous, 50%; borderline tuberculoid, 36%; subpolar tuberculoid, 17%; and polar tuberculoid, negative. antibody activity against the a antigen of mycobacterium leprae was also determined, showing a similar correl ...1975804451
antigenic evaluation of mycobacterium lepraemurium.immunodiffusion analysis of mycobacterium lepraemurium indicated the presence of at lease six antigens. comparative analysis of the m. lepraemurium antigen-antibody system with similar systems established for other mycobacterial species, showed that m. lepraemurium shared up to two antigens with other species. although our observations are in accord with some of the studies on the antigenic mosaic of m. lepraemurium, they are in disagreement with the observations of stanford (1973) concerning a ...1975805240
o-diphenoloxidase of mycobacterium leprae separated from infecected armadillo tissues.we reported earlier the occurrence of a unique o-diphenoloxidase in mycobacterium leprae recovered from lepromatous human tissues. no other source of m. leprae fro biochemical studies was available at the time. in the present report, properties of phenoloxidase in m. leprae separated from infected armadillo tissues are presented. the results show that the o-diphenoloxidase remains unaltered in the passage of the bacilli from the human to the the animal host, indicating that the enzyme is an intr ...1975807522
[in-vitro study of the effects of 4,4-diamino-diphenylsulphone in combination with isoniazid and rifampicin (author's transl)]. 1975810790
experimental leprosy in the nine-banded armadillo. 1975813256
editorials: problems in identifying m. leprae. 1975815182
preliminary taxonomic studies on the leprosy bacillus.antigens extracted from leprosy bacilli obtained from infected human and armadillo tissues have been examined by immunodiffusion analysis with serum samples from lepromatous patients and with immune sera raised in rabbits. using the best combinations of serum and antigen extracts, 12 antigenic constituents were found in the leprosy bacilli. six of these were antigens common to all mycobacteria and nocardiae, 4 were specific to the leprosy bacillus and the position of 2 could not be determined. g ...1975816369
binding of 14c-labeled dopa by mycobacterium leprae in vitro. 1976819383
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