Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| the effect of different strontium concentrations on the efficacy of chlorhexidine-fluoride-strontium gel in preventing enamel softening in vitro. | the effects were compared of strontium concentrations of 0, 15, 50 and 250 parts/10(6) in chlorhexidine(0.20%)-fluoride(0.16%) gel in preventing the softening of bovine enamel during bacterial fermentation. fresh streptococcus sobrinus cells were suspended in a buffer solution (ph 6.0) with 3.3% sucrose and centrifuged as a plaque-like layer on the top of enamel slabs covered with 10 microliters gel. after 24 h incubation, the ph fall of the fluid was significantly smaller in all test groups tha ... | 1993 | 8476339 |
| immune responses to streptococcus sobrinus surface protein antigen a expressed by recombinant salmonella typhimurium. | in this study, we used a vaccine strain of salmonella typhimurium to express antigenic determinants of the spaa antigen of streptococcus sobrinus, which is involved in the caries-forming process. we cloned either a single repeat (pya2901) or three tandem repeats (pya2905) of the 0.48-kb fragment of the spaa gene, which codes for an important component of the spaa protein, plus a 1.2-kb minor antigenic determinant and measured the resulting immune responses to spaa in orally immunized balb/c mice ... | 1993 | 8478075 |
| nucleotide sequence analysis of the gtft gene from streptococcus sobrinus omz176. | the gtft gene and its upstream region isolated from the streptococcus sobrinus omz176 chromosomal dna were sequenced. the gtft gene was preceded by a potential shine-dalgarno sequence. the gtft gene product, glucosyltransferase (gtf), displays a typical gram-positive bacterial signal peptide sequence and both an active site peptide sequence and carboxy-terminal repeats typical of gtfs. the signal sequence is similar to those of other known gtf proteins. the putative active-site peptide sequence ... | 1993 | 8478099 |
| inhibitory effect of oolong tea polyphenols on glycosyltransferases of mutans streptococci. | oolong tea extract (ote) was found to inhibit the water-insoluble glucan-synthesizing enzyme, glucosyltransferase i (gtase-i), of streptococcus sobrinus 6715. the gtase-inhibitory substance in the ote was purified successive adsorption chromatography on diaion hp-21 and hp-20 columns; this was followed by further purification by sephadex lh-20 column chromatography. a major fraction that inhibited gtase activity (fraction otf10) was obtained, and the chemical analysis of otf10 indicated that it ... | 1993 | 8489234 |
| sequence analysis of scra and scrb from streptococcus sobrinus 6715. | the complete nucleotide sequences of streptococcus sobrinus 6715 scra and scrb, which encode sucrose-specific enzyme ii of the phosphoenolpyruvate-dependent phosphotransferase system and sucrose-6-phosphate hydrolase, respectively, have been determined. these two genes were transcribed divergently, and the initiation codons of the two open reading frames were 192 bp apart. the transcriptional initiation sites were determined by primer extension analysis, and the putative promoter regions of thes ... | 1993 | 8500898 |
| simultaneous caries induction and calculus formation in rats. | weanling specific pathogen-free osborne-mendel rats were fed a high-calcium, high-phosphorus diet with various levels of sucrose and inoculated with streptococcus sobrinus strain 6715-13wt and actinomyces viscosus strain omz-105 in order to determine whether calculus and caries could develop simultaneously. rats consumed diets designated rc-16-5, rc-16-25, or rc-16-50 which partially replaced the corn starch component with progressively higher levels of sucrose, thus, to 5, 25, or 50% sucrose. i ... | 1993 | 8501282 |
| effects of fluoxetine on the oral environment of bulimics. | the purpose of this study was to evaluate in a double-blind placebo-based study the effects of fluoxetine over a period of 16 weeks on the frequency of binging and purging and on fluctuations in the levels of cariogenic organisms and saliva secretion rate of patients (n = 30) with bulima nervosa. profile analysis suggested that, over the course of the study, binging and purging frequency and streptococcus sobrinus salivary levels decreased significantly in the fluoxetine group as compared with t ... | 1993 | 8510986 |
| antigenicity and immunogenicity of a synthetic peptide derived from a glucan-binding domain of mutans streptococcal glucosyltransferase. | the immunogenicity and antigenicity of a multiply antigenic peptide construct containing four copies of the synthetic peptide tgaqtikgqklyfkangqqvkg were measured in rodents and humans, respectively. the composition of this peptide construct (termed glu) was derived from a major repeating sequence in the c-terminal region of mutans streptococcal glucosyltransferases that synthesize water-insoluble glucan (gtf-i). the glu peptide elicited high levels of serum immunoglobulin g antibody to glu afte ... | 1993 | 8514393 |
| sucrose retention and colonization by mutans streptococci at different sites of the dentition. | the correlations between the retention and elimination of sucrose at different oral sites and the colonization by mutans streptococci at the same sites were tested in 10 subjects with > 10(5) mutans streptococci per millilitre of saliva. paraffin-stimulated saliva was collected and plaque samples were taken with a toothpick from all the buccal surfaces except the third molars. one week after the plaque sampling, the sucrose clearance was determined. after a mouth rinse with 10 ml of a 10% sucros ... | 1995 | 8521442 |
| acid production by human strains of streptococcus mutans and streptococcus sobrinus. | acid production by washed suspensions of human strains of streptococcus mutans (n = 18) and streptococcus sobrinus (n = 12) was measured. the strains were isolated from infants and adolescents with varying caries experience. some of these strains and two laboratory strains (1 s. mutans and 1 s. sobrinus) had been tested in an earlier study for their cariogenicity in hamsters. further, 3 streptococcus sanguis strains and 1 s. sobrinus laboratory strain were included. acid production was determine ... | 1995 | 8521443 |
| insertional inactivation of the streptococcus mutans dexa (dextranase) gene results in altered adherence and dextran catabolism. | streptococcus mutans is able to synthesize extracellular glucans from sucrose which contribute to adherence of these bacteria. extracellular dextranase can partially degrade the glucans, and may therefore affect virulence of s. mutans. in order to isolate mutants unable to produce dextranase, a dna library was constructed by inserting random sau3ai-digested fragments of chromosomal dna from s. mutans into the bamhi site of the streptococcal integration vector pva891, which is able to replicate i ... | 1995 | 8535521 |
| comparison of cell sediment and surface grown "test plaque" using scanning electron microscopy. | scanning electron microscopy was used to compare the morphology, integrity and distribution of bacterial cells in a test plaque grown on the surface of enamel with that of the cell sediment plaque routinely used in a short-term intraoral caries model. cultures of s. mutans ib-1600 or s. sobrinus 6715-13 were grown in complex media supplemented with either 2.0% sucrose (glucan plaque) or 0.2% glucose (non-glucan plaque). cell sediment (cs) plaque was prepared by centrifuging the cultures after in ... | 1995 | 8553018 |
| plaque composition, fluoride tolerance and acid production of mutans streptococci before and after the suspension of the use of fluoride toothpastes. | although fluoride toothpastes are widely used for caries prevention, little is known about the impact of fluoride dentifrices on plaque composition. also the issue of adaptation of mutans streptococci to grow in vivo in a fluoride environment has received little attention. such an adaptation may be of interest as it has been suggested that adapted mutans streptococci may show reduced glycolytic activity thereby being less cariogenic. in the present experiments the impact of the suspension of the ... | 1995 | 8556746 |
| adherence of microorganisms to rat salivary pellicles. | numerous studies, using models to mimic the formation of the acquired pellicle, have concentrated on human saliva-coated hydroxyapatite; in contrast, although the rat is frequently used as an animal model, the information concerning the formation of pellicles from rat saliva is sparse. we compared the ability of streptococcus and actinomyces species to adhere to hydroxyapatite coated by rat saliva (rsha) and human saliva (sha). we also explored the influence of glucan synthesized in situ on the ... | 1995 | 8556756 |
| m39 construction of a mobilizable vector for genetic analysis of streptococcus sobrinus. | 1995 | 8586210 | |
| [the local immunity of the oral cavity in dental caries]. | functional activity of local immunity with regard to dental caries in children is liable to appreciable individual fluctuations which depend on sex, age, and season. in children aged 4 to 5 no association between the titer of antibodies to cariogenic streptococcus and number of carious lesions was observed, whereas in those aged 9 to 19 these parameters were in inverse relationship. in children aged 11-12 and 14-16 this association is inverse. regression analysis of nonspecific and specific char ... | 1995 | 8597190 |
| microbial induction of dentinal caries in human teeth in vitro. | the ability of lactobacillus casei, streptococcus sobrinus, actinomyces viscosus, and streptococcus salivarius to induce dental caries was determined in vitro. a class i cavity (depth: 2mm) was prepared in extracted human caries-free premolars to make dentin blocks. the blocks were inoculated with these four bacterial strains in a monoinfective fashion and were incubated under anaerobic conditions. in addition to the monoinfection groups, mixed-infection groups of l. casei with s. sobrinus or a. ... | 1995 | 8601764 |
| the action of thymol on oral bacteria. | several effects of thymol, a plant-derived antimicrobial agent, on porphyromonas gingivalis, selenomonas artemidis and streptococcus sobrinus were examined. the extremely rapid efflux of intracellular constituents evoked by thymol is consistent with its postulated membranotropic effects. correlations between leakage-inducing concentrations of thymol and minimal inhibitory concentrations and minimal bactericidal concentrations suggest that membrane perforation is a principal mode of action of thi ... | 1995 | 8602337 |
| defining antibody targets in streptococcus oralis infection. | immunoblotting of sera from 12 neutropenic patients with streptococcus oralis septicemia and 18 patients with endocarditis due to viridans group streptococci revealed immunodominant s. oralis antigens at 85 and 180 kda. the former cross-reacted with a mouse monoclonal antibody to hsp90. the latter was identified by sequencing positive clones obtained by screening a genomic expression library of s. oralis with pooled sera from patients who had been infected with s. oralis. antibody eluted from on ... | 1996 | 8613367 |
| antibacterial activity of fluoride release sealants on mutans streptococci. | this study was conducted to study the antibacterial activity of sealants on mutans streptococci. the antibacterial effect of fluoride release sealants was estimated by using agar plates infected with several strains of s. mutans and s. sobrinus. both bacterial groups were sensitive to sealant antibacterial activity, there was not statistical difference in the inhibition between both groups. the teethmate-f was the only active material in this study and showed more than four times fluoride releas ... | 1996 | 8619968 |
| multiple glucan-binding proteins of streptococcus sobrinus. | several proteins from culture supernatants of streptococcus sobrinus were able to bind avidly to sephadex g-75. the proteins could be partially eluted from the sephadex by low-molecular-weight alpha-1,6 glucan or fully eluted by 4 m guanidine hydrochloride. elution profiles were complex, yielding proteins of 16, 45, 58 to 60, 90, 135, and 145 kda, showing that the wild-type strain possessed multiple glucan-binding proteins. two mutants of streptococcus sobrinus incapable of aggregation by high-m ... | 1996 | 8626283 |
| cariogenicity depends more on diet than the prevailing mutans streptococcal species. | this review aims to compare the occurrence and distribution of mutans streptococci in africa, europe, and north america and in addition will try to offer explanations for existing relationships among salivary mutans streptococci counts, dietary patterns, and dental caries. the literature reveals that salivary mutans streptococci counts in child populations of the three continents are comparable. the distribution of mutans streptococci species, with a predominance of s. mutans followed by s. sobr ... | 1996 | 8655757 |
| the inhibiting effect of aqueous azadirachta indica (neem) extract upon bacterial properties influencing in vitro plaque formation. | the purpose of this investigation was to examine the inhibitory effects of aqueous extracts derived from the bark-containing sticks (neem stick) of azadirachta indica upon bacterial aggregation, growth, adhesion to hydroxyapatite, and production of insoluble glucan, which may affect in vitro plaque formation. neem stick extracts were screened for minimal bacterial growth inhibition (mic) against a panel of streptococci by means of a broth dilution assay. initial bacterial attachment was quantifi ... | 1996 | 8655780 |
| sequence analysis of the streptococcus mutans ingbritt dexa gene encoding extracellular dextranase. | the complete nucleotide sequence (3,747 bp) of the dextranase gene (dexa) and flanking regions of the chromosome of streptococcus mutans ingbritt (serotype c) were determined. the open reading frame for dexa was 2,550 bp, ending with a stop codon tga. a putative ribosome-binding site, promoter preceding the start codon, and potential stem-loop structure were identified. the presumed dextranase protein (dexa) consisting of 850 amino acids was estimated to have a molecular size of 94,536 da and a ... | 1995 | 8657012 |
| characterization of a p1-deficient strain of streptococcus mutans that expresses the spaa protein of streptococcus sobrinus. | the streptococcus sobrinus spaa protein and the streptococcus mutans p1 protein share 66% sequence homology at the amino acid level. to determine if the spaa protein can be expressed in s. mutans and functionally replace the p1 protein, the spaa gene of s. sobrinus 6715 was isolated from plasmid px1303 and inserted into the escherichia coli-streptococcus shuttle vector pva838. the resulting plasmid px1600 was transformed into the p1-deficient strain s. mutans 834 that has defects in saliva-media ... | 1996 | 8751913 |
| [adherent mechanism of the mutans of actinomyces viscosus t14v]. | three mutans of actionmyces viscous t14v lacking type1 (5951), type 2 (5519) and both types of fimbriae (147) were selected in this study. we compared the ability of their adsorption to the test-tube wall, the result of the cellular coaggregation between these mutant strains and streptococcus sanguis 34, streptococcus sanguis black bova, streptococcus mutans ingbritte, streptococcus sobrinus 6715 and analysed the chemical characterization of cell walls of the three mutans. we found that the spec ... | 1995 | 8762533 |
| peroxidase-catalyzed generation of catechin oligomers that inhibit glucosyltransferase from streptococcus sobrinus. | oolong tea extract (ote) and the purified polymeric polyphenols from ote have been found to inhibit glucosyltransferase (gtase) of mutans streptococci. in view of the partial fermentation characteristic of oolong tea, we describe here an in vitro model reaction system to produce partially fermented products of d-(+)-catechin or green tea extract (gte) using horseradish peroxidase. a dimeric catechin molecule was identified as dehydro-dicatechin a by instrumental analyses. the molecular size of s ... | 1996 | 8807799 |
| caries prevalence and salivary and microbial conditions in 88-year-old swedish dentate people. | as part of a comprehensive investigation of 88-year-old people, caries prevalence, stimulated salivary secretion rate, buffer capacity, and the prevalence of lactobacilli and mutans streptococci were studied in a subsample of 92 dentate subjects. the mean number of remaining teeth was 14.1 +/- 7.3. the dmft was 25.2 +/- 3.0, and df surfaces 38.3 +/- 22.7. root caries experience was found in 85% of the subjects, with a mean root caries index of 36.6 +/- 28.5%. the men had significantly higher mea ... | 1996 | 8811143 |
| inhibitory effects of funoran on the adherence and colonization of mutans streptococci. | funoran, a sulfated polysaccharide extracted from the seaweed gloiopeltis furcata strongly inhibited the absorption of mutans streptococci to saliva-coated hydroxyapatite (s-ha), but enhanced that of streptococcus sanguis atcc 10556 and streptococcus oralis atcc 10557. furthermore, funoran had a strong desorption activity against mutans streptococci preadsorbed to s-ha. in the presence of sucrose, the absorption of cells of streptococcus sobrinus b13, streptococcus mutans ingbritt, and s. mutans ... | 1996 | 8833135 |
| oral immunization with recombinant salmonella typhimurium expressing surface protein antigen a (spaa) of streptococcus sobrinus: effects of the salmonella virulence plasmid on the induction of protective and sustained humoral responses in rats. | recombinant strains of salmonella typhimurium have been studied as antigen delivery systems to determine their effectiveness as multivalent vaccines. here we compare the efficacy of two strains of s. typhimurium. chi 4072 (pya2905) and chi 3987 (pya2905), expressing spaa of streptococcus sobrinus 6715 as oral vaccines for dental caries. both strains are attenuated delta cya delta crp delta asd mutants with their asd phenotypes complemented by the asd+ plasmid pya2905, which also encodes a peptid ... | 1996 | 8843628 |
| effect of copper co-crystallized with sugar on caries development in desalivated rats. | the purpose of the present study was to explore the effects of varying levels of copper incorporated in sucrose by co-crystallization on caries development in the desalivated rat model and to examine the retention of copper in the oral cavity and its influence on protein and carbohydrate composition of plaque. forty-eight sprague-dawley rats were infected by streptococcus sobrinus 6715 and desalivated when aged 26 days. four groups were placed in a könig-höfer programmed feeder and received 17 m ... | 1996 | 8877091 |
| kinetic properties of glucosyltransferase adsorbed onto saliva-coated hydroxyapatite. | results from previous studies have shown that several properties of glucosyltransferase (gtf) adsorbed onto saliva-coated hydroxyapatite beads differ from those of gtf in solution. for example: thermostability, ph-activity dependency, sensitivity to inhibitors. the aim of this study was to compare the kinetics of the adsorbed gtf with its kinetic properties in solution. hydroxyapatite beads were coated with human parotid saliva (sha). following washes, cell-free gtf enzyme from streptococcus sob ... | 1996 | 8879428 |
| anti-adhesins of streptococcus sobrinus. | 1996 | 8895800 | |
| mutans streptococci caries and chlorhexidine. | mutans streptococci, particularly streptococcus mutans and streptococcus sobrinus, can be shown to be highly associated with caries in humans. together with lactobacillus spp., they are regarded as significant odontopathogens. because of their association with dental disease, an evaluation of the numbers of mutans streptococci organisms in plaque and saliva may aid in the diagnosis of caries activity. in conjunction with this concept, control and prevention of caries has been sought by reducing ... | 1996 | 8908872 |
| effect of low-dose glucocorticoid treatment on dentin formation and dentinal caries in rats. | the effect of a low dose of glucocorticoids on dentin formation and progression of dental caries was studied in young rats. the animals were inoculated in the mouth with streptococcus sobrinus and fed ad libitum a high-sucrose, a high-starch, or a reference diet. the cortisone or placebo pellet was implanted subcutaneously in the back of the necks of the animals. after a 6-week experimental period the areas of dentin formation and dentinal caries were quantified planimetrically. schiff's stainin ... | 1996 | 8923921 |
| in vitro evaluation of antibacterial effects of photo-cured glass ionomer liners and dentin bonding agents during setting. | two dentin bonding agents (optibond and syntac), an enamel bonding agent (heliobond), and two photo-cured glass ionomer cements (vitremer and variglass vlc) and their inhibitory effects on bacterial growth during setting were examined. cultures of eight bacterial species were used to test these materials, except for vitremer glass ionomer cement, which was tested against only five species. the photo-cured glass ionomer cements and the syntac dentin bonding system with glutaraldehyde demonstrated ... | 1996 | 8933437 |
| the establishment of a community of oral bacteria that controls the growth of candida albicans in a chemostat. | the purpose of this study was to establish and identify a community of oral bacteria that controls the growth of candida albicans in the chemostat. the chemostat was operated under glucose-limiting conditions at a dilution rate of 0.05 h-1 and inoculated with a yeast-free suspension of a tongue scraping. after a steady state had been reached, it was inoculated with c. albicans to establish the yeast and determine whether its growth could be contained. the steady-state community comprised the spe ... | 1996 | 8941776 |
| incorporation of caseinoglycomacropeptide and caseinophosphopeptide into the salivary pellicle inhibits adherence of mutans streptococci. | the protective effects of milk and milk products against dental caries have been demonstrated in many animal studies. we have shown that this effect was mediated by micellar casein or caseinopeptide derivatives. a reduction in the streptococcus sobrinus population in the oral microbiota of animals fed diets supplemented with these milk components was consistently observed. a possible explanation for these findings is that milk components are incorporated into the salivary pellicle, thereby reduc ... | 1996 | 8955673 |
| effect of antibodies on the chain length and growth of streptococcus sobrinus. | passive immunization has been suggested as a method to prevent colonization of teeth by mutans streptococci. however, the mechanism of action of antibodies, both polyclonal and monoclonal, is not clear. in this study we investigated the effect of polyclonal antibodies (pabs) and a monoclonal antibody (omvu10) on the chain length and growth of streptococcus sobrinus. during growth in broth s. sobrinus formed significantly longer chains in the presence of pabs in comparison to pre-immune serum (p ... | 1997 | 8955992 |
| cariostatic effect of aspartame in rats. | this study examined the effects of aspartame (apm) on caries and the streptococcus sobrinus population. six groups of rats (20 in each group) colonized with s. sobrinus were fed varying amounts of sucrose (su) and apm in their diets as follows: 1-0.15% apm, 2-0.30% apm, 3-30% su, 4-30% su + 0.15% apm, 5-50% su and 6-50% + 0.15% apm. ten from each group were sacrificed at 6 weeks and 10 at 12 weeks. s. sobrinus populations at both intervals were negligible in groups 1 and 2 and had no differences ... | 1997 | 8955999 |
| effect of antibodies on chemiluminescence and on killing of streptococcus sobrinus by polymorphonuclear leukocytes. | the effect of a polyclonal antiserum and omvu10, a monoclonal antibody reactive with antigen b of streptococcus sobrinus, on the interaction of polymorphonuclear leukocytes with s. sobrinus was studied, using chemiluminescence and bacterial killing assays. increased stimulation of neutrophils as measured in the chemiluminescence assays was established when s. sobrinus was preincubated with polyclonal antiserum or when polyclonal antiserum was added to the reaction mixture. higher counts were mea ... | 1996 | 9002878 |
| typing of mutans streptococci by arbitrarily primed polymerase chain reaction. | the discriminative power of the arbitrarily primed polymerase chain reaction (ap-pcr) in differentiating between streptococcus mutans and strep. sobrinus species, serotypes and clones was investigated. mutans streptococcal isolates (12(7)) obtained from 65 individuals (1-10 isolates per individual) were ap-pcr typed separately with two random primers, opa-05 and opa-13. bacterial cell lysates were used as a template in pcr reactions, which made ap-pcr easy and fast to perform. eighty-one isolate ... | 1996 | 9022919 |
| the inhibitory action of fatty acids on oral bacteria. | saturated and unsaturated fatty acids and fatty acid derivatives were examined for their growth-inhibitory effects towards three selected oral bacteria: porphyromonas gingivalis, selenomonas artemidis, and streptococcus sobrinus. of the 45 compounds surveyed, only one, myristoleic acid, was inhibitory towards s. artemidis at a concentration < 100 micrograms/ml. cis-hexadecenoic and cis-octadecenoic acids were generally inhibitory towards p. gingivalis and s. sobrinus, but there was no correlatio ... | 1996 | 9028262 |
| the phosphoenolpyruvate:sugar phosphotransferase system of oral streptococci and its role in the control of sugar metabolism. | oral streptococci are sugar-fermentative bacteria comprising at least 19 distinct species and are a significant proportion of the normal microbial population of the mouth and upper respiratory tract of humans. these streptococci transport several sugars by the phosphoenolpyruvate:sugar phosphotransferase system (pts) which concomitantly catalyzes the phosphorylation and translocation of mono- and disaccharides via a chain of enzymic reactions that transfer a phosphate group from phosphoenolpyruv ... | 1997 | 9050218 |
| inhibition by hop bract polyphenols of cellular adherence and water-insoluble glucan synthesis of mutans streptococci. | the inhibitory effect of hop bract polyphenols (hbp) on cariogenic streptococci was investigated. it was found that the high molecular weight polyphenol (estimated about 36,000-40,000) inhibited the cellular adherence of streptococcus mutans mt8148 (serotype c) and streptococcus sobrinus atcc 33478 (serotype g) at much small concentrations than the polyphenols extracted from oolong tea or green tea leaves. furthermore, hbp also inhibited the action of glucosyltransferase, which was involved in t ... | 1997 | 9058972 |
| effects of copper, iron and fluoride co-crystallized with sugar on caries development and acid formation in deslivated rats. | the purpose was to explore the effects of combinations of copper, iron and fluoride (cu, fe and f) incorporated in sucrose by co-crystallization on caries development in the deslivated rat model and to examine acid formation by bacteria in the rat mouth. ninety-six sprague-dawley rats were infected with streptococcus sobrinus 6715 and desalivated when aged 26 days. eight groups were placed in a könig-höfer programmed feeder and received 17 meals daily at hourly intervals, and essential nutrition ... | 1996 | 9068864 |
| antibacterial effect of chlorhexidine on bacteria adsorbed onto experimental dental plaque. | the most common method used to examine the antibacterial effect of antiplaque agents is the minimal inhibitory concentration (mic) method, which is tested on bacteria in suspensions. examining the antibacterial effect on bacteria adsorbed onto dental surfaces models is not as popular, although it is clear that such models reflect the conditions in the oral cavity far more accurately than the conventional mic method. the antibacterial effect of chlorhexidine (chx) on bacteria adsorbed onto experi ... | 1996 | 9078445 |
| initial ph as a determining factor of glucose consumption and lactic and acetic acid production in oral streptococci. | lactic and acetic acid production was evaluated from six strains of oral streptococci, viz streptococcus gordonii, streptococcus mutans, streptococcus oralis, streptococcus salivarius, streptococcus sanguis and streptococcus sobrinus cultured in the presence of 1, 5, 10 mm glucose and without glucose, at initial ph values of 5, 5.5, 6 and 7. s. sobrinus and s. salivarius caused the greatest decreases in ph. at ph values of 5 and 5.5, lactic acid and acetic acid production in the species tested w ... | 1996 | 9082145 |
| fluoride and pilocarpine reduce the risk of caries produced by chronic clomipramine treatment in rats. | the effects of chronic clomipramine treatment on the incidence of caries in the rat and their prevention by fluoride and pilocarpine were investigated. one hundred and twenty male wistar rats were divided into six groups, five experimental groups, one control group and treated with 50 mg/kg/day of clomipramine, 10 ppm fluoride in drinking water, and 5 mg/kg/day of pilocarpine. all animals were infected with streptococcus sobrinus 6715 and fed a cariogenic diet ad libitum for 42 days. water and f ... | 1997 | 9118188 |
| chemical and functional properties of mutastein, an inhibitor of insoluble glucan synthesis by streptococcus sobrinus. | mutastein, a potent inhibitor of insoluble glucan synthesis by streptococcus sobrinus, is a protein with a molecular weight of approximately 2 x 10(6). amino acid and elisa analyses suggested that mutastein is a mixture of heterogenous polymers of alpha-casein contained in the culture medium of the producing strain, aspergillus terreus m3328. mutastein strongly inhibited the primer-dependent insoluble glucan synthase of s. sobrinus b13. the primer-independent soluble glucan synthase was not affe ... | 1997 | 9145515 |
| influence of iron alone or with fluoride on caries development in desalivated and intact rats. | sugar is used as a vehicle for iron fortification in communities where anemia is prevalent. the purpose of the present study was to investigate the relationship between caries experience and iron concentration in sugar fed to rats subjected to a severe cariogenic challenge. desalivated and intact animals were assigned to 4 different diet subgroups, 3 receiving iron sucrose (350, 175 and 88 ppm fe) and a control group. the influence of iron sugar combined with 10 ppm f in drinking water was explo ... | 1997 | 9165198 |
| influence of fluoride co-cystallized with sugar on caries development in desalivated rats. | previous studies have shown that fluoride (f) administered concomitantly with sucrose in drinking water, in the diet, or alone is an effective cariostatic agent. the purpose of the present study was to determine the minimum concentration of f co-crystallized with sugar that may be used to prevent dental caries in rats subjected to a severe cariogenic challenge. desalivated sprague-dawley rats, infected with streptococcus sobrinus, were placed in a könig-höfer programmed feeder. the rats received ... | 1997 | 9222451 |
| assessing the cariogenic potential of some infant formulas, milk and sugar solutions. | using a desalivated rat model, the authors found that several commonly used infant formulas had significant cariogenic potential. sucrose was by far the most cariogenic and cows' milk the least cariogenic of all the products examined. the data show that dental practitioners and other health care professionals should discourage the use of sugar in baby bottles and provide information on which formulas are least likely to induce caries when continuous bottle feeding is unavoidable. | 1997 | 9231601 |
| trends in dental health among icelandic urban children. | caries experience, oral hygiene and caries-related salivary parameters were recorded in a 20% representative sample of 12-year-old schoolchildren in reykjavik, iceland in 1991. the majority of the children was re-examined 3 years later in 1994. trends in prevalence of caries and salivary bacteria were assessed by comparison with an analogous earlier longitudinal study (1984-87). mean dfs values for 12-year-olds were 12.1 and 4.1, for 15-year-olds 23.3 and 11.3 in the earlier and later study, res ... | 1997 | 9249184 |
| caries and plaque inhibition in rats by five topically applied dentifrices. | the effectiveness of 5 different brands of dentifrices on caries and plaque were tested in rats in 2 experiments. 10 litters of rats each were infected with streptococcus sobrinus omz-176 and actinomyces viscosus ny1. the rats were offered the diet 2000a containing either 40% of sucrose (study i) or 10% of sucrose (study ii). the following products were tested topically: (1) h2o, (negative control); (2) chlorhexidine diacetate, (positive control); (3) parodontax nf; (4) meridol; (5) colgate tota ... | 1997 | 9249193 |
| mutans streptococcal serotypes in children with gastroesophageal reflux disease. | it has been suggested that vomiting acid gastric contents in bulimia might favor oral growth of streptococcus sobrinus. we studied the colonization of streptococcus sobrinus (serotypes g and d) and streptococcus mutans (serotypes c, e and f) in sixteen children, ages five to fifteen years, who had suffered for four to eleven years from gastroesophageal reflux, another condition with recurrent acid regurgitation. our aim was to find out if the prevalence of streptococcus sobrinus would be higher ... | 1997 | 9262802 |
| [the effects of lipoteichoic acid and calcium on the adherence of oral streptococci in vitro]. | in this study, the effects of lipoteichoic acid (lta) and calcium on adherence of oral streptococci in vitro were investigated. the results revealed that the adherence of streptococcus sobrinus to a glass surface was increased by the addition of calcium, in the condition of the same calcium ion concentration, lta inhibited adherence, suggesting that lta and the bacterial cell surface lta competed for the same site of calcium ion, providing some evidence to support calcium-bridging theory. the re ... | 1996 | 9275604 |
| generation of bovine immune colostrum against streptococcus mutans and streptococcus sobrinus and its effect on glucose uptake and extracellular polysaccharide formation by mutans streptococci. | due to potential side-effects of active immunization by cariogenic mutans streptococci, oral administration of passively-derived antibodies could be a more acceptable way to reduce colonization and virulence of these microorganisms in human dentition. the aim of this study was to produce antistreptococcal immunoglobulins into bovine colostrum and explore the possible antibacterial mechanisms of these immunoglobulins against mutans streptococci. specific serum igg antibodies to whole cell antigen ... | 1997 | 9286054 |
| mutans streptococci and incipient caries adjacent to glass ionomer cement or resin-based composite in orthodontics. | levels of mutans streptococci in plaque adjacent to orthodontic brackets retained with a glass ionomer cement (gic) (ketac-cem) and a resin-based composite (cr) (concise) were investigated, using the split mouth technique in 11 patients who, before treatment, had more than 10(5) cfu of these microorganisms. after full-term orthodontic treatment (mean 9.5 months), the percentage of mutans streptococci of total cfu count in plaque was lower adjacent to gic-retained brackets (mean 3.9) than adjacen ... | 1997 | 9294355 |
| thermophysiology of streptococcus mutans and related lactic-acid bacteria. | the temperature ranges for growth of streptococcus mutans gs-5 and s. sobrinus 6715 were found to be very narrow, from about 30 to 47 degrees c, with optimal growth around 37 degrees c. thus, the organisms showed little potential to grow in the environment outside of the animal host. in contrast wider ranges were found for enterococcus hirae, s. rattus and s. sanguis. detailed study of s. mutans gs-5 showed that energetic coupling, reflected in yields of biomass per mol of glucose utilized, were ... | 1997 | 9298187 |
| caries-inducing activity of the hydrogenated derivative of an isomaltooligosaccharide mixture in rats. | the caries-inducing activity of the hydrogenated derivative of an isomaltooligosaccharide mixture (imo-h) was evaluated in vitro for its acidogenicity and in vivo an experimental caries system with specific-pathogen-free (spf) rats. streptococcus sobrinus 6715 (serotype g) did not produce a significant amount of acid from imo-h, whereas streptococcus mutans mt8148 (serotype c) gradually produced a small amount of acid, although the degree was less than that of sucrose. in vivo experiments were c ... | 1997 | 9301113 |
| high-level ability of secretory iga to block hiv type 1 transcytosis: contrasting secretory iga and igg responses to glycoprotein 160. | the igg and secretory iga (s-iga) responses to the hiv-1 envelope (gp160 antigen) were analyzed in the colostrum (col) and in the cervicovaginal fluid (cvf) of hiv-l-infected women. we show igg antibodies (abs) to the recombinant gp160 to be predominant as compared with the corresponding s-iga isotype. the low level of the s-iga response cannot be related to a general disturbance of the mucosal-associated iymphoid tissue (malt) because the level of a current ab to a caries-associated antigen fro ... | 1997 | 9310284 |
| adherence of oral streptococci to an immobilized antimicrobial agent. | an antimicrobial agent, 3-(trimethoxysilyl)-propyldimethyloctadecyl ammonium chloride, was immobilized on silica. interaction between the material (termed) oais) and various oral bacterial species were then studied. seven species of streptococcus and two actinomyces were investigated for their ability to adhere to this biomaterial. cell-surface hydrophobicity and zeta-potential were examined as well. analysis of extracted hydrophobic proteins which adhered to oais revealed that the adherence of ... | 1997 | 9347116 |
| immunogenicity and protective immunity induced by synthetic peptides associated with a catalytic subdomain of mutans group streptococcal glucosyltransferase. | we examined the immunogenicity and induction of protective immunity of two 19-mer sequences (ggy and and) which overlapped a highly conserved region which has recently been implicated in the enzymatic activity of glucosyltransferases (gtfs) of the mutans group streptococci. these peptides were synthesized as eight-branched constructs on a lysine core. serum immunoglobulin g (igg) antibody, induced by subcutaneous (s.c. [salivary gland vicinity]) injection with these peptide constructs, reacted w ... | 1997 | 9353015 |
| tonsillar application of killed streptococcus mutans induces specific antibodies in rabbit saliva and blood plasma without inducing a cross-reacting antibody to human cardiac muscle. | when streptococcus mutans cells are injected into the skeletal muscle of rabbits, an antibody against human cardiac muscle, as well as an anti-s. mutans antibody, is induced in blood plasma. our previous study showed that when sheep erythrocytes are applied to palatine tonsils, an antibody against the applied cells is induced both in blood plasma and saliva. this antibody has no activity against cardiac muscle. it is not clear, however, if s. mutans application to the tonsils evokes an antibody ... | 1997 | 9353033 |
| adsorption of streptococcus sobrinus dextranase inhibitor to water-insoluble alpha-d-glucans of oral streptococci. | a low molecular weight dextranase inhibitor from streptococcus sobrinus has previously been identified and purified. the range of conditions under which inhibition occurs, and the situations in which dextranase activity of s. sobrinus can reappear, have been examined in the chemostat. these studies have revealed that when dextranase production exceeds that of the inhibitor, all the inhibitor is tightly bound into enzyme-inhibitor complexes, and the excess enzyme remains active. another factor th ... | 1997 | 9353584 |
| anti-bacterial action of onion (allium cepa l.) extracts against oral pathogenic bacteria. | in this study, the focus was on the antibacterial activity of onions. this study researched the activities of onion extracts on streptococcus mutans and streptococcus sobrinus, the main causal bacteria for dental caries, and porphyromonas gingivalis and prevotella intermedia, considered to be the main causal bacteria of adult periodontitis. the results showed that the onion extracts possess an effect on all test bacterial strains (s.mutans jc-2, s. sobrinus omz176, p. gingivalis atcc 33277 and p ... | 1997 | 9354029 |
| dental caries and related factors in 88- and 92-year-olds. cross-sectional and longitudinal comparisons. | our aim was to compare two groups of 88- and 92-year-olds (n = 92 and n = 40), respectively, with regard to teeth, caries, and salivary and microbial conditions. oral variables were analyzed in relation to functional capacity and use of cardiovascular agents and psychoactive drugs. untreated root caries, plaque score, and counts of lactobacilli increased between the ages of 88 and 92 years (p < 0.01). nine of the 24 longitudinally followed up subjects had lost 1-5 teeth over 4 years, and 17 subj ... | 1997 | 9370025 |
| purification, and biochemical and biological characterization of an immunosuppressive and lymphocyte mitogenic protein secreted by streptococcus sobrinus. | an immunosuppressive/mitogenic (ism) protein was purified from the supernatants of cultures of streptococcus sobrinus with an isoelectric point of 4.75 and a relative molecular mass of 38 kda (p38). treatment of c57bl/6 mice with p38 induced an increase in the numbers of non-specific splenic ig-secreting plaque-forming cells (pfc) with peak responses on day 3 for igm-secreting pfc and on day 5 for igg-secreting pfc, with an isotype pattern consisting predominantly of igg2a and igg2b. this increa ... | 1997 | 9418134 |
| sugar metabolism by mutans streptococci. | 1997 | 9436320 | |
| effects of various resin composite (co)monomers and extracts on two caries-associated micro-organisms in vitro. | previous studies have shown that extracts of various filling materials, e.g., resin composites, may influence the growth of cariogenic micro-organisms. the purpose of this study was to examine the effects of important resin composite (co)monomers (bis-gma, udma, tegdma, egdma) and extracts of two commercial dental composites with similar composition (composite a, arabesk; composite s, superlux) on the growth of the two cariogenic bacterial pathogens streptococcus sobrinus and lactobacillus acido ... | 1998 | 9437400 |
| ecological study of streptococcus mutans, streptococcus sobrinus and lactobacillus spp. at sub-sites from approximal dental plaque from children. | previous immunofluorescence (if) studies have indicated that streptococcus mutans may preferentially colonise specific sub-sites within approximal plaque. the present study aimed to extend these observations to other mutans streptococci and lactobacilli in such gingival margin plaque. two hundred and seventy approximal plaque samples were taken from 90 teeth (3 from each tooth) in 64 children; three gingival margin sub-sites in relation to the contact area: away from (a), to the side of(s) and b ... | 1998 | 9438572 |
| comparison of the cariostatic effects between regimens to administer oolong tea polyphenols in spf rats. | the cariostatic effect of oolong tea polyphenols administered according to several regimens was examined in specific pathogen-free (spf) sprague-dawley rats given both a diet containing 20% sucrose and infected with s. sobrinus 6715. the crude preparation (ote) of oolong tea polyphenols showed the most prominent effect on caries reduction in spf rats when ote was administered in the drinking water beginning 1 day prior to the inoculation of s. sobrinus 6715, when compared with chromatographicall ... | 1998 | 9438575 |
| effects of a hydrogenated isomaltooligosaccharide mixture on glucan synthesis and on caries development in rats. | the caries inhibitory effect of the hydrogenated derivative of an isomaltooligosaccharides mixture (imo-h) was examined in vitro and in vivo experiments. imo-h could not be used as a substrate for the crude glucosyltransferases (gtases) of streptococcus sobrinus 6715 to synthesize water-insoluble glucan. moreover, it not only significantly inhibited the synthesis of water-insoluble glucan from sucrose, but also the sucrose-dependent adherence of these growing cells the glass surfaces. in the in ... | 1997 | 9438982 |
| immunoglobulin a reaction to oral streptococci in saliva of subjects with different combinations of caries and levels of mutans streptococci. | the aim of this study, performed in bangkok, was to study whether a particular salivary immunoglobulin a (iga) antibody profile against mutans streptococci could be related to the absence or presence of caries. a group of 12-year-old individuals representing various combinations of mutans streptococci levels and caries experience was selected. whole saliva stimulated by paraffin-chewing was collected, and the children were investigated for decayed, missing and filled surfaces (dmfs) and teeth (d ... | 1997 | 9467389 |
| cariogenic [correction of carcinogenic] potential of a typical cassava flour from the amazonian region of brazil. | the purpose of this study was to compare the cariogenic potential of a typical cassava flour (cf) with sucrose and starch, using a severe cariogenic challenge model in rats. thirty wistar female pups with their dams (mutans streptococci free) were infected by streptococcus sobrinus 6715, desalivated when aged 25 days, and placed in a konig-hofer programmed feeder at age 26 days. they received 17 meals daily at hourly intervals for 21 days as follows: group (1) powdered plain sucrose and sterile ... | 1997 | 9495154 |
| whole-cell protein electrophoretic analysis of viridans streptococci: evidence for heterogeneity among streptococcus mitis biovars. | one hundred reference strains representing all species belonging to the different phylogenetic lineages of the viridans streptococci were examined by means of one-dimensional whole-organism protein electrophoresis. for most of the species examined, multiple strains characterized by dna-dna hybridization were included and, wherever described, representatives of different biochemical variants were analysed. most species were clearly differentiated. the data support the viewpoint that members of th ... | 1998 | 9542082 |
| root caries and some related factors in 88-year-old carriers and non-carriers of streptococcus sobrinus in saliva. | the prevalence of caries on exposed root surfaces in 88-year-old subjects with and without salivary levels of streptococcus sobrinus was studied. ninety-two individuals were examined with regard to root caries lesions and fillings. the root caries index (rci) was calculated and related to salivary flow rate and buffer capacity, plaque score and salivary counts of streptococcus mutans, s. sobrinus and lactobacilli. in 89 subjects with exposed root surfaces, all but 2 harbored mutans streptococci; ... | 1998 | 9544856 |
| extraction, assay, and analysis of antimicrobials from plants with activity against dental pathogens (streptococcus sp.) | many dental and periodontal diseases are largely a question of bacterial etiology. dental caries develop due to an increase of strongly acidogenic and aciduric gram-positive bacteria while common forms of periodontal disease are linked to anaerobic gram-negative bacteria in subgingival plaque. many plants and plant-derived antimicrobial components are used in folklore therapeutics for the treatment of periodontal disorders and for the purposes of oral hygiene. some have been evaluated for possib ... | 1998 | 9553834 |
| in vitro assessment of the antimicrobial activity of a local sustained release device containing amine fluoride for the treatment of oral infectious diseases. | dental caries and periodontal diseases are chronic infectious diseases caused by oral bacteria. local sustained release delivery systems extend the time in which the drug is present in the oral cavity, thus enhancing its therapeutic potential while reducing its side effects. amine-fluorides (amf) are known anticaries agents and have recently been found to have an antibacterial effect against periodontal pathogens and caries-associated bacteria. the purpose of this in vitro study was to assess th ... | 1998 | 9554175 |
| stabilization of the glucan-binding lectin of streptococcus sobrinus by specific ligand. | cell suspensions of streptococcus sobrinus can be aggregated by high molecular-weight alpha-1,6 glucans. the aggregation depends on the fidelity of a cell wall-bound, glucan-binding lectin (gbl). it is thought that the lectin may play a part in the sucrose-dependent accretion of streptococci in dental plaques. results showed that the anionic detergent, sodium dodecyl sulphate (sds) was a potent inhibitor of the lectin. when cells were incubated in sds and washed to remove the detergent, lectin a ... | 1998 | 9569988 |
| rapid isolation of chromosomal dna from oral streptococci and polymerase chain reaction-oriented restriction fragment-length polymorphism analysis for genetic heterogeneity. | we have extensively modified the published method for the lysis of gram-positive bacteria to isolate chromosomal dna from only 1 ml of oral streptococcal overnight culture. cells were incubated with lysozyme and r nase a in the presence of polyethylene glycol. after centrifugation, cells were lysed with sodium dodecyl sulfate and proteinase k. following ethanol precipitation, sodium dodecyl sulfate solution was added to the residue, and the pellet was completely dispersed by incubating at 65 deg ... | 1998 | 9573816 |
| five-year incidence of caries, salivary and microbial conditions in 60-, 70- and 80-year-old swedish individuals. | the 5-year incidence of dental caries in a random sample of 60-, 70- and 80-year-old inhabitants of goteborg was related to salivary and microbial conditions. of the 208 persons examined at baseline, 148 (71%) participated in the follow-up examination; 69, 51 and 28, respectively, in the different age groups. the study revealed that 27% of the participants had not developed any carious lesions during the 5-year period and that the incidence of coronal and root caries increased with age. in the 6 ... | 1998 | 9577981 |
| [expression of the pac gene of s. mutans in s. lactis]. | expression of the pac gene in s. lactis were analyzed by dot immunoblotting with rabbit anti-pac serum. the amount of pac produced by s. lactis hl107 (plf107) was measured. this result indicated that a small amount of rpac was detected in the culture supernatant and cell homogenate of s. lactis hl107 (plf107) but not in the control strain s. lactis lm0230. the amount of rpac in s.lacits hl107 (plf107) was six times lower than that in s. mutans ingbritt. expression of cloned pac gene in s. lactis ... | 1996 | 9592280 |
| mutacin production by streptococcus mutans may promote transmission of bacteria from mother to child. | the production of bacteriocin-like inhibitory substances, mutacins, by mutans streptococci varies among isolates. to find if the degree of mutacin activity of an isolate was related to its transmission between mother and her child, 19 mothers and their 18-month- to 3-year-old children were sampled for their oral mutans streptococci. in addition, the stability of mutacin activity was studied with isolates from the mothers and with isolates from five unrelated 5-year-old children in 5- to 7-year f ... | 1998 | 9596721 |
| demonstration of identical strains of mutans streptococci within chinese families by genotyping. | the aim of this study was to investigate the intra-familial distribution of mutans streptococci in some chinese families. eighteen families consisting of mother, father and a 3-yr-old child without any older siblings participated. clinical examination and interview were performed to obtain information about level of mutans streptococci in saliva, caries prevalence scored by dmft or deft, general health, diet regimens, breast-feeding time, principal caretaker of the child and the parents' profess ... | 1998 | 9672101 |
| study of root caries in an artificial mouth. | the object of this project was to develop a laboratory model system to study root and enamel caries. model plaque biofilms containing consortia of four (streptococcus mutans, streptococcus sobrinus, actinomyces naeslundii, and lactobacillus rhamnosus) or six (s mutans, s sobrinus, s mitis, a naeslundii, l rhamnosus, and veillonella dispar) species of caries pathogens were developed in a artificial mouth. caries lesions similar to those found in vivo were produced on enamel and dentine blocks and ... | 1998 | 9676475 |
| the activation and function of host matrix metalloproteinases in dentin matrix breakdown in caries lesions. | matrix metalloproteinases (mmps) are a family of enzymes which, in concert, are capable of degrading collagen. we investigated whether human mmps could participate in the degradation of dentin organic matrix after demineralization. we performed western blot analyses using mmp-specific antibodies to identify mmps in human dental caries lesions. enzymography and functional activity assays, with 125i-labeled gelatin as substrate or quantitating the degradation of type i collagen, were used to deter ... | 1998 | 9719036 |
| structural and antigenic characteristics of streptococcus sobrinus glucan binding proteins. | three purified glucan binding proteins (gbp-2, gbp-3, and gbp-5) from streptococcus sobrinus 6715 were compared structurally by mass spectroscopy of tryptic fragments and antigenically by western blot analysis with rat antisera to each gbp or to peptides containing putative glucan binding epitopes of mutans streptococcal glucosyltransferases. structural and antigenic analyses indicated that gbp-3 and gbp-5 are very similar but that both are essentially unrelated to gbp-2. none of these s. sobrin ... | 1998 | 9784575 |
| acid-regulated proteins induced by streptococcus mutans and other oral bacteria during acid shock. | our previous research has demonstrated that with the more aciduric oral bacteria, an acid shock to sub-lethal ph values results in the induction of an acid tolerance response that protects the cells at extremely low ph (ph 3.0-4.0) that kills unadapted control cells maintained at ph 7.5 (oral microbiol immunol 1997: 12: 266-273). in this study, we were interested in comparing the protein profiles of acid-shocked and control cells of nine organisms from three acid-ogenic genera that could be cate ... | 1998 | 9807121 |
| bacterial adherence to bleached surfaces of composite resin in vitro. | the effect of bleaching agents on bacterial adherence to polished surfaces of composite resin restorations was assessed in vitro. | 1998 | 9830652 |
| secretory immunity in defense against cariogenic mutans streptococci. | specific immune defense against cariogenic mutans streptococci is provided largely by salivary secretory iga antibodies, which are generated by the common mucosal immune system. this system is functional in newborn infants, who develop salivary iga antibodies as they become colonized by oral microorganisms. the mechanisms of action of salivary iga antibodies include interference with sucrose-independent and sucrose- dependent attachment of mutans streptococci to tooth surfaces, as well as possib ... | 1999 | 9831775 |
| concentrated bovine colostral whey proteins from streptococcus mutans/strep. sobrinus immunized cows inhibit the adherence of strep. mutans and promote the aggregation of mutans streptococci. | the aim of this study was to examine the effect of bovine colostral whey proteins from cows immunized with streptococcus mutans/strep. sobrinus on the adherence and aggregation of caries-inducing bacteria, i.e., mutants streptococci. both adherence and aggregation are important phenomena in the bacterial colonization of the human oral cavity. in all adherence experiments there was a significant difference between treatments by immune product (ip; from immunized cows) and a control product (cp; a ... | 1998 | 9839215 |
| effects of continuous glucocorticoid infusion on the progression of dentinal caries in growing rats. | this study was undertaken to test the effects of a low dose of continuous glucocorticoid infusion on the rate of progression of dentinal caries in molars of young rats. forty-seven rats were inoculated in the mouth with streptococcus sobrinus and fed ad libitum a cariogenic diet and 10% sweetened water. after 10 days of caries initiation ten animals were killed to serve as a reference group. in the rest of the animals the cortisone or placebo pellet was implanted subcutaneously in the back of th ... | 1998 | 9860095 |
| comparison of dentin apposition and dentinal caries progression in the mandibular and maxillary molars of the rat. | to show that the rate and the rate of reduction of dentin apposition are about the same in mandibular and maxillary molars, 23 sprague-dawley rat pups were randomized into 2 groups on the day of birth. during lactation half of their dams received a standard rodent diet; the other half, a diet containing 41% sucrose. at the age of 3 weeks the pups were weaned, weighed, given an intraperitoneal injection of oxytetracycline hydrochloride, and inoculated with oral streptococcus sobrinus. during the ... | 1998 | 9860098 |
| tonsillar application of formalin-killed cells of streptococcus sobrinus reduces experimental dental caries in rabbits. | living streptococcus sobrinus cells were orally inoculated into nonimmune rabbits and rabbits immunized with formalin-killed cells of s. sobrinus through tonsillar application to examine the anticaries potential of this method of immunization. the living s. sobrinus cell numbers and the caries areas in the rabbits immunized by tonsillar application decreased to a level one-fifth of that in nonimmune rabbits. | 1999 | 9864248 |
| estimation of the caries-related risk associated with infant formulas. | baby bottle tooth decay (bbtd) affects 6% of children under three years of age and is associated with inappropriate bottle use. the objective of this study was to estimate the caries-related risk associated with 26 infant formulas and whole milk. | 1998 | 9866143 |
| combined inhibitory effect of bovine immune whey and peroxidase-generated hypothiocyanite against glucose uptake by streptococcus mutans. | immune whey product was obtained from streptococcus mutans- and streptococcus sobrinus-immunized cows. hypothiocyanite (hoscn/oscn-) was generated in vmg-buffer, ph 5.5 or 6.5, by bovine milk lactoperoxidase, kscn and hydrogen peroxide. the glucose incorporation by late-log cells of s. mutans 10449, serotype c, was followed by measuring the uptake of 14c-glucose at 37 degrees c. at ph 5.5 and 6.5 both immune whey product and hoscn/oscn- dose-dependently inhibited glucose uptake. the inhibition b ... | 1998 | 9872115 |
| the effects of fluoride concentration and the level of cariogenic challenge on caries development in desalivated rats. | dental caries is an infectious and transmissible disease that continues to affect the majority of people. the presence of carbohydrate, mainly sucrose in the diet, is an important factor in its occurrence. the amount of fluoride required for optimal protective effect where there is a high caries challenge is unclear. differences in the intensity of cariogenic challenge, for whatever reason, may play a part in determining fluctuations in the effectiveness of fluoride. the purpose of this study wa ... | 1998 | 9877322 |
| litter effects on caries in rats and implications for experimental design. | the purpose of this study was to obtain quantitative estimates of litter effects on caries development in rats and to examine the implications for design of rat caries experiments. twelve female sprague-dawley rats, aged 60 days, were bred with 4 male rats. nine of the 12 dams had litters in close proximity. the litters were culled to 10 pups. one pup from each litter was placed with each of the other dams for nursing, leaving 2 pups from each litter with the birth dam. this design allowed the l ... | 1999 | 9892785 |