Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| kingella kingae: an emerging pathogen in young children. | kingella kingae is being recognized increasingly as a common etiology of pediatric osteoarticular infections, bacteremia, and endocarditis, which reflects improved culture methods and use of nucleic acid-amplification techniques in clinical microbiology laboratories. k kingae colonizes the posterior pharynx of young children and is transmitted from child to child through close personal contact. day care attendance increases the risk for colonization and transmission, and clusters of k kingae inf ... | 2011 | 21321033 |
| fastidious gram-negatives: identification by the vitek 2 neisseria-haemophilus card and by partial 16s rrna gene sequencing analysis. | taxonomy and identification of fastidious gram negatives are evolving and challenging. we compared identifications achieved with the vitek 2 neisseria-haemophilus (nh) card and partial 16s rrna gene sequence (526 bp stretch) analysis with identifications obtained with extensive phenotypic characterization using 100 fastidious gram negative bacteria. seventy-five strains represented 21 of the 26 taxa included in the vitek 2 nh database and 25 strains represented related species not included in th ... | 2010 | 21347215 |
| microbiological profile of haematogenous osteoarticular infections in children. | introduction: staphylococcus aureus, kingella kingae and ß-hemolytic streptococcus are presently the most frequently identified bacteria in child haematogenous osteoarticular infection. objective: to determine the microbiological profile (bacteria and antibiotic susceptibility) of osteoarticular infections in a paediatric hospital, so as to adapt treatment protocols to the ecology of the bacteria isolated. patients and methods: prospective descriptive study, including children admitted for acute ... | 2011 | 21371961 |
| cytotoxic effects of kingella kingae outer membrane vesicles on human cells. | kingella kingae is an emerging pathogen causing osteoarticular infections in pediatric patients. electron microscopy of k. kingae clinical isolates revealed the heterogeneously-sized membranous structures blebbing from the outer membrane that were classified as outer membrane vesicles (omvs). omvs purified from the secreted fraction of a septic arthritis k. kingae isolate were characterized. among several major proteins, k. kingae omvs contained virulence factors rtxa toxin and pilc2 pilus adhes ... | 2011 | 21443941 |
| kingella kingae spondylodiscitis in young children: toward a new approach for bacteriological investigations? a preliminary report. | as the result of improved bacteriological techniques, kingella kingae is a slow-growing gram-negative coccobacillus that is emerging as an important cause of spondylodiscitis in children younger than 3 years of age. the high pharyngeal carrier rates of this slow-growing gram-negative coccobacillus combined with the low incidence of identified k. kingae infections is possibly explained by a low virulence of this bacterium. the use of specific real-time polymerase chain reaction (pcr) on blood sam ... | 2010 | 21455474 |
| [kingella kingae infections in the pediatric age]. | kingella kingae infections have aroused great interest in the last few years because of the increasing number of identified cases. although considered an emerging pathogen, the increase in diagnosis of these infections can probably be explained by better knowledge of the bacteria, improved microbiological diagnostic techniques and greater awareness among clinicians. k. kingae is an aerobic cocobacillus with high tropism for osteoarticular tissue, endocardium, and vascular space. this pathogen ma ... | 2011 | 21458708 |
| differentiating osteoarticular infections caused by kingella kingae from those due to typical pathogens in young children. | osteoarticular infections caused by kingella kingae are characterized by mild-to-moderate clinical and biologic inflammatory signs that are different from those caused by gram-positive cocci. a combined score was built to find the best model to predict k. kingae osteoarticular infections by using the following 4 variables: body temperature <38°c, serum c-reactive protein <55 mg/l, white blood cell count <14,000/mm, and band forms <150/mm. | 2011 | 21494171 |
| comparison of clinical and biologic features of kingella kingae and staphylococcus aureus arthritis at initial evaluation. | we conducted a retrospective study comparing the presenting clinical and biologic features of 64 children who had septic arthritis caused by kingella kingae with 26 children who had septic arthritis caused by staphylococcus aureus. children with k. kingae septic arthritis were significantly younger than those with s. aureus septic arthritis. otherwise, there were no significant differences between the 2 groups with respect to fever, location, white blood cell count, synovial fluid cell count, c- ... | 2011 | 21552181 |
| broad spectrum biofilm inhibition by kingella kingae exopolysaccharide. | cell-free extracts prepared from kingella kingae colony biofilms were found to inhibit biofilm formation by aggregatibacter actinomycetemcomitans, klebsiella pneumoniae, staphylococcus aureus, s. epidermidis, candida albicans and k. kingae. the extracts evidently inhibited biofilm formation by modifying the physicochemical properties of the cell surface, the biofilm matrix, and the substrate. chemical and biochemical analyses indicated that the biofilm inhibition activity in the k. kingae extrac ... | 2011 | 21602333 |
| the utility of matrix assisted laser desorption/ionization time-of-flight mass spectrometry (maldi-tof ms) following introduction for routine laboratory bacterial identification. | maldi-tof ms was evaluated prospectively in a diagnostic laboratory. 927 organisms were tested in triplicate; 2351/2781 (85%) species and 2681/2781 (96%) genus identifications were correct. known issues such as the misidentification of α-haemolytic streptococci as streptococcus pneumonia were easily corrected. identifications cost $aud0.45 per isolate and were available in minutes. maldi-tof ms is rapid, accurate and inexpensive. | 2011 | 21632894 |
| antibiotic treatment for acute haematogenous osteomyelitis of childhood: moving towards shorter courses and oral administration. | acute haematogenous osteomyelitis (ahom) of childhood usually affects the long bones of the lower limbs. although almost any agent may cause ahom, staphylococcus aureus is the most common bacterium, followed by streptococcus pneumoniae and, in some countries, salmonella spp. and kingella kingae. magnetic resonance imaging (mri) has improved the diagnostic accuracy of traditional radiography and scintigraphy. except for the pre-treatment diagnostic sample from bone before the institution of antib ... | 2011 | 21640559 |
| [antibiotic therapy of bone and joint infections in children: recent changes]. | management of bone and joint infections in children associates early appropriate antibiotic therapy against staphylococcus aureus and kingella kingae and, if necessary, surgical drainage of abscess or septic arthritis. in 2007, the paediatric infectious diseases group of the french society of paediatrics (gpip) proposed guidelines for antibiotherapy in acute and non-complicated cases, with an intravenous therapy during 4 to 7days followed by oral therapy during 3weeks. | 2011 | 21733665 |
| advances in the diagnosis and management of pediatric osteomyelitis. | acute hematogenous osteomyelitis is a common disease that affects previously healthy children of all age groups. despite its importance, there are limited data in the current literature to guide many aspects of the diagnosis and therapy of this infection. over the last decade, our understanding of the etiology of this infection has changed, with increased recognition of kingella kingae and the dramatic increase in community-associated methicillin-resistant staphylococcus aureus (ca-mrsa) infecti ... | 2011 | 21789499 |
| abiotrophia defectiva knee prosthesis infection: a case report. | abstract: | 2011 | 21896178 |
| can early mri distinguish between kingella kingae and gram-positive cocci in osteoarticular infections in young children? | background: k. kingae is a common causative organism in acute osteoarticular infections (oais) in children under 4 years of age. differentiation between k. kingae and gram-positive cocci (gpc) is of great interest therapeutically. objective: our aim was to identify early distinguishing mri features of oais. materials and methods: thirty-one children younger than 4 years of age with oai underwent mri at presentation. of these, 21 were caused by k. kingae and ten by gpc. bone and soft tissue react ... | 2011 | 21909715 |
| Kingella kingae endocarditis: A rare case of mitral valve perforation. | Kingella kingae, a HACEK (Haemophilus parainfluenzae, Aggregatibacter actinomycetemcomitans, Aggregatibacter aphrophilus, Cardiobacterium hominis, Eikenella corrodens, Kingella kingae) organism, is a common resident of the upper airway in children; it has been associated with endocarditis in children with pre-existing heart conditions. This case report describes K. kingae endocarditis leading to valvular damage in a previously healthy 18-month-old child. Our patient developed a K. kingae bactere ... | 2011 | 21976892 |
| Direct Detection of Staphylococcus Osteoarticular Infections by Use of Xpert MRSA/SA SSTI Real-Time PCR. | We evaluated the Xpert MRSA/SA SSTI real-time PCR assay (Cepheid, Sunnyvale, CA) directly on perioperative bone and joint samples. The sensitivity and specificity for detection of methicillin-susceptible Staphylococcus aureus (MSSA), methicillin-resistant Staphylococcus aureus (MRSA), and methicillin-resistant coagulase-negative Staphylococcus were, respectively, 100% and 98.3%, 100% and 100%, and 100% and 95.3%. The median total test turnaround time was 72 min for PCR versus 79 h for culture. U ... | 2011 | 21998420 |
| [espondilodiskitis caused by kingella kingae in children: a case report]. | during the last years, spondilodiskitis due to kingella kingae has been a new target of interest, since it is the second agent that causes non tuberculous espondilodiskitis in children, after staphylococcus aureus. its clinical presentation is very inespecific, sometimes with fever, abdominal or lumbar disconfort, nocturnal pain, altered walking and sedestation. images, culture methods and pcr (polymerase chain reaction) can be helpful for diagnosis, although in an important percent of the cases ... | 2011 | 22052404 |
| gangliosides block aggregatibacter actinomycetemcomitans leukotoxin (ltxa)-mediated hemolysis. | aggregatibacter actinomycetemcomitans is an oral pathogen and etiologic agent of localized aggressive periodontitis. the bacterium is also a cardiovascular pathogen causing infective endocarditis. a. actinomycetemcomitans produces leukotoxin (ltxa), an important virulence factor that targets white blood cells (wbcs) and plays a role in immune evasion during disease. the functional receptor for ltxa on wbcs is leukocyte function antigen-1 (lfa-1), a ß-2 integrin that is modified with n-linked car ... | 2010 | 22069577 |
| Predictive score to discriminate Kingella kingae from Staphylococcus aureus arthritis in France. | 2011 | 22105423 | |
| Simplifying the treatment of acute bacterial bone and joint infections in children. | The treatment of acute hematogenous bone and joint infections of children - osteomyelitis (OM), septic arthritis (SA) and OM-SA combination (OM+SA) - has simplified over the past years. The old approach included months-long antibiotic treatment, started intravenously for at least a week, followed by oral completion of the course. Recent prospective randomized trials show that most cases heal with a total course of 3 weeks (OM, OM+SA) or 2 weeks (SA) of an appropriate antibiotic, provided the cli ... | 2011 | 22114963 |
| Kingella kingae Infections in Children: An Update. | 2011 | 22125036 | |
| high frequency of tropheryma whipplei in culture-negative endocarditis. | "classical" whipple's disease (cwd) is caused by tropheryma whipplei and is characterized by arthropathy, weight loss, and diarrhea. t. whipplei infectious endocarditis (twie) is rarely reported, either in the context of cwd or as isolated twie without signs of systemic infection. the frequency of twie is unknown, and systematic studies are lacking. here, we performed an observational cohort study on the incidence of t. whipplei infection in explanted heart valves in two german university center ... | 2011 | 22135251 |
| Epidemiology of Invasive Kingella kingae Infections in Two Distinct Pediatric Populations Cohabiting in One Geographic Area. | The annual incidence of invasive Kingella kingae younger than 4 years of age was significantly higher in westernized Jews than in indigent Bedouins living side-by-side in southern Israel (12.21/100,000 and 5.83/100,000, respectively, (P<0.05). One K. kingae clone was over-represented among isolates from Jewish children, suggesting that differences in bacterial pathogenicity may contribute to the morbidity excess detected in this population group. | 2011 | 22146739 |
| kingella kingae endocardial abscess and cerebral infarction in a previously well immunocompetent child. | kingella kingae is an emerging paediatric pathogen that most commonly is associated with relatively benign osteoarticular disease in children. this report concerns a 1-year-old child with kingella kingae endocarditis and perivalvular abscess complicated by septic cerebral emboli and osteomyelitis leading to long-term neurological sequelae, highlighting the capacity of this organism to cause severe invasive disease in children. | 2009 | 22190987 |
| pseudomonas aeruginosa pily1 binds integrin in an rgd- and calcium-dependent manner. | pily1 is a type iv pilus (tfp)-associated protein from the opportunistic pathogen pseudomonas aeruginosa that shares functional similarity with related proteins in infectious neisseria and kingella species. previous data have shown that pily1 acts as a calcium-dependent pilus biogenesis factor necessary for twitching motility with a specific calcium binding site located at amino acids 850-859 in the 1,163 residue protein. in addition to motility, pily1 is also thought to play an important role i ... | 2011 | 22242136 |
| antibiotic susceptibility of kingella kingae isolates from children with skeletal system infections. | 2012 | 22252209 | |
| dual priming oligonucleotides for broad-range amplification of the bacterial 16s rrna gene directly from human clinical specimens. | broad-range amplification and sequencing of the bacterial 16s rrna gene directly from clinical specimens are offered as a diagnostic service in many laboratories. one major pitfall is primer cross-reactivity with human dna which will result in mixed chromatograms. mixed chromatograms will complicate subsequent sequence analysis and impede identification. in sybr green real-time pcr assays, it can also affect crossing threshold values and consequently the status of a specimen as positive or negat ... | 2012 | 22278843 |
| human immunodeficiency virus & cardiovascular risk. | highly active antiretroviral therapy (haart) significantly changed the prevalence of the cardiovascular manifestations of human immunodeficiency virus (hiv)/aids. in developed countries, a 30 per cent reduction in the prevalence of cardiomyopathy and pericardial effusion was observed, possibly related to a reduction of opportunistic infections and myocarditis. in developing countries, however, where the availability of haart is limited, and the pathogenic impact of nutritional factors is signifi ... | 2011 | 22310821 |
| an assessment of the current diagnostic criteria for infective endocarditis. | a firm diagnosis of infective endocarditis is difficult to establish. clinicians often face a decision-making dilemma about whether to treat a patient presenting with some features that suggest endocarditis. this article summarizes both the previous and the recently proposed criteria for the diagnosis of infective endocarditis, and reviews the efficacy of the new criteria. the authors also discuss the experience at the toronto hospital, toronto, ontario, with this disease, paying specific attent ... | 1998 | 22346546 |
| pacemaker endocarditis due to haemophilus parainfluenza: case report and literature review. | we report a case of pacemaker infective endocarditis in a 14 month old girl, caused by haemophilus parainfluenzae. there are no other cases in children reported in the literature. we discuss the issues surrounding the case and the evidence which influenced our management. | 2007 | 22368676 |
| current concepts in laboratory testing to guide antimicrobial therapy. | antimicrobial susceptibility testing (ast) is indicated for pathogens contributing to an infectious process that warrants antimicrobial therapy if susceptibility to antimicrobials cannot be predicted reliably based on knowledge of their identity. such tests are most frequently used when the etiologic agents are members of species capable of demonstrating resistance to commonly prescribed antibiotics. some organisms have predictable susceptibility to antimicrobial agents (ie, streptococcus pyogen ... | 2012 | 22386185 |
| isolation of kingella kingae in the oropharynx during k. kingae arthritis in children. | kingella kingae arthritis in children is now mainly diagnosed by pcr, which has surpassed conventional culture of joint fluid. as oropharynx colonization is the first step of kingella kingae invasion, we prospectively investigated the possibility of cultivating it from throat swabs, in children hospitalized for k. kingae arthritis. throat culture was 5.6-fold more sensitive than joint fluid cultures in isolating k. kingae (66.7% vs. 11.9% respectively, p <0.001) and may be used to perform antibi ... | 2012 | 22390653 |
| cardiac device-related endocarditis: epidemiology, pathogenesis, diagnosis and treatment - a review. | cardiac device-related endocarditis (cde) is a phenomenon for which incidence is on the rise; it presents difficult management problems to the clinician. on one hand, there is the patient who needs the implanted device, and the potential morbidity and mortality associated with its removal. on the other hand, there is the problem of a persistent infection - usually acquired during insertion of an electrical device - that is resistant to many antibiotics, has a high recurrence rate, and necessitat ... | 2009 | 22477546 |
| haematogenous acute and subacute paediatric osteomyelitis: a systematic review of the literature. | a delay in the diagnosis of paediatric acute and subacute haematogenous osteomyelitis can lead to potentially devastating morbidity. there are no definitive guidelines for diagnosis, and recommendations in the literature are generally based on expert opinions, case series and cohort studies. all articles in the english literature on paediatric osteomyelitis were searched using medline, cinahl, embase, google scholar, the cochrane library and reference lists. a total of 1854 papers were identifie ... | 2012 | 22529075 |
| small risk of osteoarticular infections in children with asymptomatic oropharyngeal carriage of kingella kingae. | the aim of this study was to evaluate the absolute risk for children younger than 4 years of age with asymptomatic oropharyngeal carriage of kingella kingae to sustain an osteoarticular infection. the rate of k. kingae carriage in the oropharyngeal mucosa was 9% among healthy children, and the risk for an asymptomatic carrier to develop an osteoarticular infection due to k. kingae was estimated to be lower than 1%. | 2012 | 22572754 |
| genome sequence of kingella kingae septic arthritis isolate pykk081. | kingella kingae is a human oral bacterium that can cause infections of the skeletal system in children. the bacterium is also a cardiovascular pathogen causing infective endocarditis in children and adults. we report herein the draft genome sequence of septic arthritis k. kingae strain pykk081. | 2012 | 22582375 |
| bench-to-bedside review: rapid molecular diagnostics for bloodstream infection--a new frontier? | among critically ill patients, the diagnosis of bloodstream infection poses a major challenge. current standard bacterial identification based on blood culture platforms is intrinsically time-consuming and slow. the continuous evolvement of molecular techniques has the potential of providing a faster, more sensitive and direct identification of causative pathogens without prior need for cultivation. this may ultimately impact clinical decision-making and antimicrobial treatment. this review summ ... | 2012 | 22647543 |
| culture negative endocarditis: a diagnostic and therapeutic challenge! | infective endocarditis remains a diagnostic and therapeutic challenge. the authors report an older male who presented with a lower extremities rash and signs of cardiac failure. echocardiography showed vegetations attached to the bio-prosthetic aortic valve, however, with negative blood cultures. further investigation revealed positive bartonella serologies. the patient was diagnosed with bartonella endocarditis affecting the prosthetic valve and started on appropriate antibiotics. valvular surg ... | 2011 | 22674101 |
| multilocus sequence typing and rtxa toxin gene sequencing analysis of kingella kingae isolates demonstrates genetic diversity and international clones. | kingella kingae, a normal component of the upper respiratory flora, is being increasingly recognized as an important invasive pathogen in young children. genetic diversity of this species has not been studied. | 2012 | 22693588 |
| antibiofilm polysaccharides. | bacterial extracellular polysaccharides have been shown to mediate many of the cell-to-cell and cell-to-surface interactions that are required for the formation, cohesion and stabilization of bacterial biofilms. however, recent studies have identified several bacterial polysaccharides that inhibit biofilm formation by a wide spectrum of bacteria and fungi both in vitro and in vivo. this review discusses the composition, modes of action and potential biological roles of antibiofilm polysaccharide ... | 2012 | 22730907 |
| antibiofilm polysaccharides. | bacterial extracellular polysaccharides have been shown to mediate many of the cell-to-cell and cell-to-surface interactions that are required for the formation, cohesion and stabilization of bacterial biofilms. however, recent studies have identified several bacterial polysaccharides that inhibit biofilm formation by a wide spectrum of bacteria and fungi both in vitro and in vivo. this review discusses the composition, modes of action and potential biological roles of antibiofilm polysaccharide ... | 2012 | 22730907 |
| septic arthritis caused by kingella kingae: a case report. | 2011 | 22737437 | |
| actinobacillus actinomycetemcomitans endocarditis in a 1.5 year old toddler. | the authors report a 1.5-year-old girl who developed actinobacillus actinomycetemcomitans (aa) endocarditis involving the pulmonic valve. she had a congenital cardiac abnormality, but no history of dental manipulation. the case illustrates an uncomplicated course with three unique features; the youngest reported infant with endocarditis caused by aa with vegetation on the pulmonic valve. she underwent a benign course with complete recovery. the authors report a 1.5-year-old girl who developed aa ... | 2010 | 22767569 |
| aortic homograft endocarditis caused by cardiobacterium hominis and complicated by agranulocytosis due to ceftriaxone. | the present report describes a very rare case of an aortic homograft valve endocarditis caused by cardiobacterium hominis. the case was complicated by an agranulocytosis after 3 weeks of antibiotic treatment induced by ceftriaxone. alternative oral treatment with ciprofloxacin and rifampicin was successful, no surgical intervention was needed and homograft function could be preserved. | 2010 | 22797477 |
| genotyping of invasive kingella kingae isolates reveals predominant clones and association with specific clinical syndromes. | despite the increasing recognition of kingella kingae as an important pathogen of early childhood, the relative frequency and invasiveness of different strains of the organism has not been investigated. a study was conducted to determine the association of k. kingae genotypes with specific clinical syndromes and the temporal and geographic distribution of invasive clones. | 2012 | 22806593 |
| actin cross-linking domain of aeromonas hydrophila repeat in toxin a (rtxa) induces host cell rounding and apoptosis. | the repeat in toxin (rtx) of an environmental isolate atcc 7966 of aeromonas hydrophila consists of six genes (rtxachbde) organized in an operon similar to the gene organization found for the rtx of the vibrio species. the first gene in this operon (rtxa) encodes an exotoxin in vibrios, while other genes code for proteins needed for proper activation of rtxa and in secretion of this toxin from vibrio cholerae. however, the rtxa of atcc 7966, as well as from the clinical isolate ssu of a. hydroph ... | 2012 | 22814176 |
| infective endocarditis complicated by aortic graft infection and osteomyelitis: case report and review of literature. | primary aortic graft infection early after aortic graft insertion is well described in the literature. here, we present a unique case of late aortic graft infection 5 years after insertion secondary to mitral valve endocarditis, resulting from cellulitis in a patient with severe venous varicosities. a 63-year-old male presented for severe low back pain, constipation, and low-grade fever. an abdominal computed tomography scan with oral and intravenous contrast showed a normal spine and urinary tr ... | 2012 | 22866008 |
| childhood osteomyelitis: imaging characteristics. | the purpose of this review is to illustrate the imaging findings of childhood osteomyelitis. the diagnosis of childhood osteomyelitis can be challenging. clinical presentation and laboratory results can differ and are relatively unreliable. to date, its role in the assessment of treatment efficacy is not yet clear. | 2012 | 22875760 |
| reduced set of virulence genes allows high accuracy prediction of bacterial pathogenicity in humans. | although there have been great advances in understanding bacterial pathogenesis, there is still a lack of integrative information about what makes a bacterium a human pathogen. the advent of high-throughput sequencing technologies has dramatically increased the amount of completed bacterial genomes, for both known human pathogenic and non-pathogenic strains; this information is now available to investigate genetic features that determine pathogenic phenotypes in bacteria. in this work we determi ... | 2012 | 22916122 |
| the epidemiology of pediatric bone and joint infections in cambodia, 2007-11. | there are limited data on osteoarticular infections from resource-limited settings in asia. a retrospective study of patients presenting to the angkor hospital for children, cambodia, january 2007-july 2011, identified 81 cases (28% monoarticular septic arthritis, 51% single-limb osteomyelitis and 15% multisite infections). the incidence was 13.8/100 000 hospital attendances. the median age was 7.3 years, with a male/female ratio of 1.9:1; 35% presented within 5 days of symptom onset (median 7 d ... | 2012 | 22977206 |
| the epidemiology of pediatric bone and joint infections in cambodia, 2007-11. | there are limited data on osteoarticular infections from resource-limited settings in asia. a retrospective study of patients presenting to the angkor hospital for children, cambodia, january 2007-july 2011, identified 81 cases (28% monoarticular septic arthritis, 51% single-limb osteomyelitis and 15% multisite infections). the incidence was 13.8/100 000 hospital attendances. the median age was 7.3 years, with a male/female ratio of 1.9:1; 35% presented within 5 days of symptom onset (median 7 d ... | 2012 | 22977206 |
| risk for invasive kingella kingae infections and day-care facility attendance. | 2012 | 22982952 | |
| prevalence of pharyngeal carriage of kingella kingae in young children and risk factors for colonization. | the carriage rate of kingella kingae among jewish and bedouin children living in southern israel paralleled the age-related incidence of invasive infections. age 6-29 months was a significant risk factor for k. kingae colonization in both ethnic groups; day-care attendance was a risk factor in jewish children, whereas living in a shanty town was negatively associated with carriage in bedouins. | 2013 | 23014360 |
| osteomyelitis pubis caused by kingella kingae in an adult patient: report of the first case. | kingella kingae is the second most common pathogen causing paediatric arthritis and is described to be the causative bacteria in some paediatric osteomyelitis. its microbiological detection is particularly difficult due to its slow growing. to our best knowledge this is the first case description of osteomyelitis pubis caused by this microorganism. | 2012 | 23031309 |
| genomic comparison of kingella kingae strains. | kingella kingae is a betaproteobacterium from the order neisseriales, and it is an agent of invasive infections in children. we sequenced the genome from the septic arthritis strain 11220434. it is composed of a 1,990,794-bp chromosome but no plasmid, and it contains 2,042 protein-coding genes and 52 rna genes, including 3 rrna genes. | 2012 | 23045489 |
| modulation of kingella kingae adherence to human epithelial cells by type iv pili, capsule, and a novel trimeric autotransporter. | kingella kingae is an emerging bacterial pathogen that is being recognized increasingly as an important etiology of septic arthritis, osteomyelitis, and bacteremia, especially in young children. colonization of the posterior pharynx is a key step in the pathogenesis of k. kingae disease. previous work established that type iv pili are necessary for k. kingae adherence to the respiratory epithelium. in this study, we set out to identify additional factors that influence k. kingae interactions wit ... | 2012 | 23093386 |
| defining falciparum-malaria-attributable severe febrile illness in moderate-to-high transmission settings on the basis of plasma pfhrp2 concentration. | in malaria-endemic settings, asymptomatic parasitemia complicates the diagnosis of malaria. histidine-rich protein 2 (hrp2) is produced by plasmodium falciparum, and its plasma concentration reflects the total body parasite burden. we aimed to define the malaria-attributable fraction of severe febrile illness, using the distributions of plasma p. falciparum hrp2 (pfhrp2) concentrations from parasitemic children with different clinical presentations. | 2012 | 23136222 |
| defining falciparum-malaria-attributable severe febrile illness in moderate-to-high transmission settings on the basis of plasma pfhrp2 concentration. | in malaria-endemic settings, asymptomatic parasitemia complicates the diagnosis of malaria. histidine-rich protein 2 (hrp2) is produced by plasmodium falciparum, and its plasma concentration reflects the total body parasite burden. we aimed to define the malaria-attributable fraction of severe febrile illness, using the distributions of plasma p. falciparum hrp2 (pfhrp2) concentrations from parasitemic children with different clinical presentations. | 2012 | 23136222 |
| pleuritis due to brevundimonas diminuta in a previously healthy man. | brevundimonas diminuta is rarely associated with invasive infections. we report the case of a previously healthy young man with pleural effusion, in which b. diminuta was recovered but incorrectly identified as kingella kingae when it was freshly isolated. consequently, the misidentification resulted in initial treatment failure. the correct identification was achieved through further incubation, sequencing of the 16s rrna gene and ms. | 2013 | 23180480 |
| type iv pilin proteins: versatile molecular modules. | type iv pili (t4p) are multifunctional protein fibers produced on the surfaces of a wide variety of bacteria and archaea. the major subunit of t4p is the type iv pilin, and structurally related proteins are found as components of the type ii secretion (t2s) system, where they are called pseudopilins; of dna uptake/competence systems in both gram-negative and gram-positive species; and of flagella, pili, and sugar-binding systems in the archaea. this broad distribution of a single protein family ... | 2012 | 23204365 |
| [kingella kingae pediatric septic arthritis]. | kingella kingae is a bacterium that colonizes the upper respiratory tract. despite its low pathogenicity in this location, previous respiratory pathological processes may favor its systemic spread causing bone and joint infections, mainly in children under five years. it can be considered an emerging pathogen in osteoarticular infection in pediatric patients. we report the case of a two-year-old girl with hips pain and limitation of both abduction and extension, and fever. radiography and ultras ... | 2012 | 23224317 |
| a toddler who refused to stand or walk: lumbar spondylodiscitis. | a young mother presented her 19-month-old boy to the general practitioner (gp) with a 24 h history of reluctance to stand or walk and a slightly raised temperature. the gp arranged an assessment by the paediatrician, who organised an ultrasound of the hips which was normal. approximately 1 week later the patient became constipated as well, was seen again by another gp but no cause was found. another week later mother consulted the initial gp again as the boy had not shown any signs ... | 2012 | 23230243 |
| calcium binding properties of the kingella kingae pilc1 and pilc2 proteins have differential effects on type iv pilus-mediated adherence and twitching motility. | kingella kingae is an emerging bacterial pathogen that is being recognized increasingly as an important etiology of septic arthritis, osteomyelitis, and bacteremia, especially in young children. the pathogenesis of k. kingae disease begins with bacterial adherence to respiratory epithelium, which is dependent on type iv pili and is influenced by two pilc-like proteins called pilc1 and pilc2. production of either pilc1 or pilc2 is necessary for k. kingae piliation and bacterial adherence. in this ... | 2013 | 23243304 |
| detection of kingella kingae osteoarticular infections in children by oropharyngeal swab pcr. | the purpose of this study was to investigate if oropharyngeal swab polymerase chain reaction (pcr) could predict osteoarticular infection (oai) due to kingella kingae in young children. | 2013 | 23248230 |
| comparing the oropharyngeal colonization density of kingella kingae between asymptomatic carriers and children with invasive osteoarticular infections. | colonization of the oropharynx by kingella kingae is currently considered to be a prerequisite for later development of invasive infections. however, the oropharyngeal k. kingae dna bacterial load in children with osteoarticular infections caused by this microorganism is not different than that of asymptomatic carriers. | 2013 | 23271444 |
| investigation of an outbreak of osteoarticular infections caused by kingella kingae in a childcare center using molecular techniques. | we describe an outbreak of 5 osteoarticular infections among 24 daycare center attendees. polymerase chain reaction revealed kingella kingae in the joint fluid of 1 child and in 85% of throat samples from healthy contacts. multilocus sequence typing performed on the joint fluid and carriage isolates identified an unique sequence type. rifampin failed to eradicate k. kingae carriage. | 2013 | 23348810 |
| construction of new cloning, lacz reporter and scarless-markerless suicide vectors for genetic studies in aggregatibacter actinomycetemcomitans. | to elucidate the putative function of a gene, effective tools are required for genetic characterization that facilitate its inactivation, deletion or modification on the bacterial chromosome. in the present study, the nucleotide sequence of the escherichia coli/aggregatibacter actinomycetemcomitans shuttle vector pygk was determined, allowing us to redesign and construct a new shuttle cloning vector, pjt4, and promoterless lacz transcriptional/translational fusion plasmids, pjt3 and pjt5. plasmi ... | 2013 | 23353051 |
| infective endocarditis developing serious multiple complications. | a 20-year-old female patient with no history of heart disease presented to the hospital with high fever, cough and sputum. on the third day of hospitalisation, left facial paralysis developed and cranial revealed an infarct in the right frontoparietotemporal lobe. transthoracic echocardiography revealed 1.5 × 2 cm-sized vegetation on the mitral valve and severe mitral regurgitation. on the 11th day of hospitalisation, multiple emboli were shown to be present in the left iliac artery. since the p ... | 2013 | 23362066 |
| 30 years of study of kingella kingae: post tenebras, lux. | kingella kingae is a gram-negative bacterium that is today recognized as the major cause of joint and bone infections in young children. this microorganism is a member of the normal flora of the oropharynx, and the carriage rate among children under 4 years of age is approximately 10%. k. kingae is transmitted from child to child through close personal contact. key virulence factors of k. kingae include expression of type iv pili, knh-mediated adhesive activity and production of a potent rtx tox ... | 2013 | 23374128 |
| antimicrobial susceptibilities of clinical isolates of hacek organisms. | the "hacek" organisms are a group of fastidious gram-negative bacteria that cause a variety of infections, including infective endocarditis. antimicrobial susceptibility testing is not universally available, and therapy for these infections is often empirical. we report the antimicrobial susceptibilities of 70 clinical hacek isolates to 18 antimicrobials. all isolates were susceptible to ceftriaxone and levofloxacin, indicating that these agents remain appropriate empirical choices for the treat ... | 2013 | 23403420 |
| molecular mapping to species level of the tonsillar crypt microbiota associated with health and recurrent tonsillitis. | the human palatine tonsils, which belong to the central antigen handling sites of the mucosal immune system, are frequently affected by acute and recurrent infections. this study compared the microbiota of the tonsillar crypts in children and adults affected by recurrent tonsillitis with that of healthy adults and children with tonsillar hyperplasia. an in-depth 16s rrna gene based pyrosequencing approach combined with a novel strategy that included phylogenetic analysis and detection of species ... | 2013 | 23437130 |
| treatment of acute septic arthritis. | acute septic arthritis is a rare, but potentially devastating disease. the treatment is initiated intravenously, but can be safely switched to oral after 2-4 days providing large doses of a well-absorbing antibiotic and, for time-dependent antibiotics, 4 times-a-day administration are used. empiric treatment should always cover staphylococcus aureus and common respiratory pathogens, whereas kingella kingae and salmonella are important only regionally. studies conducted by our group have shown th ... | 2013 | 23439494 |
| kingella kingae keratitis. | we present the first two cases of kingella kingae keratitis in adults. this species is a rare isolate from ophthalmic samples for which final identification was obtained with matrix-assisted laser desorption ionization-time of flight mass spectrometry. one of the patients recovered uneventfully with topical therapy. results from the second patient cannot be confirmed as he was lost to follow-up. | 2013 | 23447636 |
| beta-lactamase production by kingella kingae in israel is clonal and common in carriage organisms but rare among invasive strains. | the purpose of this study was to investigate the prevalence of β-lactamase and the genomic clonality of a large collection of kingella kingae isolates from israeli patients with a variety of invasive infections and asymptomatic pharyngeal carriers. β-lactamase production was studied by the nitrocefin method and the minimum inhibitory concentrations (mics) of penicillin and amoxicillin-clavulanate were determined by the epsilon (etest) method. the genotypic clonality of isolates was investigated ... | 2013 | 23479041 |
| septic arthritis caused by noncapsulated haemophilus influenzae. | since the introduction of type b haemophilus influenzae vaccination, noncapsulated h. influenzae has become responsible for most cases of invasive h. influenzae diseases. in our two cases of septic arthritis, we isolated strains with β-lactamase-positive amoxicillin-clavulanate resistance (blpacr). thus, the increasing prevalence of blpacr should be taken into account when empirical therapy is chosen for septic arthritis. | 2013 | 23515545 |
| prosthetic valve endocarditis and bloodstream infection due to mycobacterium chimaera. | prosthetic valve endocarditis (pve) due to fast-growing nontuberculous mycobacteria (ntm) has been reported anecdotally. reports of pve with slowly growing ntm, however, are lacking. we present here one case of pve and one case of bloodstream infection caused by mycobacterium chimaera. randomly amplified polymorphic dna (rapd)-pcr indicated a relatedness of the two m. chimaera strains. both patients had heart surgery 2 years apart from each other. a nosocomial link was not detected. | 2013 | 23536407 |
| a critical role for the ccca gene product, cytochrome c2, in diverting electrons from aerobic respiration to denitrification in neisseria gonorrhoeae. | neisseria gonorrhoeae is a microaerophile that, when oxygen availability is limited, supplements aerobic respiration with a truncated denitrification pathway, nitrite reduction to nitrous oxide. we demonstrate that the ccca gene of neisseria gonorrhoeae strain f62 (accession number ng0292) is expressed, but the product, cytochrome c2, accumulates to only low levels. nevertheless, a ccca mutant reduced nitrite at about half the rate of the parent strain. we previously reported that cytochromes c4 ... | 2013 | 23543713 |
| rapid discrimination of haemophilus influenzae, h. parainfluenzae, and h. haemolyticus by fluorescence in situ hybridization (fish) and two matrix-assisted laser-desorption-ionization time-of-flight mass spectrometry (maldi-tof-ms) platforms. | due to considerable differences in pathogenicity, haemophilus influenzae, h. parainfluenzae and h. haemolyticus have to be reliably discriminated in routine diagnostics. retrospective analyses suggest frequent misidentifications of commensal h. haemolyticus as h. influenzae. in a multi-center approach, we assessed the suitability of fluorescence in situ hybridization (fish) and matrix-assisted laser-desorption-ionization time-of-flight mass-spectrometry (maldi-tof-ms) for the identification of h ... | 2013 | 23646201 |
| a case of kingella kingae endocarditis complicated by native mitral valve rupture. | we report a case of kingella kingae endocarditis in a patient with a history of recent respiratory tract infection and dental extraction. this case is remarkable for embolic and vasculitic phenomena in association with a large valve vegetation and valve perforation. kingella kingae is an organism known to cause endocarditis, however early major complications are uncommon. our case of kingella endocarditis behaved in a virulent fashion necessitating a combined approach of intravenous antibiotic t ... | 2013 | 23671460 |
| hacek-induced endocarditis. | a 61-year-old patient with diabetes had a bio-prosthetic aortic valve replacement 3 years before admission. he complained of lethargy, night sweats, decreased appetite and erratic blood glucose with no weight loss. he had splinter haemorrhage and a systolic ejection murmur at the aortic area. chest and abdominal examination revealed no abnormality. the erythrocyte sedimentation rate and c reactive protein were raised. he had several sets of blood cultures and he was started on empirical vancomyc ... | 2013 | 23682079 |
| antibiofilm activity of actinobacillus pleuropneumoniae serotype 5 capsular polysaccharide. | cell-free extracts isolated from colony biofilms of actinobacillus pleuropneumoniae serotype 5 were found to inhibit biofilm formation by staphylococcus aureus, s. epidermidis and aggregatibacter actinomycetemcomitans, but not by a. pleuropneumoniae serotype 5 itself, in a 96-well microtiter plate assay. physical and chemical analyses indicated that the antibiofilm activity in the extract was due to high-molecular-weight polysaccharide. extracts isolated from a mutant strain deficient in the pro ... | 2013 | 23691104 |
| [a case of osteomyelitis due to kingella kingae]. | kingella species including k. kingae are non-motile coccobacilli or short straight rods, and their normal habitats appear to be the upper respiratory and oropharyngeal tracts of humans. in recent years, k. kingae strains have been increasingly recognized as common causes of invasive infections in children at the age of less than 4 years. in japan, however, invasive k. kingae infections including osteomyelitis have rarely been described. we incidentally encountered isolation of a k. kingae strain ... | 2013 | 23713331 |
| two atypical cases of kingella kingae invasive infection with concomitant human rhinovirus infection. | we describe two atypical cases of kingella kingae infection in children diagnosed by pcr, one case involving a soft tissue abscess and one case a femoral brodie abscess. both patients had concomitant human rhinovirus infection. k. kingae strains, isolated from an oropharyngeal swab, were characterized by multilocus sequence typing and rtxa sequencing. | 2013 | 23784119 |
| characterization of tem-1 β-lactamase producing kingella kingae clinical isolates. | kingella kingae is a human pathogen that causes pediatric osteoarticular infections and infective endocarditis in children and adults. the bacterium is usually susceptible to β-lactam antibiotics, although β-lactam resistance has been reported in rare isolates. this study was conducted to identify β-lactam resistant strains and characterize the resistance mechanism. screening of a set of 90 k. kingae clinical isolates obtained from different geographic locations revealed high level resistance to ... | 2013 | 23796935 |
| bacteria in the nose of young adults during wellness and rhinovirus colds: detection by culture and microarray methods in 100 nasal lavage specimens. | patients with viral respiratory infections/viral rhinitis/common colds are often treated with antibiotic; however, there is little information on whether or how bacterial microbiota in the nose and nasopharynx might influence the course of viral illnesses. | 2013 | 23801660 |
| pyogenic flexor tenosynovitis of the finger due to kingella kingae. | 2013 | 23838734 | |
| oropharyngeal colonization density of kingella kingae. | 2013 | 23838786 | |
| a guide to utilization of the microbiology laboratory for diagnosis of infectious diseases: 2013 recommendations by the infectious diseases society of america (idsa) and the american society for microbiology (asm)(a). | the critical role of the microbiology laboratory in infectious disease diagnosis calls for a close, positive working relationship between the physician and the microbiologists who provide enormous value to the health care team. this document, developed by both laboratory and clinical experts, provides information on which tests are valuable and in which contexts, and on tests that add little or no value for diagnostic decisions. sections are divided into anatomic systems, including bloodstream i ... | 2013 | 23845951 |
| accurate identification of fastidious gram-negative rods: integration of both conventional phenotypic methods and 16s rrna gene analysis. | accurate identification of fastidious gram-negative rods (gnr) by conventional phenotypic characteristics is a challenge for diagnostic microbiology. the aim of this study was to evaluate the use of molecular methods, e.g., 16s rrna gene sequence analysis for identification of fastidious gnr in the clinical microbiology laboratory. | 2013 | 23855986 |
| unusually severe cases of kingella kingae osteoarticular infections in children. | with the development of molecular biology and specific polymerase chain reaction, kingella kingae has become the primary diagnosis of osteoarticular infections in young children. clinical features of these osteoarticular infections are typically mild, and outcome is almost always favorable. we report a series of unusually severe cases of k. kingae osteoarticular infections. | 2014 | 23856785 |
| acute endocarditis in intravenous drug users: a case report and literature review. | infective endocarditis (ie) is a notorious complication of intravenous drug use (idu). it typically affects the cardiac valves. among these, the tricuspid is the most common affected valve, although the mitral and/or aortic valves can also be involved. methicillin sensitive staphylococcus aureus (mssa) is the most common etiological microbial agent of ie in idu. once ie is diagnosed, antibiotic treatment should start immediately after blood cultures have been obtained. however, ie in this partic ... | 2012 | 23882351 |
| sigma factor rpon (σ54) regulates pile transcription in commensal neisseria elongata. | human-adapted neisseria includes two pathogens, neisseria gonorrhoeae and neisseria meningitidis, and at least 13 species of commensals that colonize many of the same niches as the pathogens. the type iv pilus plays an important role in the biology of pathogenic neisseria. in these species, sigma factor rpod (σ(70)), integration host factor, and repressors regf and crga regulate transcription of pile, the gene encoding the pilus structural subunit. the type iv pilus is also a strictly conserved ... | 2013 | 23899162 |
| prospective survey of acute osteoarticular infections in a french paediatric orthopedic surgery unit. | the epidemiology of acute paediatric osteoarticular infections (oai) has recently evolved, mainly due to the improvement of microbiological diagnosis. we conducted a prospective study to analyse the recent epidemiology and the clinical evolution of paediatric oai in order to validate the adequacy of our probabilistic first-line antibiotic treatment (intraveinous cefamandole + gentamicin). all children suspected of community acquired oai were included and followed-up for 3 years. the etiologic di ... | 2013 | 23957786 |
| clinical characteristics and thrombolytic outcomes of infective endocarditis-associated stroke. | our aim was to describe the clinical features of infective endocarditis (ie) in the acute stroke setting and outcomes following thrombolytic therapy. | 2012 | 23983869 |
| mutation of the conserved calcium-binding motif in neisseria gonorrhoeae pilc1 impacts adhesion but not piliation. | neisseria gonorrhoeae pilc1 is a member of the pilc family of type iv pilus-associated adhesins found in neisseria species and other type iv pilus-producing genera. previously, a calcium-binding domain was described in the c-terminal domains of pily1 of pseudomonas aeruginosa and in pilc1 and pilc2 of kingella kingae. genetic analysis of n. gonorrhoeae revealed a similar calcium-binding motif in pilc1. to evaluate the potential significance of this calcium-binding region in n. gonorrhoeae, we pr ... | 2013 | 24002068 |
| repeated serum alkaline phosphatase measurements in the treatment of childhood acute bone and joint infections with high doses of antibiotics. | 2013 | 24003432 | |
| cardiobacterium hominis-induced acute dacryocystitis and lacrimal abscess. | cardiobacterium hominis is a member of the hacek (haemophilus sp., actinobacillus actinomycetemcomitans, c. hominis, eikenella corrodens, and kingella kingae) group commonly associated with endocarditits and is normally present in the respiratory tract. we describe the first case of acute dacryocystitis with lacrimal abscess caused by c. hominis along with a brief review of the literature. the patient responded to oral and topical ciprofloxacin after incision and drainage and awaits dacryocystor ... | 2014 | 24008805 |
| functional genes to assess nitrogen cycling and aromatic hydrocarbon degradation: primers and processing matter. | targeting sequencing to genes involved in key environmental processes, i.e., ecofunctional genes, provides an opportunity to sample nature's gene guilds to greater depth and help link community structure to process-level outcomes. vastly different approaches have been implemented for sequence processing and, ultimately, for taxonomic placement of these gene reads. the overall quality of next generation sequence analysis of functional genes is dependent on multiple steps and assumptions of unknow ... | 2013 | 24062736 |
| incidence, risk factors and prediction of post-operative acute kidney injury following cardiac surgery for active infective endocarditis: an observational study. | cardiac surgery is frequently needed in patients with infective endocarditis (ie). acute kidney injury (aki) often complicates ie and is associated with poor outcomes. the purpose of the study was to determine the risk factors for post-operative aki in patients operated on for ie. | 2013 | 24093498 |