Publications

piliation and colonial morphology among laboratory strains of meningococci.	colonial morphology and piliation were studied on twelve strains from various serogroups of neisseria meningitidis. six different colony types (m1 to m6) were identified. most strains elaborated only an m1 colonial type, which is similar to gonococcus t4. several combinations of piliation and colonial morphology were observed: (i) colonial variation in which neither parent nor variant were piliated; (ii) colonial variation involving piliated and nonpiliated cells; (iii) dissociation of piliated ...	1978	99453
sympathetic joint effusion in septic arthritis.		1978	99527
iga protease production as a characteristic distinguishing pathogenic from harmless neisseriaceae.	iga proteases are extracellular enzymes of bacteria that have human immunoglobulin a of the iga1 subclass as their only known substrate. the identification of this enzyme in neisseria prompted us to determine whether iga protease production correlates with pathogenicity within this genus. multiple clinical isolates of neisseria gonorrhoeae, n. meningitidis and eight species of non-pathogenic neisseria that commonly colonize the normal human nasopharynx were examined for iga protease activity. al ...	1978	99655
[local, natural or artificial immunity, and its importance in the protection against meningococcal disease].		1978	99792
[meningococcus carriers among university hospital personnel].		1977	99793
[types of gonococci].		1978	99901
[electron-microscopic study of the effect of biseptol on the gonococcus in vitro and in vivo].		1978	99902
[study of biological properties of lyophilized gonococcal strains for the purpose of their use for vaccine production. 1].		1978	99903
[methacycline in the therapy of gonorrhea in men].		1978	99904
[antibiotic concentration in the serum of women with gonorrhea administered benzylpenicillin and bicillin-3].		1978	99905
[allergization of the body by neisseria meningitidis. i. the problem of the occurrence of n. meningitidis in cases of bronchial asthma of bacterial etiology (author's transl)].	the object of the authors work was the examination of nasopharyngeal swabs on the prevalence of neisseria meningitidis. a group of 222 patients suffering from asthma bronchiale of bacteriological etiology was under observation in order to be able to prepare an autovaccine. nasopharyngeal swabs were inoculated on special nutrient media. neisseria meningitidis was found in 22%, mainly from nasopharyngeal swabs, together with haemophilus sp. and streptococcus pneumoniae. in 59% of examined cases th ...	1978	99912
[fluorescent antibody technique in identification of neisseria gonorrhoeae-microcolonies grown on membrane filters (author's transl)].	filter membranes (nucleopore, no. n060, cpr 01300, pore size 0.6 mu) are inoculated with a pure suspension of n. gonorrhoeae or a urethral smear from patients suffering from gonococcal urethritis. the membranes are placed on chocolate agar and incubated for three hours. after fixation in formaldehyd, grown microcolonies of n. gonorrhoea are detected microscopically after incubation of the membranes with a fluorescein-isothiocyanate labeled gonococcal antiserum (difco, charge no. 605907) (fig. 3- ...	1978	99913
[antagonistic and bacteriocinogenic activity of meningococci].	antagonistic and bacteriocinogenic activity was studied in 169 strains of meningococci of various serological groups and with different localization in the human organism at the time of isolation. bacteriocinogenic activity was revealed in all the meningococcus strains (by delayed antagonism method), and, in addition, antagonistic activity was found in 100 strains. the inhibitory activity was the greatest in meningococci isolated from the cerebrospinal fluid. the data obtained suggest an importa ...	1978	99921
beta-lactamase producing haemophili and neisseriae.		1978	99937
cross-reactions between neisseria meningitidis and twenty-seven other bacterial species.	cross-reactions between antigens from n. meningitidis and antigens from 27 other bacterial species from clinical isolates representing 20 different genera were studied by quantitative immunoelectrophoretic methods. a water-soluble n. meningitidis antigen preparation and a corresponding pooled rabbit antiserum regularly presenting 48 immunoprecipitates were used as reference system. antigens from 16 other bacterial genera cross-reacted with one to four n. meningitidis antigens, whereas antigens f ...	1978	99974
[evaluation of the sanative action of rifampicin on the meningococcal carrier state].	the results of the epidemiological control experiment on the efficacy of rifampicin in sanation of meningococci carriers are presented. the preliminary study of rifampicin sensitivity of 41 freshly isolated nasopharyngeal meningococcal strains showed that the mic of the drug for 63 per cent of the isolates was 0.04--0.1 gamma/ml. sanation was performed for 2 days; 1.2 g of the drug was used during the treatment course. the results of examination of 91 meningococci carriers showed that 4 days aft ...	1978	100048
effect of a staphylococcin on neisseria gonorrhoeae.	phage group 2 staphylococcal strain ut0002 contains a large 56s virulence plasmid with genes that code for both exfoliative toxin and a specific staphylococcin termed bac r(1). four penicillinase-producing strains and three penicillin-susceptible strains of neisseria gonorrhoeae were killed by bac r(1). after 30 min of growth of the penicillin-resistant tr1 strain in 62.5 arbitrary units of bac r(1) per ml, loss of viability was approximately 90%, and, after 5 h, an approximately 99.99% loss of ...	1978	100049
identification of neisseria gonorrhoeae from primary cultures by a slide agglutination test.	hen antigonococcal lipopolysaccharide hen serum was used in a simple slide agglutination test for the identification of neisseria gonorrhoeae from primary isolates.	1978	100511
studies on the relationships between serum bactericidal activity and uncomplicated genital infections due to neisseria gonorrhoeae.	this report concerns the relationships between patients' uncomplicated genital infections due to neisseria gonorrhoeae and the bactericidal activity of their sera against their infecting organisms. forty isolates of n. gonorrhoeae and 91 serum samples were obtained from 23 patients with their first or repeated gonococcal infections. of 32 sera obtained at the time of infection, 14 (44%) were bactericidal for the infecting strains. of convalescent-phase sera from 27 infections, 12 (44%) were bact ...	1978	100561
familial deficiency of the seventh component of complement associated with recurrent bacteremic infections due to neisseria.	the serum of a 29-year old woman with a recent episode of disseminated gonococcal infection and a history of meningococcal meningitis and arthritis as a child was found to lack serum hemolytic complement activity. the seventh component of complement (c7) was not detected by functional or immunochemical assays, whereas other components were normal by hemolytic and immunochemical assessment. her fresh serum lacked complement-mediated bactericidal activity against neisseria gonorrhoeae, but the add ...	1978	100562
therapy of gonorrhea. comparison of trimethoprim-sulfamethoxazole and ampicillin.	eighty-nine men with gonococcal urethritis were randomly treated with trimethoprim-sulfamethoxazole, four tablets (trimethoprin, 320 mg, and sulfamethoxazole, 1,600 mg) twice daily for two days, or ampicillin, 3.5 g, plus probenecid, 1 g, in a single dose. forty-one (95.3%) of 43 patients who received trimethoprin-sulfamethosazole and 41 (97.6%) of 42 given ampicillin were cured. neither drug caused major side effects. all isolates of neisseria gonorrhoeae were susceptible in vitro to trimethopr ...	1978	100624
induction of dna synthesis in lymphocytes in vitro by various bacteria, with special reference to neisseria gonorrhoeae, in patients with uro-arthritis (reiter's disease).	antigen-induced lymphocyte proliferation was studied by quantitation of 14c-thymidine uptake in cell cultures. the induction of dna synthesis in vitro in lymphocytes from patients with uro-arthritis after stimulation by whole cells of virulent and avirulent n. gonorrhoeae, meningococci group b, and apathogenic neisseria (n. pharyngis) was compared with the dna synthesis in lymphocytes from healthy controls after stimulation with the same neisseria antigens. the difference between patients and th ...	1978	100875
a comparison of genital infections caused by chlamydia trachomatis and by neisseria gonorrhoeae.	patients with culture-proven infections with neisseria gonorrhoeae or chlamydia trachomatis, or both, seen at the olmsted county health department sexually transmitted disease clinic were evaluated for the presence of dysuria and exudate. eighty-four patients (21%) had neither symptom. n. gonorrhoeae infection alone was most frequent (47%), but c. trachomatis predominated in men. dysuria was most often detected in men infected with c. trachomatis, but exudate was most frequently found in men inf ...	1978	101076
[about proteinic impurities checking in a meningococcal vaccine. mannitol interference in the lowry protein determination (author's transl)].		1978	101124
patient variables associated with penicillin resistance in neisseria gonorrhoeae.	in an ongoing cooperative study of uncomplicated genital gonorrhea within the united states, 6,735 pretreatment gonococcal isolates were tested for their in vitro resistance to penicillin. by using a regression analysis technique, we examined 99 patient-associated variables which might be associated with penicillin resistance of the isolates. patients were most likely to have relatively resistant isolates if they were black, had used antimicrobial agents within 2 weeks of treatment for gonorrhea ...	1978	101129
in-vitro activity of antimicrobial agents against neisseria gonorrhoeae in brussels.	the minimum inhibitory concentrations (mics) of 18 antimicrobial agents against 104 strains of neisseria gonorrhoeae isolated in the brussels area between january and october 1976 have been measured. the mics for penicillin g, ampicillin, amoxycillin, carbenicillin, and cephalexin showed a bimodal distribution. the second modus strains of cephalexin (mic = 6.25 microgram/ml) were relatively resistant to penicillin g (mic greater than or equal to 0.08 microgram/ml). about 51% of all strains were ...	1978	101275
in-vitro antimicrobial susceptibility of neisseria gonorrhoeae in new zealand.	four hundred and forty-two isolates of neisseria gonorrhoeae were tested by an agar dilution method for their susceptibility to penicillin, ampicillin, tetracycline, cephaloridine, and spectinomycin. of these isolates, 295 were tested for their susceptibility to sulphamethoxazole and to trimethoprim by the same method, using oxoid diagnostic sensitivity test agar plus 7.5% laked horse blood instead of proteose no. 3 agar plus 1% isovitalex and 1% haemoglobin. one hundred (22.6%) of the isolates ...	1978	101276
which neisseria?		1978	101277
[experiences with a simple test system for the determination of beta-lactamase (penicillinase) in neisseria gonorrheae].		1978	101397
studies on gonococcus infection. xiv. cell wall protein differences among color/opacity colony variants of neisseria gonorrhoeae.	gonococci from colonies exhibiting optical opacity and dark coloration have surface proteins that are not visualized in isogenic transparent, light-colored colony forms. these "colony opacity-associated proteins" have apparent molecular weights varying from 24,000 to 30,000 by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate; their apparent molecular weights are independent of that for their major outer membrane protein. the opacity-associated proteins are more suscep ...	1978	101459
strain-dependent cytotoxic effects of endotoxin for mouse peritoneal macrophages.	the cytotoxic effects of bacterial lipopolysaccharides (lps) on mouse leukocytes have been examined in vivo and in vitro. intraperitoneal injection of lps into c57bl/6 mice greatly reduced the recovery of mononuclear cells; lps was cytotoxic for macrophages, but had a mitogenic effect on lymphocytes. similar effects of lps on peritoneal leukocytes were observed in vitro. when monolayers of adherent peritoneal cells were studied in vitro, cytotoxicity was also observed, suggesting that the effect ...	1978	101460
effects of iron and culture filtrates on killing of neisseria gonorrhoeae by normal human serum.	neisseria gonorrhoeae gc9, both colony types t2 and t4, were killed by normal human serum, although populations of colony type t4 were more susceptible. ferric ammonium citrate prevented the killing of populations of both t2 and t4 colony types. other iron compounds tested showed no protective effect, nor did ammonium citrate or the divalent cations magnesium or calcium. a filtrate from cultures of an n. gonorrhoeae strain grown in a liquid defined medium showed a similar protective effect in th ...	1978	101468
the activity of three oral cephalosporins against neisseria gonorrhoeae.		1978	101504
arginine biosynthesis in neisseria gonorrhoeae: enzymes catalyzing the formation of ornithine and citrulline.	many of the neisseria gonorrhoeae strains isolated from patients require arginine for growth in a defined medium. as a basis for genetic studies of these arg- strains, we examined two biosynthetic enzymes of arg+ (nonrequiring) gonococci. cell-free extracts contained (i) glutamate acetyltransferase, which catalyzes the formation of l-ornithine from alpha-n-acetyl-l-ornithine, and (ii) ornithine transcaramylase, which catalyzes the reaction between l-ornithine and carbamyl phosphate, yielding l-c ...	1978	101509
iron in neisseria meningitidis: minimum requirements, effects of limitation, and characteristics of uptake.	a simple defined medium (neisseria defined medium) was devised that does not require iron extraction to produce iron-limited growth of neisseria meningitidis (sdic). comparison of this medium to mueller-hinton broth and agar showed nearly identical growth rates and yields. the defined medium was used in batch cultures to determine the disappearance of iron from the medium and its uptake by cells. to avoid a number of problems inherent in batch culture, continuous culture, in which iron and disso ...	1978	101516
cell envelope alterations in antibiotic-sensitive and-resistant strains of neisseria gonorrhoeae.	the cell envelopes of antibiotic-resistant and -sensitive isogenic strains of neisseria gonorrhoeae were analyzed to determine whether acquisition of genetic loci for altered antibiotic sensitivity was accompanied by alterations in cell envelope composition. no differences in the composition of phospholipids and lipopolysaccharides were noted. acquisition of mtr-2, which results in low-level, nonspecific increased resistance to multiple antibiotics, dyes, and detergents, was accompanied by a sev ...	1978	101519
a simple manganous chloride and congo red disc method for differentiating neisseria gonorrhoeae from neisseria meningitidis.	manganous chloride and congo red incorporated into blotting paper discs have been used to differentiate gonococci from meningococci. the new technique is simple and reliable; the materials for the test are inexpensive. the method will increase the efficiency of distinguishing between the pathogenic neisseria in any clinical bacteriology laboratory and especially in those in the tropical areas.	1978	101555
[a case of bacterial endocarditis due to neisseria mucosa (author's transl)].		1978	101614
multiple antibiotic resistance in gonococci.		1978	101637
penicillin resistance in neisseria gonorrhoeae due to beta-lactamase production.	two isolates of neisseria gonorrhoeae from two patients who did not respond to repeated treatment with penicillin were found to contain an active beta-lactamase. this enzyme has not been previously found in other gonococci isolated in the united states. compared to other gonococci, these isolates had higher penicillin minimal inhibitory concentrations, and gave very small or no zones of inhibition in the disc agar diffusion test. the enzyme was demonstrated with three different rapid tests for b ...	1977	101741
penicillinase-producing gonococci.		1978	101754
[prostatitis inhibiting fertility. gonococci superseded by other microbes].		1978	101822
[unspecific prostato-urethritis. basis for the microbiological diagnosis].		1978	101830
acute joint infection with neisseria meningitidis: a case of mistaken identity.		1978	101903
[experimental contribution to the theme of pharyngeal gonorrhea].	experimental inoculations of the pharynx with gonococci were undertaken. in seven volunteers gonococci could be cultivated and identified from 16 hours to 8 weeks following infection. in three cases, it was tried to infect the female genital tract using positive pharyngeal smears. this was successful in one instance and the diagnosis was again confirmed by culture and sugar fermentation.	1978	102091
liberation of endotoxin during growth of neisseria meningitidis in a chemically-defined medium.	modified medium 199 is well suited to the growth of meningococci if rich growth is not necessary. the bacteria were very sensitive to changes in ph and needed a good buffer capacity of the medium. four strains of neisseria meningitidis were studied. after the stationary phase had been reached, the vital cell count decreased relatively slowly. however, the total cell count remained unchanged for at least 12 hours. the standardized e. coli endotoxin could be detected easily by the limulus lysate t ...	1978	102109
evaluation of the microcult-gc kit as a screening method for the detection of neisseria gonorrhoeae.	the microcult-gc kit was compared to the standard modified thayer-martin plate method for the isolation and presumptive identification of neisseria gonorrhoeae. two hundred and thirty specimens representing urethral, endocervical, rectal, and pharyngeal sites were inoculated to both media. if only genital and rectal cultures were compared, the overall agreement was 98 percent, with a false positive rate of 1/145 (0.7 percent) and a false negative rate of 2/145 (1.4 percent). however, in the phar ...	1978	102193
meningococcal infection in egypt: laboratory findings in meningitis patients and the prevalence of pharyngeal infection in patients and contacts.		1978	102212
meningococcal polysaccharide vaccines: recommendation of the public health service advisory committee on immunization practices.	meningococcal disease is endemic in the united states, and sulfonamide-sensitive serogroup b strains currently cause most cases. more than two thirds of all cases occur in persons less than 20 years old. three meningococcal polysaccharide vaccines are licensed for selective use in the united states: monovalent a, monovalent c, and bivalent a-c. this recommendation describes indications for their use, with particular attention to primary immunization, and use in pregnancy and in epidemic control.	1978	102231
comparison of the in vitro activity of bay k 4999 and piperacillin, two new antipseudomonal broad-spectrum penicillins, with other beta-lactam drugs.	bay k 4999 and piperacillin, two new substituted ampicillins, were compared with other beta-lactam antibiotics, including carbenicillin, azlocillin, mezlocillin, benzylpenicillin, ampicillin, and cefoxitin, against a wide range of gram-positive and -negative organisms. bay k 4999 and piperacillin were extremely active against pseudomonas aeruginosa (50% inhibited by 2 mug/ml), being about 16-fold more active than carbenicillin. bay k 4999 was the most active drug against escherichia coli (50% in ...	1978	102245
[radioimmunologic determination of antimeningococcal antibodies: technic and vaccination results].		1978	102435
heat-modifiable outer membrane proteins of neisseria meningitidis and their organization within the membrane.	neisseria meningitidis group b serotype 2 strain m986 contains two predominant outer membrane proteins, with apparent molecular weights of 41,000 (protein b) and 28,000 (protein e). heating of outer membrane vesicles at 56 degrees c for 20 min caused much of b** to disaggregate and denature into b (41,000 daltons). in contrast, protein e could be rapidly solubilized by sds at room temperature into its monomeric state (e*), but it was not converted to its final higher apparent molecular weight of ...	1978	102633
circulation of n. meningitidis in a child community.		1978	102690
changing concepts of pelvic inflammatory disease.		1978	102712
a simplified approach to cultures for gonorrhea.		1978	102730
the surface properties of neisseria gonorrhoeae: determinants of susceptibility to antibody complement killing.	monovalent rabbit antisera were prepared to highly purified gonococcal lipopolysaccharide (lps), to pili and to two major purified outer envelope proteins. all these antisera were free from significant specific igm antibody and were standardized to 4 microgram specific igg antibody per test, permitting accurate comparisons between the different gonococcal surface antigens as triggers of the complement-dependent bactericidal reaction. lps was the most effective antigen at inducing a bactericidal ...	1978	102732
the surface properties of neisseria gonorrhoeae: topographical distribution of the outer membrane protein antigens.	gonococci were labelled with 125i using the lactoperoxidase system. the amount of label incorporated was similar with all strains including those which appeared capsulated. electrophoresis on sodium dodecyl sulphate-polyacrylamide gels revealed that the major proteins labelled were those found in outer membrane preparations. comparison of variants of one strain showed that the major outer membrane protein (protein i) was always present and heavily labelled. the second major protein (protein ii) ...	1978	102733
laboratory-acquired gonococcal conjunctivitis.		1979	102807
[model of meningococcal sepsis in mice].	the authors studied a possibility of obtaining experimental meningococcus sepsis model on mice. the use of cyclophosphane, iron compounds, yolk medium produced no significant organism. when 4--5% mucine was injected intraperitoneally together with meningococcus culture mice died with sepsis phenomena. differences were revealed in the sensitivity of linear and mongrel mice to meningococcus infection--akr mice proved to be more sensitive. at the same time it was found that mongrel mice weighing fr ...	1978	103342
[participation of bacterial neuraminidases in the development of disease].		1978	103344
laboratory identification of pathogenic neisseria with special regard to atypical strains: an evaluation of sugar degradation, immunofluorescence and co-agglutination tests.	sugar degradation tests (sdt) were compared with immunofluorescence (ifl) and co-agglutination (coa) tests for the diagnosis of neisseria gonorrhoeae (gc) and neisseria meningitidis (mc). somewhat more than 5% of the gc strains and 8% of the mc strains were misinterpreted by sdt. on most occasions the disagreement between sdt and serological tests was due to the inability of the mc strains (less so for gc strains) to degrade sugars correctly. because of this, three out of 15 strains (20%) from p ...	1978	103365
effects of thiamphenicol and chloramphenicol in inhibiting neisseria gonorrhoeae isolates.	thiamphenicol was compared with penicillin, tetracycline, and chloramphenicol for its ability to inhibit 530 isolates of neisseria gonorrhoeae, including 13 penicillinase-producing isolates. thiamphenicol proved to be as active as chloramphenicol in inhibiting all of the isolates.	1978	103495
inhibition of beta-lactamase in neisseria gonorrhoeae by sodium clavulanate.	sodium clavulanate at subinhibitory concentrations affected the activity of penicillin g, ampicillin, or amoxicillin on beta-lactamase-positive strains of neisseria gonorrhoeae as demonstrated by marked reduction in the minimal inhibitory concentrations of the drugs for the organisms. the compound did not affect the activity of these penicillins on beta-lactamase-negative strains of n. gonorrhoeae. it also had no effect on the activity of cefoxitin against either beta-lactamase-negative or -posi ...	1978	103496
studies on gonococcus infection. xvi. purification of neisseria gonorrhoeae immunoglobulin a1 protease.	a protease which cleaves human immunoglobulin a1 (iga1) has been purified from broth cultures of neisseria gonorrhoeae. this iga1 protease is produced by pilated and nonpilated gonococci throughout their growth cycles. a combination of ammonium sulfate precipitation, column chromatography, and either isoelectric focusing or affinity chromatography was utilized to obtain an enzyme preparation that showed approximately 3,800-fold purification and exhibited two bands (65,000 and 70,000 daltons) by ...	1978	103829
gonococcal color and opacity variants: virulence for chicken embryos.	previous studies have noted that the prevalence of certain gonococcal colony types is influenced by the physiological state of the human host. consequently eight different gonococcal strains were passed on clear typing medium, and opaque (op) and transparent (tr) variants of heavily pilated (p++) bacteria were selected. p++op and p++tr were injected into 11-day-old chicken embryos, and the resulting mortality was determined. in every case the tr variants were more lethal to the embryos than were ...	1978	103830
[beta-lactamase producing gonococci in munich (author's transl)].	two more penicillinase-producing gonococci strains have been isolated in munich. the source of infection could not be traced in a 20 year old prostitute. a 45 year old patient contracted the infection during a stay in bangkok.	1978	103843
plaque assay for measuring serum bactericidal activity against gonococci.	a new semiquantitative plaque assay was developed to measure killing of gonococci by serum. results compare well with an established quantitative method, but the plaque assay is faster, simpler, and less expensive.	1978	103891
meningitis caused by neisseria meningitidis serogroup 135.	the first descriptions of meningitis in childhood caused by neisseria meningitidis serogroup 135 are presented. difficulties with identification of unusual serogroups of n. meningitidis are discussed.	1978	103892
chlamydia trachomatis cervicitis in gynecologic outpatients.	symptoms suggestive of a lower genital tract infection (lgti) are common complaints in women who consult gynecologists. sexually transmitted microorganisms, such as chlamydia trachomatis. neisseria gonorrhoeae, and trichomonas vaginalis, are responsible for a substantial proportion of lgti. this study was performed to establish the frequency of lgti caused by c trachomatis in women attending a gynecologic outpatient clinic. of 170 women with lgti, 32.9% harbored one or more of these organisms: c ...	1978	104212
[determination of colony types in gonococci].		1978	104458
[phagocytosis in urethral discharge before and during treatment of acute gonorrhea with methacycline].		1978	104468
[a rapid test for confirmation of "n. gonorrhoeae" (author's transl)].		1978	104667
contamination of metronidazole discs with penicillin.		1979	104759
method for studying the role of indigenous cervical flora in colonisation by neisseria gonorrhoeae.	a method for quantitating cervical flora has been evaluated statistically and used to study the bacterial flora of the cervix in 14 women sexually exposed to men with gonococcal urethritis. a comparison was made between those women who subsequently became colonised with neisseria gonorrhoeae and those who did not to determine whether either total microbial populations or the different species present could be related to colonisation by n. gonorrhoeae. two control groups of healthy women, one of ...	1978	104769
bulk growth of neisseria gonorrhoeae type 1 in a biphasic system.	a biphasic system for the bulk growth of neisseria gonorrhoeae is described. it appears to combine the convenience of liquid media with the growth qualities of solid media. by using the agar in dialysis tubes rather than as slabs the surface-to-volume ratio was improved and harvesting made easier. the system is also useful in isolating gonococci from blood and joint fluids in patients with disseminated infection.	1978	104770
isolation of chlamydia trachomatis from bartholin's ducts.	exudate from bartholin's ducts from 30 selected patients was investigated for chlamydia trachomatis and neisseria gonorrhoeae. n. gonorrhoeae was isolated from the duct exudate in 24 patients and c. trachomatis in nine. concurrent infection of the ducts was present in seven (29.2%); the remaining two patients were sexual contacts of men with non-specific urethritis. the duct exudate was mucopus in seven patients, cloudy mucus in one, and clear mucus in the other. although contamination of the vu ...	1978	104771
determination of meningococcal antibodies by microassay.	a modified microassay procedure, using triphenyltetrazolium chloride as a germination indicator, was compared with a macroassay method proposed by the world health organization for determining the level of antibodies before and after immunization with group a meningococcal polysaccharide vaccine. there was excellent agreement between the results obtained by the two methods. the vaccine used appeared to be safe and immunogenic.	1978	104795
antibiotic susceptibility testing of neisseria gonorrhoeae.		1979	104953
antibody responses of human infants to three doses of group a neisseria meningitidis polysaccharide vaccine administered at two, four, and six months of age.	infants were immunized with group a neisseria meningitidis polysaccharide vaccine at two, four, and six months of age. two lots of group a vaccine that differed in molecular weight were used; lot no. 1980a was of significantly larger molecular size than lot no. a-7. no significant adverse reactions occurred. the geometric mean concentration of serum antibody to group a n. meningitidis one month after the third dose of lot no. 1980a was 0.89 microgram/ml, nearly twice the concentration induced by ...	1978	105053
clinical and microbiological investigation of men with urethritis.	of 377 men attending clinics for the treatment of sexually transmitted disease, 104 had gonococcal urethritis, 72 had definite nongonococcal urethritis, 53 had possible nongonococcal urethritis, and 123 had no urethritis. a purulent urethral discharge was noted in 78% and 14% of patients with gonococcal urethritis and definite nongonococcal urethritis, respectively (p less than 0.001). in contrast, 4% and 64% of men with gonococcal urethritis and definite nongonococcal urethritis, respectively, ...	1978	105054
diagnosing gonococcal urethritis in males: experience in an oklahoma venereal disease clinic.		1979	105092
the identification of neisseria gonorrhoeae in liquid fermentation medium.		1979	105219
penicillinase-producing gonococci.		1978	105231
auxotypes and antibiotic susceptibility patterns of neisseria gonorrhoeae from disseminated and local infections.	the arginine-hypoxanthine-uracil auxotype has been linked with the propensity of gonococci to cause disseminated infections. gonococci recovered from 25 patients with disseminated gonococcal infections were compared with gonococci recovered from matched controls, patients with uncomplicated gonorrhea selected during the same month. minimal inhibitory concentrations of penicillin, tetracycline, erythromycin, and ampicillin, and the nutritional requirements (auxotypes) for proline alone, arginine ...	1978	105411
gonorrhea in the homosexual man: frequency of infection by culture site.	the aims of this study were determine the frequencies of infection with neisseria gonorrhoeae at various sites in homosexual men who were attending clinics for treatment of sexually transmitted diseases in central scotland and to appraise the diagnostic tests used. specimens for culture were taken from the urethra, pharynx, and anorectum of every homosexual man in the study. when the first cultures of pharyngeal and rectal specimens were negative, these cultures were repeated twice at weekly int ...	1978	105412
gonococcal auxotyping, a new tool.		1978	105413
[centenary of the discovery of the causative agent of gonorrhea].		1979	105505
[egg yolk medium for determination of enzymatic properties of gonococcus].		1978	105506
[comparative study of allergenic fractions extracted from different strains of staphylococci].	various methods for obtaining staphylococcus allergens were approbated. biological activity of the preparation proved to depend on the method of obtaining it determining the chemical compostition of the preparation, and on the properties of the strain used. a method of alkaline extraction and a strain with marked pathogenicity signs (strain 209) can be recommended for the preparation of staphylococcus allergen. biological activity of the allergen in case of its approbation in the same dose by pr ...	1978	105524
isolation of meningococci in meningococcal endophthalmitis.	we describe a case of bilateral hypopyon in a 7-year-old african male receiving systemic antibiotic therapy for meningococcal meningitis. aqueous from paracentesis of the left eye contained intra- and extra-cellular gram negative diplococci. we believe this is the first report of isolation of the organism since the advent of antibiotic treatment.	1979	105789
enzyme-linked immunosorbent assays for the detection of neisseria gonorrhoeae specific antibodies.	an indirect enzyme-linked immunosorbent assay (elisa) using rigid polystyrene microtiter plates was adapted to detect specific gonococcal antibodies against outer membrane-complex antigens extracted from neisseria gonorrhoeae. the concentration of antigen to obtain maximum coating of the well was 10 micrograms protein per millilitre. the optimal binding of the primary antibody and enzyme-conjugated antimmunoglobulin was achieved after 1 h at 37 degrees c. under these conditions using gonococcal ...	1978	105793
research for new antibiotics in the treatment of gonorrhea and nonspecific urethritis.		1978	105799
preservation and storage of pathogenic neisseria.	pathogenic species of neisseria, especially n. gonorrhoeae are notoriously difficult to maintain in a viable state for long storage periods. frequent transfer of cultures on artificial media and storage at 4 to 8 c quickly leads to loss of viability and virulence and to changes in antigenicity. freeze-drying has been reported as a convenient method for long-term storage of these organisms; however, many laboratories are not properly equipped to use this method. a simple, inexpensive, and efficie ...	1978	106024
rapid micro-carbohydrate test for confirmation of neisseria gonorrhoeae.	a rapid carbohydrate utilization procedure for the confirmation of neisseria gonorrhoeae and identification of other neisseria species has been developed. this method utilizes both preformed enzymes, introduced in a heavy inoculum, and enzymes formed by the microorganisms as a result of growth in a small volume of super-enriched medium. expected carbohydrate reactions were produced by 383 clinical isolates of neisseriae and were clearly visible within 4 h of incubation. the combined use of dispo ...	1978	106068
rapid diagnosis of septic arthritis by quantitative analysis of joint fluid lactic acid with a monotest lactate kit.	the monotest lactate kit (mlt) was compared with gas-liquid chromatography (glc) for the rapid detection of septic arthritis. a total of 36 joint fluids were tested. specimens were obtained from patients with septic arthritis (17 cases), inflammatory arthritis (18 cases), and degenerative arthritis (1 case). specimens from 15 patients with bacterial arthritis had lactate levels above 65 mg/dl (mean, 318 mg/dl with the glc method and 378 mg/dl with the mlt method). three specimens from patients w ...	1978	106069
bacteriological study on the saliva of healthy adults.		1979	106144
[antibioprophylaxis of meningococcal meningitis: reality or utopia? (author's transl)].	after restating the activity of the various antibiotics on neisseria meningitidis, the authors review the results of 18 experiences of germ-carriers control by antibiotics, which were rather deceiving. conditions for such a control are exposed but question of its real necessity is raised.	1978	106213
[laboratory methods used for the screening and the control of sexually transmitted diseases (author's transl)].	the most currently used methods by laboratories for the screening of sexually transmitted diseases were reviewed by the authors and their comparative reliability and sensitiivity examined. the authors endeavoured to give guidance to physicians and bacteriologists in the choice of available techniques as regards collection sites, transport of specimens, choice of transport media, serological tests for syphilis screening and diagnostic methods for urethritis or non-gonococcal vaginitis.	1977	106443
diagnosis of intra-amniotic infection.		1977	106471
[present concepts of ultrastructure of gonococcus].		1979	106576