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effect of water activity and temperature on germination and growth of penicillium digitatum, p. italicum and geotrichum candidum.this study compares the effect of temperature (4-37 degrees c) and water activity (aw: 0.99-0.87) and their interactions on the germination rates, lag times prior to germination and mycelial growth 'in vitro' of penicillium digitatum, p. italicum and geotrichum candidum, the main postharvest pathogens affecting citrus fruits.200312631189
inhibition of citrus postharvest pathogens by vapor of citral and related compounds in culture.the vapors of citral, its isomers geranial and neral, and its related compounds were examined for their effect on penicillium digitatum, penicillium italicum, and geotrichum candidum, the major fungi responsible for postharvest spoilage of citrus. vapor of citral and its two isomers generated from 15 microl l(-1) aqueous solutions in petri dishes inhibited development of the three pathogens, with concentrations of 2-6 microl l(-1) also being effective against p. italicum. vapors of citral and ge ...200312696950
isolation and cdna cloning of novel hydrogen peroxide-dependent phenol oxidase from the basidiomycete termitomyces albuminosus.a novel hydrogen peroxide-dependent phenol oxidase (tap) was isolated from the basidiomycete termitomyces albuminosus. tap is an extracellular monomeric enzyme with an estimated molecular weight of 67 kda. the purified enzyme can oxidize various phenolic compounds in the presence of hydrogen peroxide, but cannot oxidize 3,4-dimethoxybenzyl (veratryl) alcohol. mn(ii) was not required for catalysis by tap. the optimum ph for tap activity was 2.3, which is the lowest known optimum ph for a fungal p ...200312698279
ustilipids, acylated beta-d-mannopyranosyl d-erythritols from ustilago maydis and geotrichum candidum.ustilago species produce an extracellular oil that shows activity in various pharmaceutical assays. we isolated several complexes of this heterogeneous glycolipid from cultures of ustilago maydis dsm 11494 and geotrichum candidum st 002515, and determined the chemical structures of these new compounds, termed ustilipids, on the basis of nmr experiments, mass spectra, and fatty acid analyses. they all possess a 4-o-beta-d-mannopyranosyl d-erythritol basic framework, the configuration of which was ...200312715867
dual influence of the carbon source and l-methionine on the synthesis of sulphur compounds in the cheese-ripening yeast geotrichum candidum.the effect of the carbon source and l-methionine on the ability of geotrichum candidum to produce volatile sulphur compounds (vsc) was studied. this yeast was cultivated in a synthetic medium supplemented with various carbon sources and l-methionine at different concentrations. both glycerol and glucose significantly increased vsc production by g. candidum. unlike the effect on the l-methionine- and 4-methylthio-2-oxobutyric acid-demethiolating activities, the supply of a carbon source had a dra ...200312743766
staphylococcus equorum subsp. linens, subsp. nov., a starter culture component for surface ripened semi-hard cheeses.two staphylococcal strains, rp29t and rp33, were isolated from the main microflora of a surface ripened swiss mountain cheese made from raw milk. these two strains were differentiated from the most closely related species staphylococcus equorum on the basis of dna-dna hybridisation and phenotypic characteristics and are proposed as staphylococcus equorum subsp. linens subsp. nov. they could be distinguished phenotypically from s. equorum by their sensitivity to all 14 tested antibiotics, especia ...200312747407
biocatalytic reduction of ketones by a semi-continuous flow process using supercritical carbon dioxide.the immobilized resting-cell of geotrichum candidum was used as a catalyst for the reduction of a ketone in a semi-continuous flow process using supercritical carbon dioxide for the first time; it was also applied for the asymmetric reduction of a ketone and resulted in excellent enantioselectivity (ee > 99%) and a higher space-time yield than that of the corresponding batch process.200312778733
isolation of moulds capable of producing mycotoxins from blue mouldy tulum cheeses produced in turkey.a total of 16 moulds was isolated and identified from 12 blue mouldy tulum cheeses collected from retailers in erzurum, turkey; 12 were penicillium roqueforti and 4 were geotrichum candidum. the p. roqueforti isolates were grown in yeast sucrose broth at 5, 12 and 25 degrees c for 10 days, then extracted with chloroform and acetone and the extracts were examined for the presence of patulin, penicillic acid, roquefortine and pr toxin using thin layer chromatography. all of the p. roqueforti strai ...200312810273
antifungal compounds from zanthoxylum chiloperone var. angustifolium.an alkaloidal extract of the stem barks of zanthoxylum chiloperone var. angustifolium exhibited antifungal activity against candida albicans, aspergillus fumigatus and trichophyton mentagrophytes var. interdigitale using a tlc bioautographic method. bioassay-guided fractionation of this extract resulted in the isolation of two active compounds identi fi ed as canthin-6-one and 5-methoxycanthin-6-one. canthin-6-one exhibited a broad spectrum of activities against aspergillus fumigatus, a. niger, ...200312820240
[yeasts and fungi isolated at the mycology laboratory of the first dermatovenerology clinic of the medical faculty hospital of comenius university in bratislava 1995-2000].in 1995-2000 we examined in the mycological laboratory 8663 specimens for fungal infection by microscopical examination cultivation. the species was identified by the germ-tube test, fermentation and assimilation of carbohydrates. we examined 3421 specimens of faeces and found fungal infection in 37%, the most common was candida albicans and geotrichum candidum. in 1736 specimens of sputum and bronchial lavage, yeast microorganisms were isolated in 48%, mostly candida albicans. in 2697 specimens ...200312822538
cross-reactivity of the platelia candida antigen detection enzyme immunoassay with fungal antigen extracts.we studied the specificity of the platelia candida ag enzyme immunoassay by using 130 isolates of 63 clinically relevant fungal species. antigen extracts of seven candida spp. (candida albicans, c. dubliniensis, c. famata, c. glabrata, c. guilliermondii, c. lusitaniae, and c. tropicalis) repeatedly yielded positive reactions (>0.5 ng/ml). geotrichum candidum and fusarium verticillioides were found to yield borderline-positive reactions (0.25 to 0.50 ng/ml). antigen preparations from the other 54 ...200312843102
proteolytic yeasts isolated from raw, ripe tomatoes and metabiotic association of geotrichum candidum with salmonella.metabiotic associations between food-borne fungi and bacteria capable of causing human diseases are a public health concern. a survey of decayed and damaged, uncooked, ripe tomatoes was done to determine the presence and prevalence of yeasts capable of increasing the ph pulp tissue, thus creating a more favorable environment for survival and growth of enteric pathogens. sixty-two of the 371 (16.7%) fungi isolated from 215 decayed or damaged tomatoes; 12 of the 62 (19.4%) yeasts showed proteolyti ...200312892925
characterization of yeast isolates originating from hungarian dairy products using traditional and molecular identification techniques.yeast isolates collected from various hungarian dairy products were identified using simplified identification system (sim) and restriction fragment analysis of pcr-amplified 18s rdna with the neighbouring its1 region (its-pcr; ribotyping). the isolates were grouped into 26 species; geotrichum candidum, debaryomyces hansenii, yarrowia lipolytica, kluyveromyces lactis and candida catenulata were found as the predominant species. sim and its-pcr proved to be useful and convenient taxonomic tools f ...200312892927
an outbreak of fungal dermatitis and stomatitis in a free-ranging population of pigmy rattlesnakes (sistrurus miliarius barbouri) in florida.between september 1997 and march 1998, a severe skin, eye, and mouth disease was observed in a population of dusky pigmy rattlesnakes (sistrurus miliarius barbouri), at the lake woodruff national wildlife refuge in volusia county, florida (usa). three affected pigmy rattlesnakes were submitted for necropsy. all snakes had severe necrotizing and predominantly granulomatous dermatitis, stomatitis, and ophthalmitis, with involvement of the subadjacent musculature and other soft tissues. numerous fu ...200312910760
[disseminated nodular lesions due to geotrichum candidum with allergy; remarkable effect of isoniazid]. 195313094422
[on the metabolism of oospora lactis. 2. on the presence of the enzymes: glucose-6-phosphate dehydrogenase, 6-phosphogluconic dehydrogenase and phosphoglucoisomerase]. 195913833295
antigenic relationship of geotrichum candidum strains. 196213878568
cytological and physiological studies of germinating arthrospores of geotrichum candidum. 196114008908
[on geotrichum candidum and oospora lactis and their occurrence in anal eczemas]. 196314108351
identification of d-mannitol in geotrichum candidum link and the mechanism of its formation. 196414168949
[corneal mycosis caused by geotrichum candidum]. 196414212323
[on the metabolism of oospora lactis. 3. on the presence of the enzymes: phosphofructokinase and aldolase]. 195914418321
[on the metabolism of oospora lactis. 1. enzymatic transformation of ribose-5-phosphate into glucose-6-phosphate]. 195914424066
pentose metabolism by cell-free extracts of oospora lactis. 196214475918
catabolism of volatile sulfur compounds precursors by brevibacterium linens and geotrichum candidum, two microorganisms of the cheese ecosystem.two brevibacterium linens strains and the cheese-ripening yeast geotrichum candidum were compared with regard to their ability to produce volatile sulfur compounds (vscs) from three different precursors namely l-methionine, 4-methylthio-2-oxobutyric acid (kmba) and 4-methylthio-2-hydroxybutyric acid (hmba). all microorganisms were able to convert these precursors to vscs. however, although all were able to produce vscs from l-methionine, only g. candidum accumulated kmba when cultivated on this ...200314580796
submerged cultures of geotrichum candidum and penicillium camembertii on amino acids and glucose. 200314581994
mould specific igg antibodies connected with sinusitis in teachers of mould damaged school: a two-year follow-up study.the aim of this study was to describe the relationship between mould exposure induced by moisture damage and mould specific immunoglobulin g antibodies to 20 common mould species and their association with respiratory diseases.200314587535
performance of chromagar candida and biggy agar for identification of yeast species.the importance of identifying the pathogenic fungi rapidly has encouraged the development of differential media for the presumptive identification of yeasts. in this study two differential media, chromagar candida and bismuth sulphite glucose glycine yeast agar, were evaluated for the presumptive identification of yeast species.200314613587
rapid identification of fungi by sequencing the its1 and its2 regions using an automated capillary electrophoresis system.we developed a standardized dna sequence-based approach for the accurate and timely identification of medically important fungi by sequencing polymerase chain reaction (pcr) products with a rapid automated capillary electrophoresis system. a simple dna extraction method and pcr amplification using universal fungal primers was used to amplify ribosomal dna from a range of clinical isolates and reference strains. the entire internal transcribed spacer (its) 1-5.8s-its2 ribosomal dna region was seq ...200314653513
effect of water activity and temperature on competing abilities of common postharvest citrus fungi.the effect of temperature (4-30 degrees c) and water activity (a(w), 0.995-0.90) on the 'in vitro' interactions between penicillium digitatum, penicillium italicum and geotrichum candidum were evaluated. the effect of temperature on growth of green and blue mould decays and their interactions on wounded oranges was also studied. the major competing abilities were observed at optimal conditions of temperature and a(w) for growth (25 degrees c and 0.995 a(w)), and no differences between growth rat ...200414672832
exposure to bioaerosols in a municipal sewage treatment plant.microbiological air sampling was performed in a medium-size sewage treatment plant processing municipal wastewater from a city located in eastern poland. air samples for determination of the concentrations of viable mesophilic bacteria, gram-negative bacteria, thermophilic actinomycetes, fungi and endotoxin were collected at 12 sites associated with various phases of sewage treatment process. the concentrations of total mesophilic bacteria (both gram-positive and gram-negative) were within a ran ...200314677919
[herpes simplex and geotrichum candidum pneumonia in a patient with moderate renal failure].viral or fungal pneumonia generally occurs in immunocompromised patients. we report a case of hypoxic pneumonia unresponsive to broad-spectrum antibiotic therapy in a patient whose only risk factor for immunodeficiency was mild silent renal failure. the causal association between herpes simplex and geotrichum candidum was retained on the basis of endobronchial samples and the favorable course after institution of specific treatment. it is thus useful to consider these pathogens in patients with ...200314707925
geotrichum candidum septicemia in a child with hepatoblastoma. 200414743060
[production of mycoprotein by culture of oospora lactis in dealcoholized molasses waste]. 195014791378
[biochemistry of oospora lactis. v. latest research on the utilization of pentoses]. 195014791714
[formation of vitamins by oospora lactis]. 195014828337
[frequency of mycosis in immunosuppressed patients at a regional hospital of mexico city].to isolate and identify the fungi in specimens collected from immunocompromised patients seen in mexico city hospital, and to assess their association with mycosis.200314974289
differential activation of the nf-kappab-like factors relish and dif in drosophila melanogaster by fungi and gram-positive bacteria.the current model of immune activation in drosophila melanogaster suggests that fungi and gram-positive (g(+)) bacteria activate the toll/dif pathway and that gram-negative (g(-)) bacteria activate the imd/relish pathway. to test this model, we examined the response of relish and dif (dorsal-related immunity factor) mutants to challenge by various fungi and g(+) and g(-) bacteria. in relish mutants, the cecropin a gene was induced by the g(+) bacteria micrococcus luteus and staphylococcus aureus ...200414985331
identification of yeast strains isolated from a two-phase decanter system olive oil waste and investigation of their ability for its fermentation.a dynamic fed-batch microcosm system is described which permits assessment of the progressive growth of yeasts through olive oil waste. we report on its application to measure the effects of the growth of yeast strains upon the chemical composition of "alpeorujo", the waste of a two-phase decanter system used for the extraction of olive oil. six phenotypically distinct groups of yeasts were isolated. three selected isolates were identified as being most closely related to saccharomyces sp., cand ...200415062826
resin straw as an alternative system to securely store frozen microorganisms.freezing of prokaryotic and eukaryotic microorganisms is the main interest in the study of cold stress responses of living organisms. in parallel, applications which arise from this approach are of two types: (i) optimization of the frozen starters used in food processing; and (ii) improvement of the ex situ preservation of microorganisms in collections. currently, cryopreservation of microorganisms in collections is carried out in cryotubes, and bibliographical references related to freezing mi ...200415063058
mould contamination in production of semi-hard cheese.air, equipment, plastic film, brine and milk were sampled from four cheese factories in norway during the period september 1997 to may 1999 in order to identify the critical points for mould contamination in the production process. altogether, 672 samples were collected. penicillium brevicompactum was the most frequently isolated species from three of the factories, while geotrichum candidum was the most frequently isolated species from the fourth. p. commune, p. palitans, p. solitum and p. roqu ...200415135581
diffusion of lactate and ammonium in relation to growth of geotrichum candidum at the surface of solid media.geotrichum candidum was cultivated at the surface of solid model media containing peptone to simulate the composition of camembert cheese. the surface growth of g. candidum induced the diffusion of substrates from the core to the rind and the diffusion of produced metabolites from the rind to the core. in the range of ph measured during g. candidum growth, constant diffusion coefficients were found for lactate and ammonium, 0.4 and 0.8 cm(2) day(-1), respectively, determined in sterile culture m ...200415211490
airborne fungi in industrial environments--potential agents of respiratory diseases.investigations on airborne fungi in a poultry house, a swinery, a feed preparing and storing house, a grain mill, a wooden panel producing factory, and organic waste recycling facilities have been carried out in lithuania. low concentrations of fungal spores were detected in the wooden panel producing factory, the swinery, the feed preparing and storing house, and the poultry house; moderate concentrations were found in the organic waste recycling facilities; high concentrations were revealed at ...200415236494
occurrence and characterization of yeasts isolated from artisanal fiore sardo cheese.the occurrence of yeast microflora in artisanal fiore sardo cheese during ripening was studied. mean yeast counts ranged from 2.64+/-1 log(10) cfu ml(-1) in milk to 0.65+/-1 log(10) cfu g(-1) in 9 months cheese, with the higher counts observed in 48-h-old cheese. strains belonging to the prevalent species debaryomyces hansenii, kluyveromyces lactis, geotrichum candidum, candida zeylanoides and candida lambica were selected for technological and genotypic characterization. all d. hansenii strains ...200415240074
characteristics of a newly isolated fungus geotrichum candidum dec 1 with broad degradation spectrum of xenobiotic compounds.a newly isolated fungus, geotrichum candidum dec 1 (abbreviated as dec 1), was found to have the ability to degrade many xenobiotic compounds such as synthetic dyes, food coloring agents, molasses, organic halogens, lignin and kraft pulp effluents. the broad spectrum of the degradation of these compounds are associated mainly with peroxidases produced by the fungus.200315296177
crystallization and preliminary x-ray studies on candida cylindracea lipase.as part of the programme to understand the mechanism and specificity of lipase enzymes used in biotransformation reactions, the lipase from candida cylindracea has been purified and crystallized. this lipase has been widely used by organic chemists for hydrolysis and esterification reactions. crystals were obtained using polyethylene glycol 6000 as a precipitant and grew to 0.6 mm in the maximum dimension. the enzyme crystallized in the space group p2(1) with unit-cell dimensions a = 94.3, b = 1 ...199715299945
controlled production of camembert-type cheeses. part i: microbiological and physicochemical evolutions.a holistic approach of a mould cheese ripening is presented. the objective was to establish relationships between the different microbiological and biochemical changes during cheese ripening. model cheeses were prepared from pasteurized milk inoculated with kluyveromyces lactis, geotrichum candidum, penicillium camemberti and brevibacterium linens under aseptic conditions. two cheese-making trials with efficient control of environmental parameters were carried out and showed similar ripening cha ...200415354582
controlled production of camembert-type cheeses. part ii. changes in the concentration of the more volatile compounds.flavour generation in cheese is a major aspect of ripening. in order to enhance aromatic qualities it is necessary to better understand the chemical and microbiological changes. experimental camembert-type cheeses were prepared in duplicate from pasteurized milk inoculated with kluyveromyces lactis, geotrichum candidum, penicillium camemberti and brevibacterium linens under aseptic conditions. two replicates performed under controlled conditions of temperature (12 degrees c), relative humidity ( ...200415354583
microbiological quality of fermented cassava flour 'kpor umilin'.a study was conducted on the microbiological quality of traditionally fermented cassava flour 'kpor umilin' and a laboratory modification of the method developed with a view to upgrading the traditional process. microbial analysis of samples from both processing methods was evaluated using standard microbiological methods. the bacterial count in the traditionally processed flours ranged from 2.7 x 10(3) to 1.2 x 10(7) cfu/g, while the modified sample had a count of 3.5 x 10(2) cfu/g. the predomi ...200415369985
survey of yeasts for antagonistic activity against salmonella poona in cantaloupe juice and wounds in rinds coinfected with phytopathogenic molds.application of yeasts as biocontrol agents to prevent mold decay of fruits and vegetables has been described. we examined 10 yeasts for potential antagonistic activity against survival and growth of salmonella poona in cantaloupe juice and decay by cladosporium cladosporioides and geotrichum candidum in wounds on cantaloupe rind. cantaloupe juice was inoculated using five schemes: salmonella poona only (1.10 log cfu/ml), high (3.93 to 5.21 log cfu/ml) or low populations (1.79 to 3.26 log cfu/ml) ...200415508621
growth inhibition of foodborne pathogens and food spoilage organisms by select raw honeys.twenty-seven honey samples from different floral sources and geographical locations were evaluated for their ability to inhibit the growth of seven food spoilage organisms (alcaligenes faecalis, aspergillus niger, bacillus stearothermophilus, geotrichum candidum, lactobacillus acidophilus, penicillium expansum, pseudomonas fluorescens) and five foodborne pathogens (bacillus cereus, escherichia coli o157:h7, listeria monocytogenes, salmonella enterica ser. typhimurium, and staphylococcus aureus) ...200415527912
metabiotic associations of molds and salmonella poona on intact and wounded cantaloupe rind.salmonella poona, a serotype rarely implicated in human infections, has recently caused several cantaloupe-associated outbreaks of salmonellosis. metabiotic associations of molds and foodborne pathogens on produce have been reported. we tested proteolytic activity and measured changes in the ph of cantaloupe rind caused by growth of alternaria alternata, cladosporium cladosporioides, epicoccum nigrum, geotrichum candidum, and penicillium expansum. survival and growth characteristics of salmonell ...200515582743
involvement of lignin peroxidase in the decolourization of black olive mill wastewaters by geotrichum candidum.decolourization of black olive mill wastewaters (omw) by depolymerization of phenolic compounds by geotrichum candidum.200515612995
geotrichum silvicola sp. nov., a novel asexual arthroconidial yeast species related to the genus galactomyces.four strains of an asexual arthroconidial yeast species were isolated from drosophila flies in two atlantic rain forest sites in brazil and two strains from oak tasar silkworm larvae (antheraea proylei) in india. analysis of the sequences of the d1/d2 large subunit rrna gene showed that this yeast represented a novel species of the genus geotrichum, described as geotrichum silvicola sp. nov. the novel species was related to the ascogenous genus galactomyces. the closest relatives of geotrichum s ...200515657028
development and validation of a plate technique for screening of microorganisms that produce volatile sulfur compounds.volatile sulfur compounds (vscs) are of major importance for flavor development in foodstuffs such as cheeses. such compounds originate from the amino acid l-methionine, which can be degraded to methanethiol (mtl), a common precursor to a variety of other vscs. a plate assay based on double-layer petri dishes containing 5,5'-dithio-bis-2-nitrobenzoic acid (dtnb), a chemical used for the estimation of free thiols, in the upper layer provides an easy and reliable detection method for thiol-produci ...200515745751
efficacy assessment of candida oleophila (strain o) and pichia anomala (strain k) against major postharvest diseases of citrus fruits in morocco.two yeasts, candida oleophila (strain o) and pichia anomala (strain k), were previously selected for their antagonistic activity against postharvest diseases on apples and pears. the objective of the study was to determine the efficacy of both antagonistic yeast's against wound postharvest pathogens of citrus fruits. the efficacy of both strains (applied at 10(5), 10(6) and 10(8) cfu/ml) was assessed against penicillium digitatum and p. italicum inoculated after one hour (at a concentration of 1 ...200415756846
pulmonary hyalohyphomycosis caused by fusarium spp in a kemp's ridley sea turtle (lepidochelys kempi): an immunohistochemical study.to describe the microscopic and immunohistochemical findings in a case of pulmonary hyalohyphomycosis in a kemp's ridley sea turtle (lepidochelys kempi).200415768112
[penicillium-inhibiting yeasts].the objective of this work was to establish the in vitro and in vivo inhibition of post-harvest pathogenic moulds by yeasts in order to make a biocontrol product. post-harvest pathogenic moulds penicillium digitatum, p. italicum, p. ulaiense, phyllosticta sp., galactomyces geotrichum and yeasts belonging to genera brettanomyces, candida, cryptococcus, kloeckera, pichia, rhodotorula were isolated from citrus fruits. some yeasts strains were also isolated from other sources. the yeasts were identi ...200415786872
isolation, identification and antifungal susceptibility of lemon pathogenic and non pathogenic fungi.numerous species of filamentous fungi were isolated from lemon on different plantations in the province of tucuman, argentina. the techniques suggested by the subcommittee of antifungal susceptibility of the national committee for clinical laboratory standards, (usa) were adapted. the effect of three different concentrations of the fungicides imazalil, guazatine, sopp and thiabendazole on the fungi fusarium oxysporum, fusarium moniliforme, aspergillus niger, aspergillus flavus, aspergillus clava ...200515813686
interests in geotrichum candidum for cheese technology.the wide genotypic and phenotypic diversity of geotrichum candidum strains does not facilitate its classification as yeast or a yeast-like fungus that is still a matter of debate. whatever its classification, g. candidum possesses many different metabolic pathways that are of particular interest to the dairy industry. g. candidum is of importance in the maturation of cheese, and much is known about its direct contribution to cheese ripening and flavour formation. its diverse metabolic potential ...200515924999
evaluation of mycological contamination of dental unit waterlines.the quality of dental unit water is of great importance since patients and dental staff are regularly exposed to water from aerosols generated during work. the main purpose of this investigation was mycological evaluation of dental unit waterlines (duwl). the author determined the number and species of fungi present in the water from a unit reservoir which is the source of water for a dental unit, in the water flowing from a high-speed handpiece of a unit, and in the swab sample collected from t ...200516028882
cdna cloning and genetic analysis of a novel decolorizing enzyme, peroxidase gene dyp from geotrichum candidum dec 1.a novel decolorizing peroxidase gene (dyp) was cloned from a cdna library of a newly isolated strain of fungus geotrichum candidum dec 1. the open reading frame of 1494 nucleotides which corresponds to dyp predicts a primary translation product of 498 amino acids, m(r) 53,306. the deduced amino acid sequence of dyp does not contain the typical conserved motif which is characteristic of heme-containing peroxidases in the plant peroxidase superfamily. comparison of the deduced amino acid sequence ...199916232492
batch decolorization of molasses by suspended and immobilized fungus of geotrichum candidum dec 1.geotrichum candidum dec 1, which exhibits a broad dye-decolorizing spectrum, was used to decolorize molasses during semi-batch cultivation. an eighty % decolorization of molasses and a stable peroxidase activity were maintained for approximately four weeks, after which, both activities deteriorated significantly. subsequently, repeated batch cultivation of fungal cells immobilized on polyurethane foam was employed to solve the aforementioned problems and stable decolorization of molasses as well ...199916232668
effect of molasses on the production and activity of dye-decolorizing peroxidase from geotrichum candidum dec1.the production of dye-decolorizing peroxidase (dyp) was investigated by cultivating geotrichum candidum dec1 using molasses as a carbon source. molasses at concentrations greater than 10 g.l(-1) was found to increase the decolorization activity of the culture broth toward dye, reactive blue 5 mainly because the amount of enzyme produced was enhanced. however, complete inhibition of dyp activity by molasses was observed at the concentration of 20 g.l(-1), indicating that the inhibitory effect of ...200016232795
characterization of aryl alcohol oxidase produced by dye-decolorizing fungus, geotrichum candidum decl.aryl alcohol oxidase (aao) produced by geotrichum candidum dec1 (dec1), a newly isolated decolorizing fungus, was purified by ultrafiltration and by using diethylaminoethyl (deae) sephacel, butyl-toyopearl and mono-q columns. h2o2 produced by concomitant aao oxidation of veratryl alcohol (va) to veratraldehyde was consumed by a peroxidase (dyp) purified from dec1 culture, leading to the decolorization of a dye (in vitro). in the liquid culture of dec1, the existence of h2o2 and veratraldehyde wa ...200116232969
a new model for the reconstruction of biomass history from carbon dioxide emission during batch cultivation of geotrichum candidum.a non-structured model has been developed to describe the co2 emission during growth of geotrichum candidum on a lactate + peptone-based liquid medium. from the nitrogen and carbon mass balances, it was shown that about 50% of the total co2 released was from the metabolism of the energy supply for biosynthesis, and the remaining from that for maintenance; thus, co2 production was considered to be partially associated with growth. the model fitted the experimental data as long as a net growth was ...200116233041
efficient production of a heterologous peroxidase, dyp from geotrichum candidum dec 1, on solid-state culture of aspergillus oryzae rd005.the productivity of a peroxidase (dyp) originating from geotrichum candidum dec 1 was enhanced in the solid-state culture using aspergillus oryzae rd005. when the humidity, water content, and temperature were adjusted to 60%, 50% and 27 degrees c, respectively, the productivity of dyp reached 5.3 g per kilogram wheat bran, which was used as the solid medium. the yield of 5.3 g per kg wheat bran corresponded to the yield of a 56 kg submerged culture. the productivity per gram carbon of the medium ...200116233153
olive-mill wastewaters: a promising substrate for microbial lipase production.the present study investigated the valorization of olive-mill wastewater (omw) by its use as a possible growth medium for the microbial production of extra-cellular lipase. to this end, strains of geotrichum candidum (nrrl y-552 and y-553), rhizopus arrhizus (nrrl 2286 and isrim 383), rhizopus oryzae (nrrl 6431), aspergillus oryzae (nrrl 1988 and 495), aspergillus niger (nrrl 334), candida cylindracea (nrrl y-17506) and penicillium citrinum (nrrl 1841 and 3754, isrim 118) were screened. all stra ...200616236495
role of geotrichum candidum in canine oral ulcers. 200516309361
fungal and enzymatic decolourisation of artificial textile dye baths.a textile dye reactive black 5 was used in screening 25 fungal strains for their decolourising ability. the most promising strains were tested in a medium containing specific constituents of a dye bath in order to approach real application conditions. it was shown that the concentrations of the constituents had to be reduced to allow fungal growth. decolourisation started in cultures of geotrichum candidum but was not complete. only bjerkandera adusta was able to decolourise the black-blue colou ...200616310823
synthesis and evaluation of demethoxyviridin derivatives as potential antimicrobials.the in vitro antibacterial and antifungal activities of demethoxyviridin and some synthetic analogues were evaluated by the agar diffusion method. the minimum inhibitory concentrations (mic) of the active compounds were also determined by the agar dilution method. demethoxyviridin (1) showed moderate antibacterial activity against most of the strains tested. 1alpha-hydroxydemethoxyviridin (3) showed antibacterial activity and the most potent in vitro antifungal activity with mic of 20 microg/ml ...200516320609
evaluation of the role of syringomycin in plant pathogenesis by using tn5 mutants of pseudomonas syringae pv. syringae defective in syringomycin production.syringomycin is a necrosis-inducing phytotoxin produced by pseudomonas syringae pv. syringae. to determine whether syringomycin production is a determinant in virulence or pathogenicity, we isolated nontoxigenic (tox) tn5-containing mutants and then quantitatively evaluated them for the ability to multiply and cause disease in immature sweet-cherry fruits. transposon tn5 was delivered to tox strain b301d-r by using the suicide vector, pgs9, and the resultant kanamycin-resistant (km) colonies wer ...198816347644
immobilization of leachable toxic soil pollutants by using oxidative enzymes.screening of leachable toxic chemicals in a horseradish peroxidase-h(2)o(2) immobilization system established that immobilization was promising for most phenolic pollutants but not for benzoic acid, 2,6-dinitrocresol, or dibutyl phthalate. the treatment did not mobilize inherently nonmobile pollutants such as anilines and benzo[a]pyrene. in a separate study, an extracellular laccase in the culture filtrate of geotrichum candidum was selected from five fungal enzymes evaluated as a cost-effective ...198816347683
scanning electron and light microscopic study of microbial succession on bethlehem st. nectaire cheese.st. nectaire cheese is a semisoft cheese of french origin that, along with brie and camembert cheeses, belongs to the class of surface mold-ripened cheese. the surface microorganisms that develop on the cheese rind during ripening impart a distinctive aroma and flavor to this class of cheese. we have documented the sequential appearance of microorganisms on the cheese rind and in the curd over a 60-day ripening period. scanning electron microscopy was used to visualize the development of surface ...199216348797
[evaluation of species distribution of yeasts isolated from intensive care units during the four years period].the aim of this study was to evaluate the distributions of yeast species according to the years and to detect the emerging pathogens in intensive care units (icu). for this purpose, yeast isolation rates were detected retrospectively, in the following time periods: period i: april-december 2001; period ii: january-december 2002; period iii: january-december 2003; period iv: january-december 2004. a total of 490 yeast isolates recovered from 462 clinical specimens obtained from 360 different icu ...200516358490
in vitro degradation of equine keratin by dermatophytes and other keratinophilic fungi.keratinolytic properties of two dermatophytes (microsporum gypseum, trichophyton mentagrophytes) and three moulds (scopulariopsis brevicaulis, alternaria alternata, geotrichum candidum) isolated from diseased equine hooves were examined to improve the understanding of pathogenic mechanisms leading to equine onychomycosis. equine hoof horn material and skin, as well as hoof keratin and dermal keratin extracted from corresponding tissues, were used as sole carbon and nitrogen sources in five test ...200616377103
characterization of some yeasts isolated from foods by traditional and molecular tests.in this study, 22 yeast strains isolated from foods were characterized by traditional and molecular techniques. with the help of traditional identification tests, yeast strains were grouped in 12 species belonging to 11 genera as follows: candida parapsilosis, rhodotorula mucilaginosa, debaryomyces hansenii, cryptococcus humicolus, cryptococcus albidus, aureobasidium spp., hanseniaspora valbyensis, metschnikowia pulcherrima, lachancea thermotolerans, pichia anomala, geotrichum candidum and yarro ...200616388865
mycotic dermatitis in captive carpet snakes (morelia spilotes variegata).geotrichum candidum was isolated from necrotic skin lesions in one of three captive carpet snakes (morelia spilotes variegata). hyphae and arthrospores morphologically consistent with this organism were present in histological preparations of lesions from the three snakes.197616498888
evidence for distinct l-methionine catabolic pathways in the yeast geotrichum candidum and the bacterium brevibacterium linens.tracing experiments were carried out to identify volatile and nonvolatile l-methionine degradation intermediates and end products in the yeast geotrichum candidum and in the bacterium brevibacterium linens, both of which are present in the surface flora of certain soft cheeses and contribute to the ripening reactions. since the acid-sensitive bacterium b. linens is known to produce larger amounts and a greater variety of volatile sulfur compounds (vscs) than the yeast g. candidum produces, we ex ...200616517666
polymicrobial keratomycosis in a three-year-old child.to describe a previously unreported case of polymicrobial mycotic keratitis caused by an association between candida lusitaniae, c. parapsilosis, and geotrichum candidum.200616597544
production of natural fruity aroma by geotrichum candidum.based on its aromatic potential, geotrichum candidum isolated from olive vegetation water was tested for the production of volatile compounds. when g. candidum was cultivated on media with glucose as the carbon source, flavor volatile compounds were produced and accumulated in the broth. fruity flavoring compounds (pineapple-like) such as esters and alcohols were analyzed by gas chromatography coupled to mass spectrometry, including ethyl esters of acetic acid and butyric acid, methyl-3-butan-1- ...200616632883
effect of urea on ammonium-dependent synthesis of carbamyl phosphate during spore germination of geotrichum candidum.the specific activities of enzymes catalyzing the ammonium-dependent carbamyl phosphate synthesis (nh(3)-cps) and the glutamine-dependent carbamyl phosphate synthesis (gln-cps) were increased during germination by approximately 5-and 1.7-fold respectively in the presence of 35 mm urea. the increase of nh(3)-cps and gln-cps levels occurred immediately after the onset of germination and prior to the appearance of germ tube. ammonium also stimulated the nh(3)-cps activity, but the induction caused ...197216658234
regulation of nicotinamide adenine dinucleotide phosphate-specific glutamate dehydrogenase in germinated spores of geotrichum candidum.germinating spores of geotrichum candidum produce only a nicotinamide adenine dinucleotide phosphate-linked glutamate dehydrogenase. synthesis of glutamate dehydrogenase was repressed by the presence of ammonia, whereas urea, glutamate, or glutamine were ineffective. the enzyme was not subject to catabolite repression and was localized in the cell sap fraction. the glutamate dehydrogenase has been purified 93-fold and showed maximal activity at ph 8.2 in the forward and reverse directions. when ...197316658426
microbial diversity and succession during the manufacture and ripening of traditional, spanish, blue-veined cabrales cheese, as determined by pcr-dgge.the diversity and dynamics of the dominant microbial communities arising during the manufacture and ripening of four batches of naturally fermented cabrales cheese were investigated by the pcr-dgge culture-independent technique. total microbial dna was extracted from cheese milk, curd and cheese samples and used as template material in pcr experiments to amplify the v3 region of the bacterial 16s rrna gene, plus the d1 region of the eukaryotic 26s rrna gene. these regions were then analysed usin ...200616806553
protoplast fusion between geotrichum candidium and phanerochaete chrysosporium to produce fusants for corn stover fermentation.lignin impedes the digestion of corn stover when used as an animal feed. phanerochaete chrysosporium is an efficient lignindegrader. geotrichum candidum can be used to produce single-cell protein. in this study, protoplasts of the two fungi were prepared and fused. after screening, one of the fusants, fusant r1, was selected for corn stover fermentation. it decreased lignin from 109 to 54 g/kg and increased protein from 48 to 67 g/kg in corn stover. comparison with their parental strains indicat ...200616820977
microbiological and biochemical aspects of camembert-type cheeses depend on atmospheric composition in the ripening chamber.camembert-type cheeses were prepared from pasteurized milk seeded with kluyveromyces lactis, geotrichum candidum, penicillium camemberti, and brevibacterium aurantiacum. microorganism growth and biochemical dynamics were studied in relation to ripening chamber co(2) atmospheric composition using 31 descriptors based on kinetic data. the chamber ripening was carried out under 5 different controlled atmospheres: continuously renewed atmosphere, periodically renewed atmosphere, no renewed atmospher ...200616840644
exposure to airborne fungi during conservative dental treatment.the aim of the study was a mycological assessment of bioaerosol forming during conservative dental treatment, taking into account concentration and type of fungal microflora, and evaluation of the influence of duwl disinfecting protocol on the fungal contamination of the bioaerosol. the research was conducted on 25 operative sites located in public dental clinics. the air contained in the space between a patient and a dentist during conservative dental treatment with the use of a high-speed hand ...200616841889
identification of geotrichum candidum at the species and strain level: proposal for a standardized protocol.in this study, the m13 primer was used to distinguish geotrichum candidum from the anamorphic and teleomorphic forms of other arthrospore-forming species (discriminatory power = 0.99). for intraspecific characterization, the gata4 primer showed the highest level of discrimination for g. candidum among the 20 microsatellite primers tested. a molecular typing protocol (dna concentration, hybridization temperature and type of pcr machine) was optimized through a series of intra- and interlaboratory ...200616855820
[multifocal mycoses in women: prevalence, species characteristics, and some intraspecific features].the aim of this study was to detect fungi in three ontocenoses of 947 women (aged 20-45). the prevalence of multifocal fungal infections in women varied from 62.2 +/- 2.99% to 81.0 +/- 3.92% in different ontocenoses. they assumed the following mean values: 38.4 +/- 1.77% for the vagina, 42.2 +/- 1.80% for the oral cavity, and 25.0 +/- 1.58% for the anus. the clinical manifestations of sexual organs inflammations were statistically significantly (p < 0.01) more frequent in women infected with fun ...200416865940
structure determination of neoefrapeptins a to n: peptides with insecticidal activity produced by the fungus geotrichum candidum.the structures of neoefrapeptins a to n, peptides with insecticidal activity, were elucidated. they showed a close similarity to efrapeptin. however, all neoefrapeptins contained the very rare amino acid 1-amino-cyclopropane-carboxylic acid and some of them also contained (2s,3s)-3-methylproline. the neoefrapeptins are the first case, in which these amino acids are found as building blocks for linear peptides. they were identified by comparison of the silylated hydrolyzate to reference material ...200616883776
high-level expression and characterization of galactomyces geotrichum (bt107) lipase i in pichia pastoris.the mature lipi gene, encoding the lipase i from galactomyces geotrichum bt107, was obtained by pcr from genomic dna, sequenced and cloned into a pichia pastoris expression vector. clones containing multiple copies of lipi integrated in their genome were analyzed to achieve high-level expression of the recombinant lipase i. one strain with four or more copies of the expression cassette was able to produce more than 200mg/l of extracellular heterologous protein. the lipase i was partially purifie ...200616884921
geotrichum candidum link 1809: hydrolases activity and own method of digestive tract strains biotyping.hydrolase activity of (enzymograms, biotypes) in geotrichum candidum, one of the poorly described pathogenic fungi, was studied 81 strains were isolated from oral cavity and faeces of patients with gastrointestinal tract disorders. axenic strains were differentiated with api 20c aux and api zym tests. then, enzymograms and biotypes were determined for all strains based on the activity of 19 hydrolases. high variability of enzymograms (17 different types) was found. the highest activity was noted ...200116886438
[the cytochemical evaluation of activity of some oxidoreductases of geotrichum candidum link under the influence of chemical compounds inhibiting the growth of the fungus].it has been investigated, with the aid of routine cytochemical methods, whether any changes of the activity of oxydoreductases of g. candidum which has been influenced by chemical factors affecting its growth, and with which it can contact in human organism or his environment, occur. the factors were: antibacterial antibiotic from tetracycline group/chlortetracycline/, antifungal antibiotic representing polyenes derivatives/nystatine/, heavy metal salt/mercuric chloride/, aniline dye/malachite g ...200116894743
[contamination of dairy products by yeasts-like fungi].evaluation of the degree of contamination of diary products with potentially pathogenic fungal strains. materials for the study were: yoghurt, cottage cheese, homogenised cottage cheese, cream and buttermilk. differentiation of species was based on their morphological features and biochemical properties (api 20 c biomérieux). among 300 studied diary products we have isolated 100 (33.0 +/- 2.72%) strains of yeast-like fungi. diary products were contamined with 19 species of fungi from types: cand ...200116894745
[some aspects of fungi multifocal invasions connected with the genital organs in women].the aim of presented study was analysis of fungal invasions of the genital organs, oral cavity and digestive tract in 799 women. fungal strains were identified using api 20c and api 20c aux (biomérieux); they were as follows: candida albicans, c. krusei, c. tropicalis, c. guilliermondii, c. kefyr, c. glabrata, c. famata, c. parapsilosis, rhodotorula rubra, geotrichum candidum. the number of fungal strains isolated from the oral cavity ontocenosis was the highest (41.8 +/- 2.4%). fungal strains i ...200116897965
identification of yeasts associated with milk products using traditional and molecular techniques.an integrated approach including phenotypic (morphological, biochemical and physiological characterization) and genotypic (rapd-pcr, sequencing of d1/d2 domain of 26s rrna encoding gene) methods was used for the identification of yeasts isolated from different milk products. there were 513 isolates in all, 460 ascomycetous and 53 basidiomycetous yeasts. the yeast isolates were characterized on the basis of their biochemical and physiological properties, and the d1/d2 domain of 26s rdna was seque ...200616943023
use of molecular methods for the identification of yeast associated with table olives.a molecular approach is used for the identification of yeast isolated from table olives. our results validate those obtained in the past by the classical biochemical methodology. yeast were isolated from both aerobically and anaerobically processed black table olives and also from canned seasoned green table olives. molecular identification methodology used included restriction pattern analysis of both pcr-amplified 5.8s rrna gene and internal transcribed spacers its(1) and its(2). for some spec ...200616943084
levels of cystathionine gamma lyase production by geotrichum candidum in synthetic media and correlation with the presence of sulphur flavours in cheese.geotrichum candidum is a cheese-ripening agent with the potential to produce sulphur flavour compounds in soft cheeses. we aimed to develop an alternative test for predicting the aromatic (sulphur flavours) potential of g. candidum strains in soft cheese. twelve strains of g. candidum with different levels of demethiolase activity (determined by a chemical method) in yel-met (yeast extract, lactate methionine) medium were studied. we investigated cgl (cystathionine gamma lyase) gene expression a ...200716973233
improvement of carbonyl reductase production of geotrichum candidum for the transformation of 1-acetonaphthone to s(-)-1-(1'-napthyl) ethanol.the aim of this work was to study the influence of media components and physico-chemical parameters on the growth and carbonyl reductase production by geotrichum candidum. under optimized conditions, the conversion of the substrate increased to >93%, while the specific growth rate and enzyme activity were increased by 200% and 29%, respectively. the rate of conversion of the substrate was also very high in the cells grown in optimized medium. the volumetric productivity of the biotransformation ...200717027259
[moulds and yeasts in bottled water and soft drinks].some damaged cartons of soft drinks and carbonated water were analyzed to detect the microorganisms that caused the damage. the contaminants of sugar used in the production of one of the drinks were also studied. the methods of déak & beuchat and pitt & hocking were used for the identification of yeasts and moulds, respectively. the agents of the spoilage of soft drinks were debaryomyces hansenii, debaryomyces polymorphus, galactomyces geotrichum, metschnikowia pulcherrima, mucor circinelloides, ...200617037258
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