Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| characterization of a legionella pneumophila extracellular protease exhibiting hemolytic and cytotoxic activities. | a preliminary screening of selected legionella species for proteolytic and hemolytic phenotypes suggested a correlation between these activities. to investigate the relationship of these phenotypes, a mutant strain of legionella pneumophila deficient in the expression of a 38-kilodalton (kda) exoprotease was isolated and characterized. this strain, designated prt8, was also found to be nonhemolytic when screened on blood agar composed of 5% canine or guinea pig erythrocytes. strain prt8 was sero ... | 1989 | 2917785 |
| the level of z-dna in metabolically active, permeabilized mammalian cell nuclei is regulated by torsional strain. | permeabilized nuclei from mammalian cells encapsulated within agarose microbeads in an isotonic buffer are active in transcription and replication (jackson, d. a., and p. r. cook. 1985. embo (eur. mol. biol. organ.) j. 4:913-918). their dna is intact and the nuclei are accessible to macromolecules. myeloma nuclei prepared in this way were used to probe the extent of dna negative supercoiling and the effects of altering torsional strain by binding radioactively labeled monoclonal antibodies to z- ... | 1989 | 2921282 |
| nosocomial clonal dissemination of methicillin-resistant staphylococcus aureus. elucidation by plasmid analysis. | an outbreak of methicillin sodium-resistant staphylococcus aureus (mrsa) infection and colonization, mainly centered in the vascular surgery service, occurred in a 1000-bed tertiary care center between december 1983 and december 1984. methicillin-resistant s aureus isolated before and during the outbreak was studied by both bacteriophage typing and by restriction endonuclease digestion of bacterial plasmid dna. bacteriophage typing was discrepant in nine (56%) of the 17 repeated analyses compare ... | 1987 | 2950835 |
| presence of markers for virulence in the unique short region or repeat region or both of pseudorabies hybrid viruses. | the unique short region and part of the repeat region of virulent pseudorabies virus strain nia-3 was replaced by the corresponding region of the avirulent nia-4 strain by transfection with subgenomic dna fragments. the resulting hybrid virus showed a reduced virulence in both mice and pigs. therefore, important markers for virulence are located in the unique short or repeat region or both of pseudorabies virus. we provide evidence that the terminally located repeat is not required for the gener ... | 1985 | 2981366 |
| transduction of virulence in herpes simplex virus type 1 from a pathogenic to an apathogenic strain by a cloned viral dna fragment. | herpes simplex virus (hsv) type 1 strain hfem, the genome of which contains a deletion of about 4 kilobasepairs (kbp) between 0.7 and 0.8 viral map units, is apathogenic in the tree shrew when the animals are inoculated intravenously, intraperitoneally and/or subcutaneously. similar results were obtained using balb/c mice. studies of the state of viral latency in animals infected with hsv-1 strain hfem revealed that this strain was unable to colonize the ganglia of tree shrews. infectious virus ... | 1985 | 2982083 |
| [modification of the modification-restriction system in staphylococci]. | a new system of host specificity of dna, called sau67 according to the available nomenclature, was identified in staphylococcus aureus 6782 strain by means of cross titration with staphylophage 729 considering that the phage exhibited the highly effective absorption properties. a total preparation of sau67 methylases was isolated using ammonium sulfate fractionation. the enzyme preparation contained methylases of cytosine and adenine, where the activity of adenine methylases constituted only for ... | 1985 | 2984842 |
| isolation and characterization of tn5 insertion mutants of erwinia chrysanthemi that are deficient in polygalacturonate catabolic enzymes oligogalacturonate lyase and 3-deoxy-d-glycero-2,5-hexodiulosonate dehydrogenase. | mutants of erwinia chrysanthemi ec16 deficient in the polygalacturonate catabolic enzymes oligogalacturonate lyase (ogl-) and 3-deoxy-d-glycero-2,5-hexodiulosonate (ketodeoxyuronate) dehydrogenase (kdud-) were obtained by tn5 mutagenesis using the r plasmid pjb4ji. ogl- exu+ (exu+, d-galacturonate utilization) and kdud- exu- strains macerated potato tuber tissue and utilized glucose, glycerol, and gluconate, but they did not utilize polygalacturonate, unsaturated digalacturonate, or saturated di ... | 1985 | 2985544 |
| molecular cloning of the delta-endotoxin gene of bacillus thuringiensis var. israelensis. | a transformant of bacillus megaterium, vb131, was isolated which carries a 6.3-kb xbai segment of the crystal toxin gene of bacillus thuringiensis var. israelensis (bti) cloned in a vector plasmid pbc16 to yield pvb131. the chimeric plasmid dna from vb131 was introduced into a transformable bacillus subtilis strain by competence transformation. both the b. megaterium vb131 strain and the b. subtilis strain harboring the chimeric plasmid produced irregular, parasporal, phase-refractile, crystalli ... | 1985 | 2987085 |
| inhibition of herpes simplex virus type 1 penetration by cytochalasins b and d. | internalization of herpes simplex virus type 1 (hsv) kos strain by hep-2 cells was reversibly inhibited by pretreatment of cells with cytochalasins b and d. internalization of virus following preincubation at 4 degrees c and temperature shift to 37 degrees c was normally preceded by a 5 to 8 min lag period and was complete within 20 to 30 min. a similar lag period followed hsv addition at 37 degrees c. cytochalasin d was fivefold more active on hsv entry than cytochalasin b, with 50% inhibition ... | 1985 | 2991432 |
| direct and indirect gene replacements in aspergillus nidulans. | we performed three sets of experiments to determine whether cloned dna fragments can be substituted for homologous regions of the aspergillus nidulans genome by dna-mediated transformation. a linear dna fragment containing a heteromorphic trpc+ allele was used to transform a trpc- strain to trpc+. blot analysis of dna from the transformants showed that the heteromorphic allele had replaced the trpc- allele in a minority of the strains. an a. nidulans trpc+ gene was inserted into the argb+ gene, ... | 1985 | 2991748 |
| ectopic peptides released by a human melanoma cell line that modulate the transformed phenotype. | cells derived from a human melanoma strain by low-serum selection in monolayer were found to be capable of growth in semisolid medium, forming colonies ranging from tight to loose or dispersed. these phenotypes were found to be stable after cloning and retesting. examination of the biological activities of ectopic peptides produced by a clone that gives rise to loose dispersed colonies revealed the presence of a transforming growth factor beta (tgf-beta; apparent mr 14,700) and a single tgf-alph ... | 1985 | 2991904 |
| [metabolic characteristics of polyphosphates and other macroergic phosphorus compounds in relation to the degree of penicillin production and growth conditions of penicillium chrysogenum]. | metabolism of macroergic phosphorus compounds was studied in high- and low-productive isogenic strains of penicillium chrysogenum. it was shown that the levels of the high-polymer polyphosphates (fractions pp1, pp2 and pp3) in the strain intensively producing penicillin were 2-3 times higher than those in the low-productive strain by the 2nd day of the fermentation process (the period of penicillin production). the levels of pyrophosphate and atp in the mycelium during the fermentation process d ... | 1985 | 2998269 |
| in vitro protective effect of bacteria-derived bovine alpha interferon i1 against selected bovine viruses. | we used bacteria-derived bovine alpha-interferon i1 (bo ifn-alpha i1) to study its antiviral effect in a bovine turbinate cell line on bovine diarrhea virus, infectious bovine rhinotracheitis virus, parainfluenza 3 virus, and pseudorabies virus. we based our study upon replicate tests for each strain by using a block titration system with various concentrations of bo ifn-alpha i1 against various concentrations of virus. the data were compiled in two-axis tables (replicate x concentration) and we ... | 1985 | 2999188 |
| ectopic peptides released by a human melanoma cell line that modulate the transformed phenotype. | cells derived from a human melanoma strain by low serum selection in monolayer were found to be capable of growth in semi-solid medium, forming colonies ranging from tight, to loose, or dispersed. these phenotypes were found to be stable on cloning and retesting. examination of the serum-free media conditioned by these clones indicates that the clones release activities capable of inducing agar colonies, in an indicator cell line (nrk, 49f), that express phenotypes similar to those of the melano ... | 1985 | 3000706 |
| biochemical characterization of a foot-and-mouth disease virus strain attenuated for cattle. brief report. | wild-type, virulent (a-24 cruzeiro subtype) foot-and-mouth disease virus (fmdv), a related attenuated strain and revertants of the attenuated strain were examined by titration on primary bovine kidney (pbk) and baby hamster kidney (bhk-21) cells, as well as, by infection of unweaned mice. wild type virus grew equally well in all three systems, whereas the attenuated strain had a titer 2-3 log lower in pbk cells than in the other 2 assays. within 9 successive passages in bhk-21 cells the attenuat ... | 1986 | 3006639 |
| [studies on new adenovirus types of subgenus d causing conjunctivitis--antigenic and restriction endonuclease analysis of adenovirus types 19 and 37]. | neutralization test and dna restriction endonuclease analysis were performed in strains of adenovirus types 19 (ad-19) and 37 (ad-37) isolated from patients with acute conjunctivitis in sapporo, kao-hsiung (taiwan) and pusan (korea) in the period of 1979 and 1984. although ad-19 and ad-37 were differentiated, they were related to each other in neutralization test. there was no difference between prototype strain and isolates in ad-19 and ad-37 strains. by dna restriction endonuclease analysis ad ... | 1986 | 3017830 |
| polyomavirus mutation that confers a cell-specific cis advantage for viral dna replication. | the structural and biological properties of a polyomavirus mutant selected in friend erythroleukemic cells were investigated. the growth efficiency of this mutant (pyfl78) was compared with that of the parental pya2 strain by a growth competition assay in friend erythroleukemic and 3t3 (or 3t6) cell lines. the results reveal that pyfl78 displays a cis-acting growth advantage over the pya2 parental strain in friend erythroleukemic cells but not in 3t3 or 3t6 cells. this cell-specific cis advantag ... | 1985 | 3018552 |
| susceptibilities of phosphonoacetic acid and acyclovir resistant varicella-zoster virus mutants to 9-beta-arabinofuranosyladenine and 1-beta-arabinofuranosylcytosine. | the dna polymerase activity, and susceptibilities to 9-beta-d-arabinofuranosyladenine(ara-a) and 1-beta-arabinofuranosylcytosine(ara-c) of a phosphonoacetic acid resistant mutant (paa-r) of varicella-zoster virus (vzv) selected in the presence of paa were examined. the dna polymerase activity of paa-r was inhibited less than that of the parent strain by paa in vitro. paa-r was resistant to acyclovir and also to both ara-a and ara-c. the susceptibilities to ara-a and ara-c of four acyclovir resis ... | 1986 | 3022709 |
| lactose and melibiose metabolism in erwinia chrysanthemi. | a lac+ mutant of erwinia chrysanthemi was isolated from the lac- wild type on lactose agar. beta-galactosidase was expressed independently of lactose transport in both the mutant and the wild type, and neither strain expressed thiogalactoside transacetylase. lactose transport and alpha-galactosidase, constitutive in the lac+ strain, were coordinately induced in the lac- strain by melibiose and raffinose but not by isopropyl-beta-d-thiogalactopyranoside or thiomethyl-beta-d-galactopyranoside. mel ... | 1986 | 3023289 |
| characterization of nonattaching mutants of agrobacterium tumefaciens. | the first step in tumor formation by agrobacterium tumefaciens is the site-specific binding of the bacteria to plant host cells. transposon mutants of the bacteria which fail to attach to carrot suspension culture cells were isolated. these mutants showed no significant attachment to carrot cells with either microscopic or viable cell count assays of bacterial binding. the nonattaching mutants were all avirulent. when revertants of the mutants were obtained by enriching for bacteria which do bin ... | 1987 | 3025176 |
| genome location and identification of functions defective in the bartha vaccine strain of pseudorabies virus. | we have shown previously (lomniczi et al., j. virol. 52:198-205, 1984) that the bartha vaccine strain of pseudorabies virus has a deletion in the short unique (us) region of its genome--a deletion that is related to the absence of virus virulence. this strain is, however, also defective in other genes involved in virulence. we show here that virulence can be restored by marker rescue of the bartha strain to which an intact us has been restored (but not to the parental bartha strain) by sequences ... | 1987 | 3027406 |
| genomic variation among avian rotavirus-like viruses detected by polyacrylamide gel electrophoresis. | the genomes of five turkey and two chicken rotavirus-like virus (rvlv) strains were compared with the genome of a reference turkey rvlv strain by co-electrophoresis in polyacrylamide gels. the genomes of these avian rvlv strains could be distinguished from the reference strain genome by differences in the mobilities of from three to 10 segments. | 1986 | 3028359 |
| intracerebral infection of cebus apella with the xj-clone 3 strain of junín virus. | to assess the usefulness of the south american primate cebus apella as a model for neurovirulence of junín virus, eight monkeys were inoculated with 10(5) ld50 of the attenuated xj-clone 3 junín virus strain by the intrathalamic route. after the second week, weight loss and polyadenopathies were observed in most animals, one-half of which had a transient leukothrombocytopenia. moderate clinical central nervous system (cns) involvement was present in four of eight monkeys, while the rest had only ... | 1987 | 3031201 |
| use of microplate cell culture and enzyme immunoassay in titration of serum neutralizing antibody against hochi strain of serotype 4 human rotavirus. | the tube neutralization test read by enzyme immunoassay developed by wyatt et al. (1983) for serotype determination of human rotavirus was modified so as to use stationary cultures of ma104 cells in a microtiter plate instead of roller tube cultures. sera obtained from different age groups were titrated for neutralizing antibody against serotype 4 human rotavirus hochi strain by this test and the results were compared with those obtained by the plaque neutralization test. there was a good correl ... | 1986 | 3035256 |
| differentiation of poliovirus type i isolates in the 1982 epidemic from sabin-i strain by antigenic and temperature markers. | poliovirus type 1 strains recovered from patients with poliomyelitis during the epidemic of 1982, were distinct antigenically from sabin-1 strain by comparing homologous and heterologous neutralization rate constants of sabin-1 specific antiserum. with the reproductive capacity at 40 degrees c, these isolates could be easily differentiated from vaccine strain. it was suggested that the epidemic of poliomyelitis in 1982 was not caused by vaccine strain. the 24 isolates reacted with lsc and/or sab ... | 1987 | 3036431 |
| ionizing and ultraviolet radiation-induced reversion of sequenced frameshift mutations in escherichia coli: a new role for umudc suggested by delayed photoreactivation. | the ultraviolet (uv) and gamma radiation-induced reversion of the trpa21, trpa9813, and trpe9777 sequenced-frameshift mutations were studied in escherichia coli k-12 with or without the plasmid pkm101. radiation induced the reversion of all 3 frameshifts, and pkm101 enhanced this reversion 10-50-fold. factors influencing the differential radiation revertability of frameshifts are discussed. the two most revertable frameshifts, trpe9777 and trpa9813, were used as probes to understand the role of ... | 1987 | 3037365 |
| clustering of mutations blocking synthesis of xanthan gum by xanthomonas campestris. | mutations that block the synthesis of xanthan gum by xanthomonas campestris b1459s-4l-ii were isolated as nonmucoid colonies after treatment with ethyl methanesulfonate. complete libraries of dna fragments from wild-type x. campestris were cloned into escherichia coli by using a broad-host-range cosmid vector and then transferred into each mutant strain by conjugal mating. cloned fragments that restored xanthan gum synthesis (xgs+; mucoidy) were compared according to restriction pattern, dna seq ... | 1987 | 3038845 |
| size variation in group a streptococcal m protein is generated by homologous recombination between intragenic repeats. | m protein, a major surface protein and virulence factor for the group a streptococcus, exhibits extraordinary size variation in strains of the same serotype (fischetti et al. 1985). rna sequence analysis of spontaneous m protein size variants shows that deletion mutations arise in a single strain by homologous recombination events between intragenic tandem repeats. similar deletion and duplication events also occur in serial streptococcal isolates from a single patient and among related strains ... | 1987 | 3039291 |
| plantar fasciitis. mechanics and pathomechanics of treatment. | an excessive amount and/or a prolonged duration of pronation is the most common mechanical cause of structural strain resulting in plantar fasciitis. temporary relief of pain can be achieved by customary antiinflammatory drugs or therapy; long-term relief is achieved by adequate remedy of the aggravating pronation factors. a semirigid, custom-molded orthosis reduces excessive plantar fascial strain by supporting the first metatarsal bone and by controlling calcaneal position when in conjunction ... | 1988 | 3044618 |
| isolation and characterization of a penicillinase from pseudomonas cepacia 249. | pseudomonas cepacia has an inducible beta-lactamase which is responsible for its novel ability to catabolize beta-lactam compounds. the gene encoding this enzyme, pena, was cloned from a genomic library of p. cepacia 249 on the broad-host-range cosmid plafr. this separated the pena gene from the gene encoding a second beta-lactamase in p. cepacia 249. expression of pena was inducible in an escherichia coli host strain by low levels of penicillin. the 33,500-molecular-weight enzyme had penicillin ... | 1988 | 3046483 |
| genetic manipulation of non-conventional yeasts by conventional and non-conventional methods. | in recent years, yeasts other than those belonging to the species saccharomyces cerevisiae and schizosaccharomyces pombe have become increasingly important in industrial processes. species such as pichia stipitis, hansenula polymorpha, zygosaccharomyces rouxii, saccharomyces exiguus, torulaspora delbrueckii, yarrowia lipolytica and others whose perfect stage is known, can be manipulated genetically by classical methods, but those belonging to the genera candida (c. utilis, c. tropicalis, c. bomb ... | 1988 | 3068351 |
| plasma membrane associated antigens of trophozoites of axenic giardia lamblia. | plasma membrane isolated from axenically grown giardia lamblia (portland 1 strain) by treatment with a membrane stabilizing agent followed by osmotic stress and manual sheer showed a significant enrichment of the membrane bound marker enzymes ca++atpase/mg++atpase. crossed immunoelectrophoresis (cie) was used to identify membrane antigens. 11 major peaks were detected in cie profiles of the whole trophozoites. 6 major peaks were detected in cie profiles of the membrane preparation. 2 surface mem ... | 1988 | 3068858 |
| fecal bile acids and neutral sterols in rats with spontaneous colon cancer. | fecal bile acids and neutral sterols of spontaneous colon cancer-bearing wistar-furth strain rats were examined and compared with those of control rats of the same strain by gas chromatography and gas chromatography-mass spectrometry. the amount of total fecal bile acids and the percentage of fecal lithocholic acid were almost similar in both groups, but the percentage of fecal deoxycholic acid was significantly higher in the colon cancer group than in the controls. the amount of total fecal neu ... | 1986 | 3082773 |
| an epidemic spread of multiresistant pseudomonas aeruginosa in a cystic fibrosis centre. | early in 1983 an epidemic of a pseudomonas aeruginosa resistant to aminoglycosides, carbenicillin, ureidopenicillins, ceftazidime, cefsulodin and imipenem occurred in a cystic fibrosis centre. most of the epidemic could be attributed to a specific nosocomial strain by means of o-grouping and phage-typing. this strain was present in the centre at a low frequency in 1973 and developed resistance during courses of chemotherapy. the epidemic was stopped by isolating patients with the resistant strai ... | 1986 | 3086274 |
| genetic analysis of myxococcus xanthus and isolation of gene replacements after transduction under conditions of limited homology. | genetic analysis of myxococcus xanthus is greatly facilitated by the ability to introduce cloned dna into m. xanthus to generate gene replacement and merodiploid strains. however, gene replacement strains are difficult to obtain when the region(s) of homology between the cloned dna and the m. xanthus chromosome is limited (less than 1 kilobase). we found that gene replacements can be obtained at an increased frequency by a two-step procedure involving the use of bacteriophage p1 to isolate merod ... | 1986 | 3090023 |
| relationship between sporulation and synthesis of mycobacillin and dipicolinic acid under condition of catabolite repression in bacillus subtilis. | sporulation was repressed in the parent strain by various carbon sources whereas glucose-resistant mutants were resistant to them but not to glycerol 2-phosphate. both mycobacillin and dipicolinic acid synthesis were repressed in the parent by some of the compounds tested, viz. glucose, pyruvate and glycerol 2-phosphate. however, these syntheses in the glucose-resistant mutants were not repressed by glucose and pyruvate but were repressed by glycerol 2-phosphate. the possible interrelationship b ... | 1986 | 3093340 |
| the relationship between immunodiffusion and agglutination serotypes of mycobacterium avium and mycobacterium intracellulare. | twenty mycobacterium avium/intracellulare strains of diverse origins and two reference strains of each of schaefer's serotypes 1-21 were examined by immunodiffusion analysis. the results produced three distinct groups, which we have called a, b and c. two of these, a and b, appear to be variants, perhaps subspecies of m. avium, and group c is m. intracellulare. within each group, rough variants, untypable by schaefer's agglutination method, were found to be antigenically identical to the smooth ... | 1986 | 3096757 |
| translational autoregulation of ermc 23s rrna methyltransferase expression in bacillus subtilis. | ermc specifies an rrna methyltransferase that confers resistance to erythromycin. the expression of this determinant is induced by the addition of erythromycin. the induction mechanism has been shown to operate posttranscriptionally, and its mechanism has been elucidated. we now show that synthesis of the ermc gene product in bacillus subtilis is also autoregulated by a mechanism operating on the level of translation. the synthesis of methyltransferase was shown to be gene dosage compensated by ... | 1986 | 3096970 |
| abnormalities in hypothalamic and neurohypophysial vasopressin content are not a consequence of hypertension in the spontaneously hypertensive rat. | in order to determine if the decreased hypothalamic and increased posterior pituitary content of vasopressin (vp) observed previously in spontaneously hypertensive rats (shr) were a secondary consequence of the hypertension, the effect of preventing the development of hypertension on vp content of the hypothalamoneurohypophyseal system was evaluated. two methods for preventing the hypertension were used: (1) chronic angiotensin-converting enzyme inhibition (oral captopril, 100 mg/kg/day at 4-12 ... | 1988 | 3130152 |
| biosynthesis of branched-chain fatty acids in bacillus subtilis. a decarboxylase is essential for branched-chain fatty acid synthetase. | branched long-chain fatty acids of the iso and anteiso series are synthesized in many bacteria from the branched-chain alpha-keto acids of valine, leucine, and isoleucine after their decarboxylation followed by chain elongation. two distinct branched-chain alpha-keto acid (bcka) and pyruvate decarboxylases, which are considered to be responsible for primer synthesis, were detected in, and purified in homogenous form from bacillus subtilis 168 strain by procedures including ammonium sulfate fract ... | 1988 | 3142877 |
| isolation of colonial variants of bacteroides gingivalis w50 with a reduced virulence. | the spontaneous appearance of unusual colony forms was observed during prolonged growth of bacteroides gingivalis w50 in a chemostat. two variants were selected for further study which could be distinguished from the parent strain by the rate and intensity of pigmentation of their colonies. for example, after anaerobic incubation for 14 days, variant w50/br1 produced brown colonies whereas those of the parent strain were black; in contrast, variant w50/be1 did not show signs of pigmentation unti ... | 1988 | 3172171 |
| atpenins, new antifungal antibiotics produced by penicillium sp. production, isolation, physico-chemical and biological properties. | penicillium sp. fo-125, a soil isolate, was found to produce a new antifungal antibiotic complex named atpenin. three components a4, a5 and b were isolated from the fermentation broth of the producing strain by solvent extraction, silica gel column chromatography and hplc. the molecular formula of atpenins a4, a5 and b were determined to be c15h22no5cl, c15h21no5cl2 and c15h23no5, respectively, on the basis of high resolution electron impact mass spectrometry and elemental analysis. they are act ... | 1988 | 3209470 |
| regulation of alkaline phosphatase expression in a neonatal rat clonal calvarial cell strain by retinoic acid. | a clonal cell strain, umr 201, was established from a culture of rat calvarial cells by the process of limiting dilution on a collagen substratum. one-day-old neonatal rat calvaria stripped of periosteum were placed on collagen in alpha-mem with 10% fetal bovine serum (fbs). cells that grew out from the calvaria were passaged eight times to select cells with the ability to proliferate in culture before cloning was attempted. cells from the clonal strain were homogeneous in appearance with a doub ... | 1988 | 3213604 |
| [changes in the host-dependent characteristics of the tick-borne encephalitis virus during its adaptation to ticks and its readaptation to white mice]. | features of multiplication in pig embryo kidney (pek) cells of a variant of tick-borne encephalitis (tbe) virus previously selected by passages in h. plumbeum ticks and changes in the properties of the variant upon its repassages in white mouse brains were studied. the tick-adapted tbe variant differed from the original strain in the following characteristics: a lower yield of infectious virus and physical virus particles, altered time-course of infectious virus release from the cell, the lack o ... | 1988 | 3218215 |
| experimental mumps virus-induced labyrinthitis. immunohistochemical and ultrastructural studies. | guinea pigs were inoculated with mumps virus (torii strain) by the intralabyrinthine or intravascular route and their cochleas were examined by immunofluorescence and electron microscopy. in these animals, viral antigen was detected in the cochleas, most often in the stria vascularis. however, viral infection was produced only in those with intralabyrinthine inoculation. the cochlear lesion commonly observed in this study was severe degeneration of the organ of corti, which was usually found in ... | 1988 | 3227838 |
| [effect of gonadectomy on the onset of diabetic syndrome in the female wbn/kob rats]. | almost all the male animals of wbn/kob rat strain show the diabetic syndrome whereas none of the female animals develop such diseased conditions even at elder age. we investigated the effect of sex hormones on the onset of diabetic syndrome of this rat strain by comparing the results of body weight gain and various clinical tests such as glucose tolerance, serum biochemistry and histopathology of spayed female rats with those of non-treated and sham-operated female animals kept until 88-week-old ... | 1988 | 3240781 |
| [the influence of long-term utilization of some herbicides on oral cavity tissues of white rats]. | three mixtures of herbicides have been administered to white rats of wistar strain by means of stomach tube. after 9 months of studies laboratory examinations were carried out in these animals namely analysis of urine, peripheral blood, bone marrow smears and segments of parotid glands and cheek's mucous membrane were uptaken to histopathological examinations. morphological changes whose intensification didn't depend on the dose and type of preparation have been found in the glands. these change ... | 1988 | 3270601 |
| [genetic effects of the decay of radionuclide products of nuclear fission in saccharomyces cerevisiae cells. lethal effects of the decay of 89sr incorporated in cells of different ploidies and radiosensitivity]. | decay of 89sr incorporated in yeast cells produces a pronounced inactivating effect. the transmutation mainly contributes (about 80%) to cell inactivation. haploid cells are more sensitive to 89sr disintegration than diploid and tetraploid ones. a radiosensitive mutant xrs2, that is particularly sensitive to the transmutation effect of radionuclides, has proved to be sensitive to 89sr transmutation as well. at the same time, another radiosensitive mutant, rad 54, does not virtually differ from t ... | 1988 | 3278334 |
| legionella cincinnatiensis sp. nov. isolated from a patient with pneumonia. | a legionella-like organism (strain 72-oh-h [= atcc 43753]) was isolated from an open-lung biopsy specimen from a hemodialysis patient with end-stage renal disease and bronchopneumonia. growth characteristics and gas-liquid chromatographic profiles of the isolate were consistent with those for legionella spp. the isolate was presumptively identified as a legionella longbeachae serogroup 1 strain by direct immunofluorescence staining. however, the organism was serologically distinct in the slide a ... | 1988 | 3281971 |
| genetic and phenotypic analyses indicating occurrence of the recn262 and radb101 mutations at the same locus in escherichia coli. | the radb101 and recn262 mutations showed essentially identical phenotypes when compared in isogenic escherichia coli strains for their effects on gamma and uv radiation survival and on conjugal recombination in a uvra recb recc sbcb sbcc strain. complementation tests involving attempts to reconstitute a radb+ recn+ strain by transductions between radb101 and recn262 donors and recipients, and tests involving plasmids carrying recn+ and recn::tn1000 inserts, indicated that the radb and recn genes ... | 1988 | 3283110 |
| non-h-2 histocompatibility antigens encoded by moloney-murine leukemia virus in mov mouse strains are detectable by skin grafting and cytolytic t lymphocytes. | the integration and expression of moloney-murine leukemia virus (m-mulv) into the germ line of mov mouse strains on the c57bl/6 background results in the expression of a cell-surface ag with characteristics expected from non-h-2 histocompatibility ag: the ability to stimulate graft rejection and generation of ctl. however, both the previously studied mov-3 and mov-14 strains differ from the coisogenic c57bl/6 strain by different length segments of chromosome derived from the icr strain in additi ... | 1988 | 3286772 |
| [clinical aspects and problems of pigeon breast and funnel chest]. | chest wall protrusion and depression deformities, also known as funnel chest and pigeon chest, are nosologically a uniform entity. our own histopathological studies revealed secondary changes, found likewise in arthroses, scolioses, aseptic osteonecroses and inflammatory processes, as well as changes found in so-called primary collagenous diseases of unknown aetiology. investigation of the collagen metabolism did not disclose differences from the normal ii-type collagen either qualitatively or q ... | 1988 | 3287794 |
| [aminoside sensitivity of bacteria isolated in 1984 at the military hospital complex in the paris region]. | the minimal inhibiting concentrations (mic) of 5 aminosids have been determined by the microdilution method in liquid medium of 4,582 bacterial strains isolated from various pathological samples: 1,039 staphylococcus, 2,629 enterobacteria, 759 pseudomonas and 155 acinetobacter. the phenotype of resistance has been defined for each strain by listing the antibiotics for which a resistance was observed. the frequencies of the bacterial resistances varied according to the aminosid (gentamicin, sisom ... | 1987 | 3302847 |
| cross-reactive antibody in immunity to colisepticemia in calves. | cattle were immunized with a uridine diphosphate galactose epimerase deficient mutant of escherichia coli to prepare antiserum cross-reactive with different serotypes of e. coli. hypogammaglobulinemic calves were given bovine anti-j5 serum before oral challenge with virulent e. coli derived from a septicemic calf. passively immunized calves had delayed and decreased bacteremia compared with calves given saline before challenge. calves given antiserum also lived longer than control calves. a seco ... | 1987 | 3310376 |
| stress induced periosteal changes. | the tails of sprague-dawley rats of approximately 50 g body mass were either left straight or bent to form a loop containing three or five vertebrae (cv). subsequent treatment was as follows: (a) in situ: segments were removed at 0, 8, 16, 24 h, 3, 5 and 7 days and examined histologically: (b) transplants: skinned segments were transplanted autologously and examined at the same time intervals; (c) normal saline: skinned segments were placed in a 0.9% solution maintained at (i) 4 degrees c and (i ... | 1987 | 3322355 |
| a gale via (vi antigen-negative) mutant of salmonella typhi ty2 retains virulence in humans. | we have recently described the construction of a gale derivative of salmonella typhi ty2 (ty2h1) which had a 0.4-kilobase deletion in the gale gene and was sensitive to galactose-induced lysis when cultured with greater than or equal to 0.06 mm galactose (d. m. hone, r. morona, s. attridge, and j. hackett, j. infect. dis. 156:167-174, 1987). we now report the selection of a rifampin-resistant, via derivative of ty2h1, ex462. compared with the ty2 parent strain, ex462 was serum sensitive and high ... | 1988 | 3356467 |
| characterization of resistance phenotype and cephalosporin activity in oxacillin-resistant staphylococcus aureus. | forty isolates of methicillin-resistant staphylococcus aureus were tested versus oxacillin at 30 and 35 degrees c with and without 2% nacl supplementation of mueller-hinton broth and classified as having resistance that was low (mic, less than or equal to 16 micrograms/ml) or high (mic, greater than or equal to 32 micrograms/ml) and temperature or nacl dependent. only three isolates had low-grade resistance at both 30 and 35 degrees c; for two isolates the mics at 35 degrees c were greater than ... | 1988 | 3364941 |
| studies on the resistance of clostridium difficile to antimicrobial agents. | the susceptibility of c. difficile isolated at the department of medical microbiology of the university of zurich to a wide selection of antibacterial, antimycobacterial and antifungal agents was tested in vitro. great differences in susceptibility were found against chloramphenicol, clindamycin, erythromycin, rifamycin, and tetracycline. resistance to clindamycin and erythromycin could always be transferred jointly to a susceptible c. difficile strain by mixed culture on filters at low frequenc ... | 1988 | 3376619 |
| creation of a strain of genetically obese-hypertensive rats. | a strain of genetically obese-hypertensive rats (shr-fa/fa) was created by transferring the fatty/fa gene of hyperlipaemic obese non-inbred rats into the genome of an shr inbred strain by five successive crossings of shr-fa+ brother-sister matings. shr-fa/fa rats were heavier than their shr littermates. they showed a severe hypertension, their systolic arterial blood pressure being higher than that previously found in genetically hypertensive rats. their blood glucose content was not significant ... | 1988 | 3384559 |
| metabolic evidence for the involvement of enzymatic bioactivation in the cataractogenicity of acetaminophen in genetically susceptible (c57bl/6) and resistant (dba/2) murine strains. | acetaminophen has been shown to be cataractogenic in mice and rabbits. c57bl/6 and dba/2 mice respectively are genetically susceptible and resistant to the induction of cytochrome p-448 by 3-methylcholanthrene (3-mc). this isoenzyme is thought to bioactivate acetaminophen to a toxic reactive intermediate. these two murine strains also are correspondingly susceptible and resistant to acetaminophen cataractogenesis. to evaluate the potential role of enzymatic bioactivation as a determinant of acet ... | 1988 | 3400097 |
| synthesis and biological activity of some further cyclopenta[a]phenanthrenes. | 16,17-dihydro-11-methoxy-15h-cyclopenta[a]phenanthrene was synthesized by two different routes and tested for carcinogenicity in tylers original (to) and sencar strain of mice by repeated application and initiation/promotion regime. 16,17-dihydro-11-hydroxy-15h-cyclopenta[alpha]phenanthrene was also tested for carcinogenicity in the to strain by repeated application. surprisingly with both of these compounds, which lack conjugation in ring d, one of the ingredients essential for biological activ ... | 1988 | 3409471 |
| organization and characterization of genes essential for symbiotic nitrogen fixation from bradyrhizobium japonicum i110. | a total of 96 independent tn5 insertions within a 39-kilobase-pair (kbp) segment of chromosomal dna containing the three structural genes for nitrogenase (nifh, nifd, and nifk) from bradyhizobium japonicum i110 were obtained in escherichia coli and transferred to the wild-type strain by marker exchange. individual transconjugants containing a tn5 insertion were inoculated onto glycine max cv. wilkin (soybeans) and analyzed for their effect on symbiotic nitrogen fixation. in addition to the three ... | 1986 | 3462181 |
| fractionation and characterization of two beta-lactamases in citrobacter diversus ula-27 strain by chromatofocusing. | 1987 | 3500180 | |
| the alloantibody response in the allogeneically pregnant rat. v. absence of cell-mediated immunity in high responders. | allogeneically pregnant rats have been examined postpartum to determine whether they are sensitized against paternal class i antigens for cell-mediated immunity. this study was undertaken as this point is ambiguous. since the response of the female to paternal mhc antigens is genetically controlled it is possible that the inability of some investigations to detect cell-mediated immunity against the paternal strain was due to the use of non-responder strain combinations. cell-mediated immunity wa ... | 1987 | 3500309 |
| an altered ribosomal protein in an edeine-resistant mutant of saccharomyces cerevisiae. | the r-proteins of an edeine-resistant mutant of saccharomyces cerevisiae were compared to those of the wild-type strain by using two different two-dimensional electrophoretic techniques: (1) the kaltschmidt-wittmann method and, (2) the kaltschmidt-wittmann system, in the first dimension and the na dodecyl-so4 system in the second. with the first technique, the results indicate that the patterns of basic ribosomal proteins are similar in the two strains. however, the pattern of acidic ribosomal p ... | 1986 | 3515127 |
| penicillin tolerant group a streptococci. | a penicillin (pcn) tolerant [minimal inhibitory concentration (mic) less than or equal to 0.02, minimal bactericidal concentration (mbc) = 3.10 micrograms/ml] group a streptococcus (gas) was recovered from the bone aspirate of a child with osteomyelitis. the penicillin therapy with 200,000 micron/kg x day, and subsequently ampicillin 360 mg/kg x day, resulting in a serum ampicillin concentration of 74 micrograms/ml, failed to achieve a serum bactericidal effect greater than 1:2. ninety-nine addi ... | 1986 | 3516551 |
| [experimental hyperlipoproteinemia and arteriosclerosis in minipigs--effect of various drugs]. | experimental hyperlipoproteinemia and atherosclerosis were produced in mini-pigs of the göttingen strain by adding egg yolk and cholesterol to the diet for one and a half year. phenotyping of the hyperlipoproteinemia showed a great similarity with the human type iia according to the fredrickson classification system. cholesterol ester-rich atherosclerotic lesions were developed, predominantly located in the abdominal aorta and the coronary arteries. prophylactic treatment of the hyperlipoprotein ... | 1986 | 3532591 |
| isolation of a mycelial mutant of candida albicans. | a mutant of candida albicans strain men, which was unable to produce mycelia in ssv medium and in horse serum at 37 degrees c, was isolated by a physical separation procedure. the mutant was shown to be derived from the parental strain by growth and morphology studies, sugar uptake and fermentation patterns, and the presence of genetic markers. | 1986 | 3540202 |
| characterization of phosphomannan-protein complexes isolated from viable cells of yeast and mycelial forms of candida albicans nih b-792 strain by the action of zymolyase-100t. | the isolation of phosphomannan-protein complexes from the viable cells of yeast (y) and mycelial (m) forms of candida albicans nih b-792 strain was conducted by treatment with zymolyase-100t followed by fractional precipitation with cetyltrimethylammonium bromide. the m-form complex was found to contain smaller amount of phosphate (1.3%) than that of the y-form complex (1.6%). proton magnetic resonance (pmr) spectra of these complexes indicated that the content of beta-1,2-linked oligomannosyl a ... | 1986 | 3541789 |
| structure-activity relationship of nitroimidazo (2,1-b) thiazoles in the salmonella mutagenicity assay. | the mutagenic activity of a series of nitroimidazo (2,1-b) thiazoles was determined in salmonella typhimurium ta-100 strain by means of the ames test. a multiple regression analysis showed a highly significant parabolic relationship between mutagenic activity and the rm values as an expression of the lipophilic character of molecules. the lipophilic character should be important in determining an optimal cell permeation. however when the lipophilic character was expressed by means of the sigma p ... | 1986 | 3545148 |
| efficacy of commercial mycoplasma gallisepticum bacterin (mg-bac) in preventing air-sac lesions in chickens. | one-week-old chickens were vaccinated with commercial mycoplasma gallisepticum bacterin subcutaneously and challenged with the s6 strain by the intra-air-sac route 3 weeks later. significantly fewer vaccinated chickens had air-sac lesions than controls. | 1987 | 3579788 |
| the phosphoenolpyruvate: sugar phosphotransferase system of streptococcus salivarius. identification of a iiiman protein. | a double-spontaneous mutant resistant to the growth inhibitory effect of alpha-methylglucoside and 2-deoxyglucose was isolated from streptococcus salivarius. this mutant strain, called alpha s3l11, did not grow on mannose and grew poorly on 5 mm fructose and 5 mm glucose. isolated membranes of strain alpha s3l11 were unable to catalyse the phosphoenolpyruvate-dependent phosphorylation of mannose in the presence of purified enzyme i and hpr. addition of dialysed membrane-free cellular extract of ... | 1987 | 3580966 |
| dietary fat in relation to hormonal induction of mammary and prostatic carcinoma in nb rats. | mammary carcinoma was induced in female nb rats by implanting pellets of estrone at 25-28 days of age, and prostatic cancer was induced in male rats of the same strain by repeatedly implanting pellets of testosterone propionate. feeding semipurified diets containing either 3% or 20% corn oil did not significantly affect the incidence or yield of mammary tumors, although tumors tended to appear somewhat earlier in animals on the high-fat diet, confirming the report of dunning et al. published nea ... | 1987 | 3608084 |
| age dependent change in size distribution of blood cells in fetal and young mice. | the mean corpuscular volume (mcv) and the size distribution of circulating blood cells were determined in the fetal mice of c3h/he strain by using a new electric cell size analyser, coulter channelyzer c-256. on the 12th day of gestation, the volume of circulating blood cells was distributed between approximately 240-820 fl (mode at 470 fl), and the mcv was 534.9 +/- 30.1 fl, i. e., ten to eleven times that of adult one. on the 14th day, two types of cell population were observed; one with small ... | 1987 | 3609153 |
| proximity storage of surgical instruments in the operative field. | a new microsurgical instrument, the surgical pocket, is described, and its value for storing instruments in the surgical field during operation is discussed. specific procedures for using the pocket in the field with the bipolar coagulator, suction irrigator, dissector, and scissors are presented. proximity storage of microsurgical instruments reduces the surgeon's reliance upon other surgical personnel by allowing instrument transfers to be made without assistance. in addition, it reduces stres ... | 1987 | 3614576 |
| [immunomodulation with natural products. i. effect of an aqueous extract of raphanus sativus niger on experimental influenza infection in mice]. | a raphanus sativus niger water extract was administered by intranasal instillations to mice before inoculation of the influenza virus a/pr 8/34 (h1n1) strain by the same route. the extract ensured some protection against the experimental influenza infection. a significant decrease of the hemagglutinin titre of the mouse lung homogenate was noted, as well as a decrease of the mortality rate and a significant increase of the rate of survival as compared to the untreated controls. | 1987 | 3617495 |
| [comparative characteristics of the diagnostic properties of staphylococcal allergens in an experiment]. | the diagnostic value of five staphylococcal allergens prepared from a single s. aureus strain by different methods and in different institutions has been tested on the experimental models of delayed, immediate and mixed (immediate and delayed) hypersensitivity in guinea pigs. the advantages of the allergens prepared in kazan (ussr) for the detection of delayed hypersensitivity and the ultrasonicated allergen, as well as the allergen made in czechoslovakia, for the detection of immediate hypersen ... | 1987 | 3618026 |
| efficacy of ciprofloxacin for experimental endocarditis caused by methicillin-susceptible or -resistant strains of staphylococcus aureus. | the efficacy of ciprofloxacin for experimental aortic valve endocarditis in rabbits infected by either a methicillin-susceptible or a methicillin-resistant strain of staphylococcus aureus was compared with standard therapy of nafcillin or vancomycin, respectively. after 4 days of therapy, ciprofloxacin reduced the counts of organisms in aortic valve vegetations as effectively as the standard regimen for both susceptible and resistant strains. mean concentrations of ciprofloxacin in serum achieve ... | 1986 | 3640590 |
| differences in the acquisition process and the effect of scopolamine on radial maze performance in three strains of rats. | the acquisition process and the effect of scopolamine (scop) on the radial maze task were studied in 3 strains of male rats, fischer 344 (f344), sprague-dawley (sd) and wistar. the pretraining level of locomotor activity was measured and performance was quantitatively and qualitatively evaluated. the highest pretraining locomotor activity was observed in wistar rats. in this experiment, rats were allowed to select each arm successively. the changes in the number of correct choices during the fir ... | 1987 | 3659071 |
| age-related natural antibody specificities among hybridoma clones originating from nzb spleen. | in the studies presented here, age-related natural antibody specificities have been investigated in the autoimmune nzb mouse strain by cell fusion. the monoclonal immunoglobulins (mig) secreted by productive hybridoma clones were examined for their antibody activities against a panel of antigens, including single- and double-stranded dna, actin, tubulin, myosin, bromelain-treated mouse red blood cells (brmrbc) and tnp-bsa, employing both direct and competitive enzyme immunoassays. the antibody s ... | 1987 | 3666786 |
| [evaluation of the virulence of a staphylococcus strain by the phagocytic activity of polymorphonuclear leukocytes]. | 1987 | 3691306 | |
| establishment and growth kinetics of the mutant ehrlich-lettré ascites cell strain hd33 in permanent suspension culture. | cells of a mutant in vivo subline of the ehrlich-lettré mouse ascites tumour (elat) were converted to growth in suspension culture. kinetic analysis revealed the selective character of the conversion process; without a detectable adaptation period, a fraction of about 2 x 10(-5) of the explanted cells continued to grow in vitro. the resulting, mutant ehrlich-lettré ascites cell strain was designated hd33 and propagated uninterruptedly from 1974 on. the corresponding in vivo elat subline hd33 was ... | 1986 | 3719659 |
| eimeria tenella: immunogenicity of the first generation of schizogony. | the life-cycle of a precocious and attenuated line (wisf96) of eimeria tenella, derived from the wisconsin (wis) strain, contained only the first of the three generations of schizogony undergone by the parent strain. the reproductive capacity of wisf96 was less than that of the parent strain by a factor of about 2000, but inoculation of chickens on two occasions with a large number of its oocysts induced resistance against challenge with oocysts, or with second-generation merozoites of wis. the ... | 1986 | 3748607 |
| chronic respiratory disease in premature infants caused by chlamydia trachomatis. | the relation between chronic respiratory disease and infection with chlamydia trachomatis in premature infants was investigated to ascertain the aetiological importance of intrauterine c trachomatis infection and chronic respiratory disease in premature infants. serum igm antibodies against c trachomatis were determined by enzyme linked fluorescence assay. sections of lung tissues obtained by biopsy and at necropsy were also tested for the presence of antigens using fluorescein conjugated monocl ... | 1986 | 3753987 |
| acetolysis of pichia pastoris ifo 0948 strain mannan containing alpha-1,2 and beta-1,2 linkages using acetolysis medium of low sulfuric acid concentration. | to obtain manno-oligosaccharides containing beta-1,2-linked nonreducing terminal groups from the mannan of pichia pastoris ifo 0948 strain by acetolysis, an attempt was made to establish the reaction conditions under which cleavage of the alpha-1,6 linkage took place preferentially leaving manno-oligosaccharides composed largely of beta-1,2 linkages. by the action of an ordinary acetolysis medium, a 10/10/1 (v/v) mixture of acetic anhydride, acetic acid, and sulfuric acid at 40 degrees c for 13 ... | 1986 | 3754107 |
| erythrocyte protein conjugates as a principle of biological monitoring for pesticides. | the determination of erythrocyte protein conjugates is recommended for monitoring worker's exposure to reactive pesticides or group of pesticides. under the assumption that reversible and especially irreversible toxic effects of these substances occur in parallel to the formation of erythrocyte adducts, the determination of the amount of adduct can be seen as a parameter for monitoring stress and strain by these substances. the application of this method is demonstrated in some practical example ... | 1986 | 3775814 |
| [accommodation and visual evoked potential during eye strain by experimental visual load]. | 1986 | 3799377 | |
| effects of comb dubbing on the performance of laying stocks. | three studies were conducted with birds dubbed at hatch vs. dubbed and dewattled at 118 days or 255 to 260 days of age or with normal hens. in the first, involving 5928 pullets of four strains housed 1 per cage, dubbing and dewattling at 255 to 260 days caused small adverse effects on egg numbers as compared with nondubbed controls. body size was reduced, and both egg weight (ew) and shell strength were increased slightly. in the second study, involving 8180 pullets of nine strains housed 1 per ... | 1985 | 3838812 |
| computational statistics for cell survival curves. i. evaluation of the colony-forming ability of a single cell strain by the apl function cfaline. | an interactive program for the statistical analysis of cell survival curves by a linear regression approach is described. it allows for the computational determination of the linear part of a cell survival curve and the determination of a common slope estimate in the presence of repeated experiments for a particular cell strain with the appropriate variance estimates and confidence intervals. | 1985 | 3852713 |
| chromosomal deletion and plasmid complementation of the photosynthetic reaction center and light-harvesting genes from rhodopseudomonas capsulata. | using in vitro interposon mutagenesis, rhodopseudomonas capsulata strains have been constructed wherein all or part of the reaction center (rc), light-harvesting i (lhi), and light-harvesting ii (lhii) structural genes have been deleted. in one series of strains, the 2778-bp apai fragment bearing more than 90% of the rxca operon (promoter and structural genes coding for lhi beta, lhi alpha, rc-l and rc-m) has been deleted from the chromosome. when the rxca operon is deleted, resultant strains po ... | 1985 | 3864717 |
| idiotypic analysis of polyclonal and monoclonal anti-p-azophenylarsonate antibodies of balb/c mice expressing the major cross-reactive idiotype of the a/j strain. | the idiotypic cascade allows the induction of silent idiotypes, and as such, the immune system can be reprogrammed towards predetermined goals. to understand the genetic origin of silent idiotypes, we have used a system in which detailed structural and genetic information is available. the major cross-reactive idiotype (cria) of a/j mice (positive strain) immunized with arsonate coupled to a carrier can be regularly induced in balb/c mice (negative strain) by anti-idiotypic treatment with or wit ... | 1985 | 3871458 |
| strain differences in brain biogenic amines concentration in rats with different resistance to emotional stress. | blood pressure and heart rate were measured under the conditions of prolonged emotional stress due to 30 h immobilization in rats of various strain: 1. wistar; 2. veg (deviated from wistar); 3. august; 4. bastards. some defined areas of brain from wistar and august rats were dissected and used for the estimation of noradrenaline (na) and dopamine (da) content with the aid of spectrofluorimetric method. it was found that, among the animals of all strains used, different groups may be defined acco ... | 1985 | 3872789 |
| immune abnormalities in the diabetic new zealand obese (nzo) mouse: insulin treatment partially suppresses splenic hyperactivity measured by flow cytometric analysis. | immune abnormalities in both the humoral and cell-mediated immune systems were found in new zealand obese (nzo) mice, a model of diabetes associated with autoimmune disease. both the formation of anti-sheep red blood cell plaques and mitogen stimulation of nzo spleen cells were significantly decreased compared to normal strains of mice. in addition to perturbations of splenic lymphocyte function, the enlarged spleens of nzo mice demonstrated increased cycling cells as measured by flow cytometric ... | 1985 | 3891173 |
| effect of extracellular protease production on bacteriophage sensitivity of vibrio cholerae. | the effect of varying levels of extracellular protease production on the bacteriophage type of vibrio cholerae 1621 serotype 01, biotype e1 tor, has been investigated. it has been shown that the production of high levels of exoprotease can alter the apparent type of the strain by rendering it insensitive to infection by a number of bacteriophages. prevention of a productive infection appears to be due to altered surface characteristics rather than to specific damage to the bacteriophages. such a ... | 1985 | 3913071 |
| bony mass associated with hypertrophy of the sternohyoid muscle after radical neck dissection. | as a late complication after radical neck dissection, this paper reports a bony mass at the sternal end of the clavicle associated with hypertrophy of the sternohyoid muscle. the bony mass and hypertrophy of the muscle were considered due to continuous mechanical strain by the drooping of the shoulder caused by injury of the accessory and cervical nerves, left-handed physical labour, and the lack of the sternocleidomastoid muscle in rotating the head. | 1985 | 3921485 |
| [transfer and expression of mutations in pleiotropic genes determining the synthesis of extracellular enzymes in bacillus subtilis]. | the mutations in the genes determining the levels of extracellular enzymes production were transferred into bacillus subtilis 168 strain by genetic transformation technique. the method used has permitted registering the transfer of pleiotropic genes. seven amylase producers were detected among 126 his+ transformants screened, as well as five metalprotease producers among 246 gly+ transformants. cotransfer of pap and hpr mutant genes linked with his+ or gly+ genes might result in finding the prod ... | 1985 | 3939574 |
| survival and growth of yersinia pestis within macrophages and an effect of the loss of the 47-megadalton plasmid on growth in macrophages. | the survival and growth of yersinia pestis cells within mouse peritoneal cavities and within mouse peritoneal macrophages maintained in vitro was examined. two strains were used which differed only in that one (kim) contained the 47-megadalton plasmid associated with virulence and the second (kim1) lacked this plasmid. the kim cells, but not the kim1 cells, acquired some resistance to phagocytosis during growth at 37 degrees c which was not evident when cells were grown at 26 degrees c. whether ... | 1985 | 3965398 |
| characterization of virulent and avirulent a/chicken/pennsylvania/83 influenza a viruses: potential role of defective interfering rnas in nature. | in april 1983, an influenza virus of low virulence appeared in chickens in pennsylvania. subsequently, in october 1983, the virus became virulent and caused high mortality in poultry. the causative agent has been identified as an influenza virus of the h5n2 serotype. the hemagglutinin is antigenically closely related to tern/south africa/61 (h5n3) and the neuraminidase is similar to that from human h2n2 strains (e.g., a/japan/305/57) and from some avian influenza virus strains (e.g., a/turkey/ma ... | 1985 | 3973976 |
| the influence of dosing schedules and cerebrospinal fluid bactericidal activity on the therapy of bacterial meningitis. | bacterial meningitis represents an infection in an area of impaired host defence. optimal therapy of meningitis requires attaining bactericidal activity within cerebrospinal fluid (csf). studies in experimental animal models of meningitis suggest that maximal rates of bacterial killing in vivo and optimal cure rates are achieved when csf antibiotic concentrations exceed the mbc of the test strain by greater than or equal to ten-fold. the results of clinical trials support this conclusion. in add ... | 1985 | 3980333 |
| cross-linking of the enzymes in the glycosome of trypanosoma brucei. | glycosomes, the microbody-like organelles containing mainly glycolytic enzymes, were purified from the long slender bloodstream form of trypanosoma brucei eatro 110 monomorphic strain by an improved method in which the protozoa were frozen and thawed in 15% glycerol to free, from the plasma membrane, much of the variant surface glycoprotein which used to constitute the major contaminant of our purified glycosomes. the purified glycosomes have 11 major proteins, 6 of which, tentatively identified ... | 1985 | 3997856 |