Publications

the rocks and shallows of deep rna sequencing: examples in the vibrio cholerae rnome.	new deep rna sequencing methodologies in transcriptome analyses identified a wealth of novel nonprotein-coding rnas (npcrnas). recently, deep sequencing was used to delineate the small npcrna transcriptome of the human pathogen vibrio cholerae and 627 novel npcrna candidates were identified. here, we report the detection of 223 npcrna candidates in v. cholerae by different cdna library construction and conventional sequencing methods. remarkably, only 39 of the candidates were common to both sur ...	2011	21610211
n-terminal residues of the vibrio cholerae virulence regulatory protein toxt involved in dimerization and modulation by fatty acids.	the regulatory protein toxt is an arac family protein that is responsible for activating transcription of the genes encoding cholera toxin and toxin coregulated pilus, which are required for virulence by the human pathogen vibrio cholerae. the n terminus of toxt contains dimerization and regulatory elements, whereas the c terminus contains the dna binding domain. bile and long chain fatty acids negatively regulate toxt activity. utilizing a comprehensive alanine substitution mutant library of to ...	2011	21673111
physical, chemical and microbiological quality of ice used to cool drinks and foods in greece and its public health implications.	ice used for direct human consumption or to preserve foods and cool down drinks can be contaminated with pathogenic microorganisms and may potentially become a vehicle for consumer's infection. to evaluate physical, chemical and microbiological quality of commercial ice and ice used for fish and seafood, 100 ice samples collected at 10 different retail points in the region of epirus were studied. the following microbiological parameters were determined: total coliforms, fecal coliforms, salmonel ...	2011	21802520
The Vibrio cholerae mannitol transporter is regulated post-transcriptionally by the MtlS small RNA.	Vibrio cholerae continues to pose a health threat in many developing nations and regions of the world struck by natural disasters. It is a pathogen that rapidly adapts to aquatic environments and the human small intestine. Small regulatory RNAs (sRNAs) may contribute to this adaptability. Specifically, the mannitol operon sRNA (MtlS; previously IGR7) is transcribed antisense to the 5' untranslated region of the mtl operon, encoding the mannitol-specific phosphotransferase system. Mannitol is a ...	2011	22101846
development of a sensitive rrna-targeted reverse transcription-quantitative polymerase chain reaction for detection of vibrio cholerae/mimicus, v. parahaemolyticus/alginolyticus and campylobacter jejuni/coli.	a sensitive rrna-targeted reverse transcription-quantitative polymerase chain reaction (rt-qpcr) method was developed for detection of vibrio cholerae/mimicus, v. parahaemolyticus/alginolyticus and campylobacter jejuni/coli by using specific primers. counts of the enteric pathogens spiked in human stools were quantified at the lower detection limit of 10(3) cells /g of stools by rt-qpcr, in marked contrast with conventional quantitative polymerase chain reaction (qpcr) at the detection limit of ...	2011	22146006