Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| dual infection of chickens with acute and mild strains of marek's disease herpesvirus. | 1973 | 4712809 | |
| differences between two lines of chickens in the response to acute and mild strains of marek's disease virus. | 1973 | 4748342 | |
| the influence of maternal effects on the response of fast and slow growing chickens to a marek's disease virus. | 1973 | 4754048 | |
| neutralization studies with marek's disease virus and turkey herpesvirus. | use of marek's disease virus (mdv) in a neutralization test presents several problems, which are described, making this potentially useful test difficult. to obviate these difficulties, a plaque reduction test has been designed based on cross-neutralization of turkey herpesvirus (hvt) by serum-neutralizing mdv. the technique for such a neutralization test is outlined. kinetics of development of neutralizing antibodies in chickens inoculated with hvt and mdv are described. the neutralization test ... | 1973 | 4767297 |
| [a study of the properties of the kekava strain of marek's disease virus in experiments on chickens]. | 1973 | 4789250 | |
| high-virulence marek's disease virus infection in chickens previously infected with low-virulence virus. | 1974 | 4831444 | |
| the in vitro and in vivo effect of chemotherapeutic agents on the marek's disease herpesvirus of chickens. | 1974 | 4854877 | |
| combined fluorescent-antibody and electron microscopy study of marek's disease virus-infected cell culture. | duck embryo fibroblast (def) and chicken embryo fibroblast (cef) cultures infected with marek's disease virus were studied by combined fluorescent antibody and electron microscopy techniques. in both def and cef cultures, cells containing immunofluorescent (if) antigen also contained herpesvirus particles; conversely, cells lacking this antigen lacked herpesvirus particles. two morphologically distinct if antigens were detected in the cytoplasm. (i) a granular antigen in the perinuclear region w ... | 1970 | 4909910 |
| efficiency of commercial air filters against marek's disease virus. | in a series of 10 replicate trials with susceptible chickens as indicators of the presence of virus in the air, filter b(1) (dri pak, american air filter co.), which has a dust spot rating of 93 to 97%, and filter c (astrocel), with a dioctyl phathalate aerosol rating of over 99%, completely removed marek's disease virus (mdv) from highly contaminated air. however, air passed through filter b(2) (duracel), with a dust spot rating of 34 to 45%, remained infectious. thus, an air filter with a dust ... | 1972 | 5021970 |
| isolation of an anaerobic mycoplasma from avian cell cultures and some of its effects on marek's disease virus. | 1972 | 5038652 | |
| evaluation of the chicken immune response to marek's disease herpesvirus. | 1971 | 5155564 | |
| effect of infectious bursal disease virus on protection against marek's disease by turkey herpesvirus vaccine. | chickens inoculated at hatch with pathogenic infectious bursal disease virus (ibdv) and the turkey herpesvirus (hvt) vaccine were poorly protected against challenge at 1 week with virulent marek's disease virus (mdv). however, if vaccination with hvt preceded ibdv inoculation such as by embryonal vaccination with hvt and exposure to ibdv at hatch, or by vaccination at hatch and ibdv exposure 5 days or longer postvaccination, then the vaccinal efficacy of hvt was not influenced. further, the inhi ... | 1984 | 6091603 |
| cell-specific antiviral activity of 1-(2-fluoro-2-deoxy-beta-d-arabinofuranosyl)-5-iodocytosine (fiac) against marek's disease herpesvirus and turkey herpesvirus. | three new fluoroarabinosylpyrimidine nucleosides (fiac, fiau and fmau) were tested for in vitro activity against oncogenic and nononcogenic strains of marek's disease virus (mdv) and herpesvirus of turkeys (hvt). marek's disease is a herpesvirus-induced lymphoma in chickens. nononcogenic strains of mdv and hvt can protect against this disease. all viruses were inhibited by 1 microm of these drugs in chick kidney cell (ckc) cultures, but only fmau and fiau were active in chicken embryo fibroblast ... | 1984 | 6097179 |
| an enzyme-linked immunosorbent assay for the detection of antibodies to marek's disease virus. | a reproducible enzyme-linked immunosorbent assay (elisa) using marek's disease virus (mdv)-infected cells for the detection of antibodies to mdv is described. the optimum number of mdv-infected chicken embryo fibroblasts (cef) was 5 x 10(4)/well, and test sera were positive at 1:400 dilutions. compared with a purified virus preparation, mdv-infected cef produced high specific and low nonspecific reactivities. wells coated with whole cells could be stored at 4 c or -20 c for at least 3 months wit ... | 1984 | 6098254 |
| antigen requirements and specificity of a microplate enzyme-lined immunosorbent assay (elisa) for marek's disease viral antibodies in chicken, mouse and rabbit sera. | a rapid indirect enzyme linked immunosorbent assay for the detection of antibodies against marek's disease virus in mouse, chicken and rabbit-sera has been established. optimal sensitivity was obtained by using 10 micrograms/ml of partially purified viral proteins. various experimental procedures performed to eliminate nonspecific reactions are described. best results were obtained by the introduction of np 40 at a concentration of 0.1% in sera. with mouse sera, the pretreatment of plaques with ... | 1984 | 6099079 |
| enzyme-linked immunosorbent assay of serum antibodies to marek's disease virus in vaccinated chickens. | 1982 | 6127941 | |
| monoclonal antibodies with specificity for three different serotypes of marek's disease viruses in chickens. | a total of 50 antibody-secreting hybridoma cells against marek's disease virus (mdv) and turkey herpesvirus (hvt) have been produced. eleven hybridomas were used for serotyping a panel of 15 pathogenic and nonpathogenic strains of mdv and hvt, representing three serotypes. the antibodies from the culture medium have fluorescence antibody (fa) titers of up to 100 and those from mouse ascitic fluid have titers ranging from 10(4) to 10(6). monoclonal antibody t81 is type-common, i.e., it reacts at ... | 1983 | 6184394 |
| specificity in the immunosuppression induced by avian reticuloendotheliosis virus. | several parameters of the cellular and humoral immune responses of chickens infected with reticuloendotheliosis virus (rev-t), an avian defective acute leukemia virus, or with its helper virus, reticuloendotheliosis-associated virus (rev-a), were evaluated. spleen cells from chickens infected with rev-t (rev-a) or rev-a exhibited depressed mixed lymphocyte and mitogen responses in vitro. allograft rejection was delayed by 6 to 14 days in birds infected with rev-a. the specific antitumor cell imm ... | 1983 | 6187691 |
| monoclonal antibody-mediated immunoprecipitation of proteins from cells infected with marek's disease virus or turkey herpesvirus. | the major immunogenic viral proteins of marek's disease virus (mdv) and turkey herpesvirus (hvt) share antigenic determinants. the polyacrylamide gel electrophoresis pattern of five viral polypeptides immunoprecipitated with homologous convalescent chicken plasma was identical with the pattern obtained after immunoprecipitation with heterologous convalescent chicken plasma. a panel of monoclonal antibodies was used to identify mdv and hvt polypeptides. sixteen monoclonal antibodies were positive ... | 1984 | 6205501 |
| the latex particle adherence assay: inhibitory effects of protein preparations. | the latex particle adherence to peripheral chicken lymphocytes in the presence of different concentrations of various protein preparations was examined. non-specific inhibition of adherence was observed at and above concentrations of 500 microgram/ml, whereas protein concentrations at and below 250 microgram/ml gave no significant adherence inhibition in control chicken lymphocytes. the protein concentration of 250 microgram/ml of antigens prepared from mdv-infected chicken skin and hprs-1 cells ... | 1980 | 6244992 |
| cell-mediated immunity in marek's disease: correlation of disease-related variables with immune responses in age-resistant chickens. | a sequential study of cytotoxic and mitogen stimulation responses of peripheral blood leukocytes (pbl) after inoculation with marek's disease (md) virus was done with the 7 x 15 chickens at 2 weeks of age (group 1) and at 12 weeks (group 3). control groups were inoculated with chicken kidney cells at 2 weeks of age (group 2) and 12 weeks (group 4). during an 8-week observation period, morbidity and mortality were greater in group 1 chickens than in group 3 chickens. the cytotoxic response of pbl ... | 1980 | 6245607 |
| effect of neonatal thymectomy on pathogenesis of herpesvirus of turkeys in chickens. | effect of immunosuppression on pathogenicity of herpesvirus of turkeys (hvt) in chickens was studied. in three experiments, newly hatched chickens of two genetic strains were surgically thymectmized and gamma-irradiated and then exposed to hvt. lymphoid cells of thymectomized-irradiated chickens had reduced in vitro mitogenic response; this result indicated that the immunosuppressive regimen caused suppression of t cell function. the immunosuppressed chickens had more circulating hvt and had a s ... | 1980 | 6250430 |
| a transmissible chicken tumour associated with reticuloendotheliosis virus infection. | histiocytic lymphosarcomas of the intestine, liver, spleen and sciatic nerve were found at necropsy in a 36-week-old laying hen that was culled from a flock of 1800 birds because of emaciation. type c particles were observed in ultrathin sections of liver and spleen. the serum of the hen contained reticuloendotheliosis virus (rev) antigen, and antibody against rev, but lacked antibodies reactive with marek's disease virus or subgroups a and b of rous sarcoma virus. the tumour was transmitted to ... | 1980 | 6250524 |
| performance of 3 successive generations of specified-pathogenfree chickens maintained as a closed flock. | no antibodies against salmonella pullorum, mycoplasma gallisepticum, mycoplasma synoviae, haemophilus gallinarum, fowl pox virus, marek's disease virus, herpes virus of turkey, infectious laryngotracheitis virus, avian adenovirus, avian reovirus, infectious bursal disease virus, reticuloendotheliosis virus, avian leukosis virus, avian encephalomyelitis virus and newcastle disease virus were detectable in the sera obtained from these chickens in 3 generations at various ages. antibodies against i ... | 1980 | 6253742 |
| persistence of genomes of both herpesvirus of turkeys and marek's disease virus in a chicken t-lymphoblastoid cell line. | a cell line tentatively designated as mdcc-bo1(t), was established from spleen cells of an apparently healthy chicken inoculated with herpesvirus of turkey (hvt). bo1(t) cells were t lymphoblastoid cells and the more than 95% of them had marek's disease (md) tumor-associated surface antigen (matsa). however, no viral internal antigens or membrane antigens could be demonstrated in them by immunofluorescence tests using chicken anti-hvt and -md virus (mdv) sera. the virus could be rescued from bo1 ... | 1980 | 6254498 |
| [evidence for the persistence of epstein-barr-virus in the parotid gland (author's transl)]. | ebv is associated with b-lymphocytes. it is, however, not clear whether spontaneous activation of ebv genomes in carrier lymphocytes, which are present in the lymphocyte rich area of the oropharynx, is responsible for lifelong persistence of antibody-titers directed against ebv related antigens and the shedding of virus into the oropharynx. alternatively ebv could reside in specific sites of the body and be produced there resembling somehow the situation of marek's disease virus of chicken. that ... | 1981 | 6261052 |
| nature of genetic resistance to marek's disease in chickens. | infection of line 6 resistant and line 7 susceptible chickens with marek's disease virus (mdv) resulted in a depressed phytohemagglutinin (pha) response and the presence of marek's disease (md) tumor-associated surface antigen (matsa) in the spleens. at 6-10 weeks after infection, recovery in pha response, diminution in the number of matsa cells, and the presence of significant anti-matsa immunity were observed in line 6 but not in line 7 chickens. both lines had antibody-dependent cell-mediated ... | 1981 | 6262555 |
| attempts ot activate endogenous virus expression with marek's disease virus dna. | several dnas derived from marek's disease virus-infected cells and tissues were tested for in vitro infectivity and for the ability to activate avian endogenous type c virus. the dna isolated from tumour tissue, peripheral blood buffy coat cells, mdv-infected tissue cultures, lymphoblastoid cell lines and feather follicle epithelium cells from mdv-infected birds elicited a negative response in transfection assays. the mdv dnas isolated did not activate the endogenous type c virus from cell cultu ... | 1981 | 6266886 |
| expression of forssman antigen of avian lymphoblastoid cell lines transformed by marek's disease virus or avian leukosis virus. | the expression of forssman-type heterophile antigen on marek's disease (md) virus (mdv)-transformed cell lines, mdcc-msbi 1, -hp1, -rp1 and -bp1, and avian leukosis virus (alv)-transformed cell lines, lscc-1104b1 and -1104x5 was investigated by membrane immunofluorescence and complement-dependent antibody cytotoxicity tests. forssman antigen was detected on a high percentage of the cells in two alv-transformed cell lines and on a smaller percentage of splenic lymphocytes from normal chicken. of ... | 1981 | 6267170 |
| immune response and disease resistance in chickens. i. selection for high and low titer to salmonella pullorum antigen. | a selection experiment for high and low anti-salmonella pullorum antibody titer was carried out over four generations within the b1b1 blood group genotype in chickens. the study was aimed primarily at identifying different response patterns controlled by immune response genes linked to the b system, the major histocompatibility complex of the chicken. maximal divergence was obtained in the third generation of selection when agglutination titers of 1/320 and 1/80 in b1b1 high and low responders, ... | 1981 | 6267577 |
| serological responses to mycoplasma synoviae in chickens infected with virulent or avirulent strains of marek's disease virus. | the pathogenicity and immunosuppressive capability of five strains of marek's disease herpesvirus (mdhv) was examined. three of the strains (ala, miss, ncs) were isolated from flocks experiencing vaccine breaks while the other two strains (ga, hn) represented prototype virulent and avirulent viruses. pathogenicity of mdhv strains was evaluated on their ability to cause mortality, their ability to cause gross or microscopic lesions, and the median latent period to death (mlpd) for each strain. an ... | 1981 | 6267580 |
| effects of infectious bursal disease on marek's disease vaccination: suppression of antiviral immune response. | studies were made to determine whether infectious bursal disease virus (ibdv) infection would affect the response of chickens to turkey herpesvirus (hvt) vaccination in the development and level of hvt viremia and virus-neutralizing (vn) antibodies to hvt. the hvt viremia in the vaccinated chickens was not affected by ibdv, whether ibdv was inoculated simultaneously with hvt vaccination at one day of age or whether it was inoculated 3 weeks postvaccination with hvt. however, vn antibody response ... | 1980 | 6268042 |
| latency of herpesvirus of turkey and marek's disease virus genomes in a chicken t-lymphoblastoid cell line. | the properties of latent herpesvirus of turkey (hvt) and marek's disease virus (mdv) genomes have been studied in virus-non-producer mdcc-bo1(t) cells, a t-lymphoblastoid cell line derived from spleen cells of an hvt-vaccinated chicken. the numbers of the two virus genomes in bo1(t) cells remained stable at 1.6 to 1.8 hvt genome equivalents/cell and 3.4 to 3.8 mdv genome equivalents/cell throughout a number of passages and were not decreased by the presence of phosphonoacetic acid in the culture ... | 1981 | 6268738 |
| immunoprecipitation of marek's disease virus-specific polypeptides with chicken antibodies purified by affinity chromatography. | 1981 | 6269290 | |
| genetic resistance to herpesvirus-induced lymphoma at the level of the target cell determined by the thymic microenvironment. | 1981 | 6270018 | |
| herpesvirus infection enhances cholesterol and cholesteryl ester accumulation in cultured arterial smooth muscle cells. | in our previous experiments, atherosclerosis similar to that in humans was reproducibly induced in both normocholesterolemic and hypercholesterolemic specific-pathogen-free (spf) chickens by infection with marek's disease herpesvirus (mdv). in contrast, uninfected chickens fed either relatively cholesterol-poor or cholesterol-supplemented diets did not develop this arterial disease. in experiments reported here, the hypothesis that infection of arterial smooth muscle cells (smcs) with mdv would ... | 1981 | 6271018 |
| spontaneous and induced herpesvirus genome expression in marek's disease tumor cell lines. | we incubated 31 newly established marek's disease tumor cell lines at 41 degrees c for 48 h after subculturing and then examined them to determine the spontaneous rates of expression of viral internal antigen(s), viral membrane antigen(s), and virus isolation. all but two of the lines were isolated from tumors induced by clone-purified marek's disease virus strain jm-10, ga-5, rb-1b, and bc-1a in nine different genetic strains of chickens with defined histocompatibility antigens. the line-to-lin ... | 1981 | 6273319 |
| detection of normal and aberrant chromosomes in chicken embryos and in tumor cells. | 1981 | 6275374 | |
| establishment of b-lymphoblastoid cell lines from marek's disease virus-induced tumors in turkeys. | four lymphoblastoid cell lines were established from tumours of turkeys inoculated with high doses of the ga strain of marek's disease virus (mdv). unlike other md lymphoblastoid cell lines of chicken origin, these mdv transformed turkey line appear to be b lymphocytes and produce immunoglobulin. growth characteristics of these cell lines are slightly different; however, they all produce low levels of mdv-specific antigen and carry the complete genome of the virus as determined by virus rescue i ... | 1982 | 6277804 |
| the mechanism of genetic resistance to marek's disease in chickens. | genetic resistance to marek's disease in rpl line-6 chickens is expressed not only at the level of host immunological responses against virus an tumour antigens, but also at the level of target lymphoid cells for virus infection and transformation. the nature of the target cell involved was investigated. spleen cells from susceptible line-7 chickens adsorbed more marek's disease virus and turkey herpesvirus in vitro than line-6 spleen cells. in the case of marek's disease virus this was reflecte ... | 1982 | 6277808 |
| a simple in vitro differentiation between turkey herpesvirus and marek's disease virus. | the growth and plaque formation by turkey herpesvirus (hvt) amd marek's disease herpesvirus (mdhv) were examined in qt35 cells, a continuous fibroblast cell line derived from chemically induced tumors of japanese quail. hvt grew and formed plaques consistently in qt35 cells when inoculated with cell-culture-propagated virus or peripheral mononuclear leukocytes (pml) from chickens that had been inoculated with hvt. both oncogenic and nononcogenic strains of mdhv, however, failed to grow and induc ... | 1981 | 6279072 |
| natural killer cell activity in chickens exposed to marek's disease virus: inhibition of activity in susceptible chickens and enhancement of activity in resistant and vaccinated chickens. | chickens of 2 genetic lines (lines p and n) were inoculated with a pathogenic strain of marek's disease (md) virus (mdv) and chronologically examined for disease response and natural killer (nk) cell expression. the nk cell reactivity was assayed in an in vitro cytotoxicity assay in which effector cells from the spleen of test chickens were reacted with 51cr-labeled lscc-rp9 target cells. chickens of line p developed progressive debilitating disease and a high incidence of gross tumors and death ... | 1981 | 6279075 |
| induction of lymphomas and tumor antigen by marek's disease virus in turkeys. | pathogenic marek's disease (md) virus (mdv) induced a high incidence of mortality and gross and microscopic lesions in turkey poults. inoculated turkey poults became persistently viremic with mdv, although the levels of detectable circulating mdv were generally lower in turkeys than in similarly inoculated chickens. the early lytic phase of md characterized by lymphoid cell destruction and the appearance of viral antigen in lymphoid organ was not as prominent in turkeys as in chickens. gross md ... | 1981 | 6279077 |
| effect of t & b cell immunosuppression on the release of marek's disease virus from the feather follicles of chickens. | 1982 | 6286472 | |
| characterization of an antigen associated with the marek's disease lymphoblastoid cell line msb-1. | a marek's disease lymphoblastoid cell line (msb-1) has been analysed by immunoprecipitation for expression of tumour-associated antigen, marek's disease virus (mdv)-specific antigens and antigens specific to avian leukosis-sarcoma viruses. rabbit antisera raised against two independently derived cell lines after extensive absorption with normal chick cells reacted with a polypeptide of mol. wt. 40 000 (40k) in extracts of msb-1 cells. the 40k polypeptide was not present in myeloblasts or in chic ... | 1982 | 6286857 |
| in vitro infection of lymphocytes with marek's disease virus. | suspension cultures of splenic lymphocytes, incubated at 41 degrees c, became infected with marek's disease virus (mdv) following exposure to a) other infected lymphocytes, b) infected chicken kidney monolayer cultures, or c) cell-free mdv. both viral antigen expression and virus isolation could be demonstrated after more than 40 passages made by the addition of fresh spleen cells at 2- to 3-day intervals. susceptibility of spleen cells from bursectomized chickens was markedly lower than that of ... | 1982 | 6287084 |
| circular forms of viral dna in marek's disease virus-transformed lymphoblastoid cells. | the state of viral dna in three marek's disease virus (mdv)-transformed chicken cell lines (mdcc-msb-1, mdcc-hp-2, mdcc-rp-1) was investigated by cscl-density gradient centrifugation, and sedimentation analysis in neutral glycerol gradients. each cell line contained mdv dna integrated into the host cell genome. additionally, free viral dna could be identified in mdcc-msb-1 and mdcc-hp-2 cells, sedimenting at about 100 s, and banding at the position of circular dna in cscl-ethidiumbromide gradien ... | 1982 | 6287971 |
| inhibitory activity of cytosine arabinoside on marek's disease virus. | the presence of ara-c in growth medium at concentrations of 10(-4) to 10(-7) m completely or partially suppressed the formation of plaques specific for mdv or hvt and decreased proportionally the growth of hprs line 1 lymphoblastoid cells. administration of ara-c to chickens immediately after infection with mdv (1 mg/chicken/day i.p. for 5 days) reduced the incidence of marek's disease by 50%. thus ara-c appears to be an inhibitor of marek's disease. | 1982 | 6293895 |
| a monoclonal antibody reactive with marek's disease tumor-associated surface antigen. | an antibody-secreting hybridoma, rph-6, to marek's disease tumor-associated surface antigen (matsa) was produced by somatic-cell hybridization between the mouse myeloma sp2/o-ag/14 and spleen cells from msb1 immunized mice. the antibody reacted with 95 to 100% of the cells from eight of 10 chicken md cell lines and one of two turkey md cell lines. it did not react with chicken md cell lines rp1 and sk3, turkey md cell line rp19, lymphoid leukosis (ll) cell lines, rp9 and rp12, and reticuloendoth ... | 1983 | 6294177 |
| [relationship between the genetic polymorphism of alkaline phosphatase and the resistance of broiler poultry to marek's disease virus]. | studied was the polymorphism of alkaline phosphatase in blood plasma samples taken from 507 poulets and cocks of lines 66 and 77 of the cornish breed, and lines 88 and 99 of the white plymouth rock breed. the birds were divided into three groups to test their resistance to marek's disease: (1) controls, (2) infected with the virus of marek's disease with 3 subgroups--resistant birds, slightly susceptible ones, and strongly susceptible ones, and (3) contacts, with two subgroups--survivals and bir ... | 1982 | 6294972 |
| influence of maternal antibody on efficacy of embryo vaccination with cell-associated and cell-free marek's disease vaccine. | vaccination with turkey herpesvirus (hvt) of 18-day-old chicken embryos from a commercial source or from a cross (15 x 7) of two inbred lines induced better protection against early post-hatch challenge with virulent marek's disease virus (mdv) than vaccination at hatch, despite the presence in embryos of maternally derived antibodies to hvt or to hvt and mdv. however, 50%-protective-dose (pd50) assays revealed that maternal antibodies in embryos reduced vaccine efficacy. the pd50 assays were co ... | 1982 | 6297450 |
| marek's disease virus (mdv) antigens in the feather follicle epithelium: difference between oncogenic and nononcogenic mdv. | feather tips from chickens infected with oncogenic or nononcogenic marek's disease virus (mdv) were individually tested for mdv antigens by the agar-gel-precipitin test to compare antigen contents of feather follicles infected with oncogenic or nononcogenic mdv. there was a significant difference between oncogenic and nononcogenic mdv in the development of mdv antigens in the feather follicle epithelium of infected chickens. significantly more feather tips from chickens infected with oncogenic m ... | 1982 | 6297453 |
| suppression of humoral immunity in chickens prevents transient paralysis caused by a herpesvirus. | marek's disease virus (mdv)3 is a highly oncogenic herpesvirus that usually causes visceral lymphomas and lymphoid infiltration of the peripheral nerves in chickens. a relatively rare encephalitic condition, first found in farm flocks and referred to as transient paralysis (tp), is also caused by mdv(1). tp symptoms occur 9 to 11 days after mdv inoculation and range from mild ataxia to profound coma. most birds recover by 24 to 72 hr after onset of symptoms, although severely affected birds may ... | 1983 | 6300237 |
| characterization of marek's disease virus-infected lymphocytes: discrimination between cytolytically and latently infected cells. | leukocyte suspensions derived from genetically marek's disease (md)-resistant n-line and md-susceptible p-line chickens were fractionated at various times after exposure to the jm-10 clone of md virus. at 3 and 5 days post exposure (dpe), during the productive-restrictive (cytolytic) phase, most infected spleen and thymus leukocytes were found to be low-density, nylon wool-adherent cells that possessed fc receptors and surface ia and igm and were depleted by carbonyl iron treatment. this was tru ... | 1983 | 6300499 |
| failure of herpesvirus saimiri to enhance atherogenesis in owl monkeys (aotus trivirgatus). | a retrospective study was performed to evaluate the potential of a mammalian oncogenic virus, herpesvirus saimiri, to cause atherosclerosis in owl monkeys (aotus trivirgatus). this was undertaken since an avian oncogenic herpesvirus, marek's disease virus, does so in chickens. data from earlier studies were reviewed and 3 groups of animals were selected. these included 23 animals infected with herpesvirus that died an average of 156 days later with malignant lymphoma; 11 infected an average of 2 ... | 1983 | 6301514 |
| embryo vaccination against marek's disease with serotypes 1, 2 and 3 vaccines administered singly or in combination. | marek's disease virus (mdv) vaccines of serotypes 1 and 2 administered in 18-day-old embryonated eggs induced better protection against post-hatch challenge at 3 days with virulent mdv than vaccines given at hatch. embryonal vaccination with a polyvalent vaccine containing equal quantities of serotypes 1 and 2 of mdv and serotype 3 virus (turkey herpesvirus, hvt) was also significantly more effective than post-hatch vaccination. these and earlier results indicate that protective efficacy of sing ... | 1983 | 6307248 |
| studies on the role of macrophages in marek's disease of the chicken. | macrophage activity in relation to marek's disease was investigated by determining phagocytic indices in vivo and by examining virus plaque-inhibiting activity of peritoneal macrophages in vitro. no correlation was observed between phagocytic index and resistance in different genetic strains of chickens. infection with marek's disease virus increased both phagocytic indices and the plaque-inhibiting activity of peritoneal macrophages, more so in susceptible than in resistant chickens. there was ... | 1983 | 6312038 |
| simultaneous vaccination of one-day-old broiler chickens with tenosynovitis virus and turkey herpesvirus. | an attenuated tenosynovitis virus vaccine and turkey herpesvirus (hvt) vaccine mixed prior to vaccination were injected subcutaneously into one-day-old chicks. in laboratory and field trials there did not appear to be any interference, because chickens vaccinated with both vaccines were effectively immunized against challenge with virulent marek's disease virus or tenosynovitis virus when compared with unvaccinated chickens or chickens vaccinated with either hvt or tenosynovitis virus. the combi ... | 1983 | 6312445 |
| viruses in the etiology of atherosclerosis. | to examine the possible role of viruses in the etiology of atherosclerosis, we searched for the presence of viral genomes in arterial tissues by in situ hybridization. because chickens infected with marek disease virus, a herpesvirus, develop atherosclerotic lesions after infection, we looked for the presence of herpesvirus or parts thereof in human artery wall tissue, particularly in individuals with evidence of atherosclerosis. herpesvirus probes were used on specimens of aortic wall removed f ... | 1983 | 6312457 |
| establishment of lymphoblastoid cell lines from marek's disease primary tumors. | seven lymphoblastoid cell lines were developed from 15 marek's disease primary lymphomas treated to remove adherent cells (46.7% success rate) and 1 cell line was developed from 15 untreated lymphomas (6.7% success rate). these cell lines are designated as mdcc-rp22 through mdcc-rp29. tumor materials were from 151(5) x 7(1) chickens infected with either jm-111s clone or the ga-22 clone of marek's disease virus. factors contributing to the establishment of cell lines include, in addition to the r ... | 1983 | 6314315 |
| kinetics of phytohemagglutinin response in chickens infected with various strains of marek's disease virus. | phytohemagglutinin (pha) response of whole blood lymphocytes from white leghorn inbred line 7(2) chickens infected with various strains of marek's disease virus (mdv) was monitored sequentially for 6 weeks postinfection. a significant difference between jm and ga strains was shown. a two-phase depression in the pha response was observed in chickens infected with the jm strain. early depression occurred 1 week postinfection and was followed by recovery a week later. the second depression occurred ... | 1983 | 6314973 |
| marek's disease virus-induced transient paralysis: a comparison of lesions in susceptible and resistant lines of chickens. | a study was conducted to determine the causative lesion of marek's disease virus-induced transient paralysis. brain histologic lesions were quantitated and compared in genetically resistant and susceptible inbred chickens. comparisons were made 11 days post inoculation (pi) of virus when clinical signs were evident in susceptible birds and on day 17 pi when these signs had remitted. clinically-affected birds from the susceptible line on day 11 pi had consistent evidence of neuropil vacuolation c ... | 1983 | 6316708 |
| in vivo characteristics of a transplantable marek's disease lymphoblastoid cell line, mdcc-msb1-41c. | a marek's disease (md) lymphoblastoid cell line, mdcc-msb1-41c, was highly transplantable and lethal for chickens. autopsies showed extensive metastasis in various organs. the transplantabilities of the parent cell line, mdcc-msb1, and another derivative line, mdcc-msb1-33c, were transient. md virus (mdv) could be isolated from the kidneys but not from the peripheral blood leukocytes of chickens inoculated with the msb1-41c cell line. in addition, anti-mdv antibodies were produced both in chicke ... | 1983 | 6316900 |
| [morphological studies of lymphatic tissue of chick livers following infection with marek's disease virus and turkey herpes virus]. | 1983 | 6318683 | |
| further characterization of marek's disease virus-infected lymphocytes. i. in vivo infection. | previous reports from this laboratory identified bursa-derived lymphocytes (b cells) and non-b cells as the predominant cell types respectively involved in the early cytolytic and subsequent latent infection of chickens with marek's disease virus (mdv). it was not known whether these differences were qualitative or quantitative or if the method for detection of latent infection (viral antigen production after 48 h of in vitro cultivation) was sensitive enough. to further define the cells involve ... | 1984 | 6321364 |
| further characterization of marek's disease virus-infected lymphocytes. ii. in vitro infection. | lymphocyte cultures from chicken spleens had been shown to be susceptible to in vitro infection by marek's disease virus (mdv)4 in an earlier report from this laboratory. in that study, virus infection was evidenced by virus isolation and detection of viral internal antigen (via) 2 days post inoculation (dpi), and serial passage was accomplished by adding fresh spleen cells at 2-day intervals. the susceptible cells were identified as bursa-derived lymphocytes (b cells). using a dual fluorescence ... | 1984 | 6321365 |
| identification with monoclonal antibodies of glycoproteins of marek's disease virus and herpesvirus of turkeys related to virus neutralization. | by use of monoclonal antibodies cross-reactive with marek's disease virus and herpesvirus of turkeys, three glycoproteins (for marek's disease virus, gp115/110, gp63, and gp50; for herpesvirus of turkeys, gp115, gp62 and gp52) related to virus neutralization were identified. immunization of chickens or rabbits with these glycoproteins purified by affinity chromatography resulted in production of neutralizing antibodies. | 1984 | 6321752 |
| [vaccinating properties of the kekava 55 strain of attenuated marek's disease virus]. | two attenuated strains of oncogenic herpes virus, marek's disease virus, were obtained: one by cultivation in chick fibroblast cultures at 37 degrees c, the other at 41 degrees c, i. e. at chicken body temperature. both attenuated strains throughout a large number of passages retain high vaccination efficacy, on the average 76% and 77.3%, respectively. the virus attenuated at 41 degrees c is superior to that attenuated at 37 degrees c in higher reproduction rate in vitro and, possibly, in a grea ... | 1983 | 6322454 |
| [early mortality syndrome in chickens following double infection with marek's disease virus and chicken anemia agent]. | 1983 | 6322482 | |
| in vivo and in vitro infections of chicken lymphocytes with marek's disease virus or herpesvirus of turkeys. | 1983 | 6323800 | |
| extensive homology exists between marek disease herpesvirus and its vaccine virus, herpesvirus of turkeys. | marek disease is a lymphomatous disease of chickens caused by infection of a herpesvirus, marek disease virus (mdv). marek disease is the only neoplastic disease for which a successful vaccine has been developed. the vaccine virus, herpesvirus of turkeys (hvt), is non-oncogenic in chickens. despite the strong antigenic relationship between these viruses, previous studies showed that the two viral dnas share little or no homology. using less stringent hybridization conditions and methods that gre ... | 1984 | 6328512 |
| cell-mediated immunity in marek's disease: cytotoxic responses in resistant and susceptible chickens and relation to disease. | two experiments were conducted to study the cell-mediated cytotoxicity of peripheral blood leukocytes (pbl) from chickens inoculated with marek's disease virus (mdv) against a marek's disease-derived lymphoblastoid cell line (msb-1) and to associate the cytotoxicity with incidence of disease. in experiment i, moderately susceptible random-bred, specific-pathogen-free chickens were inoculated with mdv (group 1), vaccinated with a herpesvirus of turkeys (hvt) and inoculated with mdv (group 2), vac ... | 1980 | 6445171 |
| in vitro suppression of t-cell mitogenic response and tumor cell proliferation by spleen macrophages from normal chickens. | adherent cells isolated from spleen of normal specific pathogen-free chickens inhibited mitogen-induced blastogenesis of autochthonous, syngeneic, or allogeneic lymphocytes. the adherent cells were also inhibitory to in vitro proliferation of cells of a rapidly dividing tumor line, mdcc-msb-1, derived from a lymphoma induced by marek's disease virus. the effector cell of suppression of both lymphoprolifrative functions appeared to be a macrophage because the suppressive activity of adherent cell ... | 1980 | 6447112 |
| [genetic polymorphism of alkaline phosphatase and the resistance to marek's disease virus]. | experiments were carried out with a total of 222 birds of the 5-a, 6-e, ya-73, ll, and ls lines of the white leghorn breed to find whether or not there existed any correlation between the alkaline phosphatase allels and the resistance to marek's disease. the polymorphism of alkaline phosphatase was evaluated in the blood plasma of birds through horizontal electrophoresis in starch gel after g a hn e [3]. the resistance of the five egg laying lines of birds was found to be associated with the akp ... | 1983 | 6581645 |
| presence of adherent cytotoxic cells and non-adherent natural killer cells in progressive and regressive marek's disease tumors. | marek's disease virus-induced progressive tumors and marek's disease transplantable local regressive tumors were dispersed by treatment with collagenase and cells were examined in vitro for cytotoxic effector functions against target cells of tumor lines. both types of tumors had adherent and non-adherent cytotoxic cells. the cytotoxicity of adherent cells was detected in an 18-hour but not in a 4-hour 51cr-release assay. the adherent effector cells from progressive tumors were inactivated by pr ... | 1983 | 6606892 |
| prediction of the occurrence of marek's disease in chickens on the basis of the quantitation of cell-mediated immunity. | cell mediated immunity was assayed in chickens on the basis of the dinitrochlorobenzene skin test, t lymphocyte numbers in peripheral blood and the graft-versus-host reaction. it was found that chickens that were to develop clinical marek's disease following exposure to marek's disease virus had significantly lower prechallenge levels of cell mediated immunity than those that were resistant to marek's disease. the graft-versus-host reaction had a smaller coefficient of variation than the other t ... | 1984 | 6609388 |
| marek's disease virus-induced transient paralysis: clinical and electrophysiologic findings in susceptible and resistant lines of chickens. | results of neurologic examination, eeg, and motor nerve conduction velocity quantitation were analyzed in a line of chickens susceptible to marek's disease virus-induced transient paralysis and compared with findings in a resistant line. both lines were evaluated on the day before virus inoculation (day 1; base line) and on days 12 and 19. the susceptible birds frequently became depressed and paretic on day 11 or 12 and then recovered, and the resistant line rarely was affected clinically. the e ... | 1983 | 6625305 |
| two sublines of a lymphoblastoid cell line associated with marek's disease virus. | from mdcc-msb1, the original marek's disease virus-associated lymphoblastoid cell line, two sublines named mdcc-msb1-33c and mdcc-msb1-41c were differentiated. the mdcc-msb1-33c cell line grew well at 33 degrees, but grew poorly and finally stopped growing at 41 degrees. on the other hand, the mdcc-msb1-41c cell line grew actively at 41 degrees, but at 33 degrees, continuous cultivation was difficult to achieve. mdcc-msb1-33c grew suspended in the liquid medium, as did the original producer mdcc ... | 1983 | 6628902 |
| [resistance of different breeds of laying poultry to marek's disease virus]. | investigated were a total of 447 two-day-old chicks of lines 5-a, ia-73' ll and ls of the leghorn breed divided into 2 groups: controls--99 males and females, and test ones--348 infected at the age of 2 days via intraabdominal injection with 0.4 cm3 heparinized blood each, containing 5000 pfu per dose of the marek's disease virus (ct-1 isolate). both linear and sexual variations were found in the resistance of the birds. most resistant proved to be the 5-a chicks of both sexes, and most suscepti ... | 1983 | 6659344 |
| the occurrence of chicken foetal antigen after infection with marek's disease virus in three strains of chicken. | an indirect immunofluorescence procedure employing specific antiserum was used to demonstrate chicken foetal antigen (cfa), a membrane antigen typically present on embryonic cells. the expression of cfa by lymphocytes from the peripheral blood, spleen, thymus and bursa was found to decline rapidly after hatching; the bursa, however, contained about 30% cfa-positive cells until at least 120 days of age. after infection of a genetically susceptible strain of chickens (hprs rhode island red) with m ... | 1983 | 6835849 |
| herpesvirus-induced atherosclerosis in chickens. | repeated experiments have established that infection with marek's disease herpesvirus (mdv) leads to atherosclerosis in specific pathogen free (spf) normocholesterolemic chickens. neither normocholesterolemic nor hypercholesterolemic uninfected spf chickens develop this disease. the mdv-induced arterial disease is remarkably similar to chronic human atherosclerosis. cholesterol and saturated cholesteryl esters accumulated in cultured arterial smooth muscle cells (smc) infected with mdv. similar ... | 1983 | 6840298 |
| natural killer cell activity in chickens: target cell analysis and effect of antithymocyte serum on effector cells. | a battery of lymphoid and nonlymphoid cells was examined for susceptibility to lysis by natural killer cells of chickens. several susceptible targets were recognized, and most susceptible among these were cells of line lscc-rp9, derived from a lymphoid tumor induced by rous-associated virus 2. the natural killer reactivity against lscc-rp9 target cells did not appear to be directed against an antigen(s) induced by rous-associated virus 2 because other lymphoid and nonlymphoid cells infected with ... | 1981 | 6971810 |
| independence of chicken major histocompatibility antigens and tumor-associated antigen on the surface of herpesvirus-induced lymphoma cells. | capping of chicken major histocompatibility (mhc) antigens on normal thymus, spleen, and peripheral blood leukocytes was demonstrated, although mhc antigens appeared to be present on only 15 to 18% of normal thymus cells. mhc antigen capping also occurred on cells from a marek's disease herpesvirus-induced transplantable lymphoma (mdct-nym1). capping of a marek's disease tumor-associated surface antigen (matsa) could be induced on mdct-nym1 lymphoma cells as well as on cells of two marek's disea ... | 1980 | 7000703 |
| transplantable marek's disease lymphomas. i. growth characteristics during development in two inbred lines of chickens. | lymphomas developed in the pectoral muscle of most chickens inoculated with cells from primary marek's disease virus-induced visceral tumors obtained from chickens of the same inbred line. however, serial passaging of the lymphoma cells in histocompatible hosts generally resulted either in an eventual absence of tumor formation at the inoculation site or in tumor regression. exceptions occurred in two experiments, where tumors grew rapidly and the hosts died early. subsequent passaging of cells ... | 1982 | 7103886 |
| chronological observations of feather pulp lesions in chickens inoculated with marek's disease virus. | observations of the feather pulp from chickens inoculated with marek's disease (md) virus revealed diverse lesions similar to those in the peripheral nerves of chickens infected with md. these lesions were categorized as follows: r1-type lesions = from minimal perivascular to diffuse infiltration of small lymphocytes mixed with a few medium lymphocytes or blast cells; r2-type lesion = edema and cellular infiltration consisting of plasma cells and small lymphocytes; and t-type lesion = tumorous p ... | 1982 | 7103893 |
| marek's disease as a model for the landry--guillain--barré syndrome: latent viral infection in nonneuronal cells accompanied by specific immune responses to peripheral nerve and myelin. | in the chicken, marek's disease virus (mdv) induces a demyelinating peripheral neuropathy that, early in the course of the disease, is histopathologically indistinguishable from that seen in the landry--guillain--barré syndrome in man. a continuing role for a productive infection in the pathogenesis of this disease is unlikely, since neither mdv nor mdv antigens can be characteristically detected in nerves or spinal ganglia examined at necropsy. the authors investigated the possible role of a la ... | 1981 | 7234967 |
| the in vivo effect of levamisole on phytohaemagglutinin stimulation of lymphocytes in normal and marek's disease virus inoculated chickens. | 1981 | 7255915 | |
| reduced vaccinal protection of turkey herpesvirus against field strains of marek's disease herpesvirus. | four isolates of marek's disease herpesvirus (mdv) were obtained from 21-week-old broiler breeders with lesions and mortality from marek's disease (md). they had been properly vaccinated with turkey herpesvirus (hvt). the isolates were designated ala-7, 8, 9, and 10. these 4 mdv strains were compared with the ga isolate of mdv in chickens vaccinated with graded doses of hvt. challenge with the mdv strains was also with graded doses. all 5 isolates of mdv were similar in virulence. however, prote ... | 1981 | 7267529 |
| cell-mediated immunity in marek's disease virus-infected chickens genetically selected for high and low concentrations of plasma corticosterone. | lines of chickens genetically selected for high (hpc)-and low-plasma corticosterone (lpc) titers were assessed for cell-mediated immunity during marek's disease tumorigenesis. lymphocyte transformation (using phytohemagglutinin and 2,4-dinitrofluorobenzene-bovine serum complex) and mitogen-induced cellular cytotoxicity were evaluated with peripheral blood leukocytes. chickens from the lpc line showed a greater lymphocyte transformation response than those from the hpc line. all tumor-bearing chi ... | 1980 | 7362128 |
| endothelial mhc class ii antigen expression and endarteritis associated with marek's disease virus infection in chickens. | experimental marek's disease virus (mdv) infection in chickens was used to study the early pathogenesis of virus-induced atherosclerosis. previous investigations using this model have reported the occurrence of atherosclerotic lesions after approximately 7 months postinfection. in this study, a total of 75 susceptible cornell p-line chickens were inoculated intraperitoneally with the cu-2 strain of mdv at 3 days of age and subsequently perfused for histologic examination. at 2, 4, 8, 13, and 20 ... | 1995 | 7483215 |
| virus-neutralization domains on the oligomeric (230 kda) forms of antigen b of herpesvirus of turkeys and marek's disease virus differ in cross-serotypic activity. | herpesvirus of turkeys (hvt) is frequently used to protect chickens against marek's disease (md). the hvt and mdv native antigen b complex shares common epitopes. to determine whether these oligomers present virus-neutralizing domains, monospecific antibodies to the hvt and mdv native 230 kda oligomers were produced. the monospecific antibody immunopurified from an anti-hvt avian serum neutralized the in vitro infectivity of the oncogenic isolate mdv-b and the vaccine strains cvi988, sb1 and hvt ... | 1995 | 7483903 |
| retention of oncogenicity by a marek's disease virus mutant lacking six unique short region genes. | we previously reported the construction of marek's disease virus (mdv) strains having mutations in various genes that map to the unique short (us) region of the viral genome (j.l. cantello, a.s. anderson, a. francesconi, and r.w. morgan, j. virol. 65:1584-1588, 1991; m.s. parcells, a.s. anderson, and r.w. morgan, virus genes 9:5-13, 1994; m.s. parcells, a.s. anderson, and r.w. morgan, j. virol. 68:8239-8253, 1994). these strains were constructed by using a high-passage-level serotype 1 mdv strai ... | 1995 | 7494301 |
| common antigenic epitopes are present on heat-labile oligomers of mdv glycoprotein b and on hsv glycoprotein b. | the antigenic cross-reactivity between the marek's disease virus glycoprotein b (mdv gb) and glycoprotein b (gb) of herpes simplex virus type 1 and 2 (hsv1 and hsv2) was analysed by the immunoblotting method. we studied cell lysates in both denatured and in undenatured form (i.e., unheated) and reacted them with convalescent sera from chickens infected with the rbib mdv strain and with human anti-hsv1 gb. both sera detected the heat-labile mdv gb and the hsv gb oligomers. in addition, monospecif ... | 1995 | 7540344 |
| influence of the alv6 recombinant avian leukosis virus transgene on production traits and infection with avian tumor viruses in chickens. | the biological costs of the alv6 recombinant transgene that in chickens induces dominant resistance to the subgroup a avian leukosis virus (alv), in terms of effects on production traits, were studied. four generations of white leghorn chickens of line tr, segregating for alv6 but free of endogenous viral genes, as well as two generations of crosses between tr and ottawa line wg (wgtr) were tested under a specific-pathogen-free environment. in the birds studied, the transgene appeared unchanged ... | 1995 | 7603962 |
| sequence characteristics of a gene in infectious laryngotracheitis virus homologous to glycoprotein d of herpes simplex virus. | an infectious laryngotracheitis virus (iltv, gallid herpesvirus 1) gene homologous to glycoprotein d of herpes simplex virus (hsv) was identified and characterized by its nucleotide and derived amino acid sequence. the iltv gd gene is located in the unique short region (u(s)) and contains an open reading frame capable of specifying a polypeptide of 380 amino acids, including n- and c- terminal hydrophobic domains consistent with signal and anchor regions respectively, and no potential sites for ... | 1995 | 7612933 |
| herpesvirus of turkey recombinant viruses expressing infectious bursal disease virus (ibdv) vp2 immunogen induce protection against an ibdv virulent challenge in chickens. | two recombinant herpesviruses of turkey (hvt) expressing the vp2 protein of infectious bursal disease virus (ibdv or gumboro disease virus) have been constructed: vhvt001 and vhvt002. the vp2 open reading frame was inserted at the locus of the small subunit of ribonucleotide reductase gene (hsv-1 ul40 homolog) without any exogenous promoter in vhvt001 and at the locus of gl gene (hsv-1 us7 homolog) under the control of the human cytomegalovirus immediate-early promoter in vhvt002. the isolation ... | 1995 | 7645252 |
| measurement of antibody titer to fowl pox virus by enzyme-linked immunosorbent assay. | the usefulness of the measurement of antibody titer to fowl pox virus (fpv) by enzyme-linked immunosorbent assay (elisa) was evaluated in spf chickens with or without inoculation with fpv. the optimum concentration of purified antigen was 10 micrograms/ml of protein. the absorbance at 492 nm was less than 0.10 in the chickens negative to fpv from 1 to 63 days old. by contrast, a higher titer was detected in spf chickens with various fpvs inoculated into the wing web than in non-inoculated chicke ... | 1994 | 7696418 |
| cytogenetic studies of cell lines derived from marek's disease virus-induced local lesions. | cytogenetic analysis of cell lines derived from marek's disease virus (mdv) induced local lesions was performed to detect the presence of a chromosomal alteration found previously in cell lines derived from mdv-induced visceral tumors. this chromosomal alteration involves an amplified region on the short arm of chromosome 1. trypsin g-binding was performed on 12 local-lesion cell lines having various t-cell receptor phenotypes. eight of 12 cell lines had a diploid female karyotype, and four line ... | 1994 | 7702513 |
| partial inhibition by turkey herpesvirus of serotype 2 marek's disease virus plaque formation and in vivo infectivity. | the increased use of serotype 2 marek's disease virus (mdv) and serotype 3 turkey herpesvirus (hvt) as components of effective bivalent vaccines against marek's disease (md) prompted studies on the possible interactions of these two viruses in vitro and in vivo. the replication of the sb-1 strain of mdv was compared with replication of the fc126/2 strain of hvt in chickens and cell cultures infected with one or both viruses. replication of mdv was reduced in the presence of hvt in both in vitro ... | 1994 | 7702514 |
| use of recombinant pp38 antigen of marek's disease virus to identify serotype 1-specific antibodies in chicken sera by western blotting. | a fowlpox recombinant expressing the pp38 antigen of marek's disease virus has been constructed. production of pp38 in chick embryo fibroblasts (cef) infected at a m.o.i. of 1 pfu/cell occurred over a period of 5 days and reached a peak at 72 h after infection. the pp38 antigen could be released from infected cells by freezing and thawing. western blot analysis showed that denatured pp38 antigen reacted with antisera from chickens inoculated with serotype 1 mdv but failed to react with antisera ... | 1994 | 7714041 |