Publications

serological evidence for influenza virus infection in korean wild boars.	serum samples from 1,011 wild boars hunted in 2012 were collected for serological surveillance for 4 subtypes (pandemic a (h1n1) 2009 and classical h1n1, h1n2 and h3n2) of swine influenza virus (siv). samples from 12 of the boars were identified as positive for siv (pandemic a (h1n1) 2009, n=9; classical h1n1, n=2; and h1n2, n=1) by a hemagglutination inhibition test (hi test) and a nucleoprotein (np)-based enzyme-linked immunosorbent assay (np-elisa). although the overall seroprevalence of siv ...	2014	25298239
efficacy and safety of novel epicardial circumferential left atrial ablation with pulmonary vein isolation in sustained atrial fibrillation.	the aim of this study was to examine the efficacy and safety of this novel epicardial circumferential left atrial ablation (claa) with pulmonary vein isolation (pvi) in sustained atrial fibrillation (af). thirty domestic pigs were divided equally into 3 groups: af without ablation (af group), af with pvi (pvi group), and af with claa and pvi (claa + pvi group). af was induced by rapid atrial pacing. after af was induced, claa and pvi were performed for pigs in claa + pvi group, and pvi was perfo ...	2015	25323730
molecular epizootiology and diagnosis of porcine babesiosis in sardinia, italy.	the recent characterization of the 18s ribosomal rna (rrna) of a pathogenic babesia species in a domestic sow paved the way for establishing diagnostic and epidemiological tools for porcine babesiosis. here, we developed the first specific babesia sp. suis pcr, and we applied this test to a panel of samples collected from animals living in a typical mediterranean environment (sardinia, italy), including domestic pigs, wild boars, and ticks. in domestic pigs, pcr coupled with sequencing revealed ...	2014	25325315
a genome-wide scan for selection signatures in yorkshire and landrace pigs based on sequencing data.	pigs have experienced dramatic selection due to domestication, which has led to many different phenotypes when compared to their wild counterparts, especially in the last several decades. currently, genome-wide scans in both cattle and humans showing positive selection footprints have been investigated. however, few studies have focused on porcine selection footprints, particularly on a genome-wide scale. surveying for selection footprints across porcine genomes can be quite valuable for reveali ...	2014	25327778
pathogenic enterobacteria in lemurs associated with anthropogenic disturbance.	as human population density continues to increase exponentially, speeding the reduction and fragmentation of primate habitat, greater human-primate contact is inevitable, making higher rates of pathogen transmission likely. anthropogenic effects are particularly evident in madagascar, where a diversity of endemic lemur species are threatened by rapid habitat loss. despite these risks, knowledge of how anthropogenic activities affect lemur exposure to pathogens is limited. to improve our understa ...	2014	25328106
anticipation and frequency of feeding affect heart reactions in domestic pigs.	measuring heart reactions has become a widely used method for the assessment of emotions. heart rate and its variability, which can quite easily be noninvasively recorded, reflect the inputs of the sympathetic and parasympathetic branches of the autonomous nervous system. we tested the hypothesis that frequent anticipation of a positive event results in an increased state of welfare in pigs, expressed as positive arousal in anticipation of announced feeding as well as lowered heart rate and augm ...	2014	25349338
immune modulation in xenotransplantation.	the use of animals as donors of tissues and organs for xenotransplantations may help in meeting the increasing demand for organs for human transplantations. clinical studies indicate that the domestic pig best satisfies the criteria of organ suitability for xenotransplantation. however, the considerable phylogenetic distance between humans and the pig causes tremendous immunological problems after transplantation, thus genetic modifications need to be introduced to the porcine genome, with the a ...	2014	25354539
advances in research in animal models of burn-related hypertrophic scarring.	skin burn injuries affect approximately 500,000 people per year in france. after deep burns, functional sequelae associated with hypertrophic and retractile scars are an important public health problem. to understand the pathophysiology of sequelae and evaluate new therapeutic approaches, the use of animal models that should be standard tools is necessary. some pre-clinical models of hypertrophic scars after burns have been described, but the choice of the appropriate and relevant experimental m ...	2015	25356852
evaluation of genome based estimated breeding values for meat quality in a berkshire population using high density single nucleotide polymorphism chips.	the accuracy of genomic estimated breeding values (gebv) was evaluated for sixteen meat quality traits in a berkshire population (n = 1,191) that was collected from dasan breeding farm, namwon, korea. the animals were genotyped with the illumina porcine 62 k single nucleotide polymorphism (snp) bead chips, in which a set of 36,605 snps were available after quality control tests. two methods were applied to evaluate gebv accuracies, i.e. genome based linear unbiased prediction method (gblup) and ...	0	25358312
in the united states, negligible rates of zoonotic sarcocystosis occur in feral swine that, by contrast, frequently harbour infections with sarcocystis miescheriana, a related parasite contracted from canids.	transmission of pathogens between domestic and wild life animals plays an important role in epidemiology. feral pig populations are increasing and expanding in the usa, and may constitute a risk to non-biosecure domestic pig facilities by serving as reservoirs for pathogens. we surveyed, for sarcocystis infection, the myocardium of 1006 feral pigs (sus scrofa) trapped or hunted in 29 states during the comprehensive feral swine disease surveillance program of the usda's animal and plant health in ...	2015	25363485
emergence of 2.1. subgenotype of classical swine fever virus in pig population of india in 2011.	limited studies are available on molecular epidemiology of classical swine fever virus (csfv) in india and are restricted to domestic pigs. these studies show the presence of 1.1. genotype.	2014	25365116
safety and biocompatibility of a novel self-expanding nitinol carotid stent with hybrid cell design in a porcine model of neointimal hyperplasia.	stent design may influence the outcomes, suggesting that adverse event rates vary according to free cell area and cell design. open cell design technology of self-expandable stents, dedicated for carotid revascularisation has better deliverability, although closed cell technology is expected to cause fewer thromboembolic events.	2015	25371312
ovine β-lactoglobulin at atomic resolution.	the crystal structure of the triclinic form of the milk protein β-lactoglobulin from sheep (ovis aries) at 1.1 å resolution is described together with a comparison of the triclinic structures of the low-ph bovine and high-ph ovine proteins. all three structures are remarkably similar, despite the well known ph-dependent conformational transition described for the bovine and porcine proteins that occurs in solution. the high resolution of the present structure determination has allowed a more acc ...	2014	25372816
effects of intra-aortic balloon pump counterpulsation on left ventricular mechanoenergetics in a porcine model of acute ischemic heart failure.	we investigated the effects of intra-aortic balloon pump (iabp) counterpulsation on left ventricular (lv) contractility, relaxation, and energy consumption and probed the underlying physiologic mechanisms in 12 farm pigs, using an ischemia-reperfusion model of acute heart failure. during both ischemia and reperfusion, iabp support unloaded the lv, decreased lv energy consumption (pressure-volume area, stroke work), and concurrently improved lv mechanical performance (ejection fraction, stroke vo ...	2014	25376149
endothelin-a receptor antagonism after renal angioplasty enhances renal recovery in renovascular disease.	percutaneous transluminal renal angioplasty/stenting (ptras) is frequently used to treat renal artery stenosis and renovascular disease (rvd); however, renal function is restored in less than one half of the cases. this study was designed to test a novel intervention that could refine ptras and enhance renal recovery in rvd. renal function was quantified in pigs after 6 weeks of chronic rvd (induced by unilateral renal artery stenosis), established renal damage, and hypertension. pigs with rvd t ...	2014	25377076
occurrence, source, and human infection potential of cryptosporidium and enterocytozoon bieneusi in drinking source water in shanghai, china, during a pig carcass disposal incident.	in march 2013, thousands of domestic pig carcasses were found floating in the huangpu river, a drinking source water in shanghai, china. to investigate the impact of the pig carcass incident on microbial water quality, 178 river water samples were collected from the upper huangpu river from march 2013 to march 2014. samples were concentrated by calcium carbonate flocculation and examined for host-adapted cryptosporidium and enterocytozoon bieneusi by ploymerase chain reaction (pcr). positive pcr ...	2014	25383482
volatile emission of decomposing pig carcasses (sus scrofa domesticus l.) as an indicator for the postmortem interval.	this study aimed at correlating selected carcass borne volatile organic compounds (vocs) with the postmortem interval (pmi). selected volatiles should 1st be reliably emitted during vertebrate decay, 2nd be emitted at high concentrations, and 3rd show a reproducible quantitative dynamic during the decaying process. four pigs (sus scrofa domesticus l.) were placed in a deciduous forest in different seasons and volatiles emitted during the decaying process were sampled. seventeen compounds were id ...	2015	25387588
laparoscopic anterior resection: new anastomosis technique in a pig model.	bowel anastomosis after anterior resection is one of the most difficult tasks to perform during laparoscopic colorectal surgery. this study aims to evaluate a new feasible and safe intracorporeal anastomosis technique after laparoscopic left-sided colon or rectum resection in a pig model.	0	25392669
reproductive physiology and ovarian folliculogenesis examined via 1h-nmr metabolomics signatures: a comparative study of large and small follicles in three mammalian species (bos taurus, sus scrofa domesticus and equus ferus caballus).	the aim of this study was to characterize the composition of follicular fluid (ff) collected from the small and large follicles of three mammalian species, bos taurus, sus scrofa domesticus, and equus ferus caballus, that display distinct ovulatory properties. for each species, five large ff samples and five small ff samples were analyzed using 1h-nmr spectroscopy. the ff metabolic profiles of the three species were very distinct. in cows and mares, the metabolic profiles of large ff and small f ...	2015	25393852
bupivacaine mandibular nerve block affects intraoperative blood pressure and heart rate in a yucatan miniature swine mandibular condylectomy model: a pilot study.	the primary objective was to evaluate the effect of a bupivacaine mandibular nerve block on intraoperative blood pressure (bp) and heart rate (hr) in response to surgical stimulation and the need for systemic analgesics postoperatively. we hypothesized that a mandibular nerve block would decrease the need for systemic analgesics both intraoperatively and postoperatively.	2014	25394295
direct detection and differentiation of pathogenic leptospira species using a multi-gene targeted real time pcr approach.	leptospirosis is a growing public and veterinary health concern caused by pathogenic species of leptospira. rapid and reliable laboratory tests for the direct detection of leptospiral infections in animals are in high demand not only to improve diagnosis but also for understanding the epidemiology of the disease. in this work we describe a novel and simple taqman-based multi-gene targeted real-time pcr approach able to detect and differentiate leptospira interrogans, l. kirschneri, l. borgpetere ...	2014	25398140
comparison of the genomic sequence of the microminipig, a novel breed of swine, with the genomic database for conventional pig.	the microminipig, which weighs less than 10 kg at an early stage of maturity, has been reported as a potential experimental model animal. its extremely small size and other distinct characteristics suggest the possibility of a number of differences between the genome of the microminipig and that of conventional pigs. in this study, we analyzed the genomes of two healthy microminipigs using a next-generation sequencer solid™ system. we then compared the obtained genomic sequences with a genomic d ...	2014	25398807
individualized early goal-directed therapy in systemic inflammation: is full utilization of preload reserve the optimal strategy?	in severe acute pancreatitis, the administration of fluids in the presence of positive fluid responsiveness is associated with better outcome when compared to guiding therapy on central venous pressure. we compared the effects of such consequent maximization of stroke volume index with a regime using individual values of stroke volume index assessed prior to severe acute pancreatitis induction as therapeutic hemodynamic goals.	2014	25402295
genetic diversity of prrs virus collected from air samples in four different regions of concentrated swine production during a high incidence season.	porcine reproductive and respiratory syndrome (prrs) is one of the most relevant swine diseases in the us, costing the industry millions of dollars per year. unfortunately, disease control is difficult because of the virus dynamics, as prrs virus (prrsv) can be transmitted by air between farms, especially, in regions with high density of swine operations. while long distance airborne transport of prrsv has been reported, there is little information regarding the dynamics of prrsv airborne challe ...	2014	25405592
comparison of noga endocardial mapping and cardiac magnetic resonance imaging for determining infarct size and infarct transmurality for intramyocardial injection therapy using experimental data.	we compared the accuracy of noga endocardial mapping for delineating transmural and non-transmural infarction to the results of cardiac magnetic resonance imaging (cmri) with late gadolinium enhancement (le) for guiding intramyocardial reparative substance delivery using data from experimental myocardial infarction studies.	2014	25409528
characterisation of pseudorabies virus in domestic pigs and wild boars in croatia.	serological data imply that pseudorabies (aujeszky's disease) is present in domestic pigs and wild boars in croatia. therefore, this study included testing of brain tissue samples collected from 200 domestic pigs and 105 wild boars originating from seventeen districts of croatia. the presence of pseudorabies virus (prv) dna was confirmed in samples originating from six domestic pigs (3%) and one wild boar (0.95%). positive samples were sequenced and analysed on the basis of a gc genome fragment. ...	2014	25410393
aberrant expression of mir-218 and mir-204 in human mesial temporal lobe epilepsy and hippocampal sclerosis-convergence on axonal guidance.	mesial temporal lobe epilepsy (mtle) is one of the most common types of the intractable epilepsies and is most often associated with hippocampal sclerosis (hs), which is characterized by pronounced loss of hippocampal pyramidal neurons. micrornas (mirnas) have been shown to be dysregulated in epilepsy and neurodegenerative diseases, and we hypothesized that mirnas could be involved in the pathogenesis of mtle and hs.	2014	25410734
domestic, peridomestic and wild hosts in the transmission of trypanosoma cruzi in the caatinga area colonised by triatoma brasiliensis.	the role played by different mammal species in the maintenance of trypanosoma cruzi is not constant and varies in time and place. this study aimed to characterise the importance of domestic, wild and peridomestic hosts in the transmission of t. cruzi in tauá, state of ceará, caatinga area, brazil, with an emphasis on those environments colonised by triatoma brasiliensis. direct parasitological examinations were performed on insects and mammals, serologic tests were performed on household and out ...	2014	25410992
comparison of the decomposition voc profile during winter and summer in a moist, mid-latitude (cfb) climate.	the investigation of volatile organic compounds (vocs) associated with decomposition is an emerging field in forensic taphonomy due to their importance in locating human remains using biological detectors such as insects and canines. a consistent decomposition voc profile has not yet been elucidated due to the intrinsic impact of the environment on the decomposition process in different climatic zones. the study of decomposition vocs has typically occurred during the warmer months to enable chem ...	2014	25412504
preliminary observations of arthropods associated with buried carrion on oahu.	several studies in hawaii have focused on arthropod succession and decomposition patterns of surface remains, but the current research presents the first study to focus on shallow burials in this context. three domestic pig carcasses (sus scrofa l.) were buried at the depths of 20-40 cm in silty clay loam soil on an exposed ridge on the leeward side of the volcanically formed koolau mountain range. one carcass was exhumed after 3 weeks, another after 6 weeks, and the last carcass was exhumed aft ...	2015	25413711
strong artificial selection in domestic mammals did not result in an increased recombination rate.	recombination rates vary in intensity and location at the species, individual, sex and chromosome levels. despite the fundamental biological importance of this process, the selective forces that operate to shape recombination rate and patterns are unclear. domestication offers a unique opportunity to study the interplay between recombination and selection. in domesticates, intense selection for particular traits is imposed on small populations over many generations, resulting in organisms that d ...	2014	25414125
natural and experimental hepatitis e virus genotype 3-infection in european wild boar is transmissible to domestic pigs.	hepatitis e virus (hev) is the causative agent of acute hepatitis e in humans in developing countries, but sporadic and autochthonous cases do also occur in industrialised countries. in europe, food-borne zoonotic transmission of genotype 3 (gt3) has been associated with domestic pig and wild boar. however, little is known about the course of hev infection in european wild boar and their role in hev transmission to domestic pigs. to investigate the transmissibility and pathogenesis of wild boar- ...	2014	25421429
cellular scaling rules for the brain of artiodactyla include a highly folded cortex with few neurons.	quantitative analysis of the cellular composition of rodent, primate, insectivore, and afrotherian brains has shown that non-neuronal scaling rules are similar across these mammalian orders that diverged about 95 million years ago, and therefore appear to be conserved in evolution, while neuronal scaling rules appear to be free to vary in a clade-specific manner. here we analyze the cellular scaling rules that apply to the brain of artiodactyls, a group within the order cetartiodactyla, believed ...	2014	25429261
african swine fever diagnosis adapted to tropical conditions by the use of dried-blood filter papers.	the performance of whatman 3-mm filter papers for the collection, drying, shipment and long-term storage of blood at ambient temperature, and for the detection of african swine fever virus and antibodies was assessed. conventional and real-time pcr, viral isolation and antibody detection by elisa were performed on paired samples (blood/tissue versus dried-blood 3-mm filter papers) collected from experimentally infected pigs and from farm pigs in madagascar and côte d'ivoire. 3-mm filter papers w ...	2016	25430732
detection of african swine fever antibodies in experimental and field samples from the russian federation: implications for control.	african swine fever (asf) re-entered in europe in 2007 by georgia rapidly affecting neighbouring countries. since then, asf has caused severe problems to the russian federation (rf) and spread to northern and western regions, including ukraine (2012 and 2014) and belarus (2013). at the beginning of 2014, dead wild boars were found in lithuania and poland. several outbreaks have been later notified in the european union(eu), affecting domestic pigs and wild boar of latvia, lithuania and poland, a ...	2016	25440300
submucosal endoscopy as an aid to full-thickness resection: pilot study in the porcine stomach.	endoscopic full-thickness resection (eftr) is commonly performed with laparoscopic assistance. submucosal endoscopy with full-thickness resection (seftr) is a new technique that combines submucosal endoscopy with the mucosal safety valve flap method to enable eftr.	2015	25440673
evaluation of the distribution of paclitaxel by immunohistochemistry and nuclear magnetic resonance spectroscopy after the application of a drug-eluting balloon in the porcine ureter.	the urothelium represents "the tightest and most impermeable barrier in the body." we investigated the distribution of paclitaxel (ptx) in the ureteral wall after the inflation of a paclitaxel-eluting balloon (peb) in an attempt to elucidate the possibility of clinical application of pebs in the ureter.	2015	25441059
changes to soil bacterial profiles as a result of sus scrofa domesticus decomposition.	the importance of cadaver decomposition knowledge for clandestine grave location cannot be over emphasised. notwithstanding this, only a limited understanding is available on the resulting soil microbial community dynamics. to address this paucity, a pig leg (sus scrofa domesticus; 5kg) was buried in freshly weighed (20kg) sandy loamy soil in a sealed microcosm (40cm height) in parallel with a soil only control. both microcosms were perforated nine times at equal distances and maintained outside ...	2014	25447182
a tourniquet assisted cardiopulmonary resuscitation augments myocardial perfusion in a porcine model of cardiac arrest.	during cardiopulmonary resuscitation (cpr), myocardial blood flow generated by chest compression rarely exceeds 35% of its normal level. cardiac output generated by chest compression decreases gradually with the prolongation of cardiac arrest and resuscitation. early studies have demonstrated that myocardial blood flow during cpr is largely dependent on peripheral vascular resistance. in this study, we investigated the effects of chest compression in combination with physical control of peripher ...	2015	25447436
molecular typing of streptococcus suis isolates from iberian pigs: a comparison with isolates from common intensively-reared commercial pig breeds.	the iberian pig (ip) is a traditional spanish breed variety of the domestic pig (sus scrofa domesticus) with high economic importance because of the value of the dry-cured products in national and international markets. the genetic characteristics of tonsillar and clinical streptococcus suis isolates from the ip maintained under extensive or intensive management conditions were investigated. s. suis isolates from ip pigs were compared with s. suis isolates from intensively-farmed pigs of common ...	2014	25458888
phenotypic and functional differentiation of porcine αβ t cells: current knowledge and available tools.	domestic pigs are considered as a valuable large animal model because of their close relation to humans in regard to anatomy, genetics and physiology. this includes their potential use as organ donors in xenotransplantation but also studies on various zoonotic infections affecting pigs and humans. such work also requires a thorough understanding of the porcine immune system which was partially hampered in the past by restrictions on available immunological tools compared to rodent models. howeve ...	2015	25466616
expression of the myosin heavy chain iib gene in porcine skeletal muscle: the role of the carg-box promoter response element.	due to its similarity to humans, the pig is increasingly being considered as a good animal model for studying a range of human diseases. despite their physiological similarities, differential expression of the myosin heavy chain (myhc) iib gene (myh4) exists in the skeletal muscles of these species, which is associated with a different muscle phenotype. the expression of different myhc isoforms is a critical determinant of the contractile and metabolic characteristics of the muscle fibre. we aim ...	2014	25469802
investigation into the epidemiology of african swine fever virus at the wildlife - domestic interface of the gorongosa national park, central mozambique.	an epidemiological study of african swine fever (asf) was conducted between march 2006 and september 2007 in a rural area adjacent to the gorongosa national park (gnp) located in the central mozambique. domestic pigs and warthogs were sampled to determine the prevalence of antibodies against asf virus and the salivary antigens of ornithodoros spp. ticks, while ticks collected from pig pens were tested for the presence of asfv. in addition, 310 framers were interviewed to gain a better understand ...	2016	25483914
endoscopic ultrasound-assisted direct peritoneal visualization with a small-caliber scope: a proof of concept study in a swine model.	laparoscopic and natural orifice transluminal endoscopic surgery techniques can diagnose peritoneal findings that suggest tumor cell dissemination. however, they have not been incorporated into routine practice, mainly owing to their complexity. to develop a minimally invasive endoscopic technique for the diagnosis of peritoneal findings, we conducted feasibility study using an acute swine model.	2014	25485270
[intramural chronotopography of depolarization of myocardium of heart ventricles of pig (sus scrofa domesticus)].	sequence of depolarization of myocardium of pig heart ventricles was studied by the method of multichannel synchronous cardioelectrotopography. there is established formation of areas of early depolarization in subendocardium of interventricular septum and in the base of left ventricle papillary muscles; of multiple foci--in the depth of walls; of areas of late depolarization--in subepicardium of the left ventricle dorsolateral side. as compared with other species of ungulate animals (reindeer a ...	2014	25486814
unravelling the complexity of domestication: a case study using morphometrics and ancient dna analyses of archaeological pigs from romania.	current evidence suggests that pigs were first domesticated in eastern anatolia during the ninth millennium cal bc before dispersing into europe with early neolithic farmers from the beginning of the seventh millennium. recent ancient dna (adna) research also indicates the incorporation of european wild boar into domestic stock during the neolithization process. in order to establish the timing of the arrival of domestic pigs into europe, and to test hypotheses regarding the role european wild b ...	0	25487340
comparison of osteoconductive properties of three different β-tricalcium phosphate graft materials: a pilot histomorphometric study in a pig model.	the aim of this study was to compare the de novo bone formation ability and osteoconductive effects of three different β-tricalcium phosphate (β-tcp) graft materials. the micro-architectural parameters of the newly formed bone tissues were also compared among the different graft materials.	2015	25491275
hepadnavirus detected in bile and liver samples from domestic pigs of commercial abattoirs.	preliminary studies showed the prevalence of a virus similar to human hepatitis b virus (hbv-like) in swine from farms in china and the molecular evidence of hepadnavirus infection in domestic pigs herds in brazil. in this study, we genetically characterize the swine hepadnavirus strains in swine from slaughterhouses located in certified abattoirs from rio de janeiro state, brazil and evaluate its hepatotropic potential.	2014	25495746
activation of intervertebral disc cells by co-culture with notochordal cells, conditioned medium and hypoxia.	notochordal cells (nc) remain in the focus of research for regenerative therapy for the degenerated intervertebral disc (ivd) due to their progenitor status. recent findings suggested their regenerative action on more mature disc cells, presumably by the secretion of specific factors, which has been described as notochordal cell conditioned medium (nccm). the aim of this study was to determine nc culture conditions (2d/3d, fetal calf serum, oxygen level) that lead to significant ivd cell activat ...	2014	25496082
the progressive adaptation of a georgian isolate of african swine fever virus to vero cells leads to a gradual attenuation of virulence in swine corresponding to major modifications of the viral genome.	african swine fever virus (asfv) causes a contagious and often lethal disease of feral and domestic swine. experimental vaccines derived from naturally occurring, genetically modified, or cell culture-adapted asfv have been evaluated, but no commercial vaccine is available to control african swine fever (asf). we report here the genotypic and phenotypic analysis of viruses obtained at different passages during the process of adaptation of a virulent asfv field isolate from the republic of georgi ...	2015	25505073
two-in-one aortic valve sizing and valvuloplasty conductance balloon catheter.	inaccurate aortic valve sizing and selection is linked to paravalvular leakage in transcatheter aortic valve replacement (tavr). here, a novel sizing valvuloplasty conductance balloon (svcb) catheter is shown to be accurate, reproducible, unbiased, and provides real-time tool for aortic valve sizing that fits within the standard valvuloplasty procedure.	2015	25510238
studying classical swine fever virus: making the best of a bad virus.	classical swine fever (csf) is a highly contagious and often fatal disease that affects domestic pigs and wild boars. outbreak of csf can cause heavy economic losses to the pig industry. the strategies to prevent, control and eradicate csf disease are based on containing the disease through a systematic prophylactic vaccination policy and a non-vaccination stamping-out policy. the quest for prevention, control and eradication of csf has moved research forward in academia and industry, and has pr ...	2015	25510481
detection of a novel single nucleotide polymorphism and imprinted status analysis of the ras protein-specific guanine nucleotide-releasing factor 1 gene in domestic pigs.	the aim of this study was to determine the imprinting status of the ras protein-specific guanine nucleotide-releasing factor 1 (rasgrf1) gene in domestic pigs. in this study, a 228-bp partial sequence located in exon 14 and a 193-bp partial sequence located in exon 1 of the rasgrf1 gene in domestic pigs were obtained. a novel single nucleotide polymorphism, a g/a transition, was identified in rasgrf1 exon 14, and then the reciprocal berkshire x wannan black f1 hybrid model and the reverse transc ...	2014	25511042
utility of cell viability assays for use with ex vivo vocal fold epithelial tissue.	ex vivo models are routinely used to investigate the barrier function of the vocal fold epithelium. however, there are limited reports on assays that can be used to investigate the effect of clinically relevant challenges on vocal fold epithelial tissue viability. our objective was to determine the utility of two assays routinely used in cell culture-a cellular metabolic activity assay and a cell membrane integrity assay-to investigate the viability of ex vivo porcine vocal fold epithelium.	2014	25511412
pulsatile venoarterial perfusion using a novel synchronized cardiac assist device augments coronary artery blood flow during ventricular fibrillation.	patients with cardiogenic shock have a very high mortality. here we report the first use of a percutaneous pulsatile cardiac assist device, based on a diagonal pump synchronized with the heart cycle by means of an electrocardiographic signal in adult pigs. eight domestic pigs underwent mandatory ventilation. during sinus rhythm, there were no differences between pulsatile and nonpulsatile perfusion with regard to pulmonary artery pressure, pulmonary wedge pressure, central venous pressure, mean ...	2015	25511441
a large animal model of spinal muscular atrophy and correction of phenotype.	spinal muscular atrophy (sma) is caused by reduced levels of survival motor neuron (smn) protein, which results in motoneuron loss. therapeutic strategies to increase smn levels including drug compounds, antisense oligonucleotides, and scaav9 gene therapy have proved effective in mice. we wished to determine whether reduction of smn in postnatal motoneurons resulted in sma in a large animal model, whether sma could be corrected after development of muscle weakness, and the response of clinically ...	2015	25516063
assessment of gel-coated delayed self-gripping mesh.	mesh hernia repair is one of the most frequently performed procedures in general surgery. recently, use of the self-gripping mesh demonstrated a beneficial effect on postoperative pain in inguinal hernia repairs. however, in intra-abdominal placement, the use of this novel mesh requires greater laparoscopic skill and dexterity because of the mesh's tendency to fold and adhere to itself and to any surrounding tissues. we hypothesized that gel-like coverage of a self-gripping mesh with a water-sol ...	0	25516704
biomechanical evaluation of three fixation modalities for preperitoneal inguinal hernia repair: a 24-hour postoperative study in pigs.	tacks and sutures ensure a strong fixation of meshes, but they can be associated with pain and discomfort. less invasive methods are now available. three fixation modalities were compared: the progrip™ laparoscopic self-fixating mesh; the fibrin glue tisseel™ with bard™ soft mesh; and the sorbafix™ absorbable fixation system with bard™ soft mesh.	2014	25525396
sodium nitroprusside-enhanced cardiopulmonary resuscitation facilitates intra-arrest therapeutic hypothermia in a porcine model of prolonged ventricular fibrillation.	the aim of this study was to assess the effect of sodium nitroprusside-enhanced cardiopulmonary resuscitation on heat exchange during surface cooling. we hypothesized that sodium nitroprusside-enhanced cardiopulmonary resuscitation would decrease the time required to reach brain temperature less than 35°c compared to active compression-decompression plus impedance threshold device cardiopulmonary resuscitation alone, in the setting of intra-cardiopulmonary resuscitation cooling. we further hypot ...	2015	25525755
gastrointestinal acute radiation syndrome in göttingen minipigs (sus scrofa domestica).	in the absence of supportive care, exposing göttingen minipigs to γ-radiation doses of less than 2 gy achieves lethality due to hematopoietic acute radiation syndrome. doses of 2 to 5 gy are associated with an accelerated hematopoietic syndrome, characterized by villus blunting and fusion, the beginning of sepsis, and a mild transient reduction in plasma citrulline concentration. we exposed male göttingen minipigs (age, 5 mo; weight, 9 to 11 kg) to γ-radiation doses of 5 to 12 gy (total body; (6 ...	2014	25527026
novel technique for retroperitoneal implantation of telemetry transmitters for physiologic monitoring in göttingen minipigs (sus scrofa domesticus).	telemetric monitoring of physiologic parameters in animal models is a critical component of chemical and biologic agent studies. the long-term collection of neurobehavioral and other physiologic data can require larger telemetry devices. furthermore, such devices must be implanted in a location that is safe, well-tolerated, and functional. göttingen minipigs (sus scrofa domesticus) present an ideal large animal model for chemical agent studies due to their relatively small size, characterized he ...	0	25527027
brucella suis infection in domestic pigs in sardinia (italy).	during a 4-year (2007-2010) survey, the presence of brucella suis infection in domestic pigs in sardinia was investigated. serum samples were collected from breeding pigs located on 108 commercial farms with documented reproductive problems and analysed using the rose bengal (rbt) and complement fixation (cft) tests for screening and confirmation of brucella, respectively. of the 1251 serum samples analysed by rbt, 406 sera, originating from 36 farms, were positive for b. suis. cft was positive ...	2015	25530227
high-resolution melting of 12s rrna and cytochrome b dna sequences for discrimination of species within distinct european animal families.	the cheap and easy identification of species is necessary within multiple fields of molecular biology. the use of high-resolution melting (hrm) of dna provides a fast closed-tube method for analysis of the sequence composition of the mitochondrial genes 12s rrna and cytochrome b. we investigated the potential use of hrm for species identification within eleven different animal groups commonly found in europe by animal-group-specific dna amplification followed by dna melting. influence factors as ...	2014	25531439
pathological and molecular diagnosis of the 2013 african swine fever outbreak in lusaka, zambia.	african swine fever (asf) is a highly contagious and fatal hemorrhagic viral disease of domestic pigs. the disease is widespread in sub-saharan africa and has repeatedly been introduced into other continents. the current study describes the diagnostic investigations of a hemorrhagic disease that was reported in pigs in lusaka (october 2013), zambia. necropsy, histopathology, and molecular diagnosis using polymerase chain reaction and sequence analysis confirmed the disease to be asf. the sequenc ...	2015	25535151
ccaat-enhancer binding protein (c/ebp) β regulates insulin-like growth factor (igf) 1 expression in porcine liver during prenatal and postnatal development.	igf1 expression regulation attracts numerous interests because of its important role during mammalian growth and development. domestic pig can be used as a valuable animal model to investigate human development since they share the high similarity in general physiology and metabolism. in this study, we examined the expression pattern of igf1 and found it associated with liver c/ebp β expression pattern in porcine liver during embryonic and postnatal development. both igf1 and c/ebp β expression ...	2015	25543525
a pilot experimental study of a catheter to facilitate treatment for penetrating cardiac injury.	penetrating heart injuries result in high mortality. we designed a new catheter to facilitate the treatment of penetrating cardiac injuries and provide more effective initial bleeding control and fluid replacement.	2014	25552144
redox state of flavin adenine dinucleotide drives substrate binding and product release in escherichia coli succinate dehydrogenase.	the complex ii family of enzymes, comprising respiratory succinate dehydrogenases and fumarate reductases, catalyzes reversible interconversion of succinate and fumarate. in contrast to the covalent flavin adenine dinucleotide (fad) cofactor assembled in these enzymes, soluble fumarate reductases (e.g., those from shewanella frigidimarina) that assemble a noncovalent fad cannot catalyze succinate oxidation but retain the ability to reduce fumarate. in this study, an sdha-h45a variant that elimin ...	2015	25569225
constant hepatitis e virus (hev) circulation in wild boar and red deer in spain: an increasing concern source of hev zoonotic transmission.	hepatitis e is a viral zoonosis that affects multiple hosts. the complete dynamics of infection in wildlife are still unknown, but the previous fact facilitates the maintenance and circulation of the virus, posing a risk to human health in the case of meat consumption from susceptible animals. in spain, it has been shown how domestic pigs, cattle and wildlife (i.e. wild boar and red deer) clearly interact in hunting farms, generating a complex epidemiological situation in terms of interspecies p ...	2016	25571944
profiles of reproductive hormone in the microminipig during the normal estrous cycle.	we investigated changes in the peripheral plasma concentrations of ovarian steroids and gonadotropins throughout the estrous cycle of the microminipig (mmpig), a novel experimental animal model. the mean (± standard error of the mean (sem)) duration of estrous and the estrous cycle in seven animals was 66.0 ± 5.2 h and 20.6 ± 0.3 days, respectively. a luteinizing hormone (lh) surge was observed 16.5 ± 6.5 h after the onset of estrous in six out of the seven animals. the lh peak and the duration ...	2015	25600524
hybrid origin of european commercial pigs examined by an in-depth haplotype analysis on chromosome 1.	although all farm animals have an original source of domestication, a large variety of modern breeds exist that are phenotypically highly distinct from the ancestral wild population. this phenomenon can be the result of artificial selection or gene flow from other sources into the domesticated population. the eurasian wild boar (sus scrofa) has been domesticated at least twice in two geographically distinct regions during the neolithic revolution when hunting shifted to farming. prior to the est ...	2015	25601878
light microscopy and scanning electron microscopy study on microstructure of gallbladder mucosa in pig.	the present light microscopy (lm) and scanning electron microscopy (sem) studies on porcine gallbladder mucosa provide a description of the microstructures of great functional importance such as mucosal folds, the epithelium, glands, and lymphatic nodules. the results showed the regional structural differences of the porcine gallbladder wall. depending on the part of the gallbladder, three types of mucosal structures were described: simple and branched folds and mucosal crypts. an important stru ...	2015	25604381
liposarcoma in animals: literature review and case report in a domestic pig (sus scrofa).	liposarcomas are malignant tumors of adipocytes. the current report describes a liposarcoma in a 2.5-year-old, mixed-breed commercial sow that was detected during meat inspection. on gross examination, a firm, whitish, multinodular, 20 cm ×10 cm mass was observed in the perirenal area along with smaller nodules multifocally scattered within the renal parenchyma. histological examination revealed an anaplastic sarcoma with clear intracytoplasmic lipidic vacuoles that were positive for sudan black ...	2015	25613042
bat and pig ifn-induced transmembrane protein 3 restrict cell entry by influenza virus and lyssaviruses.	ifn-induced transmembrane protein 3 (ifitm3) is a restriction factor that blocks cytosolic entry of numerous viruses that utilize acidic endosomal entry pathways. in humans and mice, ifitm3 limits influenza-induced morbidity and mortality. although many ifitm3-sensitive viruses are zoonotic, whether ifitms function as antiviral restriction factors in mammalian species other than humans and mice is unknown. here, ifitm3 orthologues in the microbat (myotis myotis) and pig (sus scrofa domesticus) w ...	2015	25614588
first finding of trichinella pseudospiralis in the neotropical region.	prior to this study, only encapsulated species of trichinella had been found in south america, i.e., t. spiralis and t. patagoniensis. here we report the molecular identification of a non-encapsulated isolate of trichinella from a domestic pig in argentina. the multiplex pcr technique and the analysis of mitochondrial and nuclear dna sequences revealed that it belongs to t. pseudospiralis, which parasitises birds and mammals from australian, nearctic, and palaearctic regions. interestingly, the ...	2015	25620294
adaptation and possible ancient interspecies introgression in pigs identified by whole-genome sequencing.	domestic pigs have evolved genetic adaptations to their local environmental conditions, such as cold and hot climates. we sequenced the genomes of 69 pigs from 15 geographically divergent locations in china and detected 41 million variants, of which 21 million were absent from the dbsnp database. in a genome-wide scan, we identified a set of loci that likely have a role in regional adaptations to high- and low-latitude environments within china. intriguingly, we found an exceptionally large (14- ...	2015	25621459
quantitative characterization of endothelial cell morphologies depending on shear stress in different blood vessels of domestic pigs using a focused ion beam and high resolution scanning electron microscopy (fib-sem).	microstructured surfaces mimicking the endothelial cell (ec) morphology is a new approach to improve the blood compatibility of synthetic vascular grafts. the ecs are capable of changing their shapes depending on different shear conditions. however, the quantitative correlation between ec morphology and shear stress has not yet been investigated statistically. the aim of this study was to quantitatively investigate the morphology of ecs in dependence on the shear stress. blood flow rates in diff ...	2015	25622890
differential diptera succession patterns onto partially burned and unburned pig carrion in southeastern brazil.	in the present contribution we compared the entomological succession pattern of a burned carcass with that of an unburned one. for that, we used domestic pig carcasses and focused on calliphoridae, muscidae and sarcophagidae flies, because they are the ones most commonly used in postmortem interval estimates. adult and immature flies were collected daily. a total of 27 species and 2,498 specimens were collected, 1,295 specimens of 26 species from the partially burned carcass and 1,203 specimens ...	2014	25627597
adipose tissue remodeling in a novel domestic porcine model of diet-induced obesity.	to establish and characterize a novel domestic porcine model of obesity.	2015	25627626
comparison of pathogenicity of highly pathogenic porcine reproductive and respiratory syndrome virus between wild and domestic pigs.	the objective of this study was to compare the pathogenicity of highly pathogenic porcine reproductive and respiratory syndrome virus (hp-prrsv) infection between wild and domestic pigs based on clinical, immunological, and pathological evaluation. upon challenge with hp-prrsv, five wild pigs died compared to none of the domestic. anti-prrsv antibody titers were significantly (p < 0.05) higher in wild hp-prrsv-infected pigs versus the domestic hp-prrsv-infected pigs at 21 days post inoculation ( ...	2015	25634036
epidemiology of african swine fever in poland since the detection of the first case.	the purpose of this paper is to provide characteristics of the spread of african swine fever (asf) in poland from february to august, 2014. the samples from dead wild boar and domestic pigs were submitted to the national veterinary research institute, national reference laboratory for asf in pulawy, poland, for testing by pcr and elisa methods. in the studied period, fourteen cases of asf in wild boar and two outbreaks in backyard pigs were confirmed. in addition to the results of laboratory tes ...	2014	25638980
inhibition of nitric oxide synthases, but not inducible nitric oxide synthase, selectively worsens left ventricular function after successful resuscitation from cardiac arrest in swine.	nitric oxide (no) is a critical regulator of vascular tone and signal transduction in the cardiovascular system. no is synthesized by three unique enzymes (nitric oxide synthases [nos]): endothelial and neuronal nos, both constitutively expressed, and inducible nos (inos), which is induced by proinflammatory stimuli and subsequently produces a burst of no. no has been implicated as both an injurious and a beneficial mediator after cardiac arrest and resuscitation. a previous study in swine found ...	2015	25639298
phenotype and animal domestication: a study of dental variation between domestic, wild, captive, hybrid and insular sus scrofa.	identifying the phenotypic responses to domestication remains a long-standing and important question for researchers studying its early history. the great diversity in domestic animals and plants that exists today bears testament to the profound changes that domestication has induced in their ancestral wild forms over the last millennia. domestication is a complex evolutionary process in which wild organisms are moved to new anthropogenic environments. although modern genetics are significantly ...	2015	25648385
domestic pigs' (sus scrofa domestica) use of direct and indirect visual and auditory cues in an object choice task.	recently, foraging strategies have been linked to the ability to use indirect visual information. more selective feeders should express a higher aversion against losses compared to non-selective feeders and should therefore be more prone to avoid empty food locations. to extend these findings, in this study, we present a series of studies investigating the use of direct and indirect visual and auditory information by an omnivorous but selective feeder-the domestic pig. subjects had to choose bet ...	2015	25650328
pharmacokinetics of sustained-release and transdermal buprenorphine in göttingen minipigs (sus scrofa domestica).	the opioid buprenorphine has been shown to provide adequate postoperative analgesia in both companion and laboratory animals. however, its use is still hindered by the need for multiple parenteral injections to achieve continuous analgesia. the purpose of the current study was to conduct a pharmacokinetic analysis of 2 new long-acting formulations of buprenorphine-an injectable sustained-release buprenorphine (srb) and a transdermal buprenorphine (tdb) patch-in healthy göttingen minipigs by usin ...	0	25650977
gene coexpression networks reveal key drivers of phenotypic divergence in porcine muscle.	domestication of the wild pig has led to obese and lean phenotype breeds, and evolutionary genome research has sought to identify the regulatory mechanisms underlying this phenotypic diversity. however, revealing the molecular mechanisms underlying muscle phenotype variation based on differentially expressed genes has proved to be difficult. to characterize the mechanisms regulating muscle phenotype variation under artificial selection, we aimed to provide an integrated view of genome organizati ...	2015	25651817
mechanism by which shock wave lithotripsy can promote formation of human calcium phosphate stones.	human stone calcium phosphate (cap) content correlates with higher urine cap supersaturation (ss) and urine ph as well as with the number of shock wave lithotripsy (swl) treatments. swl does damage medullary collecting ducts and vasa recta, sites for urine ph regulation. we tested the hypothesis that swl raises urine ph and therefore cap ss, resulting in cap nucleation and tubular plugging. the left kidney (t) of nine farm pigs was treated with swl, and metabolic studies were performed using bil ...	2015	25656372
the effects of hemorrhagic shock secondary to hepatectomy in a swine model.	ischemia-reperfusion injury caused by severe hemorrhagic shock and subsequent resuscitation leads to deterioration of hepatic homeostasis and possibly to liver failure. the present study focuses on determining whether there is a different biological response to hemorrhagic shock by different sources of hemorrhage, hepatic hemorrhage (hh) versus peripheral hemorrhage.	2015	25659614
genetic variation of the east balkan swine (sus scrofa) in bulgaria, revealed by mitochondrial dna and y chromosomal dna.	east balkan swine (ebs) sus scrofa is the only aboriginal domesticated pig breed in bulgaria and is distributed on the western coast of the black sea in bulgaria. to reveal the breed's genetic characteristics, we analysed mitochondrial dna (mtdna) and y chromosomal dna sequences of ebs in bulgaria. nucleotide diversity (πn ) of the mtdna control region, including two newly found haplotypes, in 54 ebs was higher (0.014 ± 0.007) compared with that of european (0.005 ± 0.003) and asian (0.006 ± 0.0 ...	2015	25662601
detection of a new cluster of porcine circovirus type 2b strains in domestic pigs in germany.	pcv2 can be divided into three different genotypes: pcv2a, pcv2b and pcv2c. since 2004/2005 pcv2b has become the predominant genotype in the domestic pig population worldwide. in the years 2010 and 2012 pcv2b mutant strains (mpcv2), classified as pcv2b-1c strains, were detected in porcine circovirus diseases (pcvd) affected pigs in china and the united states, respectively. within one year (april 2013-april 2014) newly emerging mpcv2 strains were isolated in seven german pig farms routinely vacc ...	2015	25669598
comparison of electrical current and ultrasonic device for incision of the septum of the pharyngoesophageal diverticulum in a pig model.	complications of endoscopic treatment of pharyngoesophageal diverticulum (ped) such as bleeding and perforation occur in up to 15 % of cases. our objective was to compare incision of the ped septum using the ultrasonic device (group h) with incision using electrical current (group m) in a pig model.	2015	25669640
qtl for seed protein and amino acids in the benning × danbaekkong soybean population.	we identified qtl associated with protein and amino acids in a soybean mapping population that was grown in five environments. these qtl could be used in mas to improve these traits. soybean, rather than nitrogen-containing forages, is the primary source of quality protein in feed formulations for domestic swine, poultry, and dairy industries. as a sole dietary source of protein, soybean is deficient in the amino acids lysine (lys), threonine (thr), methionine (met), and cysteine (cys). increasi ...	2015	25673144
domestic pigs are susceptible to infection with influenza b viruses.	influenza b virus (ibv) causes seasonal epidemics in humans. although ibv has been isolated from seals, humans are considered the primary host and reservoir of this important pathogen. it is unclear whether other animal species can support the replication of ibv and serve as a reservoir. swine are naturally infected with both influenza a and c viruses. to determine the susceptibility of pigs to ibv infection, we conducted a serological survey for u.s. midwest domestic swine herds from 2010 to 20 ...	2015	25673727
exposure of feral swine (sus scrofa) in the united states to selected pathogens.	feral swine (sus scrofa) are widely distributed in the united states. in 2011 and 2012, serum samples and tonsils were recovered from 162 and 37 feral swine, respectively, in the us to evaluate exposure to important swine endemic pathogens. antibodies against porcine reproductive and respiratory syndrome virus (prrsv) and porcine circovirus type 2 (pcv2) were found in 2.5% and 25.3% of tested sera, respectively. positive serological reactions against mycoplasma hyopneumoniae and actinobacillus p ...	0	25673913
catheter based selective hypothermia reduces stroke volume during focal cerebral ischemia in swine.	total body hypothermia is an established neuroprotectant in global cerebral ischemia. the role of hypothermia in acute ischemic stroke remains uncertain. selective application of hypothermia to a region of focal ischemia may provide similar protection with more rapid cooling and elimination of systemic side effects. we studied the effect of selective endovascular cooling in a focal stroke model in adult domestic swine.	2016	25676148
modeling cardiac arrest and resuscitation in the domestic pig.	cardiac arrest remains a leading cause of death and permanent disability worldwide. although many victims are initially resuscitated, they often succumb to the extensive ischemia-reperfusion injury inflicted on the internal organs, especially the brain. cardiac arrest initiates a complex cellular injury cascade encompassing reactive oxygen and nitrogen species, ca(2+) overload, atp depletion, pro- and anti-apoptotic proteins, mitochondrial dysfunction, and neuronal glutamate excitotoxity, which ...	2015	25685718
immunization of african indigenous pigs with attenuated genotype i african swine fever virus ourt88/3 induces protection against challenge with virulent strains of genotype i.	the attenuated african swine fever virus genotype i strain ourt88/3 has previously been shown to induce protection of european breeds of domestic pigs against challenge with virulent isolates. to determine whether protective immune responses could also be induced in indigenous breeds of pigs from the kinshassa region in democratic republic of congo, we immunized a group of eight pigs with ourt88/3 strain and challenged the pigs 3 weeks later with virulent genotype i strain ourt88/1. four of the ...	2016	25691347
a taphonomic study exploring the differences in decomposition rate and manner between frozen and never frozen domestic pigs (sus scrofa).	this research examined differences in decomposition rate and manner of domestic pig subjects (sus scrofa) in never frozen (control) and previously frozen (experimental) research conditions. eight control and experimental subjects were placed in an identical outdoor research environment. daily quantitative and qualitative measurements were collected: abdominal circumference, total body score (tbs), temperature, photographs, descriptive decomposition stages, and visual observations. field necropsi ...	2015	25693630
randomized trial on the physiologic impact of notes.	our previous work revealed significantly less acidosis in swine undergoing natural orifice translumenal endoscopic surgery (notes) using endoscopic air insufflation than swine undergoing standard laparoscopy. we wanted to evaluate the differential effects of co2 versus intra-abdominal pressure as source for this finding. in addition, we investigated the endocrine stress response between swine undergoing notes peritoneoscopy with co2 insufflation and animals undergoing standard diagnostic laparos ...	2015	25698397
cardiac remodeling in a new pig model of chronic heart failure: assessment of left ventricular functional, metabolic, and structural changes using pet, ct, and echocardiography.	large animal models are needed to study disease mechanisms in heart failure (hf). in the present study we characterized the functional, metabolic, and structural changes of myocardium in a novel pig model of chronic myocardial infarction (mi) by using multimodality imaging and histology.	2015	25698475
epidemiology and molecular characterization of cryptosporidium spp. in humans, wild primates, and domesticated animals in the greater gombe ecosystem, tanzania.	cryptosporidium is an important zoonotic parasite globally. few studies have examined the ecology and epidemiology of this pathogen in rural tropical systems characterized by high rates of overlap among humans, domesticated animals, and wildlife. we investigated risk factors for cryptosporidium infection and assessed cross-species transmission potential among people, non-human primates, and domestic animals in the gombe ecosystem, kigoma district, tanzania. a cross-sectional survey was designed ...	2015	25700265