Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| effect of naproxen on tail fin regeneration in teleost. | in the present investigation we studied the effect of naproxen, a non-steroidal anti-inflammatory drug used for the treatment of rheumatic disease, on the synthesis of the lepidotrichial matrix of the tail fin of the teleost fish cyprinus carpio (carp) during the regeneration process. the lepidotrichial synthesis was observed by standard and polarized light microscopy and by transmission electron microscopy. in general, naproxen at the dose used in the present study did not affect the organizati ... | 2004 | 15311675 |
| cloning and characterisation of natural resistance associated macrophage protein (nramp) cdna from red sea bream (pagrus major). | nramp (natural resistance associated macrophage protein) controls aspects of innate resistance to intracellular parasites. its function is to enhance the ability of macrophages to kill pathogens. however, little is known about the structure and function of nramp in lower vertebrates such as teleosts. we have recently isolated a cdna encoding nramp from spleen of red sea bream (pagrus major). the full-length cdna of the nramp is 4709 bp in length, including 197 bp 5'-terminal untranslated region ... | 2004 | 15312657 |
| in situ and laboratory bioassays to evaluate the impact of effluent discharges on receiving aquatic ecosystems. | effluents are a main source of direct and often continuous input of pollutants into aquatic ecosystems with long-term implications on ecosystem functioning. therefore, the study of the effects of effluent exposure on organisms, populations or communities within the framework of impact assessment has a high ecological relevance. the aim of this study was to assess the toxicological impact of two effluents, one household wastewater treatment effluent (effluent 1) and one industrial effluent (efflu ... | 2004 | 15312937 |
| nitrite toxicity in indian major carps: sublethal effect on selected enzymes in fingerlings of catla catla, labeo rohita and cirrhinus mrigala. | the effects of 96-h sublethal exposure of nitrite (1, 2, 4, 8 and 10.4 mg l(-1)) on selected enzymatic activities in serum and tissues of fingerlings of catla (catla catla), rohu (labeo rohita) and mrigal (cirrhinus mrigala) were studied for the first time in these species. all three species responded almost identically to nitrite exposure. with increasing nitrite concentration, reduction in activities was observed in acetylcholinesterase (ache) in brain and liver; alkaline phosphatase (alp) in ... | 2004 | 15313441 |
| visible light affects chemiluminescence of carp (cyprinus carpio) blood leukocytes. | visible light irradiations at doses of 5 and 12 j/cm(2) were applied to carp buffy coat leukocytes. | 2004 | 15315734 |
| designing of novel carbonic anhydrase inhibitors and activators. | carbonic anhydrases (cas, ec 4.2.1.1) are wide spread enzymes, present in mammals in at least 14 different isoforms: some of these isozymes are cytosolic (ca i, ca ii, ca iii, ca vii), while others are membrane-bound (ca iv, ca ix, ca xii and ca xiv); ca v is mitochondrial, and ca vi is secreted in the saliva. three acatalytic forms are also known (carp viii, carp x and carp xi). several important physiological and physio-pathological functions are played by many ca isozymes, which are strongly ... | 2004 | 15320807 |
| stabilisation of scoliosis in two koi (cyprinus carpio). | two koi (cyprinus carpio) from the same pond developed similar lesions of scoliosis. radiographic examinations showed that their spines had become malaligned as a result of vertebral compression fractures involving t14 to t16. the vertebrae in both fish were stabilised with screws, k-wire and polymethylmethacrylate. they both appeared to improve after surgery, but they began to decline and died within three months. a postmortem examination revealed multi-organ inflammation that was not associate ... | 2004 | 15328741 |
| designing of novel carbonic anhydrase inhibitors and activators. | carbonic anhydrases (cas, ec 4.2.1.1) are wide spread enzymes, present in mammals in at least 14 different isoforms: some of these isozymes are cytosolic (ca i, ca ii, ca iii, ca vii), while others are membrane-bound (ca iv, ca ix, ca xii and ca xiv); ca v is mitochondrial, and ca vi is secreted in the saliva. three acatalytic forms are also known (carp viii, carp x and carp xi). several important physiological and physio-pathological functions are played by many ca isozymes, which are strongly ... | 2004 | 15328829 |
| dietary requirement of tryptophan for growth and survival of the indian major carp, cirrhinus mrigala (hamilton-buchanan) fry. | the experimental diets (40% protein) contained graded levels of tryptophan (0.60, 0.75, 1.00, 1.25, 1.50, 1.75%) of dietary protein. each test diet was fed to triplicate groups of mrigal fry twice daily at 10% of the total biomass for 8 weeks. the dietary tryptophan requirement was estimated by plotting weight gain against dietary levels of tryptophan using two separate regression equations, the point of intersection of two equations was taken as optimum level, which occurred at 0.48% of the die ... | 2003 | 15332510 |
| evolution of vertebrate genes related to prion and shadoo proteins--clues from comparative genomic analysis. | recent findings of new genes in fish related to the prion protein (prp) gene prnp, including our recent report of sprn coding for shadoo (sho) protein found also in mammals, raise issues of their function and evolution. here we report additional novel fish genes found in public databases, including a duplicated sprn gene, sprnb, in fugu, tetraodon, carp, and zebrafish encoding the sho2 protein, and we use comparative genomic analysis to analyze the evolutionary relationships and to infer evoluti ... | 2004 | 15342797 |
| [genetic analysis on diversity of reproductive modes of silver crucian carp inferred by transferrin and isozyme markers]. | silver crucian carp (carassius auratus gibelio) has been known for its gynogenetic reproduction. in this paper, eggs from clone f of gynogenetic silver crucian carp were inseminated respectively by sperms of clone d, clone a and common carp, and polymorphic patterns of transferrin and isozymes of the produced three offspring fd, fa and fl were studied comparatively to explore the diversity in reproductive modes of silver crucian carp. as control, zymograms of fl progenies exhibited a maternally ... | 2002 | 15346982 |
| the distribution of osteocalcin, degree of mineralization, and mechanical properties along the length of cyprinus carpio rib bone. | this study examined the spatial distribution of selected biochemical and mechanical properties along the length of carp rib bone. carp rib bone was chosen because of its unusually high osteocalcin content relative to other extractable proteins. the amount of osteocalcin was significantly lower (p<0.01) at the most distal section, relative to all other sections. the amount of phosphate (p<0.05) and the elastic modulus in the longitudinal plane (p<0.0001) were found to be significantly higher in t ... | 2001 | 15348241 |
| xenobiotic substances such as pcb mixtures (aroclor 1254) and tbt can influence swimming behavior and biotransformation activity (gst) of carp (cyprinus carpio). | different groups of carp were treated with polychlorinated biphenyl (pcb) or tributyltin (tbt), and possible effects of the chemicals on the swimming behavior of the carp were examined using the behavioquant system. by evaluating quantitative behavioral parameters of the animals, it became evident that exposure to high concentrations of chemicals (organotin, 7 microg l(-1), or polychlorinated biphenyl, 22 microg l(-1)) severely affected the carp, causing a significant change in their swimming sp ... | 2004 | 15352262 |
| dietary accumulation and metabolism of polybrominated diphenyl ethers by juvenile carp (cyprinus carpio). | polybrominated diphenyl ethers (pbdes) are hydrophobic organic contaminants with properties and nomenclature similar to polychlorinated biphenyls (pcbs). while much information is available on the bioaccumulation and pharamcokinetics of pcbs, little information is available on pbdes. in this study, juvenile carp were exposed to a diet spiked with a cocktail of four bde congeners (2,4,4'-tribromode [bde 28], 2,2',4,4'-tetrabromode [bde 47], 2,2',4,4',5-pentabromode [bde 99], and 2,2',4,4',5,5'hex ... | 2004 | 15352483 |
| some immune parameters in carp cyprinus carpio susceptible and resistant to the haemoflagellate trypanoplasma borreli. | the present study addresses aspects of the (specific) immune response of carp to the haemoflagellate trypanoplasma borreli. sera of resistant carp contained antibodies, which agglutinated the flagellates in vitro. when flagellates were incubated in fish sera from resistant carp, binding of antibodies to flagellates could be demonstrated by flow cytometry, and t. borreli were effectively killed. heat-treatment of the sera prevented killing, indicating that complement activation is important for t ... | 2004 | 15352523 |
| homing of gyrodactylus salaris and g. derjavini (monogenea) on different hosts and response post-attachment. | in natural european waters, the congeneric monogeneans gyrodactylus derjavini mikailov, 1975 and g. salaris malmberg, 1957 are primarily found on brown trout salmo trutta l. and atlantic salmon salmo salar l., respectively. interestingly, rainbow trout, oncorhynchus mykiss (walbaum), originating from north america, is as susceptible as brown trout to g. derjavini. however, the mechanisms involved in this host specificity are poorly understood but may include behavioural, mechanical and chemical ... | 2004 | 15357405 |
| determination of polychlorinated dibenzo-p-dioxins and dibenzofurans and comparison of extraction methods for edible freshwater fish and frogs in south korea by using a high-resolution gc/ms. | three different extraction methods were evaluated: soxhlet extraction, accelerated solvent extractor extraction and alkali digestion with high-resolution gas chromatography/high-resolution mass spectrometry, for the analysis of edible freshwater fish and frogs. the recovery efficiencies for the different extraction methods were also compared. using these extraction methods, the concentrations and spatial distributions of these compounds in freshwater fish and frogs (n = 99), collected from 31 lo ... | 2004 | 15370843 |
| characterization of retinoic acid neuromodulation in the carp retina. | visual sensation in vertebrates starts with the isomerization of 11-cis retinaldehyde into all-trans retinaldehyde. aldehyde dehydrogenases, present in the pigment epithelium and some retinal cells, convert all-trans retinaldehyde into all-trans retinoic acid (at-ra). evidence in the retina and the hippocampus has accumulated, showing that at-ra, besides being a morphogenetic factor, also acts as a neuromodulator. in mature retina, at-ra affects visual processing by acting on gap junctional cond ... | 2004 | 15378613 |
| immunotoxicity of pentachlorophenol on macrophage immunity and igm secretion of the crucian carp (carassius auratus). | 2004 | 15386086 | |
| immunology-related perturbations induced by copper and chitosan in carp (cyprinus carpio l.). | copper is used in treatment mixtures to control fungal diseases in vineyards. its concentrations are relatively high in some aquatic ecosystems, and the main problem observed in this study was the antioxidant stress induced by this heavy metal. copper toxicologic effects in aquatic organisms have prompted the demand for alternative use of low-toxicity molecules in culture treatments. chitosan is a polymer with antifungal property similar to copper and may be an interesting biopesticide. thus, it ... | 2004 | 15386131 |
| loss of filament-forming ability of myosin by non-enzymatic glycosylation and its molecular mechanism. | carp and scallop myosin and their subfragments (s-1 and rod) were reacted with glucose to investigate the effect of non-enzymatic glycosylation on the functionality of myosin. the filament-forming ability of the myosin rod diminished with the progress of non-enzymatic glycosylation and myosin became soluble in 0.1 m nacl. the inhibition of the self-assembly of myosin molecules occurred chemically as a result of the increase in negative charge repulsion among myosin molecules and, further, physic ... | 2004 | 15388325 |
| calcium-binding protein caldendrin and camkii are localized in spinules of the carp retina. | calcium-binding proteins translate the influx of ca(2+) at excitatory synapses into spatiotemporal signals that regulate a variety of processes underlying synaptic plasticity. in the fish retina, the synaptic connectivity between photoreceptors and horizontal cells undergoes a remarkable plasticity, triggered by the ambient light conditions. with increasing light, the synaptic dendrites of horizontal cells form numerous spinules that are dissolved during dark adaptation. the dynamic regulation o ... | 2004 | 15389610 |
| carbonic anhydrase-related protein viii: autoantigen in paraneoplastic cerebellar degeneration. | serum of a patient with paraneoplastic cerebellar degeneration (pcd) and malignant melanoma had a unique reactivity with purkinje cells that was not observed in serum of patients with pcd and other tumors, or with malignant melanoma without paraneoplastic syndromes. the screening of a human cerebellar complementary dna expression library with the patient's serum resulted in the isolation of the ca8 gene. ca8 encodes carbonic anhydrase-related protein (carp) viii, preferentially expressed in purk ... | 2004 | 15389893 |
| first record of contracaecum spp. (nematoda: anisakidae) in fish-eating birds from zimbabwe. | endoparasites of fish-eating birds, phalacrocorax africanus, p. carbo, anhinga melanogaster and ardea cinerea collected from lake chivero near harare, zimbabwe, were investigated. adult contracaecum spp. were found in the gastrointestinal tract (prevalence 100 % in p. africanus, p. carbo and a. melanogaster; 25 % in a. cinerea). parasite intensity was 11-24 (mean 19) in p. africanus, 4-10 (mean 7) in p. carbo, 4-56 (mean 30) in a. melanogaster and 2 (mean 0.5) in a. cinerea. the cormorants fed m ... | 2004 | 15456162 |
| maintained cardiac pumping in anoxic crucian carp. | like most vertebrates, humans die within minutes when deprived of molecular oxygen (anoxia), in part because of cardiac failure. in contrast, some freshwater turtles can survive anoxia for months at low temperatures, but to do so, they drastically suppress cardiac activity and autonomic cardiovascular control. although carassius carassius, the crucian carp, shares this anoxia tolerance, we show that it has a unique ability among vertebrates to retain normal cardiac performance and autonomic card ... | 2004 | 15459381 |
| response of hexokinase enzymes and the insulin system to dietary carbohydrates in the common carp, cyprinus carpio. | the response of the common carp to diets with varying amounts of digestible starch, provided either as pea meal (lp, hp, 30 and 46% peas, respectively) or as cereal (lw, hw, 30 and 46% wheat, respectively), was studied and compared with the response to a carbohydrate-free protein-rich diet (cf). here we focused on the utilisation of dietary carbohydrates by examining the relationship between dietary starch intake, hepatic hexokinase activities, circulating insulin and muscle insulin receptor sys ... | 2011 | 15460162 |
| uptake of sediment-bound bioavailable polychlorobiphenyls by benthivorous carp (cyprinus carpio). | it is unclear whether accumulation of sediment-bound chemicals in benthivorous fish depends on the degree of sequestration in the sediment like it does for invertebrates. here, we report on the potential of slow and fast desorbing sediment-bound polychlorobiphenyl (pcb) fractions for accumulation in carp (cyprinus carpio) in lake enclosures treated with different nutrient doses. routes of pcb uptake were quantitatively evaluated for 15 pcbs (log kow range 5.6-7.8) using model analysis. fast-deso ... | 2004 | 15461156 |
| [contamination source identification study of organochlorines pesticides in 2000 chinese total diet study]. | the third chinese total diet study to estimate dietary intake of contaminants was carried out in 2000. the levels of hch and ddt residues were less significant in most of foodstuffs, but the level of hch residues of aquatic foods in south 2 and in north 1 is respectively 75.99microg/kg and 170.72microg/kg, and the ratio of gamma-hch is 90% of the total hchs residues. to identify the contamination source of gamma-hch, the individual province composite samples and the individual samples of aquatic ... | 2004 | 15461282 |
| myxosporea: a new look at long-known parasites of fish. | with the identification of myxosporea as agents of swimbladder inflammation (sbi) in carp, and proliferative kidney disease (pkd) in salmonids, this group of over 1000 protistan species has been receiving renewed attention. almost all species are parasites offish, and some can cause serious losses in commercial fish farms. yet adequate control measures cannot be planned without clarification of basic features of the parasites' life cycles and modes of transmission. in this article, jiri lom revi ... | 1987 | 15462878 |
| characterization and comparison of fatty acyl delta6 desaturase cdnas from freshwater and marine teleost fish species. | fish are the most important dietary source of the n-3 highly unsaturated fatty acids (hufa), eicosapentaenoic (epa) and docosahexaenoic acid (dha), that have particularly important roles in human nutrition reflecting their roles in critical physiological processes. the objective of the study described here was to clone, functionally characterize and compare expressed fatty acid desaturase genes involved in the production of epa and dha in freshwater and marine teleost fish species. putative fatt ... | 2004 | 15465674 |
| [triploid crucain carp-allotetraploid hybrids (male) x goldfish( hermaphrodite)]. | a new type of triploid crucian carp was generated by mating the males of the allotetraploid hybrids of red crucian carp (carassius auratus red var.) (female) x common carp (cyprinus carpio l.) (male), with the females the goldfish( carassius auratus red var.) which was red in color and possessed the biforked tails. the biological characteristics of one-year old triploid crucian carp including the number and karotype of chromosome, gonadosomatic indices and gonadal structures, appearance and grow ... | 2004 | 15468916 |
| cytoplasmic impact on cross-genus cloned fish derived from transgenic common carp (cyprinus carpio) nuclei and goldfish (carassius auratus) enucleated eggs. | in previous studies of nuclear transplantation, most cloned animals were obtained by intraspecies nuclear transfer and are phenotypically identical to their nuclear donors; furthermore, there was no further report on successful fish cloning since the report of cloned zebrafish. here we report the production of seven cross-genus cloned fish by transferring nuclei from transgenic common carp into enucleated eggs of goldfish. nuclear genomes of the cloned fish were exclusively derived from the nucl ... | 2005 | 15469998 |
| analyses of impact of metal ion contamination on carp (cyprinus carpio l.) gill cell suspensions. | the decline in fish population because of water contamination is problem. as a result of direct exposure in water, it has been readily accepted that the gills are the main site of water contamination and toxicity (e.g., metal ions). in the present study, we investigated metal ion contamination on the functional capacity of carp gill cells with antioxidant interactions in an in vitro study. the extent of cellular membrane damage, lipid peroxidation (lpo) (as tbars levels), and glutathione (gsh) c ... | 2004 | 15475620 |
| use of a complex approach for assessment of metamizole sodium and acetylsalicylic acid toxicity, genotoxicity and cytotoxicity. | a complex approach based on the use of test organisms belonging to different systematic groups (plants, invertebrates and vertebrates), as well as the nucleolar biomarker and the micronucleus test on their cells, was applied to assess the toxicity, cytotoxicity and genotoxicity of two pharmaceutical substances (metamizole sodium and acetylsalicylic acid) applied at ic(50) concentrations for mammalian cells. the compound acetylsalicylic acid was evaluated at a concentration (1.6 x 10(3) mg l(-1)) ... | 2015 | 15478170 |
| three novel carp cxc chemokines are expressed early in ontogeny and at nonimmune sites. | three novel cxc chemokines were identified in common carp (cyprinus carpio l.) through homology cloning. phylogenetic analyses show that one of the three cxc chemokines is an unambiguous orthologue of cxcl14, whereas both others are orthologues of cxcl12, and were named cxcl12a and cxcl12b. percentages of amino acid identity between each of these carp chemokines and their human and mouse orthologues are markedly higher than those reported previously for other carp cxc chemokines, suggestive of i ... | 2004 | 15479238 |
| [karyotypic analysis from two clones of gynogenetic pengze crucian carp (carassius auratus of pengze)]. | pengze crucian carp (carassius auratus of pengze), one of the most popular cultural fishes in china, is an endemic bisexual population with natural gynogenetic reproduction mode. just like silver crucian carp, investigation on the karyotype of pengze crucian carp is important to understanding the diversity and the evolution of the unisexual vertebrates. two distinguishable gynogenetic clones of pengze crucian carp, named clone h and clone l, respectively,were detected recently. in this study, th ... | 2004 | 15481531 |
| detection of koi herpesvirus in common carp, cyprinus carpio l., by loop-mediated isothermal amplification. | loop-mediated isothermal amplification (lamp) is a novel method that amplifies dna with high specificity and rapidity under isothermal conditions. in this study, using the lamp method, a protocol for koi herpes virus (khv) detection in common carp was designed. a set of four primers, two inner and two outer, were designed based on the sequence of the thymidine kinase (tk) gene of khv. time and temperature conditions for detection of khv were optimized for 60 min at 65 degrees c. the detection li ... | 2004 | 15482423 |
| [polymorphism in intron 2 of crucian carp gh i gene]. | high length and nucleotide polymorphisms in intron2 of gh i gene were detected in 162 individuals,which were from seven wild crucian carp colonies, two goldfish colonies and one fangzheng crucian carp colony. using denaturating polyareylamide gel electrophoresis (dpage) and single-strand conformation polymorphism (sscp), seven length variants and 15 haplotypes were identified in these fishes. the length types and haplotypes diversity was 4.32% and 9.26%, respectively. sequence analysis of the 15 ... | 2004 | 15490876 |
| cloning and expression analysis in mature individuals of two chicken type-ii gnrh (cgnrh-ii) genes in common carp (cyprinus carpio). | gonadotropin-releasing hormone (gnrh) is a conservative neurodecapeptide family, which plays a crucial role in regulating the gonad development and in controlling the final sexual maturation in vertebrate. two differing cgnrh-ii cdnas of common carp, namely cgnrh-ii cdna1 and cdna2, were firstly cloned from the brain by rapid amplification of cdna end (race) and reverse transcription- polymerase chain reaction (rt-pcr). the length of cgnrh-ii cdna1 and cdna2 was 622 and 578 base pairs (bp), resp ... | 2004 | 15493476 |
| impact of some chlorinated pesticides on the haematology of the fish cyprinus carpio and puntius ticto. | the effects of pesticides on blood characteristics and histological changes in erythrocytes of the fish species cyprinus carpio and puntius ticto were studied. the fishes were exposed to sub lethal concentrations of different chlorinated pesticides namely aldrin, dieldrin, ddt, bhc and chlordane for 10, 20 and 30 d in continuous flow-through test. the lc50 values were calculated based on acute toxicity tests and the sublethal doses were arrived at for chronic bioassay studies. results showed an ... | 2004 | 15495970 |
| carbonic anhydrases: current state of the art, therapeutic applications and future prospects. | carbonic anhydrases (cas, ec 4.2.1.1) are wide-spread enzymes, present in mammals in at least 14 different isoforms. some of these isozymes are cytosolic (ca i, ca ii, ca iii, ca vii, ca xiii), others are membrane-bound (ca iv, ca ix, ca xii and ca xiv), ca v is mitochondrial and ca vi is secreted in the saliva and milk. three cytosolic acatalytic forms are also known (carp viii, carp x and carp xi). the catalytically active isoforms, which play important physiological and patho-physiological fu ... | 2004 | 15499993 |
| mycobacterium marinum strains can be divided into two distinct types based on genetic diversity and virulence. | mycobacterium marinum causes a systemic tuberculosis-like disease in a large number of poikilothermic animals and is used as a model for mycobacterial pathogenesis. in the present study, we infected zebra fish (danio rerio) with different strains of m. marinum to determine the variation in pathogenicity. depending on the m. marinum isolate, the fish developed an acute or chronic disease. acute disease was characterized by uncontrolled growth of the pathogen and death of all animals within 16 day ... | 2004 | 15501758 |
| fumonisin b1 causes multiple lesions in common carp (cyprinus carpio). | year-1 carp were fed ratios containing 100mg/kg and 10 mg/kg of added fumonisin b1 for 42 days. the experimental and control fish were examined clinically during the experiment and at the end all fish were necropsied and histological changes recorded. blood vessels, liver, exocrine and endocrine pancreas, excretory and haematopoietic kidney, heart and brain were sensitive both to 100 and 10mg/kg of fb1 in the diet and the rodlet cell (rc) frequency was considerably increased in and around damage ... | 2004 | 15503536 |
| [studies on the ploymorphic of sperm of f2 hybrids of red crucian carp x common carp]. | athe ultrastructures of the sperm of f2 hybrids of red crucian carp x common carp were studied by using scanning and transmission electron microscope. the sperm of the f2 hybrids consisted of head, mid-piece and tail. there was no acrosome at the anterior end of the nuclears, whereas there was a vesicle. the results revealed that there existed obviously ploymorphic in the sperm of f2 hybrids. in the water-like semen from males of f2 hybrids, different sizes of the head of the sperm including hap ... | 2004 | 15511069 |
| determination of a bound musk xylene metabolite in carp hemoglobin as a biomarker of exposure by gas chromatography-mass spectrometry using selected ion monitoring. | musk xylene (mx) is widely used as a fragrance ingredient in commercial toiletries. identification and quantitation of a bound 4-amino-mx (amx) metabolite was carried out by gas chromatography-mass spectrometry (gc-ms) with selected ion monitoring (sim). detection of amx occurred after the cysteine adducts in carp hemoglobin (hb), derived from the nitroso metabolite, were released by alkaline hydrolysis. the released amx metabolite was extracted into n-hexane. the extract was preconcentrated by ... | 2004 | 15516317 |
| disparate opinions regarding indications for coronary artery revascularization before elective vascular surgery. | despite consensus guidelines, the optimal strategy for preoperative cardiac risk management among patients scheduled for major noncardiac surgery remains controversial. this study assesses current opinion about the role of preoperative coronary revascularization for patients with coronary artery disease scheduled for elective vascular surgery. thirty-one practicing cardiologists recruited from 4 different regions reviewed case records, imaging tests, and coronary angiograms of 12 patients with c ... | 2004 | 15518605 |
| protection against experimental aeromonas salmonicida infection in carp by oral immunisation with bacterial antigen entrapped liposomes. | liposome-entrapped atypical aeromonas salmonicida antigen was prepared to investigate the potential protective efficacy for a. salmonicida infection. carp (cyprinus carpio) were immunised orally with liposome-entrapped a. salmonicida antigen. after immunisation, significantly higher antigen-specific antibodies were detected in serum, intestinal mucus and bile than non-immunised control group. furthermore, immunised carp were challenged by immersion with 1 x 10(6) cfu ml(-1) of a. salmonicida for ... | 2005 | 15519542 |
| concentrations of a koi herpesvirus (khv) in tissues of experimentally infected cyprinus carpio koi as assessed by real-time taqman pcr. | the koi herpesvirus (khv) is a herpes-like virus now recognized as a worldwide cause of mortality among populations of koi cyprinus carpio koi and common carp cyprinus carpio carpio. temperature is a key factor influencing virus replication both in cell culture and in the tissues of experimentally infected fish. genomic dna sequences were used to optimize a rapid real-time taqman pcr assay to detect and quantify khv dna as found in the tissues of virus-exposed fish. the assay allowed analytical ... | 2004 | 15521316 |
| comparative and evolutionary analysis of the cytochrome b sequences in cyprinids with different ploidy levels derived from crosses. | the mitochondrial cyt b genes in the allotetraploid and triploid crucian carp as well as triploid common carp were isolated and completely sequenced. their dna sequences were compared with those derived from the cyt b genes of the red crucian carp, japanese crucian carp, and common carp with mega 1.0 software. phylogenetic analysis revealed the sister relationships between allotetraploid and diploid red crucian carp, between the triploid crucian carp and diploid japanese crucian carp, and betwee ... | 2004 | 15521428 |
| subchronic oral toxicity of microcystin in common carp (cyprinus carpio l.) exposed to microcystis under laboratory conditions. | the subchronic oral toxicity of microcystin in common carp (cyprinus carpio l.) was investigated in this study. the fish (mean body weight of 322+/-36 g, n=10) were orally exposed to microcystis by feeding with bloom scum at a dose of 50 microg microcystins/kg body weight under laboratory conditions for 28 days. growth assay results showed that microcystin could completely inhibit the growth of carp, but failed to change the fish hepatosomatic index. ultrastructural examination by electron micro ... | 2004 | 15530963 |
| temperature and the expression of myogenic regulatory factors (mrfs) and myosin heavy chain isoforms during embryogenesis in the common carp cyprinus carpio l. | embryos of the common carp, cyprinus carpio l., were reared from fertilization of the eggs to inflation of the swim bladder in the larval stage at 18 and 25 degrees c. crna probes were used to detect transcripts of the myogenic regulatory factors myod, myf-5 and myogenin, and five myosin heavy chain (myhc) isoforms during development. the genes encoding myf-5 and myod were switched on first in the unsegmented mesoderm, followed by myogenin as the somites developed. myf-5 and myod transcripts wer ... | 2004 | 15531645 |
| coping with cold: an integrative, multitissue analysis of the transcriptome of a poikilothermic vertebrate. | how do organisms respond adaptively to environmental stress? although some gene-specific responses have been explored, others remain to be identified, and there is a very poor understanding of the system-wide integration of response, particularly in complex, multitissue animals. here, we adopt a transcript screening approach to explore the mechanisms underpinning a major, whole-body phenotypic transition in a vertebrate animal that naturally experiences extreme environmental stress. carp were ex ... | 2004 | 15550548 |
| [cloning and expression analysis in mature individuals of salmon gonadotropin-releasing hormone (sgnrh) gene in common carp]. | two types of complementary dnas (cdna) encoding the precursor of salmon gonadotropin-releasing hormone (sgnrh, [trp7, leu8] gnrh) are cloned and sequenced from common carp brain using rapid amplification of cdna ends (race). the two cdnas are referred to sgnrh cdna1 and cdna2, and the full-length fragment of cdna1 and cdna2 were 393 and 478 bp, respectively. two sgnrh cdnas contain an open reading frame of 285 bp, which encodes the sgnrh precursor including 94 amino acid residues. the sgnrh prec ... | 2004 | 15552041 |
| stimulation of immunity in indian major carp catla catla with herbal feed ingredients. | catla catla, catla (150 +/- 20 g) were fed a diet containing seed of achyranthes aspera (0.5%) and control diet without a. aspera for four weeks prior to and after ip injection with chicken erythrocytes. fish were sampled for four consecutive weeks after immunization. hemagglutination antibody titers were significantly higher in the test group of fishes compared with the control group. serum globulin levels were significantly (p(t-test) < 0.05) higher in the test group than control group on days ... | 2005 | 15561562 |
| annual evolution of fatty acid profile from muscle lipids of the common carp (cyprinus carpio) in madagascar inland waters. | annual evolution of muscle lipids fatty acid (fa) from common carp (cyprinus carpio) has been determined in 2001 through monthly samplings in the reserve pond of sisaony (sis series) and itasy lake (ita series) of the madagascar highlands. total lipids from muscle were extracted and quantified according to the bligh and dyer method. fa identification was performed by gc-ms of fa methyl esters and fa pyrrolidides and led to the identification of 41 fa; routine analyses of fa were made by capillar ... | 2004 | 15563217 |
| the application of image cytometry to viability assessment in dual fluorescence-stained fish spermatozoa. | the viability of spermatozoa has been assessed using sybr 14 staining for dna of living cells and propidium iodide staining for dna of degenerate cells. this dual staining was performed on four fish species (siberian sturgeon, acipenser baerii; common carp, cyprinus carpio; tench, tinca tinca and wels, silurus glanis) and the proportions of live and dead spermatozoa were assessed by epifluorescence microscopy and image cytometry. ten phase contrast and epifluorescent images were recorded per sam ... | 2004 | 15566965 |
| complete nucleotide sequence of the hirame rhabdovirus, a pathogen of marine fish. | reverse transcription-polymerase chain reaction (rt-pcr) derived clones were constructed for the hirame rhabdovirus (hirrv) ca-9703 strain from korea, and the dna was sequenced. the 3'-end of genomic rna was cloned by poly(a)-tailing of the genomic rna before reverse transcription, and the 5'-end of the genome was cloned by poly(g)- or poly(c)-tailing of the first strand, followed by pcr. the remainder of the genomic dna was cloned by reverse transcription-polymerase chain reaction using primers ... | 2005 | 15567027 |
| genetic variation analysis within and among six varieties of common carp (cyprinus carpio l.) in china using microsatellite markers. | five microsatellites were used to study the genetic diversity and genetic structure of one wild and five domestic varieties of common carp in china (the yangtze river wild common carp, xingguo red carp, purse red carp, qingtian carp, russian scattered scaled mirror carp and japanese decorative carp). all loci in this study showed marked polymorphism with the number of alleles from 4 to 13. domestic varieties (except xingguo red carp) showed less genetic diversity than the yangtze river wild comm ... | 2004 | 15575506 |
| sequence and expression of a constitutive, facilitated glucose transporter (glut1) in atlantic cod gadus morhua. | a putative glucose transporter, glut1, is reported for atlantic cod gadus morhua. a combination of rt-pcr, rlm-race and genome walking were used to articulate a 4560 bp cdna (genbank accession number ay526497). it contains a 149 bp 5' utr, a 1470 bp open reading frame and a 2941 bp 3' utr. at the nucleotide level, the cod glut1 orf shares 78.2% sequence identity to human glut1 and the deduced amino acid sequence clusters with glut1s from rainbow trout and carp. glut1 transcript is highly express ... | 2004 | 15579563 |
| a novel serine protease complexed with alpha2-macroglobulin from skeletal muscle of lizard fish (saurida undosquamis). | a novel fish muscle serine protease named muscle soluble serine protease (mssp) was purified from the soluble fraction of lizard fish (saurida undosquamis: synodontidae) muscle by ammonium sulfate fractionation followed by four steps of column chromatographies. in native-page, the purified enzyme appeared as a single band with an estimated mol. mass of approximately 380 kda by gel filtration. in sds-page under reducing conditions, the purified enzyme migrated as two protein bands at 110 and 100 ... | 2004 | 15581796 |
| ultrastructural identification of a herpes-like virus infection in common carp cyprinus carpio in korea. | we report on a herpes-like virus, which was found to be associated with mass mortality of common carp cyprinus carpio for the first time in korea in 1998. the external signs of infection in moribund fish were darkened coloration and severe branchial necrosis in the gill. transmission electron microscopy revealed the presence of herpes-like viruses in spleen tissue. infected spleen cells showed hypertrophied nuclei and degeneration. numerous nucleocapsids of about 82 nm in diameter were found wit ... | 2004 | 15584424 |
| improvement of transfection efficiency of epithelioma papulosum cyprini carp cells by modification of cell cycle and use of an optimal promoter. | several methods to improve transfection of epithelioma papulosum cyprini (epc) carp cells have been tested and are reported here. by modifying the cell cycle state of epc cell monolayers and selecting the best promoter for the plasmid to be transfected, we increased transfection efficiency from 12.8% to 55.1% and decreased the coefficient of variation among different experiments from 54.1% to 11.8%. thus 2- to 3-fold higher transfection efficiencies were obtained when the epc monolayers were tre ... | 2004 | 15592944 |
| molecular phylogeny of the kidney-parasitic sphaerospora renicola from common carp (cyprinus carpio) and sphaerospora sp. from goldfish (carassius auratus auratus). | the genetic relatedness of two kidney-parasitic sphaerospora species was studied. although s. renicola, the causative agent of swimbladder inflammation of common carp fingerlings (cyprinus carpio), and sphaerospora sp. originating from goldfish (carassius auratus auratus) were indistinguishable on the basis of spore morphology, they were found to be genetically different as their 18s rdna sequences shared only 71.9% identical nucleotides. in the phylogenetic trees, sphaerospora sp. from goldfish ... | 2004 | 15595280 |
| aspartic acid scanning mutation analysis of a goldfish growth hormone-releasing hormone (ghrh) receptor specific to the ghrhsalmon-like peptide. | growth hormone-releasing hormone (ghrh) plays a pivotal role in the regulation of growth. the study of goldfish ghrh and its receptor is of particular interest as it is so far the only animal model in which two forms of ghrh-like (catfish-like and salmon-like) peptides coexist, and these peptides share only 30-40% of amino acid sequence identities with their mammalian counterparts. for these reasons, we have previously characterized a goldfish ghrh receptor, which is specific for a synthetic car ... | 2005 | 15596070 |
| reproductive responses of common carp (cyprinus carpio) exposed in cages to influent of the las vegas wash in lake mead, nevada, from late winter to early spring. | the las vegas wash (lw) delivers tertiary-treated municipal wastewater effluent, nonpotable shallow groundwater seepage, and runoff from the urbanized las vegas valley to las vegas bay (lx) of lake mead. to investigate the potential for contaminants in lw influent to produce effects indicative of endocrine disruption in vivo, adult male and female common carp (cyprinus carpio) were exposed in cages for 42-48 d at four sites in lake mead: lw, lx, and two reference locations in the lake. end point ... | 2004 | 15597896 |
| the strengths of in vivo magnetic resonance imaging (mri) to study environmental adaptational physiology in fish. | adaptational physiology studies how animals cope with their environment, even if this environment is subject to permanent fluctuations such as tidal or seasonal variations. aquatic organisms are generally more prone to be exposed to osmotic, hypoxic and temperature challenges than terrestrial animals. some of these challenges are more restraining in an aquatic environment. to date, very few studies have used in vivo magnetic resonance imaging (mri) to uncover the physiological mechanisms that re ... | 2004 | 15614515 |
| brain responses to ambient temperature fluctuations in fish: reduction of blood volume and initiation of a whole-body stress response. | spatial and temporal ambient temperature variations directly influence cellular biochemistry and thus the physiology of ectotherms. however, many aquatic ectothermic species maintain coordinated sensorimotor function during large acute body-temperature changes, which points to a compensatory mechanism within the neural system. here we used high-resolution functional magnetic resonance imaging to study brain responses to a drop of 10 degrees c of ambient water temperature in common carp. we obser ... | 2005 | 15615828 |
| pentachlorophenol reduces b lymphocyte function through proinflammatory cytokines in carassius auratus. | cytokines and igm levels are important parameters in the acquired immunity of fish. in the present study, the effect of pentachlorophenol (pcp) on the expression of proinflammatory cytokines, tnf-alpha (tumor necrosis factor-alpha) and il-1beta (interleukin 1) mrna levels of crucian carp, were determined by semi-quantitative reverse transcriptase-polymerase chain reaction (rt-pcr) method. to put the deduction of pcp's immunotoxicity on b cell function, b cells secretion of igm under exposure of ... | 2005 | 15621336 |
| carp liver dnase--isolation, further characterization and interaction with endogenous actin. | deoxyribonuclease i (dnase i)-like enzyme from the liver of the carp (cyprinus carpio) was purified to homogeneity and further characterized. ion exchange chromatography on deae-cellulose, molecular filtration on sephacryl s-300 and con a-sepharose affinity chromatography were applied for enzyme isolation. carp liver dnase, similarly to dnase i from bovine pancreas, was found to be an endonuclease that hydrolyses linear dna from salmon sperm as well as circular dna forms--plasmid and cosmid. the ... | 2005 | 15621519 |
| carp, a cardiac ankyrin repeat protein, is up-regulated during wound healing and induces angiogenesis in experimental granulation tissue. | cardiac ankyrin repeat protein (carp) was identified by subtractive hybridization as one of a group of genes that are rapidly modulated by acute wounding of mouse skin. quantitative rt-pcr showed that carp was strongly induced during the first day after wounding (157.1-fold), and the high level persisted for up to 14 days. immunohistochemistry and in situ hybridization revealed that carp was expressed in skeletal muscle, vessel wall, hair follicle, inflammatory cells, and epidermis in the wound ... | 2005 | 15632022 |
| dna aggregating protein from carp (cyprinus capio) eggs. | hemagglutinating activity for glutaraldehyde-fixed, trypsinized human and rabbit erythrocytes was obtained from carp (ciprinus capio) egg extract. this activity was inhibited by sulfated glycans such as heparin and fucoidan, and salmon testis dna (stdna), but not by mono- and oligosaccharides. the active fraction from hydroxyapatite column, ha400, caused aggregation of stdna at a concentration of 125 microg/ml. ha400-induced aggregation was inhibited by heparin and fucoidan at the concentrations ... | 2005 | 15635155 |
| melatonin accelerates maturation inducing hormone (mih): induced oocyte maturation in carps. | the present communication is an attempt to demonstrate the influence of melatonin on the action of maturation inducing hormone (mih) on the maturation of oocytes in carps. the oocytes from gravid female major carp labeo rohita were isolated and incubated separately in medium 199 containing (a) only mih (1 microg/ml), (b) only melatonin (at concentrations of 50, 100 or 500 pg/ml), and (c) both melatonin and mih, but at different time intervals. in the latter group, melatonin was added to the incu ... | 2004 | 15639142 |
| plasma alpha-msh and acetylated beta-endorphin levels following stress vary according to crh sensitivity of the pituitary melanotropes in common carp, cyprinus carpio. | pituitary melanotropes release alpha-melanocyte-stimulating hormone (alpha-msh) and acetylated beta-endorphin (nac beta-end) during stress responses. however, effects of stressors on plasma concentrations of these hormones are highly inconsistent among fish species. here, we show that also within a species, the common carp (cyprinus carpio), fish sometimes respond with elevated alpha-msh and nac beta-end plasma levels, and at other times not. the origin of this variable response was investigated ... | 2005 | 15639149 |
| [the analysis of genetic variability of mtdna 12s rrna genes in the allotetraploid, triploid crucian carp and their parents]. | the complete 12s rrna genes from the five f11 allotetraploids, two diploid gynogens, two triploid crucian carps, one red crucian carp, one xiangjiang common carp, and one japanese crucian carp were successfully cloned and sequenced. sequence compositions and variations were analyzed with mega 1.0 software. five f11 allotetraploids shared two haplotypes, and two diploid gynogens, two triploid crucian carps, one red crucian carp, one xiangjiang common carp, one japanese crucian carp shared one hap ... | 2004 | 15640120 |
| role of ca2+ store in ampa-triggered ca2+ dynamics in retinal horizontal cells. | fura-2 fluorescent calcium imaging was applied to measure [ca(2+)](i) in freshly dissociated horizontal cells of carp retina, and a model containing endoplasmic reticulum (er) membrane processes and plasma membrane processes was constructed for quantitative analyses of the ampa-triggered calcium dynamics. a transient increase followed by a sustained steady level of [ca(2+)](i) was observed when 100 microm ampa was applied, while the initial transient increase of [ca(2+)](i) was suppressed by exo ... | 2004 | 15640746 |
| clinical trials update from the american heart association meeting: acorn-csd, primary care trial of chronic disease management, peace, create, shield, a-heft, gemini, vitamin e meta-analysis, escape, carp, and scd-heft cost-effectiveness study. | this article provides information and a commentary on landmark trials presented at the american heart association meeting held in november 2004, relevant to the pathophysiology, prevention, and treatment of heart failure. an open trial of the acorn cardiac support device (csd) showed encouraging preliminary results in patients with severe heart failure. the peace (prevention of events with angiotensin-converting enzyme inhibition) study supports data from previous studies showing that ace inhibi ... | 2005 | 15642544 |
| impact of pcb mixture (aroclor 1254) and tbt and a mixture of both on swimming behavior, body growth and enzymatic biotransformation activities (gst) of young carp (cyprinus carpio). | the sensitivity of carp to chronical stress due to single and combined mixtures (each 50%) of tbt and pcb was evaluated under laboratory conditions. and concluded, mixed exposures were partially more or less toxic than single ones. xenobiotic stress led to both decreased mean daily swimming speed and an increased mean swimming speed during darkness. also a decreased preferred swimming depth (nearer to the surface) during nighttime was observed. we found approximately synergistic effects of tbt a ... | 2005 | 15642631 |
| effects of growth hormone on leucine absorption, intestinal morphology, and ultrastructure of the goldish intestine. | the mechanisms whereby exogenous growth hormone modulates intestinal structure and function in fish were investigated. goldfish (carassius auratus) were fed commercial flake diet sprayed with recombinant carp growth hormone (cgh) daily for 1 month. control animals received food sprayed with the vehicle. after 1 month of daily feedings, body mass and length were determined, and animals were sacrificed to study intestinal characteristics. sections of foregut were removed after determination of tot ... | 2004 | 15644934 |
| [experimental studies on the central analgesic effect of the non-steroidal anti-inflammatory drug carprofen in a sheep model -- preliminary results]. | to evaluate the effect of the non-steroidal anti-inflammtory drug carprofen on the lumbar cerebrospinal fluid (csf) concentration of noradrenaline (na) and serotonin (5-ht) in non-stimulated (part i) and surgically stimulated (part ii) sheep. | 2005 | 15645384 |
| identification of estrogenic compounds in wastewater effluent. | in order to identify the dominant contributors to estrogenic activity in environmental waters, a comprehensive fractionation method using silica gel column chromatography, combined with recombinant yeast assay for detecting estrogenic activity and with gas chromatography-mass spectrometry for quantifying endocrine disruptors and natural estrogens, was developed. the method was applied to the municipal sewage treatment plant (stp) secondary effluent discharged to the tamagawa river in tokyo, japa ... | 2004 | 15648753 |
| the cestode atractolytocestus huronensis (caryophyllidea) continues to spread in europe: new data on the helminth parasite of the common carp. | the caryophyllidean tapeworm atractolytocestus huronensis anthony, 1958, originally described from the common carp cyprinus carpio carpio l. from north america, has recently been introduced into europe (uk, hungary). in the present study, the dispersion of the tapeworm in other countries of central europe (slovakia and the czech republic) is described, including its first observation in the common carp of the tisa river, southeastern slovakia. because of the transboundary location of the river, ... | 2004 | 15648838 |
| effect of hexavalent carcinogenic chromium on carp cyprinus carpio immune cells. | chromium is widely used in industrial processes, and is released into aquatic environments by electroplating, tannery and textile industries. fishes in natural waters or in aquaculture facilities supplied with these waters are exposed to chromium waste and are presumed to be affected by deposits. herein, we examine the effect of hexavalent chromium on carp cyprinus carpio derived immune cells. in vitro exposure of carp leukocytes to hexavalent chromium induced cytotoxicity, decreased mitogen-ind ... | 2004 | 15648842 |
| genetic variability and relationships in mitochondrial dna co ii gene sequence of red common carps in china. | nucleotide sequence of cytochrome coxidase subunit ii (co ii) of mitochondrial dna ( mtdna) was analyzed from four populations of the red common carp (c. c. var. singuonensis, c. c. var. wananensis, c. c. var. wuyuanensis and c. c. var. color) as well as one population of wild common carp in china to detect their genetic variability and phylogenetic relationship. nineteen nucleotide variable positions were detected in analyzed 607 bp length sequence and 15 haplotypes were defined among five popu ... | 2004 | 15651674 |
| effect of ultrasound on nucleated erythrocytes. | in this experimental study, carp erythrocytes were used as nucleated cell models to test the hypothesis that ultrasound (us) exposure can cause the change in plasma membranes and hemoglobin. to identify target cell damage, we studied hemolysis, osmotic fragility, lipid peroxidation of red blood cells and oxidation of hemoglobin in the erythrocytes. the erythrocytes were exposed to 1 mhz continuous-wave us at the intensities of 0.61 to 2.44 w/cm2 for 5 min. these results showed that us sonication ... | 2005 | 15653240 |
| noise emission during the first powerboat race in an alpine lake and potential impact on fish communities. | in order to assess the effects of high-speed boating on fish communities, noise levels were measured during the first class 1 powerboat race on the austrian lake traunsee. the noise spectra were compared to natural ambient noise and hearing abilities of four native fish species. sound pressure levels (spls) were significantly elevated during the training heats and the race compared with natural levels, reaching up to 128 db re 1 micropa (instantaneous spl) at a distance of 300 m to the powerboat ... | 2004 | 15658729 |
| opisthorchiasis from imported raw fish. | liver fluke infection caused by opisthorchiidae is a major public health problem in many parts of the far east, southeast asia, and eastern europe. however, with the growing volume of international travel and population migration, the infection is increasingly diagnosed in countries where the disease is not endemic, particularly in north america. we report an outbreak of acute opisthorchiasis in a family that was infected in a non-disease-endemic area after eating raw carp illegally imported fro ... | 2004 | 15663848 |
| elevated dna-protein cross-links in red blood cells of german carp, cyprinus carpio, from the lower passaic river, new jersey, usa. | 2004 | 15669735 | |
| toxicity and bioaccumulation of chlorpyrifos in indian carp catla catla (hamilton), labeo rohita (hamilton), and cirrhinus mrigala (hamilton). | 2004 | 15669740 | |
| using mitochondrial nucleotide sequences to investigate diversity and genealogical relationships within common carp (cyprinus carpio l.). | direct sequencing of mitochondrial dna (mtdna) d-loop (745 bp) and mtatpase6/mtatpase8 (857 bp) regions was used to investigate genetic variation within common carp and develop a global genealogy of common carp strains. the d-loop region was more variable than the mtatpase6/mtatpase8 region, but given the wide distribution of carp the overall levels of sequence divergence were low. levels of haplotype diversity varied widely among countries with chinese, indonesian and vietnamese carp showing th ... | 2005 | 15670127 |
| inhibition of whole body ca2+ uptake in fresh water teleosts, channa punctatus and cyprinus carpio in response to salmon calcitonin. | in many fish, ultimobranchial-derived calcitonin (ct) has been shown to be a potent hypocalcemic regulator. but an equal number of studies failed to show any correlation between ct and plasma calcium levels. again, in fish, where ct has been shown to function as a hypocalcemic hormone, the way this is brought about is not well known. while the regulatory role of many hormones e.g., stanniocalcin, pituitary-derived prolactin and cortisol on gill calcium (ca2+) transport (gcat) has been well estab ... | 2004 | 15673109 |
| avoidance behavior and brain monoamines in fish. | the crucian carp performs a typical avoidance behavior when exposed to olfactory cues from injured skin of conspecifics. they swim rapidly to the bottom and hide in available material. this work examines the effects of skin extract exposure and availability of hiding material on this behavior, and concomitant changes in brain monoaminergic activity in crucian carp. individual fish were exposed to skin extract in aquaria with or without hiding material. exposure to skin extract resulted in the ex ... | 2005 | 15680947 |
| a separation-free amperometric immunosensor for vitellogenin based on screen-printed carbon arrays modified with a conductive polymer. | a disposable amperometric immunosensor was studied for the rapid detection of carp (carassius auratus) vitellogenin (vtg). the sensor was fabricated based on screen-printed carbon arrays (spcas) containing eight carbon working and an integrated carbon counter electrodes. to construct the sensor, a conducting polymer (poly-terthiophene carboxylic acid) was electropolymerized on the surface of working electrodes and the polymer-coated spcas was characterized by sem. horseradish peroxidase (hrp) an ... | 2005 | 15681194 |
| description of an as yet unclassified dna virus from diseased cyprinus carpio species. | numerous deaths of koi and common carp (cyprinus carpio) were observed on many farms throughout israel, resulting in severe financial losses. the lethal viral disease observed is highly contagious and extremely virulent, but morbidity and mortality are restricted to koi and common carp populations. diseased fish exhibit fatigue and gasping movements in shallow water. infected fish had interstitial nephritis and gill necrosis as well as petechial hemorrhages in the liver and other symptoms that w ... | 2005 | 15681400 |
| responses of molecular indicators of exposure in mesocosms: common carp (cyprinus carpio) exposed to the herbicides alachlor and atrazine. | common carp (cyprinus carpio) were treated in aquatic mesocosms with a single pulse of the herbicides atrazine or alachlor to study the bioavailability and biological activity of these herbicides using molecular indicators: liver vitellogenin gene expression in male fish for estrogenic activity, liver cytochrome p4501a1 gene expression, and dna damage in blood cells using the single-cell gel electrophoresis method. both alachlor and atrazine showed dose-related increases in dna strand breaks at ... | 2005 | 15683183 |
| partial oxidative-stress perturbs membrane permeability and fluidity of fish nucleated red blood cells. | we investigated the influence of partial oxidative stress on permeability and fluidity of nucleated fish red blood cells for simulating nucleated somatic cells. peroxide value indicating lipid hydroperoxide level in nucleated red blood cells of common carp (cyprinus carpio) increased with increasing body size. we detected that oxidation of nucleated red blood cells led to the degraded pufa compositions and accelerated the permeability of calcein and atp in the nucleated red blood cells restricte ... | 2004 | 15683836 |
| molecular cloning of leukocyte cell-derived chemotaxin 2 in rainbow trout. | in humans, leukocyte cell-derived chemotaxin 2 (lect2) is a 16kda chemotactic protein that consists of 133 amino acids and three intramolecular disulphide bonds. although it was originally demonstrated to have a chemotactic function in vitro, recent data sustain a further multifunctional role of lect2 that extends from cell growth, differentiation, damage/repair process and carcinogenesis to autoimmune diseases. the in vivo function of lect2 protein still remains obscure. in order to study the p ... | 2005 | 15683915 |
| comparative immunohistochemical study of carassius rfamide localization in teleost guts in different salinity habitats. | carassius rfamide (c-rfa) is a peptide, isolated originally from the brain of japanese crucian carp and sharing homologies with mammalian prolactin-releasing peptides. from the physiological aspect, it is known that c-rfa has contraction-promoting action on fish intestines, but its localization in peripheral tissues is unknown. we observed the localization of c-rfa in teleost guts using an immunohistochemical technique. c-rfa-like immunoreactive (irc-rfa) sites were observed in not only the smoo ... | 2005 | 15684584 |
| development and evaluation of an experimental model of cutaneous columnaris disease in koi cyprinus carpio. | a reproducible, experimental model of columnaris disease was developed to study the pathogenesis of cutaneous disease associated with flavobacterium columnare infection in koi (cyprinus carpio). in experimental infections, lesions were usually restricted to skin and fins; gill necrosis was not a consistent finding. cytologic and histopathologic examinations provided a presumptive diagnosis of columnaris disease. specific detection of f. columnare was done using the polymerase chain reaction and ... | 2005 | 15690950 |
| whole-blood and plasma cholinesterase levels in normal koi (cyprinus carpio). | organophosphate and carbamate insecticides produce their toxic effects by binding with cholinesterase enzymes, resulting in the inhibition of acetylcholine catabolism. excessive acetylcholine levels cause excessive synaptic neurotransmitter activity. in most animal species, whole blood is the preferred sample for evaluation of cholinesterase activity. previous reports of cholinesterase activity in carp have involved sampling of serum, plasma, brain, liver, muscle, and heart. whole-blood samples ... | 2005 | 15690957 |
| catalytic and structural characteristics of carp hepatopancreas d-amino acid oxidase expressed in escherichia coli. | d-amino acid oxidase of carp (cyprinus carpio) hepatopancreas was overexpressed in escherichia coli cells and purified to homogeneity for the first time in animal tissues other than pig kidney. the purified preparation had a specific activity of 293 units mg(-1) protein toward d-alanine as a substrate. it showed the highest activity toward d-alanine with a low km of 0.23 mm and a high kcat of 190 s(-1) compared to 10 s(-1) of the pig kidney enzyme. nonpolar and polar uncharged d-amino acids were ... | 2005 | 15694590 |