Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| genotypic diversity of anaerobic isolates from bloodstream infections. | accurate species determination for anaerobes from blood culture bottles has become increasingly important with the reemergence of anaerobic bacteremia and prevalence of multiple-drug-resistant microorganisms. our knowledge of the taxonomical diversity of anaerobes that cause bloodstream infections is extremely limited, because identification historically has relied on conventional methods. over a 5-year period, we profiled anaerobic bacteremia at a large tertiary care hospital with 16s rrna gene ... | 2008 | 18322067 |
| prevalence of periodontal pathogens in subgingival lesions, atherosclerotic plaques and healthy blood vessels: a preliminary study. | previous studies have reported different periodontal bacteria in atherosclerotic lesions, but their involvement in plaque formation remains unclear. the aim of the present study was to investigate the presence of 20 periodontal bacteria in atherosclerotic samples and healthy blood vessels (used as controls) and to clarify their relationship in regard to clinical and bacteriological periodontal status. | 2008 | 18326058 |
| thoracic vertebral actinomycosis: actinomyces israelii and fusobacterium nucleatum. | actinomyces spp. are considered rare pathogens in today's medicine, especially with thoracic vertebral involvement. classic actinomycosis (50%) presents as an oral-cervicofacial ("lumpy jaw") infection. this report describes a case of spinal cord compression caused by actinomyces israelii with the coisolation of fusobacterium nucleatum. there are limited numbers of similar cases. | 2008 | 18337385 |
| antimicrobial activity of sodium hypochlorite and chlorhexidine by two different tests. | the aim of this study was to evaluate the antimicrobial capacity of sodium hypochlorite (1% and 5%) and chlorhexidine (0.12%, 0.5% and 1%) with or without the addition of organic material (bovine serum albumin, bsa) against some bacterial samples (staphylococcus aureus, enterococcus faecalis, escherichia coli, porphyromonas gingivalis and fusobacterium nucleatum) using two activity tests (contact and diffusion agar tests). in the contact test (first model), bacterial samples were kept in contact ... | 2008 | 18352899 |
| pyogenic liver abscess related to dental disease in an immunocompetent host. | a 59-year-old man with poor oral hygiene presented to our hospital because of fever and chills. abdominal ultrasonography and enhanced computed tomography (ct) revealed a liver abscess. the patient had no history of immunodeficiency and we confirmed the patient had no immunologic abnormalities. blood culture revealed fusobacterium nucleatum, a bacterium commonly found in the oral cavity. even if a patient is immunocompetent, poor oral hygiene might be an independent risk factor for a pyogenic li ... | 2008 | 18379160 |
| [detection of periodontal pathogens in coronary atherosclerotic plaques]. | to explore the possible correlation between coronary heart disease and periodontitis. | 2008 | 18380965 |
| bacterial profiles of root caries in elderly patients. | culture-based studies have shown that streptococcus mutans and lactobacilli are associated with root caries (rc). the purpose of the present study was to assess the bacterial diversity of rc in elderly patients by use of culture-independent molecular techniques and to determine the associations of specific bacterial species or bacterial communities with healthy and carious roots. plaque was collected from root surfaces of 10 control subjects with no rc and from 11 subjects with rc. the bacterial ... | 2008 | 18385433 |
| role of porphyromonas gingivalis serb in gingival epithelial cell cytoskeletal remodeling and cytokine production. | the serb protein of porphyromonas gingivalis is a had family serine phosphatase that plays a critical role in entry and survival of the organism in gingival epithelial cells. serb is secreted by p. gingivalis upon contact with epithelial cells. here it is shown by microarray analysis that serb impacts the transcriptional profile of gingival epithelial cells, with pathways involving the actin cytoskeleton and cytokine production among those significantly overpopulated with differentially regulate ... | 2008 | 18391005 |
| the lower genital tract microbiota in relation to cytokine-, slpi- and endotoxin levels: application of checkerboard dna-dna hybridization (cdh). | in the present study the lower genital tract microbiota in asymptomatic fertile women (n=34) was identified and quantified by culturing vaginal secretions. also, vaginal and cervical samples were analyzed by a semiquantitative checkerboard dna-dna hybridization technique (cdh) based on genomic probes prepared from 13 bacterial species (bacteroides ureolyticus, escherichia coli, fusobacterium nucleatum, gardnerella vaginalis, mobiluncus curtisii ss curtisii, prevotella bivia, prevotella disiens, ... | 2008 | 18397461 |
| bleeding on probing differentially relates to bacterial profiles: the oral infections and vascular disease epidemiology study. | various bacterial species are differentially prevalent in periodontal health, gingivitis or periodontitis. we tested the independent associations between three bacterial groupings and gingival inflammation in an epidemiological study. | 2008 | 18400025 |
| [microbiological and biochemical characteristics of inflammatory tissues in the periodontium]. | to investigate bacterial populations in subgingival and supragingival plaque samples of patients with inflammatory periodontal diseases and activities of the lysosomal enzymes--lysozyme, alkaline phosphatase, and beta-glucuronidase--in peripheral venous blood, in gingival crevicular fluid, and mixed nonstimulated saliva. | 2008 | 18413987 |
| microbiological evaluation of primary endodontic infections in teeth with and without sinus tract. | to examine the microbiological status of primary endodontic infections in teeth with and without a sinus tract. | 2008 | 18422583 |
| serum antibodies to porphyromonas gingivalis chaperone htpg predict health in periodontitis susceptible patients. | chaperones are ubiquitous conserved proteins critical in stabilization of new proteins, repair/removal of defective proteins and immunodominant antigens in innate and adaptive immunity. periodontal disease is a chronic inflammatory infection associated with infection by porphyromonas gingivalis that culminates in the destruction of the supporting structures of the teeth. we previously reported studies of serum antibodies reactive with the human chaperone hsp90 in gingivitis, a reversible form of ... | 2008 | 18431474 |
| a microbiological profile of symptomatic teeth with primary endodontic infections. | the aim of this study was to evaluate the composition of the microbiota of primary endodontic infections associated with symptomatic teeth. samples were collected by means of a #15 h-type file and 2 sterile paper points from 60 symptomatic (n = 30) or asymptomatic (n = 30) single-rooted teeth with necrotic pulp. the presence of 40 bacterial species was determined by the checkerboard dna-dna hybridization method. the species found in higher counts (x10(5)) in symptomatic cases were fusobacterium ... | 2008 | 18436031 |
| impact of growth conditions on susceptibility of five microbial species to alkaline stress. | the effects of different growth conditions on the susceptibility of five taxa to alkaline stress were investigated. enterococcus faecalis atcc 29212, streptococcus sobrinus omz 176, candida albicans atcc 90028, actinomyces naeslundii atcc 12104, and fusobacterium nucleatum atcc 10953 were grown as planktonic cells, allowed to adhere to dentin for 24 hours, grown as monospecies or multispecies biofilms on dentin under anaerobic conditions with a serum-enriched nutrient supply at 37 degrees c for ... | 2008 | 18436038 |
| a rare presentation of ventriculitis and brain abscess caused by fusobacterium nucleatum. | anaerobic ventriculitis is rare, and usually seen in patients with predisposing factors such as otitis media, mastoiditis, sinusitis or recent neurosurgery. we report what we believe to be the first case of ventriculitis and brain abscess due to fusobacterium nucleatum infection in a man with no significant predisposing factors. he was successfully treated with antibiotic therapy. | 2008 | 18436604 |
| [prediction on the efficacy of fluoroquinolones for anaerobic infections using monte carlo simulation]. | various analysis based on pk-pd (pharmacokinetics-pharmacodynamics) theory were performed for prediction on the efficacy of antimicrobial agents for infectious diseases. however, there have been few reports when causative organisms would be anaerobic bacteria. although the antimicrobial activity of fluoroquinolones against anaerobes has been weak, gatifloxacin (gflx), moxifloxacin (mflx), and garenoxacin (grnx) are considered as anti-anaerobic quinolones in recent days. therefore, we investigate ... | 2007 | 18447205 |
| effects of age and oral disease on systemic inflammatory and immune parameters in nonhuman primates. | this report evaluated systemic inflammatory and immune biomarkers in a cohort of macaca mulatta (rhesus monkeys) maintained as a large family social unit, including an age range from <1 year to >24 years. we hypothesized that the systemic host responses would be affected by the age, gender, and clinical oral presentation of the population, each contributing to inflammatory and immune responses that would reflect chronic oral infections. the results demonstrated that the prevalence and severity o ... | 2008 | 18448617 |
| ability of oral bacteria to induce tissue-destructive molecules from human neutrophils. | the induction of tissue-destructive molecules from neutrophils by periodontopathic bacteria has been suggested as one of the mechanisms of periodontal destruction. the aim of this study was to determine whether the ability to stimulate neutrophils is an authentic characteristic of periodontopathic bacteria. | 2008 | 18449962 |
| photodynamic therapy as adjunct to non-surgical periodontal treatment in patients on periodontal maintenance: a randomized controlled clinical trial. | recent preclinical and clinical data have suggested the potential benefit of photodynamic therapy (pdt) in the treatment of periodontitis. however, currently, there are very limited data from controlled clinical trials evaluating the effect of pdt in the treatment of periodontitis. the aim of the present study was to evaluate the clinical and microbiological effects of the adjunctive use of pdt in non-surgical periodontal treatment in patients receiving supportive periodontal therapy. twenty-fou ... | 2009 | 18465191 |
| candida dubliniensis and candida albicans display surface variations consistent with observed intergeneric coaggregation. | adherence of yeasts to other microorganisms and epithelial cell surfaces is important in their colonization. comparative studies based on the coaggregation of candida dubliniensis versus candida albicans with fusobacterium nucleatum and other oral bacteria suggested differences in the surfaces of these yeasts. transmission electron microscopy was used to test the hypothesis that there are morphologic variations in the cell surface of these two species. c. dubliniensis type strain cd36 and c. alb ... | 1999 | 18473545 |
| [alterations of salivary flow rate and oral main pathogens in miniature pig with bilateral parotid gland atrophy]. | to observe the effect of bilateral parotid gland atrophy on the whole saliva flow rate and the growth of main oral pathogens in different sites of oral cavity. | 2007 | 18476559 |
| quercetin in a lotus leaves extract may be responsible for antibacterial activity. | in the course of a search for chemotherapeutic agents inhibiting suspected peridontitis bacteria, extracted and purified substances from lotus leaf were identified by antimicrobial activity tests with use of the broth micro-dilution methods on 96-microwell plate. the antimicrobial activity of extracts was tested against five microorganisms, namely: actinobacillus actinomycetemcomitans y4, actinomyces viscosus 19246, porphyromonas gingivalis 33277, fusobacterium nucleatum 25586, and actinomyces n ... | 2008 | 18481022 |
| photodynamic treatment of endodontic polymicrobial infection in vitro. | we investigated the photodynamic effects of methylene blue on multispecies root canal biofilms comprising actinomyces israelii, fusobacterium nucleatum subspecies nucleatum, porphyromonas gingivalis, and prevotella intermedia in experimentally infected root canals of extracted human teeth in vitro. the 4 test microorganisms were detected in root canals by using dna probes. scanning electron microscopy showed the presence of biofilms in root canals before therapy. root canal systems were incubate ... | 2008 | 18498901 |
| the anti-endotoxic effects of the ksl-w decapeptide on escherichia coli o55:b5 and various oral lipopolysaccharides. | host responses following the recognition of bacterial lipopolysaccharide can range from acute inflammation to septic shock. the aim of this study was to evaluate the ability of the ksl-w decapeptide to bind to and block the endotoxic effects of lipopolysaccharide. | 2008 | 18503516 |
| [periodontitis and cardiovascular disease]. | the prevalence of periodontitis and cardiovascular disease (cvd) is high. a mixed infectious biofilm etiology of periodontitis is known but not fully established in cvd. cofactors; smoking habits, stress, ethnicity, genetics, socioeconomics and age contribute to both diseases. the objectives of this report are to summarize factors in regards to cvd and periodontitis that are clinically relevant. the hypothesis behind a relationship between the two conditions can be founded in (i) shared infectio ... | 2008 | 18517068 |
| in vitro evaluation of the effect of nicotine, cotinine, and caffeine on oral microorganisms. | the aim of this in vitro study was to evaluate the effects of nicotine, cotinine, and caffeine on the viability of some oral bacterial species. it also evaluated the ability of these bacteria to metabolize those substances. single-species biofilms of streptococcus gordonii, porphyromonas gingivalis, or fusobacterium nucleatum and dual-species biofilms of s. gordonii -- f. nucleatum and f. nucleatum -- p. gingivalis were grown on hydroxyapatite discs. seven species were studied as planktonic cell ... | 2008 | 18535638 |
| pcr-based identification of selected pathogens associated with endodontic infections in deciduous and permanent teeth. | the aim of the present study was to evaluate the presence of the selected pathogens in samples from deciduous and permanent tooth root canals by using pcr method and to determine the association of these organisms with clinical symptoms. | 2008 | 18547832 |
| coaggregation between and among human intestinal and oral bacteria. | coaggregation is believed to facilitate the integration of new bacterial species into polymicrobial communities. the aim of this study was to investigate coaggregation between and among human oral and enteric bacteria. stationary phase cultures of 10 oral and 10 enteric species, chosen on the basis of numerical and ecological significance in their respective environments together with their ease of cultivation, were tested using a quantitative spectrophotometric coaggregation assay in all possib ... | 2008 | 18554305 |
| experimental evidence supports the abscess theory of development of radicular cysts. | the objective of this study was to experimentally induce inflammatory cysts in an animal model so as to test the hypothesis that radicular cysts develop via the "abscess pathway." | 2008 | 18554947 |
| [relationship between herpesviruses and periodontal pathogenic bacteria in subgingival plaque]. | to evaluate the prevalence rates of human cytomegalovirus(hcmv) and epstein-barr virus-1(ebv-1) in subgingival plaque and analyze the relationship between herpesviruses, periodontal pathogenic bacteria and periodontal clinical parameters in chinese patients with aggressive periodontitis(agp). | 2008 | 18560463 |
| rapid and simple detection of eight major periodontal pathogens by the loop-mediated isothermal amplification method. | loop-mediated isothermal amplification (lamp) was applied to develop a rapid and simple detection system for eight periodontal pathogens: aggregatibacter (actinobacillus) actinomycetemcomitans, campylobacter rectus, eikenella corrodens, fusobacterium nucleatum, porphyromonas gingivalis, prevotella intermedia, treponema denticola and tannerella forsythia. primers were designed from the 16s ribosomal rna gene for each pathogen, and the lamp amplified the targets specifically and efficiently under ... | 2008 | 18565108 |
| differential gender effects of a reduced-calorie diet on systemic inflammatory and immune parameters in nonhuman primates. | dietary manipulation, including caloric restriction, has been shown to impact host response capabilities significantly, particularly in association with aging. this investigation compared systemic inflammatory and immune-response molecules in rhesus monkeys (macaca mulatta). | 2008 | 18565132 |
| effects of er:yag laser irradiation on biofilm-forming bacteria associated with endodontic pathogens in vitro. | with the development of dental laser delivery systems that can enter into the root canals, it is possible to use er:yag lasers to remove the residual biofilm associated with infected root canals. we examined their effects against biofilms made of actinomyces naeslundii, enterococcus faecalis, lactobacillus casei, propionibacterium acnes, fusobacterium nucleatum, porphyromonas gingivalis, or prevotella nigrescens in vitro. after er:yag laser irradiation with energy densities ranging between 0.38- ... | 2008 | 18570988 |
| comparative analysis of endodontic pathogens using checkerboard hybridization in relation to culture. | the purpose of this study was to detect bacterial species and to quantify the total number of bacteria from samples of infected root canals before (s1) and after chemo-mechanical preparation using 2% chlorhexidine (chx) gel as auxiliary chemical substance (s2) and after 7 days of intracanal dressing (s3) to compare microbial changes. | 2008 | 18582327 |
| periodontal status of patients with dentin dysplasia type i: report of three cases within a family. | dentin dysplasia type i (ddi) is a rare hereditary disturbance of dentin formation. it is characterized by clinically normal-appearing crowns; obliteration of pulp chambers; and short, blunted and malformed roots that are commonly associated with periodontal attachment loss (pal). in this context, we report three cases within a family with similar clinical and radiographic features of ddi but with differing microbiologic and periodontal conditions. | 2008 | 18597615 |
| biochemical characterization of a glycosyltransferase homolog from an oral pathogen fusobacterium nucleatum as a human glycan-modifying enzyme. | bacterial glycosyltransferases have drawn growing attention as economical enzymes for oligosaccharide synthesis, with their easy expression and relatively broad substrate specificity. here, we characterized a glycosyltransferase homolog (fnu_gt) from a human oral pathogen, fusobacterium nucleatum. bioinformatic analysis showed that fnu_gt belongs to the glycosyltransferases family ii. the recombinant fnu_gt (rfnu_gt) expressed in escherichia coli displayed the highest glycosylation activity when ... | 2008 | 18633282 |
| third molars and the efficacy of mechanical debridement in reducing pathogen levels in pregnant subjects: a pilot study. | to assess the impact of mechanical debridement (scaling and root planing), without adjunctive therapy, on reducing the numbers of periodontal pathogens detected in pregnant subjects with and without visible third molars. | 2008 | 18634941 |
| protein-lipid interactions with fusobacterium nucleatum major outer membrane protein foma: spin-label epr and polarized infrared spectroscopy. | foma, the major outer membrane protein of fusobacterium nucleatum, was expressed and purified in escherichia coli and reconstituted from detergent in bilayer membranes of phosphatidylcholines with chain lengths from c(12:0) to c(17:0). the conformation and orientation of membrane-incorporated foma were determined from polarized, attenuated total reflection, infrared (ir) spectroscopy, and lipid-protein interactions with foma were characterized by using electron paramagnetic resonance (epr) spect ... | 2008 | 18642853 |
| [study of adherence of periodontal pathogens to collagen-treated hydroxyapatite]. | the ability of oral bacteria to adhere to tooth surface is associated with their pathogenicity. the objective of this study was to compare the ability of 4 strains of periodontal pathogens attaching to collagen-treated hydroxyapatite (c-ha) beads in order to evaluate the ability of the main periodontal pathogens to form the biofilm on root surface. | 2008 | 18705525 |
| antiviral, antibacterial and vitamin c-synergized radical-scavenging activity of sasa senanensis rehder extract. | sasa senanensis rehder extract (se) showed slightly higher cytotoxicity against human squamous cell carcinoma cell lines and human glioblastoma cell lines, as compared with human oral normal cells (gingival fibroblast, pulp cell, periodontal ligament fibroblast), and was more cytotoxic to human myelogenous and t-cell leukemia cell lines. se showed a bacteriostatic effect on fusobacterium nucleatum and prevotella intermedia, but almost completely eliminated hydrogen sulfide (h2s) and methyl merca ... | 2008 | 18712174 |
| the influence of mineral trioxide aggregate on adaptive immune responses to endodontic pathogens in mice. | this study assessed the influence of mineral trioxide aggregate (mta) on adaptive immune responses. balb/c mice were immunized with heat-killed fusobacterium nucleatum (fn) in mta or other control adjuvants, and serum igg responses to fn were measured. either fn- or peptostreptococcus anaerobius (pa)-reactive memory t cells (tm) were preincubated in vitro with/without mta and restimulated with fn or pa. tm proliferation and cytokine production were assessed. compared with control groups, immunog ... | 2008 | 18718367 |
| rapid quantification of periodontitis-related bacteria using a novel modification of invader plus technologies. | the invader plus technology is a sensitive, rapid method for the detection and quantification of nucleic acid. while the original technology is based on the amplification by polymerase chain reaction (pcr) of the target sequence followed by its detection using the invader technology, the current modification allows simultaneous pcr amplification and invader reaction. the pcr primers and the invader probes are designed to operate at the same temperature. this allows simpler design and faster resu ... | 2010 | 18718748 |
| laboratory maintenance of fusobacteria. | this unit describes routine laboratory handling of fusobacteria. different media that can be used to grow or enrich fusobacterium nucleatum and other species of this genus are described. the growth and stock conditions as well as the susceptibility of f. nucleatum to oxygen in a pure culture are also discussed. | 2006 | 18770577 |
| photodynamic therapy as an adjunct to non-surgical periodontal treatment: a randomized, controlled clinical trial. | recent preclinical and clinical data have suggested a potential benefit of photodynamic therapy (pdt) in the treatment of periodontitis. however, there are very limited data from controlled clinical trials evaluating the effect of pdt in the treatment of periodontitis. the aim of this study was to evaluate the clinical and microbiologic effects of the adjunctive use of pdt to non-surgical periodontal treatment. | 2008 | 18771363 |
| in vitro bactericidal activity of recombinant human beta-defensin-3 against pathogenic bacterial strains in human tooth root canal. | human beta-defensin-3 (hbd3), an endogenous antimicrobial peptide, has strong broad-spectrum antimicrobial activity. this study aimed to obtain recombinant hbd3 (rhbd3) and to test the hypothesis that the antimicrobial characteristics of hbd3 may offer an advantage over conventional medicine in reducing intracanal bacteria. genetic engineering was used to obtain active rhbd3 and analysis revealed that it exhibited a broad spectrum of antibacterial activity at low micromolar concentrations agains ... | 2009 | 18775647 |
| identification of a methioninase inhibitor, myrsinoic acid b, from myrsine seguinii lév., and its inhibitory activities. | a methioninase inhibitor from myrsine seguinii was purified and identified as myrsinoic acid b. its inhibitory activities as to crude methioninase from periodontal bacteria such as fusobacterium nucleatum, porphyromonas gingivalis, and treponema denticola were determined. the ic50 values were 10.5, 82.4, and 30.3 microm respectively. | 2008 | 18776663 |
| [characterization and antibacterial effect of ag-nha-ntio2/polyamide 66 nanocomposite membrane on oral bacteria]. | undried silver-hydroxyapatite-titania (ag-nha-ntio2) nanoparticles slurry was used to make membrane with polyamide 66 (pa66) by co-polymerization method. the purpose of this study is to test the physical and chemical characteristics and antibacterial ability. | 2008 | 18780488 |
| [bacteremia after periodontal procedures]. | bacteremia frequently occurs after oral surgery and odontology procedures. periodontitis may affect the incidence and bacterial spectrum of bacteremia. periodontal disease may be a significant risk factor for the development of certain systemic diseases. this study has aimed to evaluate the frequency of aerobic and anaerobic bacteria in the bloodstream following scaling and root planing. | 2008 | 18792815 |
| the role of toll-like receptors 2 and 4 on reactive oxygen species and nitric oxide production by macrophage cells stimulated with root canal pathogens. | periapical lesions arise as a result of the activation and interaction of the host immune responses against root canal infection. recently identified toll-like receptors (tlr) seem to be involved in the recognition and development of immune responses against a myriad of microorganisms. however, very little information is available on the role of tlr in the induction of periapical lesions. | 2008 | 18793356 |
| rapid identification of oral anaerobic bacteria cultivated from subgingival biofilm by maldi-tof-ms. | to facilitate the identification of anaerobes cultivated from periodontal disease, whole cell bacterial identification by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (maldi-tof-ms) was evaluated. | 2008 | 18793359 |
| molecular identification and quantification of bacteria from endodontic infections using real-time polymerase chain reaction. | it was the aim of the present study to evaluate root canal samples for the presence and numbers of specific species as well as for total bacterial load in teeth with chronic apical periodontitis using quantitative real-time polymerase chain reaction (pcr). | 2008 | 18793361 |
| stimulation of epithelial cell matrix metalloproteinase (mmp-2, -9, -13) and interleukin-8 secretion by fusobacteria. | bacterial pathogens involved in periodontal diseases exert their destructive effects primarily by stimulating the host cells to increase their secretion of proinflammatory cytokines and matrix metalloproteinases (mmps). this study aimed to determine the epithelial cell matrix metalloproteinase and interleukin-8 (il-8) secretion upon exposure to fusobacteria. | 2008 | 18793368 |
| fusobacterial infections: clinical spectrum and incidence of invasive disease. | clinically significant infections caused by members of the genus fusobacterium are rare. we sought to describe the spectrum of clinical disease and epidemiology of these conditions presenting to an acute hospital over a five year period. | 2008 | 18805588 |
| effects of low-energy shock waves on oral bacteria. | we have recently demonstrated that extracorporeal shock-wave therapy (eswt) is effective in promoting the healing of dermal wounds and in regenerating alveolar bone lost through periodontal disease. the objective of the present study was to determine any antibacterial effect of eswt on oral bacteria. monoculture suspensions of 6 bacterial species were treated with 100 to 500 pulses of eswt at energy flux densities (efd) of 0.12 mj/mm(2), 0.22 mj/mm(2), and 0.3 mj/mm(2). following treatment, aliq ... | 2008 | 18809745 |
| olfactory perception of cysteine-s-conjugates from fruits and vegetables. | volatile sulfur compounds have a low odor threshold, and their presence at microgram per kilogram levels in fruits and vegetables influences odor quality. sensory analysis demonstrates that naturally occurring, odorless cysteine- s-conjugates such as s-( r/ s)-3-(1-hexanol)- l-cysteine in wine, s-(1-propyl)- l-cysteine in onion, and s-(( r/ s)-2-heptyl)- l-cysteine in bell pepper are transformed into volatile thiols in the mouth by microflora. the time delay in smelling these volatile thiols was ... | 2008 | 18811169 |
| [multiple brain abscesses of odontogenic origin caused by fusobacterium nucleatum]. | infection caused by the anaerobic gram-negative rod fusobacterium nucleatum belongs to endogenous infections. a 41-year-old man developed multiple brain abscesses of odontogenic origin. the main causes were poor oral hygiene and no dental check-ups. pus aspired from a site in the brain contained dna of the bacterium fusobacterium nucleatum. several teeth with periapical abscesses were extracted. initially, the patient was treated empirically with a combination of cefotaxime and metronidazole (18 ... | 2008 | 18821484 |
| periodontal bacterial profiles in pregnant women: response to treatment and associations with birth outcomes in the obstetrics and periodontal therapy (opt) study. | a recent clinical trial (obstetrics and periodontal therapy [opt] study) demonstrated that periodontal therapy during pregnancy improved periodontal outcomes but failed to impact preterm birth. the present study evaluated seven target bacteria, aggregatibacter actinomycetemcomitans (previously actinobacillus actinomycetemcomitans), porphyromonas gingivalis, treponema denticola, tannerella forsythia (previously t. forsythensis), prevotella intermedia, campylobacter rectus, and fusobacterium nucle ... | 2008 | 18834241 |
| granulocyte chemotactic protein 2 (gcp-2/cxcl6) complements interleukin-8 in periodontal disease. | mucosal inflammatory responses are orchestrated largely by pro-inflammatory chemokines. the chemokine granulocyte chemotactic protein 2 (cxcl6) is involved in neutrophil recruitment and migration. previous studies have shown that granulocyte chemotactic protein 2 is up-regulated during mucosal inflammation (e.g. in inflammatory bowel disease), similarly to the functionally and structurally related chemokine interleukin-8. nevertheless, unlike interleukin-8, a role of granulocyte chemotactic prot ... | 2009 | 18842116 |
| comparison of culture methods and multiplex pcr for the detection of periodontopathogenic bacteria in biofilm associated with severe forms of periodontitis. | conventional culture methods and multiplex pcr, both of which we have been used for a long time in our clinical microbiology laboratory, were compared for their ability to detect a selected panel of periodontopathic bacteria: aggregatibacter actinomycetemcomitans, campylobacter rectus, eikenella corrodens, fusobacterium nucleatum, porphyromonas gingivalis, and prevotella intermedia. tests were performed in a single subgingival sample taken from a periodontal diseased site with a probing depth eq ... | 2008 | 18843894 |
| dark field studies on oral spirochetes and fusobacterium plauti-vincenti before and after oral calcium penicillin medication. | 1948 | 18875169 | |
| streptococcus cristatus attenuates fusobacterium nucleatum-induced interleukin-8 expression in oral epithelial cells. | oral epithelial cells may be invaded by a polymicrobial intracellular flora, including pathogens together with commensals. various oral pathogens can induce the production of interleukin-8, a potent neutrophil chemotractant, in oral epithelial cells. evidence from the gut suggests that commensal species may modulate inflammatory responses to pathogens. the aim of this study was to examine the interleukin-8 responses of oral epithelial cells to an oral pro-inflammatory species, fusobacterium nucl ... | 2008 | 18942189 |
| participation of glutathione in the elimination of porphyromonas gingivalis in vivo. | glutathione is involved in immune responses such as cell proliferation and bactericidal activity. the aim of this study was to see whether glutathione influences the intraperitoneal elimination of porphyromonas gingivalis in fusobacterium nucleatum-immunized mice. | 2008 | 18954348 |
| uncultivated bacteria as etiologic agents of intra-amniotic inflammation leading to preterm birth. | intra-amniotic infection and inflammation are major causes of preterm birth (ptb). however, intra-amniotic inflammation is often detected in the absence of infection. this may partly be due to the culturing methods employed in hospital laboratories, which are unable to detect the uncultivated species. in this study, intra-amniotic microbial infections associated with ptb were examined by both culture and 16s rrna-based culture-independent methods and were corroborated by the presence of intra-am ... | 2009 | 18971361 |
| factors affecting human supragingival biofilm composition. i. plaque mass. | little is known about the factors that affect the microbial composition of supragingival biofilms. this study was designed to examine the relationship between total dna probe counts of supragingival biofilm samples, clinical parameters and supragingival biofilm composition. | 2009 | 18973540 |
| crystal structure of fada adhesin from fusobacterium nucleatum reveals a novel oligomerization motif, the leucine chain. | many bacterial appendages have filamentous structures, often composed of repeating monomers assembled in a head-to-tail manner. the mechanisms of such linkages vary. we report here a novel protein oligomerization motif identified in the fada adhesin from the gram-negative bacterium fusobacterium nucleatum. the 2.0 angstroms crystal structure of the secreted form of fada (mfada) reveals two antiparallel alpha-helices connected by an intervening 8-residue hairpin loop. leucine-leucine contacts pla ... | 2009 | 18996848 |
| naturally occurring phenolic antibacterial compounds show effectiveness against oral bacteria by a quantitative structure-activity relationship study. | natural and synthetic phenolic compounds were evaluated against oral bacteria. a quantitative structure-active relationship approach was applied to the germ-kill activity for a range of phenolic compounds. the lipophilicity and steric effects were found to be two key factors in determining germ-kill activity. the optimum lipophilicity, measured by the logarithm of the octanol/water partition coefficient, or log p, was found to be 5.5 for fusobacterium nucleatum , a gram-negative type of oral bac ... | 2008 | 19007234 |
| the fusobacterium nucleatum outer membrane protein radd is an arginine-inhibitable adhesin required for inter-species adherence and the structured architecture of multispecies biofilm. | a defining characteristic of the suspected periodontal pathogen fusobacterium nucleatum is its ability to adhere to a plethora of oral bacteria. this distinguishing feature is suggested to play an important role in oral biofilm formation and pathogenesis, with fusobacteria proposed to serve as central 'bridging organisms' in the architecture of the oral biofilm bringing together species which would not interact otherwise. previous studies indicate that these bacterial interactions are mediated b ... | 2009 | 19007407 |
| frequency, microbial interactions, and antimicrobial susceptibility of fusobacterium nucleatum and fusobacterium necrophorum isolated from primary endodontic infections. | this study assessed the prevalence and microbial interactions of fusobacterium nucleatum and fusobacterium necrophorum in primary endodontic infections from a brazilian population and their antimicrobial susceptibility to some antibiotics by the e-test. one hundred ten samples from infected teeth with periapical pathologies were analyzed by culture methods. five hundred eighty individual strains were isolated; 81.4% were strict anaerobes. f. nucleatum was found in 38 root canals and was associat ... | 2008 | 19026872 |
| oral bacteria modulate invasion and induction of apoptosis in hep-2 cells by pseudomonas aeruginosa. | pseudomonas aeruginosa is an important opportunistic bacterial pathogen, causing infections of the respiratory and other organ systems in susceptible hosts. p. aeruginosa infection is initiated by adhesion to and invasion of mucosal epithelial cells. the failure of host defenses to eliminate p. aeruginosa from mucosal surfaces results in p. aeruginosa proliferation, sometimes followed by overt infection and tissue destruction. there is growing evidence that associates poor oral health and respir ... | 2009 | 19041936 |
| fusobacterium nucleatum enhances invasion of human gingival epithelial and aortic endothelial cells by porphyromonas gingivalis. | invasion by porphyromonas gingivalis has been proposed as a possible mechanism of pathogenesis in periodontal and cardiovascular diseases. porphyromonas gingivalis have direct access to the systemic circulation and endothelium in periodontitis patients by transient bacteremia. periodontitis can be described as one of the predominant polymicrobial infections of humans. in the present study, p. gingivalis strains were tested for their ability to invade a human gingival epithelial cell line (ca9-22 ... | 2008 | 19049647 |
| coaggregation of treponema denticola with porphyromonas gingivalis and fusobacterium nucleatum is mediated by the major outer sheath protein of treponema denticola. | coagreggation of treponema denticola with either porphyromonas gingivalis or fusobacterium nucleatum was characterized and the role of the major outer sheath protein (msp) in the coaggregation process of these bacteria was evaluated. the msp of t. denticola was found to be able to bind to p. gingivalis and f. nucleatum cells and this binding could be inhibited by msp in a concentration-dependent manner. while sodium dodecyl sulfate polyacrylamide gel electrophoresis and periodic acid-schiff (pas ... | 2008 | 19054094 |
| characterization of coaggregation of fusobacterium nucleatum pk1594 with six porphyromonas gingivalis strains. | coaggregation is a key mechanism in biofilm formation. the aim of this study was to characterize the coaggregation of fusobacterium nucleatum pk1594 with six porphyromonas gingivalis strains in terms of kinetics and sugars inhibition. this coaggregation was quantitatively characterized by using a kinetic coaggregation assay. sugar inhibition profiles were also quantitatively defined. four types of interactions among these coaggregation partners were found: (1) fast coaggregation that was substan ... | 2009 | 19084124 |
| enhanced attachment of porphyromonas gingivalis to human fibroblasts mediated by fusobacterium nucleatum. | fusobacterium nucleatum and porphyromonas gingivalis are often co-isolated from endodontic infection sites. the present study tested the hypothesis that adhesin-mediated reactions might contribute to this phenomenon. f. nucleaum pk1594 and p. gingivalis pk1924 were selectively labeled with fluorochromes and allowed to adhere to human fibroblasts, either each strain alone or sequentially. the number of bacteria of each type adhering to individual fibroblasts was determined. sugar inhibition profi ... | 2009 | 19084131 |
| synergistic pathogenicity of porphyromonas gingivalis and fusobacterium nucleatum in the mouse subcutaneous chamber model. | porphyromonas gingivalis and fusobacterium nucleatum are often coisolated from sites of infection, such as suppurative apical periodontitis. the synergistic pathogenicity of mixed infection of p. gingivalis hg 405 with f. nucleatum pk 1594 was studied in the mouse subcutaneous chamber model in groups of seven animals. the minimal dose for p. gingivalis hg 405 that was required to infect 100% of the chambers was reduced by 1,000-fold when animals were inoculated in the same chamber with 1 x 10(9) ... | 2009 | 19084132 |
| proteolytic activities of oral bacteria on prommp-9 and the effect of synthetic proteinase inhibitors. | tissue reactions to bacteria lead to proinflammatory reactions involving matrix metalloproteinases (mmps). synthetic protease inhibitors may offer new possibilities to regulate bacterial proteases. we investigated proteolytic activities of certain periodontal bacteria, their effects on the latent prommp-9, and the effects of synthetic mmp inhibitors and a serine protease inhibitor pefabloc. the strains studied were porphyromonas gingivalis, prevotella intermedia, peptostreptoccus micros, prevote ... | 2008 | 19088890 |
| tinidazole inhibitory and cidal activity against anaerobic periodontal pathogens. | the in vitro activity of tinidazole against anaerobic periodontal pathogens (25 prevotella buccae, 18 prevotella denticola, 10 prevotella intermedia, 6 prevotella melaninogenica, 5 prevotella oralis, 10 fusobacterium nucleatum and 8 veillonella spp.) was determined by agar dilution. mic(90) values (minimum inhibitory concentration for 90% of the organisms) were 8 microg/ml for veillonella spp., 4 microg/ml for p. intermedia, 2 microg/ml for p. buccae, 1 microg/ml for fusobacterium spp. and 0.5 m ... | 2009 | 19097755 |
| [detection of periodontal pathogenic bacteria dna in coronary atheromatous plaques from patients underwent coronary artery bypass graft]. | the aim of this study was to observe the coexistence of periodontal bacteria dna (actinobacillus actinomycetemcomitans, fusobacterium nucleatum, porphyromonas gingivalis, prevotella intermedia, tannerella. forsythensis) in coronary atheromatous plaques and subgingival plaques in patients scheduled for coronary artery bypass graft. | 2008 | 19099976 |
| toll-like receptor 2 and nalp2 mediate induction of human beta-defensins by fusobacterium nucleatum in gingival epithelial cells. | we previously reported that infection by fusobacterium nucleatum strongly induced the expression of both human beta-defensin 2 (hbd-2) and hbd-3 by gingival epithelial cells. the aim of this study was to characterize the pattern recognition receptors (prrs) and regulatory mechanisms involved in the induction of hbd-2 and -3 expression by f. nucleatum in gingival epithelial cells. immortalized human gingival epithelial hok-16b cells were infected with live or heat-killed f. nucleatum, and the exp ... | 2009 | 19103770 |
| phenotypic and genotypic analyses of clinical fusobacterium nucleatum and fusobacterium periodonticum isolates from the human gut. | fusobacterium nucleatum is a gram-negative anaerobic rod that is part of the normal human microflora, and has also been associated with various infections. bacterial strains belonging to the species are typically heterogeneous in both phenotype and genotype, which can hinder their identification in a clinical setting. the majority of f. nucleatum isolates originate from oral sites, however the species is also a resident of the human gastrointestinal tract. the aim of this study was to compare f. ... | 2008 | 19114111 |
| periodontal pathogen levels in adolescents before, during, and after fixed orthodontic appliance therapy. | this purpose of this study was to document and investigate changes in periodontal pathogen levels before, during, and after orthodontic treatment in adolescents. | 2009 | 19121507 |
| short-term clinical and microbiological evaluations of peri-implant diseases before and after mechanical anti-infective therapies. | the aim of this study was to evaluate the clinical and microbiological effects of mechanical anti-infective therapies for mucositis and peri-implantitis. | 2009 | 19126114 |
| the microbiota of acute apical abscesses. | as the breadth of bacterial diversity in the oral cavity has been deciphered by molecular studies, several newly identified species/phylotypes have emerged as potential pathogens. we hypothesized that many of these species/phylotypes could also be involved with the etiology of endodontic abscesses. abscess aspirates from 42 persons were analyzed for the presence of 81 species/phylotypes by means of a reverse-capture checkerboard hybridization assay. associations between the most frequently detec ... | 2009 | 19131319 |
| [bacteriological analysis of subgingival plaque in adolescents]. | to investigate the changes in characteristics of periodontal pathogens in subgingival plaque of patients with puberty gingivitis and its relevance with clinical symptoms. | 2008 | 19134350 |
| a novel vaccine targeting fusobacterium nucleatum against abscesses and halitosis. | an abscess in a gum pocket, resulting from bacterial infection, is a common source of chronic halitosis. although antibiotics are generally prescribed for abscesses, they require multiple treatments with risks of creating resistant bacterial strains. here we develop a novel vaccine using ultraviolet-inactivated fusobacterium nucleatum (f. nucleatum), a representative oral bacterium for halitosis. a gum pocket model, established by continuous inoculation of f. nucleatum, was employed to validate ... | 2009 | 19162109 |
| new bacterial compositions in root-filled teeth with periradicular lesions. | the aim of this study was to isolate and detect microorganisms of root-filled teeth associated with periradicular lesions. specimens were sampled from patients undergoing root canal retreatment. the bacteria were characterized by morphologic and biochemical analysis and by 16s rrna gene sequencing. microorganisms were detected in 10 of 18 teeth. the majority of positive samples revealed a mixed culture of 2-8 species. in 2 teeth enterococcus faecalis was the only detected species. for the first ... | 2009 | 19166766 |
| coenzyme recognition and gene regulation by a flavin mononucleotide riboswitch. | the biosynthesis of several protein cofactors is subject to feedback regulation by riboswitches. flavin mononucleotide (fmn)-specific riboswitches, also known as rfn elements, direct expression of bacterial genes involved in the biosynthesis and transport of riboflavin (vitamin b(2)) and related compounds. here we present the crystal structures of the fusobacterium nucleatum riboswitch bound to fmn, riboflavin and antibiotic roseoflavin. the fmn riboswitch structure, centred on an fmn-bound six- ... | 2009 | 19169240 |
| endodontic microorganism susceptibility by direct contact test. | the aim of this study was to evaluate in vitro the duration of the antimicrobial effect of endodontic sealers by means of the direct contact test. the sealers tested were: endomethasone - septodont, endomethasone c-septodont, endion-voco, diaket-espe, pulp canal sealer-sybronendo, and ah26-dentsply detrey. the endodontopathic microorganisms (mo) confronted were: staphylococcus aureus (sa), candida albicans (ca), enterococcus faecalis (ef), prevotella intermedia (pi), porphyromonas gingivalis (pg ... | 2008 | 19177855 |
| effect of xylitol on an in vitro model of oral biofilm. | the aim of the present study was to examine whether xylitol, at different concentrations, inhibits the formation of an experimental model of oral biofilm. | 2008 | 19178100 |
| effect of dietary omega-3 polyunsaturated fatty acids on experimental periodontitis in the mouse. | periodontitis is an infective disease caused predominantly by gram-negative anerobes. the host inflammatory response to these bacteria causes alveolar bone loss, which characterizes periodontitis. omega-3 polyunsaturated fatty acids have recognized anti-inflammatory effects; their oxygenated derivatives are key mediators in reducing inflammation. in this study we tested the hypothesis that dietary supplementation with tuna fish oil rich in the n-3 polyunsaturated fatty acid, docosahexaenoic acid ... | 2009 | 19210341 |
| does pregnancy have an impact on the subgingival microbiota? | we investigated clinical and subgingival microbiologic changes during pregnancy in 20 consecutive pregnant women > or =18 years not receiving dental care. | 2009 | 19228092 |
| purulent pericarditis in patient with esophageal cancer presenting with cardiac tamponade. | 2009 | 19272728 | |
| an in vitro evaluation of hydrolytic enzymes as dental plaque control agents. | the plaque-control potential of commercially available amylase, lipase and protease was evaluated by observing their effects on coaggregation and on bacterial viability within various plaque microcosms. a quantitative coaggregation assay indicated that protease significantly inhibited the extent of coaggregation of actinomyces naeslundii and streptococcus oralis (p <0.05) and of porphyromonas gingivalis and s. oralis. amylase significantly (p <0.05) increased the coaggregation of a. naeslundii v ... | 2009 | 19273645 |
| fusobacterium nucleatum atcc 10953 requires actinomyces naeslundii atcc 43146 for growth on saliva in a three-species community that includes streptococcus oralis 34. | formation of dental plaque is a developmental process involving initial and late colonizing species that form polymicrobial communities. fusobacteria are the most numerous gram-negative bacteria in dental plaque, but they become prevalent after the initial commensal colonizers, such as streptococci and actinomyces, have established communities. the unusual ability of these bacteria to coaggregate with commensals, as well as pathogenic late colonizers, has been proposed to facilitate colonization ... | 2009 | 19286780 |
| tlr2 sensing of f. nucleatum and s. sanguinis distinctly triggered gingival innate response. | gingival tissue faces constant exposure to micro-organisms. it functions as part of the host response, an anti-microbial barrier that recognizes and discriminates between commensal and pathogenic bacteria. this study aimed to evaluate and compare the effects of cell wall extracts from different periodontal bacteria, commensals streptococcus sanguinis and fusobacterium nucleatum and the pathogen porphyromonas gingivalis, on the innate immune response of gingival keratinocytes and the role of tlr2 ... | 2009 | 19299164 |
| characterization of the complex bacterial communities colonizing biliary stents reveals a host-dependent diversity. | this study provides a comprehensive survey of the spatial and temporal bacterial composition of biliary stent biofilms. the bacterial diversity, distribution and dynamics of 59 biliary and 4 pancreatic stent communities from 40 patients being treated at two different hospitals, which implant stents either simultaneously or consecutively, were characterized by single-strand conformation polymorphism (sscp) analysis. fifty-one phylotypes belonging to 5 bacterial phyla and 24 bacterial families wer ... | 2009 | 19360025 |
| bacterial culture and dna checkerboard for the detection of internal contamination in dental implants. | the aim of this in vitro study was to evaluate the bacterial leakage along the implant-abutment interface by the conventional bacterial culture and dna checkerboard hybridization method. | 2009 | 19374709 |
| evidence for pseudoknot formation of class i preq1 riboswitch aptamers. | all in a knot. the smallest riboswitch forms a pseudoknot in solution. this is demonstrated for preq(1) class i aptamers by mutational analysis in combination with (1)h nmr-based structure probing. how pseudoknot formation mediates the mrna response through its expression platform is now open for investigation. | 2009 | 19382115 |
| are putative periodontal pathogens reliable diagnostic markers? | periodontitis is one of the most common chronic inflammatory diseases. a number of putative bacterial pathogens have been associated with the disease and are used as diagnostic markers. in the present study, we compared the prevalence of oral bacterial species in the subgingival biofilm of generalized aggressive periodontitis (gap) (n = 44) and chronic periodontitis (cp) (n = 46) patients with that of a periodontitis-resistant control group (pr) (n = 21). the control group consisted of subjects ... | 2009 | 19386852 |
| the oxyr homologue in tannerella forsythia regulates expression of oxidative stress responses and biofilm formation. | tannerella forsythia is an anaerobic periodontal pathogen that encounters constant oxidative stress in the human oral cavity due to exposure to air and reactive oxidative species from coexisting dental plaque bacteria as well as leukocytes. in this study, we sought to characterize a t. forsythia orf with close similarity to bacterial oxidative stress response sensor protein oxyr. to analyse the role of this oxyr homologue, a gene deletion mutant was constructed and characterized. aerotolerance, ... | 2009 | 19389765 |