Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| interrelationship between drug resistance and bacteriocinogeny of clostridium perfringens. | one hundred and eight strains of clostridium perfringens were collected from polluted waters and tested for their drug resistance and bacteriocinogeny . thirty of the strains were tetracycline resistant and 36 were bacteriocinogenic. only one strain possessed both tetracycline resistance and bacteriocinogen . transfer experiments on tetracycline resistance and bacteriocinogeny were carried out with several selected strains among these isolates. both tetracycline resistance and bacteriocinogeny w ... | 1984 | 6330503 |
| treatment of anaerobic infections. | the anaerobic syndromes of gas gangrene and tetanus are due to systemic intoxications that develop following infection with clostridia at some site of trauma. classically, these conditions developed as complications of accidental trauma, the spore-forming anaerobes being derived from the inanimate environment. while tetanus remains typically an exogenously derived disease, gas gangrene more frequently presents as an endogenously derived complication of elective surgery. their treatment is briefl ... | 1984 | 6330874 |
| [coccidiosis during the rearing of broiler breeding stock in limburg]. | increasing complaints concerning the rearing of broiler breeding stock in the province of limburg were the reason for collecting data and examining the possibilities of prevention more closely. the first batches of broiler breeders submitted to the animal health service in heythuysen were examined for the presence of coccidiosis throughout the year 1982. of 363 groups submitted, 1,484 birds about which there were complaints, were examined. of these, 153 flocks were found to be positive for cocci ... | 1984 | 6330928 |
| alternative medium for clostridium perfringens sporulation. | a medium containing 0.50 g of thiotone peptone, 0.30 g of soluble starch, 0.02 g of mgso4 x 7h2o, 0.90 g of na2hpo4 x 2h2o, 100.00 ml of distilled water, and optionally , 166 micrograms of dichloridric thiamine supported sporulation of 138 out of 141 clostridium perfringens strains. comparatively this medium gave a greater percentage of sporulation than five other media described previously. | 1984 | 6331307 |
| induction of ornithine decarboxylase in guinea-pig lymphocytes and its relation to phospholipid metabolism. | treatment of lymphocytes with exogenous phospholipase c (phosphatidylcholine cholinephosphohydrolase, ec 3.1.4.3.) derived from clostridium perfringens at concentrations similar to those which induced ornithine decarboxylase (l-ornithine carboxy-lyase, ec 4.1.1.17) activity produced diacylglycerol and phosphatidate. a divalent cation ionophore, a23187, and phytohemagglutinin induced not only diacylglycerol formation, but also ornithine decarboxylase activity. dibutyryl camp inhibited both diacyl ... | 1984 | 6331522 |
| anaerobic bacteria and beta-lactam antibiotics. | this review is concerned with anaerobic bacteria and beta-lactam antibiotics. data on the susceptibility to beta-lactam antibiotics of anaerobic isolates in sweden during 1983 is presented. among the different bacterial groups, clostridium perfringens, anaerobic cocci and curved rods were found to be very susceptible to the beta-lactams tested. c. difficile was susceptible to benzylpenicillin, piperacillin and imipenem, but resistant to the second and third generation of cephalosporins. most fus ... | 1984 | 6335601 |
| [microbiological study of spices. i. oregano]. | a total of 150 oregano samples from 6 different trade marks were studied. these samples were obtained at retail shops in buenos aires city and they were analyzed in order to determine the presence of the following microorganisms: 1) mesophilic aerobic plate count; 2) molds; 3) coliforms and fecal coliforms; 4) enterococci; 5) aerobes sporeforming: a) mesophilic aerobes; a1) total aerobes; a2) mesophilic anaerobes; b) thermophilic; b1) sulfide spoilage sporeformers; b2) total aerobes; b3) thermo- ... | 1984 | 6336363 |
| proteolysis of sialoglycoprotein by pasteurella haemolytica cytotoxic culture supernatant. | proteolytic enzyme activity releasing sialo glycopeptides from 3h-labeled human erythrocyte ghosts was detected in cytotoxic (leukotoxic) culture supernatants from 9 of 12 pasteurella haemolytica serotypes. microcrystalline cellulose thin-layer chromatograms of radioactive water-soluble products showed the following two radioactive peaks: a high-mobility minor peak (rf, 0.54 to 0.74), identified as sialic acid, and a low-mobility major peak (rf, 0.18 to 0.21), partially characterized as a trichl ... | 1983 | 6352504 |
| anaerobic bacteria as cause of infections in female genital organs. | anaerobic bacteria constitute a substantial component of the normal vaginal flora and of the outer cervical canal. consequently, one would expect infections emanating from the vaginal flora to be caused to a substantial degree by anaerobic bacteria. the anaerobes may contribute in colpitis, but their role is difficult to prove in this situation, since sampling only yields normal flora components. one clue that anaerobes may contribute to colpitis is the circumstance that the flora under those co ... | 1983 | 6353553 |
| comparison of three methods for anaerobe identification. | in this study we evaluated the ability of three commercial methods, api 20a (analytab products, plainview, n.y.), minitek (bbl microbiology systems, cockeysville, md.), and anaerobe-tek (flow laboratories, inc., mclean, va.), to accurately identify 165 recent clinical and 38 stock isolates of anaerobic bacteria without supplemental tests or gas-liquid chromatography. strains included 89 bacteroides spp., 12 fusobacteria, 10 gram-positive, nonsporing rods, 43 clostridium spp., 15 streptococcus in ... | 1983 | 6355150 |
| anaerobic infections in childhood. | anaerobic bacteria are part of the normal flora of mucous membranes and outnumber aerobic bacteria in the oral cavity and gastrointestinal tract. anaerobes can be isolated from pediatric patients with various infections when appropriate techniques for transportation and cultivation of samples are employed. frequently anaerobes are isolated in combination with other facultative or aerobic bacteria. the genera or groups of anaerobes most frequently isolated from pyogenic infections in children are ... | 1984 | 6372028 |
| recovery of anaerobic bacteria from vented blood-culture bottles. | from january 1979, to july 1981, blood from each patient suspected of having bacteremia was collected in two bottles of commercially prepared tryptic soy broth; one bottle was vented for 24 hr to the atmosphere. bottles were incubated at 37 degrees c for a maximum of seven days and were examined daily for signs of growth. blind subcultures onto chocolate-agar plates were performed 18-24 hr after collection and again at 48 hr. for 63,106 bottles inoculated, a total of 4,788 strains were isolated, ... | 1984 | 6372038 |
| human platelet activation by bacterial phospholipase c is mediated by phosphatidylinositol hydrolysis but not generation of phosphatidic acid: inhibition by a selective inhibitor of phospholipase c. | we have shown earlier that phospholipase c (plc) from clostridium perfringens causes human platelet aggregation and secretion in a concentration dependent manner. the present study was undertaken to further characterize the specificity of the effects of plc and to better understand the mechanism of the action of this inducer. a methylene-dioxybenzazepine (mdba) analog of trimetoquinol was synthesized and tested for antiplatelet activity. mdba (3-30 microm) inhibited plc-induced aggregation in a ... | 1984 | 6372157 |
| [recent advances in the molecular mechanism of action of bacterial toxins, in particular of diphtheria, cholera, coli, botulinum and shigella toxins as well as tetanospasmin and the toxins of staphylococcus aureus]. | great progress was achieved in the clarification of the molecular structure and the mechanism of action of the toxins of pathogenic forms of bacteria. proportions of toxins of corynebacterium diphtheriae and of pseudomonas aeruginosa transfer from the nad and adp-ribose protein to an amino acid of the elongation factor 2. thus the protein synthesis is much inhibited. the cholera toxin and the l-toxin from escherichia coli have a similar structure. they transfer an adp-ribose portion from nad to ... | 1984 | 6375175 |
| rapid glutamic acid decarboxylase test for identification of bacteroides and clostridium spp. | a rapid 4-h test for glutamic acid decarboxylase is described for the identification of certain anaerobic bacteria. the test substrate consisted of 1.0 g of l-glutamic acid, 0.3 ml of triton x-155, and 0.05 g of bromcresol green sodium salt in 1 liter of water. the substrate was dispensed in 0.5-ml amounts into test tubes, and a turbid suspension was made with the test organism. the test was then incubated aerobically at 35 degrees c for 4 h. the development of a blue color was considered positi ... | 1984 | 6376535 |
| evaluation of minitec and api as rapid diagnostic methods for anaerobic bacteria. | the performance of the miniaturized biochemical differentiation systems api 20 anaerobe system and minitek was compared with ph reactions in pras media of 58 anaerobic or capno-aerophilic bacterial strains from recent clinical specimens. the overall correlation of the minitek reactions was 87% and of the api reactions 85%. the reactions of acid formation from carbohydrates was slightly higher for minitek. this resulted in a correct diagnosis in 90% after minitek and 86% after api. if corrected f ... | 1984 | 6377470 |
| assay of metronidazole by hplc compared with microbial method. | a high-performance liquid chromatography (hplc) method for the assay of metronidazole and its 2-hydroxymethyl metabolite in sera was compared with a microbiological method, an agar well diffusion technique with clostridium perfringens as indicator strain. the hplc technique involves separation of metronidazole from its two active major metabolites (the 2-hydroxymethyl and the 1-carboxymethyl derivatives) on a mu-bondapak c18 column and uv detection at 313 nm. the mobile phase was 35% acetonitril ... | 1984 | 6377472 |
| evaluation of the elisa as tool in diagnosing clostridium perfringens enterotoxins. | detecting clostridium perfringens enterotoxin (cpe) using the enzyme linked linked immunosorbent assay (elisa) was evaluated as a tool for diagnosing enterotoxicosis caused by c. perfringens. this method was assessed using a number of different food poisoning outbreaks with possible c. perfringens associations. cpe can easily be detected in faeces of patients involved in food-borne disease caused by c. perfringens. in stools of patients with diarrhoea 0.01-10 micrograms/g of cpe is detectable, h ... | 1984 | 6382878 |
| effect of erythromycin on microbial antagonisms: a study in gnotobiotic mice associated with a human fecal flora. | the effect of erythromycin base was studied on intestinal resistance to colonization of gnotobiotic mice inoculated with a human fecal flora and challenged with six microbial strains potentially pathogenic for immunocompromised patients. fecal concentrations of erythromycin were greater than 1,000 micrograms/g in the human donor and in mice. total intestinal bacterial counts were not significantly different in the human donor and in the recipient mice and were not affected by erythromycin treatm ... | 1983 | 6413596 |
| bacillus cereus-induced myonecrosis. | a patient with an incomplete amputation due to a crush injury to his arm developed a myonecrosis with gram-positive rods noted on muscle and wound aspirates. the patient was treated for a probable clostridium perfringens infection but culture results proved the organism to be bacillus cereus. in light of the reported resistance of bacillus cereus to penicillin, this case serves to emphasize the importance of expanded empiric coverage with high-dose penicillin and an aminoglycoside pending the re ... | 1984 | 6423833 |
| an enzyme-linked immunosorbent assay for the detection of clostridium perfringens enterotoxin antibody. | an enzyme-linked immunosorbent assay (elisa) was adapted to test serum antibody to enterotoxin of clostridium perfringens type a. the test was evaluated using sheep, calf and guinea pig sera and compared with passive hemagglutination and immunodiffusion tests. the elisa was found to be more sensitive than the other two tests and was completely free from nonspecific reactions. the method was considered to be technically advantageous and suitable for semiautomated procedures. | 1984 | 6424913 |
| presence of a nonlysosomal endo-beta-n-acetylglucosaminidase in the cellular slime mold dictyostelium discoideum. | vegetative cells of the cellular slime mold dictyostelium discoideum have been found to contain an endo-beta-n-acetylglucosaminidase (ec 3.2.1.96) activity which hydrolyzes the di-n-acetylchitobiosyl linkage found in asparagine-linked oligosaccharides. in contrast to other previously characterized glycosyl hydrolases of dictyostelium, this endoglycosidase is not secreted during vegetative growth or development nor is it developmentally regulated. cellular fractionation studies showed that the en ... | 1984 | 6430241 |
| in vitro and in vivo antibacterial activities of mt-141, a new semisynthetic cephamycin, compared with those of five cephalosporins. | the in vitro and in vivo antibacterial activities of mt-141 were compared with those of cefoxitin, cefmetazole, moxalactam, cefotaxime, and cefoperazone. the mics of mt-141 for 90% of bacterial isolates were lower than the reference drugs against clinical isolates of campylobacter jejuni, clostridium difficile, and bacteroides fragilis, whereas against clinical isolates of other gram-positive, gram-negative, and anaerobic bacteria, the mics of mt-141 were similar to or higher than those of the r ... | 1984 | 6440478 |
| [in vitro activity of the amoxicillin-clavulanic acid combination on anaerobic bacteria. comparison with amoxicillin, lamoxactam and metronidazole]. | minimal inhibitory concentrations (mic) of the combination amoxicillin + clavulanic acid (aac) (2/1 w/w) on 84 strains of obligate anaerobes were measured by the agar diffusion method and compared to those of amoxicillin (amo), lamoxactam (lam) and metronidazole (met). for the 47 bacteroides fragilis strains tested, the mode mic was 0.5 microgram/ml with aac, 1-2 with met, 0.5-1 with lam and 8-16 with amo. a similar range of activity was found on the 16 other strains of bacteroides sp.. aac and ... | 1984 | 6462743 |
| necrotizing enterocolitis in very low birthweight infants: a four-year experience. | fifty (13%) of 375 infants who weighed 1500 g or less at birth had necrotizing enterocolitis (nec). haematological changes suggestive of sepsis occurred in 83% and positive bacteriological cultures were found in 38%, the most common organism isolated being clostridium perfringens. complications included intestinal perforation in six patients and recurrence of nec in five, of whom one subsequently developed an intestinal stricture. five of the eight nursery deaths were secondary to peritonitis an ... | 1984 | 6466213 |
| acute diarrhea in horses of the potomac river area: examination for clostridial toxins. | fecal specimens from horses in montgomery county, md, and in fairfax and loudoun counties, va, were examined for clostridium perfringens type a enterotoxin and for c difficile cytotoxin (92 and 108 specimens, respectively). the toxins were found in feces from horses that had experienced an acute diarrhea syndrome and from clinically normal horses. the toxins did not appear to be primary determinants of the diarrhea syndrome, although they may have contributed to the spectrum of clinical entities ... | 1984 | 6469842 |
| a transient increase in diacylglycerols is associated with the action of vasopressin on hepatocytes. | vasopressin induced a transient increase of 50% in the total concentration of diacylglycerols (determined by g.l.c.) in isolated hepatocytes. the increase was maximal at 0.25 min, and the concentration of diacylglycerols in cells treated with vasopressin had returned to the basal value by 4 min. no change in the concentration of diacylglycerols was observed after the treatment of cells with glucagon. the dependency of this effect on the concentration of vasopressin was similar to that of the eff ... | 1984 | 6477530 |
| more on necrotizing enterocolitis: pigbel in papua new guinea. | 1984 | 6482927 | |
| osmotic stabilizers differentially inhibit permeability alterations induced in vero cells by clostridium perfringens enterotoxin. | using a sensitive vero (african green monkey kidney) cell model system, studies were performed to further investigate whether clostridium perfringens enterotoxin acts via disruption of the colloid-osmotic equilibrium of sensitive cells. enterotoxin was shown to cause a rapid loss of intracellular 86rb+ (mr approx. 100) with time- and dose-dependent kinetics. the enterotoxin-induced release of intracellular 86rb+ preceded the loss of two larger labels, 51cr label (mr approx. 3500) and 3h-labeled ... | 1984 | 6487622 |
| [the effect of nutrition on intestinal flora]. | in several groups of probands the influence of nutrition on the gut flora has been examined. in old people there is a striking decrease in the numbers of bifidobacteria whereas those of clostridium perfringens strongly increase and those of enterobacteriaceae and lactobacilli can be found somewhat higher than in the compared group. in a group of longevitial old aged men nourished with a fibre-rich diet these alterations are found less striking. changing from a mixed japanese diet to a "western d ... | 1984 | 6493319 |
| empedopeptin (bmy-28117), a new depsipeptide antibiotic. i. production, isolation and properties. | empedopeptin is a new antibiotic produced by empedobacter haloabium nov. sp. (atcc 31962). it is a water-soluble depsipeptide antibiotic containing eight amino acid residues and a c14-fatty acid moiety in the molecule. although structurally unrelated, empedopeptin and vancomycin have similar antimicrobial spectra against aerobic and anaerobic gram-positive bacteria including antibiotic-resistant strains. empedopeptin is highly active in vivo in mice against systemic infections of staphylococcus ... | 1984 | 6501108 |
| biological assay for clostridium perfringens enterotoxin with vero cells. | 1984 | 6513244 | |
| antibiotic sensitivity testing of anaerobic bacteria by the breakpoint method. | a shortened form of the agar dilution procedure (breakpoint method) was studied for susceptibility testing of 363 strains of anaerobic bacteria under routine conditions. mezlocillin inhibited 99%, piperacillin 96%, cefoxitin 99%, latamoxef 90%, clindamycin 96% and metronidazole 100% of bacteriodaceae strains tested. peptococcaceae were susceptible to penicillins, cephalosporins and metronidazole. we did not find noticeable resistance of clostridium perfringens strains to beta-lactam antibiotics ... | 1984 | 6518836 |
| in vitro activity of rifaximin and rifampicin against some anaerobic bacteria. | activity of rifampicin and of a new rifamycin, rifaximin, was tested in strains of anaerobic bacteria belonging to the bacteroides genus (75 b. fragilis group and 17 bacteroides non-fragilis group) and in clostridium perfringens (15 strains). it turned out that the bactericidal activity of both rifamycins could be overlapped and that it equalled 100% in the case of the non-fragilis bacteroides and clostridium species. | 1984 | 6529778 |
| relationship between cell surface asparagine-linked glycoproteins and myoblast differentiation. analysis of wheat germ agglutinin-resistant mutants. | alterations in the oligosaccharides of complex surface glycoproteins have been studied in wild-type rat skeletal myoblasts (l6-9/1) and two wheat germ agglutinin resistant mutants, selected for either high level of resistance (wgari) or low level of resistance (wgarii). all three lines possessed high mannose oligosaccharides of the structure man(n)glcnac, where n = 6, 7, or 8. l6-9/1 had complex oligosaccharides of the type (+/- neunac alpha 2-3gal beta glcnac beta)n-man3glcnac(+/- fuc)glcnac wh ... | 1984 | 6546892 |
| decreased [20-3h]phorbol-12,13-dibutyrate binding to phospholipase c pretreated epidermal cells. | incubation of mouse epidermal cells (hel-37) with phospholipase c (clostridium perfringens) caused about a 50% decrease in the specific binding of [20-3h]phorbol-12,13-dibutyrate. phospholipase c caused a decrease in the apparent number of binding sites from 2.86 x 10(5) to 1.21 x 10(5) per cell with little effect on ligand affinity. the decrease was observed with purified phospholipase containing no detectable protease activity, and when cells were fixed with glutaraldehyde. the phorbol-12,13-d ... | 1983 | 6573950 |
| the activity of enoxacin against clinical bacterial isolates in comparison with that of five other agents, and factors affecting that activity. | the activity of enoxacin against 362 clinical bacterial isolates in comparison with norfloxacin, nalidixic acid, ampicillin, latamoxef (moxalactam) and gentamicin was tested by an agar dilution method. typical mics for enterobacteria lay between 0.12 and 1.0 mg/l. enterobacter spp. and serratia spp. tended to be more resistant. enoxacin was also active against pseudomonas aeruginosa (mean mic 0.5 mg/l) and highly active against fastidious gram-negative aerobes. typical mics for staphylococcus au ... | 1984 | 6594324 |
| activation of the calcium permeability of erythrocyte membranes by perfringolysin o. | calcium ions inhibited perfringolysin o-induced hemolysis at a concentration lower than 1 mm, but not the hemolysis by digitonin at 10 mm. the introduction of calcium ions into ghosts inhibited the lysis more strongly than the addition of calcium ions outside ghosts. when erythrocytes were treated with perfringolysin o in the presence of 1 mm cacl2 containing 45cacl2, the radioactivities inside cells rapidly increased during incubation. on the other hand, when perfringolysin o-treated erythrocyt ... | 1983 | 6619118 |
| cefamandole-erythromycin-heparin peritoneal irrigation: an adjunct to the surgical treatment of diffuse bacterial peritonitis. | the enthusiasm for the use of peritoneal irrigation has waxed and waned since its introduction by dr. pierce in 1905. the purpose of this study was to devise a relatively low-cost irrigant that could be used for continuous intraperitoneal lavage, with the intent of decreasing abscess formation following surgical treatment for generalized bacterial peritonitis. a solution of 1 l of normal saline containing 50 mg erythromycin, 50 mg cefamandole, 500 u heparin, and 5 meq kcl was proven in in vitro ... | 1983 | 6623358 |
| freeze-dried mixed cultures as samples for proficiency tests and collaborative studies in food microbiology. | a method is presented for preparing samples of freeze-dried mixtures of microorganisms for proficiency tests and collaborative studies. the samples may include most microorganisms that are found in routine analysis in food laboratories. transport of samples during 48 h did not decrease the number of microorganisms, nor was the variability among samples significantly affected by transport. the standard deviation of counts after 5 weeks of storage varied from 0.04 (staphylococcus aureus) to 0.17 ( ... | 1983 | 6643366 |
| detection of d-erythro and l-threo sphingosine bases in preparative sphingosylphosphorylcholine and its n-acylated derivatives and some evidence of their different chemical configurations. | sphingosylphosphorylcholine prepared from native sphingomyelin by the kaller procedure was found to comprise about 70% of the l-threo (2s, 3s) isomer and 30% of the d-erythro (2s, 3r) isomer. this analytical result was obtained by gas-liquid chromatography (glc) of trimethylsilyl derivatives of n-acetylsphingosines which were prepared by enzymatic hydrolysis of synthetic n-acetylsphingosylphosphorylcholines with clostridium perfringens phospholipase c. some other evidence of the different chemic ... | 1983 | 6654881 |
| [anaerobic effect of tinidazol compared with metronidazol, ornidazol, cefoxitin and lamoxactam]. | the in vitro inhibitory activity of tinidazole, metronidazole, ornidazole, cefoxitin and moxalactam was determined against 150 isolates of clinically important anaerobes including bacteroides fragilis, bacteroides bivius and clostridium perfringens by means of agar dilution tests. the members of 18 gramnegative and 14 grampositive species were inhibited by tinidazole at less than or equal to 0,01-8 micrograms/ml thus being without exception susceptible to the drug. a similar in vitro activity wa ... | 1983 | 6676177 |
| in vitro activity of u-57930e against anaerobic bacteria and its comparison with clindamycin, ampicillin, carbenicillin and tetracycline. | the in vitro activity of u-57930e, a pipecolic acid amide of clindamycin, was compared with those of clindamycin, ampicillin, carbenicillin and tetracycline against 321 anaerobic clinical isolates. the mic (micrograms/ml) of u-57930e that inhibited 95% bacteroides fragilis, peptococcus prevotii, b. melaninogenicus and p. asaccharolyticus was 0.0625; 0.03125 for peptostreptococcus anaerobius, b. vulgatus, propionibacterium and peptococcus species. clindamycin, on the other hand, gave mic values o ... | 1983 | 6678915 |
| [efficacy of tinidazole against anaerobes in comparison with metronidazole, ornidazole, cefoxitin and lamoxactam]. | the in vitro inhibitory activity of tinidazole, metronidazole, ornidazole, cefoxitin and moxalactam was determined against 150 isolates of clinically important anaerobes including bacteroides fragilis, bacteroides bivius and clostridium perfringens by means of agar dilution tests. the members of 18 gramnegative and 14 grampositive species were inhibited by tinidazole at less than or equal to 0,01-8 micrograms/ml thus being without exception susceptible to the drug. a similar in vitro activity wa ... | 1983 | 6681175 |
| phospholipids chiral at phosphorus. preparation and spectral properties of chiral thiophospholipids. | the thiophospholipid 1,2-dipalmitoyl-sn-glycero-3-thiophosphocholine (dppsc) was shown to be a mixture of two diastereomers by 31p nuclear magnetic resonance. the isomer that resonates at the lower field in cdcl3 (56.12 ppm) was designated as isomer a and the other (resonates at 56.07 ppm) as isomer b. phospholipase a2 from four different sources (bee venom, naja naja venom, crotalus adamanteus venom, and porcine pancreas) was shown to hydrolyze the isomer b of dppsc specifically, whereas phosph ... | 1983 | 6688028 |
| effects of deglycosylation on the architecture of ovine submaxillary mucin glycoprotein. | the structural features of native and deglycosylated ovine submaxillary mucin (osm) were determined by electron microscopy of platinum unidirectionally shadowed preparations and by ultracentrifugation. thin filamentous molecules, of which 90% were 100-230 nm in length with estimated diameters of 1.0-1.4 nm, were observed with dilute samples of osm in high ionic strength solvents (5-30 micrograms/ml in 0.8 m nacl or nh4ac). ultracentrifugation studies indicated that these filamentous structures w ... | 1984 | 6699011 |
| the significance of anaerobic bacteria in biliary tract infection after hepatic portoenterostomy for biliary atresia. | aspiration of the biliary atrium was performed in six children who developed cholangitis after hepatic portoenterostomy (kasai procedure). all aspirates were cultured for aerobic and anaerobic bacteria. aerobic bacteria were recovered in all six specimens and anaerobic organisms were recovered in three. the predominant aerobic organisms were klebsiella pneumoniae (four isolates), enterococcus (three isolates, and escherichia coli (two isolates). the anaerobes recovered were bacteroides fragilis ... | 1984 | 6701784 |
| comparative in vitro activities of cefpiramide and apalcillin against anaerobic bacteria. | the in vitro activities of two new antimicrobial agents, apalcillin and cefpiramide (sm-1652), were evaluated against 324 strains of anaerobic bacteria. apalcillin (a penicillin derivative) and cefpiramide (a semisynthetic cephalosporin) were compared with piperacillin, moxalactam, and cefoxitin. organisms studied included the bacteroides fragilis group, other bacteroides species, fusobacteria, clostridia, nonsporeforming gram-positive rods, and anaerobic cocci. piperacillin was found to be the ... | 1984 | 6712200 |
| [gas gangrene--still a diagnostic and therapeutic problem]. | from 1970 to 1980 132 patients were admitted to our dept. under the diagnosis of gas gangrene. in 54 cases there was no bacteriological evidence of clostridium perfringens. in all patients surgery was performed immediately, followed by hyperbaric oxygen therapy. the overall mortality rate among 78 patients with proven gas gangrene came up to 38%, the amputation after limb injuries to 55%. in our experience we can not state a clear cut advantage of hyperbaric oxygenation as far as the death rate ... | 1984 | 6720134 |
| bile acid deconjugation and attachment of chicken gut bacteria: their possible role in growth depression. | bacteria isolated from the chicken gut were tested for their ability to deconjugate bile acids and attach to chicken epithelial cells (crop squamous cells and duodenal brush borders). clostridium perfringens, streptococci and some of the bifidobacteria and lactobacilli were able to deconjugate all 4 substrates whereas the bacteroides deconjugated only the taurine conjugates and the coliforms were completely inactive. none of the strains of escherichia coli or streptococci attached to squamous ce ... | 1984 | 6733554 |
| some nutritional aspects of colic in horses. | consistency of exercise and diet are important in colic prevention. water should be offered before and after feeding. fast-growing foals suckling heavily lactating mares may overeat grain at weaning. creep feeding to accustom the foal to eating grain and gradually increasing the grain intake after weaning are helpful in preventing colic in foals. stallions may overeat grain when taken off pasture in hot weather. feeding hay initially and grain later helps avoid colic in these stallions. type-d c ... | 1984 | 6738503 |
| gas gangrene of the omental bursa following acute pancreatitis. | acute pancreatitis with necrosis was followed by infection with clostridium perfringens and formation of gas in the omental bursa. this could be seen preoperatively on an abdominal roentgenogram and was verified at operation. the patient recovered after debridement, drainage and irrigation and antimicrobial therapy. | 1984 | 6740250 |
| enrichment medium and control system for isolation of campylobacter fetus subsp. jejuni from stools. | enrichment culture with a semisolid medium increased by 6% the isolation rate of campylobacter fetus subsp. jejuni. the semisolid enrichment medium was also used successfully as a transport medium for campylobacter isolates. a blood agar plate streaked with pseudomonas aeruginosa, clostridium perfringens, and a laboratory strain of campylobacter was a good control system for the microaerophilic atmosphere. good growth of all three organisms indicated satisfactory conditions for culturing campylo ... | 1982 | 6764764 |
| isolation and structural characterization of human lymphocyte and neutrophil gangliosides. | gangliosides were isolated from purified preparations of human peripheral blood lymphocytes and neutrophils. structural analyses and comparisons were performed by direct probe mass spectrometry and by degradation studies with the following enzymes: escherichia freundii endo-beta-galactosidase; clostridium perfringens and arthrobacter ureafaciens neuraminidase; and jack bean beta-n-acetylhexosaminidase and beta-galactosidase. this combination of techniques allowed us to obtain carbohydrate compos ... | 1981 | 6780555 |
| immunodiffusion method for detection of type a clostridium botulinum. | a simple gel immunodiffusion agar procedure was developed for detecting toxigenic strains of clostridium botulinum type a. the method consisted of overlaying colonies grown on thin-layer tryptone-peptone-glucose-yeast extract agar with gel diffusion agar containing desired levels of c. botulinum type a antitoxin. concentric precipitin zones formed around colonies of c. botulinum type a. strains of c. botulinum type a were detected by this procedure. however, c. botulinum type b reacted to a less ... | 1981 | 6797350 |
| virginiamycin effects on controlling necrotic enteritis infection in chickens. | duplicate trials were conducted with male broiler chickens to evaluate virginiamycin as treatment against experimentally induced necrotic enteritis infection. each trial consisted of seven treatments, each replicated four times, with 10 birds per replicate. two treatments were fed control ration (noninfected control and infected control) and the five remaining treatments were fed virginiamycin at 5, 10, 15, 20, or 40 g/ton. birds were orally dosed with 10 ml of clostridium perfringens culture at ... | 1982 | 6806795 |
| binding of clostridium perfringens 125i-labeled delta-toxin to erythrocytes. | hemolytically active, 125i-labeled delta-toxin from clostridium perfringens was used to study the binding of this cytolysin to sheep, goat, human, rabbit, horse, mouse, and guinea pig erythrocytes. the extent of toxin binding was correlated with the known hemolytic specificity of the toxin. detailed studies of the binding were carried out on sheep erythrocytes which showed the highest sensitivity to lysis by delta-toxin. simultaneous determination of toxin binding and release of intracellular 86 ... | 1983 | 6822539 |
| clostridium perfringens cellulitis and immune-mediated hemolytic anemia in a horse. | a 10-year-old quarter horse mare was referred for evaluation and treatment of a large pectoral skin slough and hemoglobinuria. the skin slough was secondary to clostridium perfringens cellulitis and associated gas gangrene. cold hemagglutinin disease was diagnosed and was suspected to be secondary to c perfringens septicemia. the autoimmune hemolytic anemia, severe intravascular hemolysis, and hemoglobinuria were treated with dexamethasone and hydrocortisone. the infection was treated with 20 x ... | 1983 | 6826449 |
| bacteria in enteric lesions of cattle. | thirty-nine species of bacteria were isolated from or demonstrated in the abomasal and small and large intestinal mucosa of 23 adult cattle and 41 calves and identified. the bacteria isolated were related to the gross and microscopical lesions. campylobacters, clostridium perfringens type a, c sordellii, actinobacillus lignieresii, fusobacterium necrophorum, mycobacterium paratuberculosis, aeromonas hydrophila and escherichia coli were all associated with specific lesions. the relationship of ot ... | 1983 | 6829137 |
| photolabeling of staphylococcal alpha-toxin from within rabbit erythrocyte membranes. | intrinsic membrane proteins of rabbit red blood cells were labeled with the photoreactive amphipatic reagent 12-(4-azido-2-nitrophenoxy) stearoyl (1-14c) glucosamine, which inserts into the hydrophobic membrane region and generates a reactive nitrene upon ultraviolet irradiation. photolabeling of membrane-bound staphylococcal alpha-toxin after lysis of probe-treated rabbit red blood cells by this toxin implies its penetration into the hydrophobic region of the outer leaflet of the membrane. in c ... | 1983 | 6838569 |
| association of iota-like toxin and clostridium spiroforme with both spontaneous and antibiotic-associated diarrhea and colitis in rabbits. | a helically coiled, anaerobic, gram-positive sporeforming bacillus, identified as clostridium spiroforme, was isolated from the cecal contents of all of 27 rabbits with spontaneous diarrhea, at a mean concentration of 10(6.0) spores per g of material. all of these rabbits also had a toxin present in their cecal contents that was neutralized by anti-clostridium perfringens type e iota toxin, but not by other clostridial antitoxins. in addition, four rabbits with clindamycin-associated colitis wer ... | 1983 | 6841578 |
| myonecrotic gas gangrene of the extremities. | twenty-three patients with proven myonecrotic gas gangrene of the extremities caused by clostridium perfringens bacteria were treated by a combination of surgical measures, benzyl-penicillin and hyperbaric oxygen at three atmospheres. 87 per cent (20/23) of the cases were cured and half of the surviving patients (10) had to be amputated at least one level higher than on admission. no single factor could be established that influenced the course of the infection comparing age under and over 50 ye ... | 1983 | 6845998 |
| exogenous phospholipase enzymes mimic effects of phenylephrine on ca2+ transport in hepatocytes. | phospholipase c from clostridium perfringens induced the release of 45ca2+ from isolated rat hepatocytes incubated at 0.1 mm extracellular ca2+ with a time course similar to that for the action of phenylephrine. under the conditions of these experiments, no significant damage to the plasma membrane was detected in the presence of phospholipase c. little 45ca2+ release was induced by bee venom phospholipase a2. at 1.3 mm extracellular ca2+, both phospholipase enzymes stimulated the initial rate o ... | 1983 | 6847637 |
| effects of phospholipase c on the na+-ca2+ exchange and ca2+ permeability of cardiac sarcolemmal vesicles. | we have examined the effects of phospholipase c pretreatment on ca2+ transport in highly purified canine cardiac sarcolemmal vesicles. na+-ca2+ exchange, measured as nai+-dependent ca2+ uptake, is stimulated when 10-70% of the membrane phospholipid has been hydrolyzed. although the phospholipase c treatment also increases sarcolemmal passive ca2+ flux, the membrane maintains a sufficient permeability barrier for enhanced transport (via na+-ca2+ exchange) to be observed. these effects can be obta ... | 1983 | 6853558 |
| membrane-damaging action of alveolysin from bacillus alvei. | we investigated membrane damage to human diploid, embryonic lung fibroblasts caused by highly purified alveolysin by measuring leakage of cytoplasmic markers and uptake of various metabolites, and we observed a leakage pattern typical of sh-activated cytolysins. however, the mode of membrane interaction resembled the mode of membrane interaction of theta-toxin from clostridium perfringens rather than that of streptolysin o in the following respects: the activity on fibroblast membranes was high ... | 1981 | 6894743 |
| metronidazole: in vitro activity, pharmacology and efficacy in anaerobic bacterial infections. | metronidazole is a 5-nitroimidazole that has selective activity against anaerobic microorganisms, including bacteria and protozoa. intravenous metronidazole has recently been approved by the u.s. food and drug administration for the treatment of serious anaerobic bacterial infections. it is usually bactericidal at low concentrations, and its spectrum of activity encompasses almost all anaerobic bacteria and some capnophilic organisms. anaerobic bacteria known to be resistant to metronidazole inc ... | 1981 | 6927601 |
| nitroimidazoles: in vitro activity and efficacy in anaerobic infections. | nitroimidazoles, including metronidazole, tinidazole and ornidazole, are low molecular weight antimicrobial compounds with excellent activity against anaerobic microorganisms. these compounds are usually bactericidal at low concentrations and their spectrum of activity encompasses almost all the anaerobic bacteria and some capnophylic organisms. the few anaerobic bacteria known to be resistant to the nitroimidazoles include occasional anaerobic cocci, some nonsporing gram positive bacilli and pr ... | 1981 | 6941456 |
| n-formimidoyl thienamycin (mk0787): in vitro activity against anaerobic bacteria. | the in vitro activity of n-formimidoyl thienamycin (mk0787) was tested against 239 anaerobic bacteria clinical isolates: 70 of bacteroides fragilis, 18 of b. distasonis, 16 of b. thetaiotaomicron, 10 of b. vulgatus, 24 of bacteroides spp., 22 of b. melaninogenicus (all three subspecies), 26 of fusobacterium spp., 10 of peptococcus spp., 15 of peptostreptococcus spp., 15 of clostridium perfringens, and 13 of clostridium spp. ninety-five percent of the isolates were inhibited by less than or equal ... | 1982 | 6952818 |
| in vitro and in vivo activity of dl-8280, a new oxazine derivative. | dl-8280, 9-fluoro-3-methyl-10-(4-methyl-1-piperazinyl)-7-oxo-2,3-dihydro-7h- pyrido-(1,2,3-de)1,4-benzoxazine-6-carboxylic acid, is a new nalidixic acid analog with a broad spectrum of antibacterial activity against gram-negative and gram-positive bacteria, including obligate anaerobes. the activity of dl-8280 against enterobacteriaceae, pseudomonas aeruginosa, haemophilus influenzae, neisseria gonorrhoeae, and clostridium perfringens was roughly comparable to that of norfloxacin and far exceede ... | 1982 | 6960805 |
| photo-oxidation of l-glutamate decarboxylase from escherichia coli, sensitized by the coenzyme pyridoxal phosphate and by proflavin. | irradiation of l-glutamate decarboxylase (l-glutamate 1-carboxy-lyase, ec 4.1.1.15) from escherichia coli by visible light absorbed by the intrinsic chromophore, pyridoxal phosphate, caused the selective modification of two methionines per enzyme monomer. the disulfoxide derivative exhibited modified circular dichroism, chromatographic and kinetic properties, suggesting a conformational role for the two methionine residues. irradiation of the enzyme in the presence of proflavin revealed the pres ... | 1980 | 6990995 |
| studies on the sulfite reduction test for clostridia. | peptone-yeast extract (py) medium containing 0.035% ferric ammonium citrate as an indicator, 0.05% sulfite as a substrate, 0.05% cysteine as a reducer and 0.5% glucose was found to be suitable for observing the sulfite reduction test. the effect of added cysteine on the test was suppressed by the addition of glucose. in cultures of bacteria grown for 2 days at 37 c in medium containing the above ingredients, 121 among 132 strains of clostridia, including 86 strains of clostridium perfringens, ga ... | 1980 | 6993870 |
| k-1 antigen content and the presence of an additional sialic acid-containing antigen among bacteremic k-1 escherichia coli: correlation with susceptibility to opsonophagocytosis. | eighty percent of blood culture isolates of escherichia coli k-1 are resistant to in vitro opsonophagocytosis by normal human granulocytes and fresh serum. to determine the basis for susceptibility to phagocytosis in 20% of bacteremic k-1 e. coli, we investigated possible quantitative and qualitative immunochemical differences in the k-1 antigen content among resistant and sensitive isolates. we prepared extracts of blood culture k-1 e. coli by sonication and determined the k-1 polysaccharide co ... | 1980 | 7000702 |
| the clinical significance, taxonomy and special methodological problems of the pathogenic clostridia. | the clinical significance of clostridia is much greater than is generally recognized. the organisms are a major cause of septic abortion, clostridium perfringens being the most important single organism. this species is also the principle agent in food-poisoning. clostridium botulinum is considered to be one of the main causes of the sudden death syndrome in infants. as clostridia are universally distributed in nature and the human body, the isolation of an organism of this group from the human ... | 1980 | 7005089 |
| [anaerobic bacteria in clinical specimens of a hospital laboratory (author's transl)]. | over a 14-month period 261 clinical specimens were cultured anaerobically, this figure representing about 3% of the total specimens submitted to the microbiological laboratory. not accepted for anaerobic culture were stools, sputa, urines, vaginal and urethral swabs and swabs from superficial skin lesions. all materials were sent to the laboratory in an anaerobic transport medium and processed according to the recommendations of the american society for microbiology and the center for disease co ... | 1981 | 7020261 |
| the bacterial flora of neonates with congenital abnormalities of the gastro-intestinal tract. | the development of the bacterial flora of neonates with congenital abnormalities of the gastro-intestinal tract was studied in 31 infants during the first 10 days of life. specimens were collected from the umbilicus, mouth and gastro-intestinal tract on the pre-operative day, at operation and on post-operative days 1, 2, 3, 5, 7 and 10. bacteria were isolated semi-quantitatively on a variety of plain and selective media and identified by conventional methods.staphylococcus albus was the predomin ... | 1982 | 7057028 |
| fatal clostridium perfringens infection after normal term pregnancy. | we describe a case of fatal gas gangrene of the uterus after an apparently normal term pregnancy in a 22-year-old woman. the patient died less than 15 h after the onset of symptoms, which consisted of a single episode of vomiting and increasing hypogastric pain. hypotension, tachycardia and renal failure were the dominant clinical features. the pathogenesis, diagnosis and treatment of uterine gas gangrene are discussed. | 1982 | 7073337 |
| [necrotising enteritis (author's transl)]. | 4 cases of severe necrotising enteritis are dealt with. the small intestine was affected 3 times, the colon once, one patient died after resection of the affected bowel segment. in 2 additional cases a large resection of the small intestine became necessary. one patient was treated conservatively. the bacteriological evidence of clostridium perfringens type c in the intestinal wall allows the assumption of a primarily bacterial genesis of this disease. its aetiology, clinical and pathological-an ... | 1982 | 7102142 |
| [acute clostridium perfringens peritonitis in prolonged presence of a pessary]. | 1982 | 7106632 | |
| malignant oedema caused by clostridium perfringens type a in a horse. | 1982 | 7120271 | |
| pathogenic anaerobes. | anaerobes are prevalent on all mucosal surfaces and virtually all anaerobic infections are endogenous. two thirds of anaerobic infections involve five anaerobic organisms or groups--the bacteroides fragilis group, the bacteroides melaninogenicus-bacteroides asaccharolyticus group, fusobacterium nucleatum, the anaerobic cocci, and clostridium perfringens. conditions that lower the oxidation-reduction potential and disrupt the mucosal surface (eg, vascular problems, malignant neoplasms, and surger ... | 1982 | 7125789 |
| water disinfection with the hydrogen peroxide-ascorbic acid-copper (ii) system. | treatment of secondary effluents with hydrogen peroxide (10 mg/liter)-ascorbic acid (10 mg/liter)-cu2+ (0.5 mg/liter) for 60 min resulted in around 99% reduction of the initial plate count. hydrogen peroxide could be replaced by other peroxygen compounds; ascorbic acid could be replaced by other reducing agents, of which sodium sulfite and ethanol were the most effective. cu2+, however, could not be replaced by other metal ions without loss of bactericidal efficiency of the ternary combination. ... | 1982 | 7138000 |
| [binding of cationized ferritin to the surface of the palatine crest in the rat]. | recently, we suggested that the cell coat of the surface epithelial cells of the palatal shelves and especially its sialyl groups are implied in the adhesion process which initiates the constitution of the secondary palate. in this work, use of cationized ferritin which interacts with anionic receptors of the plasma membrane give us the possibility to render these sialic acids visible. after five minutes incubation in presence of cationized ferritin, the labeling particles are distributed in mon ... | 1982 | 7139138 |
| [gas gangrene caused by clostridium perfringens infection]. | 1982 | 7154794 | |
| biotransformation of 1-nitropyrene in intestinal anaerobic bacteria. | mutagenic nitroaromatic compounds have recently been found in photocopies, urban atmosphere, automobile exhaust and wastewater. 1-nitropyrene (1-np) is readily formed when pyrene, ubiquitous in the environment, is exposed to nitrogen dioxide in the urban atmosphere or in automobile exhaust, and is highly mutagenic, inducing 449 his+ revertants/plate/nmol from salmonella typhimurium strain ta98 in the absence of s9 fraction in the salmonella-microsome test. it is possible to swallow sputum or som ... | 1982 | 7167066 |
| age-development changes in susceptibility of erythrocytes to perfringolysin o. | susceptibility to perfringolysin o of erythrocytes from mice of different ages was examined. erythrocytes of mice younger than 5 weeks' old were more resistant to the toxin than those of young adult and adult mice. erythrocytes of aged mice were about 3.5 times more susceptible to the toxin than erythrocytes from 4-week-old mice. the membrane cholesterol content of erythrocytes appeared to be maintained at a constant level throughout the ages of mice examined. about 5% of the total membrane chol ... | 1982 | 7176704 |
| [microbiological studies of beef tartar]. | two hundred lots of 'filet américain' (a mixture of minced meat, acid sauce, condiments, salt, etc., meant to be eaten raw) were sampled from retailers and examined for several types of micro-organism; 185 lots had been prepared by the retailer, fifteen on an 'industrial' scale. pork had been used in seventy-three lots (including fourteen 'industrial' lots); beef was present in all lots, horse meat in none of them. on the whole, the bacterial state of the meats in which pork had been used was fo ... | 1982 | 7179231 |
| effective immunization of lambs against enterotoxaemia. | in contrast to adult sheep, 2- to 3-month-old lambs do not respond well to a single injection of clostridium perfringens type d oil adjuvant epsilon toxoid. this unresponsiveness can be overcome, however, by administering 2 injections of oil adjuvant vaccine or one injection of oil adjuvant followed 4 weeks later by an injection of alum-precipitated toxoid. the latter procedure evokes protective antitoxin levels which persist for 8 months, and a booster injection of alum-precipitated toxoid give ... | 1980 | 7231926 |
| [haemolysis as initial sign of clostridium perfringens septicaemia in newborn (author's transl)]. | cases of clostridium perfringens, septicaemia are subject to a very gloomy prognosis in adults. this applies likewise to the rare cases reported in paediatric literature. the present report describes the fatal case of a newborn with clostridium perfringens septicaemia. the principal sign was a severe haemolysis which failed to respond to blood exchange transfusions and antibiotic treatment. the characteristic changes of colour of the skin usually observed in septicaemia of newborn had probably b ... | 1981 | 7289436 |
| intrasplenic abscess: two case reports and review of the literature. | the occurrence of splenic abscess, sickle cell trait, and drug addiction is described in two patients. the coincidence of sickle cell disease and drug addiction in the possible etiology of splenic abscess should be noted. the first patient had a splenic abscess in the inferior pole of the spleen with signs and symptoms of left upper quadrant peritoneal irritation. the second patient had an intrasplenic abscess in the superior pole with pleuritic type chest pain and large left pleural effusion. i ... | 1981 | 7305137 |
| analysis of membrane structure in the transitional epithelium of rat urinary bladder. 3. localization of cholesterol using filipin and digitonin. | 1981 | 7310938 | |
| isolation and preliminary characterization of novel polysialogangliosides from embryonic chick brain. | preparative thin-layer chromatography was used to isolate from embryonic chick brains four different polysialogangliosides which moved below gt1b on tlc plates. these possessed molar sialic acid-to-sphingosine ratios of 4:1, 5:1, 6:1, and 7:1, respectively. mild neuraminidase (clostridium perfringens) treatment of the three most highly sialylated fractions gave gt1b and gd1b transiently, but the only accumulating ganglioside was gm1. all three polysialogangliosides were also found in elasmobranc ... | 1981 | 7320736 |
| comparative in vitro activity of new beta-lactam antibiotics against anaerobic bacteria. | several new beta-lactam antimicrobial agents have been introduced in the last few years. in this investigation, the in vitro activities of several recently introduced cephalosporins (cefoperazone, cefotaxime, ceftazidime, and ceftizoxime), moxalactam, and n-formimidoyl thienamycin were compared with those of cefoxitin, clindamycin, and metronidazole against 203 strains of anaerobic bacteria. at achievable serum levels, all of the antimicrobial agents were active against essentially 100% of the s ... | 1981 | 7325628 |
| the release of n-acetyl- and n-glycolloyl-neuraminic acid from soluble complex carbohydrates and erythrocytes by bacterial, viral and mammalian sialidases. | a series of substrates, sialyl(2 leads to 6)galnac and ganglioside gm3, containing either n-acetylneuraminic acid (acneu) or n-glycolloylneuraminic acid (gcneu), has been prepared. the trisaccharide gcneu(2 leads to 3)lactose was preapred by ozonolysis of gcneu-gm3, and the disaccharides acneu(2 leads to 6)galnac and gcneu(2 leads to 6)galnac were isolated from bovine submandibular-gland mucin by alkali elimination. sialidases from newcastle-disease virus, fowl-plague virus, influenza virus a2, ... | 1981 | 7325957 |
| [effect of the sporotoxin of clostridium perfringens (welchii) on isolated intestine of the rabbit]. | 1981 | 7348320 | |
| [gangrenous cholecystitis without lithiasis. a report on a case diagnosed by simple radiography of the abdomen (author's transl)]. | the authors report a case of gangrenous cholecystitis without lithiasis but with secondary infection of the gallbladder by anaerobic germs. pre-operative diagnosis was possible from simple x-rays of the abdomen which demonstrated an obvious fluid level in the gallbladder, and by parietography. an immediate cholecystectomy was performed. bacteriological examination of the bile confirmed the presence of clostridium perfringens. the main bile duct appeared normal and the bile in the common bile duc ... | 1980 | 7380889 |
| microflora and deconjugation of bile acids in alkaline reflux after partial gastrectomy. | it has been postulated that reflux of bile into the stomach promotes gastric carcinogenesis. bile-stained aspirates from 50 asymptomatic patients, partially gastrectomized more than 10 years earlier, were examined bacteriologically and with regard to conjugated and deconjugated bile acids. endoscopic biopsies showed atrophic gastritis in all patients, cancer in two and severe dysplasia in another two. ph in the reflux aspirates was 7.3 +/- 0.4 (mean +/- standard deviation). bacterial cultures w ... | 1980 | 7406138 |
| clostridium perfringens septicemia following cesarean section. | a clostridium perfringens infection is described in an 18-year-old primigravida following cesarean section at 43 weeks' gestation. despite the ubiquitous distribution of these organisms, such infections are rarely encountered in obstetrics. characteristics of these bacteria and appropriate diagnostic and therapeutic procedures are presented. despite the infrequency of this potentially lethal complication, survival depends on early diagnosis and aggressive treatment. | 1980 | 7422200 |
| [bacteriological and hygienic control of rendering plants (author's transl)]. | procedures for the hygienic inspection of animal rendering plants should include the bacteriological investigation of the meat meal, the bacteriological control of the used methods of disinfection of the contaminated rooms, of the transport vehicles and of the sewage of the contaminated side. meat meal has to be free of clostridium perfringens and salmonellae. clostridium perfringens is an indicator of the sterilizing effect of the rendering procedures. after an effective disinfection of rooms n ... | 1980 | 7424272 |
| effect of sialic acid removal on human low density lipoprotein catabolism in vivo. | this study was undertaken to determine whether sialic acid removal alters the catabolism of low density lipoprotein in humans. human low density lipoproteins labeled in vitro with 125i were incubated in the presence (termed desialylated) or absence (sialylated) of neuraminidase. the treatment with neuraminidase from clostridium perfringens removed 90% of the sialic acid residues which did not change the chemical composition of the lipoproteins. sialylated or desialylated ldl were injected intrav ... | 1980 | 7426100 |
| isolation and characterization of a novel monosialosylpentahexosyl ceramide from tay-sachs brain. | a novel monosialoganglioside was isolated from tay-sachs brains. it represented about 0.1% of the total ganglioside mixture. compositional analysis by gas-liquid chromatography indicated that it contained glucose, galactose, n-acetylgalactosamine, n-acetylneuraminic acid, and long chain base in the molar ratio of 1:2:2:1:1. the ganglioside was found to be resistant to neuraminidase (clostridium perfringens), beta-hexosaminidase (jack bean), and beta-galactosidase. however, it could be degraded b ... | 1981 | 7451432 |