Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| photodynamic therapy for endodontic disinfection. | the aims of this study were to investigate the effects of photodynamic therapy (pdt) on endodontic pathogens in planktonic phase as well as on enterococcus faecalis biofilms in experimentally infected root canals of extracted teeth. strains of microorganisms were sensitized with methylene blue (25 microg/ml) for 5 minutes followed by exposure to red light of 665 nm with an energy fluence of 30 j/cm2. methylene blue fully eliminated all bacterial species with the exception of e. faecalis (53% kil ... | 2006 | 16982278 |
| in vitro antimicrobial activity of sodium hypochlorite and chlorhexidine against selected single-species biofilms. | to investigate the antimicrobial activity of 2.5% and 5.25% sodium hypochlorite and 2.0% chlorhexidine gel and liquid as endodontic-irrigating substances against selected single-species biofilms. | 2006 | 17014526 |
| splenic vein thrombosis presenting as fusobacterium nucleatum bacteremia. | 2006 | 17022361 | |
| [multiple intracerebral lesions. identification of the causative agent by 16s rdna-pcr]. | molecular biological methods such as polymerase chain reaction (pcr) enable microbiologists to detect bacteria even if antibiotic treatment has already been started. based on this case report of a 39-year-old man with multiple intracerebral lesions, we describe a pcr method called 16s rdna-pcr which can be used to identify panbacterial dna by focussing on the universal gene sequences for the bacterial 16s part of the ribosome. | 2006 | 17063323 |
| carpegen real-time polymerase chain reaction vs. anaerobic culture for periodontal pathogen identification. | the aim of this study was to compare two methods of microbiological diagnosis, anaerobic bacterial culture and real-time polymerase chain reaction (pcr), for the detection of actinobacillus actinomycetemcomitans, porphyromonas gingivalis, prevotella intermedia, tannerella forsythia, fusobacterium nucleatum, and treponema denticola. | 2006 | 17064390 |
| amphipathic polymers: tools to fold integral membrane proteins to their active form. | among the major obstacles to pharmacological and structural studies of integral membrane proteins (mps) are their natural scarcity and the difficulty in overproducing them in their native form. mps can be overexpressed in the non-native state as inclusion bodies, but inducing them to achieve their functional three-dimensional structure has proven to be a major challenge. we describe here the use of an amphipathic polymer, amphipol a8-35, as a novel environment that allows both beta-barrel and al ... | 2006 | 17115690 |
| effects of sonicated prevotella intermedia, fusobacterium nucleatum and lactobacillus casei extracts on interleukin-8 production by human dental pulp cells. | the objective of this study was to determine the effects of prevotella intermedia, fusobacterium nucleatum and lactobacillus casei on the production of il-8 by human dental pulp cells. human dental pulp cells from teeth of young patients (aged 18-25 years) were cultured and tested with sonicated p. intermedia atcc 25611, f. nucleatum atcc 25586 and l. casei atcc 4646 extracts. il-8 secreted into the culture supernatants were measured at 6, 12 and 24 hours using a quantitative sandwich enzyme imm ... | 2006 | 17120973 |
| common antacids could help keep gingivitis at bay. | 2006 | 17128225 | |
| antimicrobial effect of acidified nitrate and nitrite on six common oral pathogens in vitro. | salivary nitrate is positively correlated with plasma nitrate and its level is 9 times the plasma level after nitrate loading. nitrate in saliva is known to be reduced to nitrite by oral bacteria. nitrate and nitrite levels in saliva are 3 - 5 times those in serum in physiological conditions respectively in our previous study. the biological functions of high salivary nitrate and nitrite are still not well understood. the aim of this in vitro study was to investigate the antimicrobial effects of ... | 2006 | 17134590 |
| crystallization and preliminary x-ray data of the fada adhesin from fusobacterium nucleatum. | fusobacterium nucleatum is a gram-negative anaerobe prevalent in the oral cavity that is associated with periodontal disease, preterm birth and infections in other parts of the human body. the bacteria attach to and invade epithelial and endothelial cells in the gum tissue and elsewhere via a 13.7 kda adhesin protein fada (fusobacterium adhesin a). fada exists in two forms: the intact form (pre-fada), consisting of 129 amino acids, and the mature form (mfada), which lacks an 18-residue signal se ... | 2006 | 17142900 |
| phylogenetic analysis of bacterial and archaeal species in symptomatic and asymptomatic endodontic infections. | phylogenetic analysis of bacterial and archaeal 16s rrna was used to examine polymicrobial communities within infected root canals of 20 symptomatic and 14 asymptomatic patients. nucleotide sequences from approximately 750 clones amplified from each patient group with universal bacterial primers were matched to the ribosomal database project ii database. phylotypes from 37 genera representing actinobacteria, bacteroidetes, firmicutes, fusobacteria and proteobacteria were identified. results were ... | 2007 | 17172525 |
| the proteomic profile of fusobacterium nucleatum is regulated by growth ph. | fusobacterium nucleatum is a saccharolytic gram-negative anaerobic organism believed to play an important role in the microbial succession associated with the development of periodontal disease. its genome contains niche-specific genes shared with the other inhabitants of dental plaque, which may help to explain its ability to survive and grow in the changing environmental conditions experienced in the gingival sulcus during the transition from health to disease. the ph of the gingival sulcus in ... | 2007 | 17185543 |
| clinical and microbiological profiles of human immunodeficiency virus (hiv)-seropositive brazilians undergoing highly active antiretroviral therapy and hiv-seronegative brazilians with chronic periodontitis. | this study compares the periodontal clinical profile and the composition of the subgingival microbiota of human immunodeficiency virus (hiv)-seropositive and hiv-seronegative subjects with chronic periodontitis. | 2007 | 17199544 |
| rat model of polymicrobial infection, immunity, and alveolar bone resorption in periodontal disease. | one of the predominant polymicrobial infections of humans is expressed clinically as periodontal disease. porphyromonas gingivalis, treponema denticola, and tannerella forsythia have been strongly implicated as members of a pathogenic consortium in the etiology of adult periodontitis. in this study we hypothesized that p. gingivalis, t. denticola, and t. forsythia are synergistic in terms of virulence potential and induce chronic periodontal inflammation that leads to alveolar bone resorption in ... | 2007 | 17210663 |
| hyperactivity and reactivity of peripheral blood neutrophils in chronic periodontitis. | some evidence exists that peripheral neutrophils from patients with chronic periodontitis generate higher levels of reactive oxygen species (ros) after fcgamma-receptor stimulation than those from healthy controls. we hypothesized that peripheral neutrophils in periodontitis also show both hyper-reactivity to plaque organisms and hyperactivity in terms of baseline, unstimulated generation and release of ros. peripheral neutrophils from chronic periodontitis patients and age/sex/smoking-matched h ... | 2007 | 17223966 |
| inflammation associated with implants with different surface types. | the aim of this study was to determine the nature of the inflammatory infiltrate associated with different transmucosal implant surfaces in dogs. | 2007 | 17224032 |
| sensitivity of oral bacteria to 254 nm ultraviolet light. | to explore the sensitivity of bacteria commonly found in root canals to 254 nm ultraviolet (uv) light, either as individual cells or as participants of a bacterial multilayer. | 2007 | 17229117 |
| antianaerobe activity of ceftobiprole, a new broad-spectrum cephalosporin. | agar dilution testing of 463 anaerobes showed most gram-positive beta-lactamase-negative strains (other than some clostridium difficile and peptostreptococcus anaerobius), as well as both beta-lactamase-positive and beta-lactamase-negative strains of fusobacterium nucleatum, to have ceftobiprole mic values of < or =0.016 to 4 microg/ml. ceftobiprole was less active against beta-lactamase-positive gram-negative bacilli, especially the members of the bacteroides fragilis group. like ceftobiprole, ... | 2007 | 17240107 |
| interleukin-1alpha stimulation in monocytes by periodontal bacteria: antagonistic effects of porphyromonas gingivalis. | periodontal pathogenic bacteria are associated with elevated levels of interleukin-1alpha (il-1alpha) but it is unclear if all species can induce cytokine production equally. porphyromonas gingivalis may be able antagonize il-1alpha induced by other species through the activity of its proteases or lipopolysaccharide (lps). monomac-6 cells and primary human monocytes were treated with culture supernatants from porphyromonas gingivalis, fusobacterium nucleatum, campylobacter rectus, actinobacillus ... | 2007 | 17241171 |
| actinobacillus actinomycetemcomitans lipopolysaccharide regulates matrix metalloproteinase, tissue inhibitors of matrix metalloproteinase, and plasminogen activator production by human gingival fibroblasts: a potential role in connective tissue destruction. | fibroblasts, a major constituent of gingival connective tissue, can produce immunoregulatory cytokines and proteolytic enzymes that may contribute to tissue destruction. in this study, we evaluated the production of matrix metalloproteinases (mmps), tissue inhibitors of mmps (timps), and plasminogen activators by gingival fibroblasts stimulated with lipopolysaccharides (lps) produced by periodontopathogens, including actinobacillus actinomycetemcomitans. in addition, changes in the expression an ... | 2007 | 17299802 |
| efficacy of gasiform ozone and photodynamic therapy on a multispecies oral biofilm in vitro. | ozone gas and photodynamic therapy (pdt) have been claimed to be antimicrobially effective. this study assessed their antimicrobial potential in vitro. mature six-species oral biofilms were treated as follows (n = 9 per group): (i) a 60-s application of gasiform vacuum-ozone or vacuum alone (on wet or air-dried biofilm samples); (ii) pdt (i.e. methylene blue in combination with or without a diode soft laser, and a soft laser alone); or (iii) antimicrobial solutions: immersion of biofilms for 60 ... | 2007 | 17305720 |
| gingival epithelial cell transcriptional responses to commensal and opportunistic oral microbial species. | transcriptional profiling and ontology tools were utilized to define the biological pathways of gingival epithelial cells modulated by coculture with the oral commensal streptococcus gordonii and the opportunistic commensal fusobacterium nucleatum. overall, f. nucleatum and s. gordonii perturbed the gingival epithelial cell transcriptome much less significantly than the oral pathogens porphyromonas gingivalis and aggregatibacter actinomycetemcomitans perturbed the transcriptome, indicating that ... | 2007 | 17307939 |
| microbiological outcomes of quadrant versus full-mouth root planing as monitored by real-time pcr. | to study the short-term microbiological changes following full-mouth compared with quadrant wise scaling and root planing (fmrp and qrp) as well as long-term effects. | 2007 | 17309590 |
| a comparison of implant-supported, bar- or ball-retained mandibular overdentures: a retrospective clinical, microbiologic, and immunologic study of 10 edentulous patients attending a recall visit. | clinical, microbiologic, and immunologic comparisons of the peri-implant health in edentulous volunteers wearing long-standing implant-supported ball- or dolder bar-retained mandibular overdentures were performed. | 2007 | 17319360 |
| efficacy of the de novo-derived antimicrobial peptide wlbu2 against oral bacteria. | the efficacy of a novel synthetic antimicrobial peptide (wlbu2) was evaluated against three oral microorganisms (grown planktonically): streptococcus gordonii, fusobacterium nucleatum, and porphyromonas gingivalis. wlbu2 killed all three species, with f. nucleatum being the most susceptible. wlbu2 also reduced the bacterial burden of s. gordonii and f. nucleatum biofilms. | 2007 | 17325219 |
| response to alkaline stress by root canal bacteria in biofilms. | to determine whether bacteria isolated from infected root canals survive alkaline shifts better in biofilms than in planktonic cultures. | 2007 | 17326786 |
| the detection of eight putative periodontal pathogens in adult and rapidly progressive periodontitis patients: an institutional study. | periodontal disease is a commonly prevalent problem faced alike by both the developed and third world countries but showing wide variations in prevalence and severity across different geographical areas. the purpose was to identify actinobacillus actinomycetemcomitans (aa), porphyromonas gingivalis (pg), prevotella intermedia (pi), ekinella corrodens (ec), campylobacter rectus (cr), bacteroides forsythus (bf), treponema denticola (td) and fusobacterium nucleatum (fn) in indian adult periodontiti ... | 2007 | 17347537 |
| rapid identification of fusobacterium nucleatum and fusobacterium necrophorum by fluorescence in situ hybridization. | identification of clinically relevant fusobacterium spp. is hampered by their slow growth, their frequent occurrence in polymicrobial culture, and the low reliability of biochemical differentiation methods. a newly developed fluorescence in situ hybridization (fish) assay allowed reliable and rapid identification of fusobacterium necrophorum and fusobacterium nucleatum from culture. preliminary results show that the method offers the perspective for direct detection of these pathogens in blood c ... | 2007 | 17350209 |
| differential activation of nf-kappab and gene expression in oral epithelial cells by periodontal pathogens. | to investigate the molecular effects of the periodontopathogens fusobacterium nucleatum (fn) and porphyromonas gingivalis (pg) on the oral epithelium, the h400 oral epithelial cell line was cultured in the presence of non-viable bacteria. following confirmation of the presence of transcripts for the bacterial pattern recognition receptors in h400 cells, toll-like receptors -2, -4 and -9, and components of the nf-kappab signalling pathway, immunocytochemical analyses were performed showing that n ... | 2007 | 17355248 |
| signaling cascades triggered by bacterial metabolic end products during reactivation of kaposi's sarcoma-associated herpesvirus. | the present studies explore the role of polymicrobial infection in the reactivation of kaposi's sarcoma-associated herpesvirus (kshv) and analyze signaling pathways activated upon this induction. we hypothesized that activation of the cellular stress-activated mitogen-activated protein kinase (mapk) p38 pathway would play a key role in the bacterium-mediated disruption of viral latency similar to that of previously reported results obtained with other inducers of gammaherpesvirus lytic replicati ... | 2007 | 17376930 |
| evidence of periopathogenic microorganisms in placentas of women with preeclampsia. | periodontal disease is a chronic inflammatory infectious disease that may act as a focus of infection. preeclampsia is a pregnancy-specific hypertensive disorder that often leads to maternal morbidity and mortality. acute atherosis, the placental lesion of preeclampsia, shares many histopathological features with atherosclerosis. recently, chronic infection was linked to the initiation of atherosclerosis. oral pathogens have been detected in atherosclerotic plaques, where they may play a role in ... | 2007 | 17397314 |
| case report: lemierre syndrome presenting with fever and pharyngitis. | 2007 | 17429891 | |
| the in vivo dynamics of streptococcus spp., actinomyces naeslundii, fusobacterium nucleatum and veillonella spp. in dental plaque biofilm as analysed by five-colour multiplex fluorescence in situ hybridization. | the formation and composition of dental plaque biofilm in vivo are important factors which influence the development of gingivitis, caries and periodontitis. studying dental plaque biofilm in in vitro models can cause an oversimplification of the real conditions in the oral cavity. in this study, bovine enamel slabs were fixed in an individual acrylic appliance in situ to quantify dental plaque formation and composition using multiplex fluorescence in situ hybridization (fish) and confocal laser ... | 2007 | 17446294 |
| sonoporation is an efficient tool for intracellular fluorescent dextran delivery and one-step double-crossover mutant construction in fusobacterium nucleatum. | studies of microorganisms are often hindered by a lack of effective genetic tools. one such example is fusobacterium nucleatum, a gram-negative anaerobe associated with various human infections, including those causing periodontal disease and preterm birth. the first double-crossover allelic-exchange mutant in f. nucleatum was recently constructed using sonoporation, a novel ultrasound-mediated intracellular delivery method, demonstrating potential for bacterial gene transfection. to better unve ... | 2007 | 17449701 |
| comparison of curet and paper point sampling of subgingival bacteria as analyzed by real-time polymerase chain reaction. | the outcome of microbiological diagnostics may depend on the sampling technique. it was the aim of the present study to compare two widely used sampling techniques for subgingival bacteria using quantitative real-time polymerase chain reaction. | 2007 | 17470026 |
| in vitro antimicrobial activity of propolis samples from different geographical origins against certain oral pathogens. | propolis is an agent having antimicrobial properties, however, its composition can vary depending on the area where it is collected. in the present study, the antimicrobial activity of five propolis samples, collected from four different regions in turkey and from brazil, against nine anaerobic strains was evaluated. ethanol extracts of propolis (eep) were prepared from propolis samples and we determined minimum inhibitory concentrations (mic) and minimum bactericidal concentrations (mbc) of eep ... | 2007 | 17475517 |
| activation of toll-like receptors 2 and 4 by gram-negative periodontal bacteria. | periodontitis is a chronic infectious disease associated with a gram-negative subgingival microflora. bacterial components stimulate, among other receptors, toll-like receptor (tlr) 2 and/or tlr4. accumulating evidence indicates that both qualitatively and quantitatively distinct immune responses result from the triggering of tlr2 as compared to tlr4 triggering. the aim was to study the interaction of porphyromonas gingivalis, actinobacillus actinomycetemcomitans, tannerella forsythensis, prevot ... | 2007 | 17488439 |
| infection at titanium implants with or without a clinical diagnosis of inflammation. | to assess the microbiota at implants diagnosed with peri-implantitis, implant mucositis, or being clinically healthy. | 2007 | 17517058 |
| periodontal bacterial dna suppresses the immune response to mutans streptococcal glucosyltransferase. | certain cpg motifs found in bacterial dna enhance immune responses through toll-like receptor 9 (tlr-9) and may also demonstrate adjuvant properties. our objective was to determine if dna from bacteria associated with periodontal disease could affect the immune response to other bacterial antigens in the oral cavity. streptococcus sobrinus glucosyltransferase (gtf), an enzyme involved in dental caries pathogenesis, was used as a test antigen. rowett rats were injected with aluminum hydroxide (al ... | 2007 | 17517867 |
| odontogenic bacteria in periodontal disease and resistance patterns to common antibiotics used as treatment and prophylaxis in odontology in spain. | resistance in streptococci or gram-negative bacteria is associated with antibiotic consumption. scarce information exists on the antibiotic susceptibility of bacterial isolates from patients with periodontitis in countries with high antibiotic consumption, as this is an area in which microbiological testing is not performed in daily practice. the present study was undertaken to explore the susceptibility of bacterial isolates in periodontitis to antibiotics prescribed in odontology in spain as t ... | 2007 | 17530037 |
| effect of an essential oil-containing antimicrobial mouthrinse on specific plaque bacteria in vivo. | this study was conducted to investigate the effect of rinsing with an essential oil-containing mouthrinse on levels of specific supra and subgingival bacteria in subjects with gingivitis. | 2007 | 17535287 |
| bacterial species in subgingival plaque and oral bone loss in postmenopausal women. | oral bacteria are widely recognized in the etiology of periodontal disease. we investigated the prevalence of subgingival bacterial infection with eight species, tested associations between infection and oral bone loss, and assessed potential confounding factors and effect modifiers of those associations in a large community-based cohort of postmenopausal women. | 2007 | 17539719 |
| co-adhesion and biofilm formation by fusobacterium nucleatum in response to growth ph. | fusobacterium nucleatum is a gram-negative anaerobic organism considered to play an important role in the progression of periodontal disease and is commonly found in clinical infections of other body sites. apart from its metabolic versatility, its cell-surface properties enable it to attach to epithelial cells, collagen, gingival epithelial cells and other bacterial genera, but not with other fusobacteria. the development of periodontitis is associated with a rise in ph in the gingival sulcus t ... | 2007 | 17540586 |
| a novel tem contrasting technique for extracellular polysaccharides in in vitro biofilms. | in the past, the visualization of the extracellular matrix of biofilms on an ultrastructural level was hampered by shrinkage artifacts. in addition, the reproducible contrasting of extracellular polysaccharides (eps) has not satisfactorily been solved. here we describe a method overcoming these difficulties, which produces artifact-free transmission electron microscopic (tem) images using multispecies biofilms grown in vitro. sufficient contrast was achieved by replacing schiff's reagent with th ... | 2007 | 17557287 |
| activation of protective responses in oral epithelial cells by fusobacterium nucleatum and human beta-defensin-2. | oral epithelia are constantly exposed to non-pathogenic (commensal) bacteria, but generally remain healthy and uninflamed. fusobacterium nucleatum, an oral commensal bacterium, strongly induces human beta-defensin-2 (hbd2), an antimicrobial and immunomodulatory peptide, in gingival epithelial cells (gecs). hbd2 is also expressed in normal oral tissue leading to the hypothesis that oral epithelia are in an activated state with respect to innate immune responses under normal in vivo conditions. in ... | 2007 | 17577065 |
| physiological alterations of a fusobacterium nucleatum strain exposed to oxidative stress. | the purpose of this study was to investigate the effect of oxidative stress on physiological and genetic characteristics of fusobacterium nucleatum and its interference on this microbial identification methods. | 2007 | 17584449 |
| production of antagonistic substance by eikenella corrodens isolated from the oral cavity of human beings with and without periodontal disease. | antagonistic abilities may confer ecological advantages for micro-organisms in competitive ecosystems. however, reports regarding this phenomenon in eikenella corrodens are not available. | 2007 | 17584471 |
| fada from fusobacterium nucleatum utilizes both secreted and nonsecreted forms for functional oligomerization for attachment and invasion of host cells. | fusobacterium nucleatum is a gram-negative anaerobe associated with various human infections, including periodontal diseases and preterm birth. a novel fada adhesin was recently identified for host-cell binding. it consists of 129 amino acid residues, with an 18-amino acid signal peptide. expression of fada in escherichia coli enhanced bacterial binding to host epithelial and endothelial cells. in both e. coli and f. nucleatum, fada exists in two forms, the intact pre-fada and the secreted matur ... | 2007 | 17588948 |
| association of a high-molecular weight arginine-binding protein of fusobacterium nucleatum atcc 10953 with adhesion to secretory immunoglobulin a and coaggregation with streptococcus cristatus. | fusobacterium nucleatum coaggregates with a diverse range of bacterial species, and binds to host tissues and proteins such as immunoglobulin. these interactions may support the attachment of a variety of organisms to oral surfaces and can facilitate the invasion of soft tissues. we hypothesized that coaggregation with streptococci and immunoglobulin binding may occur by a common adhesin sensitive to l-arginine. | 2007 | 17600532 |
| cultivable bacteria in infected root canals as identified by 16s rrna gene sequencing. | introduction: traditionally, cultivable bacteria isolated from infected root canals have been identified by phenotype-based methods. because 16s ribosomal rna (rrna) gene sequencing has emerged as a more accurate and reliable tool for bacterial identification, the present study applied this approach to identify bacterial isolates recovered from the root canals of teeth with chronic apical periodontitis. methods: anaerobic techniques were used for culturing; identification of the isolates was car ... | 2007 | 17600539 |
| femoral vein thrombophlebitis and septic pulmonary embolism due to a mixed anaerobic infection including solobacterium moorei: a case report. | abstract: | 2007 | 17605768 |
| the effect of mineral trioxide aggregate on phagocytic activity and production of reactive oxygen, nitrogen species and arginase activity by m1 and m2 macrophages. | to assess the influence of co-culture with mineral trioxide aggregate (mta) on phagocytosis and the production of reactive oxygen intermediates (roi) and nitrogen (no) species and the arginase activity by m1 and m2 peritoneal macrophages. | 2007 | 17627697 |
| development of a genus-specific pcr assay for the molecular detection, confirmation and identification of fusobacterium spp. | a genus-specific polymerase chain reaction (pcr)-based assay is developed for the detection and identification of clinically relevant fusobacterium species, including f. nucleatum and f. necrophorum. two 16s ribosomal dna (rdna) primers, fuso1 (forward primer: 5'-gag aga gct ttg cgt cc-3' [17-mer]) and fuso 2 (reverse primer: 5'-tgg gcg ctg agg ttc gac -3' [18-mer]) are designed to target conserved regions of the 16s rdna gene for fusobacterium spp. subsequent proof-of-principle studies employin ... | 2007 | 17633142 |
| [lemierre syndrome]. | 2007 | 17633598 | |
| role of anaerobes in acute pelvic inflammatory disease. | pouch of douglas aspirates were collected from 50 women with history and examination suggestive of acute pelvic inflammatory disease (pid) and 20 healthy women admitted for tubal ligation served as control. a total of 57 microorganisms were isolated from 37 patients out of 50 in study group. of 37 positive cultures 21(56.7%) were monomicrobial and 16(43.2%) were polymicrobial. most common symptom in study group was lower abdominal pain (90%), vaginal discharge (70%) and irregular bleeding (40%) ... | 2003 | 17643017 |
| targeted profiling of oral bacteria in human saliva and in vitro biofilms with quantitative real-time pcr. | an in vitro plaque model based on the use of human salivary bacteria and tooth-like surfaces was previously developed for studying the formation of oral biofilm and its use for pre-clinical testing of candidate antimicrobial or antiplaque agents. in this study, a quantitative taqman pcr assay (qpcr) was developed to compare the bacterial compositions of in vitro biofilms to parent saliva samples, and to determine the relative contributions of different species in the formation of the oral biofil ... | 2007 | 17653931 |
| genome sequence of fusobacterium nucleatum subspecies polymorphum - a genetically tractable fusobacterium. | fusobacterium nucleatum is a prominent member of the oral microbiota and is a common cause of human infection. f. nucleatum includes five subspecies: polymorphum, nucleatum, vincentii, fusiforme, and animalis. f. nucleatum subsp. polymorphum atcc 10953 has been well characterized phenotypically and, in contrast to previously sequenced strains, is amenable to gene transfer. we sequenced and annotated the 2,429,698 bp genome of f. nucleatum subsp. polymorphum atcc 10953. plasmid pfn3 from the stra ... | 2007 | 17668047 |
| effects of glass ionomer and microfilled composite subgingival restorations on periodontal tissue and subgingival biofilm: a 6-month evaluation. | this 6-month study evaluated the effects of resin-modified glass-ionomer cement (rmgi) and microfilled composite (mc) subgingival restorations on periodontal tissues and subgingival biofilm. | 2007 | 17668971 |
| fusobacterium nucleatum induces fetal death in mice via stimulation of tlr4-mediated placental inflammatory response. | intrauterine infection plays a pivotal role in preterm birth (ptb) and is characterized by inflammation. currently, there is no effective therapy available to treat or prevent bacterial-induced ptb. using fusobacterium nucleatum, a gram-negative anaerobe frequently associated with ptb, as a model organism, the mechanism of intrauterine infection was investigated. previously, it was shown that f. nucleatum induced preterm and term stillbirth in mice. fusobacterial-induced placental infection was ... | 2007 | 17675512 |
| hemoglobin and lps act in synergy to amplify the inflammatory response. | vascular disruption and bleeding during periodontitis likely increase the levels of hemoglobin in gingival crevicular fluid. the aim of this study was to investigate the effect of hemoglobin on the inflammatory responses of human macrophages stimulated with lipopolysaccharides (lps) isolated from periodontopathogens. the production of interleukin-1 beta (il-1beta), il-6, il-8, and tumor necrosis factor alpha (tnf-alpha) by macrophages following challenges with porphyromonas gingivalis and fusoba ... | 2007 | 17720859 |
| the impact of a low-frequency chlorhexidine rinsing schedule on the subgingival microbiota (the teeth clinical trial). | information on the efficacy of chlorhexidine (chx) rinsing on the subgingival microbiota is limited. this study tested if intermittent chx rinsing over 5 years had an impact on the subgingival microbiota. | 2007 | 17760545 |
| functionalization of pvdf membranes with carbohydrate derivates for the controlled delivery of chlorhexidin. | maltodextrin (mx) was fixed onto pvdf membranes in order to create a drug delivery guided tissue regeneration (gtr) device with controlled drug delivery properties. pvdf microporous membranes were treated by a mixture of mx and citric acid, resulting to an 18 wt% increase of the supports. mx grafted membrane could capture 103 mg/g chlorhexidin digluconate (digchx) instead of 1mg/g for a virgin membrane. a neutralization step was performed before the biological tests. viability tests confirmed th ... | 2007 | 17804290 |
| comparison of growth and susceptibility to sodium hypochlorite of mono- and dual-species biofilms of fusobacterium nucleatum and peptostreptococcus (micromonas) micros. | this study compared growth and susceptibility to different concentrations of sodium hypochlorite (naocl) of mono- and dual-species biofilms of fusobacterium nucleatum or peptostreptococcus micros in vitro at 24 hours or 96 hours. a mann-whitney u test revealed that although at 24 hours dual-species biofilms had similar viable counts to those of monospecies biofilms (p > 0.001), they were more resistant to naocl (p < 0.001). at 96 hours, both microorganisms had higher viable counts and were more ... | 2007 | 17804319 |
| [application of "microring an" to "level 1": presumptive identification of anaerobic gram-negative bacilli]. | commonly isolated anaerobic gram-negative rods (4 genus 64 strains), some other important gram-negative anaerobic species (9 genus 45 strains), and cigar-shaped clostridia (11 strains) were studied on their susceptibility patterns to 6 agents on "microring an". some modifications were made in the methods and interpretation of results. susceptibility patterns to erythromycin, rifampicin, colistin, benzylpenicillin, kanamycin, and vancomycin were following (sensitive [s], intermediate [i], resista ... | 2006 | 17822332 |
| antimicrobial effect of triclosan and triclosan with gantrez on five common endodontic pathogens. | microbial control of the root canal system is one of the key objectives of root canal therapy. triclosan is a widely accepted broad spectrum antimicrobial agent proven to be effective against many gram-positive and gram-negative bacteria. triclosan acts by blocking bacterial fatty acid biosynthesis. the addition of gantrez copolymer has been shown to enhance the antimicrobial activity of triclosan. the purpose of this study was to determine the minimum inhibitory and bactericidal concentrations ... | 2007 | 17889698 |
| implant surface analysis and microbiologic evaluation of failed implants retrieved from smokers. | the aim of this study was to evaluate the microbiota and surface of failed titanium dental implants from 4 manufacturers. twelve mobile dental implants were retrieved from 10 smokers after 3 to 10 years of functional loading. before implant removal, microbial samples were taken and evaluated using polymerase chain reaction. after implant removal, analyses of the failed implant surfaces were performed using scanning electron microscopy and energy-dispersive spectrometer x-ray. periodontal pathoge ... | 2007 | 17912965 |
| the trimeric periplasmic chaperone skp of escherichia coli forms 1:1 complexes with outer membrane proteins via hydrophobic and electrostatic interactions. | the interactions of outer membrane proteins (omps) with the periplasmic chaperone skp from escherichia coli are not well understood. we have examined the binding of skp to various omps of different origin, size, and function. these were ompa, ompg, and yaet (omp85) from escherichia coli, the translocator domain of the autotransporter nalp from neisseria meningitides, foma from fusobacterium nucleatum, and the voltage-dependent anion-selective channel, human isoform 1 (hvdac1) from mitochondria. ... | 2007 | 17928002 |
| bacterial leakage along the implant-abutment interface of premachined or cast components. | in recent clinical studies, contamination of the inner parts of dental implants through bacterial penetration along the implant components has been observed. the aim of the present in-vitro study was to investigate leakage of fusobacterium nucleatum through the interface between implants and premachined or cast abutments. both premachined (n=10) and cast (n=10) implant-abutment assemblies were inoculated with 3.0 microl of microbial inoculum. the assemblies were completely immersed in 5.0 ml of ... | 2008 | 17931833 |
| compressed mints and chewing gum containing magnolia bark extract are effective against bacteria responsible for oral malodor. | flavors and natural botanic extracts are often used in chewing gum and compressed mints for breath freshening and relief of oral malodor. the oral malodor is a result of bacterial putrification of proteinaceous materials from food or saliva. in this study, magnolia bark extract (mbe) and its two main components, magnolol and honokiol, were evaluated by the minimum inhibition concentration (mic) test. the inhibitory effect of mbe mint was further evaluated by a kill-time assay study. in addition, ... | 2007 | 17949053 |
| evaluation of the microbiota of primary endodontic infections using checkerboard dna-dna hybridization. | the aim of this study was to evaluate the composition of the microbiota of primary endodontic infections in 111 selected cases of single-rooted teeth with necrotic pulp. | 2007 | 17949342 |
| improved accuracy in terminal restriction fragment length polymorphism phylogenetic analysis using a novel internal size standard definition. | terminal restriction fragment length polymorphism (t-rflp) analysis is commonly used to analyze microbial communities, including oral microflora. however, accurate identification of terminal restriction fragment (t-rf) origins is prevented by unpredictable errors in sizing, thus necessitating the clone library analysis. to minimize sizing errors, we proposed optimizing the size definition of internal standards. | 2007 | 17949346 |
| antimicrobial profiles of prevotella spp. and fusobacterium nucleatum isolated from periodontal infections in a selected area of southern italy. | in view of the increasing antibiotic resistance of anaerobic gram-negative bacteria, we determined the antimicrobial profile of 55 periodontal anaerobic gram-negative bacteria correlated with human infections, comprising 16 strains of fusobacterium nucleatum and 39 strains of prevotella spp. isolated from periodontal pockets of 26 adults suffering from chronic periodontitis. all the strains of f. nucleatum were susceptible to amoxicillin/clavulanic acid, doxycycline, metronidazole, moxifloxacin ... | 2007 | 17954025 |
| innate immune responses of gingival epithelial cells to nonperiodontopathic and periodontopathic bacteria. | we have previously reported different susceptibilities of periodontopathic and nonperiodontopathic bacteria to antimicrobial peptides and phagocytosis by neutrophils. differences between the two groups of bacteria may exist also in their ability to induce immune responses from the host. therefore, we evaluated the effects of various oral bacteria on innate immune responses by gingival epithelial cells. | 2007 | 17956462 |
| inhibitory effect of cranberry polyphenol on biofilm formation and cysteine proteases of porphyromonas gingivalis. | the purpose of this study was to investigate the effects of cranberry polyphenol fraction on biofilm formation and activities of arg-gingipain and lys-gingipain in porphyromonas gingivalis. | 2007 | 17956474 |
| antimicrobial effect of propolis and other substances against selected endodontic pathogens. | the aim of this study was to determine the antimicrobial effect of ethanol extract of propolis (eep) and intracanal medicaments calcium hydroxide, camphorated paramonochlorophenol, and formocresol by means of the macrodilution method using the reinforced clostridial medium (rcm) and brucella and brain heart infusion media. | 2007 | 17964476 |
| involvement of phospholipase d in regulating expression of anti-microbial peptide human beta-defensin-2. | human beta-defensin expression correlates with differentiation in oral epithelium, and calcium ion, an important regulator of epithelial differentiation, plays a critical role in regulation of human beta-defensin-2 (hbd-2) mrna expression. phospholipase d (pld) also regulates epithelial differentiation. therefore, we examined the role of pld in hbd-2 up-regulation by cell wall extract of fusobacterium nucleatum and phorbol 12-myristate 13-acetate (pma), two known hbd-2 activators. we found that ... | 2008 | 17986621 |
| the inhibition of dentifrice containing the lotus leaf-derived inhibitor on periodontitis-related bacteria in vitro. | to search for a substance in lotus leaf that could efficiently be used in toothpaste for preventing periodontal disease. | 2007 | 17992914 |
| [the development and in vitro experiment study of a bio-type root canal filling sealer using calcium phosphate cement]. | the purpose of this study is to develop a novel root canal filling sealers based on calcium phosphate cement (cpc), and to evaluate its physical-chemical properties and in vitro antibacterial activity on the predominant bacteria infecting root canal. | 2007 | 18004487 |
| soluble cd14 discriminates slight structural differences between lipid as that lead to distinct host cell activation. | soluble cd14 (scd14) in serum is known to sensitize host cells to lps. in the present study, the contributions of scd14 and lps-binding protein to a lipid a moiety from lps preparations of periodontopathogenic fusobacterium nucleatum sp. nucleatum were compared with that of escherichia coli-type synthetic lipid a (compound 506). f. nucleatum lipid a was identified to be a hexa-acylated fatty acid composed of tetradecanoate (c(14)) and hexadecanoate (c(16)), similar to dodecanoate (c(12)) and c(1 ... | 2007 | 18025213 |
| effect of chlorhexidine/thymol and fluoride varnishes on dental biofilm formation in vitro. | this study evaluated the effect of chlorhexidine/thymol (chx/t) and fluoride (f) varnishes on biofilm formation in vitro. hydroxyapatite discs coated with varnish were first immersed in saline for 0, 3, 7 or 14 d, then immersed in pasteurized saliva. the discs were incubated for 20 h with a bacterial suspension containing actinomyces naeslundii, fusobacterium nucleatum, streptococcus oralis, and veillonella dispar. uncoated discs were used as controls. growth of bacteria on the discs was evaluat ... | 2007 | 18028054 |
| improved diagnosis specificity in bone and joint infections using molecular techniques. | the microbiological diagnosis of osteoarticular infections currently relies on microbiological cultures, to specifically target treatment. however, these conventional methods sometimes lack of sensitivity and of specificity to establish definitive diagnosis. this study was conducted to determine whether molecular method could improve bacterial bone and joint infection diagnosis. | 2007 | 18029022 |
| [case report: positive signals from automated blood-culture system with negative direct gram examination]. | detection of positive haemoculture is usually managed by an automated system. when a bottle is detected positive but that the gram coloration does not reveal germs by direct examination, transfer onto chocolate blood agar generally allows to confirm or infirm bacteraemia. in light of a case of fusobacterium nucleatum bacteraemia, we discuss the opportunity of pairing it with an enrichment broth. m. n, hospitalized in the hepatogastroenterology department, runs a fever of undetermined origin. thr ... | 2007 | 18031953 |
| effect of tissue fluids on hydrophobicity and adherence of enterococcus faecalis to dentin. | this in vitro study was carried out to determine (1) the hydrophobicity of selected oral bacteria, (2) the influence of growth media (saliva and serum) and mode of growth (planktonic or biofilm) on the hydrophobicity of enterococcus faecalis, and (3) the influence of growth media and conditioning fluids on the adherence of e. faecalis to dentin. the ability to bind to a hydrocarbon phase (xylene) was used as an index of relative hydrophobicity of cells. fluorescent microscopy-based technique was ... | 2007 | 18037050 |
| early soft tissue healing around one-stage dental implants: clinical and microbiologic parameters. | despite the potential significance of early soft tissue healing to long-term outcomes, this aspect of one-stage dental implants has not been investigated. the purpose of this prospective study was to characterize clinical and microbiologic parameters of early soft tissue healing around dental implants placed following a one-stage protocol. | 2007 | 18062110 |
| effect of rinsing with an essential oil-containing mouthrinse on subgingival periodontopathogens. | studies have shown that the nature and amount of supragingival plaque can influence the composition of the contiguous subgingival flora. the purpose of this study was to investigate the effect of rinsing with an essential oil antimicrobial mouthrinse on levels of representative subgingival bacteria in subjects with mild to moderate periodontitis. | 2007 | 18062115 |
| analysis of the antimicrobial activity of local anaesthetics used for dental analgesia. | seven local anaesthetics and their active anaesthetic components [ultracaine d-s (articaine hydrochloride), carbostesin (bupivacaine hydrochloride), scandicaine (mepivacaine hydrochloride), xylonest (prilocaine hydrochloride), xylocaine (lidocaine hydrochloride), hostacaine (butanilicaine phosphate) and novocaine (procaine hydrochloride)] were tested for their antimicrobial activity against 311 bacterial strains from 52 different species and 14 candida albicans strains. the tested pathogens were ... | 2008 | 18065672 |
| antimicrobial activity of mahonia aquifolium and two of its alkaloids against oral bacteria. | extracts or alkaloids isolated from mahonia aquifolium exhibit antimicrobial activity against gram-positive and gram-negative bacteria, fungi, and protozoa. in this study the bacteriostatic and bacteriocidal activities of a m. aquifolium extract and two of its major alkaloids, berberine chloride and oxyacanthine sulphate, were tested in vitro against nine different oral bacteria. minimum inhibitory concentrations were in the range from < or = 0.0031% to 0.1993% for the m. aquifolium extract, fro ... | 2007 | 18072463 |
| effect of photo-activated disinfection on endodontic pathogens ex vivo. | to test the hypothesis that photo-activated disinfection (pad) has a bactericidal effect on pathogens inoculated in root canals, with emphasis on biofilm formation/destruction. | 2008 | 18081808 |
| quantitative analysis of association between herpesviruses and bacterial pathogens in periodontitis. | the development of human periodontitis may depend upon cooperative interactions among herpesviruses, specific pathogenic bacteria and tissue-destructive inflammatory mediators. this study sought to identify associations among human cytomegalovirus, epstein-barr virus and six putative periodontopathic bacteria in periodontitis lesions. | 2008 | 18086168 |
| stimulation of fusobacterium nucleatum biofilm formation by porphyromonas gingivalis. | bacterial infection is a major cause of periapical periodontitis. eradication of these microorganisms from apical lesions is essential to the success of endodontic treatment. the aim of this study was to clarify the molecular interaction between fusobacterium nucleatum, porphyromonas gingivalis and other microorganisms associated with periapical periodontitis. | 2008 | 18173791 |
| proteomic analysis of ampicillin-resistant oral fusobacterium nucleatum. | fusobacterium nucleatum represents one of the predominant anaerobic species in the oral microbiota. penicillin-resistant f. nucleatum have been isolated from intra- and extraoral infections. this study aimed to assess ampicillin resistance in f. nucleatum by investigating the synthesis of resistance-associated proteins. | 2008 | 18173796 |
| relationship between campylobacter rectus and periodontal status during pregnancy. | in a previous study, we showed that the growth of campylobacter rectus is stimulated by the presence of female sex hormones in the culture medium. in the present study, we examined the relationship between c. rectus levels in the saliva and the periodontal status of pregnant women. | 2008 | 18173799 |
| microflora in oral ecosystems in subjects with radiation-induced hyposalivation. | to analyse the microbial flora in specific oral sites in 13 dentate subjects, 6-8 months after completed radiation therapy (rt group) and in 13 matched controls. | 2008 | 18208474 |
| genetic and biochemical characterization of oxa-63, a new class d beta-lactamase from brachyspira pilosicoli bm4442. | brachyspira pilosicoli bm4442, isolated from the feces of a patient with diarrhea at the hospital saint-michel in paris, was resistant to oxacillin (mic > 256 microg/ml) but remained susceptible to cephalosporins and to the combination of amoxicillin and clavulanic acid. cloning and sequencing of the corresponding resistance determinant revealed a coding sequence of 807 bp encoding a new class d beta-lactamase named oxa-63. the bla oxa-63 gene was chromosomally located and not part of a transpos ... | 2008 | 18212108 |
| in vitro antioxidant potential of medicinal plant extracts and their activities against oral bacteria based on brazilian folk medicine. | this study aims to determine antibacterial activities of cocos nucifera (husk fiber), ziziphus joazeiro (inner bark), caesalpinia pyramidalis (leaves), aqueous extracts and aristolochia cymbifera (rhizomes) alcoholic extract against prevotella intermedia, porphyromonas gingivalis, fusobacterium nucleatum, streptococcus mutans and lactobacillus casei. the antioxidant activity and acute toxicity of these extracts were also evaluated. | 2008 | 18243157 |
| a case report of a pyogenic liver abscess caused by fusobacterium nucleatum in a patient with autosomal dominant polycystic kidney disease undergoing hemodialysis. | pyogenic liver abscess (pla) is a process with significant morbidity and mortality and is a rare complication in an aisled way in patients with autosomal dominant polycystic kidney disease (adpkd). in addition to hepatic cyst infection, intracystic hemorrhage is another complication seen in adpkd patients; however, the liver parenchyma itself remains normal. a pla located in normal liver tissue in these kinds of patients has not been previously reported. fusobacterium nucleatum is an anaerobic b ... | 2008 | 18257820 |
| smap29 congeners demonstrate activity against oral bacteria and reduced toxicity against oral keratinocytes. | cathelicidins are antimicrobial peptides found in epithelial and mucosal tissues as well as the secondary granules of neutrophils. smap29, a sheep cathelicidin, has differential antimicrobial properties against various pathogens, including periodontal organisms. the purpose of this study was to evaluate the antimicrobial properties and cytotoxicity of smap29, smap28, and three congeners (smap18a, smap18d, and smap14a). | 2008 | 18279175 |
| aggregatibacter actinomycetemcomitans serotype f o-polysaccharide mediates coaggregation with fusobacterium nucleatum. | intergeneric bacterial coaggregation may play an important role in plaque development. | 2008 | 18279180 |
| colonization pattern of periodontal bacteria in japanese children and their mothers. | the purpose of this study was to determine the time of infection by anaerobic gram-negative rods associated with periodontal disease, and to clarify their transmission from mother to child. | 2008 | 18302616 |
| interactions between salivary bifidobacterium adolescentis and other oral bacteria: in vitro coaggregation and coadhesion assays. | coaggregation assays were performed to investigate interactions between oral bifidobacterium adolescentis and other oral bacterial species. bifidobacterium adolescentis olb6410 isolated from the saliva of healthy humans did not coaggregate with actinomyces naeslundii jcm8350, streptococcus mitis ols3293, streptococcus sanguinis jcm5708, veillonella parvula atcc17745 or porphyromonas gingivalis ob7124, but it did coaggregate with fusobacterium nucleatum jcm8532. subsequent examination of biofilm ... | 2008 | 18312575 |