Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| effects of intertidal harvest practices on levels of vibrio parahaemolyticus and vibrio vulnificus bacteria in oysters. | vibrio parahaemolyticus and vibrio vulnificus can grow rapidly in shellfish subjected to ambient air conditions, such as during intertidal exposure. in this study, levels of total and pathogenic (tdh(+) and/or trh(+)) v. parahaemolyticus and total v. vulnificus were determined in oysters collected from two study locations where intertidal harvest practices are common. samples were collected directly off intertidal flats, after exposure (ambient air [washington state] or refrigerated [new jersey] ... | 2016 | 27208133 |
| glucose induces delocalization of a flagellar biosynthesis protein from the flagellated pole. | to survive in a continuously changing environment, bacteria sense concentration gradients of attractants or repellents, and purposefully migrate until a more favourable habitat is encountered. while glucose is known as the most effective attractant, the flagellar biosynthesis and hence chemotactic motility has been known to be repressed by glucose in some bacteria. to date, the only known regulatory mechanism of the repression of flagellar synthesis by glucose is via downregulation of the camp l ... | 2016 | 27218601 |
| prevalence and distribution of vibrio spp. in wild aquatic birds of the southern caribbean sea, venezuela, 2011-12. | vibrio spp. are associated with waterbirds mainly in temperate latitudes. we evaluated the prevalence and distribution of vibrio spp. from fecal samples of resident and migratory aquatic birds collected during october 2011 and march 2012 at two coastal sites in the tropical southern caribbean sea. we amplified dna by pcr in 40% of samples, resulting in 47% and 36% estimated prevalence for resident and migratory birds in cuare wildlife refuge, and 33% and 44% in margarita island, respectively. we ... | 2016 | 27224211 |
| vibrio vulnificus vvha induces autophagy-related cell death through the lipid raft-dependent c-src/nox signaling pathway. | vvha, a virulent factor of vibrio (v.) vulnificus, induces acute cell death in a destructive manner. autophagy plays an important role in cell death, but the functional role of vvha in autophagy-related cell death has not been elucidated yet. we found that rvvha significantly increased lc3 puncta formation and autophagic flux in promoting the cell death of human intestinal epithelial caco-2 cells. the cell death induced by rvvha was independent of lysosomal permeabilizaton and caspase activation ... | 2016 | 27250250 |
| in vitro and in vivo antibacterial activity of tigecycline against vibrio vulnificus. | the aim of this study is to investigate the role of tigecycline in vibrio vulnificus infection. | 2016 | 27260781 |
| oxyr2 functions as a three-state redox switch to tightly regulate production of prx2, a peroxiredoxin of vibrio vulnificus. | the bacterial transcriptional regulator oxyr is known to function as a two-state redox switch. oxyr senses cellular levels of h2o2 via a "sensing cysteine" that switches from the reduced to a disulfide state upon h2o2 exposure, inducing the expression of antioxidant genes. the reduced and disulfide states of oxyr, respectively, bind to extended and compact regions of dna, where the reduced state blocks and the oxidized state allows transcription and further induces target gene expression by inte ... | 2016 | 27268058 |
| [postoperative necrotizing fasciitis: a rare and fatal complication]. | postoperative parietal complications can be exceptionally severe and serious threatening vital prognosis. necrotizing fasciitis is a rare infection of the skin and deep subcutaneous tissues, spreading along fascia and adipose tissue. it is mainly caused by group a streptococcus (streptococcus pyogenes) but also by other bacteria such as vibrio vulnificus, clostridium perfringens or bacteroides fragilis. necrotizing fasciitis is a real surgical and medical emergency. we report, in this study, a v ... | 2016 | 27279950 |
| deacylated lipopolysaccharides inhibit biofilm formation by gram-negative bacteria. | the extracellular polysaccharides of vibrio vulnificus play different roles during biofilm development. among them, the effect of lipopolysaccharide (lps), which is crucial for bacterial adherence to surfaces during the initial stage of biofilm formation, on the formation process was examined using various types of lps extracts. exogenously added lps strongly inhibited biofilm formation in a dose-dependent manner. in addition, the exogenous addition of a deacylated form of lps (dlps) also inhibi ... | 2016 | 27294580 |
| complete genome sequence of vibrio vulnificus forc_017 isolated from a patient with a hemorrhagic rash after consuming raw dotted gizzard shad. | vibrio vulnificus, a resident in the human gut, is frequently found in seafood, causing food-borne illnesses including gastroenteritis and severe septicemia. while v. vulnificus has been known to be one of the major food-borne pathogens, pathogenicity and virulence factors are not fully understood yet. to extend our understanding of the pathogenesis of v. vulnificus at the genomic level, the genome of v. vulnificus forc_017 isolated from a female patient experiencing a hemorrhagic rash was compl ... | 2016 | 27325916 |
| iron and fur in the life cycle of the zoonotic pathogen vibrio vulnificus. | in this study, we aimed to analyze the global response to iron in the broad-range host pathogen vibrio vulnificus under the hypothesis that iron is one of the main signals triggering survival mechanisms both inside and outside its hosts. to this end, we selected a strain from the main zoonotic clonal-complex, obtained a mutant in the ferric-uptake-regulator (fur), and analyzed their transcriptomic profiles in both iron-excess and iron-poor conditions by using a strain-specific microarray platfor ... | 2016 | 27348505 |
| induced disruption of the iron-regulatory hormone hepcidin inhibits acute inflammatory hypoferraemia. | withdrawal of iron from serum (hypoferraemia) is a conserved innate immune antimicrobial strategy that can withhold this critical nutrient from invading pathogens, impairing their growth. hepcidin (hamp1) is the master regulator of iron and its expression is induced by inflammation. mice lacking hamp1 from birth rapidly accumulate iron and are susceptible to infection by blood-dwelling siderophilic bacteria such as vibrio vulnificus. in order to study the innate immune role of hepcidin against a ... | 2016 | 27423740 |
| in vitro and in vivo anti-vibrio vulnificus activity of psammaplin a, a natural marine compound. | vibrio vulnificus is known to induce severely fulminant and fatal septicemia in susceptible hosts. in the present study, the antimicrobial activity of natural marine product-derived compounds against v. vulnificus, were investigated in vitro and in vivo. twelve pure compounds were isolated from natural marine products and their inhibitory effects on v. vulnificus-induced cytotoxicity were determined in int‑407 cells. among the 12 pure compounds tested, treatment with psammaplin a significantly s ... | 2016 | 27431807 |
| vibrio parahaemolyticus and vibrio vulnificus in south america: water, seafood and human infections. | the bacterial species, vibrio parahaemolyticus and vibrio vulnificus, are ubiquitous in estuaries and coastal waters throughout the world, but they also happen to be important human pathogens. they are concentrated by filter-feeding shellfish which are often consumed raw or undercooked, providing an important potential route of entry for an infective dose of these bacteria. vibrio parahaemolyticus can cause abdominal cramping, nausea, diarrhoea, vomiting, chills and fever. vibrio vulnificus can ... | 2016 | 27459915 |
| development of a quadruplex loop-mediated isothermal amplification assay for field detection of four vibrio species associated with fish disease. | a quadruplex loop-mediated isothermal amplification (lamp) method was developed to detect four vibrio species, including vibrio ichthyoenteri, vibrio parahaemolyticus, vibrio scophthalmi, and vibrio vulnificus, simultaneously. four sets of species-specific primers were designed with different restriction sites contained in the inner primers. the quadruplex lamp method could distinguish four vibrio species via the subsequent restriction enzyme analysis. the sensitivity of the quadruplex lamp meth ... | 2016 | 27468405 |
| characterization of clinical and environmental isolates of vibrio cidicii sp. nov., a close relative of vibrio navarrensis. | four vibrio spp. isolates from the historical culture collection at the centers for disease control and prevention, obtained from human blood specimens (n=3) and river water (n=1), show characteristics distinct from those of isolates of the most closely related species, vibrio navarrensis and vibrio vulnificus, based on phenotypic and genotypic tests. they are specifically adapted to survival in both freshwater and seawater, being able to grow in rich media without added salts as well as salinit ... | 2016 | 27468862 |
| role of extracellular matrix protein caba in resistance of vibrio vulnificus biofilms to decontamination strategies. | biofilms are recalcitrant and raise safety problems in the food industry. in this study, the role of caba, an extracellular matrix protein, in the resistance of the biofilms of vibrio vulnificus, a foodborne pathogen, to decontamination strategies was investigated. biofilms of the caba mutant revealed reduced resistance to detachment by vibration and disinfection by sodium hypochlorite compared to the biofilms of the parental wild type in vitro. the reduced resistance of the caba mutant biofilms ... | 2016 | 27485973 |
| simultaneous identification of 13 foodborne pathogens by using capillary electrophoresis-single strand conformation polymorphism coupled with multiplex ligation-dependent probe amplification and its application in foods. | capillary electrophoresis-single strand conformation polymorphism (ce-sscp) coupled with stuffer-free multiplex ligation-dependent probe amplification (mlpa) was developed to identify 13 species of foodborne pathogens simultaneously. species-specific mlpa probes were designed for nine of these species. these probes were targeted to the groel, glya, mms, tuf, inv, ipah, nuc, vvh, and 16s rrna genes, which corresponded to bacillus cereus, campylobacter coli, cronobacter sakazakii, enterococcus spp ... | 2016 | 27494415 |
| a rare glimpse into the morbid world of necrotising fasciitis: flesh-eating bacteria vibrio vulnificus. | necrotising fasciitis is one of the fatal skin and soft tissue infections. vibrio vulnificus is a rare cause of necrotising fasciitis; however, the disease is one of the major manifestations of the bacteria. here, we report one such case in a middle-aged male patient. he presented with the signs of bilateral lower limb cellulitis and altered sensorium. v. vulnificus was isolated from blood culture and also from debrided tissue. though the organism is well characterised, it is a rare causative ag ... | 2017 | 27514968 |
| faraday cage-type electrochemiluminescence immunosensor for ultrasensitive detection of vibrio vulnificus based on multi-functionalized graphene oxide. | a novel faraday cage-type electrochemiluminescence (ecl) immunosensor devoted to the detection of vibrio vulnificus (vv) was fabricated. the sensing strategy was presented by a unique faraday cage-type immunocomplex based on immunomagnetic beads (imbs) and multi-functionalized graphene oxide (go) labeled with (2,2'-bipyridine)(5-aminophenanthroline)ruthenium (ru-nh2). the multi-functionalized go could sit on the electrode surface directly due to the large surface area, abundant functional groups ... | 2016 | 27565793 |
| a c-type lectin that inhibits bacterial infection and facilitates viral invasion in black rockfish, sebastes schlegelii. | c-type lectins (ctls) are important pattern recognition receptors (prrs) that play vital roles in innate immunity. in teleosts, a number of ctls have been reported, but their in vivo effects on host defense are still limited. in this study, a ctl homolog (sslec1) was identified from black rockfish, sebastes schlegelii, and its structure, expression and biological function was analyzed. the open reading frame of sslec1 is 633 bp, with a 5'- untranslated region (utr) of 36 bp and a 3'- utr of 117 ... | 2016 | 27569982 |
| rt-pcr-dgge analysis to elucidate the dominant bacterial species of industrial spanish-style green table olive fermentations. | this paper describes the dominant bacterial species metabolically active through the industrial production of spanish-style manzanilla and gordal olives. for this purpose, samples (brines and fruits) obtained at 0, 15, and 90 fermentation days were analyzed by a culture-independent approach to determine viable cells by reverse transcription of rna and further pcr-dgge analysis, detecting at least 7 different species. vibrio vulnificus, lactobacillus plantarum group, and lactobacillus parafarragi ... | 2016 | 27582739 |
| rapidly developing and fatal vibrio vulnificus wound infection. | 2016 | 27617208 | |
| o-heterocyclic derivatives with antibacterial properties from marine bacterium bacillus subtilis associated with seaweed, sargassum myriocystum. | the brown seaweed, sargassum myriocystum associated with heterotrophic bacterium, bacillus subtilis mtcc 10407 (jf834075) exhibited broad-spectra of potent antibacterial activities against pathogenic bacteria aeromonas hydrophila, vibrio vulnificus, and vibrio parahaemolyticus. b. subtilis mtcc 10407 was found to be positive for polyketide synthetase (pks) gene, and therefore, was considered to characterize secondary metabolites bearing polyketide backbone. using bioassay-guided fractionation, t ... | 2017 | 27624095 |
| quantification of vibrio species in oysters from the gulf of mexico with two procedures based on mpn and pcr. | oysters can accumulate potentially pathogenic water bacteria. the objective of this study was to compare two procedures to quantify vibrio species present in oysters to determine the most sensitive method. we analyzed oyster samples from the gulf of mexico, commercialized in mexico city. the samples were inoculated in tubes with alkaline peptone water (apw), based on three tubes and four dilutions (10(-1) to 10(-4)). from these tubes, the first quantification of vibrio species was performed (mos ... | 2016 | 27709461 |
| raw ready-to-eat seafood safety: microbiological quality of the various seafood species available in fishery, hyper and online markets. | microbiological quality of 206 raw ready-to-eat seafood samples was investigated according to species (gizzard shad, halibut, rockfish, tuna, oyster and squid) and distribution channels (fishery, hyper and online market). enumeration of aerobic plate count and total coliforms (tc) and pathogenic bacteria (bacillus cereus, staphylococcus aureus and vibrio parahaemolyticus) was performed, and level of microbiological quality was classified into four groups: satisfactory, acceptable, unsatisfactory ... | 2017 | 27747902 |
| a silkworm infection model to investigate vibrio vulnificus virulence genes. | the halophilic marine bacterium, vibrio vulnificus, occasionally causes fatal septicemia in immunocompromised patients. mice are commonly used as experimental animals to investigate the virulence of v. vulnificus, however, a large number of mice are generally required for bioassays. the present study examined whether the invertebrate species, silkworms, can be used instead of mice to investigate v. vulnificus virulence. when the silkworms were inoculated with 1.2x107 colony forming units of v. v ... | 2016 | 27748924 |
| two new benzoate derivatives and one new phenylacetate derivative from a marine-derived fungus engyodontium album. | two new benzoate derivatives, ethyl 3,5-dimethoxy-2-propionylbenzoate (1) and ethyl 3,5-dihydroxy-2-propionylbenzoate (2), and one new phenylacetate derivative, ethyl 3,5-dimethoxy-2-propionylphenylacetate (3), together with 9 known compounds, were isolated from the fermentation of engyodontium album derived from deep sea sediment. their structures were elucidated by spectroscopic techniques, such as nmr, ir and hresims. compound 3 exhibited inhibitory activities against methicillin-resistant st ... | 2017 | 27750436 |
| differences in abundances of total vibrio spp., v. vulnificus, and v. parahaemolyticus in clams and oysters in north carolina. | filter feeding shellfish can concentrate pathogenic bacteria, including vibrio vulnificus and vibrio parahaemolyticus, as much as 100-fold from the overlying water. these shellfish, especially clams and oysters, are often consumed raw, providing a route of entry for concentrated doses of pathogenic bacteria into the human body. the numbers of foodborne infections with these microbes are increasing, and a better understanding of the conditions that might trigger elevated concentrations of these b ... | 2017 | 27793822 |
| vibrio vulnificus type 6 secretion system 1 contains anti-bacterial properties. | vibrio vulnificus is a bacterium responsible for severe gastroenteritis, sepsis and wound infections. gastroenteritis and sepsis are commonly associated with the consumption of raw oysters, whereas wound infection is often associated with the handling of contaminated fish. although classical virulence factors of this emerging pathogen are well characterised, there remains a paucity of knowledge regarding the general biology of this species. to investigate the presence of previously unreported vi ... | 2016 | 27798649 |
| occurrence of vibrio parahaemolyticus, vibrio cholerae and vibrio vulnificus in the clam ruditapes philippinarum (adams & reeve, 1850) from emilia romagna and sardinia, italy. | marine vibrios, vibrio parahaemolyticus, v. vulnificus and v. cholerae are responsible of the majority of food-borne human infections by consumption of bivalve shellfish. the aim of the present study was to ascertain the occurrence of these bacteria, and their potential pathogenicity, in the manila clam r. philippinarum from emilia romagna (er) and sardinia (sr) regions, italy. isolation was performed on chromagar(tm) vibrio with subculture on (thiosulfate-citrate-bile salts-sucrose) agar and m- ... | 2016 | 27800436 |
| season-specific occurrence of potentially pathogenic vibrio spp. on the southern coast of south korea. | vibrio species are widely distributed in warm estuarine and coastal environments, and they can infect humans through the consumption of raw and mishandled contaminated seafood. in this study, we aimed to isolate and observe the distribution of enteropathogenic vibrio spp. from environments of the southern coast of south korea over a season cycle. a total of 10,983 isolates of vibrio spp. were obtained from tidal water and mud samples over a 1-year period from five sampling sites along the southw ... | 2017 | 27836844 |
| a tfpi-1 peptide that induces degradation of bacterial nucleic acids, and inhibits bacterial and viral infection in half-smooth tongue sole, cynoglossus semilaevis. | tissue factor pathway inhibitor 1 (tfpi-1) is a serine protease inhibitor that inhibits tissue factor (tf)-mediated coagulation. the c-terminal region of tfpi-1 could be cleaved off and proved to be antimicrobial against a broad-spectrum of microorganism. in a previous study, a c-terminal peptide, tc24 (with 24 amino acids), derived from tongue sole (cynoglossus semilaevis) tfpi-1, was synthesized and found antibacterial against micrococcus luteus. in the present study, the antibacterial spectru ... | 2017 | 27840169 |
| non-cholera vibrios: the microbial barometer of climate change. | there is a growing interest in the role of climate change in driving the spread of waterborne infectious diseases, such as those caused by bacterial pathogens. one particular group of pathogenic bacteria - vibrios - are a globally important cause of diseases in humans and aquatic animals. these gram-negative bacteria, including the species vibrio vulnificus, vibrio parahaemolyticus and vibrio cholerae, grow in warm, low-salinity waters, and their abundance in the natural environment mirrors ambi ... | 2017 | 27843109 |
| pathogenic mechanisms of actin cross-linking toxins: peeling away the layers. | actin cross-linking toxins are produced by gram-negative bacteria from vibrio and aeromonas genera. the toxins were named actin cross-linking domains (acd), since the first and most of the subsequently discovered acds were found as effector domains in larger martx and vgrg toxins. among recognized human pathogens, acd is produced by vibrio cholerae, vibrio vulnificus, and aeromonas hydrophila. upon delivery to the cytoplasm of a host cell, acd covalently cross-links actin monomers into non-polym ... | 2017 | 27858184 |
| capsular polysaccharide production and serum survival of vibrio vulnificus are dependent on antitermination control by rfah. | the human pathogen vibrio vulnificus undergoes phase variation among colonial morphotypes, including a virulent opaque form which produces capsular polysaccharide (cps) and a translucent phenotype that produces little or no cps and is attenuated. here, we found that a v. vulnificus mutant defective for rfah antitermination control showed a diminished capacity to undergo phase variation and displayed significantly reduced distal gene expression within the group i cps operon. moreover, the rfah mu ... | 2016 | 27859050 |
| biogeochemical and hydrological drivers of the dynamics of vibrio species in two patagonian estuaries. | the ecology of the most relevant vibrio species for human health and their relation to water quality and biogeochemistry were studied in two estuaries in argentinian patagonia. vibrio cholerae and vibrio parahaemolyticus were reported in >29% of cases at the río colorado and río negro estuaries. neither the pandemic serogroups of vibrio cholerae o1, vibrio cholerae o139 nor the cholera toxin gene were detected in this study. however, several strains of v. cholerae (not o1 or o139) are able to ca ... | 2017 | 27871750 |
| visualizing evolutionary relationships of multidomain proteins: an example from receiver (rec) domains of sensor histidine kinases in the candidatus maribeggiatoa str. orange guaymas draft genome. | for multidomain proteins, evolutionary changes may occur at the domain as well as the whole-protein level. an example is presented here, with suggestions for how such complicated relationships might be visualized. earlier analysis of the candidatus maribeggiatoa str. orange guaymas (boguay; gammaproteobacteria) single-filament draft genome found evidence of gene exchange with the phylogenetically distant cyanobacteria, particularly for sensory and signal transduction proteins. because these are ... | 2016 | 27895624 |
| importance of fumarate and nitrate reduction regulatory protein for intestinal proliferation of vibrio vulnificus. | the sepsis caused by vibrio vulnificus is characterized by an average incubation period of 26 h and a high mortality rate exceeding 50%. the fast growth and dissemination of v. vulnificus in vivo lead to poor clinical outcomes in patients. therefore, elucidation of the proliferation mechanisms of this organism in vivo may lead to the development of an effective therapeutic strategy. in this study, we focused on the low oxygen concentration in the intestinal milieu because of its drastic differen ... | 2017 | 27915250 |
| cross-protection against vibrio cholerae infection by monoclonal antibodies against vibrio vulnificus rtxa1/martxvv. | gram-negative vibrio species secrete multifunctional autoprocessing repeats-in-toxin (martx) toxins associated with bacterial pathogenesis. here, the cross-reactivity and cross-protectivity of mabs against v. vulnificus rtxa1/martxvv was evaluated. passive administration of any of these mabs (21ra, 24ra, 46ra, 47ra and 50ra) provided strong protection against lethal v. cholerae infection. interestingly, 24ra and 46ra, which map to the cysteine protease domain of v. cholerae martxvc , inhibited c ... | 2016 | 27921342 |
| effects of pyrogallol on growth and cytotoxicity of wild-type and katg mutant strains of vibrio vulnificus. | vibrio vulnificus is a causative agent of fatal septicemia and necrotic wound infection and the pathogen infection became an important public health problem in many counties. vibrio vulnificus causes rtxa1 toxin-induced acute cell death. we tried to identify natural products that inhibit the acute cytotoxicity of v. vulnificus using a lactate hydrogenase assay. a polyphenol pyrogallol protected hela cells from v. vulnificus-induced cytotoxicity. pyrogallol also decreased the growth of v. vulnifi ... | 2016 | 27936080 |
| the membrane localization domains of two distinct bacterial toxins form a 4-helix-bundle in solution. | membrane localization domain (mld) was first proposed for a 4-helix-bundle motif in the crystal structure of the c1 domain of pasteurella multocida toxin (pmt). this structure motif is also found in the crystal structures of several clostridial glycosylating toxins (tcda, tcdb, tcsl, and tcna). the ras/rap1-specific endopeptidase (rrsp) module of the multifunctional autoprocessing repeats-in-toxins (martx) toxin produced by vibrio vulnificus has sequence homology to the c1-c2 domains of pmt, inc ... | 2017 | 27977897 |
| cmc stabilized nano silver synthesis, characterization and its antibacterial and synergistic effect with broad spectrum antibiotics. | in the present study silver nanoparticles were synthesized by reduction of agno3 using aqueous cmc solution, which acts as both reducing and capping agent. the formation of agno3 nanoparticles was observed visually by color change and these nanoparticles were characterized through uv-vis spectroscopy, ftir, xrd, sem, eds and afm. the ftir peaks observed to be ranging from 3300 to 605cm(-1). the afm image clearly showed the surface morphology of well dispersed nanoparticles. sem image illustrates ... | 2017 | 28024537 |
| development of a regulatable expression system for the functional study of vibrio vulnificus essential genes. | we developed a regulatable gene expression system for vibrio vulnificus, which contains a laciq-ptrc cassette. monomeric red fluorescence protein (mrfp) was used as a reporter to test this system. the results showed that this system tightly controlled the expression of mrfp without leaky expression and was suitable for the controlled expression of genes encoding recombinant proteins in v. vulnificus. to demonstrate the utility of this system, a dominant negative form of v. vulnificus vvmo6_rs049 ... | 2017 | 28044234 |
| successful salvage and reconstruction of a finger threatened by vibrio vulnificus necrotising fasciitis using fenestrated-type artificial dermis and three steps of topical negative pressure wound therapy. | vibrio vulnificus can cause severe skin and soft tissue infection (ssti). the pathogen is an opportunistic marine bacterium that is likely to infect patients with chronic liver disease, patients in an immunocompromised state, and those in end-stage renal disease. v. vulnificus gains entry through soft tissues by direct penetration of a wound by infected marine organisms, such as raw oysters, shellfish and other seafood, or by exposing a wound to contaminated water. despite its ease of entry, v. ... | 2017 | 28052529 |
| vibrio vulnificus: from oyster colonist to human pathogen. | 2017 | 28056111 | |
| first multi-year retrospective study on vibrio parhaemolyticus and vibrio vulnificus prevalence in ruditapes philippinarum harvested in sacca di goro, italy. | the present work describes a retrospective study aiming to verify a possible correlation between the environmental conditions (temperature, salinity and dissolved oxygen), the abundance of vibrio spp., and the prevalence of v. parahaemolyticus and v. vulnificus in the manila clam r. philippinarum harvested in sacca di goro, emilia-romagna region, northern italy. on the whole, 104 samples, collected in the period 2007-2015 and submitted to microbiological analyses (isolation and genotyping), have ... | 2016 | 28058248 |
| the effector domain region of the vibrio vulnificus martx toxin confers biphasic epithelial barrier disruption and is essential for systemic spread from the intestine. | vibrio vulnificus causes highly lethal bacterial infections in which the multifunctional autoprocessing repeats-in-toxins (martx) toxin product of the rtxa1 gene is a key virulence factor. martx toxins are secreted proteins up to 5208 amino acids in size. conserved martx n- and c-terminal repeat regions work in concert to form pores in eukaryotic cell membranes, through which the toxin's central region of modular effector domains is translocated. upon inositol hexakisphosphate-induced activation ... | 2017 | 28060924 |
| study of the antimicrobial activity of tilapia piscidin 3 (tp3) and tp4 and their effects on immune functions in hybrid tilapia (oreochromis spp.). | to address the growing concern over antibiotic-resistant microbial infections in aquatic animals, we tested several promising alternative agents that have emerged as new drug candidates. specifically, the tilapia piscidins are a group of peptides that possess antimicrobial, wound-healing, and antitumor functions. in this study, we focused on tilapia piscidin 3 (tp3) and tp4, which are peptides derived from oreochromis niloticus, and investigated their inhibition of acute bacterial infections by ... | 2017 | 28085905 |
| mechanism of anti-vibrio activity of marine probiotic strain bacillus pumilus h2, and characterization of the active substance. | vibriosis is a major epizootic disease that impacts free-living and farmed fish species worldwide. use of probiotics is a promising approach for prevention of vibrio infections in aquaculture. a probiotic anti-vibrio strain, bacillus pumilus h2, was characterized, and the mechanism of its effect was investigated. all 29 vibrio strains tested were growth-inhibited by h2. the anti-vibrio substance present in cell-free supernatant of h2 was purified and characterized by reversed-phase hplc. minimum ... | 2017 | 28097594 |
| high salinity relaying to reduce vibrio parahaemolyticus and vibrio vulnificus in chesapeake bay oysters (crassostrea virginica). | cases of vibrio infections in the united states have tripled from 1996 to 2009 and these infections are most often associated with the consumption of seafood, particularly oysters (crassostrea virginica). information is needed on how to reduce numbers of vibrio parahaemolyticus and vibrio vulnificus in bi-valve molluscan shellfish (for example, oysters). the purpose of this study was to evaluate the effectiveness of high salinity relaying or treatment in recirculating aquaculture systems (rass) ... | 2017 | 28099766 |
| transcriptome analysis of the effect of polyunsaturated fatty acids against vibrio vulnificus infection in oreochromis niloticus. | vibrio vulnificus infection causes severe economic losses in oreochromis niloticus aquaculture by inducing pro-inflammatory cytokines, that lead to inflammation and mortality. omega-3 fatty acids, such as docosahexaenoic acid (dha) and eicosapentaenoic acid (epa), have been reported for their anti-inflammatory and antibacterial abilities in murine and zebrafish models. however, the anti-inflammatory and antibacterial functions of dha and epa in commercial aquaculture organisms such as oreochromi ... | 2017 | 28108339 |
| regulation systems of protease and hemolysin production in vibrio vulnificus. | vibrio vulnificus, a gram-negative halophilic estuarine bacterium, is an opportunistic human pathogen that causes rapidly progressive fatal septicemia and necrotizing wound infection. this species also causes hemorrhagic septicemia called vibriosis in cultured eels. it has been proposed that a range of virulence factors play roles in pathogenesis during human and/or eel infection. among these factors, a metalloprotease (v. vulnificus protease [vvp]) and a cytolytic toxin (v. vulnificus hemolysin ... | 2017 | 28111826 |
| role of non-coding regulatory rna in the virulence of human pathogenic vibrios. | in recent decades, the identification of small non-coding rnas in bacteria has revealed an important regulatory mechanism of gene expression involved in the response to environmental signals and to the control of virulence. in the family vibrionaceae, which includes several human and animal pathogens, small non-coding rnas (srnas) are closely related to important processes including metabolism, quorum sensing, virulence, and fitness. studies conducted in silico and experiments using microarrays ... | 2016 | 28123382 |
| iutb participates in the ferric-vulnibactin utilization system in vibrio vulnificus m2799. | vibrio vulnificus, an opportunistic pathogen that causes a serious, often fatal, infection in humans, requires iron for its growth. this bacterium utilizes iron from the environment via the vulnibactin-mediated iron uptake system. the mechanisms of vulnibactin biosynthesis, vulnibactin export, and ferric-vulnibactin uptake systems have been reported, whereas the ferric-vulnibactin reduction mechanism in the cell remains unclear. the results of our previous study showed that vuub, a member of the ... | 2017 | 28150143 |
| two-step enhanced cancer immunotherapy with engineered salmonella typhimurium secreting heterologous flagellin. | we report a method of cancer immunotherapy using an attenuated salmonella typhimurium strain engineered to secrete vibrio vulnificus flagellin b (flab) in tumor tissues. engineered flab-secreting bacteria effectively suppressed tumor growth and metastasis in mouse models and prolonged survival. by using toll-like receptor 5 (tlr5)-negative colon cancer cell lines, we provided evidence that the flab-mediated tumor suppression upon bacterial colonization is associated with tlr5-mediated host react ... | 2017 | 28179508 |
| ligand-modulated folding of the full-length adenine riboswitch probed by nmr and single-molecule fret spectroscopy. | the full-length translation-regulating add adenine riboswitch (asw) from vibrio vulnificus has a more complex conformational space than its isolated aptamer domain. in addition to the predicted apo (apoa) and holo conformation that feature the conserved three-way junctional purine riboswitch aptamer, it adopts a second apo (apob) conformation with a fundamentally different secondary structure. here, we characterized the ligand-dependent conformational dynamics of the full-length add asw by nmr a ... | 2017 | 28204648 |
| high pressure processing of bivalve shellfish and hpp's use as a virus intervention. | bivalve shellfish readily bioconcentrate pathogenic microbes and substance, such as algal and dinoflagulate toxins, fecal viruses and bacteria, and naturally present vibrio bacteria. high pressure processing (hpp) is currently used as an intervention for vibrio vulnificus bacteria within molluscan shellfish and its potential to inactivate food-borne viruses and bacteria are discussed. mechanisms of action of high pressure against bacteria and viruses, as well as how time of pressure application, ... | 2014 | 28234323 |
| plesiomonas shigelloides periprosthetic knee infection after consumption of raw oysters. | periprosthetic infections are a leading cause of morbidity after total joint arthroplasty. common pathogens include staphylococcus aureus, streptococcus, enterococcus, escherichia coli, and pseudomonas aeruginosa. however, there are many cases in which rare bacteria are isolated. this case report describes a periprosthetic knee infection caused by plesiomonas shigelloides. in the united states, p shigelloides and 2 other vibrionaceae family members, vibrio vulnificus and vibrio parahaemolyticus, ... | 2017 | 28235124 |
| tc38, a teleost tfpi-2 peptide that kills bacteria via penetration of the cell membrane and interaction with nucleic acids. | tissue factor pathway inhibitor 2 (tfpi-2) is an analog of tfpi-1 and a potent endogenous inhibitor of tissue factor (tf)-mediated blood coagulation. recent reports have proven that the c-terminal of tfpi-2 peptides in humans and several other vertebrates possesses antibacterial activity against gram-positive and gram-negative bacteria. in our previous study, we reported that the tfpi-2 peptide, tc38 in tongue sole (cynoglossus semilaevis) was active against micrococcus luteus. in this study, we ... | 2017 | 28263880 |
| the hydrogen peroxide hypersensitivity of oxyr2 in vibrio vulnificus depends on conformational constraints. | most gram-negative bacteria respond to excessive levels of h2o2 using the peroxide-sensing transcriptional regulator oxyr, which can induce the expression of antioxidant genes to restore normality. vibrio vulnificus has two distinct oxyrs (oxyr1 and oxyr2), which are sensitive to different levels of h2o2 and induce expression of two different peroxidases, prx1 and prx2. while oxyr1 has both high sequence similarity and comparable h2o2 sensitivity to other oxyr proteins, oxyr2 exhibits limited se ... | 2017 | 28264933 |
| incidences of waterborne and foodborne diseases after meteorologic disasters in south korea. | climate change could increase the number of regions affected by meteorologic disasters. meteorologic disasters can increase the risk of infectious disease outbreaks, including waterborne and foodborne diseases. although many outbreaks of waterborne diseases after single disasters have been analyzed, there have not been sufficient studies reporting comprehensive analyses of cases occurring during long-term surveillance after multiple disasters, which could provide evidence of whether meteorologic ... | 2017 | 28283139 |
| occurrence of clinical genotype vibrio vulnificus in clam samples in mangalore, southwest coast of india. | vibrio vulnificus is an opportunistic human pathogen causing gastroenteritis, wound infection and primary septicemia. v. vulnificus population has been divided into subpopulations based on their phenotype and genotype characteristics. in this study, 38.5% (10/26) of clam (meretrix meretrix) samples obtained from mangalore markets were seen to harbor v. vulnificus. biochemical characterization of v. vulnificus isolates showed the strains to belong to biotype 1 phenotype. genotyping of strains usi ... | 2017 | 28298693 |
| expression, purification and enzymatic characterization of undecaprenyl pyrophosphate phosphatase from vibrio vulnificus. | undecaprenyl pyrophosphate phosphatase (uppp), a cell membrane integral enzyme, catalyzes the dephosphorylation of undecaprenyl pyrophosphate to undecaprenyl phosphate, which is an essential carrier lipid in bacterial cell wall synthesis. we previously purified e. coli uppp and concluded that its catalytic site is likely located in the periplasm. to search for additional natural uppp homologs to elucidate what constitutes a common catalytic mechanism and to gain a better chance of obtaining high ... | 2017 | 28302513 |
| occurrence and virulence properties of vibrio and salinivibrio isolates from tropical lagoons of the southern caribbean sea. | the vibrionaceae are gram-negative bacteria present in marine and estuarine environments worldwide, including several species known as important pathogens to humans and aquatic organisms. the aim of this research was to investigate the occurrence and virulence properties of vibrio and salinivibrio isolated from lagoons at cuare wildlife refuge and margarita island in the southern caribbean sea. water, plankton and oyster samples were collected during october 2011 and march 2012 and examined by s ... | 2017 | 28324231 |
| characterization of antibiotic resistant and enzyme producing bacterial strains isolated from the arabian sea. | marine bacteria are known to produce many bioactive molecules and extracellular enzymes of commercial importance. we have investigated the bacterial diversity of the coastal area of karwar, karnataka state, india. among these bacterial isolates, five bacterial strains were selected and identified by their morphological, biochemical characteristics and phylogenetic analysis based on 16s rrna gene sequences. the identified bacterial isolates, bacillus toyonensis pntb1, lysinibacillus sphaericus pt ... | 2016 | 28330094 |
| clinical features and treatment of patients with vibrio vulnificus infection. | infections with vibrio vulnificus are commonly fatal, and the speed and accuracy of diagnosis and treatment is directly linked to mortality. the main aims of this study were to investigate the clinical characteristics of six patients with v. vulnificus infections retrospectively and to determine the effect of treatment with tigecycline (tgc) alone compared with doxycycline plus ceftazidime (dox/caz). | 2017 | 28347850 |
| antimicrobial activities of secondary metabolites and phylogenetic study of sponge endosymbiotic bacteria, bacillus sp. at agatti island, lakshadweep archipelago. | twenty-one species of sponges were recorded under the class of demospongiae and calcareous sponges of which 19 species were new to agatti reef. a total of 113 sponge endosymbiotic bacterial strains were isolated from twenty-one species of sponges and screened for antimicrobial activity. five bacterial strains of sponge endosymbiotic bacteria (seb) namely seb32, seb33, seb36, seb43 and seb51 showed antimicrobial activity against virulent marine fish pathogens such as vibrio alginolyticus, vibrio ... | 2016 | 28352539 |
| mechanisms for pseudoalteromonas piscicida-induced killing of vibrios and other bacterial pathogens. | pseudoalteromonas piscicida are gram-negative gammaproteobacteria found in the marine environment. three strains of pigmented p. piscicida were isolated from seawater and partially characterized by inhibition studies, electron microscopy, and proteolytic enzyme production. growth inhibition and death occurred around colonies of p. piscicida on lawns of the naturally-occurring marine pathogens vibrio vulnificus, v. parahaemolyticus, v. cholerae, photobacterium damselae and shewanella algae inhibi ... | 2017 | 28363962 |
| responses of mytilus galloprovincialis hemocytes to environmental strains of vibrio parahaemolyticus, vibrio alginolyticus, vibrio vulnificus. | marine bivalves are exposed to different types of bacteria in the surrounding waters, in particular of the vibrio genus. in the hemocytes of the mussel mytilus spp. immune responses to different vibrios have been largely characterized. however, little information is available on the hemocyte responses to human pathogenic vibrios commonly detected in coastal waters and bivalve tissues that are involved in seafood-borne diseases. | 2017 | 28390964 |
| crystal structure of the regulatory domain of aphb from vibrio vulnificus, a virulence gene regulator. | the transcriptional activator aphb has been implicated in acid resistance and pathogenesis in the food borne pathogens vibrio vulnificus and vibrio cholerae. to date, the full-length aphb crystal structure of v. cholerae has been determined and characterized by a tetrameric assembly of aphb consisting of a dna binding domain and a regulatory domain (rd). although acidic ph and low oxygen tension might be involved in the activation of aphb, it remains unknown which ligand or stimulus activates ap ... | 2017 | 28427249 |
| corrigendum: assessing the function of stas domain protein sypa in vibrio fischeri using a comparative analysis. | [this corrects the article on p. 760 in vol. 6, pmid: 26284045.]. | 2017 | 28450861 |
| substrate recognition of martx ras/rap1-specific endopeptidase. | ras/rap1-specific endopeptidase (rrsp) is a cytotoxic effector domain of the multifunctional autoprocessing repeats-in-toxin (martx) toxin of highly virulent strains of vibrio vulnificus. rrsp blocks ras-mapk kinase signaling by cleaving ras and rap1 within the switch i region between y32 and d33. although the rrsp processing site is highly conserved among small gtpases, only ras and rap1 have been identified as proteolytic substrates. here we report that residues y32 and d33 at the scissile bon ... | 2017 | 28459538 |
| effects of ambient exposure, refrigeration, and icing on vibrio vulnificus and vibrio parahaemolyticus abundances in oysters. | vibrio vulnificus (vv) and v. parahaemolyticus (vp) illnesses are typically acquired through the consumption of raw molluscan shellfish, particularly oysters. as vibrio spp. are naturally-occurring bacteria, one means of mitigation of illness is achieved by limiting post-harvest growth. in this study, effects of ambient air storage, refrigeration, and icing of oysters on vibrio spp. abundances were examined at two sites in alabama (al) [dog river (dr) and cedar point (cp)] and one site in delawa ... | 2017 | 28482282 |
| the blue fluorescent protein from vibrio vulnificus ckm-1 is a useful reporter for plant research. | the mbfp is an improved variant of nadph-dependent blue fluorescent protein that was originally identified from the non-bioluminescent pathogenic bacteria vibrio vulnificus ckm-1. to explore the application of mbfp in plants, the mbfp gene expression was driven by one of the three promoters, namely, leaf-specific (rbcs), hypoxia-inducible (adh) or auxin-inducible (dr5) promoters, in different plant tissues such as leaves, roots and flowers under diverse treatments. in addition, the expressed mbf ... | 2014 | 28510958 |
| different abundance and correlational patterns exist between total and presumed pathogenic vibrio vulnificus and v. parahaemolyticus in shellfish and waters along the north carolina coast. | monitoring of vibrio vulnificus and v. parahaemolyticus abundance is pertinent due to the ability of these species to cause disease in humans through aquatic vectors. previously, we performed a multiyear investigation tracking vibrio spp. levels in five sites along the southeastern north carolina coast. from february 2013 to october 2015, total v. vulnificus and v. parahaemolyticus abundance was measured in water, oysters and clams. in the current study, pathogenic subpopulations were identified ... | 2017 | 28531284 |
| site-specific his/asp phosphoproteomic analysis of prokaryotes reveals putative targets for drug resistance. | phosphorylation of amino acid residues on proteins is an important and common post-translational modification in both eukaryotes and prokaryotes. most research work has been focused on phosphorylation of serine, threonine or tyrosine residues, whereas phosphorylation of other amino acids are significantly less clear due to the controversy on their stability under standard bioanalytical conditions. | 2017 | 28545444 |
| both polarity and aromatic ring in the side chain of tryptophan 246 are involved in binding activity of vibrio vulnificus hemolysin to target cells. | vibrio vulnificus secretes a hemolysin/cytolysin (vvh) that induces cytolysis against a variety of mammalian cells by forming pores on the cellular membrane. vvh is known to bind to the cellular membrane as a monomer, and then convert to a pore-forming oligomer. however, the structural basis for binding of this toxin to target cells remains unknown. we show here that the polarity and indole ring on the side chain of trp 246 (w246) of vvh, which sits on a bottom loop, participates in binding to c ... | 2017 | 28546115 |
| vibrio vulnificus septic shock due to a contaminated tattoo. | we present a case of vibrio vulnificus septic shock and cellulitis in a patient with chronic liver disease that occurred after obtaining a leg tattoo with subsequent seawater exposure in the gulf of mexico. initial suspicion for v. vulnificus was high and he was started on empiric doxycycline and ceftriaxone at admission. blood and wound cultures grew oxidase positive and comma-shaped gram-negative rods ultimately confirmed to be v. vulnificus. despite aggressive initial treatment, the patient d ... | 2017 | 28551603 |
| vibrio vulnificus: an environmental and clinical burden. | vibrio vulnificus is a gram negative, rod shaped bacterium that belongs to the family vibrionaceae. it is a deadly, opportunistic human pathogen which is responsible for the majority of seafood-associated deaths worldwide. v. vulnificus infection can be fatal as it may cause severe wound infections potentially requiring amputation or lead to sepsis in susceptible individuals. treatment is increasingly challenging as v. vulnificus has begun to develop resistance against certain antibiotics due to ... | 2017 | 28620366 |
| comparative visualization of the rna suboptimal conformational ensemble in vivo. | when a ribonucleic acid (rna) molecule folds, it often does not adopt a single, well-defined conformation. the folding energy landscape of an rna is highly dependent on its nucleotide sequence and molecular environment. cellular molecules sometimes alter the energy landscape, thereby changing the ensemble of likely low-energy conformations. the effects of these energy landscape changes on the conformational ensemble are particularly challenging to visualize for large rnas. we have created a robu ... | 2017 | 28625696 |
| surface hypothermia predicts murine mortality in the intragastric vibrio vulnificus infection model. | the gram-negative bacterium vibrio vulnificus can cause severe disease in humans who consume undercooked, contaminated seafood. to study food-borne v. vulnificus disease in the laboratory, mouse virulence studies predominantly use death as the primary experimental endpoint because behaviorally based moribund status does not consistently predict lethality. this study assessed ventral surface temperature (vst) and its association with mouse survival during v. vulnificus virulence studies as an eff ... | 2017 | 28629317 |
| a double-quadratic model for predicting vibrio species in water environments of japan. | vibrio spp. are natural inhabitants of marine and estuarine environments. vibrio cholerae, vibrio parahaemolyticus, and vibrio vulnificus are the major infectious agents for humans. their densities are affected by environmental factors such as water temperature and salinity. the detailed contribution of each factor still remains to be elucidated. here we conducted multi-coastal study in a 21-month period to examine relationships between environmental factors and v. cholerae, v. parahaemolyticus ... | 2017 | 28646477 |
| the proline variant of the w[f/l/m][t/s]r c-di-gmp binding motif suppresses dependence on signal-association for regulator function. | vibrio vulnificus is an estuarine bacteria and potent opportunistic human pathogen. it enters the food-chain by asymptomatically colonizing a variety of marine organisms, most notably oysters. expression of the brp-encoded extracellular polysaccharide, which enhances cell-surface adherence, is regulated by c-di-gmp and the activator brpt. the v. cholerae and v. parahaemolyticus homologs, vpst and cpsq, directly bind c-di-gmp via a novel w[f/l/m][t/s]r motif and c-di-gmp binding is absolutely req ... | 2017 | 28652300 |
| vibrio vulnificus tonsillitis after swimming in the gulf of mexico. | 2017 | 28659373 | |
| recreational waterborne illnesses: recognition, treatment, and prevention. | illness after recreational water activities can be caused by a variety of agents, including bacteria, viruses, parasites, algae, and even chlorine gas. these illnesses are more common in summer. waterborne illnesses are underreported because most recreational activity occurs in unsupervised venues or on private property, and participants tend to disperse before illness occurs. symptoms of waterborne illness are primarily gastrointestinal, but upper respiratory and skin manifestations also occur. ... | 2017 | 28671392 |
| from sea to bloodstream: vibrio vulnificus sepsis. | 2017 | 28693913 | |
| effects of desiccation practices of cultured atlantic oysters (crassostrea virginica) on vibrio spp. in portersville bay, alabama, usa. | the expansion of off-bottom aquaculture to the gulf of mexico has raised public health concerns for human health officials. high temperatures in the gulf of mexico are associated with high levels of vibrio parahaemolyticus and vibrio vulnificus. routine desiccation practices associated with off-bottom aquaculture expose oysters to ambient air, allowing vibrio spp. to proliferate in the closed oyster. currently, there is limited research on the length of time needed for vibrio spp. levels in desi ... | 2017 | 28696147 |
| skin manifestations of primary vibrio vulnificus septicemia. | 2017 | 28719320 | |
| vibrio vulnificus induces mtor activation and inflammatory responses in macrophages. | vibrio vulnificus (v. vulnificus), a gram-negative marine bacterium, can cause life-threatening primary septicemia, especially in patients with liver diseases. how v. vulnificus affects the liver and how it acts on macrophages are not well understood. in this report, we demonstrated that v. vulnificus infection causes a strong inflammatory response, marked expansion of liver-resident macrophages, and liver damage in mice. we demonstrated further that v. vulnificus activates mtor in macrophages a ... | 2017 | 28719654 |
| martx toxin in the zoonotic serovar of vibrio vulnificus triggers an early cytokine storm in mice. | vibrio vulnificus biotype 2-serovar e is a zoonotic clonal complex that can cause death by sepsis in humans and fish. unlike other biotypes, bt2 produces a unique type of martxvv (multifunctional-autoprocessive-repeats-in-toxin; rtxa13), which is encoded by a gene duplicated in the pvvbt2 plasmid and chromosome ii. in this work, we analyzed the activity of this toxin and its role in human sepsis by performing in vitro, ex vivo, and in vivo assays. first, we demonstrated that the acd domain, pres ... | 2017 | 28775962 |
| distribution and antimicrobial susceptibility of vibrio species associated with zooplankton in coastal area of korea. | vibrio parahaemolyticus and v. vulnificus are the most common pathogens causing seafood-borne illnesses in korea. this study determines the abundance and antimicrobial resistance of pathogenic vibrio species in seawater and zooplankton samples from the geoje island coast in korea, which is an important area for coastal fisheries, the fishing industry, and tourism. the two vibrio species were detected more in mesozooplankton samples than in seawater samples. v. parahaemolyticus isolates showed gr ... | 2017 | 28781188 |
| lrp, a global regulator, regulates the virulence of vibrio vulnificus. | an attenuated mutant (designated ny303) of vibrio vulnificus, which causes serious wound infection and septicemia in humans, was isolated fortuitously from a clinical strain yj016. this mutant was defective in cytotoxicity, migration on soft agar and virulence in the mouse. the purpose of this study was to map the mutation in this attenuated mutant and further explore how the gene thus identified is involved in virulence. | 2017 | 28800764 |
| variable virulence of biotype 3 vibrio vulnificus due to martx toxin effector domain composition. | vibrio vulnificus is an environmental organism that causes septic human infections characterized by high morbidity and mortality. the annual incidence and global distribution of this pathogen are increasing as ocean waters warm. clinical strains exhibit variations in the primary virulence toxin, suggesting a potential for the emergence of new strains with altered virulence properties. a clonal outbreak of tilapia-associated wound infections in israel serves as a natural experiment for the sudden ... | 2017 | 28815212 |
| vibrio vulnificus vvha induces th1 and tfh cells to proliferate against vibrio vulnificus in a mouse model of infection. | to characterize the roles of vvha in host's acquired immune response to vibrio vulnificus infection. | 2017 | 28816516 |
| anti-biofilm effects of anthranilate on a broad range of bacteria. | anthranilate, one of tryptophan degradation products has been reported to interfere with biofilm formation by pseudomonas aeruginosa. here, we investigated the effects of anthranilate on biofilm formation by various bacteria and the mechanisms responsible. anthranilate commonly inhibited biofilm formation by p. aeruginosa, vibrio vulnificus, bacillus subtilis, salmonella enterica serovar typhimurium, and staphylococcus aureus, and disrupted biofilms preformed by these bacteria. because anthranil ... | 2017 | 28819217 |
| vibrio vulnificus martx cytotoxin causes inactivation of phagocytosis-related signaling molecules in macrophages. | vibrio vulnificus is a marine bacterial species that causes opportunistic infections manifested by serious skin lesions and fulminant septicemia in humans. we have previously shown that the multifunctional autoprocessing repeats in toxin (martxvv1) of a biotype 1 v. vulnificus strain promotes survival of this organism in the host by preventing it from engulfment by the phagocytes. the purpose of this study was to further explore how martxvv1 inhibits phagocytosis of this microorganism by the mac ... | 2017 | 28822352 |
| occurrence of vibrio species, beta-lactam resistant vibrio species, and indicator bacteria in ballast and port waters of a tropical harbor. | ballast water discharges are potential sources for the spread of invasive and pathogenic aquatic organisms. ballast waters from six ships docked in the port of singapore were tested to determine if indictor organisms fell within proposed standards for ballast water discharge according to regulation d-2 of the ballast water management convention (bwmc) guidelines. vibrio species were cultured on media supplemented with beta-lactam antibiotics to determine the presence of antibiotic resistant vibr ... | 2017 | 28822933 |
| clinical usefulness of real-time polymerase chain reaction for the diagnosis of vibrio vulnificus infection using skin and soft tissues. | vibrio vulnificus is a halophilic gram-negative bacillus isolated in seawater, fish, and shellfish. infection by v. vulnificus is the most severe food-borne infection reported in the united states of america. here, we aimed to examine the clinical usefulness of polymerase chain reaction (pcr) using tissue specimens other than blood samples as a diagnostic tool for v. vulnificus infection. a retrospective study was conducted with patients who underwent real-time pcr of toxr in both blood and skin ... | 2017 | 28829729 |
| previously undescribed antibacterial polyketides from heterotrophic bacillus amyloliquefaciens associated with seaweed padina gymnospora. | a heterotrophic marine bacterium bacillus amyloliquefaciens isolated from seaweed padina gymnospora exhibited broad spectra of antibacterial activities against pathogenic bacteria aeromonas hydrophila, vibrio harveyi, vibrio vulnificus, and vibrio parahaemolyticus. the seaweed-associated b. amyloliquefaciens was recognized to possess functional type i polyketide synthase-1 (pks-1) gene, and was used to isolate four homologous compounds with polyketide frameworks. the compounds were characterized ... | 2017 | 28842846 |
| a vibrio vulnificus vvpm induces il-1β production coupled with necrotic macrophage death via distinct spatial targeting by anxa2. | an inflammatory form of phagocyte death evoked by the gram-negative bacterium vibrio (v.) vulnificus (wt) is one of hallmarks to promote their colonization, but the virulence factor and infectious mechanism involved in this process remain largely unknown. here, we identified extracellular metalloprotease vvpm as a new virulence factor and investigated the molecular mechanism of vvpm which acts during the regulation of the inflammatory form of macrophage death and bacterial colonization. mutation ... | 2017 | 28848713 |