Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| analysis of the role of bphs/hrh1 in the genetic control of responsiveness to pertussis toxin. | in vivo intoxication with bordetella pertussis toxin (ptx) elicits a variety of physiological responses including a marked leukocytosis, disruption of glucose regulation, adjuvant activity, alterations in vascular function, hypersensitivity to vasoactive agents, and death. we recently identified bphs, the locus controlling ptx-induced hypersensitivity to the vasoactive amine histamine, as the histamine h(1) receptor (hrh1). in this study bphs congenic mice and mice with a disrupted hrh1 gene wer ... | 2003 | 12595443 |
| reduced glutathione is required for pertussis toxin secretion by bordetella pertussis. | the abilities of cysteine-containing compounds to support growth of bordetella pertussis and influence pertussis toxin transcription, assembly, and secretion were examined. cysteine is an essential amino acid for b. pertussis and must be present for protein synthesis and bacterial growth. however, cysteine can be metabolized to sulfate, and high concentrations of sulfate can selectively inhibit transcription of the virulence factors, including pertussis toxin, via the bvgas two-component regulat ... | 2003 | 12595447 |
| attenuated virulence of a burkholderia cepacia type iii secretion mutant in a murine model of infection. | type iii secretion systems are utilized by a number of gram-negative bacterial pathogens to deliver virulence-associated proteins into host cells. using a pcr-based approach, we identified homologs of type iii secretion genes in the gram-negative bacterium burkholderia cepacia, an important pulmonary pathogen in immunocompromised patients and patients with cystic fibrosis. one of the genes, designated bscn, encodes a member of a family of atp-binding proteins believed to generate energy driving ... | 2003 | 12595458 |
| a conserved region within the bordetella pertussis autotransporter brka is necessary for folding of its passenger domain. | autotransporter secretion represents a unique mechanism that gram-negative bacteria employ to deliver proteins to their cell surface. brka is a bordetella pertussis autotransporter protein that mediates serum resistance and contributes to adherence of the bacterium to host cells. brka is a 103 kda protein that is cleaved to form a 73 kda alpha-domain and a 30 kda beta domain. the alpha domain, also referred to as the passenger domain, is responsible for the effector functions of the protein, whe ... | 2003 | 12603741 |
| systemic administration of bordetella pertussis enhances pulmonary sensitization to house dust mite in juvenile rats. | the incidence of allergies and asthma has increased significantly in the past few decades. the objectives of this study were to establish an allergy model in weanling rats to more closely reflect the developing immune system of children, and to determine whether systemic administration of inactivated bordetella pertussis could enhance pulmonary and systemic immune responses to locally administered house dust mite antigen (hdm). three-week old female brown norway rats were sensitized with 10 micr ... | 2003 | 12604840 |
| type iv transporters of pathogenic bacteria. | type iv transporters are produced by several bacterial pathogens such as agrobacterium tumefaciens, bordetella pertussis, brucella spp., bartonella henselae, helicobacter pylori and legionella pneumophila. these transporters are critical for the pathogenic process in that they export important virulence factors across the membranes of the bacteria. although the virulence factors that are exported by these transporters can be either nucleic acid or protein, the general mechanism of transport appe ... | 2003 | 12615216 |
| in vitro activity of fluoroquinolones against erythromycin-susceptible and -resistant bordetella pertussis. | 2003 | 12615884 | |
| transcriptome analysis of sinorhizobium meliloti during symbiosis. | rhizobia induce the formation on specific legumes of new organs, the root nodules, as a result of an elaborated developmental program involving the two partners. in order to contribute to a more global view of the genetics underlying this plant-microbe symbiosis, we have mined the recently determined sinorhizobium meliloti genome sequence for genes potentially relevant to symbiosis. we describe here the construction and use of dedicated nylon macroarrays to study simultaneously the expression of ... | 2003 | 12620125 |
| collaborative study for the establishment of a european phamacopoeia biological reference preparation for bordetella pertussis mouse antiserum for serological potency testing of acellular pertussis vaccines. | a collaborative study was organised by the european directorate for the quality of medicines (edqm) to assess the suitability of a candidate mouse antiserum as a european pharmacopoeia biological reference preparation (brp) for acellular pertussis vaccine potency testing. the candidate antiserum was obtained by immunising mice with a five-component acellular pertussis vaccine: pertussis toxin (pt), filamentous haemagglutinin (fha), pertactin (prn) and fimbrial 2/fimbrial 3 (fim 2&3). the study h ... | 2003 | 12623057 |
| identification of a mutation associated with erythromycin resistance in bordetella pertussis: implications for surveillance of antimicrobial resistance. | erythromycin treatment failures and in vitro resistance of bordetella pertussis have been reported on several occasions in the past few years, but the mechanism of resistance has not been described. one potential mechanism, genetic modification of the erythromycin-binding site on the 23s rrna of the 50s ribosomal subunit, has been observed in other bacteria. to explore this possibility, we amplified the portion of the 23s rrna gene encoding the central loop of domain v. dna sequencing and restri ... | 2003 | 12624047 |
| genotypes at the internal transcribed spacers of the nuclear rrna operon of pneumocystis jiroveci in nonimmunosuppressed infants without severe pneumonia. | the frequency of pneumocystis jiroveci (human-derived pneumocystis) in immunocompetent infants developing acute respiratory syndromes has recently been evaluated and has been shown to be close to 25%. until now, there have been no data on the genomic characteristics of the fungus in these patients, while molecular typing of p. jiroveci organisms was mostly performed with samples from immunosuppressed patients with pneumocystosis (pneumocystis carinii pneumonia [pcp]). the present report describe ... | 2003 | 12624048 |
| role of adhesin release for mucosal colonization by a bacterial pathogen. | pathogen attachment is a crucial early step in mucosal infections. this step is mediated by important virulence factors called adhesins. to exert these functions, adhesins are typically surface-exposed, although, surprisingly, some are also released into the extracellular milieu, the relevance of which has previously not been studied. to address the role of adhesin release in pathogenesis, we used bordetella pertussis as a model, since its major adhesin, filamentous hemagglutinin (fha), partitio ... | 2003 | 12629063 |
| in silico pattern-based analysis of the human cytomegalovirus genome. | more than 200 open reading frames (orfs) from the human cytomegalovirus genome have been reported as potentially coding for proteins. we have used two pattern-based in silico approaches to analyze this set of putative viral genes. with the help of an objective annotation method that is based on the bio-dictionary, a comprehensive collection of amino acid patterns that describes the currently known natural sequence space of proteins, we have reannotated all of the previously reported putative gen ... | 2003 | 12634390 |
| human metapneumovirus in severe respiratory syncytial virus bronchiolitis. | reverse transcription-polymerase chain reaction was used to detect segments of the m (matrix), n (nucleoprotein), and f (fusion) genes of human metapneumovirus in bronchoalveolar fluid from 30 infants with severe respiratory syncytial virus bronchiolitis. seventy percent of them were coinfected with metapneumovirus. such coinfection might be a factor influencing the severity of bronchiolitis. | 2003 | 12643835 |
| zccr--a merr-like regulator from bordetella pertussis which responds to zinc, cadmium, and cobalt. | a transcriptional regulator of the merr family encoded by bordetella pertussis was characterized in escherichia coli and in vitro. uniquely, the regulator responded specifically to zn(ii), cd(ii), and co(ii) and was named zccr. gel shift assays confirmed that zccr binds to an adjacent divergent promoter possessing an elongated spacer region of 19bp between the -10 and -35 elements, and that zn(ii), co(ii), and cd(ii) reduced the protein affinity for dna. site-directed mutagenesis of four cystein ... | 2003 | 12646225 |
| role of antibodies in immunity to bordetella infections. | the persistence of bordetella pertussis and b. parapertussis within vaccinated populations and the reemergence of associated disease highlight the need to better understand protective immunity. the present study examined host immunity to bordetellae and addressed potential concerns about the mouse model by using a comparative approach including the closely related mouse pathogen b. bronchiseptica. as previously observed with b. pertussis, all three organisms persisted throughout the respiratory ... | 2003 | 12654784 |
| expression of clostridium difficile toxins a and b and their sigma factor tcdd is controlled by temperature. | growth temperature was found to control the expression of toxins a and b in clostridium difficile vpi 10463, with a maximum at 37 degrees c and low levels at 22 and 42 degrees c in both peptone yeast (py) and defined media. the up-regulation of toxin a and b mrna and protein levels upon temperature upshift from 22 to 37 degrees c followed the same kinetics, showing that temperature control occurred at the level of transcription. experiments with clostridium perfringens using gusa as a reporter g ... | 2003 | 12654792 |
| protective efficacy of anti-helicobacter pylori immunity following systemic immunization of neonatal mice. | helicobacter pylori infection of the gastric mucosa is a significant cause of morbidity and mortality because of its etiologic role in symptomatic gastritis, peptic ulcer disease, and gastric adenocarcinoma. infection occurs in young children; therefore, a prophylactic vaccine would have to be administered within the first year of life, a period thought to be immunologically privileged. we investigated vaccine formulations administered by different routes to confer protective anti-h. pylori immu ... | 2003 | 12654796 |
| novel virulence-associated type ii secretion system unique to high-pathogenicity yersinia enterocolitica. | yersinia enterocolitica strains comprise an important group of bacterial enteropathogens that cause a broad range of gastrointestinal syndromes. three groups are distinguishable within this bacterial species, namely, the nonpathogenic group (biotype 1a strains), the low-pathogenicity, non-mouse-lethal group (biotypes 2 to 5), and the high-pathogenicity, mouse-lethal group (biotype 1b). to date, the presence of the high-pathogenicity island (hpi), a chromosomal locus that encodes the yersiniabact ... | 2003 | 12654803 |
| mucosal immunization with a genetically engineered pertussis toxin s1 fragment-cholera toxin subunit b chimeric protein. | a chimeric protein consisting of a divalent pertussis toxin (pt) s1 fragment linked to the cholera toxin (ctx) a(2)b fragment was constructed. the chimera induced a mucosal immunoglobulin a (iga) and a serum igg immune response to pt and ctxb in balb/c mice following intranasal immunization. the immune sera neutralized pt in vitro. in the mouse model of bordetella pertussis respiratory infection, the chimera-immunized animals showed a significant reduction in bacterial lung counts (p = 0.01) fro ... | 2003 | 12654855 |
| permeabilization in a cerebral endothelial barrier model by pertussis toxin involves the pkc effector pathway and is abolished by elevated levels of camp. | respiratory tract infections caused by bordetella pertussis are occasionally accompanied by severe neurologic disorders and encephalopathies. for these sequelae to occur the integrity of cerebral barriers needs to be compromised. the influence of pertussis toxin, a decisive virulence factor in the pathogenesis of pertussis disease, on barrier integrity was investigated in model systems for blood-liquor (epithelial) and blood-brain (endothelial) barriers. while pertussis toxin did not influence t ... | 2003 | 12665564 |
| activation of the complement cascade by bordetella pertussis. | bordetella pertussis must survive the defenses of the human respiratory tract including the complement system. the brka (bordetella resistance to killing) protein prevents killing by the antibody-dependent classical pathway. in this study, the ability of b. pertussis to activate the human complement cascade by other pathways was examined. b. pertussis was not killed in serum depleted of c2, however serum depleted for factor b killed b. pertussis as efficiently as intact serum, suggesting complem ... | 2003 | 12670691 |
| bugging the bugs. genomics and proteomics may provide new ways of making the lives of bacteria more miserable. | 2003 | 12671675 | |
| evaluation of the toxicity of recombinant bordetella pertussis adenylate cyclase toxin preparations. | 2002 | 12678231 | |
| advanced process monitoring: the ultimate alternative. | parametric release has the possibility to be the ultimate alternative for the use of animal tests. it involves the release of a product using evaluation of tightly controlled physical parameters, without the performance of a test on the final product. this practice is strengthened if there is a lack of validity for the final test to indicate quality or consistency. that suggests that in-process monitoring should replace the finished product controls when among others, batch-to-batch consistency ... | 2002 | 12678250 |
| quantitative detection of moraxella catarrhalis in nasopharyngeal secretions by real-time pcr. | the recognition of moraxella catarrhalis as an important cause of respiratory tract infections has been protracted, mainly because it is a frequent commensal organism of the upper respiratory tract and the diagnostic sensitivity of blood or pleural fluid culture is low. given that the amount of m. catarrhalis bacteria in the upper respiratory tract may change during infection, quantification of these bacteria in nasopharyngeal secretions (npss) by real-time pcr may offer a suitable diagnostic ap ... | 2003 | 12682118 |
| comparison of ribotyping and restriction enzyme analysis for inter- and intraspecies discrimination of bordetella avium and bordetella hinzii. | bordetella avium is an avian respiratory disease pathogen responsible for substantial economic losses to the turkey industry. the inability to distinguish isolates has hampered outbreak investigations and prevents a complete understanding of transmission mechanisms. isolates of bordetella hinzii, often referred to as b. avium-like or as alcaligenes faecalis type ii prior to 1995, have also been acquired from the respiratory tracts of diseased poultry but are not believed to be pathogenic for bir ... | 2003 | 12682138 |
| h5-ht(1b) receptor-mediated constitutive galphai3-protein activation in stably transfected chinese hamster ovary cells: an antibody capture assay reveals protean efficacy of 5-ht. | 1. serotonin 5-ht(1b) receptors couple to g-proteins of the gi/o family. however, their activation of specific g-protein subtypes is poorly characterised. using an innovative antibody capture/guanosine-5'-0-(3-[(35)s]thio)-triphosphate ([(35)s]gtpgammas) binding strategy, we characterised galpha(i3) subunit activation by h5-ht(1b) receptors stably expressed in chinese hamster ovary (cho) cells. 2. the agonists, 5-ht, alniditan and bms181,101, stimulated galpha(i3), whereas methiothepin and sb224 ... | 2003 | 12684263 |
| eotaxin-3 is a natural antagonist for ccr2 and exerts a repulsive effect on human monocytes. | eotaxin-3 (ccl26) belongs to the group of cc chemokines that attract eosinophils, basophils, and th2 lymphocytes. like eotaxin (ccl11) and eotaxin-2 (ccl24), eotaxin-3 mediates its activity through ccr3. here we show that eotaxin-3 also binds to ccr2 on monocytes and ccr2-transfected cells. in contrast to monocyte chemotactic protein 1 (mcp-1; ccl2), eotaxin-3 does not trigger intracellular calcium mobilization, enzyme release, or phosphorylation of the mitogen-activated protein (map) kinase erk ... | 2003 | 12689946 |
| bordetella pertussis protein pertactin induces type-specific antibodies: one possible explanation for the emergence of antigenic variants? | divergence has been found between bordetella pertussis vaccine strains and circulating strains. polymorphism in pertactin (prn) is essentially limited to region 1, which is made up of repeats. today, the 3 most prevalent prn variants are prn1-3. vaccine strains produce prn1, whereas prn2 is the predominant type found in circulating strains. we investigated how variation in region 1 affects the production of human serum antibodies. individuals infected by prn2 strains had significantly fewer anti ... | 2003 | 12695998 |
| simple sequence repeats and compositional bias in the bipartite ralstonia solanacearum gmi1000 genome. | ralstonia solanacearum is an important plant pathogen. the genome of r. solananearum gmi1000 is organised into two replicons (a 3.7-mb chromosome and a 2.1-mb megaplasmid) and this bipartite genome structure is characteristic for most r. solanacearum strains. to determine whether the megaplasmid was acquired via recent horizontal gene transfer or is part of an ancestral single chromosome, we compared the abundance, distribution and composition of simple sequence repeats (ssrs) between both repli ... | 2003 | 12697060 |
| agrobacterium tumefaciens virb6 protein participates in formation of virb7 and virb9 complexes required for type iv secretion. | this study characterized the contribution of agrobacterium tumefaciens virb6, a polytopic inner membrane protein, to the formation of outer membrane virb7 lipoprotein and virb9 protein multimers required for type iv secretion. virb7 assembles as a disulfide cross-linked homodimer that associates with the t pilus and a virb7-virb9 heterodimer that stabilizes other virb proteins during biogenesis of the secretion machine. two presumptive virb protein complexes, composed of virb6, virb7, and virb9 ... | 2003 | 12700266 |
| delayed immunisation and risk of pertussis in infants: unmatched case-control study. | 2003 | 12702617 | |
| pertussis immunisation. | 2003 | 12702618 | |
| mutations in the lspa1 and lspa2 genes of haemophilus ducreyi affect the virulence of this pathogen in an animal model system. | haemophilus ducreyi 35000hp contains two genes, lspa1 and lspa2, whose predicted protein products have molecular weights of 456,000 and 543,000, respectively (c. k. ward, s. r. lumbley, j. l. latimer, l. d. cope, and e. j. hansen, j. bacteriol. 180:6013-6022, 1998). we have constructed three h. ducreyi 35000hp mutants containing antibiotic resistance cartridges in one or both of the lspa1 and lspa2 open reading frames. western blot analysis using lspa1- and lspa2-specific monoclonal antibodies i ... | 2003 | 12704119 |
| unexpected similarities between bordetella avium and other pathogenic bordetellae. | bordetella avium causes an upper respiratory tract disease (bordetellosis) in avian species. commercially raised turkeys are particularly susceptible. like other pathogenic members of the genus bordetella (b. pertussis and b. bronchiseptica) that infect mammals, b. avium binds preferentially to ciliated tracheal epithelial cells and produces similar signs of disease. these similarities prompted us to study bordetellosis in turkeys as a possible nonmammalian model for whooping cough, the exclusiv ... | 2003 | 12704133 |
| declining pertussis incidence in sweden following the introduction of acellular pertussis vaccine. | acellular pertussis vaccines were introduced nation-wide in sweden in 1996, 17 years after the withdrawal of whole-cell pertussis vaccine from the childhood immunisation schedule. we report national data on age specific incidence of culture-confirmed bordetella pertussis for 1986-2000, and clinical follow-up for 3 years (october 1997-september 2000) in children born in 1996-2000 and from children born in 1993-1994 who had participated in a trial of pertussis vaccines. the annual incidence of cul ... | 2003 | 12706691 |
| bordetella pertussis and bordetella parapertussis infection in an austrian pediatric outpatient clinic. | to determine the proportion of children with an acute cough and laboratory evidence of bordetella pertussis (b. pertussis) and b. parapertussis infection. | 2002 | 12708090 |
| peptide-based analysis of the amino acid sequence important to the immunoregulatory function of trypanosoma cruzi tc52 virulence factor. | the intracellular protozoan parasite trypanosoma cruzi is the aetiological agent of chagas' disease. we have previously identified a t. cruzi-released protein called tc52, which is crucial for parasite survival and virulence. in the present study, we attempted to define the tc52 epitope(s) responsible for its immunoregulatory function. a naturally occurring major peptide fragment of molecular mass 28 kda (tc28k) was identified, which was localized in the c-terminal portion of tc52 and was inhibi ... | 2003 | 12709028 |
| efficient display of two enzymes on filamentous phage using an improved signal sequence. | directed protein-evolution strategies generally make use of a link between a protein and the encoding dna. in phage-display technology, this link is provided by fusion of the protein with a coat protein that is incorporated into the phage particle containing the dna. optimization of this link can be achieved by adjusting the signal sequence of the fusion. in a previous study, directed evolution of signal sequences for optimal display of the taq dna polymerase i stoffel fragment on phage yielded ... | 2003 | 12721491 |
| phagocytosis of bordetella pertussis incubated with convalescent serum. | convalescent serum samples were examined for the ability to promote phagocytosis of bordetella pertussis by human neutrophils. one sample promoted phagocytosis, and 11 of the 51 samples caused a statistically significant reduction in phagocytosis, compared with that of bacteria not incubated with serum. phagocytosis was influenced by interactions between antibodies that promoted phagocytosis and antibodies that inhibited phagocytosis. adenylate cyclase toxin (act) has been shown to block phagocy ... | 2003 | 12721945 |
| functional aspects of protein mono-adp-ribosylation. | mono-adp-ribosylation is the enzymatic transfer of adp-ribose from nad(+) to acceptor proteins. it is catalysed by cellular adp-ribosyltransferases and certain bacterial toxins. there are two subclasses of cellular enzymes: the ectoenzymes that modify targets such as integrins, defensin and other cell surface molecules; and the intracellular enzymes that act on proteins involved in cell signalling and metabolism, such as the beta-subunit of heterotrimeric g proteins, grp78/bip and elongation fac ... | 2003 | 12727863 |
| evaluation of a new cellulose sponge-tipped swab for microbiological sampling: a laboratory and clinical investigation. | a new type of swab (cellswab; cellomeda, turku, finland), utilizing a highly absorbent cellulose viscose sponge material, was compared to some traditional swabs. the survival of 14 aerobic and 10 anaerobic and microaerophilic bacterial species in the cellswab, two commercial swab transport systems (copan, brescia, italy, and orion diagnostica, espoo, finland), and one dacron swab (technical service consultants ltd. [tsc], heywood, united kingdom) was evaluated. bacteria were suspended in broth, ... | 2003 | 12734223 |
| expression, purification and characterization of ricin vectors used for exogenous antigen delivery into the mhc class i presentation pathway. | disarmed versions of the cytotoxin ricin can deliver fused peptides into target cells leading to mhc class i-restricted antigen presentation [smith et al. j immunol 2002; 169:99-107]. the ricin delivery vector must contain an attenuated catalytic domain to prevent target cell death, and the fused peptide epitope must remain intact for delivery and functional loading to mhc class i molecules. expression in e. coli and purification by cation exchange chromatography of the fusion protein is describ ... | 2003 | 12734560 |
| respiratory disease in kennelled dogs: serological responses to bordetella bronchiseptica lipopolysaccharide do not correlate with bacterial isolation or clinical respiratory symptoms. | the role of bordetella bronchiseptica in a natural outbreak of canine infectious respiratory disease was investigated both by culture and serological analysis. b. bronchiseptica was found in the lungs of a large proportion of clinically healthy dogs and in a greater proportion of dogs with respiratory disease. using a lipopolysaccharide (lps) antigen-based enzyme-linked immunosorbent assay, we analyzed the serological responses of a large number of dogs. dogs with high antibody levels showed no ... | 2003 | 12738630 |
| [diagnostic image (136). a boy with coughing fits and subconjunctival hemorrhage. subconjunctival hemorrhage secondary to whooping cough]. | an 8-year-old boy suffered from persisting coughing fits for two weeks and subconjunctival haemorrhage since one week. in paired sera serological proof of infection with bordetella pertussis was found. | 2003 | 12741169 |
| seroprevalence of bordetella pertussis antibodies in flanders (belgium). | to determine age-dependent pertussis immune response in the flemish population, antibody levels to pertactin (prn), pertussis toxin (pt) and filamentous hemagglutinin (fha) were measured in serum samples from 1622 healthy 1-100-year-old subjects. for anti-prn and anti-pt antibodies, peaks in gmts were seen in infancy and again in the 10-15-year age group. after age of 20 years, anti-prn gmt declined rapidly over a decade, followed by a slower decline. anti-fha gmt tended to rather increase progr ... | 2003 | 12744872 |
| a marked decrease in l-selectin expression by leucocytes in infants with bordetella pertussis infection: leucocytosis explained? | infants with bordetella pertussis infection (whooping cough) have an unexplained lymphocytosis and leucocytosis characterized by an increase in small lymphocytes with convoluted and cleaved nuclei. to characterize these cells immunophenotyping using multiparameter flow cytometry was performed on leucocytes from a group of 11 infants aged 3-6 months with proven pertussis and from uninfected control subjects. | 2003 | 12753529 |
| induction of autoimmune valvulitis in lewis rats following immunization with peptides from the conserved region of group a streptococcal m protein. | rheumatic heart disease (rhd) is considered to be an autoimmune disorder mediated by group a streptococcal (gas) m protein-specific t cells and antibodies that cross-react with cardiac antigens and epitopes of the gas m protein. in this study, lewis rats were immunized with a pool of overlapping peptides spanning the conserved region of the gas m protein in complete freund's adjuvant, followed by immunization with bordetella pertussis. controls received adjuvants alone. spleen-derived lymphocyte ... | 2003 | 12753806 |
| functional subsets of the virb type iv transport complex proteins involved in the capacity of agrobacterium tumefaciens to serve as a recipient in virb-mediated conjugal transfer of plasmid rsf1010. | the virb-encoded type iv transport complex of agrobacterium tumefaciens mediates the transfer of dna and proteins into plant cells, as well as the conjugal transfer of incq plasmids, such as rsf1010, between agrobacterium strains. while several studies have indicated that there are physical interactions among the 11 virb proteins, the functional significance of the interactions has been difficult to establish since all of the proteins are required for substrate transfer. our previous studies, ho ... | 2003 | 12754223 |
| antigen-capturing cells can masquerade as memory b cells. | as well as classically defined switched immunoglobulin isotype-expressing b cells, memory b cells are now thought to include igm-expressing cells and memory cells that lack b cell lineage markers, such as b220 or cd19. we set out to compare the relative importance of memory b cell subsets with an established flow cytometry method to identify antigen-specific cells. after immunization with pe, we could detect b220+ and, as reported previously, b220- antigen-binding cells (mcheyzer-williams, l.j., ... | 2003 | 12756262 |
| progress in the diagnosis, prevention, and treatment of pertussis. | pertussis ("whooping cough"), caused by the gram- negative pleomorphic bacillus bordetella pertussis, is a highly contagious, potentially life-threatening respiratory tract illness that has re-emerged worldwide as a cause of substantial morbidity and mortality in infants, children, and adolescents, even in countries with high vaccination rates. waning immunity after immunization during childhood has been associated with a growing pool of susceptible adolescents and adults who are capable of tran ... | 2003 | 12760818 |
| comparison of bipa alleles within and across bordetella species. | the bordetella bvgas signal transduction system controls the expression of at least three phenotypic phases, the bvg(+) or virulent phase, the bvg(-) or avirulent phase, and the bvg(i) or bvg intermediate phase, which has been hypothesized to be important for transmission. bipa, the first identified bvg(i)-phase gene, encodes a protein with similarity to the well-characterized bacterial adhesins intimin and invasin. proteins encoded by the bipa genes present in bordetella pertussis tohama i and ... | 2003 | 12761081 |
| nonpathogenic escherichia coli can contribute to the production of shiga toxin. | the food-borne pathogen, escherichia coli o157:h7, has been associated with gastrointestinal disease and the life-threatening sequela hemolytic uremic syndrome. the genes for the virulence factor, shiga toxin 2 (stx2), in e. coli o157:h7 are encoded on a temperate bacteriophage under the regulation of the late gene promoter. induction of the phage lytic cycle is required for toxin synthesis and release. we investigated the hypothesis that nonpathogenic e. coli could amplify stx2 production if in ... | 2003 | 12761088 |
| fishing new proteins in the twilight zone of genomes: the test case of outer membrane proteins in escherichia coli k12, escherichia coli o157:h7, and other gram-negative bacteria. | we address the problem of clustering the whole protein content of genomes into three different categories-globular, all-alpha, and all-beta membrane proteins-with the aim of fishing new membrane proteins in the pool of nonannotated proteins (twilight zone). the focus is then mainly on outer membrane proteins. this is performed by using an integrated suite of programs (hunter) specifically developed for predicting the occurrence of signal peptides in proteins of gram-negative bacteria and the top ... | 2003 | 12761386 |
| molecular typing of bordetella pertussis isolates recovered from belgian children and their household members. | recently, a moderate increase in the prevalence of pertussis, possibly contracted from adults, has been observed among unvaccinated children. during a 3-year period, we prospectively enrolled 93 index patients with a polymerase chain reaction (pcr) and/or culture result positive for bordetella pertussis. among 63 household contacts of 28 index patients, pcr and culture for b. pertussis identified 25 b. pertussis-positive persons. nineteen of 25 b. pertussis-positive household contacts were asymp ... | 2003 | 12766833 |
| differential modulation of bordetella pertussis virulence genes as evidenced by dna microarray analysis. | the production of most factors involved in bordetella pertussis virulence is controlled by a two-component regulatory system termed bvga/s. in the bvg+ phase virulence-activated genes (vags) are expressed, and virulence-repressed genes (vrgs) are down-regulated. the expression of these genes can also be modulated by mgso(4) or nicotinic acid. in this study we used microarrays to analyse the influence of bvga/s or modulation on the expression of nearly 200 selected genes. with the exception of on ... | 2003 | 12768411 |
| getting a grip on non-native proteins. | it is an underappreciated fact that non-native polypeptides are prevalent in the cellular environment. native proteins have the folded structure, assembled state and cellular localization required for activity. by contrast, non-native proteins lack function and are particularly prone to aggregation because hydrophobic residues that are normally buried are exposed on their surfaces. these unstable entities include polypeptides that are undergoing synthesis, transport to and translocation across m ... | 2003 | 12776175 |
| absence of cd4+cd25+ regulatory t cells is associated with a loss of regulation leading to increased pathology in helicobacter pylori-infected mice. | helicobacter pylori induces symptomatic chronic gastritis in a subpopulation of infected individuals. the mechanism(s) determining the development and severity of pathology leading to symptoms are not fully understood. in a mouse model of h. pylori infection we analysed the influence of immunoregulatory cd4+cd25+ t cells on h. pylori colonization and gastritis. athymic c57bl/6 nu/nu mice were reconstituted with (a) lymph node (ln) cells (b) ln cells depleted of cd25+ t cells (cd25(-) ln) or (c) ... | 2003 | 12780684 |
| detection and discrimination of b pertussis and b holmesii by real-time pcr targeting is481 using a beacon probe and probe-target melting analysis. | a beacon probe was designed to detect one of the two documented single nucleotide changes in is481 target allele of bordetella holmesii genome as compared to bordetella pertussis. pcr amplified product targeting a region of is481 in presence of the probe was subjected to a post-pcr hybridization and melting cycle. hybrid of the probe with b. pertussis specific target had a different thermal stability than that with allele having the single nucleotide change in b. holmesii. the melting of b. pert ... | 2003 | 12788030 |
| histone-like proteins of the dinoflagellate crypthecodinium cohnii have homologies to bacterial dna-binding proteins. | the dinoflagellates have very large genomes encoded in permanently condensed and histoneless chromosomes. sequence alignment identified significant similarity between the dinoflagellate chromosomal histone-like proteins of crypthecodinium cohnii (hccs) and the bacterial dna-binding and the eukaryotic histone h1 proteins. phylogenetic analysis also supports the origin of the hccs from histone-like proteins of bacteria. | 2003 | 12796310 |
| the cox-2-specific inhibitor celecoxib inhibits adenylyl cyclase. | nonsteroidal anti-inflammatory drugs (nsaids) are well-known causes of acute renal insufficiency and gastropathy in patients with chronic inflammatory diseases. this action is presumed to result from nonselective inhibition of both constitutive and inducible forms of prostaglandin h synthases, also known as the cyclooxygenase enzymes (i.e., cox-1 amd cox-2). celecoxib (celebrex) is a cox-2 enzyme inhibitor and has emerged as a preferred therapeutic agent for the treatment of rheumatoid arthritis ... | 2003 | 12797547 |
| cell-surface bound pertussis toxin induces polyclonal t cell responses with high levels of interferon-gamma in the absence of interleukin-12. | pertussis toxin (ptx), an exotoxin produced by bordetella pertussis, has long been used as a mucosal adjuvant. we examined the t cell stimulatory properties of ptx in order to dissect its mechanisms of adjuvanticity. ptx or the b-oligomer of ptx (ptxb) failed to activate purified murine cd4+ or cd8+ t cells, as measured by a lack of proliferation or expression of early t cell activation markers. however, these t cells proliferated extensively in response to the toxin in the presence of syngeneic ... | 2003 | 12811846 |
| sites of interaction between the feca and fecr signal transduction proteins of ferric citrate transport in escherichia coli k-12. | transcription of the fecabcde ferric citrate transport genes of escherichia coli k-12 is initiated by a signaling cascade from the cell surface into the cytoplasm. fecr receives the signal in the periplasm from the outer membrane protein feca loaded with ferric citrate, transmits the signal across the cytoplasmic membrane, and converts feci in the cytoplasm to an active sigma factor. in this study, it was shown through the use of a bacterial two-hybrid system that, in the periplasm, the c-termin ... | 2003 | 12813067 |
| pertussis in adults. a study in an italian population with chronic cough. | pertussis is commonly regarded as a disease of children but a number of clinical studies show that quite often it occurs in older children and adults, too. the purpose of the present study was to determine the prevalence of bordetella pertussis infection in an italian population of adults with persistent non-productive cough. | 2002 | 12814036 |
| functional similarities between the icm/dot pathogenesis systems of coxiella burnetii and legionella pneumophila. | coxiella burnetii, the etiological agent of q fever, is an obligate intracellular pathogen, whereas legionella pneumophila, the causative agent of legionnaires' disease, is a facultative intracellular pathogen. during infection of humans both of these pathogens multiply in alveolar macrophages inside a closed phagosome. l. pneumophila intracellular multiplication was shown to be dependent on the icm/dot system, which probably encodes a type iv-related translocation apparatus. recently, genes hom ... | 2003 | 12819052 |
| the c-terminal domain of salmonella enterica serovar typhimurium ompa is an immunodominant antigen in mice but appears to be only partially exposed on the bacterial cell surface. | we examined the way the major outer membrane protein ompa of salmonella enterica serovar typhimurium is recognized by the mouse immune system, by raising a panel of 12 monoclonal antibodies (mabs) against this protein. interaction between ompa and these mabs is competitively inhibited with several-hundredfold dilutions of mouse polyclonal sera obtained by immunization with live or heat-killed whole cells, suggesting that ompa is one of the immunodominant antigens of serovar typhimurium. all of t ... | 2003 | 12819080 |
| bordetella bronchiseptica persists in the nasal cavities of mice and triggers early delivery of dendritic cells in the lymph nodes draining the lower and upper respiratory tract. | early after the intranasal instillation of bordetella bronchiseptica into mice, not only are mature dendritic leukocytes recovered from lung parenchyma and bronchoalveolar lavage fluid but their numbers are also increased in the mediastinal lymph nodes and the nasal mucosa-associated lymphoid tissue. later during the infectious process, the bacteria persist mainly in the nasal cavity. | 2003 | 12819105 |
| a mammalian peptidoglycan recognition protein with n-acetylmuramoyl-l-alanine amidase activity. | the family of peptidoglycan recognition proteins (pgrps) is conserved from insects to mammals. recently, drosophila pgrp-sc1b was demonstrated to be an n-acetylmuramoyl-l-alanine amidase (namlaa), an enzyme that cleaves the lactylamide bond between muramic acid and the peptide chain in peptidoglycan (pgn). we now show an m x mpgrp-l mrna to be expressed in the liver. the recombinant m x mpgrp-l protein has namlaa activity and degrades pgn from both escherichia coli and staphylococcus aureus; how ... | 2003 | 12821140 |
| oral colonization and immune responses to streptococcus gordonii: potential use as a vector to induce antibodies against respiratory pathogens. | mucosal immunization should be an excellent method of preventing respiratory infections because the local immunoglobulin a antibodies can neutralize the invading pathogens at the site of entry. because streptococcus gordonii, a normal inhabitant of the human oral cavity, can naturally elicit a mucosal immune response, it has been a prime candidate for investigations as a live oral vaccine vector for immunization against respiratory infections. | 2003 | 12821813 |
| functional coupling between heterologously expressed dopamine d(2) receptors and kcnq channels. | activation of kcnq potassium channels by stimulation of co-expressed dopamine d(2) receptors was studied electrophysiologically in xenopus laevis oocytes and in mammalian cells. to address the specificity of the interaction between d(2)-like receptors and kcnq channels, combinations of kcnq1-5 channels and d(2)-like receptors (d(2l), d(3), and d(4)) were investigated in xenopus oocytes. activation of either receptor with the selective d(2)-like receptor agonist quinpirole (100 nm) stimulated all ... | 2003 | 12827359 |
| surface anchoring of bacterial subtilisin important for maturation function. | many extracytoplasmic proteins undergo proteolytic processing during secretion, which is essential to their maturation. these post-translational modifications are carried out by specific enzymes whose subcellular localization is important for function. we have described a maturation subtilisin in gram-negative bordetella pertussis, the autotransporter sphb1. sphb1 catalyses the maturation of the precursor of the adhesin filamentous haemagglutinin (fha) at the bacterial surface, in addition to th ... | 2003 | 12828647 |
| use of bordetella bronchiseptica and bordetella pertussis as live vaccines and vectors for heterologous antigens. | bordetella pertussis and bordetella bronchiseptica are respiratory pathogens of humans and animals respectively. unlike many bacteria, they are able to efficiently colonise healthy ciliated respiratory mucosa. this characteristic of bordetella spp. can potentially be exploited to develop efficient live vaccines and vectors for delivery of heterologous antigens to the respiratory tract. here we review the progress in this area. | 2003 | 12832115 |
| channel formation in model membranes by the adenylate cyclase toxin of bordetella pertussis: effect of calcium. | calmodulin-dependent adenylate cyclase toxin (act or cyaa) of bordetella pertussis requires calcium ions for target cell binding, formation of hemolytic channels, and delivery of its enzyme component into cells. we examined the effect of calcium and calmodulin on toxin interaction with planar lipid bilayers. while calmodulin binding did not affect the properties of cyaa channels, addition of calcium ions and toxin to the same side of the membrane caused a steep increase of the channel-forming ca ... | 2003 | 12834359 |
| homotrimeric, beta-stranded viral adhesins and tail proteins. | 2003 | 12837775 | |
| immunogenicity and safety in infants of a dtwphib full liquid vaccine. | combining paediatric vaccines is a rational solution to reduce the number of injections during a single clinical visit, to maintain parents' compliance and to extend vaccine coverage. different diphtheria, tetanus and whole cell pertussis (dtwp)-containing combination vaccines are licensed and used world-wide. this study assessed the immunogenicity and safety in infants of a combined diphtheria-tetanus-whole cell pertussis-haemophilus influenzae type b-crm197 conjugate full liquid vaccine. | 2003 | 12839281 |
| freshwater bacteria can methylate selenium through the thiopurine methyltransferase pathway. | involvement of the bacterial thiopurine methyltransferase (btpmt) in natural selenium methylation by freshwater was investigated. a freshwater environment that had no known selenium contamination but exhibited reproducible emission of dimethyl selenide (dmse) or dimethyl diselenide (dmdse) when it was supplemented with an organic form of selenium [(methyl)selenocysteine] or an inorganic form of selenium (sodium selenite) was used. the distribution of the btpmt gene (tpm) in the microflora was st ... | 2003 | 12839745 |
| strain variation among bordetella pertussis isolates from québec and alberta provinces of canada from 1985 to 1994. | pulsed-field gel electrophoresis and gene typing were able to differentiate among 3,597 bordetella pertussis isolates circulating in alberta and québec provinces, canada, from 1985 to 1994 and distinguish them from the strains used in vaccine production. this study provides a baseline for continued surveillance of prevalent and emerging strains of b. pertussis in canada. | 2003 | 12843089 |
| t-cell immune response assessment as a complement to serology and intranasal protection assays in determining the protective immunity induced by acellular pertussis vaccines in mice. | the relative value of antibodies and/or t-cell immune responses to bordetella pertussis antigens in the immunity induced by acellular pertussis (ap) vaccines is still an open issue, probably due to the incomplete knowledge on the mechanisms of protective immunity to pertussis. the relevance of t-cell immune responses in protection from pertussis has been demonstrated in murine and human models of infection; thus, in this study, the ability of different vaccine preparations of three component (pe ... | 2003 | 12853397 |
| human immunodeficiency virus (hiv) type 1 transframe protein can restore activity to a dimerization-deficient hiv protease variant. | the protease (pr) from human immunodeficiency virus (hiv) is essential for viral replication: this aspartyl protease, active only as a dimer, is responsible for cleavage of the viral polyprotein precursors (gag and gag-pol), to release the functional mature proteins. in this work, we have studied the structure-function relationships of the hiv pr by combining a genetic test to detect proteolytic activity in escherichia coli and a bacterial two-hybrid assay to analyze pr dimerization. we showed t ... | 2003 | 12857890 |
| association of bordetella pertussis with host immune cells in the mouse lung. | mouse models are frequently used to study immunity and pathogenesis to bordetella pertussis infection. to improve the understanding of the mouse infection model, the influx of host cells and b. pertussis localisation in the lungs were evaluated. furthermore, the roles of filamentous hemagglutinin (fha) and fimbriae (fim) in these processes were determined. b. pertussis infection stimulated the recruitment of polymorphonuclear granulocytes (pmn), alveolar macrophages, and lymphocytes. as determin ... | 2003 | 12860455 |
| description of a multiplex bordetella pertussis and bordetella parapertussis lightcycler pcr assay with inhibition control. | while culture for bordetella species is highly specific, sensitivity is extremely variable due to patient age, immunization status, antibiotic treatment, and specimen transport conditions. we evaluated a real-time multiplex pcr assay as an alternative to culture for the detection and differentiation of bordetella pertussis and bordetella parapertussis. the pcr conditions allowed the simultaneous detection of one b. pertussis organism and five b. parapertussis organisms per reaction. an inhibitio ... | 2003 | 12867094 |
| characterization of a new lexa binding motif in the marine magnetotactic bacterium strain mc-1. | mc-1 is a marine, magnetotactic bacterium that is phylogenetically associated with the alpha subclass of the proteobacteria and is the first and only magnetotactic coccus isolated in pure culture to date. by using a tblastn search, a lexa gene was identified in the published genome of mc-1; it was subsequently cloned, and the protein was purified to >90% purity. results from reverse transcription-pcr analysis revealed that the mc-1 lexa gene comprises a single transcriptional unit with two open ... | 2003 | 12867456 |
| reciprocal conditioning: t cells as regulators of dendritic cell function. | 2003 | 12871212 | |
| caspase 1 involvement in human monocyte lysis induced by actinobacillus actinomycetemcomitans leukotoxin. | actinobacillus actinomycetemcomitans, an oral bacterium implicated in the etiology of periodontal diseases, produces a leukotoxin that selectively lyses primate neutrophils and monocytes, the major populations of defense cells in the periodontium. though lysis requires expression of the receptor lymphocyte function-associated molecule 1 (lfa-1) on the cell surface, not all lfa-1-expressing leukocyte populations are equally susceptible to the toxin. in this study, the susceptibility of human leuk ... | 2003 | 12874324 |
| clinical and epidemiological picture of b pertussis and b parapertussis infections after introduction of acellular pertussis vaccines. | to investigate the clinical picture and frequency of bordetella pertussis and b parapertussis infections after introduction of acellular pertussis (acp) vaccines in germany. | 2003 | 12876162 |
| interaction of bordetella pertussis adenylate cyclase with cd11b/cd18: role of toxin acylation and identification of the main integrin interaction domain. | adenylate cyclase toxin (cyaa) is one of the major virulence factors produced by bordetella pertussis, the whooping cough agent. cyaa belongs to the repeat in toxin protein family and requires a post-translational fatty acylation to form cation-selective channels in target cell membranes and to penetrate into cytosol. we have demonstrated recently that cyaa uses the alphambeta2 integrin (cd11b/cd18) as a specific cellular receptor. here we show that the acylation of cyaa is required for a produc ... | 2003 | 12885782 |
| [morphological alterations in animal organs after the injection of acellular pertussis vaccine with immunomodulator]. | toxic properties of acellular pertussis vaccine (apv) and morphological changes in white mice in response to intramuscular injection of apv (without or with immunomodulator glucosaminylmuramyl dipeptide-gmdp) were under study. apv used in these experiments was developed at the mechnikov research institute for vaccines and sera (the russian acad. med. sci.) on the basis of bordetella pertussis cultures in synthetic fluid culture media. in experiments on acute and chronic toxicity of apv (without ... | 2003 | 12886628 |
| vir90, a virulence-activated gene coding for a bordetella pertussis iron-regulated outer membrane protein. | bordetella pertussis undergoes phenotypic changes modulated by the bvgas locus, which regulates the expression of many genes related to virulence and immunogenicity. we previously reported the n-terminal sequence of a 90 kda bvg-regulated outer membrane protein (omp) of b. pertussis (swiss-prot accession no. p81549), a novel potential virulence factor that we named vir90. the open reading frames (orfs) which potentially code for vir90 in b. pertussis, b. parapertussis and b. bronchiseptica were ... | 2003 | 12892851 |
| single-nucleotide-polymorphism mapping of the pseudomonas aeruginosa type iii secretion toxins for development of a diagnostic multiplex pcr system. | we mapped the coding single nucleotide polymorphisms in four toxin genes-exos, exot, exou, and exoy-of the pseudomonas aeruginosa type iii secretion system among several clinical isolates. we then used this information to design a multiplex pcr assay based on the simultaneous amplification of fragments of these genes. eight strains of known genotype were used to test our multiplex pcr method, which showed 100% sensitivity and specificity in this small sample size. this assay appears to be promis ... | 2003 | 12904350 |
| comparison of the bordetella pertussis and bordetella parapertussis isolates circulating in saint petersburg between 1998 and 2000 with russian vaccine strains. | we analyzed the bordetella pertussis and bordetella parapertussis isolates circulating in saint petersburg that were collected between 1998 and 2000 and compared them with isolates collected 40 years ago and russian vaccine strains. the analysis involved serotyping, pulsed-field gel electrophoresis of chromosomal dna after digestion with xbai and spei, and sequencing of the ptxs1 and prn genes, which encode the s1 subunit of the pertussis toxin and the major adhesin pertactin, respectively. the ... | 2003 | 12904379 |
| rapid identification of bacteria from positive blood cultures by terminal restriction fragment length polymorphism profile analysis of the 16s rrna gene. | bacteremia results in significant morbidity and mortality, especially among patient populations that are immunocompromised. broad-spectrum antibiotics are administered to patients suspected to have bloodstream infections that are awaiting diagnosis that depends on blood culture analysis. significant delays in identification of pathogens can result, primarily due to the dependence on growth-based identification systems. to address these limitations, we took advantage of terminal restriction fragm ... | 2003 | 12904391 |
| comparative analysis of the genome sequences of bordetella pertussis, bordetella parapertussis and bordetella bronchiseptica. | bordetella pertussis, bordetella parapertussis and bordetella bronchiseptica are closely related gram-negative beta-proteobacteria that colonize the respiratory tracts of mammals. b. pertussis is a strict human pathogen of recent evolutionary origin and is the primary etiologic agent of whooping cough. b. parapertussis can also cause whooping cough, and b. bronchiseptica causes chronic respiratory infections in a wide range of animals. we sequenced the genomes of b. bronchiseptica rb50 (5,338,40 ... | 2003 | 12910271 |
| the use of microcalorimetry to characterize tetanus neurotoxin, pertussis toxin and filamentous haemagglutinin. | tetanus neurotoxin (tent), pertussis toxin (pt) and pertussis filamentous haemagglutinin (fha) are major virulence factors of clostridium tetani and bordetella pertussis, which are the causative agents of tetanus and whooping cough respectively. inactivated forms of these virulence factors are the protein components of vaccines against these diseases. here we report microcalorimetric studies to characterize these proteins. the microcalorimetric titration curves of tent with micelles of gangliosi ... | 2003 | 12911336 |
| repeated episodes of ozone inhalation amplifies the effects of allergen sensitization and inhalation on airway immune and structural development in rhesus monkeys. | twenty-four infant rhesus monkeys (30 days old) were exposed to 11 episodes of filtered air (fa), house dust mite allergen aerosol (hdma), ozone (o3), or hdma + o3 (5 days each followed by 9 days of fa). ozone was delivered for 8 h/day at 0.5 ppm. twelve of the monkeys were sensitized to house dust mite allergen (dermatophagoides farinae) at ages 14 and 28 days by subcutaneous inoculation (sq) of hdma in alum and intraperitoneal injection of heat-killed bordetella pertussis cells. sensitized mon ... | 2003 | 12915105 |
| the histone-like c-terminal extension in ribosomal protein s6 in aedes and anopheles mosquitoes is encoded within the distal portion of exon 3. | in eukaryotic cells, ribosomal protein s6 (rps6) is the major phosphorylated protein on the small ribosomal subunit. in the mosquitoes aedes aegypti and aedes albopictus, the cdna encoding rps6 contains 300 additional nucleotides, relative to the drosophila homolog. the additional sequence encodes a 100-amino acid, lysine-rich c-terminal extension of the rps6 protein with 42-49% identity to histone h1 proteins from the chicken and other multicellular organisms. using mass spectrometry we now sho ... | 2003 | 12915181 |
| low levels of antipertussis antibodies plus lack of history of pertussis correlate with susceptibility after household exposure to bordetella pertussis. | prospectively collected data in a swedish vaccine efficacy trial were used to investigate transmission of pertussis from small study infants to other household members. forty one percent (258/627) of the exposed persons with paired serology had laboratory confirmed pertussis. the majority of those with laboratory confirmed pertussis had less than 14 days of cough and many were asymptomatic. high susceptibility to symptomatic pertussis was found among persons with low initial igg antibody concent ... | 2003 | 12922081 |
| rtsa and rtsb coordinately regulate expression of the invasion and flagellar genes in salmonella enterica serovar typhimurium. | salmonella enterica serovar typhimurium encounters numerous host environments and defense mechanisms during the infection process. the bacterium responds by tightly regulating the expression of virulence genes. we identified two regulatory proteins, termed rtsa and rtsb, which are encoded in an operon located on an island integrated at trna(pheu) in s. enterica serovar typhimurium. rtsa belongs to the arac/xyls family of regulators, and rtsb is a helix-turn-helix dna binding protein. in a random ... | 2003 | 12923082 |
| cloning and expression of the gene for a novel protein from mycobacterium smegmatis with functional similarity to eukaryotic calmodulin. | a calmodulin-like protein (camlp) from mycobacterium smegmatis was purified to homogeneity and partially sequenced; these data were used to produce a full-length clone, whose dna sequence contained a 55-amino-acid open reading frame. m. smegmatis camlp, expressed in escherichia coli, exhibited properties characteristic of eukaryotic calmodulin: calcium-dependent stimulation of eukaryotic phosphodiesterase, which was inhibited by the calmodulin antagonist trifluoperazine, and reaction with anti-b ... | 2003 | 12923099 |
| comparison of real-time pcr and conventional hemi-nested pcr for the detection of bordetella pertussis in nasopharyngeal samples. | we directly compared a conventional hemi-nested pcr assay with a real-time (lightcycler) pcr assay for the detection of bordetella pertussis in nasopharyngeal samples. of the 152 samples tested, 49 (32%) were positive by first-round conventional pcr, 56 (37%) were positive by the hemi-nested pcr assay, and 39 (26%) were positive by the real-time assay. all samples testing positive with the real-time assay were also positive by the hemi-nested assay (both first- and second-round pcr), and all cul ... | 2003 | 12925123 |