Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| infections preceding early arthritis in southern sweden: a prospective population-based study. | to detect evidence of infections preceding early arthritis in southern sweden and to compare the clinical outcome of remission during a 6-month followup for patients with and without signs of prior infection. | 2003 | 12610801 |
| jlpa of campylobacter jejuni interacts with surface-exposed heat shock protein 90alpha and triggers signalling pathways leading to the activation of nf-kappab and p38 map kinase in epithelial cells. | campylobacter jejuni is a leading cause of acute bacterial gastroenteritis in humans. the mechanism by which c. jejuni interacts with host cells, however, is still poorly understood. our previous study has shown that the c. jejuni surface lipoprotein jlpa mediates adherence of the bacterium to epithelial cells. in this report, we demonstrated that jlpa interacts with hep-2 cell surface heat shock protein (hsp) 90alpha and initiates signalling pathways leading to activation of nf-kappab and p38 m ... | 2003 | 12614460 |
| gene expression profile of campylobacter jejuni in response to growth temperature variation. | the foodborne pathogen campylobacter jejuni is the primary causative agent of gastroenteritis in humans. in the present study a whole genome microarray of c. jejuni was constructed and validated. these dna microarrays were used to measure changes in transcription levels over time, as c. jejuni cells responded to a temperature increase from 37 to 42 degrees c. approximately 20% of the c. jejuni genes were significantly up- or downregulated over a 50-min period after the temperature increase. the ... | 2003 | 12618466 |
| expansion of human gammadelta t cells after in vitro stimulation with campylobacter jejuni. | campylobacter jejuni is currently the prime cause of food-borne bacterial gastro-enteritis. an important complication of c. jejuni enteritis is guillain-barré syndrome (gbs), an immune-mediated disorder of peripheral nerve tissue. because little is known about t cell reactivity to c. jejuni, we have analyzed the in vitro immune response of normal individuals against five isolates of c. jejuni representing five different serotypes. we found a preferential expansion of peripheral blood gammadelta ... | 2003 | 12618481 |
| molecular characterization of fluoroquinolone-resistant campylobacter spp. isolated from poultry. | campylobacteriosis, an infectious disease caused by campylobacter jejuni and campylobacter coli, is treated by fluoroquinolone antibiotics in clinical practices. however, use of these drugs in animal husbandry may select for fluoroquinolone-resistant campylobacters and, thereby, compromise the clinical treatment of infection. in this study, 21 fluoroquinolone-resistant campylobacters were isolated from poultry samples. morphological and biochemical characteristics indicated that 19 isolates were ... | 2003 | 12619802 |
| evolutionary history, structural features and biochemical diversity of the nlpc/p60 superfamily of enzymes. | peptidoglycan is hydrolyzed by a diverse set of enzymes during bacterial growth, development and cell division. the n1pc/p60 proteins define a family of cell-wall peptidases that are widely represented in various bacterial lineages. currently characterized members are known to hydrolyze d-gamma-glutamyl-meso-diaminopimelate or n-acetylmuramate-l-alanine linkages. | 2003 | 12620121 |
| lateral gene transfer and ancient paralogy of operons containing redundant copies of tryptophan-pathway genes in xylella species and in heterocystous cyanobacteria. | tryptophan-pathway genes that exist within an apparent operon-like organization were evaluated as examples of multi-genic genomic regions that contain phylogenetically incongruous genes and coexist with genes outside the operon that are congruous. a seven-gene cluster in xylella fastidiosa includes genes encoding the two subunits of anthranilate synthase, an aryl-coa synthetase, and trpr. a second gene block, present in the anabaena/nostoc lineage, but not in other cyanobacteria, contains a near ... | 2003 | 12620124 |
| transcriptome analysis of sinorhizobium meliloti during symbiosis. | rhizobia induce the formation on specific legumes of new organs, the root nodules, as a result of an elaborated developmental program involving the two partners. in order to contribute to a more global view of the genetics underlying this plant-microbe symbiosis, we have mined the recently determined sinorhizobium meliloti genome sequence for genes potentially relevant to symbiosis. we describe here the construction and use of dedicated nylon macroarrays to study simultaneously the expression of ... | 2003 | 12620125 |
| a real-time pcr assay for the detection of campylobacter jejuni in foods after enrichment culture. | a real-time pcr assay was developed for the quantitative detection of campylobacter jejuni in foods after enrichment culture. the specificity of the assay for c. jejuni was demonstrated with a diverse range of campylobacter species, related organisms, and unrelated genera. the assay had a linear range of quantification over six orders of magnitude, and the limit of detection was approximately 12 genome equivalents. the assay was used to detect c. jejuni in both naturally and artificially contami ... | 2003 | 12620820 |
| detection and typing of campylobacter jejuni and campylobacter coli and analysis of indicator organisms in three waterborne outbreaks in finland. | waterborne outbreaks associated with contamination of drinking water by campylobacter jejuni are rather common in the nordic countries sweden, norway, and finland, where in sparsely populated districts groundwater is commonly used without disinfection. campylobacters, escherichia coli, or other coliforms have rarely been detected in potential sources. we studied three waterborne outbreaks in finland caused by c. jejuni and used sample volumes of 4,000 to 20,000 ml for analysis of campylobacters ... | 2003 | 12620821 |
| comparison of broth microdilution, e test, and agar dilution methods for antibiotic susceptibility testing of campylobacter jejuni and campylobacter coli. | a standardized broth microdilution method was compared to the e test and an agar dilution method for the antimicrobial susceptibility testing of campylobacter jejuni and c. coli isolates. a group of 47 human clinical isolates, 37 isolates from retail poultry, and 29 isolates from living turkeys (total, 113 isolates) was included in the study. these encompassed 92 c. jejuni and 21 c. coli strains. the mics of six antimicrobial agents were determined by the broth microdilution and e test methods, ... | 2003 | 12624030 |
| molecular typing of campylobacter jejuni and campylobacter coli. | 2003 | 12624086 | |
| differential distribution of hla-dq beta/dr beta epitopes in the two forms of guillain-barré syndrome, acute motor axonal neuropathy and acute inflammatory demyelinating polyneuropathy (aidp): identification of dq beta epitopes associated with susceptibility to and protection from aidp. | guillain-barré syndrome (gbs), an acute, immune-mediated paralytic disorder affecting the peripheral nervous system, is the most common cause of acute flaccid paralysis in the post-polio era. gbs is classified into several subtypes based on clinical and pathologic criteria, with acute inflammatory demyelinating polyneuropathy (aidp) and acute motor axonal neuropathy (aman) being the most common forms observed. to better understand the pathogenesis of gbs and host susceptibility to developing the ... | 2003 | 12626563 |
| two families of mechanosensitive channel proteins. | mechanosensitive (ms) channels that provide protection against hypoosmotic shock are found in the membranes of organisms from the three domains of life: bacteria, archaea, and eucarya. two families of ubiquitous ms channels are recognized, and these have been designated the mscl and mscs families. a high-resolution x-ray crystallographic structure is available for a member of the mscl family, and extensive molecular genetic, biophysical, and biochemical studies conducted in many laboratories hav ... | 2003 | 12626684 |
| a revised annotation and comparative analysis of helicobacter pylori genomes. | huge amounts of genomic information are currently being generated. therefore, biologists require structured, exhaustive and comparative databases. the pylorigene database (http://genolist.pasteur.fr/pylorigene) was developed to respond to these needs, by integrating and connecting the information generated during the sequencing of two distinct strains of helicobacter pylori. this led to the need for a general annotation consensus, as the physical and functional annotations of the two strains dif ... | 2003 | 12626712 |
| rapid detection of escherichia coli o157:h7 inoculated in ground beef, chicken carcass, and lettuce samples with an immunomagnetic chemiluminescence fiber-optic biosensor. | a biosensor was evaluated with regard to its usefulness in the rapid detection of escherichia coli o157:h7 inoculated in ground beef, chicken carcass, and romaine lettuce samples. the biosensor consisted of a chemiluminescence reaction cell, a fiber-optic light guide, and a luminometer linked to a personal computer in conjunction with immunomagnetic separation. the samples inoculated with e. coli o157:h7 were first centrifuged and suspended in buffered peptone water and then incubated with anti- ... | 2003 | 12636312 |
| role of campylobacter jejuni/coli in diarrhoea in ile-ife, nigeria. | campylobacter jejuni/coli are well established causative agents of diarrhoea. in nigeria, gastroenteritis due to c. jejuni was first reported in northern part of the country in 1981 and the south-western part in ile-ife in 1983. | 2002 | 12638844 |
| drinking water contamination in walkerton, ontario: positive resolutions from a tragic event. | in may 2000, escherichia coli o157:h7 and campylobacter jejuni contaminated the drinking water supply in walkerton, ontario. seven people died and over 2,000 were ill as a result. the ontario provincial government set up a judicial inquiry into the circumstances surrounding the outbreak and also moved quickly to introduce a new drinking water regulation that incorporated some significant requirements for drinking water providers. the inquiry itself was in three parts: (a) part 1 related to the e ... | 2003 | 12638997 |
| elucidation of potential transmission routes of campylobacter in new zealand. | campylobacter is the most commonly reported notifiable disease in new zealand. the cost of campylobacter infections in the country during 1994 was estimated as dollar 61.7m although the true cost was probably higher. investigation of the main environmental reservoirs and routes of transmission to humans is necessary to formulate the most appropriate intervention strategies. this project investigated the reservoirs of campylobacter in a defined geographical area within new zealand and compared st ... | 2003 | 12639002 |
| abc of learning and teaching in medicine: written assessment. | 2003 | 12649242 | |
| occurrence of campylobacter spp. and cryptosporidium spp. in seagulls (larus spp.). | an investigation was carried out into the prevalence of thermophilic campylobacter subspecies (spp.) and cryptosporidium spp. in fresh fecal specimens collected from members of the gull family (larus spp.) from three coastal locations of northern ireland. a total of 205 fresh fecal specimens were collected from gulls, of which 28 of 205 (13.7%) were positive for campylobacter spp. and none of 205 for cryptosporidium spp. of these campylobacters, 21 of 28 (75%) isolates obtained belonged to the u ... | 2002 | 12653305 |
| quantitative, multiplexed detection of bacterial pathogens: dna and protein applications of the luminex labmap system. | escherichia coli, salmonella, listeria monocytogenes and campylobacter jejuni are bacterial pathogens commonly implicated in foodborne illnesses. generally used detection methods (i.e., culture, biochemical testing, elisa and nucleic acid amplification) can be laborious, time-consuming and require multiple tests to detect all of the pathogens. our objective was to develop rapid assays to simultaneously detect these four organisms through the presence of antigen or dna using the luminex labmap sy ... | 2003 | 12654495 |
| epidemiology of rifampin adp-ribosyltransferase (arr-2) and metallo-beta-lactamase (blaimp-4) gene cassettes in class 1 integrons in acinetobacter strains isolated from blood cultures in 1997 to 2000. | we characterized two new gene cassettes in an acinetobacter isolate: one harbored the metallo-beta-lactamase (imp-4) gene bla(imp-4), the other harbored the rifampin adp-ribosyltransferase (arr-2) gene arr-2, and both arrayed with the aminoglycoside acetyltransferase [aac(6')-ib(7)] gene cassette aaca4 in two separate class 1 integrons. the epidemiology of these gene cassettes in isolates from blood cultures obtained from 1997 to 2000 was studied. isolates bearing either the bla(imp-4) or the ar ... | 2003 | 12654674 |
| antibiotic susceptibility of campylobacter jejuni and campylobacter coli human isolates from bosnia and herzegovina. | 2003 | 12654755 | |
| flagella are virulence determinants of burkholderia pseudomallei. | burkholderia pseudomallei, a facultatively intracellular pathogen, is a flagellated and motile gram-negative bacterium and is the causative agent of melioidosis in humans. flagella are commonly recognized as important virulence determinants expressed by bacterial pathogens since the motility phenotype imparted by these organelles often correlates with the ability of an organism to cause disease. we used a virulent isolate of b. pseudomallei, khw, to construct an isogenic deletion mutant with a m ... | 2003 | 12654773 |
| helicobacter pylori mutants defective in ruvc holliday junction resolvase display reduced macrophage survival and spontaneous clearance from the murine gastric mucosa. | homologous recombination contributes to the extraordinary genetic diversity of helicobacter pylori and may be critical for surface antigen expression and adaptation to environmental challenges within the stomach. we generated isogenic, nonpolar h. pylori ruvc mutants to investigate the function of ruvc, a holliday junction endonuclease that resolves recombinant joints into nicked duplex products. inactivation of ruvc reduced the frequency of homologous recombination of h. pylori between 17- and ... | 2003 | 12654822 |
| campylobacter colonization in poultry: sources of infection and modes of transmission. | since its recognition as a human pathogen in the early 1970s, campylobacter jejuni has now emerged as the leading bacterial cause of food-borne gastroenteritis in developed countries. poultry, particularly chickens, account for the majority of human infections caused by campylobacter. reduction or elimination of this pathogen in the poultry reservoir is an essential step in minimizing the public health problem; however, farm-based intervention measures are still not available because of the lack ... | 2002 | 12665109 |
| [molecular typing by amplified fragment length polymorphism and pcr-restriction fragment length polymorphism , biotyping and antimicrobial susceptibility of campylobacter jejuni]. | molecular typing systems have provided invaluable information for tracking infectious agents through the food chain. these tools have been essential for understanding the epidemiology of gastrointestinal infectious diseases, therefore providing essential and evidence-based information for appropriate interventions and preventative measures. two such molecular typing techniques based on the polymerase chain reaction (pcr) that have been applied to the epidemiology of foodborne pathogens are, ampl ... | 2003 | 12666321 |
| bugging the bugs. genomics and proteomics may provide new ways of making the lives of bacteria more miserable. | 2003 | 12671675 | |
| production of diarrheal enterotoxins and other potential virulence factors by veterinary isolates of bacillus species associated with nongastrointestinal infections. | with the exceptions of bacillus cereus and bacillus anthracis, bacillus species are generally perceived to be inconsequential. however, the relevance of other bacillus species as food poisoning organisms and etiological agents in nongastrointestinal infections is being increasingly recognized. eleven bacillus species isolated from veterinary samples associated with severe nongastrointestinal infections were assessed for the presence and expression of diarrheagenic enterotoxins and other potentia ... | 2003 | 12676723 |
| prevalence of 11 pathogenic genes of campylobacter jejuni by pcr in strains isolated from humans, poultry meat and broiler and bovine faeces. | although many genes related to the pathogenicity of campylobacter jejuni have been reported, the relationships between these genes and the sources of strains are not clear. in this study, the presence of 11 pathogenic genes responsible for the expression of adherence, invasion, colonization and cytotoxin production was examined in 111 c. jejuni isolated from human clinical samples, poultry meat, broiler faeces and bovine faeces. for most of the pathogenic genes, no difference in their presence i ... | 2003 | 12676874 |
| antimicrobial resistance of campylobacter jejuni and campylobacter coli isolates from broiler chickens isolated at an irish poultry processing plant. | the antibiotic susceptibility of campylobacter jejuni and campylobacter coli isolates from broiler chickens were determined in order to evaluate the level of antibiotic resistance of campylobacter species in the irish poultry industry. | 2003 | 12680938 |
| prevalence of cytolethal distending toxin production in periodontopathogenic bacteria. | cytolethal distending toxin (cdt) is a newly identified virulence factor produced by several pathogenic bacteria implicated in chronic infection. seventy three strains of periodontopathogenic bacteria were examined for the production of cdt by a hela cell bioassay and for the presence of the cdt gene by pcr with degenerative oligonucleotide primers, which were designed based on various regions of the escherichia coli and campylobacter cdtb genes, which have been successfully used for the identif ... | 2003 | 12682119 |
| broadly reactive and highly sensitive assay for norwalk-like viruses based on real-time quantitative reverse transcription-pcr. | we have developed an assay for the detection of norwalk-like viruses (nlvs) based on reverse transcription-pcr (rt-pcr) that is highly sensitive to a broad range of nlvs. we isolated virus from 71 nlv-positive stool specimens from 37 outbreaks of nonbacterial acute gastroenteritis and sequenced the open reading frame 1 (orf1)-orf2 junction region, the most conserved region of the nlv genome. the data were subjected to multiple-sequence alignment analysis and similarity plot analysis. we used the ... | 2003 | 12682144 |
| characterization of encapsulated and noncapsulated haemophilus influenzae and determination of phylogenetic relationships by multilocus sequence typing. | a multilocus sequence typing (mlst) scheme has been developed for the unambiguous characterization of encapsulated and noncapsulated haemophilus influenzae isolates. the sequences of internal fragments of seven housekeeping genes were determined for 131 isolates, comprising a diverse set of 104 serotype a, b, c, d, e, and f isolates and 27 noncapsulated isolates. many of the encapsulated isolates had previously been characterized by multilocus enzyme electrophoresis (mlee), and the validity of t ... | 2003 | 12682154 |
| clonal dissemination of yersinia enterocolitica strains with various susceptibilities to nalidixic acid. | ten epidemiologically related yersinia enterocolitica clinical isolates were studied. six isolates were nalidixic acid resistant (mic > 512 microg/ml), with mutations in the quinolone resistance-determining region (qrdr) of the gyra gene, suggesting clonal dissemination of a nalidixic acid-susceptible y. enterocolitica strain which has acquired different mutations generating resistance to nalidixic acid. | 2003 | 12682183 |
| standardizing antimicrobial susceptibility testing of campylobacter species. | 2003 | 12682196 | |
| emerging water-borne pathogens. | emerging water-borne pathogens constitute a major health hazard in both developed and developing nations. a new dimension to the global epidemiology of cholera-an ancient scourge-was provided by the emergence of vibrio cholerae o139. also, water-borne enterohaemorrhagic escherichia coli ( e. coli o157:h7), although regarded as a problem of the industrialized west, has recently caused outbreaks in africa. outbreaks of chlorine-resistant cryptosporidium have motivated water authorities to reassess ... | 2003 | 12684849 |
| enhancement of disease and pathology by synergy of trichuris suis and campylobacter jejuni in the colon of immunologically naive swine. | campylobacterjejuni, a leading cause of bacterial gastroenteritis, has different age distribution and disease expression in developing and developed countries, which may be due to the endemnicity of infection and the age of acquisition of immunity. differences in disease expression are not solely dependent on the c. jejuni strain or virulence attributes. another modulating factor in developing countries may be endemic nematode infections such as trichuris, which drive type 2 cytokine responses a ... | 2003 | 12685626 |
| faecal contamination of greywater and associated microbial risks. | the faecal contamination of greywater in a local treatment system at vibyåsen, north of stockholm, sweden was quantified using faecal indicator bacteria and chemical biomarkers. bacterial indicator densities overestimated the faecal load by 100-1000-fold when compared to chemical biomarkers. based on measured levels of coprostanol, the faecal load was estimated to be 0.04 g person(-1) day(-1). prevalence of pathogens in the population and the faecal load were used to form the basis of a screenin ... | 2003 | 12688699 |
| campylobacter jejuni o:19 serotype-associated guillain-barré syndrome in a child: the first case reported from greece. | we present a case of guillain-barré syndrome (gbs) following campylobacter jejuni hs serotype o:19 infection in a child. antibodies against c. jejuni and autoantibodies to the peripheral nerve gangliosides gm1 were positive, a pattern correlating well with the existence of an inflammatory neuropathy like gbs. the patient shared the hla-b35 and hla-dr8 antigens, which have been found to be increased in gbs patients with previous c. jejuni infection. as this is the first diagnosed c. jejuni-associ ... | 2003 | 12691547 |
| acanthamoeba spp. as agents of disease in humans. | acanthamoeba spp. are free-living amebae that inhabit a variety of air, soil, and water environments. however, these amebae can also act as opportunistic as well as nonopportunistic pathogens. they are the causative agents of granulomatous amebic encephalitis and amebic keratitis and have been associated with cutaneous lesions and sinusitis. immuno compromised individuals, including aids patients, are particularly susceptible to infections with acanthamoeba. the immune defense mechanisms that op ... | 2003 | 12692099 |
| survival of campylobacter jejuni strains of different origin in drinking water. | the aim of the study was to measure the survival of 19 campylobacter jejuni strains of different origins, including two reference strains, four poultry-derived isolates, nine human isolates and four water isolates, in sterilized drinking water. | 2003 | 12694454 |
| structure and genotypic plasticity of the campylobacter fetus sap locus. | the campylobacter fetus surface layer proteins (slps), encoded by five to nine sapa homologues, are major virulence factors. to characterize the sapa homologues further, a 65.9 kb c. fetus genomic region encompassing the sap locus from wild-type strain 23d was completely sequenced and analysed; 44 predicted open reading frames (orfs) were recognized. the 53.8 kb sap locus contained eight complete and one partial sapa homologues, varying from 2769 to 3879 bp, sharing conserved 553-2622 bp 5' regi ... | 2003 | 12694614 |
| comparing bacterial genomes through conservation profiles. | we constructed two-dimensional representations of profiles of gene conservation across different genomes using the genome of escherichia coli as a model. these profiles permit both the visualization at the genome level of different traits in the organism studied and, at the same time, reveal features related to the genomes analyzed (such as defective genomes or genomes that lack a particular system). conserved genes are not uniformly distributed along the e. coli genome but tend to cluster toget ... | 2003 | 12695324 |
| reduction of campylobacter jejuni on poultry by low-temperature treatment. | campylobacter jejuni is a leading cause of acute bacterial gastroenteritis in the united states, with epidemiologic studies identifying poultry as a leading vehicle in human infection. studies were conducted to determine rates of c. jejuni inactivation on poultry exposed to different cooling and freezing temperatures. a mixture of three strains of c. jejuni originally isolated from poultry was inoculated onto chicken wings at ca. 10(7) cfu/g. the results of the study revealed that the storage of ... | 2003 | 12696690 |
| rapid detection of biofilms and adherent pathogens using scanning confocal laser microscopy and episcopic differential interference contrast microscopy. | knowledge of biofilm structure and function has changed significantly in the last few years due to advances in light microscopy. one pertinent example is the use of scanning confocal laser microscopy (sclm) to visualise corrosion pits caused by the biofilm mosaic footprint on corroding metal surfaces. nevertheless, sclm has some limitations as to its widespread use, including cost, inability to observe motile bacteria and eukaryotic grazers within biofilms, and difficulty to scan a curved surfac ... | 2003 | 12701914 |
| microbial genome jambalaya. | 2003 | 12702204 | |
| induction of proinflammatory responses in the human monocytic cell line thp-1 by campylobacter jejuni. | campylobacter jejuni can cause an enteritis that is associated with an acute inflammatory response at the gut epithelial surface. the signals inducing inflammation are unknown. c. jejuni can penetrate the intestinal epithelial barrier and may then interact with leucocytes, potentially inducing proinflammatory responses. to investigate this, we studied the interaction of c. jejuni with the human monocytic cell line thp-1 and show that a range of proinflammatory cytokines and chemokines is induced ... | 2003 | 12704137 |
| igg antibodies against microorganisms and atopic disease in danish adults: the copenhagen allergy study. | seropositivity to food-borne and orofecal microorganisms (hepatitis a virus, helicobacter pylori, and toxoplasma gondii ), which are considered to be markers of poor hygiene, has been reported to be associated with a lower prevalence of atopy. in contrast, colonization of the gut with clostridium difficile, a potential intestinal bacterial pathogen, in early childhood may be associated with a higher prevalence of atopy. | 2003 | 12704368 |
| immunological characterization of the campylobacter jejuni 72dz/92 cjad gene product and its fusion with b subunit of e. coli lt toxin. | campylobacter jejuni 72dz/92 cjad gene, orthologue of c. jejuni nctc 11168 cj0113, c. jejuni m275 omp18 and c. jejuni atcc 29428 omp18, has been cloned, sequenced and analysed from the viewpoint of its immunological attributes. neither the 5' nor 3' fragment of the cjad encodes protein capable of reacting with anti-campylobacter antibodies. several fusions of the cjad with eltb, which encodes b subunit of the e. coli lt toxin, have been constructed. the hybrid proteins, which differ in respect t ... | 2002 | 12708820 |
| domestic poultry-raising practices in a peruvian shantytown: implications for control of campylobacter jejuni-associated diarrhea. | raising poultry at home is common in many periurban communities in low-income countries. studies demonstrate that free-range domestic poultry increase children's risk of infection with diarrhea-causing organisms such as campylobacter jejuni. corralling might reduce risk, but research on the socioeconomic acceptability of corralling is lacking. to explore this issue, we studied local knowledge and practices related to poultry-raising in a peruvian shantytown. our objectives were to understand: (1 ... | 2003 | 12711102 |
| detection of campylobacter jejuni strains in the water lines of a commercial broiler house and their relationship to the strains that colonized the chickens. | campylobacter jejuni is frequently present in the intestinal tract of commercial broiler chickens, and their drinking water has been proposed to be an initial source of bacteria for newly hatched chicks. we studied three sequential commercial broiler flocks raised in a house from which we had cultured c. jejuni from the nipple waters prior to placement of the first flock. campylobacter cells were detected by immunofluorescence in the biofilm of the drinking nipples during the weeks when the floc ... | 2003 | 12713164 |
| investigation of infectious agents associated with arthritis by reverse transcription pcr of bacterial rrna. | in reactive and postinfectious arthritis the joints are generally sterile but the presence of bacterial antigens and nucleic acids has been reported. to investigate whether organisms traffic to affected joints in these conditions, we performed reverse transcription pcr using universal primers to amplify any bacterial 16s rrna sequences present in synovial fluid. bacterial sequences were detected in most cases, even after treatment of the synovial fluid with dnase, implying the presence of bacter ... | 2003 | 12716447 |
| detection of conserved n-linked glycans and phase-variable lipooligosaccharides and capsules from campylobacter cells by mass spectrometry and high resolution magic angle spinning nmr spectroscopy. | glycomics, the study of microbial polysaccharides and genes responsible for their formation, requires the continuous development of rapid and sensitive methods for the identification of glycan structures. in this study, methods for the direct analysis of sugars from 108 to 1010 cells are outlined using the human gastrointestinal pathogen, campylobacter jejuni. using capillary-electrophoresis coupled with sensitive electrospray mass spectrometry, we demonstrate variability in the lipid a componen ... | 2003 | 12716884 |
| diarrhea incidence and farm-related risk factors for escherichia coli o157:h7 and campylobacter jejuni antibodies among rural children. | serum samples were obtained from 215 farm-resident children and 396 non-farm-resident children living in a defined rural wisconsin population. antibodies to campylobacter jejuni and escherichia coli o157:h7 lipopolysaccharide (o157 lps) immunoglobulin g were measured, and the incidence of clinic visits for diarrheal illness was determined. risk factors were assessed in a telephone interview. there were 363 children (59%) with c. jejuni antibodies (seropositive for >or=2 immunoglobulin classes) a ... | 2003 | 12717628 |
| [campylobacter (jejuni/coli)]. | 2003 | 12718055 | |
| inhibition of helicobacter pylori growth in vitro by bulgarian propolis: preliminary report. | bee glue (propolis) possesses antimicrobial, anti-inflammatory, anaesthetic and immunostimulating activities. the aim of the study was to evaluate the inhibitory effect of bulgarian propolis on helicobacter pylori growth in vitro. activity of 30% ethanolic extract of propolis (eep) against 38 clinical isolates of h. pylori was evaluated by using the agar-well diffusion method. ethanol was used as a control. in addition, the effect of propolis on the growth of 26 h. pylori and 18 campylobacter st ... | 2003 | 12721318 |
| a one-year study of campylobacter carriage by individual danish broiler chickens as the basis for selection of campylobacter spp. strains for a chicken infection model. | from february 1999 to february 2000, 1,250 individual broiler chickens representing 125 broiler flocks originating from 62 broiler farms in denmark were screened for campylobacter carriage. every month, 10 flocks were tested for campylobacter carriage. the swabs were tested individually and as a pooled sample representing the flocks. campylobacter spp. carriage was detected from 512 (40.9%) broiler chickens originating from 63 (50.4%) positive flocks. campylobacter carriage by both individual ch ... | 2003 | 12729201 |
| health problems following campylobacter jejuni enteritis in a lancashire population. | campylobacter jejuni enteritis can lead to musculoskeletal, neuropathic or other health sequelae. we investigated the coexistence, seasonal occurrence, strain-type associations and impact on work capacity of different health problems following c. jejuni enteritis in a lancashire population during 1999 and 2001. | 2003 | 12730525 |
| enteric pathogens and soil: a short review. | it is known that soil is a recipient of solid wastes able to contain enteric pathogens in high concentrations. although the role of soil as a reservoir of certain bacterial pathogens is not in question, recent findings show that soil may have a larger role in the transmission of enteric diseases than previously thought. many of the diseases caused by agents from soil have been well characterized, although enteric diseases and their link to soil have not been so well studied. gastrointestinal inf ... | 2003 | 12730707 |
| wavelet to predict bacterial ori and ter: a tendency towards a physical balance. | chromosomal dna replication in bacteria starts at the origin (ori) and the two replicores propagate in opposite directions up to the terminus (ter) region. we hypothesize that the two replicores need to reach ter at the same time to maintain a physical balance; dna insertion would disrupt such a balance, requiring chromosomal rearrangements to restore the balance. to test this hypothesis, we needed to demonstrate that ori and ter are in a physical balance in bacterial chromosomes. using wavelet ... | 2003 | 12732098 |
| in vivo tracking of campylobacter jejuni by using a novel recombinant expressing green fluorescent protein. | campylobacter jejuni is a leading cause of food-borne disease in developed countries. the goal of this study was to develop a plasmid-based reporter system with green fluorescent protein (gfp) to facilitate the study of c. jejuni in a variety of niches. c. jejuni transformants harboring the pmek91 gfp gene (gfp)-containing vector were readily detectable by both fluorescence microscopy and flow cytometry. given the ease of detecting these organisms, additional experiments were performed in which ... | 2003 | 12732559 |
| antimicrobial-resistant campylobacter species from retail raw meats. | the antimicrobial susceptibilities of 378 campylobacter isolates were determined. resistance to tetracycline was the most common (82%), followed by resistance to doxycycline (77%), erythromycin (54%), nalidixic acid (41%), and ciprofloxacin (35%). campylobacter coli displayed significantly higher rates of resistance to ciprofloxacin and erythromycin than campylobacter jejuni, and campylobacter isolates from turkey meat showed a greater resistance than those from chicken meat. | 2003 | 12732579 |
| evaluation of a new cellulose sponge-tipped swab for microbiological sampling: a laboratory and clinical investigation. | a new type of swab (cellswab; cellomeda, turku, finland), utilizing a highly absorbent cellulose viscose sponge material, was compared to some traditional swabs. the survival of 14 aerobic and 10 anaerobic and microaerophilic bacterial species in the cellswab, two commercial swab transport systems (copan, brescia, italy, and orion diagnostica, espoo, finland), and one dacron swab (technical service consultants ltd. [tsc], heywood, united kingdom) was evaluated. bacteria were suspended in broth, ... | 2003 | 12734223 |
| usefulness of the microseq 500 16s ribosomal dna-based bacterial identification system for identification of clinically significant bacterial isolates with ambiguous biochemical profiles. | due to the inadequate automation in the amplification and sequencing procedures, the use of 16s rrna gene sequence-based methods in clinical microbiology laboratories is largely limited to identification of strains that are difficult to identify by phenotypic methods. in this study, using conventional full-sequence 16s rrna gene sequencing as the "gold standard," we evaluated the usefulness of the microseq 500 16s ribosomal dna (rdna)-based bacterial identification system, which involves amplifi ... | 2003 | 12734240 |
| anti-gm1b igg antibody is associated with acute motor axonal neuropathy and campylobacter jejuni infection. | anti-gm1 and anti-gm1b antibodies are frequently present in patients with guillain-barré syndrome (gbs) and accordingly, the two antibodies often coexist in the same patient. in order to study clinical and laboratory features of anti-gm1b-positive gbs, we analyzed the data of patients with anti-gm1b igg antibody but no anti-gm1 igg antibody. of 86 consecutive patients, 10 had anti-gm1b antibody alone and frequently had acute motor axonal neuropathy (aman, 80%) and campylobacter jejuni infection ... | 2003 | 12736086 |
| longitudinal changes of anti-ganglioside antibodies before and after guillain-barré syndrome onset subsequent to campylobacter jejuni enteritis. | anti-ganglioside antibodies frequently are present in sera from patients with guillain-barré syndrome (gbs) during the acute phase, but no patients in whom anti-ganglioside antibodies were tested before the onset of the syndrome have been reported. we describe the first case of gbs subsequent to campylobacter jejuni infection, in which longitudinal changes in anti-ganglioside antibody titers were measured before and after the onset of limb weakness. serum antibody titers against gm1 (igm/igg), g ... | 2003 | 12736097 |
| immunoglobulin km genes in guillain-barré syndrome. | guillain-barré syndrome is associated with antecedent campylobacter jejuni infection. only a minority of the infected individuals, however, develops the disease, implying a role for genetic factors in conferring susceptibility. to determine the role of immunoglobulin km genes (genetic markers of the constant region of kappa chains) in the etiology of this syndrome, we genotyped 83 patients and 196 healthy controls from norway for km1 and km3 alleles by polymerase chain reaction-restriction fragm ... | 2003 | 12736802 |
| a comparison of campylobacter jejuni enteritis incidence rates in high- and low-poultry-density counties: michigan 1992-1999. | to compare the incidence of campylobacter jejuni enteritis in high- and low-poultry-density counties in michigan between the years 1992 and 1999, an ecological study was conducted in the state of michigan. a log-linear model was used to compare yearly, seasonal, age, and gender-specific incidence rates between county groupings. counties with a high poultry density had a higher overall incidence of c. jejuni enteritis, particularly among children and young adults, compared with counties with low ... | 2002 | 12737543 |
| one-step immunochromatographic dipstick tests for rapid detection of vibrio cholerae o1 and o139 in stool samples. | we describe the development and evaluation of a rapid diagnostic test for vibrio cholerae o1 and o139 based on lipopolysaccharide detection using gold particles. the specificity ranged between 84 and 100%. the sensitivity of the dipsticks ranged from 94.2 to 100% when evaluated with stool samples obtained in madagascar and bangladesh. the dipstick can provide a simple tool for epidemiological surveys. | 2003 | 12738652 |
| anti-gm1 antibody igg subclass: a clinical recovery predictor in guillain-barré syndrome. | to determine whether the anti-gm1 antibody igg subclass (igg1 to 4) is associated with clinical profiles and patterns of recovery in guillain-barré syndrome (gbs). | 2003 | 12743241 |
| a theoretical approach to assess microbial risks due to failures in drinking water systems. | a failure in treatment or in the distribution network of a surface water-works could have serious consequences due to the variable raw water quality in combination with an extended distribution. the aim of this study was to examine the theoretical impact of incidents in the drinking water system on the annual risk of infection in a population served by a large water treatment plant in sweden. reported incidents in the system were examined and a microbial risk assessment that included three patho ... | 2003 | 12745338 |
| phenotypic and genotypic differentiation of campylobacter spp. isolated from austrian broiler farms: a comparison. | an identification scheme based on restriction fragment length polymorphism of polymerase chain reaction products (pcr-rflp) was developed to differentiate isolates of the genera campylobacter, arcobacter and helicobacter. based on the 16s rrna gene of these genera, pcr amplified a 1216-bp fragment. the amplicons were digested with the restriction enzymes rsai and ecorv. additional differentiation was obtained using a pcr-assay based on the hippuricase gene. genotyping was performed on several re ... | 2003 | 12745378 |
| pcr detection of seven virulence and toxin genes of campylobacter jejuni and campylobacter coli isolates from danish pigs and cattle and cytolethal distending toxin production of the isolates. | to study the prevalence of seven virulence and toxin genes, and cytolethal distending toxin (cdt) production of campylobacter jejuni and c. coli isolates from danish pigs and cattle. | 2003 | 12752808 |
| functional subsets of the virb type iv transport complex proteins involved in the capacity of agrobacterium tumefaciens to serve as a recipient in virb-mediated conjugal transfer of plasmid rsf1010. | the virb-encoded type iv transport complex of agrobacterium tumefaciens mediates the transfer of dna and proteins into plant cells, as well as the conjugal transfer of incq plasmids, such as rsf1010, between agrobacterium strains. while several studies have indicated that there are physical interactions among the 11 virb proteins, the functional significance of the interactions has been difficult to establish since all of the proteins are required for substrate transfer. our previous studies, ho ... | 2003 | 12754223 |
| phosphorylation of the lipid a region of meningococcal lipopolysaccharide: identification of a family of transferases that add phosphoethanolamine to lipopolysaccharide. | a gene, nmb1638, with homology to the recently characterized gene encoding a phosphoethanolamine transferase, lpt-3, has been identified from the neisseria meningitidis genome sequence and was found to be present in all meningococcal strains examined. homology comparison with other database sequences would suggest that nmb1638 and lpt-3 represent genes coding for members of a family of proteins of related function identified in a wide range of gram-negative species of bacteria. when grown and is ... | 2003 | 12754224 |
| recognition of the 5' leader of pre-trna substrates by the active site of ribonuclease p. | the bacterial trna processing enzyme ribonuclease p (rnase p) is a ribonucleoprotein composed of a approximately 400 nucleotide rna and a smaller protein subunit. it has been established that rnase p rna contacts the mature trna portion of pre-trna substrates, whereas rnase p protein interacts with the 5' leader sequence. however, specific interactions with substrate nucleotides flanking the cleavage site have not previously been defined. here we provide evidence for an interaction between a con ... | 2003 | 12756331 |
| identification of beta-subunit of bacterial rna-polymerase--a non-species-specific bacterial protein--as target of antibodies in primary biliary cirrhosis. | several observations suggest that bacteria induce autoimmunity in primary biliary cirrhosis (pbc). since no pbc-specific bacterial species could be identified, it can be speculated that the triggers are non-species-specific bacterial proteins. this hypothesis would imply that several or even all bacterial species can trigger pbc. therefore, we investigated whether pbc exhibits immune reactions to non-species-specific bacterial antigens. yersinia enterocolitica o3 was screened for the presence of ... | 2003 | 12757171 |
| antimicrobial resistance of campylobacter jejuni subsp. jejuni strains isolated from humans in 1998 to 2001 in montréal, canada. | the rates of resistance of 51 to 72 human strains of campylobacter jejuni subsp. jejuni isolated annually from 1998 to 2001 in montréal, québec, canada, varied from 1 to 12% for erythromycin, 43 to 68% for tetracycline, and 10 to 47% for ciprofloxacin. in the last years of the study, there was a significant increase in the rate of resistance to ciprofloxacin (p = 0.00003) but not in the rate of resistance to erythromycin (p = 0.056) or tetracycline (p = 0.095) compared to the rate obtained in th ... | 2003 | 12760892 |
| campylobacter jejuni enteritis associated with consumption of raw milk. | an outbreak of campylobacter jejuni enteritis occurred among people who had attended a meal where raw milk was served. a case control study was conducted using instances of illness as cases; those who attended the event but did not become ill served as controls. thirteen of 20 people who had attended the meal became ill. c. jejuni was cultured from five of six stools that were submitted. raw milk consumption was strongly associated with illness (p = .0072, fisher exact test). although c. jejuni ... | 2003 | 12762121 |
| new born chicks can serve as an experimental animal model for human campylobacteriosis. | campylobacter enteritis is an emerging food borne zoonotic disease. improperly cooked chicken serve as a potential source for this infection. diarrheogenic potential of campylobacter jejuni is tested either by in-vivo rat ileal loop (ril) test or by molecular methods. this study reveals that 3-day-old chicks can serve as an animal model for toxigenic c. jejuni. | 2002 | 12776802 |
| cloning, preliminary characterization and crystallization of nucleoside hydrolases from caenorhabditis elegans and campylobacter jejuni. | the nucleoside hydrolases (nhs) are a family of nucleoside-modifying enzymes. they play an important role in the purine-salvage pathway of many pathogenic organisms which are unable to synthesize purines de novo. although well characterized in protozoan parasites, their precise function and mechanism remain unclear in other species. for the first time, nhs from caenorhabditis elegans and campylobacter jejuni, which are representatives of mesozoa and bacteria, respectively, have been cloned and p ... | 2003 | 12777783 |
| seasonal variation in bacterial pathogens isolated from stool samples in karachi, pakistan. | to determine the seasonal variation of the commonly isolated bacterial pathogens in stool samples. | 2003 | 12779031 |
| campylobacter--a tale of two protein glycosylation systems. | post-translational glycosylation is a universal modification of proteins in eukarya, archaea and bacteria. two recent publications describe the first confirmed report of a bacterial n-linked glycosylation pathway in the human gastrointestinal pathogen campylobacter jejuni. in addition, an o-linked glycosylation pathway has been identified and characterized in c. jejuni and the related species campylobacter coli. both pathways have similarity to the respective n- and o-linked glycosylation proces ... | 2003 | 12781527 |
| campylobacter jejuni contamination on broiler carcasses of c. jejuni-negative flocks during processing in a japanese slaughterhouse. | at a slaughterhouse, we determined the campylobacter jejuni status of each broiler flock coming from different farms by culturing cecal contents of representative birds, and examined the prevalence of c. jejuni on carcasses during processing. then, c. jejuni isolates from the carcasses were typed with rapd pcr and compared with those from the cecal contents of a c. jejuni-positive flock. c. jejuni was not isolated from the carcasses of c. jejuni-negative flocks processed before the c. jejuni-pos ... | 2003 | 12781960 |
| characterization of urease-positive thermophilic campylobacter subspecies by multilocus enzyme electrophoresis typing. | thirty-one urease-positive thermophilic campylobacter (uptc) isolates, including three reference strains (nctc12892, nctc12895 and nctc12896), and three campylobacter lari isolates, which were isolated from several countries and sources, were compared genotypically by using multilocus enzyme electrophoresis (mlee). we examined allelic variation around seven enzyme loci, including the adenylate kinase, alkaline phosphatase, catalase, fumarase, malic enzyme, malate dehydrogenase, and l-phenylalany ... | 2003 | 12788730 |
| use of pcr for direct detection of campylobacter species in bovine feces. | this study reports on the use of pcr to directly detect and distinguish campylobacter species in bovine feces without enrichment. inhibitors present in feces are a major obstacle to using pcr to detect microorganisms. the qiaamp dna stool minikit was found to be an efficacious extraction method, as determined by the positive amplification of internal control dna added to bovine feces before extraction. with nested or seminested multiplex pcr, campylobacter coli, c. fetus, c. hyointestinalis, and ... | 2003 | 12788747 |
| rapid detection of campylobacter coli, c. jejuni, and salmonella enterica on poultry carcasses by using pcr-enzyme-linked immunosorbent assay. | contamination of retail poultry by campylobacter spp. and salmonella enterica is a significant source of human diarrheal disease. isolation and identification of these microorganisms require a series of biochemical and serological tests. in this study, campylobacter ceue and salmonella inva genes were used to design probes in pcr-enzyme-linked immunosorbent assay (elisa), as an alternative to conventional bacteriological methodology, for the rapid detection of campylobacter jejuni, campylobacter ... | 2003 | 12788755 |
| rapid screening of chicken intestinal contents for campylobacter jejuni using coagglutination. | 2003 | 12790236 | |
| multilocus sequence typing system for group b streptococcus. | a multilocus sequence typing (mlst) system was developed for group b streptococcus (gbs). the system was used to characterize a collection (n = 152) of globally and ecologically diverse human strains of gbs that included representatives of capsular serotypes ia, ib, ii, iii, v, vi, and viii. fragments (459 to 519 bp) of seven housekeeping genes were amplified by pcr for each strain and sequenced. the combination of alleles at the seven loci provided an allelic profile or sequence type (st) for e ... | 2003 | 12791877 |
| species-specific identification of campylobacters by partial 16s rrna gene sequencing. | species-specific identification of campylobacters is problematic, primarily due to the absence of suitable biochemical assays and the existence of atypical strains. 16s rrna gene (16s rdna)-based identification of bacteria offers a possible alternative when phenotypic tests fail. therefore, we evaluated the reliability of 16s rdna sequencing for the species-specific identification of campylobacters. sequence analyses were performed by using almost 94% of the complete 16s rrna genes of 135 phenot ... | 2003 | 12791878 |
| molecular characterization of isolates of waterborne cryptosporidium spp. collected during an outbreak of gastroenteritis in south burgundy, france. | in september 2001, a waterborne outbreak of gastroenteritis occurred in eastern france. of 31 fecal samples from symptomatic individuals, 19 tested positive for cryptosporidium with two pcrs targeting the hsp70 and the 18s rrna genes of cryptosporidium: sequencing of the pcr fragments produced sequences identical to that of cryptosporidium parvum genotype 1. | 2003 | 12791906 |
| a descriptive study of guillain-barré syndrome in high and low campylobacter jejuni incidence regions of michigan: 1992-1999. | the incidence rate of guillain-barré syndrome (gbs) was compared between high and low campylobacter jejuni incidence counties in michigan, usa, between 1992 and 1999. data on gbs was obtained from cases reported to the michigan department of community health. poisson confidence intervals were used to compare incidence rates. there was no significant difference in the overall rate between high and low c. jejuni incidence regions in michigan. though no differences in age-specific, gender-specific, ... | 2003 | 12792145 |
| induction of an adaptive tolerance response in the foodborne pathogen, campylobacter jejuni. | in this study we aimed to determine if campylobacter had the ability to induce an adaptive tolerance response (atr) to acid and/or aerobic conditions. campylobacter jejuni ci 120 was grown to the appropriate phase in brucella broth under microaerobic conditions. cells were initially adapted to a mild stress (ph 5.5) for 5 h prior to challenge at ph 4.5, a lethal ph. survival was examined by determining the numbers of viable cells on campylobacter blood free selective agar base. stationary phase ... | 2003 | 12799005 |
| effects on axonal conduction of anti-ganglioside sera and sera from patients with guillain-barré syndrome. | the efficacy of plasma exchange as a therapy for guillain-barré syndrome (gbs) suggests that humoral factors might contribute to the axonal conduction block responsible for the major symptoms of the disease. to explore this possibility, we have applied sera to rat spinal roots in vitro while monitoring axonal conduction. neither fresh sera from 12 patients with gbs or miller-fisher syndrome (mfs), nor serum from rabbits immunised with campylobacter jejuni from patients with gbs, mfs or gastroent ... | 2003 | 12799030 |
| comparison of eight phenotypic methods for subspecies characterization of thermophilic campylobacter spp. isolated from pig liver. | four hundred pork livers from bacon pigs (37 herds) obtained at six pig-processing plants were studied to assess the campylobacter contamination rate. deep tissue areas were sampled immediately after evisceration. approximately 6% of livers were infected with campylobacter spp., including campylobacter coli (67%), campylobacter jejuni (30%), and campylobacter lari (3%). the 60 resulting isolates (39 c. coli isolates, 19 c. jejuni isolates, and 2 c. lari isolates) employed in this study were char ... | 2003 | 12801013 |
| a bacterial toxin that causes dna damage to modulate cellular responses. | campylobacter jejuni constitutes the leading cause of bacterial diarrhea in the u.s. and all around the world. this common bacterium produces a toxin known as cytolethal distending toxin (cdt) which causes intoxicated cells to enlarge and to stop dividing with a double dna content characteristic of g2/m arrest. the effect of the toxin on the cell is so striking that it captivated scientists for a long time. however, its mechanism of action had remained elusive. | 2001 | 12805665 |
| antibiotic resistance in campylobacter jejuni isolated from humans in the hunter region, new south wales. | campylobacter is a common cause of bacterial gastroenteritis in australia. antibiotic resistance among campylobacter is an emerging problem in europe and the united states of america. monitoring may detect emerging resistance. since there is no epidemiologically validated subtyping system for campylobacter, antimicrobial resistance patterns may prove useful as an epidemiological marker. campylobacter isolates from residents of the hunter region were differentiated by pcr into two categories: c. ... | 2003 | 12807280 |
| the complete genome sequence of the carcinogenic bacterium helicobacter hepaticus. | helicobacter hepaticus causes chronic hepatitis and liver cancer in mice. it is the prototype enterohepatic helicobacter species and a close relative of helicobacter pylori, also a recognized carcinogen. here we report the complete genome sequence of h. hepaticus atcc51449. h. hepaticus has a circular chromosome of 1,799,146 base pairs, predicted to encode 1,875 proteins. a total of 938, 953, and 821 proteins have orthologs in h. pylori, campylobacter jejuni, and both pathogens, respectively. h. ... | 2003 | 12810954 |