Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| a universal method for species identification of mammals utilizing next generation sequencing for the analysis of dna mixtures. | species identification can be interesting in a wide range of areas, for example, in forensic applications, food monitoring and in archeology. the vast majority of existing dna typing methods developed for species determination, mainly focuses on a single species source. there are, however, many instances where all species from mixed sources need to be determined, even when the species in minority constitutes less than 1 % of the sample. the introduction of next generation sequencing opens new po ... | 2013 | 24358309 |
| gastric endoscopic submucosal dissection: from animal model to patient. | to assess whether the use of porcine models is useful for learning endoscopic submucosal dissection (esd), thus contributing to its subsequent application in human patients. | 0 | 24363524 |
| prevalence of african swine fever virus in apparently healthy domestic pigs in uganda. | african swine fever (asf) is a contagious viral disease which can cause up to 100% mortality among domestic pigs leading to serious socio-economic impact on people's livelihoods. asf is endemic in uganda and there is paucity of information on the epidemiology of the disease. the major aim of this study was to determine the seroprevalence and prevalence of african swine fever virus (asfv) in apparently healthy slaughter pigs at wambizi slaughterhouse in kampala city, uganda. we also estimated the ... | 2013 | 24369729 |
| qualitative tissue differentiation by analysing the intensity ratios of atomic emission lines using laser induced breakdown spectroscopy (libs): prospects for a feedback mechanism for surgical laser systems. | the research work presented in this paper focuses on qualitative tissue differentiation by monitoring the intensity ratios of atomic emissions using 'laser induced breakdown spectroscopy' (libs) on the plasma plume created during laser tissue ablation. the background of this study is to establish a real time feedback control mechanism for clinical laser surgery systems during the laser ablation process. ex-vivo domestic pig tissue samples (muscle, fat, nerve and skin) were used in this experimen ... | 2015 | 24376030 |
| retrograde flush is more protective than heparin in the uncontrolled donation after circulatory death lung donor. | formation of microthrombi after circulatory arrest is a concern for the development of reperfusion injury in lung recipients from donation after circulatory death (dcd) donors. in this isolated lung reperfusion study, we compared the effect of postmortem heparinization with preharvest retrograde pulmonary flush or both. | 2014 | 24378013 |
| a novel sp1/sp3 dependent intronic enhancer governing transcription of the ucp3 gene in brown adipocytes. | uncoupling protein (ucp) 3 is a mitochondrial inner membrane protein implicated in lipid handling and metabolism of reactive oxygen species. its transcription is mainly regulated by peroxisome proliferator-activated receptors (ppar), a family of nuclear hormone receptors. employing bandshift assays, rna interference and reporter gene assays we examine an intronic region in the ucp3 gene harboring a cis-element essential for expression in brown adipocytes. we demonstrate binding of sp1 and sp3 to ... | 2013 | 24391766 |
| seroprevalence of porcine proliferative enteropathy among wild boars in the republic of korea. | the importance of the wild boar as a reservoir of lawsonia intracellularis was assessed by investigating the seroprevalence of this pathogen among wild boars in the republic of korea. the extent of exposure to l. intracellularis among wild boars (sus scrofa coreanus) was monitored by a country-wide serological survey using an immunoperoxidase monolayer assay. | 2014 | 24393381 |
| shiga toxin-producing escherichia coli in swine: the public health perspective. | shiga toxin-producing escherichia coli (stec) strains are food-borne pathogens that are an important public health concern. stec infection is associated with severe clinical diseases in human beings, including hemorrhagic colitis (hc) and hemolytic uremic syndrome (hus), which can lead to kidney failure and death. cattle are the most important stec reservoir. however, a number of stec outbreaks and hus cases have been attributed to pork products. in swine, stec strains are known to be associated ... | 2014 | 24397985 |
| corrosion casting of the subglottis following endotracheal tube intubation injury: a pilot study in yorkshire piglets. | subglottic stenosis can result from endotracheal tube injury. the mechanism by which this occurs, however, is not well understood. the purpose of this study was to examine the role of angiogenesis, hypoxia and ischemia in subglottic mucosal injury following endotracheal intubation. | 2013 | 24401165 |
| the limit of anemia tolerance during hyperoxic ventilation with pure oxygen in anesthetized domestic pigs. | during acellular replacement of an acute blood loss, hyperoxic ventilation (hv) increases the amount of o2 physically dissolved in the plasma and thereby improves o2 supply to the tissues. while this effect could be demonstrated for hv with inspiratory o2 fraction (fio2) 0.6, it was unclear whether hv with pure oxygen (fio2 1.0) would have an additional effect on the physiological limit of acute normovolemic anemia. | 2013 | 24401552 |
| spatial multi-criteria decision analysis to predict suitability for african swine fever endemicity in africa. | african swine fever (asf) is endemic in several countries of africa and may pose a risk to all pig producing areas on the continent. official asf reporting is often rare and there remains limited awareness of the continent-wide distribution of the disease.in the absence of accurate asf outbreak data and few quantitative studies on the epidemiology of the disease in africa, we used spatial multi-criteria decision analysis (mcda) to derive predictions of the continental distribution of suitability ... | 2014 | 24406022 |
| development of an animal model of fibrous cholangitis in pigs. | the aim of this study was to develop a model of fibrous cholangitis in the offspring of gravid domestic pigs through the administration of 1,4-phenylene diisothiocyanate (ditc). | 2014 | 24410545 |
| efficacy of marker vaccine candidate cp7_e2alf against challenge with classical swine fever virus isolates of different genotypes. | classical swine fever (csf) is among the most important viral disease of domestic and feral pigs and has a serious impact on animal health and pig industry. in most countries with industrialized pig production, prophylactic vaccination against csf is banned, and all efforts are directed towards eradication of the disease, e.g. by culling of infected herds and animal movement restrictions. nevertheless, emergency vaccination remains an option to minimize the socio-economic impact of outbreaks. fo ... | 2014 | 24411658 |
| cell proliferation in the seminiferous and epididymal epithelia of sus domesticus. | it is important to understand the proliferative activity of the different structures of the male reproductive apparatus in livestock species, such as sus domesticus, to ensure reproductive efficiency. the main aims of this study were (a) to evaluate the proliferative activity of the spermatogonia in the different stages of the seminiferous cycle and (b) to study the cell proliferation in the epididymal epithelium in each region, identifying the different cells involved. for this, the testes and ... | 2014 | 24418529 |
| chromosome translocations in the karyotypes of wild boars sus scrofa l. of the european and the asian areas of ussr. | the karyotypes of the 80 wild boars of the four subspecies, sus scrofa ussuricus heude from the far east of ussr, s. s. nigripes blanf. from kirghizia (the middle asia), s. s. attila thos. from azerbaijan, s. s. ferus from lithuania, byelorussia and central russia, and the 44 domestic pigs of the five different breeds (vietnamese black, siberian omskaja gray, kakhethian-aborigen georgian, mangalica hungarian, landrace swedish), were studied by the giemsa banding method. differential staining by ... | 1975 | 24419506 |
| detection of antibodies against paracoccidioides brasiliensis in free-range domestic pigs (sus scrofa). | paracoccidioidomycosis, caused by the thermodimorphic fungus paracoccidioides brasiliensis, is a human systemic mycosis prevalent in latin america. paracoccidioidomycosis affects mainly male rural workers, causing granulomatous lesions in several organs such as the lungs, liver and spleen. the participation of other animal species in the fungus epidemiology is not well understood. the objective of this study was to evaluate the infection of free-range domestic pigs by p. brasiliensis. serum samp ... | 2014 | 24436011 |
| immunohistochemical analysis of blood vessels in peri-implant mucosa: a comparison between mini-incision flapless and flap surgeries in domestic pigs. | the aim of this experimental study is to compare the effect of mini-incision flapless versus flap technique of implant placement on the amount of vascular structures and blood vessel elements in peri-implant soft tissue, using immunohistochemical analysis. | 2015 | 24438481 |
| a serological survey for antibodies against foot-and-mouth disease virus (fmdv) in domestic pigs during outbreaks in kenya. | foot-and-mouth disease (fmd) is endemic in kenya and has been well studied in cattle, but not in pigs, yet the role of pigs is recognised in fmd-free areas. this study investigated the presence of antibodies against fmd virus (fmdv) in pigs sampled during a countrywide random survey for fmd in cattle coinciding with sat 1 fmdv outbreaks in cattle. a total of 191 serum samples were collected from clinically healthy pigs in 17 districts. forty-two of the 191 sera were from pigs vaccinated against ... | 2014 | 24442573 |
| domestication and the mitochondrial genome: comparing patterns and rates of molecular evolution in domesticated mammals and birds and their wild relatives. | studies of domesticated animals have led to the suggestion that domestication could have significant effects on patterns of molecular evolution. in particular, analyses of mitochondrial genome sequences from domestic dogs and yaks have yielded higher ratios of non-synonymous to synonymous substitutions in the domesticated lineages than in their wild relatives. these results are important because they imply that changes to selection or population size operating over a short timescale can cause si ... | 0 | 24459286 |
| miniaturized mechanical chest compressor improves calculated cerebral perfusion pressure without compromising intracranial pressure during cardiopulmonary resuscitation in a porcine model of cardiac arrest. | one of the major goals of cardiopulmonary resuscitation (cpr) is to provide adequate oxygen delivery to the brain for minimizing cerebral injury resulted from cardiac arrest. the optimal chest compression during cpr should effectively improve brain perfusion without compromising intracranial pressure (icp). our previous study has demonstrated that the miniaturized mechanical chest compressor improved hemodynamic efficacy and the success of cpr. in the present study, we investigated the effects o ... | 2014 | 24463224 |
| differences in vessel healing following delivery of everolimus or paclitaxel: a comparative experimental study using identical stent and biodegradable polymer platforms. | we aimed to compare the vascular effects exclusive to antiproliferative agents by using identical stent and biodegradable polymeric matrices eluting everolimus (bp-ees) (carlo; balton) and paclitaxel (bp-pes) (luc-chopin2; balton) in the porcine model of coronary injury. a total of 37 stents were implanted with 110% overstretch in the coronary arteries of 14 domestic pigs: 13 bp-pes, 16 bp-ees and eight bare metal stents (bms) (chopin2; balton). coronary angiography was performed after 28 and 90 ... | 2014 | 24469344 |
| assessment of regional ventilation distribution: comparison of vibration response imaging (vri) with electrical impedance tomography (eit). | vibration response imaging (vri) is a bedside technology to monitor ventilation by detecting lung sound vibrations. it is currently unknown whether vri is able to accurately monitor the local distribution of ventilation within the lungs. we therefore compared vri to electrical impedance tomography (eit), an established technique used for the assessment of regional ventilation. | 2014 | 24475160 |
| the role of viral and host micrornas in the aujeszky's disease virus during the infection process. | porcine production is a primary market in the world economy. controlling swine diseases in the farm is essential in order to achieve the sector necessities. aujeszky's disease is a viral condition affecting pigs and is endemic in many countries of the world, causing important economic losses in the swine industry. micrornas (mirnas) are non-coding rnas which modulates gene expression in animals, plants and viruses. with the aim of understanding mirna roles during the aujeszky's disease virus [ad ... | 2014 | 24475202 |
| novel avian-origin influenza a (h7n9) virus attachment to the respiratory tract of five animal models. | we determined the pattern of attachment of the avian-origin h7n9 influenza viruses a/anhui/1/2013 and a/shanghai/1/2013 to the respiratory tract in ferrets, macaques, mice, pigs, and guinea pigs and compared it to that in humans. the h7n9 attachment pattern in macaques, mice, and to a lesser extent pigs and guinea pigs resembled that in humans more closely than the attachment pattern in ferrets. this information contributes to our knowledge of the different animal models for influenza. | 2014 | 24478425 |
| genetic characterization of porcine circovirus type 2 in the korean wild boar population. | the porcine circovirus type 2 (pcv2) has reached very high levels in the pig population in south korea and throughout the world since it was first described in the late 1990s. in this study, we found that the prevalence of pcv2 in the korean wild boar population was 4.98% (91/1825). interestingly, 19 pcv2 orf2 sequences that could be completely sequenced showed that they belonged only to genotype pcv2b, subgroup 1a/b (n=16) and 1c (n=3). we suggest that sites potentially under positive selection ... | 2014 | 24480584 |
| epicardial ablation performance of a novel radiofrequency device on the beating heart in pigs. | only bipolar clamps create reliable transmural lesions on the beating heart. this study evaluated the performance of a new radiofrequency (rf) device on the beating heart in an acute porcine model. | 2014 | 24484804 |
| seroprevalence of hepatitis e virus in domestic pigs and wild boars in switzerland. | hepatitis e is considered an emerging human viral disease in industrialized countries. studies from switzerland report a human seroprevalence of hepatitis e virus (hev) of 2.6-21%, a range lower than in adjacent european countries. the aim of this study was to determine whether hev seroprevalence in domestic pigs and wild boars is also lower in switzerland and whether it is increasing and thus indicating that this zoonotic viral infection is emerging. serum samples collected from 2,001 pigs in 2 ... | 2014 | 24499160 |
| domestic pigs as potential reservoirs of human and animal trypanosomiasis in northern tanzania. | pig keeping is becoming increasingly common across sub-saharan africa. domestic pigs from the arusha region of northern tanzania were screened for trypanosomes using pcr-based methods to examine the role of pigs as a reservoir of human and animal trypanosomiasis. | 2013 | 24499540 |
| screening of cellular proteins that interact with the classical swine fever virus non-structural protein 5a by yeast two-hybrid analysis. | classical swine fever virus (csfv), the pathogen of classical swine fever (csf), causes severe hemorrhagic fever and vascular necrosis in domestic pigs and wild boar. a large number of evidence has proven that non-structural 5a (ns5a) is not only a very important part of viral replication complex, but also can regulate host cell's function; however, the underlying mechanisms remain poorly understood. in the current study, aiming to find more clues in understanding the molecular mechanisms of csf ... | 2014 | 24499791 |
| import risk assessment incorporating a dose-response model: introduction of highly pathogenic porcine reproductive and respiratory syndrome into australia via illegally imported raw pork. | highly pathogenic porcine reproductive and respiratory syndrome (prrs) has spread through parts of south-east asia, posing a risk to australia. the objective of this study was to assess the probability of infection of a feral or domestic pig in australia with highly pathogenic prrs following ingestion of illegally imported raw pork. a conservative scenario was considered in which 500 g of raw pork was imported from the philippines into australia without being detected by border security, then di ... | 2014 | 24502944 |
| genome-wide scans to detect positive selection in large white and tongcheng pigs. | due to the direction, intensity, duration and consistency of genetic selection, especially recent artificial selection, the production performance of domestic pigs has been greatly changed. therefore, we reasoned that there must be footprints or selection signatures that had been left during domestication. in this study, with porcine 60k beadchip genotyping data from both commercial large white and local chinese tongcheng pigs, we calculated the extended haplotype homozygosity values of the two ... | 2014 | 24506146 |
| seroprevalence and correlates of toxoplasma gondii infection in domestic pigs in veracruz state, mexico. | toxoplasma gondii infection in pigs has epidemiological concern for its contributing role in human infections. we determined seroprevalence of t. gondii infection in 402 domestic pigs raised in backyards in veracruz state, mexico using the modified agglutination test (mat; cut off 1:25); 182 (45.3%) of the 402 pigs were seropositive with mat titers of 1:25 in 28, 1:50 in 22, 1:100 in 18, 1:200 in 30, 1:400 in 35, 1:800 in 23, 1:1,600 in 11, and 1:3,200 or higher in 15. seropositive pigs were fou ... | 2014 | 24510223 |
| classical swine fever in europe--the current situation. | classical swine fever (csf) is considered to be one of the most important viral diseases in pigs worldwide. in many parts of the world great efforts are being undertaken to reduce economic losses caused by csf or to eradicate the disease. among the member states of the european union (eu) a harmonized strategy for diagnosis, control and eradication of csf is applied. success of the common strategy is documented by the decreasing number of outbreaks during the last decade. the present article sum ... | 2014 | 24511821 |
| prospects of improved classical swine fever control in backyard pigs through oral vaccination. | success in controlling classical swine fever (csf) in regions with high proportions of pigs kept in small scale and low-biosecurity production systems, often referred to as backyard production, tends to be hampered by the lack of control strategies properly addressing the peculiarities of this epidemiologically important subpopulation. under many circumstances the commonly practiced parenteral immunisation using live attenuated c-strain vaccine shows limitations concerning outreach of services a ... | 2014 | 24511822 |
| abdominal hypertension and decompression: the effect on peritoneal metabolism in an experimental porcine study. | the aim of this study was to investigate the abdominal metabolic response and circulatory changes after decompression of intra-abdominal hypertension in a porcine model. | 2014 | 24530179 |
| characterisation of the active/de-active transition of mitochondrial complex i. | oxidation of nadh in the mitochondrial matrix of aerobic cells is catalysed by mitochondrial complex i. the regulation of this mitochondrial enzyme is not completely understood. an interesting characteristic of complex i from some organisms is the ability to adopt two distinct states: the so-called catalytically active (a) and the de-active, dormant state (d). the a-form in situ can undergo de-activation when the activity of the respiratory chain is limited (i.e. in the absence of oxygen). the m ... | 2014 | 24569053 |
| pathogen presence in feral pigs and their movement around two commercial piggeries in queensland, australia. | feral pigs are wild animal reservoirs of infectious pathogens transmissible to other species, all of which are transmissible to domestic pigs. the objective of this study was to detect the presence of harmful production-limiting pathogens; brucella suis, leptospira species, lawsonia intracellularis, mycoplasma hyopneumoniae and actinobacillus pleuropneumoniae in a feral pig population within a 10 km radius of two large-scale commercial piggeries in southern queensland, australia. the movement pa ... | 2014 | 24572722 |
| novel emboli protection cannula during cardiac surgery: first animal study. | stroke after open heart surgery is a major cause of morbidity and mortality. up to 60% of intraoperative cerebral events are caused by emboli generated by manipulations of the aorta during surgery. this is the first animal study evaluating the safety and efficacy of a novel aortic cannula designed to extract solid and gaseous emboli during cardiac surgery. | 2014 | 24585639 |
| effects of amlodipine and perindoprilate on the structure and function of mitochondria in ventricular cardiomyocytes during ischemia-reperfusion in the pig. | the aim of this study was to determine whether amlodipine and/or perindoprilate injected intravenously (iv) prior to ischemia exerted protective effects on mitochondria structural and functional alterations induced by ischemia and aggravated by reperfusion. heart rate, the duration of monophasic action potentials (dmap), peak of the time derivative of left ventricular pressure (lv dp/dt max), mitochondria structural and functional parameters in the left ventricle ischemic area were measured afte ... | 2015 | 24588464 |
| origin of porcine circovirus type 2 (pcv2) from swine affected by pcv2-associated diseases in croatia. | porcine circovirus type 2 (pcv2) causes some of the most significant economic losses in pig production. several multisystemic syndromes have been attributed to pcv2 infection, which are known as pcv2-associated diseases (pcvds). this study investigated the origin and evolution of pcv2 sequences in domestic pigs and wild boars affected by pcvds in croatia. viral sequences were recovered from three wild boars diagnosed with pcv2-systemic disease (pcv2-sd), 63 fetuses positive for pcv2 dna as deter ... | 2014 | 24591478 |
| comparison of self-efficacy and its improvement after artificial simulator or live animal model emergency procedure training. | the objective of this study is to compare post-training self-efficacy between artificial simulators and live animal training for the performance of emergency medical procedures. volunteer airmen of the 81st medical group, without prior medical procedure training, were randomly assigned to two experimental arms consisting of identical lectures and training of diagnostic peritoneal lavage, thoracostomy (chest tube), and cricothyroidotomy on either the traumaman (simulab corp., seattle, washington) ... | 2014 | 24594468 |
| lapita diet in remote oceania: new stable isotope evidence from the 3000-year-old teouma site, efate island, vanuatu. | remote oceania was colonized ca. 3000 bp by populations associated with the lapita cultural complex, marking a major event in the prehistoric settlement of the pacific islands. although over 250 lapita sites have been found throughout the western pacific, human remains associated with lapita period sites are rare. the site of teouma, on efate island, vanuatu has yielded the largest burial assemblage (n=68 inhumations) of lapita period humans ever discovered, providing a unique opportunity for as ... | 2014 | 24598939 |
| isolation and pathogenicity of toxoplasma gondii in naturally infected (rustic farm) pigs in southern brazil. | this study reported a serological test for toxoplasma gondii infection in 100 pigs from 58 rural farms in the state of rio grande do sul, brazil. thirty-six pigs were seropositive (ifat≥1:64). bioassays were performed for all 36 seropositive pigs, and 17 isolates were obtained (47.2%). seven of these isolates (41.2%) were highly pathogenic to mice, as clinical signs of acute infection were observed, and tachyzoites were found in the peritoneal exudates, livers, and lungs. the remaining 10 isolat ... | 2014 | 24612744 |
| a pro23his mutation alters prenatal rod photoreceptor morphology in a transgenic swine model of retinitis pigmentosa. | functional studies have detected deficits in retinal signaling in asymptomatic children from families with inherited autosomal dominant retinitis pigmentosa (rp). whether retinal abnormalities are present earlier during gestation or shortly after birth in a subset of children with autosomal dominant rp is unknown and no appropriate animal rp model possessing visual function at birth has been available to examine this possibility. in a recently developed transgenic p23h (tgp23h) rhodopsin swine m ... | 2014 | 24618321 |
| cone photoreceptors develop normally in the absence of functional rod photoreceptors in a transgenic swine model of retinitis pigmentosa. | human and swine retinas have morphological and functional similarities. in the absence of primate models, the swine is an attractive model to study retinal function and disease, with its cone-rich visual streak, our ability to manipulate their genome, and the differences in susceptibility of rod and cone photoreceptors to disease. we characterized the normal development of cone function and its subsequent decline in a p23h rhodopsin transgenic (tgp23h) miniswine model of autosomal dominant rp. | 2014 | 24618325 |
| black cobra (naja naja karachiensis) lysates exhibit broad-spectrum antimicrobial activities. | it is hypothesized that animals living in polluted environments possess antimicrobials to counter pathogenic microbes. the fact that snakes feed on germ-infested rodents suggests that they encounter pathogenic microbes and likely possess antimicrobials. the venom is used only to paralyze the rodent, but the ability of snakes to counter potential infections in the gut due to disease-ridden rodents requires robust action of the immune system against a broad range of pathogens. to test this hypothe ... | 2014 | 24625321 |
| prenatal stress produces anxiety prone female offspring and impaired maternal behaviour in the domestic pig. | numerous studies have shown that prenatal stress (pns) can have profound effects on postnatal well-being. here, the domestic pig (sus scrofa) was used to investigate pns effects owing to the direct relevance for farm animal welfare and the developing status of the pig as a large animal model in translational research. pregnant primiparous sows were exposed, in mid-gestation, to either a social stressor (mixing with unfamiliar conspecifics) or were kept in stable social groups. the ratio of level ... | 2014 | 24631303 |
| comamonas faecalis sp. nov., isolated from domestic pig feces. | a new bacterial strain, designated as ff42(t), was isolated from feces of domestic pigs-collected from suwon, korea-and was characterized to determine its taxonomic position. strain ff42(t) was observed to be gram negative, aerobic, non-spore forming, motile, and rod-shaped cells. based on the phylogenetic and 16s rrna sequence analyses, it was revealed that strain ff42(t) belonged to the genus comamonas. the highest degree of sequence similarities was determined to be with comamonas zonglianii ... | 2014 | 24633862 |
| the influence of bicuspid aortic valves on the dynamic pressure distribution in the ascending aorta: a porcine ex vivo model. | the aim of the study was to simulate the effect of different bicuspid aortic valve configurations on the dynamic pressure distribution in the ascending aorta. | 2014 | 24644312 |
| xenoantibody response to porcine islet cell transplantation using gtko, cd55, cd59, and fucosyltransferase multiple transgenic donors. | promising developments in porcine islet xenotransplantation could resolve the donor pancreas shortage for patients with type 1 diabetes. using α1,3-galactosyltransferase gene knockout (gtko) donor pigs with multiple transgenes should extend xenoislet survival via reducing complement activation, thrombus formation, and the requirement for exogenous immune suppression. studying the xenoantibody response to gtko/hcd55/hcd59/hht islets in the pig-to-baboon model, and comparing it with previously ana ... | 2014 | 24645827 |
| microsporidia detection and genotyping study of human pathogenic e. bieneusi in animals from spain. | microsporidia are ubiquitous parasites infecting all animal phyla and we present evidence that supports their zoonotic potential. fecal samples taken from domestic (cats and dogs), farm (pigs, rabbits and ostriches) and wild animals (foxes) from different provinces of spain were evaluated for microsporidia infection by light microscopy and pcr. after microsporidia species identification, e. bieneusi genotypes were additionally studied by sequence analysis of the its region. eighty-five samples o ... | 2014 | 24651457 |
| the impact of csfv on the immune response to control infection. | the severity of the acute form of csf is responsible for the high mortality rate and has been the subject of many studies. nevertheless, some animals are likely to develop a mild, chronic, or unapparent form of the disease. paradoxically, this clinical form of the disease has not been well studied, especially regarding its pathogenesis. in this study, we investigated the infection in domestic pigs that is caused by the csfv cat01 strain, which is responsible for the 2001-2002 csfv outbreak in ca ... | 2014 | 24657786 |
| porcine skin damage thresholds for 0.6 to 9.5 cm beam diameters from 1070-nm continuous-wave infrared laser radiation. | there is an increasing use of high-power fiber lasers in manufacturing and telecommunications industries operating in the infrared spectrum between 1000 and 2000 nm, which are advertised to provide as much as 10 kw continuous output power at 1070 nm. safety standards have traditionally been based on experimental and modeling investigations with scant data available for these wavelengths. a series of studies using 1070-nm infrared lasers to determine the minimum visible lesion damage thresholds i ... | 2014 | 24658776 |
| ophthalmic artery microcatheterization for research purposes in pigs. a technical note. | to describe a technique for micro catheterization of the external ophthalmic artery (eo) in pigs for investigational and training purposes. | 2014 | 24660750 |
| animal study assessing safety of an acoustic coupling fluid that holds the potential to avoid surgically induced artifacts in 3d ultrasound guided operations. | use of ultrasound in brain tumor surgery is common. the difference in attenuation between brain and isotonic saline may cause artifacts that degrade the ultrasound images, potentially affecting resection grades and safety. our research group has developed an acoustic coupling fluid that attenuates ultrasound energy like the normal brain. we aimed to test in animals if the newly developed acoustic coupling fluid may have harmful effects. | 2014 | 24666721 |
| medieval horse stable; the results of multi proxy interdisciplinary research. | a multi proxy approach was applied in the reconstruction of the architecture of medieval horse stable architecture, the maintenance practices associated with that structure as well as horse alimentation at the beginning of 13th century in central europe. finally, an interpretation of the local vegetation structure along morava river, czech republic is presented. the investigated stable experienced two construction phases. the infill was well preserved and its composition reflects maintenance pra ... | 2014 | 24670874 |
| segmented filamentous bacteria: commensal microbes with potential effects on research. | segmented filamentous bacteria (sfb) are commensal bacteria that were first identified in the ilea of mice and rats. morphologically similar bacteria occur in a broad range of host species, but all strains have been refractory to in vitro culture thus far. although sfb were once considered innocuous members of the intestinal microbiota of laboratory rodents, they are now known to affect the development of the immune system in rodents and, subsequently, the phenotype of models of both enteric and ... | 0 | 24674582 |
| genome-wide association studies for hematological traits in chinese sutai pigs. | it has been shown that hematological traits are strongly associated with the metabolism and the immune system in domestic pig. however, little is known about the genetic architecture of hematological traits. to identify quantitative trait loci (qtl) controlling hematological traits, we performed single marker genome-wide association studies (gwas) and haplotype analysis for 15 hematological traits in 495 chinese sutai pigs. | 2014 | 24674592 |
| experimental schmallenberg virus infection of pigs. | schmallenberg virus (sbv) is a newly emerged virus responsible for an acute non-specific syndrome in adult cattle including high fever, decrease in milk production and severe diarrhea. it also causes reproductive problems in cattle, sheep and goat including abortions, stillbirths and malformations. the role of pigs in the epidemiology of sbv has not yet been evaluated while this could be interesting seen their suggested role in the epidemiology of the closely related akabane virus. to address th ... | 2014 | 24679959 |
| glued versus stapled anastomosis of the colon: an experimental study to determine comparative resistance to intraluminal pressure. | this study compared the resistance of glued versus stapled anastomosis of the colon to intraluminal pressures at different times during healing. | 2014 | 24685174 |
| porcine salivary analysis by 2-dimensional gel electrophoresis in 3 models of acute stress: a pilot study. | the purpose of this research was to study changes in the salivary proteome of healthy pigs in stressful situations to identify any potential new salivary biomarker of stress. three groups of animals were subjected to 3 stress models: snaring restraint followed by simulated sampling of vena cava blood; brief transport by road; and restriction of movement in a digestibility cage. saliva was obtained from each animal before and 15 and 30 min after the induction of stress. the samples from the anima ... | 0 | 24688174 |
| law of cooling, heat conduction and stefan-boltzmann radiation laws fitted to experimental data for bones irradiated by co2 laser. | the rate of cooling of domesticated pig bones is investigated within the temperature range of 20°c-320°c. within the afore-mentioned temperature range, it was found that different behaviors in the rate of cooling were taking place. for bones reaching a temperature within the lower temperature range of 20°c-50°c, it was found that the rate of cooling is mostly governed by the empirical newton's law of cooling. it is also shown that a transition is taking place somewhere within 50°c-100°c, where b ... | 2014 | 24688807 |
| variation in mitochondrial minichromosome composition between blood-sucking lice of the genus haematopinus that infest horses and pigs. | the genus haematopinus contains 21 species of blood-sucking lice, parasitizing both even-toed ungulates (pigs, cattle, buffalo, antelopes, camels and deer) and odd-toed ungulates (horses, donkeys and zebras). the mitochondrial genomes of the domestic pig louse, haematopinus suis, and the wild pig louse, haematopinus apri, have been sequenced recently; both lice have fragmented mitochondrial genomes with 37 genes on nine minichromosomes. to understand whether the composition of mitochondrial mini ... | 2014 | 24690192 |
| characterization of porcine nlrp3 inflammasome activation and its upstream mechanism. | inflammasomes, which are intracellular sensors of endogenous or exogenous danger signals, activate caspase-1, resulting in interleukin (il)-1β maturation. although most studies on inflammasomes have been performed in human and/or mouse-derived macrophages, porcine inflammasome activation has not been elucidated even though pigs are considered one of the best animal models for translational and preclinical investigations. in this study, we optimized detection of porcine il-1β secretion, which is ... | 2014 | 24691785 |
| experimental feeding of hydrilla verticillata colonized by stigonematales cyanobacteria induces vacuolar myelinopathy in painted turtles (chrysemys picta). | vacuolar myelinopathy (vm) is a neurologic disease primarily found in birds that occurs when wildlife ingest submerged aquatic vegetation colonized by an uncharacterized toxin-producing cyanobacterium (hereafter "ucb" for "uncharacterized cyanobacterium"). turtles are among the closest extant relatives of birds and many species directly and/or indirectly consume aquatic vegetation. however, it is unknown whether turtles can develop vm. we conducted a feeding trial to determine whether painted tu ... | 2014 | 24695109 |
| optical nerve identification in head and neck surgery after er:yag laser ablation. | facial nerve function may be hampered by iatrogenic damage during head and neck laser surgery procedures. optical techniques can serve as a basis for feedback-controlled tissue-specific laser surgery on the jaw bone and the parotid gland. in order to preserve nerve tissue during laser surgery, the alteration of optical tissue properties through laser-tissue interactions have to be taken into account. it was the aim of this study to evaluate the viability of optical tissue differentiation through ... | 2014 | 24696381 |
| identification of g protein-coupled estrogen receptor in human and pig spermatozoa. | estrogens are known to influence functional properties of mammalian spermatozoa inducing rapid responses through the classical estrogen receptors (erα and erβ). recently, the g protein-coupled estrogen receptor (gper) has been identified as mediator of fast non-genomic estrogen effects in different cells. this work investigated the expression of gper in human and pig spermatozoa using immunofluorescence, western blot analysis and rt-pcr. gper was found to be confined to the mid-piece of human sp ... | 2014 | 24697543 |
| novel rope-based sampling of classical swine fever shedding in a group of wild boar showing low contagiosity upon experimental infection with a classical swine fever field strain of genotype 2.3. | several classical swine fever (csf) epidemics in wild boar and domestic pigs in europe during the last decades have been caused by csf virus (csfv) strains of genotype 2.3. this genotype is known to be virulent leading to high morbidity and mortality. we experimentally infected two eight months old wild boar with 10(5,5) tcid50 of csfv genotype 2.3 and kept the animals together with five noninoculated wild boar of the same age. our original purpose was to evaluate a non-invasive sampling method ... | 2014 | 24698133 |
| the porcine innate immune system: an update. | over the last few years, we have seen an increasing interest and demand for pigs in biomedical research. domestic pigs (sus scrofa domesticus) are closely related to humans in terms of their anatomy, genetics, and physiology, and often are the model of choice for the assessment of novel vaccines and therapeutics in a preclinical stage. however, the pig as a model has much more to offer, and can serve as a model for many biomedical applications including aging research, medical imaging, and pharm ... | 2014 | 24709051 |
| whole-genome sequencing of berkshire (european native pig) provides insights into its origin and domestication. | domesticated organisms have experienced strong selective pressures directed at genes or genomic regions controlling traits of biological, agricultural or medical importance. the genome of native and domesticated pigs provide a unique opportunity for tracing the history of domestication and identifying signatures of artificial selection. here we used whole-genome sequencing to explore the genetic relationships among the european native pig berkshire and breeds that are distributed worldwide, and ... | 2014 | 24728479 |
| decomposition odour profiling in the air and soil surrounding vertebrate carrion. | chemical profiling of decomposition odour is conducted in the environmental sciences to detect malodourous target sources in air, water or soil. more recently decomposition odour profiling has been employed in the forensic sciences to generate a profile of the volatile organic compounds (vocs) produced by decomposed remains. the chemical profile of decomposition odour is still being debated with variations in the voc profile attributed to the sample collection technique, method of chemical analy ... | 2014 | 24740412 |
| assessment of renal artery stenosis using intravoxel incoherent motion diffusion-weighted magnetic resonance imaging analysis. | diffusion-weighted magnetic resonance imaging is a powerful tool to assess renal morphology. however, its quantitative index, apparent diffusion coefficient (adc), derived from a conventional monoexponential model can vary with both functional and structural alterations as well as the choice of b values. in contrast, the intravoxel incoherent motion (ivim) biexponential model provides independent parameters that may represent broader aspects of renal pathophysiology. we hypothesized that ivim an ... | 2014 | 24743589 |
| seroprevalence of hepatitis e virus (hev) in humans living in high pig density areas of germany. | an increase in acute autochthonous hepatitis e virus (hev) infections has been recorded in germany. these are suspected to be zoonotically transmitted from wild boar, deer and domestic pig. the latter may represent a major reservoir for hev. in this study, 537 sera from humans living in westphalia and lower saxony, representing areas of high pig density in germany, were tested for the presence of hev-specific antibodies. among them were 302 individuals with occupational, direct contact to pigs a ... | 2014 | 24744181 |
| experimental ex vivo traumatic intrusion in the mandibular incisors of the farm pig, sus scrofa. | traumatic intrusion of incisor teeth occurs frequently in young children, as well as in teens and adults; however, the biological mechanisms promoting negative sequelae or recovery are not well understood (oral surg oral med oral pathol oral radiol endod 2009;107:493 and vital health stat 11 2007;248:1). modeling intrusive trauma and post-traumatic healing in an animal model offers the opportunity to define these biological mechanisms and to inform the design of treatments. the objective of this ... | 2014 | 24751110 |
| valsartan regulates myocardial autophagy and mitochondrial turnover in experimental hypertension. | renovascular hypertension alters cardiac structure and function. autophagy is activated during left ventricular hypertrophy and linked to adverse cardiac function. the angiotensin ii receptor blocker, valsartan, lowers blood pressure and is cardioprotective, but whether it modulates autophagy in the myocardium is unclear. we hypothesized that valsartan would alleviate autophagy and improve left ventricular myocardial mitochondrial turnover in swine renovascular hypertension. domestic pigs were r ... | 2014 | 24752430 |
| evaluation of genetic introgression from domesticated pigs into the ryukyu wild boar population on iriomote island in japan. | we evaluated genetic introgression from domesticated pigs into the ryukyu wild boar (rwb) population on iriomote island based on their genetic structure and diversity. we used a combination of mitochondrial dna d-loop region (596 bp) polymorphisms and 23 microsatellite markers. rwbs (n = 130) were collected from 18 locations on iriomote island and compared with 66 reference samples of european and asian domestic pigs. we identified six distinct haplotypes, involving 22 single nucleotide polymorp ... | 2014 | 24754898 |
| discovering up-regulated vegf-c expression in swine umbilical vein endothelial cells by classical swine fever virus shimen. | infection of domestic swine with the highly virulent shimen strain of classical swine fever virus causes hemorrhagic lymphadenitis and diffuse hemorrhaging in infected swine. we analyzed patterns of gene expression for csfv shimen in swine umbilical vein endothelial cells (suvecs). transcription of the vascular endothelial growth factor (vegf) c gene (vegf-c) and translation of the corresponding protein were significantly up-regulated in suvecs. our findings suggest that vegf-c is involved in me ... | 2014 | 24758593 |
| the origin of bacteria responsible for bioerosion to the internal bone microstructure: results from experimentally-deposited pig carcasses. | it is unclear whether the principal forms of bioerosion that are often found within the internal microstructure of human bone are produced by intrinsic gut microbiota or exogenous bacteria from the soil. the aim of this study was to attempt to resolve this issue through the histological analysis of bone sampled from experimentally-deposited domestic pig (sus scrofa) carcasses. confirmation of either scenario will dictate how patterns of bone bioerosion can be used in reconstructions of taphonomi ... | 2014 | 24763128 |
| preclinical randomised safety, efficacy and physiologic study of the silicon dioxide inert-coated axetis and bare metal stent: short-, mid- and long-term outcome. | to evaluate the short-, mid- and long-term safety, efficacy and vascular physiology of axetis silicon dioxide (sio2, abrading the micropores) inert-coated stent implantation in a randomised preclinical setting. | 2015 | 24769439 |
| complete sequencing and phylogenetic analysis of porcine kobuvirus in domestic pigs in northwest china. | porcine kobuvirus, a member of the genus kobuvirus that is associated with diarrhea, has been reported in many countries. we determined the complete genome sequence and investigated the genetic evolution of the kobuvirus strain swkov ch441, which was detected in the highland of gansu province in northwest china. the viral genome is 8149 nucleotides (nt) long, including a 29-nt poly(a) tail of the 3' end, and is 90 nt shorter in the 2b coding region than those of other kobuvirus strains whose seq ... | 2014 | 24777826 |
| inflammation blood and tissue factors of plaque growth in an experimental model evidenced by a systems approach. | the multifactorial pathogenesis of coronary atherosclerotic lesion formation has been investigated in a swine model of high cholesterol diet induced atherogenesis and data processed by a systems approach. | 2014 | 24778640 |
| detecting mitochondrial signatures of selection in wild tibetan pigs and domesticated pigs. | selection in genomic regions is prevalent in mammals; however, the effects of selection on the mitogenome are not clearly understood. we determined the complete mitochondrial dna (mtdna) sequences from six wild tibetan pigs from the tibetan plateau and four domestic pig breeds from the lowland of neighboring southwest china. nucleotide diversity analysis using the sliding window method showed that the nucleotide diversity of wild tibetan pigs in most regions of the mitogenome was higher than tha ... | 2016 | 24810064 |
| assessment of fetal cell chimerism in transgenic pig lines generated by sleeping beauty transposition. | human cells migrate between mother and fetus during pregnancy and persist in the respective host for long-term after birth. fetal microchimerism occurs also in twins sharing a common placenta or chorion. whether microchimerism occurs in multiparous mammals such as the domestic pig, where fetuses have separate placentas and chorions, is not well understood. here, we assessed cell chimerism in litters of wild-type sows inseminated with semen of transposon transgenic boars. segregation of three ind ... | 2014 | 24811124 |
| technical development of a new semispherical radiofrequency bipolar device (ronja): ex vivo and in vivo studies. | the aim of this study is to inform about the development of a new semispherical surgical instrument for the bipolar multielectrode radiofrequency liver ablation. present tools are universal; however they have several disadvantages such as ablation of healthy tissue, numerous needle punctures, and, therefore, longer operating procedure. our newly designed and tested semispherical surgical tool can solve some of these disadvantages. by conducting an in vivo study on a set of 12 pigs, randomly divi ... | 2014 | 24812620 |
| repeated evolution of chimeric fusion genes in the β-globin gene family of laurasiatherian mammals. | the evolutionary fate of chimeric fusion genes may be strongly influenced by their recombinational mode of origin and the nature of functional divergence between the parental genes. in the β-globin gene family of placental mammals, the two postnatally expressed δ- and β-globin genes (hbd and hbb, respectively) have a propensity for recombinational exchange via gene conversion and unequal crossing-over. in the latter case, there are good reasons to expect differences in retention rates for the re ... | 2014 | 24814285 |
| establishing a critical-size mandibular defect model in growing pigs: characterization of spontaneous healing. | a large animal model is desired for preclinical studies aimed at reconstructing severe mandibular skeletal defects using tissue engineering techniques. to identify the size and location requirements for a mandibular critical-size bone defect in growing pigs, the present study investigated the spontaneous healing of surgically created mandibular defects. | 2014 | 24815793 |
| identification of blood meal sources of lutzomyia longipalpis using polymerase chain reaction-restriction fragment length polymorphism analysis of the cytochrome b gene. | an analysis of the dietary content of haematophagous insects can provide important information about the transmission networks of certain zoonoses. the present study evaluated the potential of polymerase chain reaction-restriction fragment length polymorphism (pcr-rflp) analysis of the mitochondrial cytochrome b (cytb) gene to differentiate between vertebrate species that were identified as possible sources of sandfly meals. the complete cytb gene sequences of 11 vertebrate species available in ... | 2014 | 24821056 |
| blood-banking techniques for plateletpheresis in swine. | during the past several years, trauma resuscitation in human patients has evolved from decreased use of crystalloids to increased use of blood products. of high interest is the role of platelets in trauma resuscitation. because conducting prehos- pital resuscitation in human trauma patients is very difficult, swine are often the animal model of choice for such studies because their coagulation and hemodynamic systems are similar to those in humans. however, consistent production of sufficient sw ... | 0 | 24827574 |
| using biplanar fluoroscopy to guide radiopaque vascular injections: a new method for vascular imaging. | studying vascular anatomy, especially in the context of relationships with hard tissues, is of great interest to biologists. vascular studies have provided significant insight into physiology, function, phylogenetic relationships, and evolutionary patterns. injection of resin or latex into the vascular system has been a standard technique for decades. there has been a recent surge in popularity of more modern methods, especially radiopaque latex vascular injection followed by ct scanning and dig ... | 2014 | 24835249 |
| phylogenetic comparison of porcine circovirus type 2 (pcv2) and porcine reproductive respiratory syndrome virus (prrsv) strains detected in domestic pigs until 2008 and in 2012 in croatia. | porcine circovirus type 2 (pcv2) and porcine reproductive and respiratory syndrome virus (prrsv) have been present for the last 2 decades in croatia, causing large economical losses in the pig production. the clinical features of the infections are mostly manifested by the development of respiratory problems, weight loss and poor growth performance, as well as reproductive failure in pregnant sows. even though the infections are continuously recognized in some regions in croatia, the heterogenei ... | 2014 | 24839544 |
| coexistence of multiple strains of porcine parvovirus 2 in pig farms. | the porcine parvovirus 2 (ppv2) genome was first identified in 2001 in myanmar. recently, the ppv2 genome has been found in several other countries. in this study, the prevalence of ppv2 in japanese domestic pigs was investigated and found to be 58% (69/120) in healthy domestic pigs and 100% (69/69) in sick domestic pigs. sequencing and phylogenetic analysis of the pcr products of the vp1 gene and an almost full length ppv2 clone indicated that diverged ppv2 strains exist in japan. clearly disti ... | 2014 | 24845822 |
| origins and neurochemical complexity of preganglionic neurons supplying the superior cervical ganglion in the domestic pig. | the superior cervical ganglion (scg) is a center of sympathetic innervation of all head and neck organs. scg sympathetic preganglionic neurons (spn) were found in the nucleus intermediolateralis pars principalis (imlpp), the nucleus intermediolateralis pars funicularis (imlpf), the nucleus intercalatus spinalis (ic), and the nucleus intercalatus spinalis pars paraependymalis (icpe). despite its importance, little is known of scg innervation and chemical coding in the laboratory pig, a model that ... | 2015 | 24854048 |
| untangling the hybrid nature of modern pig genomes: a mosaic derived from biogeographically distinct and highly divergent sus scrofa populations. | the merging of populations after an extended period of isolation and divergence is a common phenomenon, in natural settings as well as due to human interference. individuals with such hybrid origins contain genomes that essentially form a mosaic of different histories and demographies. pigs are an excellent model species to study hybridization because european and asian wild boars diverged ~1.2 mya, and pigs were domesticated independently in europe and asia. during the industrial revolution in ... | 2014 | 24863459 |
| safety and efficacy of different paclitaxel-eluting balloons in a porcine model. | paclitaxel-eluting balloons have shown high antiproliferative efficacy in the treatment and prevention of restenosis. nevertheless, not all available devices are equally effective, which makes it interesting to compare results in a preclinical model. our objective was to assess the preclinical efficacy and safety of different devices. | 2014 | 24863594 |
| population phylogenomic analysis and origin of mitochondrial dna in chinese domestic pig. | the genetic diversity of eight domestic pigs was analyzed using a hypervariable fragment in the mitochondrial (mt) dna control region; a portion of the hypervariable control region (515 bp) was sequenced from 153 samples. haplotype diversity and nucleotide diversity in yushan black pig populations were significantly higher than other populations (p < 0.01). a neighbor-joining tree was constructed from domestic pig mtdna and five wild pigs. the results indicate that there are only small differenc ... | 2016 | 24865908 |
| complete genome sequence of classical swine fever virus genotype 2.2 strain bergen. | the complete genome sequence of the genotype 2.2 classical swine fever virus strain bergen has been determined; this strain was originally isolated from persistently infected domestic pigs in the netherlands and is characterized to be of low virulence. | 2014 | 24874676 |
| scabies mites alter the skin microbiome and promote growth of opportunistic pathogens in a porcine model. | the resident skin microbiota plays an important role in restricting pathogenic bacteria, thereby protecting the host. scabies mites (sarcoptes scabiei) are thought to promote bacterial infections by breaching the skin barrier and excreting molecules that inhibit host innate immune responses. epidemiological studies in humans confirm increased incidence of impetigo, generally caused by staphylococcus aureus and streptococcus pyogenes, secondary to the epidermal infestation with the parasitic mite ... | 2014 | 24875186 |
| rational intraabdominal pressure monitoring: how to do it? | intraabdominal hypertension (iah) is increasingly appreciated by intensivists as an important cause of organ dysfunction, even at pressure levels which were previously thought to be harmless. therefore, the goal of this review is to describe the different methods commonly used in clinical practice for intraabdominal pressure (iap) measurement, the advised methodology for each measurement method, and finally to give a rational approach for iap monitoring in daily clinical practice. | 2007 | 24881697 |
| newborn pig trachea cell line cultured in air-liquid interface conditions allows a partial in vitro representation of the porcine upper airway tissue. | the domestic pig is an excellent animal model to study human microbial diseases due to its similarity to humans in terms of anatomy, physiology, and genetics. we assessed the suitability of an in vitro air-liquid interface (ali) culture system for newborn pig trachea (nptr) cells as a practical tool for analyzing the immune response of respiratory epithelial cells to aggressors. this cell line offers a wide microbial susceptibility spectrum to both viruses and bacteria. the purpose of our study ... | 2014 | 24885012 |
| genome-wide identification of long intergenic noncoding rna genes and their potential association with domestication in pigs. | thousands of long intergenic noncoding rnas (lincrnas) have been identified in the human and mouse genomes, some of which play important roles in fundamental biological processes. the pig is an important domesticated animal, however, pig lincrnas remain poorly characterized and it is unknown if they were involved in the domestication of the pig. here, we used available rna-seq resources derived from 93 samples and expressed sequence tag data sets, and identified 6,621 lincrna transcripts from 4, ... | 2014 | 24891613 |