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carp myogens of white and red muscles. gross isolation on sephadex columns of the low-molecular-weight components and examination of their participation in anaerobic glycogenolysis.1. the combined low-molecular-weight protein components of the myogens from carp white and red muscles [about 30% (w/w) of the myogen proteins] have been isolated by gel filtration on sephadex g-75 columns. 2. the presence in this fraction from myogen of white muscle of the three main electrophoretic components previously isolated has been confirmed, and the low molecular weight of the fourth component has been definitely established. 3. the exclusive presence of this fourth component in the myo ...196514343120
carp myogens of white and red muscles. general composition and isolation of low-molecular-weight components of abnormal amino acid composition.1. the general composition of the carp myogens of white and red muscles was examined by electrophoresis and ultracentrifugation. 2. eight and nine peaks were found in the electrophoretic analysis at ph7.3 and i0.075 of white and red muscle respectively. lowering of the ph to 5 or 6 did not increase the number of peaks. the electrophoretic pattern of white-muscle myogen was remarkably different from that of red-muscle myogen, though ultracentrifugal analyses of the both types of myogen gave simil ...196514343157
carp myogens of white and red muscles. properties and amino acid composition of the main low-molecular-weight components of white muscle.1. the three main components of the 1.5-2s ultracentrifugal peak of carp myogen (white muscle) have been isolated by ammonium sulphate fractionation and zone electrophoresis, and crystallized. 2. the molecular weights of these three proteins were determined by sedimentation and diffusion, by the archibald method and by amino acid analysis, and found to lie between 9000 and 13000. 3. their complete amino acid compositions were determined by column chromatography and by their ultraviolet spectra. ...196514343158
[carp parasities in fish farms of the ukraine regions neighboring with poland]. 196414346924
[the dynamics of cysticercus dilepodis campylancistrotae (aubert) infection of the breeding carp (cyprinus carpio l.)]. 196414346927
[changes in the intestine of the carp (cyprinus carpio l.) caused by cysticercus dilepidis campylancistrotae (aubert)]. 196414346928
[physiologic degeneration and restoration of neurosecretory cells of the nucleus praeopticus in carp and in cyprinus carpio]. 195414365599
[neurosecretory function of ganglion cells of the hypothalamic nuclei in esox lucius and cyprinus carpio and its relation to seasonal manifestations of gonadotropic function of the pituitary]. 195514380138
on the ultrastructure of the carp erythrocyte. 195514380202
[studies on neuroglia in cyprinus carpio]. 195514391446
[contribution to the study of myogens of the carp and the plaice]. 196014401109
[effect of the erythropoietic factor isolated from sheep plasma on the erythropoiesis in carps (cyprinus carpio l.)]. 196014412362
[influence of motor agitation on the content of corticosteroids of plasma in a teleost: the carp (cyprinus carpio l.)]. 200814415548
utilization of saccharose solutins and modifications of glycemia in the carp (cyprinus carpio l.) 196014444409
stero-bile acids and bile sterols. xlvi. isolation of a new bile sterol, 3alpha,7alpha,12alpha-trihydroxy-26,27-epoxycholestane from carp bile. 196214449174
[on hygienic evaluation of the accumulations of radioactive iodine by fresh-water fish (year-old carp)]. 196214466758
[increased phagocytic activity in carp leukocytes under the influence of levomycetin]. 196214478715
another activity for the cardiac biologist: carp fishing. 200314499851
beta-adrenergic stimulation induces cardiac ankyrin repeat protein expression: involvement of protein kinase a and calmodulin-dependent kinase.the cardiac ankyrin repeat protein (carp), a nuclear transcription co-factor that negatively regulates cardiac gene expression, is increased in human heart failure and in animal models of cardiac hypertrophy. the mechanism by which carp expression is regulated and the consequences of carp overexpression on cardiac contractility are unknown.200314499857
arteriogenesis is associated with an induction of the cardiac ankyrin repeat protein (carp).collateral artery growth (arteriogenesis) can be induced in rabbit and mice by occlusion of the femoral artery. we aimed to identify genes that are differentially expressed during arteriogenesis.200314499858
effects of chronic hypoxia on inward rectifier k(+) current ( i(k1)) in ventricular myocytes of crucian carp (carassius carassius) heart.some ectothermic vertebrates show unusually good tolerance to oxygen shortage and it is therefore assumed that they might, as a defense mechanism, decrease number or activity of ion channels in order to reduce membrane leakage and thereby atp-dependent ion pumping in hypoxia. although several studies have provided indirect evidence in favor of this 'channel arrest' hypothesis, only few experiments have examined activity of ion channels directly from animals exposed to chronic hypoxia or anoxia i ...200314502436
expression of fmrfamide gene encoded peptides by identified neurons in embryos and juveniles of the pulmonate snail lymnaea stagnalis.immunoreactivity to antibodies (abs) against fmrfamide, carp, and three fmrfamide gene encoded peptides, i.e., eflriamide, the non-fmrfamide peptide "seeply," and the 35-amino-acid "acidic peptide," were investigated in developing embryos and juveniles of lymnaea stagnalis. five early transient embryonic neurons revealed immunoreactivity to eflriamide. one of the early neurons, the central posterior, also expressed seeply and carp immunoreactivity. two neurons in the anlage of the left and right ...200314505033
effects of gallium on common carp (cyprinus carpio): acute test, serum biochemistry, and erythrocyte morphology.gallium (ga) is one of the intermetallic elements that are increasingly being used in making high-speed semiconductors such as gallium arsenide. the purposes of this study were to investigate the effects of gallium on acute toxicity, on serum biochemical variables as well as on erythrocyte morphological changes in the blood stream of common carp (cyprinus carpio). median lethal concentrations were determined in acute tests. the 96-h lc50 value was 19.78 (18.49-21.16) mgl(-1). common carp were ex ...200314505709
increased efficacy of immersion vaccination in fish with hyperosmotic pretreatment.immersion vaccination is common practice in aquaculture, because of its convenience for mass vaccination with sufficient protection. however, the mechanisms of antigen uptake and presentation, resulting in a protective immune response and the role of the innate immune system therein are largely unknown. the impact of immersion vaccination on fish physiology and on the ensuing innate and specific immune response was characterized with fluorescently labeled particulate and soluble model antigens. ...200314505897
active/de-active transition of respiratory complex i in bacteria, fungi, and animals.mammalian complex i (nadh:ubiquinone oxidoreductase) exists as a mixture of interconvertible active (a) and de-activated (d) forms. the a-form is capable of nadh:quinone-reductase catalysis, but not the d-form. complex i from the bacterium paracoccus denitrificans, by contrast, exists only in the a-form. this bacterial complex contains 32 fewer subunits than the mammalian complex. the question arises therefore if the structural complexity of complex i from higher organisms correlates with its ab ...200314507430
influence of temperature on the immune response of carp to lipopolysaccharide and whole cell aeromonas bestiarum vaccines.immunisation of carp, cyprinus carpio, with four different experimental vaccines for protection against a. bestiarum was tested. carp immunized with different antigens of a. bestiarum p1s, and kept at 18 degrees c responded immunologically by relatively high titers of antibodies. antibody production was inhibited at 12 degrees c.200314509353
immunomodulatory activity of the dimerized lysozyme klp-602 after earlier suppression by atrazine in carp (cyprinus carpio l.).the purpose of this study was to determine the possibility of modulation of the cellular immune response with the dimer of lysozyme klp-602 in fish after suppression induced by atrazine. in the in vivo studies fish were intoxicated with atrazine at a dose of 1/4 ld50, then after 48 hours the dimer of lysozyme klp-602 was administerd at a dose of 0.02 mg/kg of body weight. after 2, 3, 5, 7 and 10 days after intoxication cells from the blood and pronephros of carp were isolated. in the study we de ...200314509361
genomic organization of the rdna cistron of the teleost fish cyprinus carpio.the seasonal adaptation of the teleost cyprinus carpio to the cyclical changes of its habitat demands physiological compensatory responses. the process involves profound nucleolar adjustments and remarkable changes in rrna synthesis, which affects ribosomal biosynthesis. in this context, we have demonstrated that the synthesis of several proteins involved in ribosomal biogenesis as protein kinase ck2, ribosomal protein l41 and nucleolin, as well as u3 snornp, are differentially regulated in summ ...200314513719
reno-, hepato- and splenomegaly of common carp fingerlings (cyprinus carpio l.) diseased in swimbladder inflammation caused by sphaerospora renicola dyková et lom, 1982.the weight of internal organs (swimbladder, kidney, liver, spleen) in relation to the body weight was studied in common carp fingerlings divided into three groups on the basis of swimbladder appearance and microscopic examination of the kidney. the fish had been collected from different hungarian fish farms at the time when swimbladder inflammation (sbi) usually occurs (in july and august). the first group comprised fish with severe signs of sbi and massive renal sphaerosporosis, the second grou ...200314516160
cardiac ankyrin repeat protein is preferentially induced in atrophic myofibers of congenital myopathy and spinal muscular atrophy.cardiac ankyrin repeat protein (carp), which is structurally characterized by the presence of four ankyrin repeat motifs in its central region, is believed to be localized in the nucleus and to participate in the regulation of cardiac-specific gene expression in cardiomyocytes. however, we recently found that carp was induced in skeletal muscle by denervation, leading us to speculate that carp may be induced under some pathological conditions. in the present study, we immunohistochemically analy ...200314516314
asymmetric temporal properties in the receptive field of retinal transient amacrine cells.the speed of signal conduction is a factor determining the temporal properties of individual neurons and neuronal networks. we observed very different conduction velocities within the receptive field of fast-type on-off transient amacrine cells in carp retina cells, which are tightly coupled to each other via gap junctions. the fastest speeds were found in the dorsal area of the receptive fields, on average five times faster than those detected within the ventral area. the asymmetry was similar ...200314517270
hatching of common carp (cyprinus carpio l.) embryos stored at 4 and -2 degrees c in different concentrations of methanol and sucrose.the hatching performance of common carp (cyprinus carpio l.) embryos was examined after 12-72-h storage at 4 and -2 degrees c using different concentrations of sucrose (0.1, 0.25, 0.5 and 1.0 m or 3.42, 8.55, 17.10 and 34.2%), methanol (meoh) (0.5, 1.0, 1.5, 2.0, 2.5, 3.0 and 3.5 m or 1.6, 3.2, 4.8, 6.4, 8.0, 9.6 and 11.2%), or varying concentrations of methanol in 0.5 m (17.10%) sucrose. for sucrose, 0.5 m (17.10%) showed the maximum survival (41+/-1% (12 h) to 11+/-1.5% (72 h)) at 4 degrees c. ...200314519463
the changes in common carp blood after short-term zinc exposure.blood zinc level, hematological parameters and blood cell morphology were evaluated in common carp immediately after 3 h exposure to 20 mg dm(-3) of zinc (zn0), and in 24, 48 and 96 hours after the end of it (zn24, zn48, zn96). blood zinc level in the non-exposed fish was 8 mg dm(-3), reached a maximum of 20 mg dm(-3) in zn48, while it dropped to 9 mg dm(-3) in zn96. zinc caused a stress reaction in fish indicated by an increase in hematocrit value in zn0, and elevated plasma glucose level and t ...200314535641
multiple acute temperature stress affects leucocyte populations and antibody responses in common carp, cyprinus carpio l.stress is a potential factor causing increased susceptibility of fish to pathogens. in this study, stress-induced immunological changes that may contribute to a decreased immune status were investigated. a 3 h drop in ambient water temperature of 9 degrees c was used as a relative mild and acute stress model for carp. effects of this stressor on the dynamics of leucocyte populations were determined with specific monoclonal antibodies. the relative number of circulating b-lymphocytes in the total ...200314550666
molecular cloning and characterization of crucian carp (carassius auratus l.) interferon regulatory factor 7.interferon (ifn) can induce an antiviral state via interferon-regulatory transcription factors (irfs), which bind to and control genes directed by the interferon-stimulated response element (isre). here we describe a fish irf, termed cairf7, cloned from a subtractive cdna library which is constructed with mrnas obtained from crucian carp (carassius auratus l.) blastulae embryonic (cab) cells infected by uv-inactivated gchv and mock-infected cells. cairf7 cdna was found to be 1816 bp in length, w ...200314550671
stimulation of antioxidant enzymes levels in carp (cyprinus carpio l.) infected by ptychobothrium sp. (cestoda).increased antioxidant enzymatic activities were observed in carp parasitised by ptychobothrium sp. when compared with healthy fish. this antioxidant response could contribute to neutralise the oxidative stress normally induced by parasitism.200314550672
development of quantitative vitellogenin-elisas for fish test species used in endocrine disruptor screening.the yolk protein precursor vitellogenin (vtg) in plasma has proved to be a simple and sensitive biomarker for assessing exposure of fish to environmental estrogens. within international bodies such as the organization for economic cooperation and development (oecd) work is ongoing to develop screening and testing programmes for endocrine disrupting effects of new chemicals, and in the focus of this development are the fish test species common carp (cyprinus carpio), fathead minnow (pimephales pr ...200414551668
feminization of wild carp, cyprinus carpio, in a polluted environment: plasma steroid hormones, gonadal morphology and xenobiotic metabolizing system.wild carp, cyprinus carpio, were sampled in january and march 2000 in a section of the anoia river (ne spain) known to be polluted by estrogenic compounds. at each sampling time, three groups were distinguished: (1) apparently normal males; (2) apparently normal females; and (3) affected fish. the latter were characterized by the simultaneous development of male and female tissue in their gonads at a macroscopical level (six out of 31 fish sampled at this particular point), or testicular atrophy ...200314559296
growth retardation and histopathology of common carp (cyprinus carpio) exposed to gallium. 200314560373
temperature-dependent developmental variation in lobster muscle myosin heavy chain isoforms.the temperature- and developmental-regulation of myosin heavy chain (myhc) expression and primary sequence was investigated in the abdominal musculature of developing homarus gammarus larvae acclimated to 10, 14 and 19+/-1 degrees c. myhc loop 1 (atp binding) and loop 2 (actin binding) regions were sequenced and compared. the deduced amino acid sequence of myhc loop 1 showed a development-related increase in net charge from +1 to +2 between larval stages 1 and 2, which was not temperature-depend ...200314563558
cyclic amp-receptor proteins in human salivary glands.in mammalian species, cyclic amp receptor proteins (carp) are the regulatory (r) subunits of cyclic amp-dependent protein kinase (pka), the cellular effector of cyclic amp-mediated signal transduction. an isoform of the pka type ii r subunit (rii), carp, is a polyfunctional protein, present in most tissues and cells. it is expressed in salivary and other glands of rodents, and secreted into the saliva of rats and man. the aim of the present study was to determine the expression of carp in human ...200214566599
[effect of zinc ions, manganese and lead ions on the thermostable proteins of carp liver].effect of comparatively low concentration of the zinc, manganese, lead ions (0.1, 0.13 and 0.01 mg/l respectively) on the thermostable proteins (tp) of the carp liver after fish incubation for 14 days was studied. it is revealed that about 30 of zinc, 40% of manganese and 6% of lead were bound in the tp solution. the results of chromatography on sephadex g-75 show, that the metallothionein-like proteins (mt, m-6 kda) were the primary metal-binding proteins among the tp for zinc and manganese, an ...201514574743
long-term exposure effects in vitellogenin, sex hormones, and biotransformation enzymes in female carp in relation to a sewage treatment works.in the present study, the estrogenic response in the reproductive cycle of female carp, cyprinus carpio, in relation to a sewage treatment works (stws) was measured as: alterations in plasmatic and hepatic vitellogenin (vtg) content, plasma levels of testosterone (t) and estradiol (e2), and effects on key enzymes of phases i and ii biotransformation, ethoxyresorufin o-deethylase (erod) and glutathione s-transferase (gst). low plasma vtg content in females collected 4 km downstream of an effluent ...200314575677
glycinergic input to carp retinal ganglion cells may be mediated by glycine receptors with homologous kinetics.current responses of carp retinal ganglion cells (rgcs) retrogradely labeled and freshly dissociated to rapid application of glycine were recorded by whole-cell patch clamp techniques and effects of glycine antagonists on these responses were analyzed. the current response to maintained application of glycine at a concentration higher than 30 microm exhibited desensitization, which was well fitted to a monoexponential function. strychnine (1 microm), a glycine receptor antagonist, completely blo ...200314575889
the muscle ankyrin repeat proteins: carp, ankrd2/arpp and darp as a family of titin filament-based stress response molecules.carp, ankrd-2/arpp, and darp, are three members of a conserved gene family, referred to here as marps (muscle ankyrin repeat proteins). the expression of marps is induced upon injury and hypertrophy (carp), stretch or denervation (ankrd2/arpp), and during recovery following starvation (darp), suggesting that they are involved in muscle stress response pathways. here, we show that marp family members contain within their ankyrin repeat region a binding site for the myofibrillar elastic protein ti ...200314583192
efficient vaccine against the virus causing a lethal disease in cultured cyprinus carpio.we have isolated a virus, which causes a mortal disease in cultured ornamental koi and common carps (cyprinus carpio) in many countries worldwide. this unclassified virus, which causes nephritis and gill necrosis, and so has been given the name carp nephritis and gill necrosis virus (cngv), has a morphology resembling the herpes virus, but bears a genomic dna of ca 250-300 kbp. so far, both others and we have been unable to find cngv-dna sequences possessing a significant similarity to known dna ...200314585675
binding of perfluorinated fatty acids to serum proteins.perfluorooctane sulfonic acid (pfos) accumulates in the liver and blood of exposed organisms. the potential for these surfactant molecules to interfere with hormone/protein interactions in blood is of concern given the importance of these interactions. the pfos binding to serum proteins was investigated by assessing its ability to displace a variety of steroid hormones from specific binding proteins in the serum of birds and fishes. perfluorooctane sulfonic acid had only a weak ability to displa ...200314587903
regulation of action potential duration under acute heat stress by i(k,atp) and i(k1) in fish cardiac myocytes.the mechanism underlying temperature-dependent shortening of action potential (ap) duration was examined in the fish (carassius carassius l.) heart ventricle. acute temperature change from +5 to +18 degrees c (heat stress) shortened ap duration from 2.8 +/- 0.3 to 1.3 +/- 0.1 s in intact ventricles. in 56% (18 of 32) of enzymatically isolated myocytes, heat stress also induced reversible opening of atp-sensitive k+ channels and increased their single-channel conductance from 37 +/- 12 ps at +8 d ...200414592934
precise and efficient cleavage of recombinant fusion proteins using mammalian aspartic proteases.expression of recombinant proteins as translational fusions is commonly employed to enhance stability, increase solubility and facilitate purification of the desired protein. in general, such fusion proteins must be cleaved to release the mature protein in its native form. the usefulness of the procedure depends on the efficiency and precision of cleavage and its cost per unit activity. we report here the development of a general procedure for precise and highly efficient cleavage of recombinant ...200314600208
the influence of translational selection on codon usage in fishes from the family cyprinidae.in this paper, the main factors shaping codon usage in three species of fishes that belong to the family cyprinidae (namely brachidanio rerio, cyprinus carpio, and carassius auratus) are reported. correspondence analysis (coa), a commonly used multivariate statistical approach, was used to analyze codon usage bias. our results show that the main trend is strongly correlated with the gc(3) content at silent sites of each sequence. on the other hand, the second axis discriminates between presumed ...200314604802
feeding level and individual metabolic rate affect delta 13c and delta 15n values in carp: implications for food web studies.stable isotope analyses are often used to calculate relative contributions of multiple food sources in an animal's diet. one prerequisite for a precise calculation is the determination of the diet-tissue fractionation factor. isotopic ratios in animals are not only affected by the composition of the diet, but also by the amount of food consumed. previous findings regarding the latter point are controversial. as stable isotope analyses have often been used to investigate aquatic food webs, an exp ...200414608500
expression in escherchia coli and purification of sea bass (dicentrarchus labrax) interleukin 1beta, a possible immunoadjuvant in aquaculture.interleukin 1beta (il-1beta) is a pleiotropic cytokine that plays a pivotal role in regulating immune responses. our group has recently cloned il-1beta from sea bass (dicentrarchus labrax), one of the main mediterranean aquacultured fish species. the cdna is 1292 bp and codes for a deduced peptide of 29.4 kda with a pi of 5.1. as for trout and carp il-1beta precursor sequence, no candidate cut site for ice (il-1beta converting enzyme) enzyme was apparent in the alignments of sea bass il-1beta wi ...200414612985
use of vitellogenin mrna as a biomarker for endocrine disruption in feral and cultured fish.the presence of the female-specific yolk protein precursor vitellogenin in blood and liver from male fish is widely used as an indicator of endocrine disruption. we studied the induction of vitellogenin mrna in liver from several species of fish, both maintained in fish tanks or captured in the wild. our procedure requires minute amounts of liver samples (down to 50 mg), and can be applied to field samples if the appropriate rna-stabilisation agent is used. we used reverse-transcriptase pcr and ...200414618290
recovery of fertility in male hybrids of a cross between goldfish and common carp by transplantation of pgc (primordial germ cell)-containing graft.in germ-line chimera, gametes originate from both the donor and recipient. in order to increase the proportion of gametes from the donor, the elimination or reduction of primordial germ cells (pgcs) from the recipient is required. in the present study, histological and genetic analyses were performed in the chimeric fish obtained when sterile goldfish x common carp hybrid and fertile goldfish embryos were used as a recipient and donor, respectively. chimerism was induced by transplantation of th ...200314620952
cloning, characterization and expression analysis of interleukin-10 from the common carp, cyprinus carpio l.interleukin (il)-10 was cloned from the common carp (cyprinus carpio l.) using il-10 primers from carp head kidney following stimulation with concanavalin a and lipopolysaccharide. the cdna consisted of a 1096 bp sequence containing a 55 bp 5' untranslated region and a 498 bp 3' untranslated region. an open reading frame of 543 bp encoded a putative 180 amino acid protein with a putative signal peptide of 22 amino acids. the signature motif of il-10 is conserved in carp sequence. a 2083 bp genom ...200314622251
molecular characterization of d-amino acid oxidase from common carp cyprinus carpio and its induction with exogenous free d-alanine.a full-length cdna encoding d-amino acid oxidase (dao, ec 1.4.3.3) was cloned and sequenced from the hepatopancreas of carp fed a diet supplemented with d-alanine. this clone contained an open reading frame encoding 347 amino acid residues. the deduced amino acid sequence exhibited about 60 and 19-29% identity to mammalian and microbial daos, respectively. the expression of full-length carp dao cdna in escherichia coli resulted in a significant level of protein with dao activity. in carp fed the ...200314622982
common genomic response in different mouse models of beta-adrenergic-induced cardiomyopathy.although beta-adrenergic receptor (ar) blockade therapy is beneficial in the treatment of heart failure, little is known regarding the transcriptional mechanisms underlying this salutary action.200314623810
uncoupling of horizontal cells alters the receptive fields of retinal bipolar cells.effects of uncoupling of horizontal cells by 1-octanol, a non-specific gap junction uncoupling agent, on the receptive field organization of cone-dominant bipolar cells were investigated in isolated, superfused carp retina, using intracellular recording techniques. at 1 mm, 1-octanol increased responses of cone driven horizontal cells to light spots, but decreased those to light annuli, indicating a reduction of the receptive field size of these cells by uncoupling. furthermore, 1-octanol elimin ...200314625439
cadmium-induced cellular and immunological responses in cyprinus carpio infected with the blood parasite, sanguinicola inermis.little is known about immune responses in teleosts as linked to the aetiology of pollutants and parasitic diseases and in particular their combined effects on the host. cadmium(cd)-mediated immunological responses in the thymus and pronephros of juvenile carp (cyprinus carpio), experimentally infected with the blood parasite, sanguinicola inermis (trematoda: sanguinicolidae) for 30 days followed by an exposure to 0.1 mg cd2+ l(-1) for 48 or 168 h were investigated. differential organ-specific ch ...200314627452
complete sequencing and expression of three complement components, c1r, c4 and c1 inhibitor, of the classical activation pathway of the complement system in rainbow trout oncorhynchus mykiss.three complement components, c1r, c4 and c1 inhibitor, of the classical activation pathway have been fully sequenced and their expression investigated in rainbow trout (oncorhynchus mykiss). trout c1r cdna encodes a 707-amino-acid (aa) protein with a theoretical m(r) of 77,200. the trout translation shows highest homology with carp c1r/s, and lower, equal homologies to mammalian c1r and c1s, and masps from other vertebrate species. however, phylogenetic analysis and structural features suggest t ...200314628103
dynamics of microcystins-lr and -rr in the phytoplanktivorous silver carp in a sub-chronic toxicity experiment.a sub-chronic toxicity experiment was conducted to examine tissue distribution and depuration of two microcystins (microcystin-lr and microcystin -rr) in the phytoplanktivorous filter-feeding silver carp during a course of 80 days. two large tanks (a, b) were used, and in tank a, the fish were fed naturally with fresh microcystis viridis cells (collected from a eutrophic pond) throughout the experiment, while in tank b, the food of the fish were m. viridis cells for the first 40 days and then ch ...200414638304
coexistence of a sexual and an unisexual form stabilized by parasites.an unisexual species (either parthenogenetic or gynogenetic form) often coexists sympatrically with a closely related anisogamous sexual species, forming a unisexual complex. this is puzzling because, all else being equal, a sexual population with the two-fold cost of sex (or the cost of producing males) cannot coexist with a unisexual population. this suggests that some ecological processes are at work to make possible the coexistence of the unisexual complex. field and laboratory studies sugge ...200414643188
common carp insulin-like growth factor-i gene: complete nucleotide sequence and functional characterization of the 5'-flanking region.insulin-like growth factor-i (igf-i) plays an important role in the growth and development of fish. to understand the molecular mechanism which controls the transcription of the igf-i gene in common carp, we have cloned and completely sequenced the igf-i gene and the 5'-flanking region from a local tropical fish, the common carp (cyprinus carpio), and characterized its promoter activity by transfection into human embryonic kidney (293ghr) cells which express the human growth hormone (gh) recepto ...200314644506
dynamics of experimental production of thelohanellus hovorkai (myxozoa: myxosporea) in fish and oligochaete alternate hosts.the dynamics of development and production of thelohanellus hovorkai (myxozoa) were examined to investigate factors inducing haemorrhagic thelohanellosis in carp, cyprinus carpio l. fresh actinospores of t. hovorkai were harvested from the oligochaete alternate host, branchiura sowerbyi, and used for infection experiments with myxosporean-free carp. visualization of actinospores by fluorescent labelling revealed that sporoplasms penetrated the gill filaments of carp immersed in an actinospore su ...200314653315
susceptibility of zebrafish (danio rerio) to a model pathogen, spring viremia of carp virus.to improve our understanding of the genetic basis of fish disease, we developed a pathogen model, using zebrafish (danio rerio) and spring virema of carp virus (svcv). replicate groups of 10 fish were acclimated to 20 or 24 degrees c, then were exposed to svcv concentrations of 10(3) to 10(5) plaque-forming units per milliliter (pfu/ml) of water and observed daily. in a second trial, fish were acclimated to 15 degrees c, and replicate groups of 10 fish were exposed to svcv at a concentration of ...200314655994
the thermal contribution to photoactivation in a2 visual pigments studied by temperature effects on spectral properties.effects of temperature on the spectral properties of visual pigments were measured in the physiological range (5-28 degrees c) in photoreceptor cells of bullfrog (rana catesbeiana) and crucian carp (carassius carassius). absorbance spectra recorded by microspectrophotometry (msp) in single cells and sensitivity spectra recorded by electroretinography (erg) across the isolated retina were combined to yield accurate composite spectra from ca. 400 nm to 800 nm. the four photoreceptor types selected ...201414658769
one-step, non-denaturing purification method of carp (cyprinus carpio) vitellogenin.a new single-step purification procedure was developed to purify carp (cyprinus carpio) vitellogenin (vtg), from estradiol-treated carp plasma. this method was performed by high performance liquid weak anion-exchange chromatography, using a discontinuous elution gradient of nacl (0-0.5 m, steps of 12.5 mm/4 min). sds and native-page analysis, of treated-fish plasma and purified solution, showed the appearance of a 370 kda phospholipoprotein, composed of two 130 kda monomers, with all vtg charact ...200414659439
analysis of genes expressed in head kidney of common carp cyprinus carpio l treated with cortisol.we analyzed genes expressed from head kidney of common carp cyprinus carpio l. treated with cortisol. the results of single-pass sequencing of expressed sequence tags (ests) from 188 clones (au240288-au240367, au301120-au301227) from kidney cdna are presented. one-hundred-twenty-seven clones (67.6%) were completely unknown and are likely to represent newly described genes, whereas 61 clones (32.4%) were identified based on matches to sequences in the database. the putative genes contain several ...200314662309
cholesterol content in meat of some poultry and fish species as influenced by live weight and total lipid content.total cholesterol content in 24 fillets (f) of males and females of common carp, 35 samples of male chicken breast meat (bm) and thigh meat (tm), and 48 samples of male turkey bm and tm, respectively, was determined by high-performance liquid chromatography after total lipid (tl) extraction using n-hexane/2-propanol mixture. cholesterol content in male carp fillets (77.6 mg/100 g) was higher (p < 0.001) in comparison with females (69.4 mg/100 g). irrespective of the sex differences, cholesterol ...200314664530
the influence of gabaergic drugs upon lh secretion from the pituitary of the common carp: an in vitro study.in the present study, the perifusions of whole pituitary glands of spermiating male common carp were performed in the presence of several gabaergic drugs. muscimol (agonist of gaba(a) receptors) and bicuculline (the antagonist of the same type of gaba receptors) did not modify basal lh release. lh basal secretion was not modified when pituitaries were perifused with baclofen--an agonist of gabab receptors. on the other hand, baclofen at doses of 10(-8) and 10(-4) m significantly decreased gnrh-a ...200114666167
inflammatory interactions in fish exposed to pollutants and parasites: a role for apoptosis and c reactive protein.although previous studies have highlighted the inflammatory responses of fish infected with parasites and exposed to pollutants, very little is known about how these two stressors interact within the fish. in this review, which also contains original data, the effect of these two parameters on the fish inflammatory response is assessed and, in particular, the role of apoptosis and the acute phase protein, c reactive protein, is evaluated. in cyprinus carpio exposed to 0.5 mg nh4+ l(-1) or 0.1 mg ...200314667174
small indigenous fish species in bangladesh: contribution to vitamin a, calcium and iron intakes.fish play an important role in the bangladeshi diet, constituting the main and often irreplaceable animal source food in poor rural households. fish consumption is dominated by wild small (length <25 cm) indigenous fish species (sis). the vitamin a content in sis varies, from <100 microg of retinol equivalents (re)/100 g raw edible parts, to >2,500 microg re/100 g raw edible parts in mola (amblypharyngodon mola). the study addressed the dietary contribution of fish to vitamin a, calcium and iron ...200314672305
variation of microcystins in a lake for water supply.a site investigation was conducted to correlate the relationships between microcystins (mc) concentration and algal growth in dianchi lake in china. laboratory experiments were undertaken to test the effects of sediment adsorption, photoirradiation and biodegradation on microcystins removal. bioaccumulation of microcystins was also determined using silver carp fish. it was observed that mc concentrations varied in accordance with algae growth in dianchi lake. the results obtained in the laborato ...200314672320
the immunosuppressive effect of alpha-permethrin on indian major carp, rohu (labeo rohita ham.).the immunosuppressive effects of bath exposure to a sub lethal concentration of the synthetic pyrethroid alpha-permethrin (3.05 x 10(-4) mg l(-1)) in the indian major carp, labeo rohita was studied after 45 days' exposure. in some groups, the effects of alpha-permethrin on non-specific defences and serum enzymes of carp were investigated after challenge with aeromonas hydrophila. several nonspecific immune responses and serum enzymes were reduced after exposure of alpha-permethrin. bactericidal ...200414675832
assessment of bioavailable pah, pcb and ocp concentrations in water, using semipermeable membrane devices (spmds), sediments and caged carp.bioavailable water concentrations of polycyclic aromatic hydrocarbons (pahs), polychlorinated biphenyls (pcbs) and organochlorine pesticides (ocps) were assessed at several freshwater sites in and around the city of amsterdam. carp (cyprinus carpio) were caged for 4 weeks at 10 sites, together with semipermeable membrane devices (spmds). in addition, sediment samples were taken at each site. spmds and sediments were analysed for pahs, pcbs and ocps. carp muscle tissues were analysed for pcbs and ...200414675846
[geese, carp, honey cakes, stollen. history, medicine and magic around the christmas feast]. 200314681776
the morpho-anatomy and histology of the pineal complex in a major indian carp, catla catla: identification of the pineal photoreceptor cells and their responsiveness to constant light and constant darkness during different phases of the annual reproductive cycle.in contrast to mammals in which the pineal gland is a discrete structure situated dorsally in the brain, the "pineal gland" in teleost fishes is composed of a number of separate but connected constituent parts, collectively described as the "pineal complex." in this paper, we have described the pineal complex in a common indian carp, catla catla, which exhibits an annual reproductive cycle. attempts have been made to (a) provide an in-depth description of the structure of the pineal complex; and ...200314682472
identification and expression analysis of two ifn-inducible genes in crucian carp (carassius auratus l.).interferon (ifn) exerts its antiviral effects mainly through activation of a subset of ifn-stimulated genes (isg), but relatively few of fish isgs have been isolated and characterized so far. here, we report two fish isgs, termed caifi58 and caifi56, cloned from a subtractive cdna library constructed with mrnas obtained from crucian carp (carassius auratus l.) blastulae embryonic (cab) cells infected by uv-inactivated gchv and mock-infected cells. database search revealed that both isgs had a hi ...200414697509
neurophysiologic detector-a selective and sensitive tool in high-performance liquid chromatography.in the present study neurons from the olfactory system of the fish crucian carp, carassius carassius l. were used as components in an in-line neurophysiologic detector (npd) to measure physiological activities following the separation of substances by high-performance liquid chromatography (hplc). the skin of crucian carp, c. carassius l. contains pheromones that induce an alarm reaction in conspecifics. extra-cellular recordings were made from neurons situated in the posterior part of the media ...200414698234
molecular characterization of cathepsin l from hepatopancreas of the carp cyprinus carpio.purified cathepsin l from carp, cyprinus carpio, consists of a 28 kda single-chain form that is different from the 24 and 5 kda mammalian two-chain form. we cloned cathepsin l from carp hepatopancreas. the sequence consisted of a 1490 bp cdna and a 1014 bp open reading frame, encoding a deduced protein of 337 amino acids that is likely processed to an active enzyme (single-chain form) with 222 amino acids. its similarity to other types of vertebrate cathepsin l is less than 69%. mammalian cathep ...200414698917
purification and characterization of myofibril-bound serine protease from lizard fish (saurida undosquamis) muscle.myofibril-bound serine protease (mbsp) from lizard fish (saurida undosquamis: synodontidae) skeletal muscle was purified to homogeneity with higher purification (1260-fold) and higher recovery (7%) than our previous report in lizard fish (saurida wanieso). the new purification method combines a heat-treatment for dissociation from washed myofibrils, acid-treatment at ph 5.0 before and after lyophilization, and alcohol-treatment, followed by two column chromatographies. the molecular mass of the ...200414698920
purification and cloning of an endogenous protein inhibitor of carp nephrosin, an astacin metalloproteinase.nephrosin is a newly discovered member of the astacin family. it is a secreted proteinase and is present in carp head kidney, kidney, and spleen, all of which are responsible for immune and hematopoietic functions in fish. a complex formed by nephrosin and its inhibitor was purified from carp kidney extract by heparin affinity column chromatography. the presence of the nephrosin-inhibitor complex in different tissues was examined by immunoblotting with polyclonal antisera against the purified ne ...200414709555
establishment and characterization of two new cell lines derived from flounder, paralichthys olivaceus (temminck & schlegel).two new cell cultures from flounder, paralichthys olivaceus (temminck & schlegel), flounder fin (ffn) cells from fin tissue and flounder spleen (fsp) cells from spleen tissue, were established and characterized. the cells multiplied well in eagle's minimum essential medium, supplemented with 10% foetal bovine serum, and have been subcultured more than 100 times, becoming continuous cell lines. modal diploid chromosome number of ffn and fsp cells was 64 and 62, respectively. polymerase chain reac ...200314710758
effects of various hormones on the sexual maturity of european eel (anguilla anguilla l.) females from farm and lakes.previously described and alternative methods of the induction of sexual maturation in the european eel were investigated. weekly administrations of a gonadoliberin agonist (nnrh-a=d-phe6-gnrh-ea) did not induce statistically significant effect on the gonads of treated eels in none of the dosages used (0.1 microg and 10 microg/fish). carp pituitary extract and carp pituitary extract together with a dopamine antagonist caused considerable external changes (increase in eye size) and significant gon ...200314711035
identification and induction of hsp70 gene by heat shock and cadmium exposure in carp.a member of the multi-gene family, encoding 70 kd stress proteins, was identified from the common carp (cyprimus carpio). homologies, observed at both nucleic acid and amino acid levels, and also the intronless structure of this gene, strongly suggest that it corresponds to a heat-inducible hsp70 gene in carp. gene-specific primers were selected and used in rt-pcr reactions to measure the basal hsp70 mrna levels and to follow the inducer-specific expression of this gene in different tissues duri ...200314711036
computational tools for modeling electrical activity in cardiac tissue.computer models offer many attractive benefits. however, the modeling of cardiac tissue is computationally expensive due to several physical constraints which result in fine spatiotemporal discretization over large spatiotemporal regions. our laboratory has been actively trying to develop new techniques to make large scale cardiac simulations tractable over the past 15 years. this paper describes the latest modeling software that our group has developed, called carp (cardiac arrhythmias research ...200314716595
comet assay and micronucleus test in circulating erythrocytes of cyprinus carpio specimens exposed in situ to lake waters treated with disinfectants for potabilization.the detection of a possible genotoxic effect of surface water treated with disinfectants for potabilization is the aim of the present work. the comet assay and the micronucleus test were applied in circulating erythrocytes of cyprinus carpio. young specimens (20-30 g) were exposed in experimental basins, built within the potabilization plant of castiglione del lago (perugia, italy). in this plant the water of the trasimeno lake is treated and disinfected for potabilization before it is distribut ...200414729366
isolation of a rhabdovirus during outbreaks of disease in cyprinid fish species at fishery sites in england.a virus was isolated during disease outbreaks in bream abramis brama, tench tinca tinca, roach rutilis rutilis and crucian carp carassius carassius populations at 6 fishery sites in england in 1999. mortalities at the sites were primarily among recently introduced fish and the predominant fish species affected was bream. the bream stocked at 5 of the 6 english fishery sites were found to have originated from the river bann, northern ireland. most fish presented few consistent external signs of d ...200314735920
minor effect of depletion of resident macrophages from peritoneal cavity on resistance of common carp cyprinus carpio to blood flagellates.carp cyprinus carpio macrophages were depleted by intraperitoneal (i.p.) injection of clodronate-liposomes for the in vivo study of the effect of macrophage depletion on the resistance of carp to infection with blood flagellate parasites. clodronate released inside the cell induces apoptosis of (murine) macrophages. following i.p. injection of carp with liposomes alone, but not with trypanoplasma borreli, neutrophilic granulocytes rapidly migrated from the head kidney to the peritoneal cavity. t ...200314735923
effect of epidermal papillomatosis on survival of the freshwater fish rutilus rutilus.epidermal papillomatosis occurs in several marine and freshwater fish species. previously, papillomatosis has been shown to induce mortality in juvenile carp. we studied the effect of epidermal papillomatosis on the survival of adult male roach rutilus rutilus by caging naturally diseased, marked (by us) fish in the field. within the constraints of the experimental design, there was no difference in survival between healthy, slightly diseased and heavily diseased fish. therefore, we conclude tha ...200314735936
debromination of the flame retardant decabromodiphenyl ether by juvenile carp (cyprinus carpio) following dietary exposure.the congener 2,2',3,3',4,4',5,5',6,6'-decabromodiphenyl ether (bde 209) is the primary component in a commonly used flame retardant known as decabde. this flame retardant constitutes approximately 80% of the world market demand for polybrominated diphenyl ethers (pbdes). because this compound is very hydrophobic (log k(ow) approximately 10), it has been suggested that bde 209 has very low bioavailability, although debromination to more bioavailable metabolites has also been suggested to occur in ...200414740725
induction and myofibrillar targeting of carp, and suppression of the nkx2.5 pathway in the mdm mouse with impaired titin-based signaling.muscular dystrophy with myositis (mdm) is a recessive mouse mutation that is caused by a small deletion in the giant elastic muscle protein titin. homozygous mdm/mdm mice develop a progressive muscular dystrophy, leading to death at approximately 2 months of age. we surveyed the transcriptomes of skeletal muscles from 24 day old homozygous mdm/mdm and +/+ wild-type mice, an age when mdm animals have normal passive and active tensions and sarcomeric structure. of the 12488 genes surveyed (u74 aff ...200414741210
pluripotency of cryopreserved blastomeres of the goldfish.to examine the pluripotency of cryopreserved blastomeres, we transplanted them into blastula. donor blastomeres were prepared from blastula of goldfish (carassius auratus) and cryopreserved in liquid nitrogen for two months. fifty-five percent and 44% of blastomeres survived after thawing. cryopreserved blastomeres were transplanted to the blastula of triploid crucian carp (c. a. longsdorfii), which reproduces gynogenetically in nature. at four days after the operation, resultant chimeric embryo ...200414743512
common carp metallothionein-1 gene: cdna cloning, gene structure and expression studies.metallothionein-1 (mt-1) cdna clones were isolated from a common carp (cyprinus carpio) uninduced hepatopancreas cdna library. northern blot assay using the common carp (cc) mt-1 cdna as a probe showed high fold induction of ccmt mrna levels in the intestine and kidney following exposure to cd2+ and zn2+. using polymerase chain reaction (pcr), primers designed from the cdna sequences allowed the isolation of ccmt-1 gene fragments including the 5'-flanking region. the 600 bp 5'-flanking region of ...200414746911
isolation and characterization of a rhabdovirus from starry flounder (platichthys stellatus) collected from the northern portion of puget sound, washington, usa.the initial characterization of a rhabdovirus isolated from a single, asymptomatic starry flounder (platichthys stellatus) collected during a viral survey of marine fishes from the northern portion of puget sound, washington, usa, is reported. virions were bullet-shaped and approximately 100 nm long and 50 nm wide, contained a lipid envelope, remained stable for at least 14 days at temperatures ranging from -80 to 5 degrees c and grew optimally at 15 degrees c in cultures of epithelioma papulosu ...200414769907
[effect of thiourea on the glucide metabolism of the carp (cyprinus carpio l.)]. 195014792811
[presence of heparin in scales of cyprinus carpio]. 195014822288
[the effect of horse serum injections on the heparinocytes of carp scale]. 195014822357
[control of carp rot in the 6 year plan]. 195114842718
[fluorescyanine (ichthyopterin) obtained from eggs of bombyx mori and carp scales]. 195114859632
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