Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| association of a novel high molecular weight, serine-rich protein (srpa) with fibril-mediated adhesion of the oral biofilm bacterium streptococcus cristatus. | the surface of the oral plaque bacterium streptococcus cristatus is decorated with a lateral tuft of fibrils. the fibrillar tuft functions in the adhesion of s. cristatus to heterologous bacterial species in the plaque biofilm. the tuft typically consists of a densely packed fringe of shorter fibrils 238 +/- 19 nm long with longer, less abundant fibrils 403 +/- 66 nm long projecting through the fringe of short fibrils. the two types of fibrils in the tufts of s. cristatus have been refractory to ... | 2005 | 15836513 |
| effect of khat chewing on 14 selected periodontal bacteria in sub- and supragingival plaque of a young male population. | the habit of chewing khat (catha edulis) for its amphetamine-like effects is highly prevalent in yemen and east africa, and has expanded to western countries. the purpose of this study was to estimate and compare the prevalence and levels of 14 periodontal bacteria in gingival plaque of khat chewers and khat nonchewers, as well as of khat chewing sides and khat nonchewing sides. | 2005 | 15836514 |
| detection of heat shock proteins but not superantigen by isolated oral bacteria from patients with behcet's disease. | we isolated bacteria from periodontal sites and mixed saliva in eight patients with behcet's disease and surveyed them to determine whether they produced heat shock proteins (hsps) and superantigen. cultivable bacterial compositions from periodontal sites and saliva were examined by anaerobic culture using blood agar plates. gram-negative anaerobic rods such as prevotella intermedia, fusobacterium nucleatum, and capnocytophaga species were predominant in the isolates from the subgingival plaque ... | 2005 | 15836518 |
| treponema medium glycoconjugate inhibits activation of human gingival fibroblasts stimulated with phenol-water extracts of periodontopathic bacteria. | oral treponemes are well-known as causative agents of periodontal diseases; however, the details have not been fully clarified. here, we examined the effects of treponema medium glycoconjugate on the activation of human gingival fibroblasts using phenol-water extracts from porphyromonas gingivalis, prevotella intermedia, fusobacterium nucleatum subsp. nucleatum, and actinobacillus actinomycetemcomitans. the phenol-water extracts activated human gingival fibroblasts to mediate il-8 production, as ... | 2005 | 15840783 |
| oral microbiology and genomics. | 2005 | 15853937 | |
| failed dental implants - clinical, radiological and bacteriological findings in 17 patients. | the aim of this study was to evaluate the reasons for implant failure in two oral and maxillofacial units in helsinki. seventeen patients, who lost 30 implants were included in this study. | 2005 | 15878525 |
| tobacco smoking and periodontal microflora in a saudi arabian population. | to explore the subgingival periodontal microflora in a saudi arabian population with a special focus on its relationship with various smoking habits. | 2005 | 15882210 |
| susceptibilities of periodontopathogenic and cariogenic bacteria to antibacterial peptides, {beta}-defensins and ll37, produced by human epithelial cells. | antimicrobial peptides are one of the factors involved in innate immunity. the susceptibility of periodontopathogenic and cariogenic bacteria to the major antimicrobial peptides produced by epithelia was investigated. | 2005 | 15886266 |
| microbial changes in conjunctival flora with 30-day continuous-wear silicone hydrogel contact lenses. | to determine the effect of 30-day continuous-wear silicone hydrogel contact lenses on the conjunctival flora in asymptomatic wearers. | 2005 | 15894879 |
| identification of cultivable microorganisms from primary endodontic infections with exposed and unexposed pulp space. | this study was aimed at comparing the cultivable microorganisms in canals with periapical radiolucencies with exposed and unexposed pulp space. microbiological samples were taken and analyzed from 45 canals with exposed pulp space, and 43 canals with unexposed pulp space. the canal contents were analyzed by aerobic/anaerobic culture, and conventional identification techniques. there were 211 isolates of bacteria belonging to 28 genera and 55 species recovered from exposed canals. in the unexpose ... | 2005 | 15917680 |
| molecular identification of an invasive gingival bacterial community. | a woman with neutropenia developed gingival hyperplasia. biopsy showed invasion of gingival tissue with mats of filamentous organisms, and molecular analysis by polymerase chain reaction and fluorescence in situ hybridization revealed capnocytophaga sputigena, leptotrichia species, and fusobacterium nucleatum. oral bacterial flora may cause invasive gingival disease with hyperplasia in immunocompromised patients. | 2005 | 15937752 |
| antimicrobial susceptibility variation of 50 anaerobic periopathogens in aggressive periodontitis: an interindividual variability study. | the frequent use of antibiotics in developed countries has led to the emergence of widespread bacterial resistance. in this study, the interindividual variability of the antibiotic susceptibility of 50 putative microorganisms in aggressive periodontitis patients has been evaluated by means of vc (variation coefficient). | 2005 | 15943770 |
| microarrays complement culture methods for identification of bacteria in endodontic infections. | the aim of this study was to investigate the microbial composition of necrotic root canals using culture methods and microarray technology. twenty uniradicular teeth with radiographic evidence of periapical bone loss and with no previous endodontic treatment were selected for this study. for molecular diagnosis a dna chip with 20 different species-specific, 16s-rdna-directed catcher probes was used. the microorganisms most frequently detected by the dna chip were: micromonas micros, fusobacteriu ... | 2005 | 15943771 |
| fiber optic fluorescence microprobe for endodontic diagnosis. | successful endodontic therapy requires total debridement as well as complete obturation of the root canal to the cemento-dentinal junction. the goal of this study was to investigate the feasibility of using quantitative fluorescence spectroscopy for the detection and localization of pathological dentin, pulpal remnants, and microorganisms within the root canal. specific aims were to identify: 1) characteristic excitation/emission spectra for healthy dentin, decayed dentin, enamel, and pulp; 2) t ... | 2005 | 15947209 |
| multi-target antimicrobial actions of zinc against oral anaerobes. | zinc is used in oral care products as an antiplaque/antigingivitis agent. our objective was to assess the antimicrobial actions of zinc against oral anaerobes associated with gingivitis, specifically fusobacterium nucleatum and prevotella intermedia, with focus on catabolism and oxidative metabolism. | 2005 | 15958206 |
| mechanism of visible light phototoxicity on porphyromonas gingivalis and fusobacterium nucleatum. | phototoxicity of visible light laser on the porphyrin-producing bacteria, porphyromonas gingivalis, in the absence of photosensitizers and under aerobic conditions was shown in previous studies. recently, we found that the noncoherent visible light sources at wavelengths of 400-500 nm, commonly used in restorative dentistry, induced a phototoxic effect on p. gingivalis, as well as on fusobacterium nucleatum, and to a lesser extent on the streptococci sp. to elucidate the mechanism of this photot ... | 2005 | 15960594 |
| comparison of culture and real-time pcr for detection and quantification of five putative periodontopathogenic bacteria in subgingival plaque samples. | bacterial cultivation is a well-established method for analyzing plaque samples. real-time polymerase chain reaction (pcr) is a novel rapid method for the identification and quantification of periodontopathogenic bacteria that has been recently introduced. in this study, we compared real-time pcr with conventional anaerobic cultivation. | 2005 | 15966886 |
| prevalence of periodontopathic bacteria in aggressive periodontitis patients in a chilean population. | actinobacillus actinomycetemcomitans is considered a major etiologic agent of aggressive periodontitis (agp). other periodontopathic bacteria such as porphyromonas gingivalis are also suspected of participating in aggressive periodontitis although the evidence to support this is controversial. the aim of the present study was to determine the prevalence of eight periodontopathic bacteria in chilean patients with agp. | 2005 | 15974855 |
| fluorescence based measurements of fusobacterium nucleatum coaggregation and of fusobacterial attachment to mammalian cells. | fusobacterium nucleatum is a common oral anaerobe associated with gingivitis, periodontal disease and preterm deliveries. coaggregation among oral bacteria is considered to be a significant factor in dental plaque development. adhesion to host cells was suggested to be important for the f. nucleatum virulence associated with oral inflammation and with preterm births. an uncharacterized fusobacterial galactose inhibitible adhesin mediates coaggregation of f. nucleatum 12230 and f. nucleatum pk159 ... | 2005 | 15993010 |
| protein secretion systems in fusobacterium nucleatum: genomic identification of type 4 piliation and complete type v pathways brings new insight into mechanisms of pathogenesis. | recent genomic analyses of the two sequenced strains f. nucleatum subsp. nucleatum atcc 25586 and f. nucleatum subsp. vincentii atcc 49256 suggested that the major protein secretion systems were absent. however, such a paucity of protein secretion systems is incongruous with f. nucleatum pathogenesis. moreover, the presence of one or more such systems has been described for every other gram-negative organism sequenced to date. in this investigation, the question of protein secretion in f. nuclea ... | 2005 | 15993836 |
| an antibacterial surface on dental implants, based on the photocatalytic bactericidal effect. | it is well known that the moderately roughened surfaces of dental implants enhance direct bone-implant contact. however, rough implant surfaces, as compared to smooth surfaces, are thought to pose a higher risk of bacterial infection when exposed to the oral cavity. | 2005 | 15996357 |
| antimicrobial profiles of periodontal pathogens isolated from periodontitis patients in the netherlands and spain. | antimicrobial resistance of periodontal pathogens towards currently used antibiotics in periodontics has been investigated in a previous study. microbial resistance in the periodontal microflora was more frequently observed in spanish patients in comparison with dutch patients. the aim of the present study was to compare antimicrobial susceptibility profiles of five periodontal bacteria isolated from periodontitis patients in spain and in the netherlands. | 2005 | 15998275 |
| efficacy of photodynamic therapy on inflammatory signs and two selected periodontopathogenic species in a beagle dog model. | current research aims to find alternatives to conventional methods for suppressing periodontopathogenic bacteria. photodynamic therapy (pdt) could be a suitable treatment procedure of periodontal infections. | 2005 | 16018752 |
| identification and characterization of a novel adhesin unique to oral fusobacteria. | fusobacterium nucleatum is a gram-negative anaerobe that is prevalent in periodontal disease and infections of different parts of the body. the organism has remarkable adherence properties, binding to partners ranging from eukaryotic and prokaryotic cells to extracellular macromolecules. understanding its adherence is important for understanding the pathogenesis of f. nucleatum. in this study, a novel adhesin, fada (fusobacterium adhesin a), was demonstrated to bind to the surface proteins of th ... | 2005 | 16030227 |
| vertebral osteomyelitis due to fusobacterium species: report of three cases and review of the literature. | we describe three cases of fusobacterium spp. diskitis and review with attention to risk factors, clinical features, diagnosis, treatment and outcome. in most of the reported cases, a ear-nose-throat infection was found. clinical manifestations were similar to those of classic bacterial vertebral osteomyelitis. clindamycin is the most appropriate antibiotic. the outcome seems to be very good without relapse with appropriate treatment compared to pyogenic vertebral osteomyelitis. | 2005 | 16038751 |
| fusobacterium nucleatum apoptosis-inducing outer membrane protein. | the periodontal pathogen fusobacterium nucleatum induces apoptosis in lymphocytes. we previously identified the autotransporter protein fap2 in f. nucleatum strain pk1594 that induced apoptosis in lymphocytes when expressed in escherichia coli. in this study, we identified protein homologs of fap2 in the transformable f. nucleatum strain atcc 23726, to determine their role in the induction of apoptosis in lymphocytes. we used a new gene-inactivation vector conferring thiamphenicol resistance (ph ... | 2005 | 16040725 |
| [efficacy variation of erythromycin and spiramycin on periopathogens in aggressive periodontitis. an in vitro comparative study]. | erythromycin (ery) and spiramycin (spi) are the most frequently prescribed macrolides by dentists. however, the emergence of resistant anaerobic subgingival bacteria imposes an increased vigilance. this study aims to compare these macrolides efficacy on principal periopathogens. | 2005 | 16087299 |
| the effects of intra-amniotic injection of periodontopathic lipopolysaccharides in sheep. | periodontal disease may cause several complications of pregnancy, including fetal death. the purpose of this study was to investigate in sheep the effects of the intra-amniotic injection of lipopolysaccharide from 3 periodontopathic organisms and to compare these effects with those resulting from similar injection of escherichia coli lipopolysaccharide. the outcomes that were studied included the rates of fetal death and the features of inflammation and lung maturation in survivors. | 2005 | 16098849 |
| differential induction of human beta-defensin expression by periodontal commensals and pathogens in periodontal pocket epithelial cells. | to investigate the possible role of beta-defensins in gingival health and periodontal disease, we examined the effect of several stimuli on the expression of interleukin-8 (il-8), human beta-defensin-1, -2, -3, and -4 (hbd) in primary human diseased gingival epithelial (hge) cell cultures from periodontitis patients by quantitative taqman reverse transcription polymerase chain reaction (rt-pcr). | 2005 | 16101361 |
| cross-reactivity of groel antibodies with human heat shock protein 60 and quantification of pathogens in atherosclerosis. | chronic infections such as those caused by chlamydia pneumoniae and periodontopathic bacteria such as porphyromonas gingivalis have been associated with atherosclerosis, possibly due to cross-reactivity of the immune response to bacterial groel with human heat shock protein (hhsp) 60. | 2005 | 16101965 |
| efficient gene transfer and targeted mutagenesis in fusobacterium nucleatum. | fusobacterium nucleatum is a gram-negative anaerobe important in dental biofilm ecology and infectious diseases with significant societal impact. the lack of efficient genetic systems has hampered molecular analyses in this microorganism. we previously reported construction of a shuttle plasmid, phs17, using the native fusobacterial plasmid pfn1 and an erythromycin resistance cassette. however, the host range of phs17 was restricted to f. nucleatum, atcc 10953, and the transformation efficiency ... | 2006 | 16115683 |
| enhanced pathogenicity of fusobacterium nucleatum adapted to oxidative stress. | fusobacterium nucleatum is an obligate anaerobic bacterium found in the indigenous human microbiota but also recovered from several anaerobic infections. considering the biological and medical relevance of f. nucleatum, the characterization of its response to oxidative stress is needed in order to understand how this anaerobic bacterium survives during an invasive process of oxygenated tissues. influence of oxidative stress by atmospheric oxygen exposure on cellular morphology and pathogenicity ... | 2005 | 16125361 |
| antibacterial activity of aqueous extracts of selected chewing sticks. | this aim of this study was to determine the antibacterial activity in extracts obtained from various nigerian chewing sticks. aqueous extracts from seventeen chewing sticks and the fruit of c. ferruginea, one fruit used in oral hygiene in nigeria, were screened for antibacterial activity against type cultures of staphylococcus aureus, bacillus subtilis, escherichia coli, and pseudomonas aeruginosa. eleven of the test extracts showed activity against at least two of these referenced organisms. mi ... | 2005 | 16127476 |
| development of strain-specific pcr primers based on a dna probe fu12 for the identification of fusobacterium nucleatum subsp. nucleatum atcc 25586t. | the objective of this study was to assess the strain-specificity of a dna probe, fu12, for fusobacterium nucleatum subsp. nucleatum atcc 25586t (f. nucleatum atcc 25586t), and to develop sets of strain-specific polymerase chain reaction (pcr) primers. strain-specificity was tested against 16 strains of f. nucleatum and 3 strains of distinct fusobacterium species. southern blot hybridization revealed that the fu12 reacted exclusively with the hindiii-digested genomic dna of f. nucleatum atcc 2558 ... | 2005 | 16145547 |
| in vitro method to assess the antimicrobial activity and potential efficacy of novel types of wound dressings. | to develop a simple, reproducible in vitro static diffusion method using cellulose disks and defined species to test antimicrobial efficacy of wound dressings. | 2005 | 16162241 |
| microflora in oral ecosystems in subjects with hyposalivation due to medicines or of unknown origin. | to analyse the microbial flora in specific sites in 20 dentate (> or = 16 teeth) subjects with hyposalivation due to medicines or of unknown origin and in 20 controls matched according to age, sex and number of teeth. | 2005 | 16173383 |
| increased antibacterial activity of zinc polycarboxylate cement by the addition of chlorhexidine gluconate in fixed prosthodontics. | this study evaluated the antibacterial activity of water-activated zinc polycarboxylate cement with adjunctive 0.12% chlorhexidine gluconate on the subgingival microbiota in fixed partial dentures. | 2005 | 16220801 |
| antibacterial action of photoactivated disinfection {pad} used on endodontic bacteria in planktonic suspension and in artificial and human root canals. | to measure antibacterial action of photoactivated disinfection (pad) on endodontic bacteria in planktonic suspension and root canals. methods: four bacteria, fusobacterium nucleatum,peptostreptococcus micros, prevotella intermedia and streptococcus intermedius, were tested in suspension. after mixing equal volumes of tolonium chloride and bacterial suspension for 60s, each 200 microl of concentration (>10(6)cfu ml(-1)) was irradiated with light at 633+/-2 nm. each energy dose/tolonium chloride c ... | 2006 | 16239058 |
| universal dna primers amplify bacterial dna from human fetal membranes and link fusobacterium nucleatum with prolonged preterm membrane rupture. | a large number of bacterial species have been identified in fetal membranes after preterm labour (ptl) associated with intrauterine infection by microbiological culture. in this study, we have investigated a molecular and bioinformatic approach to organism identification which surmounts the need for specific and diverse microbiological culture conditions required by conventional methods. samples of fetal membranes were taken from 37 preterm infants, and 6 normal term controls delivered by caesar ... | 2005 | 16254004 |
| effect of sodium alendronate on alveolar bone resorption in experimental periodontitis in rats. | bisphosphonates are potent inhibitors of bone resorption and were shown to inhibit bone resorption in experimental periodontitis by unknown mechanisms. we studied the effect of the aminobisphosphonate sodium alendronate (sa) in experimental periodontitis. wistar rats were subjected to ligature placement around the second upper left molars. | 2005 | 16274309 |
| coaggregation ability of weissella cibaria isolates with fusobacterium nucleatum and their adhesiveness to epithelial cells. | in the present study, we evaluated the ability of weissella cibaria isolated from the oral cavity to coaggregate with fusobacterium nucleatum, and the adhesiveness of these strains to epithelial cells. w. cibaria efficiently coaggregated with f. nucleatum, and adhered to epithelial cells. we tested the effects of various factors on the coaggregation. the coaggregation and adhesiveness of w. cibaria disappeared upon exposure to pronase or licl, suggesting that proteinaceous components on the surf ... | 2005 | 16288835 |
| streptococci dominate the diverse flora within buccal cells. | previously, we reported that intracellular actinobacillus actinomycetemcomitans, porphyromonas gingivalis, and tannerella forsythensis were present within buccal epithelial cells from human subjects, as lesser components of a polymicrobial flora. in this study, we further characterized that intracellular flora by using the same double-labeling techniques to identify fusobacterium nucleatum, prevotella intermedia, oral campylobacter species, eikenella corrodens, treponema denticola, gemella haemo ... | 2005 | 16304448 |
| the major outer membrane protein of fusobacterium nucleatum (foma) folds and inserts into lipid bilayers via parallel folding pathways. | membrane protein insertion and folding was studied for the major outer membrane protein of fusobacterium nucleatum (foma), which is a voltage-dependent general diffusion porin. the transmembrane domain of foma forms a beta-barrel that is predicted to consist of 14 beta-strands. here, unfolded foma is shown to insert and fold spontaneously and quantitatively into phospholipid bilayers upon dilution of the denaturant urea, which was shown previously only for outer membrane protein a (ompa) of esch ... | 2006 | 16310217 |
| identification and localization of extraradicular biofilm-forming bacteria associated with refractory endodontic pathogens. | bacterial biofilms have been found to develop on root surfaces outside the apical foramen and be associated with refractory periapical periodontitis. however, it is unknown which bacterial species form extraradicular biofilms. the present study aimed to investigate the identity and localization of bacteria in human extraradicular biofilms. twenty extraradicular biofilms, used to identify bacteria using a pcr-based 16s rrna gene assay, and seven root-tips, used to observe immunohistochemical loca ... | 2005 | 16332869 |
| effect of mineral trioxide aggregate on cytokine production by peritoneal macrophages. | to test the effect of two commercial brands of grey mineral trioxide aggregate (proroot and mta-angelus) on cytokine production by m1 and m2 inflammatory macrophages. | 2005 | 16343117 |
| the ability of selected oral microorganisms to emit red fluorescence. | some novel caries detection and excavation devices rely on the ability of bacteria to produce red fluorescing compounds. the aim of this study was to examine the ability of selected oral microorganisms to emit red fluorescence. streptococcus mutans, s. oralis, s. salivarius, s. sobrinus, lactobacillus fermentans, l. casei, l. rhamnosus, actinomyces naeslundi, a. israelii, prevotella intermedia, and fusobacterium nucleatum were inoculated onto columbia agar with haemin and vitamin k and incubated ... | 2006 | 16352873 |
| pyogenic liver abscess after colonoscopy in a patient with ulcerative colitis. | 2005 | 16361038 | |
| immediate effect of instrumentation on the subgingival microflora in deep inflamed pockets under strict plaque control. | to investigate (1) reduction in the number of microorganisms obtained directly after subgingival instrumentation, (2) rate of bacterial re-colonization during 2 weeks, under supragingival plaque-free conditions. | 2006 | 16367855 |
| fusobacterium nucleatum transports noninvasive streptococcus cristatus into human epithelial cells. | analysis of human buccal epithelial cells frequently reveals an intracellular polymicrobial consortium of bacteria. although several oral bacteria have been demonstrated to invade cultured epithelial cells, several others appear unable to internalize. we hypothesized that normally noninvasive bacteria may gain entry into epithelial cells via adhesion to invasive bacteria. fusobacterium nucleatum is capable of binding to and invading oral epithelial cells. by contrast, streptococcus cristatus bin ... | 2006 | 16369022 |
| porphyromonas gingivalis heat shock protein vaccine reduces the alveolar bone loss induced by multiple periodontopathogenic bacteria. | heat shock protein (hsp) can be utilized as a vaccine to cross-protect against multiple pathogenic species. the present study was performed to evaluate porphyromonas gingivalis heat shock protein 60 (hsp60) as a vaccine candidate to inhibit multiple bacteria-induced alveolar bone loss. | 2006 | 16409250 |
| antimicrobial efficacy of medicated root canal filling materials. | bacteria remaining within the root canal system are a significant factor in endodontic failures. the aim of this in vitro study was to evaluate the antimicrobial efficacy of commercially available gutta-percha formulations against common endodontic pathogens. standard, tetracycline, or iodoform (also called medicated gutta-percha; mgp) containing gutta-percha were tested against actinomyces israelii, a. naeslundii, enterococcus faecalis, and fusobacterium nucleatum using the agar diffusion metho ... | 2006 | 16427466 |
| genotypic and phenotypic characterization of fusobacteria from chinese and european patients with inflammatory periodontal diseases. | phylogenetic and antigenic studies were performed on 48 human oral fusobacterium strains from chinese patients with either necrotizing ulcerative gingivitis (nug) or gingivitis and on 23 fusobacterium nucleatum or fusobacterium periodonticum strains from european periodontitis patients. alignment of partial 16s rrna gene sequences resulted in a phylogenetic tree that corresponded well with the current classification of oral fusobacteria into f. periodonticum and several subspecies of f. nucleatu ... | 2006 | 16464693 |
| molecular analysis of bacteria in asymptomatic and symptomatic endodontic infections. | the purpose of the present study was to use terminal restriction fragment length polymorphism analysis and the 16s rrna gene clone library to investigate the diversity of the microbiota associated with asymptomatic and symptomatic endodontic infections and to compare the bacterial community structure in these two clinical conditions. samples were taken from asymptomatic endodontic infections associated with chronic periradicular lesions and from symptomatic infections clinically diagnosed as acu ... | 2006 | 16476021 |
| a sonographic short cervix as the only clinical manifestation of intra-amniotic infection. | a sonographically short cervix is a powerful predictor of spontaneous preterm delivery. however, the etiology and optimal management of a patient with a short cervix in the mid-trimester of pregnancy remain uncertain. microbial invasion of the amniotic cavity (miac) and intra-amniotic inflammation are frequently present in patients with spontaneous preterm labor or acute cervical insufficiency. this study was conducted to determine the rate of miac and intra-amniotic inflammation in patients wit ... | 2006 | 16489881 |
| inhibitory effect of weissella cibaria isolates on the production of volatile sulphur compounds. | the objective of this study was to characterize the inhibitory effects of weissella cibaria isolates on volatile sulphur compounds (vsc) production both in vitro and in vivo. | 2006 | 16489950 |
| the efficacy of povidone-iodine products against periodontopathic bacteria. | a total of 8 strains of 6 bacterial species, porphyromonas gingivalis atcc33277 and tdc286, actinobacillus actinomycetemcomitans atcc29523 and jp2, fusobacterium nucleatum no. 2, tannerella forsythensis atcc43937, prevotella intermedia atcc25611 and streptococcus anginosus atcc33397, were treated with povidone-iodine (pvp-i) gargle (pvp-i: 0.47 and 0.23% w/v) or chlorhexidine gluconate (chg) gargle (chg: 0.002% w/v) for 15, 30 or 60 s, after which they were inoculated into various media, culture ... | 2006 | 16490986 |
| anti-inflammatory activity of a high-molecular-weight cranberry fraction on macrophages stimulated by lipopolysaccharides from periodontopathogens. | periodontitis is a chronic inflammatory disease affecting oral tissues. the continuous, high production of cytokines by host cells triggered by periodontopathogens is thought to be responsible for the destruction of tooth-supporting tissues. macrophages play a critical role in this host inflammatory response to periodontopathogens. the aim of this study was to investigate the effect of non-dialyzable material prepared from cranberry juice concentrate on the pro-inflammatory cytokine response of ... | 2006 | 16498070 |
| coaggregation of porphyromonas gingivalis and fusobacterium nucleatum pk 1594 is mediated by capsular polysaccharide and lipopolysaccharide. | previous reports have shown that coaggregation between porphyromonas gingivalis and fusobacterium nucleatum, two important periodontopathogens, is mediated by a galactoside on the surface of p. gingivalis and a lectin on f. nucleatum. in the present study, purified capsular polysaccharide (cps) and lipopolysaccharide (lps) of p. gingivalis pk 1924 (serotype k5) were found to be able to bind to f. nucleatum cells and to inhibit binding of f. nucleatum to p. gingivalis serotype k5. sugar binding s ... | 2006 | 16499621 |
| pyogenic infections of the central nervous system secondary to dental affections--a report of six cases. | pyogenic infections of the central nervous system of dental origin are quite uncommon in industrialized countries. we report six cases with intracerebral (n = 4) and intraspinal (n = 2) infections treated in our hospital. the microbial pathogen was successfully isolated in all patients. fusobacterium nucleatum as well as streptococcus species were found in three cases. bacillus species were identified in two patients. actinomyces was the etiologic agent in one case. all patients suffered from de ... | 2006 | 16501929 |
| the microbiological origin of fluorescence observed in plaque on dentures during qlf analysis. | the aim of this study was to determine the microbiological origin of plaque fluorescence observed during quantitative light-induced fluorescence (qlf) analysis. plaque was sampled from dentures, because of easy accessibility and the homogeneous background provided by the denture tooth during imaging, and the acknowledged comparability to occlusal plaque. forty removable poly(methyl methacrylate) dentures were screened for the presence of fluorescent plaque deposits during qlf analysis. dentures ... | 2006 | 16508267 |
| effects of streptococcus mutans gtfc deficiency on mixed oral biofilms in vitro. | the aim of this study was to examine the influence of glucosyltransferase-gene-negative (gtf-) streptococcus mutans strains unable to synthesize water-insoluble or soluble glucan on the structure and macromolecular diffusion properties of in vitro grown mixed oral biofilms. biofilms modeling supragingival plaque consisted of actinomyces naeslundii omz 745, candida albicans omz 110, fusobacterium nucleatum kp-f2, streptococcus oralis sk 248, veillonella dispar atcc 17748t and one of the s. mutans ... | 2006 | 16508276 |
| [lemierre syndrome variant: hepatic abscesses and hepatic venous thrombosis due to fusobacterium nucleatum septicemia]. | like fusobacterium necrophorum, fusobacterium nucleatum is capable causing lemierre's syndrome. various locations of venous thrombosis have been described associated with fusobacterium sp. septicemia. | 2006 | 16516355 |
| analysis of the antibacterial activity and plaque control benefit of colgate total dentifrice via clinical evaluation and real-time polymerase chain reaction. | this study analyzed, from a combined clinical and molecular biologic perspective, the antibacterial and antiplaque efficacy of colgate total dentifrice (ctd). | 2005 | 16583596 |
| inhibitory effects of macrocarpals on the biological activity of porphyromonas gingivalis and other periodontopathic bacteria. | macrocarpals, which are phloroglucinol derivatives contained in eucalyptus leaves, exhibit antimicrobial activity against a variety of bacteria including oral bacteria. this study examined effects of macrocarpals a, b, and c on periodontopathic bacteria, especially porphyromonas gingivalis. | 2006 | 16626372 |
| response of human macrophage-like cells to stimulation by fusobacterium nucleatum ssp. nucleatum lipopolysaccharide. | monocytes/macrophages are key members of the innate immune system and are present in higher numbers in active periodontal lesions than in inactive sites. the aim of this study was to characterize the response of human monocyte u937 cells, differentiated into adherent macrophages by treatment with phorbol-12-myristate 13-acetate, to stimulation by fusobacterium nucleatum ssp. nucleatum lipopolysaccharide. attachment of (3)h-lipopolysaccharide to macrophage-like cells was partially inhibited by an ... | 2006 | 16626377 |
| sodium ion pumps and hydrogen production in glutamate fermenting anaerobic bacteria. | anaerobic bacteria ferment glutamate via two different pathways to ammonia, carbon dioxide, acetate, butyrate and molecular hydrogen. the coenzyme b12-dependent pathway in clostridium tetanomorphum via 3-methylaspartate involves pyruvate:ferredoxin oxidoreductase and a novel enzyme, a membrane-bound nadh:ferredoxin oxidoreductase. the flavin- and iron-sulfur-containing enzyme probably uses the energy difference between reduced ferredoxin and nadh to generate an electrochemical na+ gradient, whic ... | 2005 | 16645308 |
| supragingival plaque may modify the effects of subgingival bacteria on attachment loss. | bacterial interactions in response to local and systemic factors may explain important features of the pathogenesis of periodontal disease. this study evaluates the role of supragingival plaque level on the relationship between subgingival microorganisms and the risk of attachment loss. | 2006 | 16671872 |
| a model of spontaneous periodontitis in the miniature goat. | the pathogenesis and progression of periodontal disease have been extensively studied through the use of animal models. however, no animal model has yet been established that is precisely similar to periodontitis in humans. in the present study, we examined the use of shiba goats as a model for spontaneous periodontitis. | 2006 | 16671878 |
| antimicrobial effect in vitro of chlorhexidine and calcium hydroxide impregnated gutta-percha points. | the aim of this study was to evaluate the antimicrobial activity of points containing antiseptics used for temporary obturation in endodontics. points containing calcium hydroxide (roeko and hygienic), and chlorhexidine (roeko) were tested and gutta-percha points served as control (meta dental corp.). the following microorganisms were studied: streptococcus mutans (sm), enterococcus faecalis (ef), staphylococcus aureus (sta), candida albicans (ca), fusobacterium nucleatum (fn) atcc 25586, porphy ... | 2005 | 16673792 |
| prevalence of periodontopathic bacteria on the tongue dorsum of elderly people. | the aim of this study was to analyse the prevalence of oral bacteria on the dorsum of the tongue. in addition, the relationship between the number of teeth and the microflora present on the coating of the tongue in a population of 85-year-old people was assessed. | 2006 | 16677187 |
| selective inhibition of porphyromonas gingivalis growth by a factor xa inhibitor, dx-9065a. | porphyromonas gingivalis is a causative bacterium of adult periodontitis. however, there is no drug specific for p. gingivalis and for its virulence factor. | 2006 | 16677284 |
| genetic diversity of oral fusobacterium nucleatum isolated from patients with different clinical conditions. | the genetic diversity of 23 oral fusobacterium nucleatum isolated from 15 periodontal patients, eight from seven healthy subjects, nine from nine aids patients and two from two cebus apella monkeys were analyzed. ecori restricted the bacterial dna and 28 ribotypes grouped from a to j groups were obtained. isolates formed 24 ribotypes which were contained into a, b, c, d, e and f groups, and three reference strains and two clinical isolates of a. actinomycetemcomitans, and e. coli cdc formed four ... | 2006 | 16699623 |
| anaerobic microbiology in 198 cases of pleural empyema: a bulgarian study. | the aim of the study was to evaluate the incidence of anaerobic bacteria in 198 patients with pleural empyema and the susceptibility of isolates to eight antibacterial agents. isolates were identified by the crystal anaerobes identification system, api system rapid id 32 a and/or routine methods. susceptibility was tested by sceptor mic system for anaerobic bacteria and limited agar dilution method. anaerobic bacteria were found in 74.2% of the patients and included 247 strains within 21 genera. ... | 2004 | 16701526 |
| anaerobes in the upper respiratory tract in infancy. | development of the indigenous microbiota begins on the surfaces of the human body after birth when infants are exposed to continuous person-to-person and environmental contacts with microbes. anaerobes constitute a significant part of indigenous bacterial communities at different body sites. pioneering anaerobic commensals are able to colonize and survive in the oral cavity during the first months of life. after teeth emerge, more attachment sites and potential niches are available for anaerobic ... | 2005 | 16701543 |
| the association of anaerobic bacteria with infectious mononucleosis. | this review summarizes the information that supports the potential importance of anaerobic bacteria in the pharyngo-tonsillitis (pt) associated with infectious mononucleosis (im). evidence supportive of the potential of involvement of bacteria in im was provided by several studies that showed increased recovery of bacteria on the tonsillar surfaces in im. several studies demonstrated that metronidazole therapy alleviated the clinical symptoms of tonsillar hypertrophy and shortened the duration o ... | 2005 | 16701590 |
| characterization of proteolytic activities of fusobacterium nucleatum. | this investigation attempted to detect the proteolytic activity of fusobacterium nucleatum in living cells, lysate cells, and supernatant of cultures. the reactions were optimized in their ph, temperature, reaction time, enzyme source, and substrate volume. synthetic substrates beta-naphthylamides (cys-na, ser-na, leu-na, glu-na, lys-na and bana), carbobenzoxy l-tirosine p-nitrophenylester (ctn), and natural substrate azoalbumin were used. reaction occurred with cys-na, ser-na, and glu-na in liv ... | 2006 | 16728241 |
| state-dependent conformations of the translocation pathway in the tyrosine transporter tyt1, a novel neurotransmitter:sodium symporter from fusobacterium nucleatum. | the gene of a novel prokaryotic member (tyt1) of the neurotransmitter:sodium symporter (nss) family has been cloned from fusobacterium nucleatum. in contrast to eukaryotic and some prokaryotic nsss, which contain 12 transmembrane domains (tms), tyt1 contains only 11 tms, a characteristic shared by approximately 70% of prokaryotic nss homologues. nonetheless upon heterologous expression in an engineered escherichia coli host, tyt1 catalyzes robust na+-dependent, highly selective l-tyrosine transp ... | 2006 | 16798738 |
| antimicrobial actions of benzimidazoles against the oral anaerobes fusobacterium nucleatum and prevotella intermedia. | benzimidazoles are widely used as proton-pump inhibitors to control stomach hyperacidity and have been found also to have antimicrobial actions against helicobacter pylori and oral streptococci. our primary aim was to determine if they are active also against oral anaerobes associated with gingivitis. our major focus was on catabolism because it leads to production of inflammatory metabolites such as butyrate and ammonia. the benzimidazoles are effective in the protonated form at acid ph values ... | 2006 | 16806047 |
| antimicrobial barrier of an in vitro oral epithelial model. | oral epithelia function as a microbial barrier and are actively involved in recognizing and responding to bacteria. our goal was to examine a tissue engineered model of buccal epithelium for its response to oral bacteria and proinflammatory cytokines and compare the tissue responses with those of a submerged monolayer cell culture. | 2006 | 16815238 |
| evaluation of 4 swab transport systems for the recovery of atcc and clinical strains with characterized resistance mechanisms. | despite limitations of swab transport systems (sts), many clinical samples and bacterial isolates are sent on these devices. we evaluated the performance of 4 commercial sts: m40 transystem (s1, copan italia, bovezzo, italy), uni-ter (s2, meus, piove di sacco, italy), euromed (s3, lab service, surbo, italy), and eurotubo (s4, deltalab, rubí, spain). survival of atcc and clinical strains with characterized resistance mechanisms, stored at room temperature and using the roll-plate method (nccls m4 ... | 2006 | 16822635 |
| effectiveness of ultrasonic instruments in the therapy of severe periodontitis: a comparative clinical-microbiological assessment with curettes. | patients with deep periodontal pockets were treated with either vector system (tg) or manual instruments (cg). clinical assessments by supragingival plaque (pl+), gingival index (gi), bleeding on probing (bop), probing depth (pd), clinical attachment level (cal) and subgingival plaque collection for microbiological analysis were made prior to and after treatment. multiplex polymerase chain reaction was used to determine the presence of actinobacillus actinomycetemcomitans, campylobacter rectus, ... | 2006 | 16841550 |
| inflammation, heat shock proteins and periodontal pathogens in atherosclerosis: an immunohistologic study. | inflammation is a significant component of atherosclerosis lesions. bacteria, including periodontopathogens, have been demonstrated in atherosclerotic plaques and cross-reactivity of the immune response to bacterial groel with human heat shock protein 60 has been suggested as a link between infections and atherosclerosis. | 2006 | 16842503 |
| an in vitro study of antimicrobial activity and efficacy of iodine-generating hydrogel dressings. | to determine the antimicrobial activity and efficacy of different formulations of novel bioxygenating hydrogel dressings (which deliver both iodine and oxygen into the wound) against various target organisms by means of an in vitro test system that more effectively mimics the conditions encountered when dressings are in contact with wounds. | 2006 | 16869198 |
| genetic and biochemical characterization of fus-1 (oxa-85), a narrow-spectrum class d beta-lactamase from fusobacterium nucleatum subsp. polymorphum. | previous studies have reported beta-lactamase-mediated penicillin resistance in fusobacterium nucleatum, but no beta-lactamase gene has yet been identified in this species. an f. nucleatum subsp. polymorphum strain resistant to penicillin and amoxicillin was isolated from a human periodontitis sample. dna cloning and sequencing revealed a 765-bp open reading frame encoding a new class d beta-lactamase named fus-1 (oxa-85). a recombinant escherichia coli strain carrying the bla(fus-1) gene exhibi ... | 2006 | 16870757 |
| protective effects of grape seed proanthocyanidins against oxidative stress induced by lipopolysaccharides of periodontopathogens. | during phagocytosis or stimulation with bacterial components, macrophages activate various cell processes, including the production of reactive oxygen species (ros) and reactive nitrogen species (rns), which are critical for successful defense against invading organisms. increased levels of ros/rns create oxidative stress that results in tissue and bone destruction. grape seed proanthocyanidins have been reported to possess a wide range of biologic properties against oxidative stress. in the pre ... | 2006 | 16881806 |
| periodontal microflora of hiv infected patients with periodontitis. | the aim of this study was to determine the microbial profile of periodontal lesions in hiv seropositive patients and to compare it with rapidly progressing periodontal lesions in systemically healthy patients. the subgingival microflora of 20 cdc ii, 20 cdc iii, 20 cdc iv/v and 20 systemically healthy patients with rapidly progressing periodontitis was examined. four sites with greatest probing depth in each patient were selected for microbiological sampling. the samples were cultured aerobicall ... | 1997 | 16887570 |
| ribotyping of bacteria from root canal and blood of patients receiving endodontic therapy. | dna restriction patterns and corresponding ribotypes of 26 bacterial isolates (propionibacterium acnes, peptostreptococcus prevotii, fusobacterium nucleatum, prevotella intermedia, prevotella nigrescens, actinomyces israelii, streptococcus intermedius, and streptococcus sanguis) recovered from patients with infected root canals and their peripheral blood, collected during and after endodontic therapy, were examined. eleven additional reference strains including type strains were also examined. p ... | 1997 | 16887597 |
| the behaviour of fusobacterium nucleatum chemostat-grown in glucose- and amino acid-based chemically defined media. | fusobacterium nucleatum is a gram-negative anaerobe, found in a number of different areas of human and animal bodies as part of the resident microbiota. however, it also appears to be involved in polymicrobial infections in such sites. it occurs in the oral cavity where it is a prominent member of various bacterial consortia associated with periodontal diseases. like most fusobacteria, it derives energy via the fermentation of amino acids which it can obtain through the dissimilation of small pe ... | 1998 | 16887630 |
| an in vitro time-kill assessment of linezolid and anaerobic bacteria. | linezolid is a novel oxazolidinone antibacterial agent active against staphylococci (including methicillin-resistant strains), enterococci (including vancomycin-resistant strains), streptococci (including penicillin-intermediate and -resistant streptococcus pneumoniae), and other aerobic and facultative bacteria. the agent has also demonstrated activity against a broad spectrum of gram-positive and gram-negative anaerobic bacteria. previous time-kill assessments have shown linezolid to be genera ... | 2003 | 16887680 |
| susceptibility of fusobacterium nucleatum to killing by peroxidase-iodide-hydrogen peroxide combination in buffer solution and in human whole saliva. | some gram-negative anaerobic bacteria have been associated with the infection of tooth supporting tissues, i.e. periodontitis. of these bacteria, fusobacterium nucleatum is sensitive to lactoperoxidase/myeloperoxidase-iodide-hydrogen peroxide system in vitro, but salivary concentrations of thiocyanate abolishes the bactericidality. these bacteria are located in periodontal pockets, on oral mucosa and in saliva. although f. nucleatum most probably does not belong to the group of main periodontal ... | 2003 | 16887684 |
| anaerobic bacteria cultured from the tongue dorsum of subjects with oral malodor. | the bacteria on the dorsum of the tongue are the most frequent cause of oral malodor; however, the bacterial flora of the tongue has not been well defined. although recent studies have used dna probes to detect the presence of certain periodontal pathogens, cultural studies have been limited because of the complexity of the flora of the tongue dorsum. the purpose of this study was to grow and to identify maximum numbers of capnophylic gram-negative bacilli and anaerobic micro-organisms by cultur ... | 2003 | 16887710 |
| characterisation and subcellular localisation of the groel-like and dnak-like proteins isolated from fusobacterium nucleatum atcc 10953. | fusobacterium nucleatum is associated with periodontitis in humans, and is a central member of the dental biofilm. heat shock proteins (hsps) of many different bacteria have been considered to play important roles during inflammations and infections. we have identified and characterised the hsp60 and hsp70, the escherichia coli groel and dnak homologues, respectively, in f. nucleatum atcc 10953. the n-terminal 22 amino acid residues of hsp60 exhibited up to 63.6% identity with members of the hsp ... | 2003 | 16887717 |
| in vitro activity of linezolid against clinical isolates of fusobacterium spp. | although most susceptibility studies for linezolid have investigated aerobic bacteria, only a few have investigated anaerobe isolates. the aim of the present study was to determine the antibacterial activity of linezolid against a larger sample of clinical isolates of fusobacterium spp. and to report on the detailed susceptibility, stratified by species. | 2006 | 16905529 |
| expression of the short fimbriae of porphyromonas gingivalis is regulated in oral bacterial consortia. | the mfa1 protein of porphyromonas gingivalis is the structural subunit of the short fimbriae and mediates coadhesion between p. gingivalis and streptococcus gordonii. we utilized a promoter-lacz reporter construct to examine the regulation of mfa1 expression in consortia with common oral plaque bacteria. promoter activity of mfa1 was inhibited by s. gordonii, streptococcus sanguinis and streptococcus mitis. in contrast, streptococcus mutans, streptococcus cristatus, actinomyces naeslundii, actin ... | 2006 | 16907740 |
| influence of residual bacteria on periapical tissue healing after chemomechanical treatment and root filling of experimentally infected monkey teeth. | the purpose of this study was twofold: first, to determine the influence on the healing of the periapical tissues when selected bacterial strains and combinations thereof remain after root canal treatment; and, second, the relationship to healing of the quality of the root filling. in eight monkeys, 175 root canals, previously infected with combinations of four or five bacterial strains and with radiographically verified apical periodontitis, were endodontically treated, bacteriologically contro ... | 2006 | 16911098 |
| association of eubacterium nodatum and treponema denticola with human periodontitis lesions. | the purpose of the present investigation was to compare the levels, proportions and percentage of sites colonized by 40 bacterial species in subgingival plaque samples from periodontally healthy subjects and patients with chronic periodontitis to seek possible pathogens other than the consensus pathogens porphyromonas gingivalis and tannerella forsythia. | 2006 | 16922925 |
| oral adhesion and survival of probiotic and other lactobacilli and bifidobacteria in vitro. | most probiotic products are consumed orally and hence it is feasible that the bacteria in these products may also attach to oral surfaces; however, the effects of these bacteria on the oral ecosystem are mostly unknown. our aim was to evaluate the oral colonization potential of different probiotic, dairy, and fecal lactobacillus and bifidobacterium strains in vitro. | 2006 | 16922933 |
| antibacterial effect of electrolyzed water on oral bacteria. | this study investigated the antibacterial effect of electrolyzed water on oral bacteria both in vitro and in vivo. tap water was electrolyzed in a water vessel using platinum cell technology. the electrolyzed tap water (called puri-water) was put in contact with five major periodontopathogens or toothbrushes contaminated with these bacteria for 30 sec. in addition, puri-water was used as a mouthwash for 30 sec in 16 subjects and the antibacterial effect on salivary bacteria was evaluated. puri-w ... | 2006 | 16953177 |
| periodontal therapy in siblings with papillon-lefèvre syndrome and tinea capitis: a report of two cases. | report of clinical and microbiological periodontal findings before and 6 months after treatment of two siblings with papillon-lefèvre syndrome (pls) and tinea capitis. | 2006 | 16970621 |
| coaggregation interactions between oral and endodontic enterococcus faecalis and bacterial species isolated from persistent apical periodontitis. | interactions between enterococcus faecalis and other species found in root canal infections might be important for the development and persistence of periapical disease. the aim of this study was to investigate the coaggregation interactions between e. faecalis clinical isolates and species previously shown to survive and induce apical periodontitis in monkeys: peptostreptococcus anaerobius, prevotella oralis, fusobacterium nucleatum, and streptococcus anginosus. intergeneric coaggregation assay ... | 2006 | 16982270 |