Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| incidence and elimination of r plasmids in vibrio cholerae. | of 124 strains of vibrio cholerae, 32 were multiply resistant to antibiotics. this resistance appeared to be determined by r plasmids on the basis of their effective elimination by sodium dodecyl sulfate, acridine orange, ethidium bromide, and ultraviolet radiation. | 1977 | 879755 |
| heterogeneity of purified cholera toxin. | the heterogeneity of vibrio cholerae toxin, obtained from culture filtrates in homogeneous form by gel filtration and preparative disc gel electrophoresis has been studied. by means of disc electrophoresis on polyacrylamide gel cholera toxin was separated into three forms designated i (5%), ii (15%) and iii (80%). the toxic activity, amino acid content and molecular weight of the three forms were similar. the difference so far observed between the various electrophoretic fractions is a differenc ... | 1977 | 884132 |
| macrophage plasminogen activator: induction by concanavalin a and phorbol myristate acetate. | the synthesis and secretion of plasminogen activator by cultured macrophages can be induced and stimulated by concanavalin a and by phorbol myristate acetate, and inhibited by such agents as glucocorticoids, mitotic inhibitors and compounds affecting camp metabolism. by the manipulation of stimulatory and inhibitory influences, enzyme production can be modulated continuously over a 200 fold range. in the same way, the proportion of cells that secrete detectable levels of enzyme can be varied fro ... | 1977 | 884740 |
| enhanced non-electrolyte uptake in vitro by the dog jejunal mucosa in the cholera-toxin-induced secretory phase. | a characteristic secretory response in the dog jejunumis observed following three hours' perfusion with cholera toxin. when this mucosa in the secretory phase is examined in vitro, the steady-state accumulation of phenylalanine and beta-methyl-glucoside is considerably enhanced. since the initial rates of influx of these substrates are unchanged and the tissue ion contents are the same as normal, it is concluded that the increase in steady-state uptake may be attributed to a change in the rate o ... | 1977 | 886482 |
| study of intestinal immunity against v. cholerae: role of antibody to v. cholerae haemagglutinin in intestinal immunity. | cell-bound haemagglutinin as an adhesive factor of vibrio cholerae has been partially purified from e1 tor vibrios using 0.05 m cyclohexylaminopropane sulfonic acid buffer ph 9.5 and gel filtration column chromatography. rabbits were immunized with the precipitin complexes of the haemagglutinin and its antibody. the antiserum is tested for the protective ability against the oral challenge with vibrio cholerae strain of which the haemagglutinin has been prepared. the results indicate definite pro ... | 1977 | 887992 |
| simple method for purifying choleragenoid, the natural toxoid of vibrio cholerae. | choleragenoid, a nontoxic aggregate of the b subunit of cholera toxin, has been purified from concentrated culture filtrates in a single step by ion-exchange chromatography on phosphocellulose or other cation-exchange resins. this procedure is far simpler than others currently used to isolate choleragenoid and yields a preparation essentially free from nucleic acid, lipopolysaccharide, toxin, and other proteins present in the crude culture filtrates. the purified choleragenoid retained the speci ... | 1977 | 892899 |
| immune response of the intestinal mucosa to cholera toxoid. | the intestinal immune response to cholera toxoid was studied in dogs and rats. oral or intraperitoneal priming followed by duodenal boosting with toxoid reulted in antitoxin-containing plasma cells (acc) in jejunal lamina propria of rats. priming and boosting by the intraperitoneal route alone induced almost no jejunal response. lamina propria acc were derived largely from migrating immunoblasts, which appeared earlier among thoracic duct lymphocytes. protection of dogs after repeated subcutaneo ... | 1977 | 894085 |
| enterotoxicity of aeromonas hydrophila. | live cells and cell-free culture supernates of 50 strains of aeromonas hydrophila isolated from diarrhoeic and healthy human faeces, drinking water, sewage, the river ganges and faeces from domestic animals caused accumulation of fluid in ligated ileal loops of adult rabbits. the amount of fluid produced was comparable to that of a toxigenic strain of vibrio cholerae. three of the strains gave positive reactions only after two passages in ileal loops of rabbits. inocula of about 10(3) viable cel ... | 1977 | 894697 |
| interaction of vibrio cholerae el tor and gut mucosa in ligated rabbit ileal loop experiment. | light and electron microscope studies in ligated rabbit gut loops showed that cholera vibrios el tor attached themselves to the enterocytes and multiplied, most probably on the intestinal epithelium surface. but they did not penetrate deeper and were unable to cause destruction of the epithelium or marked inflammation. ultrastructural manifestations of both apocrine and merocrine secretion of the enterocytes were observed. in addition, the enterocytes showed signs of generally enhanced activity. ... | 1977 | 895212 |
| [interactions of the cholera vibrion and its toxins with the body on the ultrastructural level]. | 1977 | 895581 | |
| [classification of acute bacterial enteropathies]. | the first part considers pathogenic microorganisms (vibrio cholerae and parahaemolytic vibrio, clostridium welchii, enteropathogenic e. coli, shigella, salmonella, other enterobacteria and pseudomonas. yersinia, simply enterotoxic staphylococcus and that producing acute enteritis) and the process of infection (formation of a surface link without endocellular penetration with elaboration of hexotoxins, formation of a surface link with subsequent intracellular penetration, submucosa penetration). ... | 1977 | 896078 |
| cholera toxin crystals suitable for x-ray diffraction. | large crystals of the cholera toxin were grown; their dimensions, symmetry (p21), order, and resistance to radiation make them ideally suited for a high-resolution x-ray structure determination. there is one molecule (approximately 84,000 daltons) per asymmetric unit, and therefore the lattice reveals no molecular symmetry. two distinct bioassays indicate that the protein from dissolved crystals retains full biological activity. | 1977 | 897666 |
| [electron microscopic study of the structure of cholera bacteriophage serotype ii]. | the methods of electron microscopy and optic difraction were used to determine the main parameters of the structural organization of cholera bacteriophage of serotype ii. the virions of the phage under study were found to consist of a capsid of icosahedral shape and 77.4 x 66.1 nm in size, formed of 252 morphological subunits and having the cavity volume of 76.3 x 10(3) nm3. a process of 94.8 nm in length adheres to the capsid, with a shell capable of contractions, of 85.6 nm in length, 20.6 nm ... | 1977 | 898900 |
| [role of adrenal cortex hormones in the realization of the biological action of cholerogen]. | experiments were conducted on albino mice, guinea pigs, and rabbits. the role played by glucocorticoids in the realization of the biological action of cholerogen--cholera exotoxin (edema of the limbs in mice and the skin test after craig in guinea pigs and rabbits) was studied. tissue sensitivity of these animals to the action of cholerogen diminished under the effect of stress factors (intramuscular injection of formalin, intraperitoneal injection of cholerogen, immobilization, hunger, laparato ... | 1977 | 899424 |
| [comparison of the dna polynucleotide sequence of cholera so-called non-agglutinating vibrios]. | 1977 | 899451 | |
| [restoration of the agglutinability of cholera vibrios in the bodies of guinea pigs]. | 1977 | 899452 | |
| cross linking of deoxyribonucleic acid in furazolidone treated vibrio cholerae cell. | 1977 | 901562 | |
| [the 7th cholera pandemic]. | 1977 | 904730 | |
| vibrio cholerae, vibrio parahaemolyticus, and other vibrios: occurrence and distribution in chesapeake bay. | vibrio cholerae was isolated at several locations in chesapeake bay in fall 1976 and spring 1977. strains induced fluid accumulation in rabbit ileal loops and positive activity in y-1 adrenal cells. vibrio cholerae, vibrio parahaemolyticus, and related vibrios show a spatial and temporal distribution characteristic of vibrio species in an estuary. the vibrio cholerae strains isolated from chesapeake bay represent serotypes other than o-group i--that is, so-called nonagglutinable vibrios--and are ... | 1977 | 910135 |
| mitogenic action of neuraminidase. | a new effect of ncv on lymphocytes is demonstrated. this property is the capacity to act as a mitogen in and of itself. the possible mechanisms of this phenomenon are discussed. | 1977 | 923734 |
| transformation of vibrios with r factors from enterobacteria. | 1977 | 924579 | |
| pathogenic mechanisms of a non-agglutinable vibrio cholerae strain: demonstration of invasive and enterotoxigenic properties. | a non-agglutinable vibrio cholerae strain isolated from the blood of a child with kwashiorkor and fever was shown to have the potential to invade as well as to produce a toxin resembling cholera toxin. | 1977 | 924682 |
| comparative antigenicity testing of cholera vaccines by application of hog gastric mucin and ferric ammonium citrate. | 1977 | 925044 | |
| assay of cholera vaccine potency. | 1977 | 925045 | |
| susceptibilities of bacteria against chemotherapeutics under different conditions. | 1977 | 926525 | |
| age related differences in binding of concanavalin a to plasma membranes of isolated neurons. | neurons isolated from the lateral vestibular nucleus of young adult and senescent fischer-344 rats were incubated with fluorescamine-labelled concanavalin a (fl-con a) alone, or following incubation in trypsin or vibrio cholerae neuraminidase. they were then observed and photographed. microdensitometric analysis of fluorescence micrographs showed that senescent rat neurons were significantly more fluorescent than those from young adult rats. additionally, either patches or caps of fl-con a were ... | 1977 | 930848 |
| intestinal myoelectric activity in response to live vibrio cholerae and cholera enterotoxin. | the myoelectric response of the rabbit ileum was studied in response to live vibrio cholerae culture, a whole cell lysate of cholera, and the purified enterotoxin. each cholera preparation produced a series of highly organized migrating action potential complexes (mapc). an mapc was defined as action potential discharge with a duration of 2.5 s or longer, followed by similar activity on at least one other consecutive electrode site. the mean and modal onset time of mapc activity occurred 4 h aft ... | 1976 | 932212 |
| in vitro production of ige by human peripheral blood lymphocytes: effect of cholera toxin and beta adrenergic stimulation. | peripheral blood lymphocytes from two human donors with elevated serum ige concentrations were maintained in short term tissue culture preparations. repeated culture preparations demonstrated that ige was produced in vitro in amounts that could be measured by the double antibody radioimmunoassay technique. the amount of ige produced by replicate cultures of cells from a single bleeding of the donor was similar when the cultures were simultaneously prepared. in contrast, ige production by the sam ... | 1976 | 932435 |
| suppression of growth of vibrio cholerae in the intestine of immunized mice. | in ligated intestinal loops of actively immunized adult mice, growth of v. cholerae 569b was suppressed approximately seven fold when compared to bacterial growth in non-immune animals. similarly, growth of v. cholerae 569b was reduced in mice immunized with a hybrid vibrio strain, ncv569b-165, which shares only flagella antigens with v. cholerae 569b. immunofluorescence studies of intestinal loop contents and intestinal sections indicated that, in non-immune mice, vibrios coated the intestinal ... | 1976 | 934024 |
| interaction of cholera toxin and ganglioside g(m1). | the exotoxin produced by vibrio cholerae is rapidly and firmly bound to the outer membrane of mammalian cells. with simple in vitro and in vivo methods and very pure gangliosides and allied glycolipids we have demonstrated that the monosialosylganglioside gm1 is the natural receptor for the cholera toxin. this ganglioside binds the toxin with a high affinity and inactivates it. the inactive derivative, choleragenoid toxoid has the same affinity to gm1 as the toxin. ganglioside gm1 was isolated f ... | 1976 | 937147 |
| the role of gangliosides in the action of cholera toxin. | 1976 | 937148 | |
| stimulated flow of pancreatic and biliary secretions after intestinal exposure to cholera toxin. | 1. duodeno-jejunal intestinal loops in rats were perfused with cholera toxin and the pancreatic and biliary secretory responses studied. 2. intraluminal cholera toxin induced a significant flow of pancreatic and biliary fluid and sustained protein (enzyme) secretion in pancreatic juice, in addition to the expected stimulation of mucosal secretion. 3. intravenously injected cholera toxin failed to elicit a secretory flow from the pancreas and biliary tract. 4. the time-course of the enhanced secr ... | 1976 | 939069 |
| [a new method of isolating pure cultures of the cholera vibrio. iii]. | 1976 | 941603 | |
| cholera toxin inhibits interferon action. | 1976 | 942430 | |
| structure and specificity of gangliosides. | 1976 | 945662 | |
| [choleragen and neuraminidase production by vibrio in vitro]. | in vitro studies demonstrated a high degree of the rank correlation between the synthesis of cholera exotoxin and neuraminidase by cholera vibrios (v. cholerae 569b). the appearance of these biochemically-active materials proved to depend on the growth phase of the microbial population. a possibility of cooperation between them in the pathogenesis of cholera is suggested. | 1976 | 947378 |
| [model of a chronic vibrio cholerae carrier based on gnotobiotic rats]. | germ-free monoflora (contaminated with nonpathogenic spore-bearing bacillus) and common albino rats (ofa) were infected with v. cholera el tor, of ogava and inaba serological types (6 milliard microbial cells per 1.5 ml of physiological solution per rat). approximately one week after the infection the number of vibrios reached hundreds of millions per 1 g of feces and persisted at this level for over 100 days (observation period). newborn rats were infected by natural way from adult vibrio carri ... | 1976 | 947380 |
| inactivation of the biological activities of the thermostable direct hemolysin of vibrio parahaemolyticus by ganglioside gt1. | biological activities of the thermostable direct hemolysin produced by vibrio parahaemolyticus, such as its hemolytic activity and lethal activity, were inhibited by neuraminidase-sensitive gangliosides, of which gt1 ganglioside was the most inhibitory. neuraminidase-resistant gangliosides did not affect the activities of the hemolysin. results showed that horse erythrocytes, which are resistant to the hemolysin, do not contain the neuraminidase-sensitive gangliosides gt1 and gd1a. therefore, we ... | 1976 | 947841 |
| uptake and metabolism of gangliosides in transformed mouse fibroblasts. relationship of ganglioside structure to choleragen response. | nctc 2071 cells, transformed mouse fibroblasts, when grown in chemically defined medium, are deficient in gangliosides and do not respond to choleragen. the cells lack two biosynthetic enzymes, cmp-sialic acid:lactosylceramide sialyltransferase and udp-galactose:gm2 (galnac-[acneu]-gal-glc-ceramide) galactosyltransferase, which are required for ganglioside synthesis. following uptake of ganglioside gm1 (gal-galnac-[acneu]-gal-glc-ceramide) from the medium, the cells respond to choleragen; howeve ... | 1976 | 947892 |
| [polyvalent diagnostic cholera phage]. | a polyvalent diagnostic bacteriophage for rapid identification (in 6-10 hours) of cholera vibrio of both biotypes has been constructed of virulent e1 tor phages and recommended for laboratory practice. polyvalent diagnostic bacteriophage is strictly specific, inactive against the nonagglutinating vibrios, microbes of enteric group and those closely affiliated. | 1976 | 948962 |
| [study of the fractional makeup of diagnostic nutrient media for isolating vibrio cholerae]. | a study was made of the fractional composition of peptones used for preparation of solid nutrient media. it was shown that the best growth of the vibrio on these media was obtained with the use of highly split peptones. examination of fractional composition of peptone by gel-filtration and fractionation with various precipitants is recommended for rational working out of nutrient media for cultivation of cholera vibrio. | 1976 | 948963 |
| effect of growth temperature and culture age on the lipid composition of vibrio cholerae 569b (inaba). | the relative amounts of the major phospholipids (phosphatidylethanolamine, phosphatidylglycerol and lyso-phosphatidylethanolamine) and fatty acids in vibrio cholerae 569b (inaba) varied with growth temperature and between exponential and stationary phases of growth. | 1976 | 950557 |
| [study of the ultrastructure of various representatives of the genus vibrio in relation to toxicogenesis]. | 1976 | 950927 | |
| a bioptic study of gastrointestinal mucosa in cholera patients during an epidemic in southern italy. | a histological biopsy study of gastric and jejunal mucosa of eight acute cholera patients during an epidemic in southern italy was carried out. the study demonstrated in all patients an intact epithelial lining of gastric and jejunal mucosa, a moderate degenerative process of enterocytes, presence of inflammatory lesions manifested by edema, vascular congestion, mononuclear cell infiltrate of lamina propria, and discharge of goblet-cells mucus. these changes reverted to normal in a few days. the ... | 1976 | 952275 |
| [the dehydrogenation of butandiol by "vibrio parahaemolyticus" and other vibrios (author's transl)]. | the butandiol dehydrogenase was examined in 64 vibrios isolated in togo 1970/71. this enzyme was found in all of the not halophilic strains and in the species vibrio alginolyticus, whereas the species v. parahaemolyticus exhibited as well dehydrating as not dehydrating strains. the butandiol dehydrogenase could be a good marker for epidemiologic analysis in characterizing v. parahaemolyticus strains isolated from different sources, especially if no antisera are available. | 1976 | 952445 |
| a case of cholera in kingston, ont. | a case of cholera occurred in kingston, ont. in 1974 in a traveller from south africa. treatment, based on an understanding of the pathophysiology of cholera diarrhea and the mechanism of action of the vibrio cholerae enterotoxin on gastrointestinal fluid loss, consisted of correcting the severe loss of fluid and electrolytes and the metabolic acidosis, as soon as the patient could tolerate taking fluids orally, further fluid replacement consisted increasingly of oral administration of glucose a ... | 1976 | 953912 |
| laboratory investigation and infection control of cholera in kingston, ont. | vibrio cholerae, biotype el tor, was isolated in a hospital laboratory in kingston, ont. in 1974. confirmation and complete identification by the ontario regional and provincial public health laboratories was obtained within 3 days. institution of well established infection-control and public health measures prevented spread of the infection within the hospital and the community. | 1976 | 953913 |
| cholera: synopsis of clinical aspects and principles of treatment. | cholera varies greatly in clinical severity; the mortality of untreated severe cholera may be as high as 60% the main clinical feature is dehydration; fluid lost in the stools may amount to 60/. rehydration is the cornerstone of treatment. the amount of fluid required is approximately 10% of body weight in severe dehydration and 5 to 8% in moderate dehydration. fluid therapy, which must be individualized, may be successful on its own, but chemo-therapy shortens the duration of illness. tetracycl ... | 1976 | 953914 |
| bacterial proteinaceous products (bacteriocins) as cytotoxic agents of neoplasia. | several bacteriocins, bacterial proteinaceous antibiotics, are shown to markedly inhibit the division of various established (neoplastic) mammalian cell lines. the bacteriocins tested originated from escherichia coli, pseudomonas aeruginosa, vibrio cholerae, and vibrio eltor. using exponentially growing l60t mouse fibroblasts, the inhibitory effect was concentration dependent, and a growth inhibitory unit, equivalent to cytotoxic index 50, was established. expression of toxicity as a function of ... | 1976 | 953984 |
| effect of cholera toxin on intramembranous particles of a murine lymphoid cell line. | 1976 | 954857 | |
| resistance of purified cholera toxin to enzymatic treatment with pancreatic elastase and papain. | treatment of cholera toxin with pancreatic elastase and papain in vitro showed a high resistance of the toxin molecule to these enzymes, under non-denaturing conditions or in the presence of 2 m urea. these experiments support the hypothesis of a particularly stable molecular structure of the toxin, as an explanation of its activity in the intestinal lumen where the pancreatic proteases are active. | 1976 | 955031 |
| possibilities of immunization against cholera and related enterotoxic enteropathies. | scientifically controlled field studies have established that parenterally administered killed vibrio vaccines or somatic antigen preparations offer only limited degrees of protection in certain population groups and have made it obvious that new approaches to the immunoprophylaxis of cholera are needed. it has now also been established that the symptoms of cholera result from the action of the cholera enterotoxin (choleragen) on the epithelial cells of the small intestine. immulogically relat ... | 1976 | 955264 |
| chemical composition & fatty acid pattern of lipopolysaccharides from vibrio cholerae. | 1976 | 955660 | |
| role of mg2+ & fe3+ on the reversal of ni2+ toxicity in inaba serotypes of vibrio cholerae & vibrio eltor. | 1976 | 955689 | |
| the effects of sodium ricinoleate on small intestinal function and structure. | the mechanism of hydroxy fatty acid-induced secretion was investigated in perfused hamster small intestine in vivo. sodium ricinoleate at an 8-mm concentration resulted in not only secretion of water and sodium, but an increase in intestinal clearance of inulin and a 16,000 mol wt dextran as well. a concentration of ricinoleate (2 mm) which did not affect water transport, however, did not alter intestinal permeability. ricinoleate-induced intestinal secretion was also accompanied by increased mu ... | 1976 | 956372 |
| cholera toxin-peroxidase: changes in surface labeling of glioblastoma cells with increased time in tissue culture. | cholera toxin coupled to peroxidase yielded a highly specific ultrastructural marker of plasma membrane monosialogangliosides. studies with cultures of brain and brain tumors suggested that long-term culture of tissue in monolayers results in eventual loss of surface monosialogangliosides. | 1976 | 959817 |
| cholera toxin-peroxidase: changes in surface labeling of glioblastoma cells with increased time in tissue culture. | cholera toxin coupled to peroxidase yielded a highly specific ultrastructural marker of plasma membrane monosialogangliosides. studies with cultures of brain and brain tumors suggested that long-term culture of tissue in monolayers results in eventual loss of surface monosialogangliosides. | 1976 | 959818 |
| [patterns in the body's immune response to administration of el tor vibrio cholera toxin to experimental animals]. | a study was made of some regularities attending the mechanisms of antibody production in the organism of experimental animals after the administration of el tor cholera vibrio toxin. as revealed, the indices of the immune response depended on the chosen model, the method of its determination, the method of administration and the amount of the toxin administered. dynamics of the primary and secondary responses of the immunocompetent cells of mouse spleen was determined. with the action of the el ... | 1976 | 961236 |
| [identity of the h-antigen in cholera and nag vibrios]. | 1976 | 961255 | |
| [effect of cholerogen on tissue culture cells]. | a study was made of various series of cholerogen on the cultures of human continuous cells of normal (f1, rh) and tumour nature (hela). cholerogen proved to produce a marked toxic action on the cultures of f1, rh and hela cells causing a reduction of the number of living cells, depression of mitotic activity, a reduction of the intensity of staining on the sum total protein and rna, a reduction of the activity in the cells of acid phosphatase and succinic dehydrogenase, and also a reduction of p ... | 1976 | 961265 |
| fluorometric assay of sialic acid in the picomole range: a modification of the thiobarbituric acid assay. | 1976 | 962090 | |
| photoreactivating property of the vibrio cholerae cell system. | ultraviolet-inactivated chlora phage pl163/10 underwent a maximum of abot 36% of photoreactivation within the host v. cholerae cells. uv-inactivated but non-infected v. cholerae cells also underwent the same degree of photoreactivation. | 1976 | 963629 |
| demonstration of neuraminidase activity in human blood serum and human milk using a modified, radioactively labelled alpha1-glycoprotein as substrate. | n-acetylneuramini acid of alpha1-glycoprotein was oxidized with a small molar excess of periodate and reduced with tritium-labelled borohydride. by this method about 50% of the n-acetylneuraminic acid was converted to its radioactively labelled c8-analog and 25% to its c7-analog. using this modified alpha1-glycoprotein as substrate, minimum neuraminidase concentrations of 10(-18) units/ml, related to the activity of neuraminidase from vibrio cholerae, could be determined. neuraminidase activity ... | 1976 | 964915 |
| enhancement by sodium chloride of the selectivity of thiosulfate citrate bile salts sucrose agar for isolating vibrio cholerae biotype el tor. | in this study we utilized the salt-tolerant characteristics of vibrios to develop a more selective medium by addition of nacl to thiosulfate citrate bile salts sucrose (tcbs) agar. the effect of adding salt to tcbs agar varied greatly among brands of tcbs agar and between lots of the same brand. the addition of salt at concentrations as high as 1.5% (2.5% total nacl) caused the inhibition of growth of three species of commonly encountered normal bowel flora and one strain of classical vibrio cho ... | 1976 | 965476 |
| [epidemiological characteristics of cholera e1-tor]. | 1976 | 968590 | |
| [isolation and biological characterisitcs of l-forms of cholera vibrios]. | 1976 | 969878 | |
| effects of cholera toxin on cellular and paracellular sodium fluxes in rabbit ileum. | the diarrhea observed in patients with cholera is known to be related to secretion of water and electrolytes into the intestinal lumen. however, the exact mechanisms involved in these secretory processes have remained unclear. although it is clear that purified toxin acts on epithelial cell metabolism, its activity on na+ transport across intestinal mucosa is equivocal: reported either to prevent net na+ absorption or to cause net secretion of na+ from serosa to mucosa. since total transmural na ... | 1976 | 971435 |
| cell-mediated immunity to vibrio cholerae with ribonucleic acid-protein fractions of v. cholerae l-form lysates. | different l-form lysate vaccines of vibrio cholerae serotypes ogawa and inaba and their combination along with ethyl alcohol-precipitated ribonucleic acid (e-rna) and phenol-extracted rna (p-rna) fractions of v. cholerae ogawa lysates were tested for production of cell-mediated immunity. both e-rna and p-rna fractions induced an increase in leukocyte migration inhibition, macrophage migration inhibition, and macrophage aggregation. they also induced delayed hypersensitivity in rabbits. more cons ... | 1976 | 971951 |
| vibrio cholerae adherence and colonization in experimental cholera: electron microscopic studies. | colonization of the intestinal epithelium by vibrio cholerae was examined in two model systems, in ligated ileal loops of adult rabbits and in the patent gut of infant rabbits, using both scanning and transmission electron microscopy. time studies in the adult model showed a lag period of up to 1 h before the attachment of significant numbers of the vibrios. the bacteria appeared initially in small patches on the sides of the villi, predominantly along the transverse furrows. the number of adher ... | 1976 | 971962 |
| primary structure of the b subunit of cholera enterotoxin. | 1976 | 976480 | |
| bacteriological studies of cholera in visakhapatnam region of andhra pradesh. | 1976 | 977064 | |
| colonization of ascaris lumbricoides by v. cholerae. | 1976 | 978375 | |
| immunotherapy and chemotherapy in children with neuroblastoma. | recent advances with immunotherapy in animal tumors suggested that trials with a combination of chemotherapy and immunotherapy in human malignant tumors might be worthwhile. a pilot program with vibrio cholera neuraminidase-treated tumor cells plus bcg was tested in 3 patients who had had chemotherapy for disseminated neuroblastoma. two of these children were in "complete remission" after radiation therapy and chemotherapy before the administration of immunotherapy. relapse occurred in 5-6 month ... | 1976 | 978774 |
| [non-agglutinable vibrios (nag-vibrios) and cholera (author's transl)]. | 1976 | 979518 | |
| [neuraminidase as a pathogenicity factor in microbial infections (proceedings)]. | 1976 | 983495 | |
| the cholera enterotoxin--robert koch revisited [proceedings]. | 1976 | 983499 | |
| activation by cholera toxin of adenylate cyclase solubilized from rat liver. | cholera toxin, or peptide a1 from the toxin, activates adenylate cyclase solubilized from rat liver with lubrol px, provided that cell sap, nad+, atp and thiol-group-containing compounds are present. the activation is abolished by antisera to whole toxin, but not to subunit b. | 1976 | 985419 |
| adhesive properties of vibrio cholerae: adhesion to isolated rabbit brush border membranes and hemagglutinating activity. | adhesion of vibrios to the small intestine may occur (i) by association of the bacteria with secreted mucus gel or (ii) by adherence of the bacteria to the surface of epithelial cells. in the present study, vibrios readily adhered to isolated brush border membranes obtained from rabbit intestinal epithelial cells. adhesion was temperature dependent and required the presence of divalent cations such as calcium. the agglutination of human o erythrocytes by vibrio cholerae was observed also, and th ... | 1976 | 985804 |
| adhesive properties of vibrio cholerae: nature of the interaction with isolated rabbit brush border membranes and human erythrocytes. | nonmotile vibrio mutants lacked the ability to adhere to rabbit intestinal brush border membranes and to agglutinate human group o erythrocytes, but motile revertant vibrios isolated from such strains expressed adhesiveness equivalent to that of the original parent. two possible explanations for the relation between vibrio motility and adhesion in these assays systems are (i) that the rate of adhesion depends on the rate of chance contact brought about by motility, and (ii) that the flagellum ei ... | 1976 | 985805 |
| biochemistry of vibrio cholerae virulence: purification of cholera enterotoxin by preparative disc electrophoresis. | procedures for cholera enterotoxin purification previously developed in this labarotory were not applicable to large-scale purification, and these methods resulted in low yields of pure toxin. an efficient scheme has been developed whereby pure cholera enterotoxin can be obtained from 6 to 8 liters of culture supernatant fluid. this method consists of concentration by membrane ultrafiltration followed by gel filtration and cation-exchange chromatography. pure cholera enterotoxin of high biologic ... | 1976 | 987751 |
| practical estimation of the actual laboratory diagnostics of cholera. | 1976 | 990543 | |
| active and passive immunity to cholera toxin. | 1976 | 992728 | |
| determination of molecular weight of vibrio cholerae genome dna from renaturation rate. | 1976 | 992812 | |
| experimental development of antibiotic resistance in vibrio cholerae. | 1976 | 992839 | |
| immunological control mechanism against cholera toxin: interference with toxin binding to intestinal receptors. | the immunological control mechanism against cholera toxin (ct) in the small intestine of rats was studied in vivo. ct binding to intestinal receptors was determined by injected radiolabeled ct into the loops of rat small intestine and subsequently separating purified microvillus membranes from mucosal scrapings of those loops. substantial radioactivity (10(5) cpm/mg of microvillus protein) was present in microvillus fractions of small intestine exposed to 125i-labeled ct compared to radioactivit ... | 1976 | 992868 |
| an investigation of alternatives to hog gastric mucin as virulence-enhancing agents in the cholera vaccine potency assay. | 1976 | 993220 | |
| preventing insect diapause with hormones and cholera toxin. | 1976 | 994697 | |
| [sensitivity to antibiotics of el tor cholera vibrions]. | 1976 | 999265 | |
| the immunologic control mechanism against cholera toxin. ii. stimulation of adenylate cyclase without fluid secretion. | 1976 | 999681 | |
| cholera toxin and adenylate cyclase: properties of the activated enzyme in liver plasma membranes. | adenylate cyclase (ec 4.6.1.1) activity in mouse liver plasma membranes is increased fivefold when animals are pretreated with cholera toxin. the increase in activity is detectable within 20 min of an intravenous injection of the toxin. the response of the control and cholera-toxin-activated adenylate cyclase to hormones, gtp, and naf is complex. gtp causes the same fold stimulation of control and toxin-activated cyclase, but glucagon and naf remain the most potent activators of liver adenylate ... | 1976 | 1000362 |
| [case of contamination of the karst underground mineral-water stratum with vibrio cholerae]. | 1976 | 1001931 | |
| studies of the ligand binding to cholera toxin, i. the lipophilic moiety of sialoglycolipids. | the fixation of cholera toxin by ganglioside ggtet1 is dependent on the nature of the carbohydrate as well as the lipid moiety of the glycolipid. the role of the lipid in binding to the toxin investigated with synthetic ganglioside analogues (gangliosidoides). the interaction between glycolipid and toxin was followed by precipitate formation, by inhibition of toxicity and in polyacrylamide gel electrophoresis. for specific precipitation, an aliphatic hydrocarbon chain at least 14 c-atoms in leng ... | 1976 | 1002130 |
| lipolytic action of cholera toxin on fat cells. re-examination of the concept implicating gm1 ganglioside as the native membrane receptor. | the possible role of galactosyl-n-acetylgalactosaminyl-[n-acetylneuraminyl]-galactosylglucosylceramide (gm1) ganglioside in the lipolytic activity of cholera toxin on isolated fat cells has been examined. analyses of the ganglioside content and composition of intact fat cells, their membranous ghosts, and the total particulate fraction of these cells indicate that n-acetylneuraminylgalactosylglucosylceramide (gm3) represents the major ganglioside, with substantial amounts of n-acetylgalactosamin ... | 1976 | 1002701 |
| [the protective and immunodepressive activity of cell fractions of a cholera-like vibrio]. | a study was made of the protective and immunodepressive activity of the sytoplasmic fractions of a cholera-like vibrio. ribosomal fraction proved to possess more marked protective and immunodepressive properties than the soluble cytoplasmic fraction. | 1976 | 1007718 |
| [a study of immunological efficacy of the oral chemical cholera vaccine on experimental animals]. | three fractions isolated from the culture fluid of the 569b cholera vibrio strain were studied. the fractions differed by the extent of purification, and the content of the toxid and the o-antigen. in intraintestinal application to rabbits all of them caused formation of antitoxins and vibriocidal antibodies in the blood serum. the immunizing dose of the preparations in intraintestinal administration exceeded the dose required for subcutaneous application. fraction i should be used for producing ... | 1976 | 1007720 |
| [a comparative study of ultrastructural organization of the nonagglutinating vibrios and the causative agents of cholera]. | a common plan of ultrastructural organization was revealed in a comparative study of the ultrastructure of the nonagglutinating el tor and cholera vibrios: the presence of a three-layer cell wall and cytoplasmic membrane, the presence of vesicles on the external layer of the cell wall or between the cell wall and the cytoplasmic membrane; identity of the intracytoplasmic membranous structures; the presence of intracytoplasmic inclusions in the form of granules of various size and electron densit ... | 1976 | 1007727 |
| [experimentally induced cholera in guinea pigs. i. elaboration of the method of infection]. | the method of intrapulmonary infection of guinea pigs was suggested for the assessment of the virulent properties of cholera vibrios. addition into the diluent of 10% peptone, 10% gelatine and 0.05% agar-agar led to the reduction of ld50 by over 1000 times. a specific infectious process coursing in an acute generalized form with bacteriemia and affection of the small intestine developed in the infected animals. the majority of the animals perished in 1 to 2 days. | 1976 | 1007732 |
| sequence of events in the activation of adenylate cyclase by cholera toxin. | the toxin of vibrio cholera causes fluid secretion from the small intestine by stimulation of adenylate cyclase and elevation of intracellular cyclic amp concentrations. the toxin is a protein composed of subunits responsible for binding to cell membranes and a subunit responsible for the activation of adenylate cyclase. the binding subunits (b) are non-covalently bonded to the active subunit (a). the latter is composed of two polypeptides a1 and a2 linked by a disulphide bridge. exposure of the ... | 1976 | 1009263 |
| spectrophotometric studies of base composition & helix-coil transition of vibrio cholerae dna. | 1976 | 1010578 | |
| effects of noxious stimulation of the skin on transmission through the spinocervical tract [proceedings]. | 1976 | 1011101 |