Publications

direct and indirect control of lrp on lee pathogenicity genes of citrobacter rodentium.	citrobacter rodentium is a mouse pathogen that, because of its similarities with human enteropathogenic (epec) and enterohemorrhagic (ehec) strains of escherichia coli is widely used as a model system for in vivo and in vitro studies. similarly to epec and ehec, c. rodentium carries the lee (locus of enterocyte effacement) pathogenicity island, encoding virulence factors essential for causing transmissible colonic hyperplasia in mice by attaching and effacing (a/e) lesions. expression of the gen ...	2011	22092863
Citrobacter freundii septicemia in a stranded newborn Cuvier's beaked whale (Ziphius cavirostris).	Citrobacter freundii, a gram-negative enterobacterium, may cause fatal septicemia in humans and animals. Its potential pathogenic role in cetaceans (bottlenose dolphins and beluga whales) has been hypothesized. Here we describe fatal C. freundii septicemia in a stranded newborn Cuvier's beaked whale (Ziphius cavirostris).	2011	22102682
bacterial chemotaxis modulates host cell apoptosis to establish a t-helper cell, type 17 (th17)-dominant immune response in helicobacter pylori infection.	the host inflammatory response to chronic bacterial infections often dictates the disease outcome. in the case of the gastric pathogen helicobacter pylori, host inflammatory responses result in outcomes that range from moderate and asymptomatic to more severe with concomitant ulcer or cancers. it was found recently that h. pylori chemotaxis mutants (che(-)), which lack directed motility but colonize to nearly wild-type levels, trigger less host inflammation. we used these mutants to observe host ...	2011	22106256
the rising problem of antimicrobial resistance in the intensive care unit.	abstract: mainly due to its extremely vulnerable population of critically ill patients, and the high use of (invasive) procedures, the intensive care unit (icu) is the epicenter of infections. these infections are associated with an important rise in morbidity, mortality, and healthcare costs. the additional problem of multidrug-resistant pathogens boosts the adverse impact of infections in icus. several factors influence the rapid spread of multidrug-resistant pathogens in the icu, e.g., new mu ...	2011	22112929
Bacillus subtilis-mediated Protection from Citrobacter rodentium-associated enteric disease requires espH and functional flagella.	Commensals limit disease caused by invading pathogens; however, the mechanisms and genes utilized by beneficial microbes to inhibit pathogenesis are poorly understood. The attaching and effacing mouse pathogen Citrobacter rodentium associates intimately with the intestinal epithelium and infections result in acute colitis. C. rodentium is used to model the human pathogens, enterohemorrhagic E. coli and enteropathogenic E. coli. To confirm that B. subtilis, a spore-forming bacterium found in the ...	2011	22144475
distinct compartmentalization of nf-κb activity in crypt and crypt-denuded lamina propria precedes and accompanies hyperplasia and/or colitis following bacterial infection.	citrobacter rodentium induces transmissible murine colonic hyperplasia (tmch) and variable degrees of inflammation and necrosis depending upon the genetic background. utilizing c. rodentium-induced tmch in c3h/henhsd inbred mice, we observed significant crypt hyperplasia on days 3 and 7 preceding active colitis. nf-κb activity in the crypt-denuded lamina propria (clp) increased within 24 h postinfection, followed by its activation in the crypts at day 3, which peaked by day 7. increases in inter ...	2011	22144489
Gene expression profiling in the skin of zebrafish infected with Citrobacter freundii.	Skin is considered the largest immunologically active organ, but its molecular mechanism remains unclear in fish. Here, Affymetrix Zebrafish GeneChip was used to assess gene expression in the skin of zebrafish (Danio rerio) infected with the bacterium Citrobacter freundii. The results showed that 229 genes were differentially expressed, of which 196 genes were upregulated and 33 genes were downregulated. Gene Ontology and KEGG pathway analyses indicated 88 genes significantly associated with ski ...	2012	22155693
high prevalence of qnr and aac(6')-ib-cr genes in both water-borne environmental bacteria and clinical isolates of citrobacter freundii in china.	we investigated the prevalence of qnr and aac(6')-ib-cr genes in water-borne environmental bacteria and in clinical isolates of enterobacteriaceae, as well as the subtypes of qnr. environmental bacteria were isolated from surface water samples obtained from 10 different locations in hangzhou city, and clinical isolates of citrobacter freundii were isolated from several hospitals in four cities in china. qnra, qnrb, qnrs, and aac(6')-ib-cr genes were screened using pcr, and the genotypes were ana ...	2011	22167061
Production of a conjugate vaccine for Salmonella enterica serovar Typhi from Citrobacter Vi.	A conjugate vaccine for Salmonella enterica serovar Typhi was produced by chemically linking Vi, purified from Citrobacter, to the non-toxic mutant diphtheria toxin CRM(197) via an adipic dihydrazide spacer using N-(3-Dimethylaminopropyl)-N'-ethylcarbodiimide coupling chemistry. The polysaccharide purification process was developed based on Vi precipitation from culture supernatant with cetyl trimethylammonium bromide (CTAB), solubilization of the CTA-polysaccharide salt with ethanol followed by ...	2011	22172503
the aryl hydrocarbon receptor regulates gut immunity through modulation of innate lymphoid cells.	innate lymphoid cells (ilcs) expressing the nuclear receptor rorγt are essential for gut immunity presumably through production of interleukin-22 (il-22). the molecular mechanism underlying the development of rorγt(+) ilcs is poorly understood. here, we have shown that the aryl hydrocarbon receptor (ahr) plays an essential role in rorγt(+) ilc maintenance and function. expression of ahr in the hematopoietic compartment was important for accumulation of adult but not fetal intestinal rorγt(+) ilc ...	2011	22177117
Communal microaerophilic-aerobic biodegradation of Amaranth by novel NAR-2 bacterial consortium.	A novel bacterial consortium, NAR-2 which consists of Citrobacter freundii A1, Enterococcus casseliflavus C1 and Enterobacter cloacae L17 was investigated for biodegradation of Amaranth azo dye under sequential microaerophilic-aerobic condition. The NAR-2 bacterial consortium with E. casseliflavus C1 as the dominant strain enhanced the decolorization process resulting in reduction of Amaranth in 30min. Further aerobic biodegradation, which was dominated by C. freundii A1 and E. cloacae L17, allo ...	2012	22182471
Proteomic Analysis on Acetate Metabolism in Citrobacter sp. BL-4.	Mass production of glucosamine (GlcN) using microbial cells is a worthy approach to increase added values and keep safety problems in GlcN production process. Prior to set up a microbial cellular platform, this study was to assess acetate metabolism in Citrobacter sp. BL-4 (BL-4) which has produced a polyglucosamine PGB-2. The LC-MS analysis was conducted after protein separation on the 1D-PAGE to accomplish the purpose of this study. 280 proteins were totally identified and 188 proteins were se ...	2012	22211106
chemical compositions of the essential oils of aerial parts of chamaemelum mixtum (l.) alloni.	the chemical compositions of the aerial parts essential oils of chamaemelum mixtum (l.) alloni from corsica and sardinia were investigated employing gas chromatography and gas chromatography/mass spectrometry. the structure of (z)-heptadeca-9,16-dien-7-one, a natural compound not previously described, was elucidated by gas chromatography/mass spectrometry (electron impact and chemical ionization) and one-dimensional and two-dimensional nuclear magnetic resonance spectroscopy. the variation in ch ...	2012	22225420
bugs on film: the presence of bacterial rods (citrobacter koseri) on a routine blood film in a septic immunocompromised patient with a femoral vein line.		2011	22234128
pcr detection and identification of histamine-forming bacteria in filleted tuna fish samples.	total of 14 filleted yellowfin tuna fish (thunnus albacares) sold in wholesale fish market and supermarkets in milan, italy, were purchased and tested to determine microbial count, histamine level, histamine-forming bacteria, and their ability to produce histamine in culture broth. although histamine level was less than 10 ppm, many samples showed high total viable bacterial and enterobacterial counts that reached dangerous levels after temperature abuse for short periods of time. a pcr assay ta ...	2012	22251187
coastal seawater bacteria harbor a large reservoir of plasmid-mediated quinolone resistance determinants in jiaozhou bay, china.	diversity and prevalence of plasmid-mediated quinolone resistance determinants were investigated in environmental bacteria isolated from surface seawater of jiaozhou bay, china. five qnr gene alleles were identified in 34 isolates by pcr amplification, including qnra3 gene in a shewanella algae isolate, qnrb9 gene in a citrobacter freundii isolate, qnrd gene in 22 proteus vulgaris isolates, qnrs1 gene in 1 enterobacter sp. and 4 klebsiella spp. isolates, and qnrs2 gene in 1 pseudomonas sp. and 4 ...	2012	22252223
iron availability increases the pathogenic potential of salmonella typhimurium and other enteric pathogens at the intestinal epithelial interface.	recent trials have questioned the safety of untargeted oral iron supplementation in developing regions. excess of luminal iron could select for enteric pathogens at the expense of beneficial commensals in the human gut microflora, thereby increasing the incidence of infectious diseases. the objective of the current study was to determine the effect of high iron availability on virulence traits of prevalent enteric pathogens at the host-microbe interface. a panel of enteric bacteria was cultured ...	2012	22272265