Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| proteasome inhibitor mg132 sensitizes hpv-positive human cervical cancer cells to rhtrail-induced apoptosis. | in cervical carcinogenesis, the p53 tumor suppressor pathway is disrupted by hpv (human papilloma virus) e6 oncogene expression. e6 targets p53 for rapid proteasome-mediated degradation. we therefore investigated whether proteasome inhibition by mg132 could restore wild-type p53 levels and sensitize hpv-positive cervical cancer cell lines to apoptotic stimuli such as rhtrail (recombinant human tnf-related apoptosis inducing ligand). in a panel of cervical cancer cell lines, caski was highly, hel ... | 2006 | 16287099 |
| proteolytic processing of the laminin alpha3 g domain mediates assembly of hemidesmosomes but has no role on keratinocyte migration. | laminin-5 (lm5), the major adhesion ligand of basal epithelial cells, undergoes complex extracellular proteolytic processing that influences cell adhesion and migration. in tumor cell lines, the proteolytic truncation of the c-terminal g domain of the lm alpha3 chain induces assembly of hemidesmosomes and downregulates cell migration. to define the biological functions of the alpha3 g domain processing in physiological conditions, we have expressed a series of mutant alpha3 complementary dna in ... | 2005 | 16297184 |
| integration of hpv-16 and hpv-18 dna in vulvar intraepithelial neoplasia. | vulvar intraepithelial neoplasia (vin) is a premalignant disease of the lower genital tract. the increased occurrence of high-risk human papillomavirus (hpv) infection seems to be associated with the increasing frequency of vin. integration of hpv dna into host chromosome has been hypothesized to be a critical step in the carcinogenesis of cervical neoplasia resulting in altered expression of two viral transforming genes e6 and e7. | 2006 | 16300821 |
| a novel mucosal vaccine based on live lactococci expressing e7 antigen and il-12 induces systemic and mucosal immune responses and protects mice against human papillomavirus type 16-induced tumors. | current strategies to prevent or treat human papillomavirus type 16 (hpv-16) infection are promising, but remain costly. more economical but efficient vaccines are thus needed. in this study, we evaluated the protective effects of mucosally coadministered live lactococcus lactis strains expressing cell wall-anchored e7 ag and a secreted form of il-12 to treat hpv-16-induced tumors in a murine model. when challenged with lethal levels of tumor cell line tc-1 expressing e7, immunized mice showed f ... | 2005 | 16301635 |
| self-sampling versus physician-sampling for human papillomavirus testing. | we evaluated the detection of human papillomavirus (hpv) infection using two sampling methods of cervical exfoliated cells, consisting of self-sampling of vaginal cells and cervical sampling performed by the physician. women included were 379 patients of the general population attending outpatient clinics in northern greece for routine cytological cervical dysplasia screening. hpv dna detection was similar with both sampling techniques. the hpv prevalences in self-collected samples were 4.7% and ... | 2005 | 16303065 |
| vaccination as a prevention strategy for human papillomavirus-related diseases. | childhood vaccines have had an enormously beneficial impact on human health. large-scale vaccination programs have controlled diseases associated with high morbidity and mortality such as poliomyelitis, smallpox, diphtheria, tetanus, yellow fever, pertussis, haemophilus influenzae, streptococcus pneumoniae, measles, mumps, and rubella. human papillomavirus (hpv) is a significant source of morbidity and mortality throughout the world. in the united states, hpv is the most common sexually transmit ... | 2005 | 16310136 |
| pharmaceutical and immunological evaluation of human papillomavirus viruslike particle as an antigen carrier. | we report the preparation and the immunogenicity of a conjugate vaccine obtained by chemically conjugating a variant of the extracellular peptide fragment of influenza type a m2 protein to the human papillomavirus (hpv) viruslike particle (vlp). conjugates comprised of approximately 4,000 copies of the antigenic peptide per vlp are obtained as the result of the reaction between a c-terminal cysteine residue on the peptide and the maleimide-activated hpv vlp. the resulting conjugates have an aver ... | 2006 | 16315228 |
| interaction among folate, riboflavin, genotype, and cancer, with reference to colorectal and cervical cancer. | epidemiological studies have linked low folate intake with an increased risk of epithelial cancers, including colorectal cancer and cervical cancer. riboflavin has received much less attention, but there is increasing interest in the well-established role that flavins play in folate metabolism and the possible synergy of a protective effect between these 2 vitamins. folate plays a key role in dna synthesis, repair, and methylation, and this forms the basis of mechanistic explanations for a putat ... | 2005 | 16317155 |
| cervical human papillomavirus screening among older women. | rates of acquisition and clearance of cervical human papillomavirus (hpv) during a 3-year period in women 51 years of age were compared with rates in younger women to provide data on cervical screening for women >50 years of age. paired, cytologically negative, archived cervical smears taken 3 years apart from 710 women in nottingham, united kingdom, were retrieved and tested for hpv infection with polymerase chain reaction (pcr) with gp5+/6+ primers. seventy-one (21.3%) of 333 women 51 years of ... | 2005 | 16318718 |
| dihydroartemisinin is cytotoxic to papillomavirus-expressing epithelial cells in vitro and in vivo. | nearly all cervical cancers are etiologically attributable to human papillomavirus (hpv) infection and pharmaceutical treatments targeting hpv-infected cells would be of great medical benefit. because many neoplastic cells (including cervical cancer cells) overexpress the transferrin receptor to increase their iron uptake, we hypothesized that iron-dependent, antimalarial drugs such as artemisinin might prove useful in treating hpv-infected or transformed cells. we tested three different artemis ... | 2005 | 16322232 |
| inhibition of papillomavirus protein function in cervical cancer cells by intrabody targeting. | papillomaviruses (hpvs) are a major cause of human disease, and are responsible for approximately half a million cases of cervical cancer each year. hpvs also cause genital warts, and are the most common sexually transmitted disease in many countries. despite their importance, there are currently no specific antivirals that are active against hpvs. papillomavirus protein function is mediated largely by protein-protein interactions, which are difficult to inhibit using conventional approaches. to ... | 2006 | 16324714 |
| partial trachelectomy: a new treatment choice for persistent or recurrent high grade cervical intraepithelial neoplasia. | some women with persistent or recurrent disease after repeated conization for high grade cervical intraepithelial neoplasia (cin) may desire to preserve uterus if not fertility. in such case, a partial trachelectomy may become treatment of choice for them. we undertook this prospective study to determine the feasibility of partial trachelectomy as a treatment choice for persistent or recurrent high grade cin. | 2006 | 16325243 |
| [efficacy and safety of 5% imiquimod cream in external genital warts: a 6 month follow-up evaluation]. | one of the main issues raised by the management of external genital warts is their potential risk of recurrence. however, nearly all studies assessing recurrences have been conducted with a 3 month follow-up. since the latency of the human papillomavirus is long, such 3 month follow-up might be too short to detect the true recurrence rate. we therefore conducted a study evaluating patients with external genital warts, treated with an immune response modifier, 5% imiquimod cream, and followed up ... | 2005 | 16327713 |
| chemotherapy, il-12 gene therapy and combined adjuvant therapy of hpv 16-associated mhc class i-proficient and -deficient tumours. | moderately immunogenic hpv 16-associated murine tumour cell line mimicking human hpv 16-associated neoplasms tc-1 (mhc class i(+)) and its variants, tc-1/p3c10 and tc-1/a9, with a marked down-regulation of mhc i molecules, were used to examine the effect of local interleukin 12 (il-12) gene therapy for the treatment of early tumour transplants and minimal residual tumour disease obtained after cytoreductive chemotherapy (cmrtd). experiments were designed to examine whether down-regulation of mhc ... | 2006 | 16328003 |
| expression of cell cycle-regulatory proteins, mib-1, p16, p53, and p63, in squamous cell carcinoma of conjunctiva: not associated with human papillomavirus infection. | squamous cell carcinoma (scc), the most common primary malignant tumor of the conjunctiva, has a variable clinical presentation and immunohistochemical profile. abundant cell cycles exist, including mib-1 (ki67 antigen), p16, p53, and p63, within the conjunctiva scc. this investigation first reports the expressions of cell cycle markers in scc. a retrospective study was conducted between december 1976 and june 2004, comprising 13 consecutive patients with conjunctiva scc who were treated with su ... | 2006 | 16328355 |
| high prevalence of human papillomavirus infections in urine samples from human immunodeficiency virus-infected men. | infection with human immunodeficiency virus (hiv) and the resulting immunosuppression are associated with an increased risk for human papillomavirus (hpv) persistence and related malignancies. in the present study we investigated the prevalence of hpv in urine samples from 104 hiv-infected men with low cd4+ cell counts (<100 per mm(3)) and 115 urine samples from hiv-negative men. a high prevalence of hpv dna (39.4%) was found in the hiv patients. most of the hpv types were high risk (81.4%), wit ... | 2005 | 16333078 |
| risk factors in oral and oropharyngeal squamous cell carcinoma: a population-based case-control study in southern sweden. | in the year 2002, about 275,000 inhabitants around the world developed oral cancer and over half of them will die of their disease within 5 years. oral and oropharyngeal squamous cell carcinoma (ooscc) accounts for about 1% of all cancers in sweden - which is low compared to the incidence on the indian subcontinent and in other parts of asia, where it is one of the most common forms of cancer. the incidence in sweden is increasing, however. the study comprised 80% (132/165) of all consecutive ca ... | 2005 | 16335030 |
| risk factors for cervical cancer development: what do women think? | the current cervical cancer prevention strategy is exclusively directed towards screening, without taking into account any relationship with sexual risk factors. the introduction of human papillomavirus (hpv) detection into the screening procedure implicates that we should give attention to this relationship. the aim of this study was to investigate what knowledge women have of the relation between hpv and cervical cancer. | 2004 | 16335302 |
| a theoretical discussion of the impact of stigma on psychological adjustment to having a sexually transmissible infection. | this paper provides a discussion of the utility of stigma theory as a conceptual framework for the interpretation and analysis of the psychological impact of contracting a sexually transmissible infection (sti). most particularly, it focuses on those viral infections that cannot be medically cured, such as genital herpes and genital human papilloma virus. recent research in the area of stis suggests that the stigma associated with these conditions can hinder psychosocial and sexual adjustment po ... | 2005 | 16335743 |
| human papillomavirus: burden of illness and treatment cost considerations. | the monetary and personal costs to society of human papillomavirus (hpv) infection are enormous. in order to make a comparison of different treatment methods we reviewed the entire literature on hpv treatment from january 1966 to december 2003 using medline, with particular reference to published meta-analyses, randomized controlled and comparative studies. patient-applied therapies offer patients the possibility of convenient and, on the whole, pain-free treatment. podofilox (podophyllotoxin) a ... | 2005 | 16343025 |
| the intron 1 of hpv 16 has a suboptimal branch point at a guanosine. | the branch point sequence (bps) of intron 1 of the hpv-16 was determined via rt-pcr in a cell free system, using lariat intermediates obtained by in vitro splicing reactions. we used synthetic e6/e7 transcripts and hela nuclear protein extracts to obtain the splicing intermediates. then, a divergent oligonucleotide primer set, pairing on the lariat rna that encompassed the 2'-5' phosphodiester bond formed between the 5' end of the intron and the bps, was used for cdna synthesis and pcr amplifica ... | 2006 | 16343675 |
| identification of hpv variants. | the vast majority of anogenital carcinomas are caused by high-risk human papillomaviruses (hpvs), and among western nations hpv-16 is usually the most predominant cancer-associated type. as a dna virus, hpv type 16 has a relatively stable genome that is believed to have co-evolved with its host over the millennia. nevertheless, among the "wild" populations of hpv-16 that are circulating, a large number of variants have been identified, and these may have considerably different pathogenic potenti ... | 2005 | 16350393 |
| histological analysis of cervical intraepithelial neoplasia. | a wide interobserver variation is seen even among competent histopathologists in the routine diagnosis of cervical intraepithelial neoplasia (cin). as a result, early detection of low-grade cin (cin 1) lesions, in particular, remains a major challenge both in routine diagnosis and in cervical screening. in this chapter, the salient diagnostic features of human papillomavirus infection and cin lesions are demonstrated. | 2005 | 16350395 |
| analysis of p16ink4a and integrated hpv genomes as progression markers. | most cervical cancers are preventable when the precursor lesions are detected in time. human papilloma viruses (hpvs) are the main risk factors for cervical cancer development, but there is a high percentage of healthy women infected with hpv that never develop a lesion. only a small percentage of low-grade dysplasias finally grow out to invasive cancer. several biomarkers can be used to identify lesions at risk for malignant progression. overexpression of p16ink4a is induced by the viral oncopr ... | 2005 | 16350398 |
| hpv dna detection and typing in inapparent cutaneous infections and premalignant lesions. | epidemiological studies, which address the role of human papillomavirus (hpv) in the pathogenesis of (pre)malignant cutaneous lesions, focus on the hpv b1 subgroup comprising the so-called epidermodysplasia verruciformis (ev)-associated hpv types. to detect and type hpv dna in human materials, polymerase chain reaction (pcr)-based assays are used. in this chapter, a nested, broad-spectrum pcr method using a mixture of primers and a type-specific pcr using specific primers are described. the broa ... | 2005 | 16350401 |
| propagation of infectious, high-risk hpv in organotypic "raft" culture. | the organotypic (raft) culture system has been used to develop an in vitro system that is capable of reproducing the entire human papillomavirus (hpv) life cycle, including virion morphogenesis. this system utilizes hpv-containing cell lines that are either derived from biopsies or created by the transfection of keratinocytes with hpv genomic dna. when grown as raft cultures, these lines allow for a detailed study of all stages of the viral life cycle. in this chapter, we describe in detail how ... | 2005 | 16350404 |
| analysis of adeno-associated virus and hpv interaction. | it is slowly becoming accepted that adeno-associated virus (aav) is another significant factor involved in cervical carcinogenesis. however, unlike human papillomavirus (hpv), which is positively associated with cervical cancer, aav is negatively associated with this cancer. this negative association appears to be through a direct and complex bi-directional interaction between aav and hpv. essentially all assays used for studying hpv can be used for studying the aav-hpv interaction. this is beca ... | 2005 | 16350413 |
| codon optimization of papillomavirus genes. | early and late genes of human and animal papillomaviruses show a codon composition seemingly unfavorable for expression in mammalian cells. it remains unclear how the viruses manage to achieve high levels of late gene expression during the viral life cycle. one possible solution could be that the availability of certain t-rnas changes with progressing stages of cellular differentiation. previous studies have demonstrated that modification of codon usage of papillomavirus late (l1 and l2) and ear ... | 2005 | 16350416 |
| generation and applications of hpv pseudovirions using vaccinia virus. | this chapter outlines the generation and application of human papillomavirus type 33 (hpv 33) pseudovirions. these pseudovirions are structurally indistinguishable from native virions and are therefore valuable tools for the study of papillomavirus/cell interactions. the method describes (1) the construction of vaccinia viruses recombinant for the major and minor hpv capsid proteins, l1 and l2, respectively, (2) the transfection of cos7 cells with a marker plasmid replicating to high copy number ... | 2005 | 16350418 |
| the hpv xenograft severe combined immunodeficiency mouse model. | the permissive propagation of papillomaviruses outside their natural hosts has not been possible, which is an important restriction to the study of human papillomavirus (hpv) infections and associated diseases. since the mid-1980s, several models have been described that rely on the growth of hpv in susceptible human xenografts implanted in immunodeficient mice. the severe combined immunodeficiency (scid) mouse has been particularly suited to this approach, and to reproduce reliably the macrosco ... | 2005 | 16353337 |
| p53 mediates amosite asbestos-induced alveolar epithelial cell mitochondria-regulated apoptosis. | asbestos causes pulmonary toxicity in part by generating reactive oxygen species that cause dna damage. we previously showed that the mitochondria-regulated (intrinsic) death pathway mediates alveolar epithelial cell (aec) dna damage and apoptosis. because p53 regulates the dna damage response in part by inducing intrinsic cell death, we determined whether p53-dependent transcriptional activity mediates asbestos-induced aec mitochondrial dysfunction and apoptosis. we show that inhibitors of p53- ... | 2006 | 16357363 |
| [the prevalence of e6/e7 hpv type 16 in laryngeal cancer and in normal mucosa]. | many authors suggest that hpv infection can play a great role in development of benign and malignant tumours of upper respiratory tract in human. the aim of this study was to determine the prevalence of e6/e7 hpv-16 in laryngeal squamous cell carcinoma, and normal laryngeal mucosa, and to analyse their correlation with sex, lymph node status, primary tumor stage, localization, and histological differentiation. hpv 16 dna presence was analysed using pcr technique in 72 samples of laryngeal carcin ... | 2005 | 16358846 |
| mutations in the mitochondrial dna d-loop region are frequent in cervical cancer. | mitochondrial dna (mtdna) is known for high mutation rates caused by lack of protective histones, inefficient dna repair systems, and continuous exposure to mutagenic effects of oxygen radicals. alterations in the non-coding displacement (d) loop of mitochondrial dna are present in many cancers. it has been suggested that the extent of mitochondrial dna mutations might be useful in the prognosis of cancer outcome and/or the response to certain therapies. in order to investigate whether a high in ... | 2005 | 16359547 |
| germline genetic polymorphisms of cyp1a1, gstm1 and gstt1 genes in indian cervical cancer: associations with tumor progression, age and human papillomavirus infection. | host genetic factors may play a role in human papillomavirus (hpv)-associated tumorigenesis, although the issue continues to be a focus of much debate. biotransformation is critical in carcinogenic activity of numerous environmental carcinogens. it is therefore possible that polymorphisms of genes producing functional changes in xenobiotic metabolizing enzymes may be susceptible factors in cervical carcinogenesis. this study looked into possible relationships among these factors. | 2006 | 16360200 |
| lipoid proteinosis: an unusual presentation with verruca vulgaris. | lipoid proteinosis (lp) is a very rare, autosomally recessive, inherited disease. it is also known as urbach wiethe disease or hyalinosis cutis et mucosa. lp usually runs a benign, stable, and slowly progressing course. we present one lp case with additional unusual dermatologic involvement; verruca vulgaris was superimposed on papular lesions of lp. to our knowledge, this case is the first and only one described in the literature. in this presentation, we discuss the possibility of predispositi ... | 2005 | 16361720 |
| synthetic double-stranded rna poly(i:c) as a potent peptide vaccine adjuvant: therapeutic activity against human cervical cancer in a rodent model. | due to the inherent lack of immunogenicity of peptides, it is generally recognized that the strong inflammatory signals that are required to elicit specific responses against peptide-based therapeutic tumor vaccines may not be provided by the standard/conventional vaccine adjuvants. in this study, we have demonstrated dsrna in the form of synthetic pi:c as a potent adjuvant to enhance the specific anti-tumor immune responses against a peptide-based vaccine. when complexed with an mhc i-restricte ... | 2006 | 16362407 |
| cidofovir incorporation into human keratinocytes with episomal hpv 16 results in nonselective cytotoxicity. | recurrent respiratory papillomatosis (rrp) is caused by human papillomavirus (hpv). surgical excision is the mainstay of treatment; however, medical therapy including cidofovir, a cytosine analog, has been investigated. human papillomavirus does not encode a viral dna polymerase, which is the known target of cidofovir in cytomegalovirus infections. | 2005 | 16363057 |
| new viral vaccines. | vaccination is the most effective medical intervention against diseases caused by human viral pathogens. viral vaccines prevent or modify the severity of illness in the individual and interrupt or reduce the transmission of the pathogens to other susceptible people. through these mechanisms, vaccines against smallpox, polio, measles and hepatitis b have had an enormous impact on world health over the last 50 years. advances in basic virology and understanding of human immunity promise more progr ... | 2006 | 16364754 |
| new and surprising insights into pathogenesis of multicentric squamous cancers in the female lower genital tract. | 2005 | 16368936 | |
| dna vaccine against hamster oral papillomavirus-associated oral cancer. | previously we developed a carcinogenesis model involving the combination of 9,10-dimethyl-1,2-benzanthracene (dmba) application with physical wounding of hamster lingual mucosa. the presence of a novel hamster oral papillomavirus (hopv) was demonstrated and its genome sequenced. in the present study, this hopv hamster model was used to test whether vaccination with the l1 gene could prevent the development of oral carcinoma. dna plasmids encoding the l1 gene or the vector alone were injected int ... | 2005 | 16372582 |
| human papillomavirus and anal intraepithelial neoplasia. | a review of recent developments in the understanding of the natural history of anal squamous carcinoma arising from areas of high-grade anal intraepithelial neoplasia. | 2006 | 16374220 |
| who should be vaccinated against human papillomavirus? | 2006 | 16377523 | |
| inflation of sensitivity of cervical cancer screening tests secondary to correlated error in colposcopy. | to determine whether the sensitivity of screening tests that miss small cervical intraepithelial neoplasia (cin) 2 or worse (e.g., acetic acid-aided visual inspection) is inflated when the criterion standard (colposcopic-directed biopsy) misses the same small cin 2 or worse. | 2006 | 16378026 |
| refining the management of low-grade cervical abnormalities in the uk national health service and defining the potential for human papillomavirus testing: a commentary on emerging evidence. | 2006 | 16378029 | |
| the occurrence of genital types of human papillomavirus in normal pregnancy and in pregnant women with pregestational insulin dependent diabetes mellitus. | the aim of the study was to evaluate the prevalence of human papillomavirus, both types of a low oncogenic risk (hpv 6, 11) and a type carrying a high oncogenic risk (hpv 16) in the genital tract of the pregnant patients, their venous blood, the cord blood and the oral cavities of the neonates. normal pregnant women and pregnant women with insulin dependent diabetes mellitus (iddm) diagnosed before pregnancy were included in the study. | 2005 | 16380676 |
| the e7 proteins of low- and high-risk human papillomaviruses share the ability to target the prb family member p130 for degradation. | high-risk human papillomaviruses (hpvs) (e.g., hpv-16) cause anogenital and head and neck cancers, and low-risk hpvs (e.g., hpv-6) cause benign hyperproliferative disease. the e7 protein of hpv-16 binds all retinoblastoma tumor suppressor protein (prb) family members with higher affinity than hpv-6e7. the hpv-16 e7 protein has been reported to target prb family members for degradation and to immortalize cells. here we tested the hypothesis that the low-risk e7 protein has an intrinsic ability to ... | 2006 | 16381817 |
| chemoprevention of cervical cancer. | the greatest potential for the chemoprevention of cervical cancer is in women with human papillomavirus (hpv) infection, an abnormal screening test or a non-invasive neoplastic lesion. potential chemopreventive agents include micronutrients, antiviral agents and immune modifiers. randomised controlled clinical trials have generally been small and the results have not been encouraging. beneficial effects on neoplasia have been shown for a couple of agents that have since been abandoned due to adv ... | 2006 | 16386963 |
| interstitial cystitis and infectious agents. | interstitial cystitis (ic) is a syndrome consisting of severe refractory bladder symptoms of unknown etiology. the disease tends to affect caucasian women with a mean age of 40 years, with 25% of patients under the age of 30. few population based epidemiological studies of ic have been performed. we analyzed a case of interstitial cystitis in a 42-year-old non-smoker woman. in two biopsy samples the presence of viral dna of human polyomavirus bk (bkv), human herpes virus type 1 and type 2 (hhv- ... | 2005 | 16388730 |
| [study on the relationship between genesis and development of cervical cancer and the infection of human papillomavirus type 16/18, human herpesvirus ii and cytomegalovirus]. | to investigate the correlation between genesis and the development of cervical cancer and infection of human papillomavirus (hpv) type 16/18, human herpesvirus ii (hsv- ii) and cytomegalovirus(cmv). | 2005 | 16390015 |
| clinical significance of immunocytochemistry for pik3ca as a carcinogenesis-related marker on liquid-based cytology in cervical intraepithelial neoplasia. | the catalytic subunit alpha of phosphatidylinositol 3-kinase (pik3ca) has been expected to play a role as an important oncogene in uterine cervical carcinoma, whose expression in non-invasive lesions has received considerable attention. we investigated the potential of pik3ca as a carcinogenesis-related marker for early intraepithelial lesion of the uterine cervix in cytology samples. seventy-four cases with abnormal cytology suggesting the existence of cervical intraepithelial neoplasia (cin) l ... | 2006 | 16391859 |
| efficacy of human papillomavirus-16 vaccine to prevent cervical intraepithelial neoplasia: a randomized controlled trial. | human papillomavirus (hpv) virus-like particle (vlp) vaccines have demonstrated effectiveness in preventing persistent hpv infections. whether protection lasts longer than 18 months and, thus, impacts rates of cervical intraepithelial neoplasia (cin) 2-3 has not yet been established. we present results from an hpv16 l1 vlp vaccine trial through 48 months. | 2006 | 16394035 |
| hpv and p53 expression in epithelial ovarian carcinoma. | human papillomavirus is the causal factor for cervical cancer. however, the role of hpv infection in ovarian cancer is unclear. this study aimed to determine the presence of human papillomavirus (hpv) in ovarian cancer tissues along with the expression of tumor suppressor gene p53. we also investigated any possible association of hpv with p53 gene mutations in ovarian carcinoma. | 2005 | 16398227 |
| human papillomavirus (hpv) typing in relation to ras oncogene mrna expression in hpv-associated human squamous cervical neoplasia. | human papillomavirus (hpv) has been identified as the principal etiologic agent for cervical cancer and its precursors. different hpv types have been associated with different oncogenic potential. the purpose of this study was to evaluate the relationship between specific hpv type infection and expression pattern of the ras family oncogenes in different grades of hpv-associated human cervical neoplasia. | 2005 | 16398408 |
| ian frazer: finding a vaccine for human papillomavirus. | 2006 | 16399139 | |
| lifetime effects, costs, and cost effectiveness of testing for human papillomavirus to manage low grade cytological abnormalities: results of the nhs pilot studies. | to predict the incremental lifetime effects, costs, and cost effectiveness of using human papillomavirus testing to triage women with borderline or mildly dyskaryotic cervical smear results for immediate colposcopy. | 2006 | 16399769 |
| sexually transmitted infections, blood-borne viruses and risk behaviour in an australian senior high school population--the shlirp study. | to determine the feasibility and acceptability of screening for sexually transmitted infections and blood-borne viruses and to study the profile of sexual activity and other risk behaviours in a senior high school population. | 2005 | 16402670 |
| hpv information needs. | this chapter discusses the importance of providing women with information about human papillomavirus and its role as the causative agent in the development of cervical cancer. studies assessing current levels of knowledge among the general public are reviewed. what women need to know will be covered, followed by possible strategies for implementation. there are no randomised controlled trials to review regarding information for women about human papillomavirus but a number of well-conducted qual ... | 2006 | 16403488 |
| human papilloma virus and breast cancer. | 2006 | 16404422 | |
| suppression of non-specific binding in serological luminex assays. | bead-based suspension array technology (xmap, luminex corp.) permits the simultaneous analysis of antibodies with specificities for up to 100 different antigens in a single reaction and the high through-put screening of up to 1,000 sera per day. therefore, this technology is becoming more and more popular for serological analyses, replacing elisa techniques at least for epidemiological purposes. however, a major intrinsic problem of luminex technology is that human sera may contain antibodies th ... | 2006 | 16406059 |
| when to test women for human papillomavirus. | 2006 | 16410556 | |
| human papillomavirus infection in rio de janeiro, brazil: a retrospective study. | there is considerable data to support a central role for human papillomavirus (hpv) in the etiology of cervical cancer. more than a 100 hpv types have been described, and 40 have been isolated from benign and malignant genital lesions. consequently, there is strong motivation to evaluate hpv testing for cervical cancer screening. few studies concerning the natural history of hpv infection have been conducted in the state of rio de janeiro. we determined the prevalence of hpv types in female geni ... | 2006 | 16410891 |
| role of p16(ink4a) expression in identifying cin2 or more severe lesions among hpv-positive patients referred for colposcopy after abnormal cytology. | p16 is strongly overexpressed in dysplastic cervical cells because of the transforming activity of the e7 oncogene of all high-risk human papillomavirus (hr-hpv) types and may be easily revealed by immunochemistry: p16 may, therefore, be considered a surrogate marker for the activated oncogene expression of hr-hpv in dysplastic cervical cells. | 2006 | 16411180 |
| chaperone holdase activity of human papillomavirus e7 oncoprotein. | e7 oncoprotein is the major transforming activity in human papillomavirus and shares sequence and functional properties with adenovirus e1a and sv40 t-antigen, in particular by targeting the prb tumor suppressor. hpv 16 e7 forms spherical oligomers that display chaperone activity in thermal denaturation and chemical refolding assays of two model polypeptide substrates, citrate synthase and luciferase, and it does so at substoichiometric concentrations. we show that the e7 chaperone can stably bi ... | 2006 | 16411741 |
| efficiency of hpv 16 l1/e7 dna immunization: influence of cellular localization and capsid assembly. | infections by human papillomaviruses (hpv) are the major cause of uterine cancer in women worldwide. aiming to develop a combined prophylactic and therapeutic vaccine we have previously demonstrated immunogenicity of chimeric virus-like particles consisting of a c-terminally truncated hpv 16 l1 capsid protein fused to an e7 portion. here we show that genetic vaccination with a corresponding dna was inefficient in the induction of a l1-specific prophylactic immune response. dna immunization with ... | 2006 | 16414157 |
| papillomavirus e1 protein binds to and stimulates human topoisomerase i. | the papillomavirus (pv) e1 helicase plays a direct role in recruiting cellular dna replication factors, such as replication protein a or polymerase alpha-primase, to replicate pv genomes. here, e1 is shown to bind to human topoisomerase i and stimulate its relaxation activity up to sevenfold. the interaction between e1 and topoisomerase i was mapped to the e1 dna binding domain and c terminus. these findings imply a mechanism for the recruitment of topoisomerase i to pv dna replication forks and ... | 2006 | 16415033 |
| treatment for simple plantar verrucae: monochloroacetic acid and 10% formaldehyde versus 10% formaldehyde alone. | verrucae are small, benign, highly vascular epithelial neoplasms that occur singly or in a multiple presentation. plantar verrucae are usually caused by infection with human papillomavirus types 1, 2, and 4. a clinical trial was conducted to assess the safety and efficacy of monochloroacetic acid and 10% formaldehyde versus 10% formaldehyde alone in the treatment of simple plantar verrucae. of 57 patients enrolled in the study, 26 were in the monochloroacetic acid and 10% formaldehyde group and ... | 2006 | 16415283 |
| [cervical glandular intraepithelial neoplasia topography and the risk of conisation]. | the frequency of endocervical adenocarcinoma is increasing in comparison with squamous cell carcinoma and it presents a very difficult diagnostic and therapeutic problem. | 2005 | 16417091 |
| prophylactic vaccines for human papillomavirus: a bright future for cervical cancer prevention. | 2005 | 16417690 | |
| cost-effectiveness analysis based on the atypical squamous cells of undetermined significance/low-grade squamous intraepithelial lesion triage study (alts). | the alts (atypical squamous cells of undetermined significance [ascus] and low-grade squamous intraepithelial lesion [lsil] triage study) suggests that, for women diagnosed with ascus, human papillomavirus (hpv) dna testing followed by referral to colposcopy of only those women with oncogenic hpv (i.e., hpv dna testing) is as effective at detecting cervical intraepithelial neoplasia (cin) 3 or cancer (cin3+) as referring all women with ascus for immediate colposcopy. we conducted a cost-effectiv ... | 2006 | 16418511 |
| a case-control study of human papillomavirus and cervical squamous intraepithelial lesions (sil) in harris county, texas: differences among racial/ethnic groups. | 2005 | 16419209 | |
| prospects for therapeutic hpv vaccines. | 2005 | 16419216 | |
| distribution of human papillomavirus types in thinprep papanicolaou tests classified according to the bethesda 2001 terminology and correlations with patient age and biopsy outcomes. | a survey of the distribution of human papillomavirus (hpv) types across the spectrum of cervical cytologic categories defined by the bethesda 2001 guidelines was conducted with the objective of examining how hpv detection by polymerase chain reaction (pcr) analysis may benefit the management of patients who have abnormal papanicolaou (pap) test results. | 2006 | 16421920 |
| high-risk human papilloma virus infection decreases the frequency of dendritic langerhans' cells in the human female genital tract. | dendritic cells (dc) are often arranged in planar layers in tissues with high antigenic exposure, such as skin and mucosae. providing an en face view, this arrangement optimizes in situ analysis regarding morphology (even of individual dendrites), topographic distribution (regular/clustered) and quantification. the few reports on human genital dc usually utilize single markers and conventional sections, restricting immunolabelling only to cell parts sectioned by the cut. to better assess dc in s ... | 2006 | 16423058 |
| age-related changes of the cervix influence human papillomavirus type distribution. | approximately 15 human papillomavirus (hpv) types cause virtually all cervical cancer whereas other hpv types are unrelated to cancer. we were interested in whether some noncarcinogenic types differ from carcinogenic in their affinity for the cervical transformation zone, where nearly all hpv-induced cancers occur. to examine this possibility, we tested cervical specimens from 8,374 women without cervical precancer and cancer participating in a population-based study in guanacaste for >40 hpv ty ... | 2006 | 16424061 |
| upregulation of mortalin/mthsp70/grp75 contributes to human carcinogenesis. | mortalin, also known as mthsp70/grp75/pbp74, interacts with the tumor suppressor protein p53 and inactivates its transcriptional activation and apoptotic functions. here, we examined the level of mortalin expression in a large variety of tumor tissues, tumor-derived and in vitro immortalized human cells. it was elevated in many human tumors, and in all of the tumor-derived and in vitro immortalized cells. in human embryonic fibroblasts immortalized with an expression plasmid for htert, the telom ... | 2006 | 16425258 |
| quantitative determination of human telomerase reverse transcriptase messenger rna expression in premalignant cervical lesions and correlation with human papillomavirus load. | expression of human telomerase reverse transcriptase (htert) messenger rna (mrna) and human papillomavirus (hpv)-16 load were quantified using real-time polymerase chain reaction and correlated with cytological findings and the presence of hpv infection in cervical specimens. human telomerase reverse transcriptase mrna expression was evaluated in 15 (20.5%) of 73 specimens of atypical squamous epithelial cells of undetermined significance, in 62 (39.7%) of 156 low-grade squamous intraepithelial ... | 2006 | 16426912 |
| distribution of hpv16 and 18 intratypic variants in normal cytology, intraepithelial lesions, and cervical cancer in a mexican population. | several intratype variants of hpv16 and 18 have been identified. these variants are associated with populations from different geographic regions, and show a differential distribution among the severity of the cervical lesion, most likely due to different pathogenic potential. the objective of this study was to investigate the variant distribution of hpv16 and 18 in a mexican population and its association with the severity of the cervical lesion and the histological lineage of cervical cancer. | 2006 | 16427686 |
| cervical cancer and hpv. | 2005 | 16430069 | |
| effects of human papillomavirus type 16 oncoproteins on survivin gene expression. | survivin has recently been identified as a novel member of the inhibitor of apoptosis (iap) gene family. the product of this gene not only suppresses apoptosis but also controls cell division. survivin is undetectable in most terminally differentiated normal tissues but is expressed in embryonic and fetal organs and is present in most malignant tumours. human papillomaviruses (hpv) are thought to play an important role in the development of cervical cancer. by interfering in the cell cycle, the ... | 2006 | 16432013 |
| measures of sexual partnerships: lengths, gaps, overlaps, and sexually transmitted infection. | the length of time between partnerships ("gap") is an important determinant of the overall transmission system of sexually transmitted infections. we describe the distributions of gaps, lengths, and overlaps among participants in a random-digit dialing survey conducted among 1194 seattle residents during 2003-2004. | 2006 | 16434884 |
| increased expression of 70 kd heat shock protein in cultured primary human keratinocytes induced by human papillomavirus 16 e6/e7 gene. | heat shock protein 70 (hsp70) is expressed highly in epithelial tumours associated closely with human papillomavirus 16 (hpv16) infections. however, evidence about the direct relationship between hsp70 expression and hpvs infections are still lacking. in the present study, we examined the expression of hsp70 in keratinocytes introduced with hpv16 e6/e7 oncogenes. | 2005 | 16438903 |
| intravenous cidofovir treatment for recalcitrant warts in the setting of a patient with myelodysplastic syndrome. | cidofovir is an acyclic nucleoside phosphonate with broad-spectrum activity against dna viruses, including human papilloma virus (hpv). however, data on the efficacy of cidofovir in an immunosuppressive setting remain contradictory. we report for the first time on the promotion of the healing of recalcitrant warts in a patient with myelodysplastic syndrome with intravenous cidofovir treatment. | 2006 | 16441633 |
| development of anticancer gene vaccine interact with human papillomavirus oncoprotein inhibition. | adeno-associated virus (aav) rep 78 protein is known to inhibit the promoter site of several oncogenes and viral genes, including the human papillomavirus (hpv) type 16 e6 transforming genes. the biochemical studies of rep 78 have been reported, but the effects of rep 78 gene-mediated inhibition of hpv 16 e6 promoter activity on the various human cervical carcinoma cells have not been characterized. pegfp-n1 vector, cloned by aav-mediated rep 78, is transfected into cervical carcinoma cells. tra ... | 2006 | 16445644 |
| human papillomavirus in melanoma. | 2006 | 16445807 | |
| recombinant alphaviruses as vectors for anti-tumour and anti-microbial immunotherapy. | vectors derived from alphaviruses are gaining interest for their high transfection potency and strong immunogenicity. | 2006 | 16448844 |
| comparison of physician- and self-collected genital specimens for detection of human papillomavirus in men. | there is currently no consensus regarding the most appropriate methods of sampling for the detection of genital human papillomavirus (hpv) in men. we employed a recently developed collection method involving abrasion and moistened swabbing of the genital skin surface for the detection of hpv in a cohort of 136 university-affiliated males in hawaii. genital specimens collected by physicians using this method were compared with self-collected specimens from the same individuals obtained 24 h later ... | 2006 | 16455906 |
| regression of papilloma high-grade lesions (cin 2 and cin 3) is stimulated by therapeutic vaccination with mva e2 recombinant vaccine. | human papillomavirus (hpv) is the etiologic agent for cervical cancer. in mexico, a women dies every 2 h, and since 1990 the statistics have shown that the numbers of deaths are increasing. we conducted a phase ii clinical trial to evaluate the potential use of the mva e2 recombinant vaccinia virus in treating high-grade lesions (cin 2 and cin 3) associated with oncogenic papillomavirus. fifty-four female patients with high degree lesions were treated either with an mva e2 therapeutic vaccine or ... | 2006 | 16456551 |
| elevation of cyclin b1, active cdc2, and hur in cervical neoplasia with human papillomavirus type 18 infection. | over 30 cervical epitheliotrophic hpv types may lead to altered biological functions that affect the clinical outcome of hpv infection. in order to determine the regulatory mechanism and effect of different hpv subtypes, we performed functional assays on cdc2, cyclinb1 and hur in human uterine cervical samples. after confirming 22 hpv types among 95 cervical swabs, 10 cervical tissues, and seven established cell lines using a dna chip, we evaluated the functional activities of g2 molecules assay ... | 2006 | 16458113 |
| potential applications of oral brush cytology with liquid-based technology: results from a cohort of normal oral mucosa. | fifty healthy volunteers were studied to assess the potential applications of oral brush sampling using liquid-based cytology. three specimens from the buccal mucosa and lateral border of tongue were collected from each subject by using cervical brushes and brooms. the brush was immersed in a preservative fluid. the sample in the preservative fluid was processed according to the manufacturer's directions (surepath, uk). slides were stained by the papanicolaou method and assessed for squamous cel ... | 2006 | 16458571 |
| a survey of female nursing students' knowledge, health beliefs, perceptions of risk, and risk behaviors regarding human papillomavirus and cervical cancer. | the purpose of the study was to assess knowledge of, perceived susceptibility to, perceived seriousness of, and risk behaviors regarding human papillomavirus (hpv) and cervical cancer among female nursing students. | 2006 | 16460412 |
| advances in prevention of cervical cancer and other human papillomavirus-related diseases. | 2006 | 16462611 | |
| turning cervix cancer into a "rare disease": is society prepared to do what is required to make this a reality? | 2006 | 16464396 | |
| [inhibition of hpv16 e6 oncogene in cervical cancer by rna interference]. | the efficiency of hpv16 e6 gene silenced by rna interference in vitro and in vivo was assessed. | 2006 | 16468632 |
| effect of hiv infection on atypical squamous cells of undetermined significance. | detection of atypical squamous cells of undetermined significance (ascus) is a cervical cytologic finding that is suggestive but not definitive of squamous intraepithelial lesions (sils). | 2006 | 16477565 |
| why do we go on screening? | as healthcare rules are becoming better organized and as vaccination against human papillomavirus (hpv) infections is arising, the conditions of cytological screening for cervical carcinoma may have to be modified in the near future. after reminding the quality control constraints of the pap test and the technical and epidemiological conditions for the hpv detection as a precursor of cervical carcinoma, the present paper wishes to discuss the possible role of vaccine on the screening strategy. | 2005 | 16477875 |
| oligonucleotide microarray with rd-pcr labeling technique for detection and typing of human papillomavirus. | currently, screening for high-risk human papillomavirus (hpv) infection remains an important health concern throughout the world, because of the close association between certain types of hpv and cervical cancer. in this study, we explore the possibility of using approximately 70mer oligonucleotide microarray for detection and genotyping of hpv. the approximately 70mer type-specific oligonucleotide probes of four different types hpv were designed by using biological software array designer 2.0, ... | 2006 | 16479359 |
| polyhexamethylene biguanide for treatment of external genital warts: a prospective, double-blind, randomized study. | genital human papillomavirus infection is one of the most common sexually transmitted diseases. polyhexamethylene biguanide is a new agent, that has been demonstrated to have potent in vivo antiviral effects in animal and in human models. the present prospective, double-blind, randomized, placebo (vehicle-controlled) trial evaluated the efficacy and safety of daily patient-applied polyhexamethylene biguanide for up to 16-weeks for the treatment of external genital warts. wart recurrence was inve ... | 2005 | 16479742 |
| therapeutic potential of an adenovirus expressing p73 beta, a p53 homologue, against human papilloma virus positive cervical cancer in vitro and in vivo. | human papilloma virus (hpv) infection is the most important risk factor for cervical cancer development. p53 based gene therapy is not suitable for cervical cancer because hpv oncoprotein e6 inactivates p53 protein by targeting it for ubiquitin mediated degradation. here we evaluated the efficiency of ad-p73, a replication deficient adenovirus expressing p73beta a p53 homologue, to inhibit the growth of hpv positive cervical cancer cells in vitro using tissue culture system and in vivo using hum ... | 2006 | 16481734 |
| hpv dna patterns and disease implications in the follow-up of patients treated for hpv16 high-grade carcinoma in situ. | twenty-five patients with high-grade cervical lesions associated with hpv16 infection were studied at the time of surgical treatment and followed up after conization. before surgical treatment, the following parameters were analyzed: (1) physical status of hpv16 dna, (2) viral load, (3) cytological presentation confirmed by histological diagnosis, and (4) colposcopy. at the time of conization, (5) margin presentation, and (6) cone biopsy were evaluated. at each stage of the follow-up (7) physica ... | 2006 | 16482538 |
| involvement of a cellular ubiquitin-protein ligase e6ap in the ubiquitin-mediated degradation of extensive substrates of high-risk human papillomavirus e6. | human scribble (hscrib), which was identified as substrate of human papillomavirus (hpv) e6 for ubiquitin-mediated degradation dependent on ubiquitin-protein ligase e6ap, is a human homolog of drosophila neoplastic tumor suppressor scribble, in which mutation causes loss of polarity and overgrowth of epithelia. drosophila discs large (dlg) is one of neoplastic tumor suppressors, which genetically links to scribble. e6 also targets human dlg (hdlg) for ubiquitin-mediated degradation. ubiquitin-pr ... | 2006 | 16482544 |