Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| development of carboxymethyl cellulose-based hydrogel and nanosilver composite as antimicrobial agents for uti pathogens. | silver nanoparticles (agnps) containing hydrogel composite were first synthesized by preparing a new hydrogel from carboxymethyl cellulose (cmc), polyvinyl alcohol (pva), and the cross-linker ethylene glycol diglycidyl ether (egde), followed by the incorporation of agnps by microwave radiation. the resulting neat hydrogels and agnps-hydrogel composites were characterized using spectral, thermal, microscopic analysis and x-ray diffraction (xrd) analyses. the sem and tem results demonstrated that ... | 2016 | 26794757 |
| characterisation of inca/c2 plasmids carrying an in416-like integron with the blavim-19 gene from klebsiella pneumoniae st383 of greek origin. | the complete nucleotide sequences of three multidrug resistance (mdr) inca/c-like plasmids from enterobacteriaceae isolates carrying the vim-type carbapenemase-encoding integrons in4863 (blavim-19-aaca7-dfra1-δaada1-smr2) or in4873 (blavim-1-aaca7-dfra1-δaada1-smr2) were determined, which are the first in416-like elements identified in greece. plasmids pkp-gr642 and pkp-gr8143 were from klebsiella pneumoniae st383 isolates, whereas plasmid pecl-gr4873 was from an enterobacter cloacae st88 isolat ... | 2016 | 26795022 |
| draft genome sequences of two multidrug-resistant extended-spectrum-β-lactamase-producing klebsiella pneumoniae strains causing bloodstream infections. | multidrug-resistant (mdr) klebsiella pneumoniae has become a major contributor to nosocomial bloodstream infections. here, we report the draft genome sequences of two mdr extended-spectrum-β-lactamase-producing strains causing bloodstream infections. these sequenced genomes display a wide-spectrum virulence arsenal and will help us understand the genomic basis of k. pneumoniae virulence. | 2016 | 26798092 |
| acute conditions caused by infectious aortitis. | infection of the aorta is rare but potentially very dangerous. under normal circumstances the aorta is very resistant to infections. following some afflictions, the infection can pass to the aorta from blood or the surrounding tissues. the authors present their 5-year experience with therapy of various types of infections of the abdominal aorta. | 2014 | 26798723 |
| spinal epidural abscess following glossectomy and neck dissection: a case report. | spinal epidural abscess is an uncommon but potentially life threatening entity that rarely occurs after otolaryngology procedures. | 2016 | 26799413 |
| in vitro antimicrobial and anti-proliferative activities of plant extracts from spathodea campanulata, ficus bubu, and carica papaya. | african medicinal plants represent a prominent source of new active substances. in this context, three plants were selected for biological investigations based on their traditional uses. | 2016 | 26799575 |
| outbreak of ndm-1-producing klebsiella pneumoniae st76 and st37 isolates in neonates. | the purpose of this study was to investigate the epidemiological characteristics of carbapenem-resistant klebsiella pneumoniae (crkp) in shanghai children's hospital in china. twenty-two non-duplicate crkp strains were collected from pediatric patients between march and june in 2014. antimicrobial susceptibility testing was conducted by the agar dilution method. beta-lactamases were characterized by polymerase chain reaction (pcr) and dna sequencing. the transferability of bla ndm-1 was investig ... | 2016 | 26803822 |
| diversity of bacterial communities on four frequently used surfaces in a large brazilian teaching hospital. | frequently used hand-touch surfaces in hospital settings have been implicated as a vehicle of microbial transmission. in this study, we aimed to investigate the overall bacterial population on four frequently used surfaces using a culture-independent illumina massively parallel sequencing approach of the 16s rrna genes. surface samples were collected from four sites, namely elevator buttons (eb), bank machine keyboard buttons (bmkb), restroom surfaces, and the employee biometric time clock syste ... | 2016 | 26805866 |
| complete genome sequence of klebsiella pneumoniae j1, a protein-based microbial flocculant-producing bacterium. | klebsiella pneumoniae j1 is a gram-negative strain, which belongs to a protein-based microbial flocculant-producing bacterium. however, little genetic information is known about this species. here we carried out a whole-genome sequence analysis of this strain and report the complete genome sequence of this organism and its genetic basis for carbohydrate metabolism, capsule biosynthesis and transport system. | 2016 | 26806487 |
| spectrum of microbial diseases and resistance patterns at a private teaching hospital in kenya: implications for clinical practice. | accurate local prevalence of microbial diseases and microbial resistance data are vital for optimal treatment of patients. however, there are few reports of these data from developing countries, especially from sub-saharan africa. the status of aga khan university hospital nairobi as an internationally accredited hospital and a laboratory with an electronic medical record system has made it possible to analyze local prevalence and antimicrobial susceptibility data and compare it with other publi ... | 2016 | 26807811 |
| redefining healthy urine: a cross-sectional exploratory metagenomic study of people with and without bladder dysfunction. | we used the pathoscope platform to perform species level analyses of publicly available, 16s rrna pyrosequenced, asymptomatic urine data to determine relationships between microbiomes, and clinical and functional phenotypes. | 2016 | 26807926 |
| carbapenem-resistant klebsiella pneumoniae: results of a laboratory surveillance program in an italian general hospital (august 2014-january 2015) : surveillance of carbapenem-resistant klebsiella pneumoniae. | in this study we report the analysis of 131 klebsiella pneumoniae (k. pneumoniae) clinical isolates from patients hospitalized in various wards, of perugia general hospital, from august 2014 to january 2015. forty two isolates (32.1 %), were resistant to at least one carbapenem antibiotic and, among these isolates, 14 (33.3 %) exhibited resistance to colistin. all isolates were carbapenemases producers and 41 (97.6 %) harboured the bla kpc gene. carbapenem-resistant k. pneumoniae isolates (crkps ... | 2016 | 26810235 |
| klebsiella pneumoniae is able to trigger epithelial-mesenchymal transition process in cultured airway epithelial cells. | the ability of some bacterial pathogens to activate epithelial-mesenchymal transition normally is a consequence of the persistence of a local chronic inflammatory response or depends on a direct interaction of the pathogens with the host epithelial cells. in this study we monitored the abilities of the k. pneumoniae to activate the expression of genes related to emt-like processes and the occurrence of phenotypic changes in airway epithelial cells during the early steps of cell infection. we des ... | 2016 | 26812644 |
| pharmaco-phylogenetic investigation of methyl gallate isolated from acacia nilotica (l.) delile and its cytotoxic effect on nih3t3 mouse fibroblast. | present exploration deals with the therapeutic perspective of methyl gallate isolated from the leaf extract of acacia nilotica (l.) delile in contrast to food-borne bacterial pathogen's viz., escherichia coli, klebsiella pneumoniae, salmonella typhimurium, pseudomonas aeruginosa and staphylococcus aureus with their evolutionary succession. the extract was subjected to phytochemical analysis and isolated compound was identified as methyl gallate using uv-vis, ir and nmr spectra. it was found most ... | 2016 | 26813302 |
| virulence and antimicrobial resistance of klebsiella pneumoniae isolated from passerine and psittacine birds. | klebsiella pneumoniae is considered one of the most important gram-negative opportunistic pathogens. the contact between humans and birds poses health risks to both. the aim of this study was to investigate the resistance and virulence of k. pneumoniae isolates from psittacines and passerines, seized from illegal trade in brazil. we analysed 32 strains isolated from birds of the orders psittaciformes and passeriformes by polymerase chain reaction (pcr) for virulence factor genes. antibiotic resi ... | 2016 | 26813537 |
| kisameet clay exhibits potent antibacterial activity against the eskape pathogens. | the eskape (enterococcus faecium, staphylococcus aureus, klebsiella pneumoniae, acinetobacter baumannii, pseudomonas aeruginosa, and enterobacter species) pathogens cause an increasing number of nosocomial infections worldwide since they escape the inhibitory effect of the available antibiotics and the immune response. here, we report the broad-spectrum and potent antibacterial activity of kisameet clay, a natural clay mineral from british columbia, canada, against a group of multidrug-resistant ... | 2016 | 26814180 |
| pathway engineering of propionibacterium jensenii for improved production of propionic acid. | propionic acid (pa) is an important chemical building block widely used in the food, pharmaceutical, and chemical industries. in our previous study, a shuttle vector was developed as a useful tool for engineering propionibacterium jensenii, and two key enzymes-glycerol dehydrogenase and malate dehydrogenase-were overexpressed to improve pa titer. here, we aimed to improve pa production further via the pathway engineering of p. jensenii. first, the phosphoenolpyruvate carboxylase gene (ppc) from ... | 2016 | 26814976 |
| draft genome of multidrug-resistant klebsiella pneumoniae 223/14 carrying kpc-6, isolated from a general hospital in malaysia. | we performed whole genome sequencing on a clinical multidrug-resistant klebsiella pneumoniae strain 223/14. investigation into its draft genome revealed the presence of kpc-6 variant, suggesting carbapenemase is present in this isolate. we found a plasmid-borne kpc gene (882 bp) inserted between two transposase genes in the genome of k. pneumoniae 223/14. | 2015 | 26816553 |
| klebsiella pneumoniae thyroid abscess complicated with esophagitis in a woman with newly diagnosed diabetes mellitus: a case report. | the thyroid is resistant to infection because of the anatomical and physiological characteristics of the gland, and even though diabetes can induce a compromised immune system, thyroid infection disease is scarcely encountered in diabetic patients. thyroid abscess formation in an asymptomatic diabetic patient is an even rarer entity. we present a case of a previously asymptomatic diabetic patient showing clinical symptoms of painful swelling in the anterior neck followed by progressing dysphagia ... | 2016 | 26816610 |
| intra-abdominal infections: prevalence and risk factors of esbls infections. | extended-spectrum beta-lactamases (esbls)-producing bacteria are increasing challenge in the treatment of both community acquired and nosocomial intra-abdominal infections (iais). investigating the characteristics ofpatients with the iais caused by esbl-producing bacteria and assessing the risk factors of esbls infection will lead to appropriate therapeutic management. | 2015 | 26817180 |
| detection and characterization of two ndm-1-producing klebsiella pneumoniae strains from bulgaria. | 2016 | 26817486 | |
| emergence of pan-resistance in kpc-2 carbapenemase-producing klebsiella pneumoniae in crete, greece: a close call. | kpc-2-producing klebsiella pneumoniae (kpc-kp) st258 has been rapidly expanding and is often associated with serious nosocomial infections. last-line antibiotics such as colistin and tigecycline often remain the only treatment option. we describe here the evolving genetic background of kpc-kp isolates in crete, greece. | 2016 | 26817488 |
| [antimicrobial resistance trends in pathogens isolated from nosocomial infections]. | the treatment of nosocomial infections is hindered by the increasing antimicrobial resistance pattern of germs that cause them. the objective was to assess trends in resistance of bacteria isolated from nosocomial infections. | 2017 | 26820196 |
| reutilization of green liquor chemicals for pretreatment of whole rice waste biomass and its application to 2,3-butanediol production. | the performance of green liquor pretreatment using na2co3 and na2so3 and its optimization for whole rice waste biomass (rwb) was investigated. incubation of na2co3-na2so3 at a 1:1 ratio (chemical charge 10%) for 12% rwb at 100°c for 6h resulted in maximum delignification (58.2%) with significant glucan yield (88%) and total sugar recovery (545mg/g of rwb) after enzymatic hydrolysis. recovery and reusability of the resulting chemical spent wash were evaluated to treat rwb along with its compatibi ... | 2016 | 26820921 |
| draft genome sequence of klebsiella pneumoniae kgm-imp216 harboring blactx-m-15, bladha-1, blatem-1b, blandm-1, blashv-28, and blaoxa-1, isolated from a patient in lebanon. | we present the draft genome of highly drug-resistant klebsiella pneumoniae kgm-imp216, isolated from a urine sample collected from a patient in lebanon. the draft genome sequence consisted of 77 contigs, including a combined 5,731,500 bases with 57% g+c content. | 2016 | 26823584 |
| complete genome sequence of kpc-producing klebsiella pneumoniae strain cav1193. | carbapenem resistance in klebsiella pneumoniae, frequently conferred by the blakpc gene, is a major public health threat. we sequenced a blakpc-containing strain of k. pneumoniae belonging to the emergent lineage st941, in order to better understand the evolution of blakpc within this species. | 2016 | 26823590 |
| burden of hospital acquired infections and antimicrobial use in vietnamese adult intensive care units. | vietnam is a lower middle-income country with no national surveillance system for hospital-acquired infections (hais). we assessed the prevalence of hospital-acquired infections and antimicrobial use in adult intensive care units (icus) across vietnam. | 2016 | 26824228 |
| γ-cyclodextrin capped silver nanoparticles for molecular recognition and enhancement of antibacterial activity of chloramphenicol. | computational studies were conducted to identify the favourable formation of the inclusion complex of chloramphenicol with cyclodextrins. the results of molecular docking and molecular dynamics predicted the strongest interaction of chloramphenicol with γ-cyclodextrin. further, the inclusion complex of chloramphenicol with γ-cyclodextrin was experimentally prepared and a phenomenon of inclusion was verified by using different characterization techniques such as thermogravimetric analysis, differ ... | 2016 | 26824520 |
| receptor for advanced glycation end products (rage) serves a protective role during klebsiella pneumoniae - induced pneumonia. | klebsiella species is the second most commonly isolated gram-negative organism in sepsis and a frequent causative pathogen in pneumonia. the receptor for advanced glycation end products (rage) is expressed on different cell types and plays a key role in diverse inflammatory responses. we here aimed to investigate the role of rage in the host response to klebsiella (k.) pneumoniae pneumonia and intransally inoculated rage gene deficient (rage-/-) and normal wild-type (wt) mice with k. pneumoniae. ... | 2016 | 26824892 |
| blandm-5-bearing incfii-type plasmids of klebsiella pneumoniae sequence type 147 transmitted by cross-border transfer of a patient. | the two plasmids extracted from klebsiella pneumoniae sequence type 147 (st147) isolates were analyzed. the first isolate was obtained from a patient transferred from united arab emirates to south korea. the second isolate was obtained from a korean patient and was suspected to be transmitted from the first patient. sequences of two plasmids were almost the same, and genetic structures, including blandm-5, of these plasmids were similar to plasmids of ndm-1-producing escherichia coli st131 isola ... | 2016 | 26824953 |
| colistin resistance caused by inactivation of the mgrb regulator is not associated with decreased virulence of sequence type 258 kpc carbapenemase-producing klebsiella pneumoniae. | using agalleria mellonellaanimal model, we compared the virulence of two sequence type 258 (st258) kpc-producingklebsiella pneumoniaestrains, which were representative of the two clades of this clonal lineage, with that of isogenic colistin-resistantmgrbmutants. with both strains, themgrbmutants did not exhibit modification in virulence. in theg. mellonellamodel, the clade 1 strain (capsular typecps-1[wzi29, producing kpc-2]) was significantly more virulent than the clade 2 strain (capsular type ... | 2016 | 26824959 |
| a retrospective analysis of the etiologic agents and antibiotic susceptibility pattern of uropathogens isolated in the jigme dorji wangchuck national referral hospital, thimphu, bhutan. | urinary tract infection is one of the major public health problems. specific population studies to understand the common etiologic agents and antibiotic susceptibility patterns are important to determine the empirical treatment of urinary tract infections. this is the first study in bhutan to analyze the etiologic agents and antibiotic susceptibility pattern of uropathogens isolated from patients visiting jigme dorji wangchuck national referral hospital with the ultimate goal of guiding empirica ... | 2016 | 26825932 |
| biological activities of some acacia spp. (fabaceae) against new clinical isolates identified by ribosomal rna gene-based phylogenetic analysis. | nowadays,most of the pathogenic bacteria become resistant to antibiotics. therefore,the pharmaceutical properties of the natural plant extracts have become of interest to researchers as alternative antimicrobial agents. in this study,antibacterial activities of extract gained from acacia etbaica, acacia laeta, acacia origena and acacia pycnantha have been evaluated against isolated pathogenic bacteria (strains mfm-01, mfm-10 and ah-09) using agar well diffusion methods.the bacterial strains were ... | 2016 | 26826814 |
| ethanolic extract of nigella sativa protects fe(ii) induced lipid peroxidation in rat's brain, kidney and liver homogenates. | the study describes the effect of ethanolic extract of nigella sativa against fe(ii) induced lipid peroxidation. basal and fe(ii) induced thiobarbituric acid reactive species (tbars) production was significantly inhibited by the ethanolic extract of nigella sativa at 25-200 μg/ml. our data revealed that the extract has high dpph radical scavenging activity at highest tested concentrations. the extract significantly chelated fe(ii) and scavenged hydroxyl (oh) radical at 25-200μg/ml concentration. ... | 2016 | 26826815 |
| in vitro antimicrobial activities of different solvent extracted samples from iris germinica. | antibacterial and antifungal activities of different solvents extracted samples of iris germinica were carried out through disc diffusion assay. for this purpose five different solvent extracts were prepared with two concentrations (1 and 2 mg disc(-1)) and their antimicrobial activity was tested using disc diffusion assay against eight pathogenic bacteria viz. staphylococcus aureus, b. subtilis, bacillus atrophaeus, escherichia coli, pseudomonas aeruginosa, erwinia carotovara, klebsiella pneumo ... | 2016 | 26826828 |
| report: sensitivity pattern of ceftriaxone against different clinical isolates. | emerging resistance against broad-spectrum antibiotics for standard empiric therapy is a global concern. ceftriaxone (broad spectrum, third generation cephalosporin) is widely used in tertiary care settings to treat severe bacterial infections usually non-responsive to other antibiotics. the aim of the study is to evaluate the current sensitivity pattern of ceftriaxone (30μg/disk) among different clinical isolates. for this purpose, three hundred clinical isolates including escherichia coli (25% ... | 2016 | 26826836 |
| design, synthesis and biological evaluation of monobactams as antibacterial agents against gram-negative bacteria. | a series of monobactam derivatives were prepared and evaluated for their antibacterial activities against susceptible and resistant gram-negative strains, taking aztreonam and bal30072 as the leads. six conjugates (12a-f) bearing pih-like siderophore moieties were created to enhance the bactericidal activities against gram-negative bacteria based on trojan horse strategy, and all of them displayed potencies against susceptible gram-negative strains with mic ≤ 8 μg/ml. sar revealed that the polar ... | 2016 | 26827160 |
| [rational aspects of bacteriophages use]. | analysis of existing aspects of bacteriophage use and study features of their lytic activity by using various techniques. | 2016 | 26829858 |
| blactx-m-15 carried by incf-type plasmids is the dominant esbl gene in escherichia coli and klebsiella pneumoniae at a hospital in ghana. | escherichia coli and klebsiella pneumoniae producing extended-spectrum β-lactamases (esbls) are among the most multidrug-resistant pathogens in hospitals and are spreading worldwide. horizontal gene transfer and spread of high-risk clones are involved in esbl dissemination. investigation of the resistance phenotypes of 101 consecutive clinical e. coli (n=58) and k. pneumoniae (n=43) isolated at the komfo anokye teaching hospital in ghana over 3 months revealed 63 (62%) with an esbl phenotype. al ... | 2016 | 26830052 |
| a pilot study of quantitative loop-mediated isothermal amplification-guided target therapies for hospital-acquired pneumonia. | it is important to achieve the definitive pathogen identification in hospital-acquired pneumonia (hap), but the traditional culture results always delay the target antibiotic therapy. we assessed the method called quantitative loop-mediated isothermal amplification (qlamp) as a new implement for steering of the antibiotic decision-making in hap. | 2016 | 26830989 |
| detection of klebsiella. pneumoniae infection with an antisense oligomer against its ribosomal rna. | previously, we demonstrated specific accumulation into bacteria of a 12-mer phosphorodiamidate morpholino (morf) oligomer complementary to a ribosomal rna (rrna) segment found in all bacteria using the universal probe called eub338 (eub). here, two morf oligomers eco and kpn with sequences specific to the rrna of escherichia coli (eco) and klebsiella pneumoniae (kpn) were investigated along with eub and control (noneub). | 2016 | 26832678 |
| concomitant and multiclonal dissemination of oxa-48-producing klebsiella pneumoniae in a spanish hospital. | 2016 | 26832748 | |
| characterization of st258 colistin-resistant, blakpc-producing klebsiella pneumoniae in a greek hospital. | the emergence of colistin resistance may further contribute to treatment failure of infection caused by multidrug-resistant (mdr) klebsiella pneumoniae. the colistin resistance rates were determined and colistin-resistant carbapenemase-producing k. pneumoniae (col-r cp-kp) were characterized over an 18-month period in a greek hospital. out of 135 carbapenemase producers, 19 isolates (14%) were categorized as resistant to colistin. phenotypic and molecular characterization of the col-r cp-kp isol ... | 2016 | 26840950 |
| bloodstream infections in pediatric patients at cancer institute, chennai. | there is paucity of data on the epidemiology of bloodstream infections in pediatric cancer patients from india. rationale use of antibiotics in febrile neutropenia is important for reducing morbidity and preventing the emergence of drug resistant bacteria. | 2016 | 26842144 |
| epidemiology of blood stream infections in pediatric patients at a tertiary care cancer centre. | blood stream infections (bsi) are among the most common causes of preventable deaths in children with cancer in a developing country. knowledge of its etiology as well as antibiotic sensitivity is essential not only for planning antimicrobial policy, but also the larger infection prevention and control measures. | 2016 | 26842152 |
| graphene-iodine nanocomposites: highly potent bacterial inhibitors that are bio-compatible with human cells. | graphene-composites, capable of inhibiting bacterial growth which is also bio-compatible with human cells have been highly sought after. here we report for the first time the preparation of new graphene-iodine nano-composites via electrostatic interactions between positively charged graphene derivatives and triiodide anions. the resulting composites were characterized by x-ray photoemission spectroscopy, uv-spectroscopy, raman spectroscopy and scanning electron microscopy. the antibacterial pote ... | 2016 | 26843066 |
| prevalence of urinary tract infection and antimicrobial susceptibility among diabetic patients with controlled and uncontrolled glycemia in kuwait. | diabetic patients have higher risk of urinary tract infection (uti). in the present study, we investigated the impact of glycemic control in diabetic patients on uti prevalence, type of strains, and their antimicrobial drugs susceptibility. this study was conducted on urine samples from 722 adult diabetic patients from which 252 (35%) samples were positive for uropathogens. most uti cases occurred in the uncontrolled glycemic group (197 patients) versus 55 patients with controlled glycemia. high ... | 2015 | 26844231 |
| [molecular characterization of an outbreak of carbapenem-resistant klebsiella pneumoniae in a tertiary care hospital in medellín, colombia]. | resistance to carbapenems is considered to represent a serious threat to public health at the global level, since these antibiotics are one of the last therapeutic options for the treatment of multidrug-resistant bacteria. molecular characterization of outbreaks due to resistant bacteria provides information that can be used in the design of infection control strategies. | 2017 | 26844438 |
| differences in the pharmacodynamics of ceftaroline against different species of enterobacteriaceae studied in an in vitro pharmacokinetic model of infection. | dose-ranging experiments were performed to study the pharmacodynamics of ceftaroline against enterobacteriaceae. | 2016 | 26846209 |
| kpc enzymes in the uk: an analysis of the first 160 cases outside the north-west region. | klebsiella pneumoniae carbapenemases (kpcs) have been increasingly reported in the uk since 2003. we analysed patient and isolate data for kpc-positive bacteria confirmed by the national reference laboratory from uk laboratories from august 2003 to august 2014, excluding north-west england, where the epidemiology has previously been studied. | 2016 | 26846210 |
| national epidemiology of carbapenem-resistant and extensively drug-resistant gram-negative bacteria isolated from blood samples in china in 2013. | although antimicrobial resistance poses a great challenge to clinicians in china, there are limited antimicrobial resistance data on gram-negative bacteria nationwide. we investigated the phenotypic characteristics of carbapenem-resistant escherichia coli (crec) and klebsiella pneumoniae (crkp) as well as extensively drug-resistant strains of pseudomonas aeruginosa (xdrpa) and acinetobacter baumannii (xdrab) isolated from blood cultures in china. data were collected on 24113 isolates from the ch ... | 2016 | 26846351 |
| multidrug resistance related to biofilm formation in acinetobacter baumannii and klebsiella pneumoniae clinical strains from different pulsotypes. | the emergence of acinetobacter baumannii and klebsiella pneumoniae strains in the hospital environment has been associated with the presence of multiple genetic elements, virulence factors and the ability to form biofilms. this study evaluated the biofilm formation ability of clinical and environmental a. baumannii and k. pneumoniae strains, isolated from various sources and presenting different molecular characteristics, resistance profiles and pulsed-field gel electrophoresis patterns. fifty-t ... | 2016 | 26846651 |
| simple and rapid sample preparation system for the molecular detection of antibiotic resistant pathogens in human urine. | antibiotic resistance in urinary tract infections (utis) can cause significant complications without quick detection and appropriate treatment. we describe a new approach to capture, concentrate and prepare amplification-ready dna from antibiotic resistant bacteria in human urine samples. klebsiella pneumoniae nctc13443 (bla ctx-m-15 positive) spiked into filtered human urine was used as a model system. bacteria were captured using anion exchange diaethylaminoethyl (deae) magnetic microparticles ... | 2016 | 26846875 |
| targeting an essential gtpase obg for the development of broad-spectrum antibiotics. | a promising new drug target for the development of novel broad-spectrum antibiotics is the highly conserved small gtpase obg (yhbz, cgta), a protein essential for the survival of all bacteria including neisseria gonorrhoeae (gc). gc is the agent of gonorrhea, a prevalent sexually transmitted disease resulting in serious consequences on reproductive and neonatal health. a preventive anti-gonorrhea vaccine does not exist, and options for effective antibiotic treatments are increasingly limited. to ... | 2016 | 26848972 |
| risk factors and outcomes of carbapenem-resistant klebsiella pneumoniae infections in liver transplant recipients. | 2016 | 26849961 | |
| spectrum of excess mortality due to carbapenem-resistant klebsiella pneumoniae infections. | patients infected or colonized with carbapenem-resistant klebsiella pneumoniae (crkp) are often chronically and acutely ill, which results in substantial mortality unrelated to infection. therefore, estimating excess mortality due to crkp infections is challenging. the consortium on resistance against carbapenems in k. pneumoniae (crackle) is a prospective multicenter study. here, patients in crackle were evaluated at the time of their first crkp bloodstream infection (bsi), pneumonia or urinary ... | 2016 | 26850824 |
| predictors of outcome in icu patients with septic shock caused by klebsiella pneumoniae carbapenemase-producing k. pneumoniae. | the aim of this study was to identify factors associated with mortality in intensive care unit patients with klebsiella pneumoniae carbapenemase-producing k. pneumoniae (kpc-kp) septic shock. a retrospective analysis of intensive care unit patients with kpc-kp infection and septic shock observed in a large teaching hospital from november 2010 to december 2014 was performed. a total of 111 patients were included in the study. the most frequent source of infection was unknown-focus bacteraemia in ... | 2016 | 26850826 |
| ht-spoti: a rapid drug susceptibility test (dst) to evaluate antibiotic resistance profiles and novel chemicals for anti-infective drug discovery. | antibiotic resistance is one of the major threats to global health and well-being. the past decade has seen an alarming rise in the evolution and spread of drug-resistant strains of pathogenic microbes. the emergence of extensively drug resistant (xdr) strains of mycobacterium tuberculosis and antimicrobial resistance among the eskape (enterococcus faecium, staphylococcus aureus, klebsiella pneumoniae, acinetobacter baumanii, pseudomonas aeruginosa, and enterobacter species) as well as fungal pa ... | 2016 | 26855282 |
| oestrogen promotes healing in a bacterial lps model of delayed cutaneous wound repair. | wound infection is a major clinical problem, yet understanding of bacterial host interactions in the skin remains limited. microbe-derived molecules, known as pathogen-associated molecular patterns, are recognised in barrier tissues by pattern-recognition receptors. in particular, the pathogen-associated molecular pattern, lipopolysaccharide (lps), a component of microbial cell walls and a specific ligand for toll-like receptor 4, has been widely used to mimic systemic and local infection across ... | 2016 | 26855364 |
| antibiotic resistance in enterobacteriaceae: mechanisms and clinical implications. | resistance of the enterobacteriaceae to antibiotics, especially of the β lactam type, is increasingly dominated by the mobilization of continuously expressed single genes that encode efficient drug modifying enzymes. strong and ubiquitous selection pressure has seemingly been accompanied by a shift from "natural" resistance, such as inducible chromosomal enzymes, membrane impermeability, and drug efflux, to the modern paradigm of mobile gene pools that largely determine the epidemiology of moder ... | 2016 | 26858245 |
| modulation of the antibiotic activity against multidrug resistant strains of 4-(phenylsulfonyl) morpholine. | the compound 4-(phenylsulfonyl) morpholine belongs to the class of sulfonamides, which are widely used in the treatment of a large number of diseases caused by microorganisms. this compound has a morpholine group, which is also known for its antimicrobial properties. the aim of the present study was to investigate the antimicrobial and modulating activity of 4-(phenylsulfonyl) morpholine against standard and multi-resistant strains of staphylococcus aureus, escherichia coli, klebsiella pneumonia ... | 2016 | 26858536 |
| identification of key amino acid residues modulating intracellular and in vitro microcin e492 amyloid formation. | microcin e492 (mcce492) is a pore-forming bacteriocin produced and exported by klebsiella pneumoniae ryc492. besides its antibacterial activity, excreted mcce492 can form amyloid fibrils in vivo as well as in vitro. it has been proposed that bacterial amyloids can be functional playing a biological role, and in the particular case of mcce492 it would control the antibacterial activity. mcce492 amyloid fibril's morphology and formation kinetics in vitro have been well-characterized, however, it i ... | 2016 | 26858708 |
| temperature-dependent gentamicin resistance of francisella tularensis is mediated by uptake modulation. | gentamicin (gm) is an aminoglycoside commonly used to treat bacterial infections such as tularemia - the disease caused by francisella tularensis. in addition to being pathogenic, f. tularensis is found in environmental niches such as soil where this bacterium likely encounters gm producers (micromonospora sp.). here we show that f. tularensis exhibits increased resistance to gm at ambient temperature (26°c) compared to mammalian body temperature (37°c). to evaluate whether f. tularensis was les ... | 2016 | 26858709 |
| the carbapenem-resistant enterobacteriaceae threat is growing: ndm-1 epidemic at a training hospital in turkey. | recently, new carbapenemases in enterobacteriaceae strains and non-fermentative gram-negative bacilli have been reported. the new delhi metallo-β-lactamase-1 (ndm-1) is a major problem around the world. the purpose of this article is to address the ndm-1 klebsiella pneumoniae epidemic detected in eight cases in our hospital. | 2016 | 26860360 |
| understanding the antimicrobial activity behind thin- and thick-rolled copper plates. | the aim of this study was to compare the antibacterial properties of the surfaces of copper plates that were rolled to a thickness of 25 and 100 μm. differences in topology of 25- and 100-μm-thick copper plates were studied using scanning electron microscopy (sem), atomic force microscopy (afm), and x-ray diffraction (xrd). antibacterial activity of the copper surfaces was tested against strains of staphylococcus aureus, escherichia coli, klebsiella pneumoniae, pseudomonas aeruginosa, listeria m ... | 2016 | 26860943 |
| persistence of nasal colonization with human pathogenic bacteria and associated antimicrobial resistance in the german general population. | the nares represent an important bacterial reservoir for endogenous infections. this study aimed to assess the prevalence of nasal colonization by different important pathogens, the associated antimicrobial susceptibility and risk factors. we performed a prospective cohort study among 1878 nonhospitalized volunteers recruited from the general population in germany. participants provided nasal swabs at three time points (each separated by 4-6 months). staphylococcus aureus, enterobacteriaceae and ... | 2015 | 26862431 |
| il-36γ is secreted in microparticles and exosomes by lung macrophages in response to bacteria and bacterial components. | interleukin-36 is a family of novel interleukin-1-like proinflammatory cytokines that are highly expressed in epithelial tissues and several myeloid-derived cell types. like those of classic interleukin-1 cytokines, the secretion mechanisms of interleukin-36 are not well understood. interleukin-36γ secretion in dermal epithelial cells requires adenosine 5'-triphosphate, which suggests a nonclassical mechanism of secretion. in this study, murine pulmonary macrophages and human alveolar macrophage ... | 2016 | 26864267 |
| study of silver nanoparticles synthesized by acidophilic strain of actinobacteria isolated from the of picea sitchensis forest soil. | in the present work the acidophilic actinobacteria strain was used as a novel reducing agent for the cheap, green and single-step synthesis of nanostructure silver particles. structural, morphological and optical properties of the synthesized nanoparticles have been characterized by spectroscopy, dynamic light scattering and electron microscopy approach. the antimicrobial activity of silver nanoparticles against clinical strains such as staphylococcus aureus, bacillus subtilis, escherichia coli, ... | 2016 | 26864807 |
| use of whole-genome sequencing to trace, control and characterize the regional expansion of extended-spectrum β-lactamase producing st15 klebsiella pneumoniae. | the study describes the transmission of a ctx-m-15-producing st15 klebsiella pneumoniae between patients treated in a single center and the subsequent inter-institutional spread by patient referral occurring between may 2012 and september 2013. a suspected epidemiological link between clinical k. pneumoniae isolates was supported by patient contact tracing and genomic phylogenetic analysis from may to november 2012. by may 2013, a patient treated in three institutions in two cities was involved ... | 2016 | 26864946 |
| multidrug-resistant gram-negative bacterial infections in the hospital setting: overview, implications for clinical practice, and emerging treatment options. | the increasing prevalence of infections due to multidrug-resistant (mdr) gram-negative bacteria constitutes a serious threat to global public health due to the limited treatment options available and the historically slow pace of development of new antimicrobial agents. infections due to mdr strains are associated with increased morbidity and mortality and prolonged hospitalization, which translates to a significant burden on healthcare systems. in particular, mdr strains of enterobacteriaceae ( ... | 2016 | 26866778 |
| in vitro susceptibility of multi-drug resistant pseudomonas aeruginosa and extended-spectrum β-lactamase-producing klebsiella pneumoniae isolated from clinical specimens at bugando medical centre, tanzania to piperacillin-tazobactam. | pseudomonas spp. and klebsiella pneumoniae are common causes of serious health care associated infections (hcais) worldwide. the treatment options for infections caused by multi-drug resistant (mdr) organisms are limited to tigecycline and carbapenems. a total of 172 isolates of multi-drug resistant pseudomonas. spp and extended-spectrum β- (esbl) producing klebsiella pneumoniae isolated from clinical specimens at the bugando medical centre were tested for their in vitro susceptibility to pipera ... | 2014 | 26867273 |
| detection of new delhi metallo-beta-lactamase-1-producing klebsiella pneumoniae at a general hospital in thailand. | the purpose of this study was to detect carbapenemase genes in clinical isolates of carbapenem-resistant enterobacteriaceae (cre) obtained from patients admitted to hua-hin hospital, prachuab khiri khan province, thailand between january and december 2014. screening of cre was initially determined using disk diffusion method, and subsequently using modified hodge test (mht). multiplex pcr was employed to amplify carbapenemase genes, blaimp, blaoxa-48, blandm blakpc, and blavim. of the 624 clinic ... | 2015 | 26867361 |
| carbapenem-resistant klebsiella pneumoniae colonization in pediatric and neonatal intensive care units: risk factors for progression to infection. | little is known about factors associated with carbapenem-resistant klebsiella pneumoniae infections in pediatric patients, who are initally colonized with carbapenem-resistant klebsiella pneumoniae. | 2016 | 26867474 |
| investigation of a suspected nosocomial transmission of blakpc3-mediated carbapenem-resistant klebsiella pneumoniae by whole genome sequencing. | whole genome sequencing (wgs) was compared to pulse-field gel electrophoresis (pfge) of xbai-digested genomic dna, as methods by which to evaluate a potential transmission of carbapenem-resistant klebsiella pneumoniae between 2 hospital inpatients. pfge result demonstrated only 1-band difference between the isolates, suggesting probable relatedness. in contrast, while wgs data demonstrated the same sequence type and very similar chromosomal sequences, over 20 single nucleotide variants were iden ... | 2016 | 26867964 |
| draft genome sequence of a klebsiella pneumoniae carbapenemase-positive sequence type 111 pseudomonas aeruginosa strain. | here, we report the draft genome sequence of a sequence type 111 pseudomonas aeruginosa strain isolated in 2014 from a patient at the nih clinical center. this p. aeruginosa strain exhibits pan-drug resistance and harbors the blakpc-2 gene, encoding the klebsiella pneumoniae carbapenemase enzyme, on a plasmid. | 2016 | 26868386 |
| quantitation of polymyxin-lipopolysaccharide interactions using an image-based fluorescent probe. | the frequency of polymyxin-resistant pathogenic gram-negative bacteria appearing in the clinic is increasing, and the consequences are largely mediated by modification of lipopolysaccharide (lps) in the outer membrane. as polymyxins exert their antibacterial effect by binding to lps, understanding their mode of binding will prove highly valuable for new antibiotic discovery. in this study, we assess the potential of mips-9451, a fluorescent polymyxin analogue designed for imaging studies, as a f ... | 2016 | 26869441 |
| characteristics of extended-spectrum β-lactamase (esbl)- and pampc beta-lactamase-producing enterobacteriaceae of water samples in tunisia. | the presence of extended-spectrum beta-lactamase and plasmid-mediated ampc beta-lactamase producing enterobacteriaceae (esbl-eb and pampc-eb, respectively) was analyzed in 57 wastewater and 57 surface-water samples in tunisia. twenty-four of the 57 wastewater samples (42.1%) and one of the 57 surface-water samples (1.7%, a river that received effluents of a wastewater-treatment-plant) contained esbl-eb or pampc-eb; one esbl/pampc-eb per positive sample was further characterized. beta-lactamase g ... | 2016 | 26871556 |
| bacteremia in children hospitalized with respiratory syncytial virus infection. | the risk of bacteremia is considered low in children with acute bronchiolitis. however the rate of occult bacteremia in infants with rsv infection is not well established. the aim was to determine the actual rate and predictive factors of bacteremia in children admitted to hospital due to confirmed rsv acute respiratory illness (ari), using both conventional culture and molecular techniques. | 2016 | 26872131 |
| whole genome sequence of klebsiella pneumoniae u25, a hypermucoviscous, multidrug resistant, biofilm producing isolate from india. | klebsiella pneumoniae u25 is a multidrug resistant strain isolated from a tertiary care hospital in chennai, india. here, we report the complete annotated genome sequence of strain u25 obtained using pacbio rsii. this is the first report of the whole genome of k. pneumoniaespecies from chennai. it consists of a single circular chromosome of size 5,491,870-bp and two plasmids of size 211,813 and 172,619-bp. the genes associated with multidrug resistance were identified. the chromosome of u25 was ... | 2016 | 26872343 |
| identification and characterization of holgh15: the holin of staphylococcus aureus bacteriophage gh15. | holins are phage-encoded hydrophobic membrane proteins that spontaneously and non-specifically accumulate and form lesions in the cytoplasmic membrane. the orf72 gene (also designated holgh15) derived from the genome of the staphylococcus aureus phage gh15 was predicted to encode a membrane protein. an analysis indicated that the protein encoded by holgh15 potentially consisted of two hydrophobic transmembrane helices. this protein exhibited the structural characteristics of class ii holins and ... | 2016 | 26873847 |
| molecular detection and antimicrobial resistance of klebsiella pneumoniae from house flies (musca domestica) in kitchens, farms, hospitals and slaughterhouses. | identifying disease vectors and pathogens is one of the key steps in controlling vector-borne diseases. this study investigated the possible role of house flies (musca domestica) as vectors in the transmission of klebsiella pneumoniae in chaharmahal va bakhtiari and isfahan provinces of iran. house flies were captured from household kitchens, cattle farms, chicken farms, animal hospitals, human hospitals and slaughterhouses. isolation of k. pneumoniae from external surfaces and guts of the flies ... | 2017 | 26876433 |
| trends in the distribution and antimicrobial susceptibility of causative pathogens of device-associated infection in korean intensive care units from 2006 to 2013: results from the korean nosocomial infections surveillance system (konis). | for all countries, information on pathogens causing healthcare-associated infections is important in order to develop proper strategies for preventing and treating nosocomial infections. | 2016 | 26876746 |
| nosocomial infection and its molecular mechanisms of antibiotic resistance. | nosocomial infection is a kind of infection, which is spread in various hospital environments, and leads to many serious diseases (e.g. pneumonia, urinary tract infection, gastroenteritis, and puerperal fever), and causes higher mortality than community-acquired infection. bacteria are predominant among all the nosocomial infection-associated pathogens, thus a large number of antibiotics, such as aminoglycosides, penicillins, cephalosporins, and carbapenems, are adopted in clinical treatment. ho ... | 2016 | 26877142 |
| salicylanilide n-monosubstituted carbamates: synthesis and in vitro antimicrobial activity. | the research of innovative antimicrobial agents represents a cutting edge topic. hence, we synthesized and characterised novel salicylanilide n-monosubstituted carbamates. twenty compounds were evaluated in vitro against eight bacterial strains and eight fungal species. the lowest minimum inhibitory concentrations (mics) were found to be ⩽0.49 μm. genus staphylococcus, including methicillin-resistant staphylococcus aureus, and fungus trichophyton mentagrophytes showed uniformly the highest rate ... | 2016 | 26879856 |
| bacteriospermia and its impact on basic semen parameters among infertile men. | introduction. semen analysis is considered as the surrogate marker for male fecundity while assessing infertile men. there are several reasons for altered semen quality and bacteriospermia could be one among them. thereby the aim of our work is to study the semen culture and its impact on semen parameters among infertile men. materials and methods. semen samples were collected from men attending infertility clinic. semen parameters were analysed based on who guidelines. also, samples were subjec ... | 2016 | 26880908 |
| risk factors and outcomes for carbapenem-resistant klebsiella pneumoniae isolation, stratified by its multilocus sequence typing: st258 versus non-st258. | a "high risk" clone of carbapenem-resistant klebsiella pneumoniae (crkp) identified by multilocus sequence typing (mlst) as sequence type (st) 258 has disseminated worldwide. as the molecular epidemiology of the cre pandemic continues to evolve, the clinical impact of non-st258 strains is less well defined. we conducted an epidemiological investigation of crkp based on strains mlst. among 68 crkp patients, 61 were st258 and 7 belonged to non-st258. klebsiella pneumoniae st258 strains were signif ... | 2016 | 26885543 |
| metabolic engineering of klebsiella pneumoniae and in silico investigation for enhanced 2,3-butanediol production. | to improve the production of 2,3-butanediol (2,3-bd) in klebsiella pneumoniae, the genes related to the formation of lactic acid, ethanol, and acetic acid were eliminated. | 2016 | 26886192 |
| structure, toxicity and antibiotic activity of gramicidin s and derivatives. | development of new antibiotics is declining whereas antibiotic resistance is rising, heralding a post-antibiotic era. antimicrobial peptides such as gramicidin s (gs), exclusively topically used due to its hemolytic side-effect, could still be interesting as therapeutic compounds. by modifying the amino-acid composition of gs, we synthesized gs analogues. we now show that derivative vk7 has a lower mic (7.8-31.2 μg/ml, median 15.6 μg/ml) against strains of multi-drug resistant (mdr) klebsiella p ... | 2016 | 26886453 |
| photodynamic inactivation of klebsiella pneumoniae biofilms and planktonic cells by 5-aminolevulinic acid and 5-aminolevulinic acid methyl ester. | the treatment of klebsiella pneumoniae, particularly extended-spectrum β-lactamase (esbl)-producing k. pneumoniae, is currently a great challenge. photodynamic antimicrobial chemotherapy is a promising approach for killing antibiotic-resistant bacteria. the aim of this study was to evaluate the capacity of 5-aminolevulinic acid (5-ala) and its derivative 5-ala methyl ester (mal) in the presence of white light to cause photodynamic inactivation (pdi) of k. pneumoniae planktonic and biofilm cells. ... | 2016 | 26886586 |
| a case of recurrent aortic rupture associated with klebsiella pneumoniae pericarditis treated by two separate aortic operations. | a 49-year-old female presented with severe dyspnea. she was diagnosed with cardiac tamponade combined with ascending aortic pseudoaneurysm and rupture, which was caused by klebsiella pneumoniae infection. this extremely rare condition was managed by an emergency pericardiostomy and two separate aortic operations. antibiotics active for the k. pneumoniae isolate were used throughout. the patient was well for nine months after discharge and continues to be followed up for signs of possible reinfec ... | 2016 | 26889448 |
| recent advances in biosensor based diagnosis of urinary tract infection. | urinary tract infections (utis) are potentially life threatening infections that are associated with high rates of incidence, recurrence and mortality. utis are characterized by several chronic infections which may lead to lethal consequences if left undiagnosed and untreated. the uropathogens are consistent across the globe. the most prevalent uropathogenic gram negative bacteria are escherichia coli, proteus mirabilis, pseudomonas aeruginosa, klebsiella pneumonia. early detection and precise d ... | 2016 | 26890825 |
| pathogens in ornamental waters: a pilot study. | in parks, ornamental waters of easy access and populated with animals are quite attractive to children and yet might hide threats to human health. the present work focuses on the microbiota of the ornamental waters of a lisboa park, characterized during 2015. the results show a dynamic microbiota integrating human pathogens such as klebsiella pneumoniae, aeromonas spp. and enterobacter spp., and also antibiotic resistant bacteria. k. pneumoniae and aeromonas spp. were present as planktonic and b ... | 2016 | 26891309 |
| effect of a metalloantibiotic produced by pseudomonas aeruginosa on klebsiella pneumoniae carbapenemase (kpc)-producing k. pneumoniae. | multidrug-resistant organisms (mdro) are a great problem in hospitals, where thousands of people are infected daily, with the occurrence of high mortality rates, especially in infections caused by klebsiella pneumoniae carbapenemase-producing k. pneumoniae (kpc-producing kpn). the challenge is to find new compounds that can control kpc producing-kpn infections. the aim of this study was to evaluate the antibiotic activity of the f3d fraction produced by the pseudomonas aeruginosa lv strain again ... | 2016 | 26891742 |
| non-susceptibility of escherichia coli, klebsiella spp., pseudomonas spp., streptococcus pneumoniae and staphylococcus aureus in the uk: temporal trends in england, northern ireland, scotland and wales. | to monitor and compare trends in the non-susceptibility of bloodstream isolates of pathogens to key antibiotics in the constituent countries of the uk between 2010 and 2014. | 2016 | 26892779 |
| surface antigens contribute differently to the pathophysiological features in serotype k1 and k2 klebsiella pneumoniae strains isolated from liver abscesses. | the virulence role of surface antigens in a single serotype of klebsiella pneumoniae strain have been studied, but little is known about whether their contribution will vary with serotype. | 2016 | 26893615 |
| prevalence of lrti in patients presenting with productive cough and their antibiotic resistance pattern. | to find out the prevalence of lower respiratory tract infection (lrti) such as bacterial, fungal, mycobacterial infections etc. in patients with productive cough of duration less than 15 days and to rule out the patients having previous history of tuberculosis or having treatment of tuberculosis. | 2016 | 26894065 |
| granzymes a and b regulate the local inflammatory response during klebsiella pneumoniae pneumonia. | klebsiella pneumoniae is a common cause of hospital-acquired pneumonia. granzymes (gzms), mainly found in cytotoxic lymphocytes, have been implicated as mediators of infection and inflammation. we here sought to investigate the role of gzma and gzmb in the host response to k. pneumoniae-induced airway infection and sepsis. for this purpose, pneumonia was induced in wild-type (wt) and gzma-deficient (gzma-/-), gzmb-/- and gzmaxb-/- mice by intranasal infection with k. pneumoniae. in wt mice, gzma ... | 2016 | 26894590 |
| tea tree oil nanoemulsions for inhalation therapies of bacterial and fungal pneumonia. | tea tree oil (tto) is a natural essential oil with strong antimicrobial efficacy and little drug resistance. however, the biomedical applications of tto are limited due to its hydrophobicity and formulation problems. here, we prepared an inhalable tto nanoemulsion (nanotto) for local therapies of bacterial and fungal pneumonia. the optimal formulation of nanottos consisted of tto/cremophor el/water with a mean size of 12.5nm. the nanottos showed strong in vitro antimicrobial activities on escher ... | 2016 | 26895502 |
| outbreak of plasmid-mediated ndm-1-producing klebsiella pneumoniae st105 among neonatal patients in yunnan, china. | in the past decade, the carbapenemase-producing enterobacteriaceae (cpe) have been reported worldwide. emergence of carbapenemase-producing strains among enterobacteriaceae has been a challenge for treatment of clinical infection. the present study was undertaken to investigate the characteristics of carbapenem-resistant klebsiella pneumoniae recovered from an outbreak that affected 17 neonatal patients in neonatal intensive care unit (nicu) of kunming city maternal and child health hospital, wh ... | 2016 | 26896089 |
| i.v. minocycline revisited for infections caused by multidrug-resistant organisms. | the evidence supporting the potential use of i.v. minocycline for serious infections caused by multidrug-resistant organisms (mdros) is summarized. | 2016 | 26896499 |