Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| acute otitis media in children. | acute otitis media is a common childhood infection. prompt diagnosis and appropriate treatment are very important. | 2017 | 28707578 |
| nasopharyngeal microbiota in infants and changes during viral upper respiratory tract infection and acute otitis media. | interferences between pathogenic bacteria and specific commensals are known. we determined the interactions between nasopharyngeal microbial pathogens and commensals during viral upper respiratory tract infection (uri) and acute otitis media (aom) in infants. | 2017 | 28708872 |
| development of antimicrobial nanoemulsion-based delivery systems against selected pathogenic bacteria using a thymol rich thymus daenensis essential oil. | thymol rich medicinal plants have been used in traditional medicine to relieve infectious diseases. however, the application of essential oils as medicine is limited by its low water-solubility and high vapor pressure. the objective of this study was to produce stable nanoemulsions of thymus daenensis oil in water by preventing ostwald ripening and phase separation. | 2017 | 28714250 |
| a randomized, controlled study of a fully liquid dtap-ipv-hb-prp-t hexavalent vaccine for primary and booster vaccinations of healthy infants and toddlers in latin america. | hexavalent diphtheria (d)-tetanus (t)-acellular pertussis (ap)-inactivated poliovirus (ipv)-hepatitis b (hb)-haemophilus influenzae type b (prp-t)-containing vaccines are increasingly the standard of care. this study evaluated the primary series (nct01177722) and booster (nct01444781) of a fully liquid dtap-ipv-hb-prp-t vaccine in latin america. | 2017 | 28719500 |
| determining optimal dosing regimen of oral administration of dicloxacillin using monte carlo simulation. | dicloxacillin, a semisynthetic isoxazolyl penicillin, exhibits antimicrobial activity against a wide variety of gram-positive bacteria, as well as stability against penicillinases and low level of toxicity. the objective of this study was to obtain optimal dosing regimen of oral administration of dicloxacillin by analyzing the pharmacokinetic (pk) index in healthy volunteers and in vitro antibacterial activity by using monte carlo simulation. | 2017 | 28721014 |
| haemophilus influenzae serotype f endocarditis and septic arthritis. | haemophilus influenzae represents gram-negative coccobacilli which can cause endocarditis, meningitis, septicemia, pneumonia, septic arthritis. h.influenzae exists as encapsulated and unencapsulated (non-typeable) strains. non-typeable h.influenzae are emerging pathogens especially in elderly population. we report a case of a 73 year old woman with bacteremia, endocarditis and septic arthritis due to h.influenzae serotype f. this case emphasizes the clinical features and the key elements of diag ... | 2017 | 28725560 |
| selective igm deficiency: clinical and laboratory features of 17 patients and a review of the literature. | primary selective igm deficiency (sigmd) is a primary immunodeficiency with unclear pathogenesis and a low number of published cases. | 2017 | 28730517 |
| recombinant truncated e protein as a new vaccine candidate against nontypeable h. influenzae: its expression and immunogenic evaluation. | protein e (pe) is a conserved entity observed in both nontypeable haemophilus influenzae (nthi) and encapsulated h. influenzae. this is a small surface lipoprotein, consisting of only 160 amino acids, involved in the adhesion of h. influenzae to various types of epithelial cells. a 384-bp-long fragment from nthi pe was cloned into the prokaryotic expression vector pbad-giiia. the recombinant protein was expressed with arabinose and then purified by affinity purification on an ni-nta agarose matr ... | 2017 | 28735080 |
| economic evaluations of haemophilus influenzae type b (hib) vaccine: a systematic review. | the world health organization (who) recommends the use of haemophilus influenzae type b (hib) conjugate vaccines, but china and thailand have not used hib vaccination in their national immunization programs. this systematic review aimed to update published economic evaluations of hib vaccinations and to determine factors that potentially affected their cost-effectiveness. | 2017 | 28737468 |
| [neisseria gonorrhoeae: an infrequent causal agent of periorbital cellulitis in a child]. | periorbital cellulitis is a very common disease in pediatric population, it describes an infection involving the adjacent soft tissues anterior to the orbital septum. pathogens involved are generally gram-positive cocci after introduction of haemophilus influenzae vaccine. we report a case of neisseria gonorrhoeae periorbital cellulitis associated with bacterial conjunctivitis in a child. there are few cases reported in the literature with this microorganism as the causal agent. with this case, ... | 2017 | 28737877 |
| transmission fitness in co-colonization and the persistence of bacterial pathogens. | humans are often colonized by polymorphic bacteria such as streptococcus pneumoniae, bordetella pertussis, staphylococcus aureus, and haemophilus influenzae. two co-colonizing pathogen clones may interact with each other upon host entry and during within-host dynamics, ranging from competition to facilitation. here we examine the significance of these exploitation strategies for bacterial spread and persistence in host populations. we model sis epidemiological dynamics to capture the global beha ... | 2017 | 28741105 |
| induction of β-lactamase activity and decreased β-lactam susceptibility by co2 in clinical bacterial isolates. | antimicrobial susceptibility testing of clinical isolates is a crucial step toward appropriate treatment of infectious diseases. the clinical isolate francisella philomiragia 14iuhpl001, recently isolated from a 63-year-old woman with atypical pneumonia, featured decreased susceptibility to β-lactam antibiotics when cultivated in 5% co2. quantitative β-lactamase assays demonstrated a significant (p < 0.0001) increase in enzymatic activity between bacteria cultivated in 5% co2 over those incubate ... | 2017 | 28744482 |
| determination of disk diffusion and mic quality control ranges for nafithromycin (wck 4873), a new lactone-ketolide. | disk diffusion and mic quality control (qc) ranges were determined for nafithromycin, a new lactone-ketolide, following completion of a nine-laboratory, clinical and laboratory standards institute (clsi) m23-defined tier 2 study. five qc strains consistent with the spectrum of activity of nafithromycin were tested: staphylococcus aureus atcc 25923 (disk only), s. aureus atcc 29213 (broth only), enterococcus faecalis atcc 29212 (broth only), streptococcus pneumoniae atcc 49619 (disk and broth), a ... | 2017 | 28747372 |
| serum antibody response to moraxella catarrhalis proteins in stringently defined otitis prone children. | moraxella catarrhalis (mcat) is a frequent pathogen of acute otitis media (aom) in young children. here we prospectively assessed naturally-induced serum antibodies to four mcat vaccine candidate proteins in stringently defined otitis prone (sop) and non-otitis prone (nop) children age 6-36months old following nasopharyngeal (np) colonization, at onset of aom and convalescence from aom. | 2017 | 28755833 |
| haemophilus influenzae type b meningitis in a vaccinated and immunocompetent child. | 2017 | 28757297 | |
| progress in definition, prevention and treatment of fungal infections in cystic fibrosis. | cystic fibrosis (cf) is a chronic lethal multi-system condition; however, most of the morbidity and mortality is dependent on the status of the respiratory system. progressive respiratory decline is mediated by chronic infection and inflammation, punctuated by important acute events known as pulmonary exacerbations which can lead to accelerated decline. the main bacterial species causing infections include pseudomonas aeruginosa, staphylococcus aureus, haemophilus influenzae and achromobacter xy ... | 2017 | 28762125 |
| frequency of acute otitis media in children under 24 months of age before and after the introduction of the 10-valent pneumococcal conjugate vaccine into the national immunization program in chile. | streptococcus pneumoniae is the leading cause of acute otitis media (aom). pneumococcal non-typable haemophilus influenzae protein d conjugate vaccine (pcv-10) was introduced to the chilean national immunization program (nip) in 2011. the aim of this study was to estimate the frequency of aom in children < 24 months of age attending the emergency department (ed) of hospital sótero del río (hsr) 4 years before and 4 years after the introduction of pcv-10 in the chilean nip. | 2017 | 28763425 |
| immunogenicity of nontypeable haemophilus influenzae outer membrane vesicles and protective ability in the chinchilla model of otitis media. | outer membrane vesicles (omvs) produced by gram-negative bacteria are enriched in several outer membrane components, including major and minor outer membrane proteins and lipooligosaccharide. we assessed the functional activity of nontypeable haemophilus influenzae (nthi) omv-specific antisera and the protective ability of nthi omvs as vaccine antigens in the chinchilla otitis media model. omvs were purified from three hmw1/hmw2-expressing nthi strains, two of which were also engineered to overe ... | 2017 | 28768669 |
| pd-l2 regulates b-1 cell antibody production against phosphorylcholine through an il-5-dependent mechanism. | b-1 cells produce natural abs which provide an integral first line of defense against pathogens while also performing important homeostatic housekeeping functions. in this study, we demonstrate that programmed cell death 1 ligand 2 (pd-l2) regulates the production of natural abs against phosphorylcholine (pc). naive pd-l2-deficient (pd-l2(-/-)) mice produced significantly more pc-reactive igm and iga. this afforded pd-l2(-/-) mice with selectively enhanced protection against pc-expressing nontyp ... | 2017 | 28768724 |
| vaccine-preventable diseases requiring hospitalization. | plain children often have lower immunization rates than non-plain children. penn state health children's hospital is a tertiary medical center with large nearby plain (amish and mennonite) communities. we sought to describe the characteristics of children hospitalized with vaccine-preventable diseases (vpds). we hypothesized that amish children would have a higher risk of vpds than non-amish children. | 2017 | 28768853 |
| fluorescence imaging of streptococcus pneumoniae with the helix pomatia agglutinin (hpa) as a potential, rapid diagnostic tool. | streptococcus pneumoniae is a common human pathogen and a major causal agent of life-threatening infections that can either be respiratory or non-respiratory. it is well known that the helix pomatia (edible snail) agglutinin (hpa) lectin shows specificity for terminal αgalnac residues present, among other locations, in the forssman pentasaccharide (αgalnac1→3βgalnac1→3αgal1→4βgal1→4βglc). based on experiments involving choline-independent mutants and different growth conditions, we propose here ... | 2017 | 28769901 |
| defective anti-polysaccharide igg vaccine responses in iga deficient mice. | we report that iga(-/-) mice exhibit specific defects in igg antibody responses to various polysaccharide vaccines (francisella tularensis lps and pneumovax), but not protein vaccines such as fluzone. this defect further included responses to polysaccharide-protein conjugate vaccines (prevnar and haemophilus influenzae type b-tetanus toxoid vaccine). in agreement with these findings, iga(-/-) mice were protected from pathogen challenge with protein- but not polysaccharide-based vaccines. interes ... | 2017 | 28774562 |
| characteristics of meningitis following transsphenoidal endoscopic surgery: a case series and a systematic literature review. | meningitis occurs in 0.8-1.5% of patients undergoing neurosurgery. the aim of the study was to evaluate the characteristics of meningitis after endoscopic endonasal transsphenoidal surgery (eets) comparing the findings retrieved to those highlighted by literature search. | 2017 | 28776163 |
| burden of lower respiratory infections in the eastern mediterranean region between 1990 and 2015: findings from the global burden of disease 2015 study. | we used data from the global burden of disease 2015 study (gbd) to calculate the burden of lower respiratory infections (lris) in the 22 countries of the eastern mediterranean region (emr) from 1990 to 2015. | 2017 | 28776246 |
| common bacterial isolates, clinical outcome and tb meningitis in children admitted at morogoro regional referral hospital, tanzania. | bacterial meningitis is still one of the major causes of deaths, disabilities, and mental retardation in children in morogoro region. to study the current meningitis burden, we evaluated the common bacterial isolates and clinical outcome of the disease in the region. | 2017 | 28776526 |
| molecular analysis of a locus for the biosynthesis and phase-variable expression of the lacto-n-neotetraose terminal lipopolysaccharide structure in neisseria meningitidis. | lipopolysaccharide (lps) is a major determinant of neisseria meningitidis virulence. a key feature of meningococcal lps is the phase-variable expression of terminal structures which are proposed to have disparate roles in pathogenesis. in order to identify the biosynthetic genes for terminal lps structures and the control mechanisms for their phase-variable expression, the lic2a gene, which is involved in lps biosynthesis in haemophilus influenzae, was used as a hybridization probe to identify a ... | 1995 | 28776847 |
| cloning and molecular analysis of the gale gene of neisseria meningitidls and its role in lipopolysaccharide biosynthesis. | the gale gene from haemophilus influenzae was used as a hybridization probe for the gale gene of neisseria meningitidis group b, identifying two different homologous loci. each of the loci was cloned and nucleotide sequence analysis revealed that both loci contained sequences similar to gale. one contained a functional gale gene and mapped to the capsule biosynthetic locus. the second contained only a partial gale-coding sequence, which did not express a functional gene product. a gale mutant me ... | 1993 | 28776852 |
| a third family of allelic hsd genes in salmonella enterica: sequence comparisons with related proteins identify conserved regions implicated in restriction of dna. | salmonella enterica serovar blegdam has a restriction and modification system encoded by genes linked to serb. we have cloned these genes, putative alleles of the hsd locus of escherichia coli k-12, and confirmed by the sequence similarities of flanking dna that the hsd genes of s. enterica serovar blegdam have the same chromosomal location as those of e. coli k-12 and salmonella enterica serovar typhimurium lt2. there is, however, no obvious similarity in their nucleotide sequences, and while t ... | 1996 | 28777503 |
| epidemiology of acute otitis media in the postpneumococcal conjugate vaccine era. | to study the epidemiology of acute otitis media (aom), especially the otitis-prone condition, during the pneumococcal conjugate vaccines 7 and 13 era. | 2017 | 28784702 |
| bacteriological incidence in pneumonia patients with pulmonary emphysema: a bacterial floral analysis using the 16s ribosomal rna gene in bronchoalveolar lavage fluid. | pulmonary emphysema is an important radiological finding in chronic obstructive pulmonary disease patients, but bacteriological differences in pneumonia patients according to the severity of emphysematous changes have not been reported. therefore, we evaluated the bacteriological incidence in the bronchoalveolar lavage fluid (balf) of pneumonia patients using cultivation and a culture-independent molecular method. japanese patients with community-acquired pneumonia (83) and healthcare-associated ... | 2017 | 28790814 |
| immunogenicity and safety of a booster dose of the 13-valent pneumococcal conjugate vaccine in children primed with the 10-valent or 13-valent pneumococcal conjugate vaccine in the czech republic and slovakia. | although both the 13-valent pneumococcal conjugate vaccine (pcv13) and the 10-valent pneumococcal non-typeable haemophilus influenzae protein d-conjugate vaccine (phid-cv) are widely used, it is unclear how interchangeable they are in terms of immunogenicity. | 2017 | 28797727 |
| surgical management of cervical spine deformity in chondrodysplasia punctata. | objective chondrodysplasia punctata (cdp), a rare skeletal dysplasia, can lead to cervical spine instability and deformity. however, an optimal neurosurgical intervention has yet to be established. thus, a retrospective study was conducted to assess the efficacy of various surgical interventions for children with cdp. methods the authors retrospectively reviewed 9 cases of cdp in which cervical decompression with or without posterior fusion was performed between april 2007 and may 2016. patient ... | 2017 | 28799855 |
| nontypeable haemophilus influenzae (nthi) directly interfere with the regulation of e-cadherin in lung epithelial cells. | loss of epithelial barriers characterized by reduction of e-cadherin is a hallmark of chronic obstructive pulmonary disease (copd). we investigated the effects of nontypeable haemophilus influenzae (nthi) infections, associated with acute exacerbations of chronic bronchitis, on the regulation of e-cadherin in host cells. nthi infection decreased e-cadherin mrna and protein-levels in lung epithelial cells. e-cadherin reduction was mediated by activation of the fibroblast growth factor 2 (fgf2), t ... | 2017 | 28802586 |
| simultaneous detection of 13 key bacterial respiratory pathogens by combination of multiplex pcr and capillary electrophoresis. | lower respiratory tract infections continue to pose a significant threat to human health. it is important to accurately and rapidly detect respiratory bacteria. to compensate for the limits of current respiratory bacteria detection methods, we developed a combination of multiplex polymerase chain reaction (pcr) and capillary electrophoresis (mpce) assay to detect thirteen bacterial pathogens responsible for lower respiratory tract infections, including streptococcus pneumoniae, haemophilus influ ... | 2017 | 28807095 |
| development of a highly resolved loop-mediated isothermal amplification method to detect the n526k ftsi mutation of β-lactamase-negative ampicillin-resistant haemophilus influenzae. | rapid and easy detection of sequence polymorphisms, including nucleotide point mutations of bacterial pathogens responsible for amino acid substitutions linked to drug resistance, is essential for the proper use of antimicrobial agents. here, a detection method using loop-mediated amplification (lamp) combined with amplification refractory mutation system (arms) to accurately distinguish a different single nucleotide in the target sequence was established, named arms-snp lamp. this procedure is ... | 2017 | 28807759 |
| reducing antibacterial development risk for gsk1322322 by exploring potential human dose regimens in nonclinical efficacy studies using immunocompetent rats. | directly testing proposed clinical dosing regimens in nonclinical studies can reduce risk during the development of novel antibacterial agents. optimal dosing regimens can be identified in animal models by testing recreated human pharmacokinetic profiles. an example of this approach is presented using continuous intravenous infusions of gsk1322322 in immunocompetent rats to evaluate recreated phase 1 human exposures in pneumonia models with streptococcus pneumoniae and haemophilus influenzae and ... | 2017 | 28807913 |
| beta-lactam resistance among haemophilus influenzae isolates in poland. | haemophilus influenzae is a human-specific gram-negative coccobacillus responsible for a significant number of respiratory tract infections and severe invasive infections such as meningitis and sepsis. the purpose of the study was to characterize the mechanisms of β-lactam resistance among polish h. influenzae isolates and to evaluate the methods applied. | 2017 | 28818575 |
| alloiococcus otitidis forms multispecies biofilm with haemophilus influenzae: effects on antibiotic susceptibility and growth in adverse conditions. | otitis media with effusion (ome) is a biofilm driven disease and commonly accepted otopathogens, such as haemophilus influenzae, streptococcus pneumonia, and moraxella catarrhalis, have been demonstrated to form polymicrobial biofilms within the middle ear cleft. however, alloiococcus otitidis (a. otitidis), which is one of the most commonly found bacteria within middle ear aspirates of children with ome, has not been described to form biofilms. the aim of this study was to investigate whether a ... | 2017 | 28824879 |
| cerebrospinal fluid cathelicidin correlates with the bacterial load and outcomes in childhood bacterial meningitis. | large cerebrospinal fluid (csf) bacterial load in bacterial meningitis (bm) relates to poor outcome. however, the antimicrobial peptide cathelicidin seems important to host defense. we studied how cathelicidin concentrations and bacterial load in csf relate in childhood bm and to what extent they may predict the disease outcome. | 2017 | 28827496 |
| be aware of immunogenic but not protective antigens: the actinobacillus pleuropneumoniae pala as an example. | identification of immunogenic antigens is an important step for the vaccine improvement of infectious diseases. previous studies indicated that actinobacillus pleuropneumoniae pala is homologous to a haemophilus influenzae protective antigen hi-pal (p6) protein. however, pala protein adversely affects the apx toxin-based subunit vaccine. in this study, we confirmed that recombinant pala is immunogenic in animals. mice immunized with bacterin and pala (bacpal) showed significantly less antibodies ... | 2017 | 28828973 |
| differences of lung microbiome in patients with clinically stable and exacerbated bronchiectasis. | molecular-based diagnostic techniques can compensate for the inherent limitations of culture-based microbiology and provide a more comprehensive description of an entire community of bacteria at a particular anatomical site. using culture-independent dna-based molecular techniques, the aim of the present study was to characterize, differentiate, and compare the composition of lower airway bacterial microbiome between clinically stable and acutely infected patients with bronchiectasis experiencin ... | 2017 | 28829833 |
| impact of meningitis on intelligence and development: a systematic review and meta-analysis. | we undertook a systematic review and meta-analysis to address the question "what is the impact of meningitis on iq and development." | 2017 | 28837564 |
| administering multiple injectable vaccines during a single visit-summary of findings from the accelerated introduction of inactivated polio vaccine globally. | in 2013, the world health organization's (who's) strategic advisory group of experts (sage) recommended that all 126 countries using only oral polio vaccine (opv) introduce at least 1 dose of inactivated polio vaccine (ipv) into their routine immunization schedules by the end of 2015. in many countries, the addition of ipv would necessitate delivery of multiple injectable vaccines (hereafter, "multiple injections") during a single visit, with infants receiving ipv alongside pentavalent vaccine ( ... | 2017 | 28838188 |
| experimental design and metabolic flux analysis tools to optimize industrially relevant haemophilus influenzae type b growth medium. | haemophilus influenzae type b (hib), a gram-negative capsulated bacterium, is a causative agent of meningitis worldwide. the capsular polysaccharide, a high molecular mass polymer consisting of the repeated units of the polyribosyl-ribitol-phosphate, is considered the main virulence factor and it is used as an antigen to vaccines, conjugated to a carrier protein. the industrial production of the polysaccharide requires the cultivation of hib in rich medium, which impacts process costs and produc ... | 2017 | 28840658 |
| [study on the classification of dominant pathogens related to febrile respiratory syndrome, based on the method of bayes discriminant analysis]. | objective: to understand the dominant pathogens of febrile respiratory syndrome (frs) patients in gansu province and to establish the bayes discriminant function in order to identify the patients infected with the dominant pathogens. methods: frs patients were collected in various sentinel hospitals of gansu province from 2009 to 2015 and the dominant pathogens were determined by describing the composition of pathogenic profile. significant clinical variables were selected by stepwise discrimina ... | 2017 | 28847061 |
| otitis media and nasopharyngeal colonization in ccl3(-/-) mice. | introduction. we previously found cc chemokine ligand 3 (ccl3) to be a potent effector of inflammation during otitis media (om): exogenous ccl3 rescues the om phenotype of tumor necrosis factor-deficient mice, and function of macrophages deficient in several innate immune molecules. to further delineate the role of ccl3 in om, we evaluated middle ear (me) responses of ccl3(-/-) mice to nontypeable haemophilus influenzae (nthi).materials and methods. ccl chemokine gene expression was evaluated in ... | 2017 | 28847849 |
| amino acid substitution in the major multi-drug efflux transporter protein acrb contributes to azithromycin low-susceptibility in haemophilus influenzae. | generally, clarithromycin-resistant haemophilus influenzae with a nonsense mutation in acrr exhibited susceptibility to azithromycin, although one strain was found to be nonsusceptible. we aimed to clarify the differences. this strain had an amino acid substitution, arg327ser, in acrb. introduction of this substitution into h. influenzae rd caused an increase in the mic of azithromycin, suggesting that this substitution contributed to nonsusceptibility. these findings indicate that azithromycin- ... | 2017 | 28848006 |
| cost-effectiveness analysis of a universal mass vaccination program with a phid-cv 2+1 schedule in malaysia. | currently, two pediatric pneumococcal conjugate vaccines are available in the private market of malaysia-13-valent pneumococcal conjugate vaccine (pcv13) and pneumococcal polysaccharide and non-typeable haemophilus influenzae protein d conjugate vaccine (phid-cv). this study aimed to evaluate the cost-effectiveness of a universal mass vaccination program with a phid-cv 2+1 schedule versus no vaccination or with a pcv13 2+1 schedule in malaysia. | 2017 | 28852326 |
| non-specific effects of measles, mumps, and rubella (mmr) vaccination in high income setting: population based cohort study in the netherlands. | objectives to investigate whether measles, mumps, and rubella (mmr) vaccine has positive non-specific effects in a high income setting and to compare rates of hospital admissions for infections between children aged ≤2 years who received live mmr vaccine and those who received an inactivated vaccine against diphtheria, tetanus, pertussis, polio, and haemophilus influenzae type b (dtap-ipv-hib) as their most recent vaccination.design nationwide population based cohort study.setting in the netherl ... | 2017 | 28855159 |
| polymicrobial bloodstream infection in an immunocompetent child. | 2017 | 28856764 | |
| fulminant haemophilus influenzae type a infection in a 4-year-old with previously undiagnosed asplenic heterotaxy. | the post hib-vaccine era has resulted in increased cases of invasive, non-hib, haemophilus influenzae infections. one of the encapsulated species, type a, displays the most similarity in virulence to type b. our patient, a fully-vaccinated four-year-old female, with newly diagnosed asplenia in the setting of heterotaxy, had a prolonged hospital course with disseminated haemophilus influenzae type a infection. | 2017 | 28858039 |