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occurrence of osteochondromatosis (multiple cartilaginous exostoses) in a domestic pig (sus scrofa domesticus).osteochondromatosis is a condition in which multiple benign, cartilage-capped tumors arise from the surface of bones formed by endochondral ossification. the current report describes the presence of 4 prominent exophytic masses, measuring between 4 and 13 cm in diameter, arising from the surface of the ribs, and located within the thoracic cavity, in a 2-year-old female domestic pig (sus scrofa domesticus). histological studies revealed that masses were well-differentiated, cartilage-capped prol ...201323847091
assessment of postoperative analgesia after application of ultrasound-guided regional anesthesia for surgery in a swine femoral fracture model.management of pain in research swine used for studies involving painful procedures is a considerable challenge. here we assessed whether a regional anesthesia method is effective for pain control of hindlimb injuries in pigs used for research in bone fracture healing. for this randomized controlled study, we administered regional anesthesia before an experimental femoral injury was produced. using ultrasound guidance, we placed sterile infusion catheters near the sciatic and femoral nerves and a ...023849409
spargana in a weasel, mustela sibirica manchurica, and a wild boar, sus scrofa, from gangwon-do, korea.to know the status of sparganum (plerocercoid of spirometra erinacei) infection in the korean wild life, several species of wild animals were captured in gangwon-do and examined for their status of infection with spargana. from february to december 2011, a total of 62 wild boars, 5 badgers, 1 weasel, 1 siberian chipmunk, and 53 wild rodents were captured, and their whole muscles were examined with naked eyes for the presence of spargana worms. from the weasel and 1 wild boar, a total of 5 sparga ...201323864753
a comparison between rib fracture patterns in peri- and post-mortem compressive injury in a piglet model.forensic biomechanics is increasingly being used to explain how observed injuries occur. we studied infant rib fractures from a biomechanical and morphological perspective using a porcine model.201423867291
adhesion of human and animal escherichia coli strains in association with their virulence-associated genes and phylogenetic origins.intestinal colonization is influenced by the ability of the bacterium to inhabit a niche, which is based on the expression of colonization factors. escherichia coli carries a broad range of virulence-associated genes (vags) which contribute to intestinal (invags) and extraintestinal (exvags) infection. moreover, initial evidence indicates that invags and exvags support intestinal colonization. we developed new screening tools to genotypically and phenotypically characterize e. coli isolates orig ...201323872574
dynamics of ninhydrin-reactive nitrogen and ph in gravesoil during the extended postmortem interval.estimating the postmortem interval of skeletal remains is difficult, as few tools exist to do so. to address this problem, we conducted a field experiment to measure the chemistry of swine (sus scrofa domesticus linnaeus) gravesoil after 1 year and 3 years postmortem. carcasses were placed on the soil surface of a pasture during june in a cold (dfa) climate. significant (p < 0.05) increases in ninhydrin-reactive nitrogen, ph, total nitrogen, and nitrate in gravesoil were detected 1 year postmort ...201323879466
comparative radiographic and resonance frequency analyses of the peri-implant tissue after dental implants placement using flap and flapless techniques: an experimental study on domestic pigs.flapless implant surgery has become very important issue during recent years, mostly thanks to computerization of dentistry and software planning of dental implants placements. the aim of this study was to compare flap and flapless surgical techniques for implant placement through radiographic and radiofrequency analyses.201323885526
evaluation of experimental myocardial infarction models via electromechanical mapping and magnetic resonance imaging.the diagnostic characteristics of electromechanical mapping (emm) were evaluated in porcine myocardial infarction (mi) models with the parallel application of cardiac magnetic resonance imaging (cmri) from the aspect of different pathophysiology and localization. balloon occlusion in the left anterior descending coronary artery (lad balloon group) or coil deployment in the lad (lad coil group) or circumflex artery (cx coil group) was applied percutaneously in 16 domestic pigs. regional left vent ...201323888899
the effect of soil texture on the degradation of textiles associated with buried bodies.there are many factors which affect the rate of decomposition in a grave site including; the depth of burial, climatic conditions, physical conditions of the soil (e.g. texture, ph, moisture), and method of burial (e.g. clothing, wrappings). clothing is often studied as a factor that can slow the rate of soft tissue decomposition. in contrast, the effect of soft tissue decomposition on the rate of textile degradation is usually reported as anecdotal evidence rather than being studied under contr ...201323890657
molecular tracing of classical swine fever viruses isolated from wild boars and pigs in france from 2002 to 2011.there were three outbreaks of classical swine fever (csf) in north-eastern france between 2002 and 2011. the first two occurred in april 2002 in the moselle department, in a wild boar and pig herd, respectively, while the third occurred in april 2003, in the bas-rhin department, in a wild boar. a survey was subsequently implemented in wild boar and domestic pig populations, during which 43 csf viruses (csfvs) were genetically characterized to provide information on virus sources, trace virus evo ...201323891170
the community of hymenoptera parasitizing necrophagous diptera in an urban biotope.most reports published in the field of forensic entomology are focused on diptera and neglect the hymenoptera community. however, hymenoptera are part of the entomofaunal colonization of a dead body. the use of hymenoptera parasitoids in forensic entomology can be relevant to evaluate the time of death. hymenoptera parasitoids of the larvae and pupae of flies may play an important role in the estimation of the post-mortem period because their time of attack is often restricted to a small, well-d ...023895458
molecular characterization and phylogenetic study of african swine fever virus isolates from recent outbreaks in uganda (2010-2013).african swine fever (asf) is a highly lethal and economically significant disease of domestic pigs in eastern africa particularly in uganda where outbreaks regularly occur. sequence analysis of variable genome regions have been extensively used for molecular epidemiological studies of african swine fever virus (asfv) isolates. by combining p72, p54 and pb602l (cvr), a high level resolution approach is achieved for viral discrimination. the major aim of this study therefore, was to investigate th ...201323914918
cryptosporidium suis and cryptosporidium scrofarum in eurasian wild boars (sus scrofa) in central europe.from 2011 to 2012, to identify cryptosporidium spp. occurrence in eurasian wild boars (sus scrofa) 29 randomly selected localities (both forest areas and enclosures) across the central european countries of austria, the czech republic, poland, and the slovak republic were investigated. cryptosporidium oocysts were microscopicaly detected in 11 out of 460 faecal samples examined using aniline-carbol-methyl violet staining. sixty-one cryptosporidium infections, including the 11 infections that wer ...201323916060
assessing the risk of african swine fever introduction into the european union by wild boar.the presence of african swine fever (asf) in the caucasus region and russian federation has increased concerns that wild boars may introduce the asf virus into the european union (eu). this study describes a semi-quantitative approach for evaluating the risk of asf introduction into the eu by wild boar movements based on the following risk estimators: the susceptible population of (1) wild boars and (2) domestic pigs in the country of origin; the outbreak density in (3) wild boars and (4) domest ...201523926953
comprehensive phylogenetic reconstructions of african swine fever virus: proposal for a new classification and molecular dating of the virus.african swine fever (asf) is a highly lethal disease of domestic pigs caused by the only known dna arbovirus. it was first described in kenya in 1921 and since then many isolates have been collected worldwide. however, although several phylogenetic studies have been carried out to understand the relationships between the isolates, no molecular dating analyses have been achieved so far. in this paper, comprehensive phylogenetic reconstructions were made using newly generated, publicly available s ...201323936068
new hosts of simplicimonas similis and trichomitus batrachorum identified by 18s ribosomal rna gene sequences.trichomonads are obligate anaerobes generally found in the digestive and genitourinary tract of domestic animals. in this study, four trichomonad isolates were obtained from carabao, dog, and pig hosts using rectal swab. genomic dna was extracted using chelex method and the 18s rrna gene was successfully amplified through novel sets of primers and undergone dna sequencing. aligned isolate sequences together with retrieved 18s rrna gene sequences of known trichomonads were utilized to generate ph ...201323936631
sirt1 inhibits akt2-mediated porcine adipogenesis potentially by direct protein-protein interaction.compared with the rodent, the domestic pig is a much better animal model for studying adipogenesis and obesity-related diseases. currently, the role of akt2 and sirt1 in porcine adipogenesis remains elusive. in this study, we defined the effect of akt2 and sirt1 on porcine preadipocyte lipogenesis and the regulatory mechanism. first, we found that akt2 was widely expressed in porcine various tissues and at high level in adipose tissue. further analysis showed that the expression level of akt2 wa ...201323951196
evidence of natural transmission of group a rotavirus between domestic pigs and wild boars (sus scrofa) in japan.group a rotaviruses (rvas) are a major cause of acute dehydrating diarrhea in infants and young animals worldwide. rvas have also been detected in several wild and zoo animals, indicating wide susceptibility of wild animals. however, the role of wild animals in the infection cycle of rvas is unclear. wild boars are indigenous in many countries in the world. japanese wild boars (sus scrofa leucomystax) have been migrating close to human habitats in japan, indicating the possibility of natural tra ...201323958658
encoding of situations in the vocal repertoire of piglets (sus scrofa): a comparison of discrete and graded classifications.two important questions in bioacoustics are whether vocal repertoires of animals are graded or discrete and how the vocal expressions are linked to the context of emission. here we address these questions in an ungulate species. the vocal repertoire of young domestic pigs, sus scrofa, was quantitatively described based on 1513 calls recorded in 11 situations. we described the acoustic quality of calls with 8 acoustic parameters. based on these parameters, the k-means clustering method showed a p ...201323967251
tissue uptake, distribution, and healing response after delivery of paclitaxel via second-generation iopromide-based balloon coating: a comparison with the first-generation technology in the iliofemoral porcine model.this study sought to evaluate vascular drug uptake, distribution and response of second-generation paclitaxel coated balloon (pcb) (cotavance, medrad interventional, indianola, pennsylvania) and compare it with first-generation technology, containing identical excipient and drug concentration.201323968706
prediction of volume-responsiveness during one-lung ventilation: a comparison of static, volumetric, and dynamic parameters of cardiac preload.the aim of this study was to evaluate the ability of static, volumetric, and dynamic parameters of cardiac preload to predict volume responsiveness during one-lung ventilation (olv).201323972740
the effect of high-fat diet on the composition of the gut microbiota in cloned and non-cloned pigs of lean and obese phenotype.the aim of this study was to investigate the effect of high-far-high-energy diet on cloned and non-cloned domestic pigs of both lean and obese phenotype and to evaluate if the lean cloned pigs had a lower inter-individual variation as compared with non-cloned pigs. the microbiota of colon and terminal ileum was investigated in cloned and non-cloned pigs that received a high-far-high-energy diet with either restricted or ad libitum access to feed, resulting in lean and obese phenotypes, respectiv ...201723974297
detection of porcine circovirus type 2 (pcv2) and porcine reproductive and respiratory syndrome virus (prrsv) antibodies in meat juice samples from polish wild boar (sus scrofa l.).pcv2 and prrsv are two important pathogens of domestic swine. there is considerable evidence that the infection is also present in wild boars. meat juice provides an alternative to serum for antibody testing, and it has been used in testing for many important porcine infectious diseases. samples of brachial muscle were collected from 142 wild boars shot in different regions of poland during the 2006/2007 and 2007/2008 hunting seasons. meat juice harvested from muscle samples was tested using an ...201323974935
use of domesticated pigs by mesolithic hunter-gatherers in northwestern europe.mesolithic populations throughout europe used diverse resource exploitation strategies that focused heavily on collecting and hunting wild prey. between 5500 and 4200 cal bc, agriculturalists migrated into northwestern europe bringing a suite of neolithic technologies including domesticated animals. here we investigate to what extent mesolithic ertebølle communities in northern germany had access to domestic pigs, possibly through contact with neighbouring neolithic agricultural groups. we emplo ...023982268
influence of sclerotomy use on vitreous incarceration in an experimental model of vitrectomized eye.to evaluate the influence of sclerotomy use during vitrectomy (vitreous cutter, illumination probe, or infusion-line entrance) on postoperative vitreous incarceration using an experimental model of vitrectomized eye.201323983452
the impact of breed and tissue compartment on the response of pig macrophages to lipopolysaccharide.the draft genome of the domestic pig (sus scrofa) has recently been published permitting refined analysis of the transcriptome. pig breeds have been reported to differ in their resistance to infectious disease. in this study we examine whether there are corresponding differences in gene expression in innate immune cells201323984833
splitting of a prevalent mycobacterium bovis spoligotype by variable-number tandem-repeat typing reveals high heterogeneity in an evolving clonal group.mycobacterium bovis populations in countries with persistent bovine tuberculosis usually show a prevalent spoligotype with a wide geographical distribution. this study applied mycobacterial interspersed repetitive-unit-variable-number tandem-repeat (miru-vntr) typing to a random panel of 115 m. bovis isolates that are representative of the most frequent spoligotype in the iberian peninsula, sb0121. vntr typing targeted nine loci: etr-a (alias vntr2165), etr-b (vntr2461), etr-d (miru4, vntr580), ...201323985914
bacterial populations associated with early-stage adipocere formation in lacustrine waters.the preservation of soft tissue is a valuable evidence for forensic investigation as it may provide information about the cause and manner of death as well as the time since death. adipocere forms from the conversion of triglycerides in the neutral fats into stable fatty acids producing a solid white product which aids tissue preservation. adipocere will typically form in water-logged grave sites and aquatic environments. documentation on the chemical and microbiological changes that cause adipo ...201423989223
archaeology. european hunter-gatherers dined on domestic pigs. 201323990538
growing cava vein anastomosis: comparison of cross-clamping and suture times using vcs metallic clips, interrupted nonabsorbable, or continuous absorbable suturing techniques.the latest generation in titanium clip application systems, the anastoclip vessel closure system (vcs; lemaitre vascular, burlington, ma), allows surgeons to perform vascular anastomosis more easily and faster than conventional sutures. this system may become the option of choice for vascular reconstruction in pediatric surgery where, as in the case transplant surgery, decreasing vascular occlusion times may influence outcome. the aim of this study was to determine whether vcs metallic clips wou ...201323993110
characterization of microthermal zones induced by fractional radiofrequency using reflectance confocal microscopy: a preliminary study.the microthermal zone (mtz) produced by fractional device have been mostly evaluated through histopathologic analysis. study of ablative type mtz created by fractional device and skin thermal interaction using in vivo reflectance confocal microscopy (rcm) has been rarely reported.201323996648
serological evidence for avian h9n2 influenza virus infections among romanian agriculture workers.in recent years, wild birds have introduced multiple highly pathogenic avian influenza (hpai) h5n1 virus infections in romanian poultry. in 2005 hpai infections were widespread among domestic poultry and anecdotal reports suggested domestic pigs may also have been exposed. we sought to examine evidence for zoonotic influenza infections among romanian agriculture workers. between 2009 and 2010, 363 adult participants were enrolled in a cross-sectional, seroepidemiological study. confined animal f ...201323999337
immediate graft histological assay, post pig's liver transplantation.to describe the vascular and tissue histopathological changes in seven sequential experimental liver transplantations in pigs.201324000062
n-linked glycan profiling of ggta1/cmah knockout pigs identifies new potential carbohydrate xenoantigens.the temporary or long-term xenotransplantation of pig organs into people would save thousands of lives each year if not for the robust human antibody response to pig carbohydrates. genetically engineered pigs deficient in galactose α1,3 galactose (gene modified: ggta1) and n-glycolylneuraminic acid (gene modified: cmah) have significantly improved cell survival when challenged by human antibody and complement in vitro. there remains, however, a significant portion of human antibody binding.201624033743
emergence of h3n2pm-like and novel reassortant h3n1 swine viruses possessing segments derived from the a (h1n1)pdm09 influenza virus, korea.human-to-swine transmission of the pandemic h1n1 2009 [a(h1n1)pdm09] virus in pig populations resulted in reassortment events with endemic swine influenza viruses worldwide.201324034626
confirmation of intraosseous cannula placement based on pressure measured at the cannula during squeezing the extremity in a piglet model.we sought to assess the reliability of the method using the pressure measured at the intraosseous (io) cannula while squeezing the involved limb (p(squeezing)) in determining the position of the io needle and to compare its performance with that of the traditional confirmation method.201424036195
scaffold-based delivery of autologous mesenchymal stem cells for mandibular distraction osteogenesis: preliminary studies in a porcine model.bone regeneration through distraction osteogenesis (do) is promising but remarkably slow. to accelerate it, autologous mesenchymal stem cells have been directly injected to the distraction site in a few recent studies. compared to direct injection, a scaffold-based method can provide earlier cell delivery with potentially better controlled cell distribution and retention. this pilot project investigated a scaffold-based cell-delivery approach in a porcine mandibular do model.201324040314
comparative histology and immunohistochemistry of porcine versus human skin.porcine skin is increasingly being employed as a model of human skin in various research fields, including pharmacology, toxicology and immunology, with particular interest in percutaneous permeation and organ transplantation. porcine skin shows several anatomical and physiological similarities, but also some differences, with human skin, but few in depth comparative studies are so far available.201324047577
spatial and temporal interactions between livestock and wildlife in south central spain assessed by camera traps.the diversification of livestock farms into hunting estates in south central spain (scs) may impede the success of mycobacterium bovis eradication programmes by facilitating transmission between wildlife and livestock. in this observational study we aimed to provide information of relevance about the nature and frequency of interactions (observed visits to study points) between livestock (cattle and domestic pigs) and wildlife (wild boar and red deer). the study was conducted in an extensive cat ...201324050782
characterization in vitro and in vivo of a novel porcine parainfluenza virus 5 isolate in korea.a novel porcine parainfluenza 5 (ppiv5), knu-11, in the genus rubulavirus of the subfamily paramyxovirinae, was isolated from the lung of a piglet in korea in 2011. to understand the importance of this virus as an infectious agent, in vitro and in vivo characteristics of knu-11 virus was investigated. knu-11 was remarkably cytopathogenic, showing distinct cell rounding and clumping evident in porcine alveolar macrophage (pam), porcine kidney (pk-15), and swine testicle (st) cells within 12h post ...201324050998
extrinsic primary afferent neurons projecting to the pylorus in the domestic pig--localization and neurochemical characteristics.the pig, as an omnivorous animal, seems to be especially valuable species in "gastrointestinal" experiments. the importance of the pylorus in the proper functioning of the digestive tract is widely accepted. although it is commonly known that sensory innervation plays an important role in the regulation of gastric activity and gastrointestinal tissue resistance, there is complete lack of data on the extrinsic afferents projecting to the swine pylorus. the present experiment has been designed to ...201424052481
chimeric anti-cd14 igg2/4 hybrid antibodies for therapeutic intervention in pig and human models of inflammation.cd14 is a key recognition molecule of innate immune responses, interacting with several tlrs. tlr signaling cross-talks extensively with the complement system, and combined cd14 and complement inhibition has been proved effective in attenuating inflammatory responses. pig models of human diseases have emerged as valuable tools to study therapeutic intervention, but suitable neutralizing abs are rare. undesired fc-mediated functions, such as platelet activation and il-8 release induced by the por ...201324062486
radiologic advantages of potential use of polymer plastic clips in neurosurgery.plastic clips are made of diamagnetic material and may result in fewer computed tomography (ct) and magnetic resonance artifacts than titanium clips. considering that polymer plastic clips are increasingly being used in endoscopic surgery, our study examined the ct and magnetic resonance imaging (mri) characteristics of plastic clips after application in the neurocranium and compared them with titanium clips.201624067738
morphological features of the porcine lacrimal gland and its compatibility for human lacrimal gland xenografting.in this study, we present first data concerning the anatomical structure, blood supply and location of the lacrimal gland of the pig. our data indicate that the porcine lacrimal gland may serve as a potential xenograft candidate in humans or as an animal model for engineering of a bioartificial lacrimal gland tissue construct for clinical application. for this purpose, we used different macroscopic preparation techniques and digital reconstruction of the histological gland morphology to gain new ...201324069265
pathogenesis of highly virulent african swine fever virus in domestic pigs exposed via intraoropharyngeal, intranasopharyngeal, and intramuscular inoculation, and by direct contact with infected pigs.to investigate the pathogenesis of african swine fever virus (asfv), domestic pigs (n=18) were challenged with a range (10(2)-10(6) 50% hemadsorbing doses (had50)) of the highly virulent asfv-malawi strain by inoculation via the intraoropharyngeal (iop), intranasopharyngeal (inp), or intramuscular (im) routes. a subsequent contact challenge experiment was performed in which six iop-inoculated donor pigs were allowed to have direct contact (dc) with six naïve pigs for exposure times that varied f ...201324076499
fatal outbreak of systemic pasteurellosis in a wild boar (sus scrofa) population from southwest spain.pasteurella multocida is a common pathogen of swine that causes specific diseases with great economic impact. however, the importance of this pathogen in wild boar is still unknown. in the current work, an outbreak of systemic pasteurellosis in wild boar with a high mortality rate is described. a total of 23 wild boar of all ages were found dead over a 5-day period on a game estate in southwest spain (11.11% mortality). three animals were necropsied and showed subcutaneous edema, a generalized c ...201324081934
modeling of the renal kinetics of the at1 receptor specific pet radioligand [11c]kr31173.the radioligand [(11)c]kr31173 has been introduced for pet imaging of the angiotensin ii subtype 1 receptor (at1r). the purpose of the present project was to employ and validate a compartmental model for quantification of the kinetics of this radioligand in a porcine model of renal ischemia followed by reperfusion (ir).201324083243
mycobacterium bovis infection in domestic pigs in great britain.mycobacterium bovis, the causative agent of bovine tuberculosis (tb), infects a wide range of wild and domestic mammals. despite a control programme spanning decades, m. bovis infection levels in cattle in great britain (gb) have continued to rise over recent years. as the incidence of infection in cattle and wildlife may be linked to that in swine, data relating to infection of pigs identified at slaughter were examined in this study. between 2007 and 2011, almost all m. bovis-infected pigs ori ...201324095608
testis morphometry and kinetics of spermatogenesis in the feral pig (sus scrofa).the feral pig (sus scrofa sp) also known as monteiro pig, originated from a domestic pig breed that was introduced into pantanal region in brazil in the eighteenth century. although the feral pig has commercial potential, there are few reports in the literature concerning the reproductive biology of this species. therefore, the aim of this study was to further describe the feral pig testis parenchyma as well as characterize the stages of the seminiferous epithelium cycle by tubular morphology me ...201324100067
cerebral blood flow quantification in swine using pseudo-continuous arterial spin labeling.to develop quantitative cerebral blood flow (cbf) imaging using pseudo-continuous arterial spin labeling (pcasl) in swine, accounting for their cerebrovascular anatomy and physiology.201324105693
characterization of the envelope glycoprotein of a novel filovirus, lloviu virus.lloviu virus (llov), a novel filovirus detected in bats, is phylogenetically distinct from viruses in the genera ebolavirus and marburgvirus in the family filoviridae. while filoviruses are known to cause severe hemorrhagic fever in humans and/or nonhuman primates, llov is biologically uncharacterized, since infectious llov has never been isolated. to examine the properties of llov, we characterized its envelope glycoprotein (gp), which likely plays a key role in viral tropism and pathogenicity. ...201324131711
comparison of cold crystalloid and colloid infusions for induction of therapeutic hypothermia in a porcine model of cardiac arrest.large-volume cold intravenous infusion of crystalloids has been used for induction of therapeutic hypothermia after cardiac arrest. however, the effectiveness of cold colloids has not been evaluated. therefore, we performed an experimental study to investigate the cooling effect of cold normal saline compared to colloid solution in a porcine model of ventricular fibrillation.201324131867
utility of 64-msct in assessing acute non-reperfused myocardial infarct size.to evaluate the utility of multi-slice computed tomography (msct) in assessing acute non-reperfused myocardial infarct size.201324133512
prolonged therapeutic hypothermia is more effective in attenuating brain apoptosis in a swine cardiac arrest model.to investigate whether 48 hours of therapeutic hypothermia is more effective to attenuate brain apoptosis than 24 hours and to determine whether the antiapoptotic effects of therapeutic hypothermia are associated with the suppressions of the cleavage of protein kinase c-δ, the cytosolic release of cytochrome c, and the cleavage of caspase 3 in a swine cardiac arrest model.201424145844
performance of a novel bipolar/monopolar radiofrequency ablation device on the beating heart in an acute porcine model.although the advent of ablation technology has simplified and shortened surgery for atrial fibrillation, only bipolar clamps have reliably been able to create transmural lesions on the beating heart. currently, there are no devices capable of reproducibly creating the long linear lesions in the right and left atria needed to perform a cox-maze procedure. this study evaluated the performance of a novel suction-assisted radiofrequency device that uses both bipolar and monopolar energy to create le ...201724145972
tissue discrimination by uncorrected autofluorescence spectra: a proof-of-principle study for tissue-specific laser surgery.laser surgery provides a number of advantages over conventional surgery. however, it implies large risks for sensitive tissue structures due to its characteristic non-tissue-specific ablation. the present study investigates the discrimination of nine different ex vivo tissue types by using uncorrected (raw) autofluorescence spectra for the development of a remote feedback control system for tissue-selective laser surgery. autofluorescence spectra (excitation wavelength 377 ± 50 nm) were measured ...201324152930
mechanical ventilation during cardiopulmonary resuscitation with intermittent positive-pressure ventilation, bilevel ventilation, or chest compression synchronized ventilation in a pig model.mechanical ventilation with an automated ventilator is recommended during cardiopulmonary resuscitation with a secured airway. we investigated the influence of intermittent positive-pressure ventilation, bilevel ventilation, and the novel ventilator mode chest compression synchronized ventilation, a pressure-controlled ventilation triggered by each chest compression, on gas exchange, hemodynamics, and return of spontaneous circulation in a pig model.201424158168
microvascular perfusion as a target for fluid resuscitation in experimental circulatory shock.to study regional perfusion during experimental endotoxemic and obstructive shock and compare the effect of initial cardiac output-targeted fluid resuscitation with optimal cardiac output-targeted resuscitation on different peripheral tissues.201424158169
phylogenetic distinction of inos and ido function in mesenchymal stem cell-mediated immunosuppression in mammalian species.mammalian mesenchymal stem cells (mscs) have been shown to be strongly immunosuppressive in both animal disease models and human clinical trials. we have reported that the key molecule mediating immunosuppression by mscs is species dependent: indoleamine 2,3-dioxygenase (ido) in human and inducible nitric oxide synthase (inos) in mouse. in the present study, we isolated mscs from several mammalian species, each of a different genus, and investigated the involvement of ido and inos during msc-med ...201324162664
genomic analyses identify distinct patterns of selection in domesticated pigs and tibetan wild boars.we report the sequencing at 131× coverage, de novo assembly and analyses of the genome of a female tibetan wild boar. we also resequenced the whole genomes of 30 tibetan wild boars from six major distributed locations and 18 geographically related pigs in china. we characterized genetic diversity, population structure and patterns of evolution. we searched for genomic regions under selection, which includes genes that are involved in hypoxia, olfaction, energy metabolism and drug response. compa ...201324162736
direct contact and environmental contaminations are responsible for hev transmission in pigs.hepatitis e virus (hev) can cause enterically-transmitted hepatitis in humans. the zoonotic nature of hepatitis e infections has been established in industrialized areas and domestic pigs are considered as the main reservoir. the dynamics of transmission in pig herds therefore needs to be understood to reduce the prevalence of viremic pigs at slaughter and prevent contaminated pig products from entering the food chain. an experimental trial was carried out to study the main characteristics of he ...201324165278
beta radiation for renal nerve denervation: initial feasibility and safety.in small clinical trials, sympathetic renal denervation using radiofrequency (rf) energy shows promise in treating resistant hypertension. however, the rf procedure is lengthy and is associated with pain during ablation. vascular brachytherapy, a proven treatment for in-stent restenosis, has the potential to cause nerve fibrosis. the purpose of the present study was to assess the safety and feasibility of renal artery brachytherapy for sympathetic renal denervation.201324169134
conservation genomic analysis of domestic and wild pig populations from the iberian peninsula.inbreeding is among the major concerns in management of local livestock populations. the effective population size of these populations tends to be small, which enhances the risk of fitness reduction and extinction. high-density snp data make it possible to undertake novel approaches in conservation genetics of endangered breeds and wild populations.a total of 97 representative samples of domestic and wild pig populations from the iberian peninsula, subjected to different levels of threat with e ...201324172017
ancient dna and population turnover in southern levantine pigs--signature of the sea peoples migration?near eastern wild boars possess a characteristic dna signature. unexpectedly, wild boars from israel have the dna sequences of european wild boars and domestic pigs. to understand how this anomaly evolved, we sequenced dna from ancient and modern pigs from israel. pigs from late bronze age (until ca. 1150 bce) in israel shared haplotypes of modern and ancient near eastern pigs. european haplotypes became dominant only during the iron age (ca. 900 bce). this raises the possibility that european p ...201324186332
adhesion patterns of commensal and pathogenic escherichia coli from humans and wild animals on human and porcine epithelial cell lines.different strategies of colonization or infection by e. coli result in formation of certain adhesion patterns which help also in classifying intestinal e. coli into pathotypes. little is known about adhesion patterns and host- and tissue adaption of commensal e. coli and about e. coli originating in clinically healthy hosts carrying pathotype-specific virulence-associated genes.201324188314
the hippocampus of the eastern rock sengi: cytoarchitecture, markers of neuronal function, principal cell numbers, and adult neurogenesis.the brains of sengis (elephant shrews, order macroscelidae) have long been known to contain a hippocampus that in terms of allometric progression indices is larger than that of most primates and equal in size to that of humans. in this report, we provide descriptions of hippocampal cytoarchitecture in the eastern rock sengi (elephantulus myurus), of the distributions of hippocampal calretinin, calbindin, parvalbumin, and somatostatin, of principal neuron numbers, and of cell numbers related to p ...201324194702
the expression of mitochondrial, cytoplasmic and extracellular superoxide dismutase in the colonic wall of pigs suffering from swine dysenteria.the expression of 3 types of peroxide dismutase (sod1, sod2 and sod3) was studied with real-time pcr in the colonic wall of domestic pig suffering from swine dysentery. the expression of enzymes was studied separately in the mucosa and the muscular membrane. it was found that in the mucosa the expression of sod1 (cytoplasmic) did not change, while the levels of expression of mitochondrial sod2 and extracellular sod3 were raised in inflamed colon. more dramatic changes were seen in the muscular m ...201324195279
juvenile domestic pigs (sus scrofa domestica) use human-given cues in an object choice task.research on the comprehension of human-given cues by domesticated as well as non-domesticated species has received considerable attention over the last decade. while several species seem to be capable of utilizing these cues, former work with domestic pigs (sus scrofa domestica) has shown inconclusive results. in this study, we investigated the use of human-given cues in an object choice task by young domestic pigs (n = 17; 7 weeks of age) who had very limited human contact prior to the experime ...201424197275
sperm nuclear architecture is locally modified in presence of a robertsonian translocation t(13;17).in mammals, the non-random organization of the sperm nucleus supports an early function during embryonic development. altering this organization may interfere with the zygote development and reduce fertility or prolificity. thus, rare studies on sperm cells from infertile patients described an altered nuclear organization that may be a cause or a consequence of their respective pathologies. thereby, chromosomal rearrangements and aneuploidy can be studied not only for their adverse effects on pr ...201324205066
the challenges of classical swine fever control: modified live and e2 subunit vaccines.classical swine fever (csf) is an economically important, highly contagious disease of swine worldwide. csf is caused by classical swine fever virus (csfv), and domestic pigs and wild boars are its only natural hosts. the two main strategies used to control csf epidemic are systematic prophylactic vaccination and a non-vaccination stamping-out policy. this review compares the protective efficacy of the routinely used modified live vaccine (mlv) and e2 subunit vaccines and summarizes the factors ...201424211665
epidemiological survey of swine influenza a virus in the wild boar population of two italian provinces.an epidemiological survey was carried out in order to obtain a better understanding of the role of wild boars in the epidemiology of the influenza virus.201324224815
flaviviruses in europe: complex circulation patterns and their consequences for the diagnosis and control of west nile disease.in europe, many flaviviruses are endemic (west nile, usutu, tick-borne encephalitis viruses) or occasionally imported (dengue, yellow fever viruses). due to the temporal and geographical co-circulation of flaviviruses in europe, flavivirus differentiation by diagnostic tests is crucial in the adaptation of surveillance and control efforts. serological diagnosis of flavivirus infections is complicated by the antigenic similarities among the flavivirus genus. indeed, most flavivirus antibodies are ...201324225644
experimental infection of collared peccary ( dicotyles tajacu angulatus ) with swine kidney worm ( stephanurus dentatus ).two captive-born juvenile collared peccaries ( dicotyles tajacu angulatus ) were given 3000 infective larvae of stephanurus dentatus per os. one peccary harbored viable s. dentatus sub-adults in the liver 50 days post-infection. the other peccary had no larvae but did have diffuse fibrotic hepatic lesions and bile duct hyperplasia 213 days post-infection; however, the lesions may have been partially due to a concurrent ascaris suum infection. a domestic pig ( sus scrofa domesticus ) infected as ...197724228971
post-burning fragmentation of calcined bone: implications for remains recovery from fatal fire scenes.this research assesses how short term delays in time-until-recovery can affect the quality and quantity of burnt bone recovered from a fatal fire scene. knowledge of trends in post-burning remains fragmentation will enable investigators to prioritise remains recovery and implement recovery protocols appropriately. by comparing calcined bone fragments recovered 0, 24, 56 and 168 h (1 week) after experimental burns, this research describes remains fragmentation over time. sus scrofa (domestic pig) ...201324237832
dental implant macro-design features can impact the dynamics of osseointegration.the purpose of this study was to compare the clinical performance of two dental implant types possessing a different macro-design in the in vivo pig model.201524238310
genomic analysis of influenza a virus from captive wild boars in brazil reveals a human-like h1n2 influenza virus.influenza is a viral disease that affects human and several animal species. in brazil, h1n1, h3n2 and 2009 pandemic h1n1 a(h1n1)pdm09 influenza a viruses (iav) circulate in domestic swine herds. wild boars are also susceptible to iav infection but in brazil until this moment there are no reports of iav infection in wild boars or in captive wild boars populations. herein the occurrence of iav in captive wild boars with the presence of lung consolidation lesions during slaughter was investigated. ...201424238665
age-related and regional differences in the prevalence of hepatitis e virus-specific antibodies in pigs in germany.an increasing number of acute autochthonous human hepatitis e virus (hev)-infections was noticed in germany and other developed countries, most likely the result of a zoonotic virus transmission from pig, wild boar and deer. currently there is still a lack of profound data concerning the actual prevalence of hev-specific antibodies in domestic pig herds in germany, in particular for regions with high pig density, and its age-dependency. 2273 domestic pig sera were collected in 2011 mainly from b ...201324238666
pheromonal transmission of an aversive experience in domestic pig.the process of spontaneous learning in an automatic food dispenser by a group of domestic female pigs was studied when one of the animals of the group had an aversive experience. restraining a gilt in the dispenser without access to food resulted in later avoidance of the system by other gilts, especially when the reactions of the restrained animal had been especially violent and associated with urination. the hypothesis of a delayed transmission of an unpleasant experience was tested. the food ...199224254286
an integrated pipeline for next generation sequencing and annotation of the complete mitochondrial genome of the giant intestinal fluke, fasciolopsis buski (lankester, 1857) looss, 1899.helminths include both parasitic nematodes (roundworms) and platyhelminths (trematode and cestode flatworms) that are abundant, and are of clinical importance. the genetic characterization of parasitic flatworms using advanced molecular tools is central to the diagnosis and control of infections. although the nuclear genome houses suitable genetic markers (e.g., in ribosomal (r) dna) for species identification and molecular characterization, the mitochondrial (mt) genome consistently provides a ...201324255820
an overview of the host spectrum and distribution of calodium hepaticum (syn. capillaria hepatica): part 2-mammalia (excluding muroidea).calodium hepaticum (syn. capillaria hepatica) is a globally distributed zoonotic nematode with low host specificity and a high affinity to the liver. although murid rodents are the main definite hosts, various other mammals can be affected with hepatic capillariasis: non-murid rodents, insectivora, chiroptera, lagomorpha, artiodactyla, perissodactyla, hyracoidea, marsupialia, carnivora, and primates. overall, more than 180 mammalian species (including humans) are known as suitable hosts of this ...201324257974
spatio-temporal analysis of the progression of aujeszky's disease virus infection in wild boar of saxony-anhalt, germany.aujeszky's disease (ad, pseudorabies) is a notifiable disease caused by suid herpesvirus 1 (suhv-1), also named pseudorabies virus (prv). the study aimed at determining the occurrence and spatio-temporal trend of specific antibodies to ad virus (adv) among wild boar of saxony-anhalt, a landlocked federal state situated in the western part of eastern germany. to this end, a total of 7,209 blood samples were collected and tested from 2000 to 2011. an average seroprevalence of 6.8% was found for th ...201324258896
acute inflammatory response to transgastric natural orifice transluminal endoscopic surgery peritoneoscopy: an experimental study in swine.to investigate the impact of transgastric peritoneal access on plasma biomarkers of acute inflammatory response in comparison to laparoscopy.024270956
porcine hokovirus in domestic pigs, cameroon. 201324274159
synthesis of α-collagen fragments and research of their influence on the degree of hydration of a model of epidermis.in recent years the interest into areas of science, such as cosmetology, dermatology, pharmacology or aesthetic medicine has increased significantly. scientists are more frequently looking for ingredients that affect the skin's condition and slow down the aging process. practically every year, the scientists discover a number of new chemical substances (both natural and synthetic) that can be potentially used to manufacture cosmetics.201324278040
transcriptome profiling identifies differentially expressed genes in postnatal developing pituitary gland of miniature pig.in recent years, tibetan pig and bama pig are popularly used as animal models for medical researches. however, little genomic information is available for the two breeds, particularly regarding gene expression pattern at the whole-transcriptome level. in this study, we characterized the pituitary transcriptome profile along their postnatal developmental stages within and between the two breeds in order to illustrate the differential dynamics and functions of differentially expressed genes. we ob ...201324282060
interdisciplinary collaborative team for blastocyst implantation research: inception and perspectives. 201324286196
prevalence and diversity of encephalitozoon spp. and enterocytozoon bieneusi in wild boars (sus scrofa) in central europe.from 2011 to 2012, the occurrence of enterocytozoon bieneusi and encephalitozoon spp. was surveyed at 29 randomly selected localities (both forest areas and enclosures) across four central european countries: austria, the czech republic, poland, and the slovak republic. isolates were genotyped by pcr amplification and characterization of the internal transcribed spacer (its) region using enterocytozoon and encephalitozoon-specific protocols. pcr revealed 16 mono-infections of encephalitozoon cun ...201424292543
detection and molecular characterization of porcine picobirnavirus in feces of domestic pigs from kolkata, india.picobirnaviruses (pbvs) are small, non-enveloped, 35-41 nm virion with bisegmented double-stranded rna genome. pbvs are widespread and were detected in feces of humans and a wide variety of animals. domestic pig, one of the ubiquitous farm animal reported incessant association with a variety of viral zoonoses. the objective of our study is to find out the incidence of pbv infection in healthy domestic pigs. the study was conducted by collecting feces of healthy/asymptomatic pigs from a piggery l ...201224293831
endocrine rhythms in the brown bear (ursus arctos): evidence supporting selection for decreased pineal gland size.many temperate zone animals adapt to seasonal changes by altering their physiology. this is mediated in large part by endocrine signals that encode day length and regulate energy balance and metabolism. the objectives of this study were to determine if the daily patterns of two important hormones, melatonin and cortisol, varied with day length in captive brown bears (ursus arctos) under anesthetized and nonanesthetized conditions during the active (march-october) and hibernation periods. melaton ...201324303132
therapeutic safety of high myocardial expression levels of the molecular inotrope s100a1 in a preclinical heart failure model.low levels of the molecular inotrope s100a1 are sufficient to rescue post-ischemic heart failure (hf). as a prerequisite to clinical application and to determine the safety of myocardial s100a1 dna-based therapy, we investigated the effects of high myocardial s100a1 expression levels on the cardiac contractile function and occurrence of arrhythmia in a preclinical large animal hf model. at 2 weeks after myocardial infarction domestic pigs presented significant left ventricular (lv) contractile d ...201424305416
introduction of two chromosomal translocations of sus scrofa nigripes and sus scrofa scrofa into the genome of sus scrofa domestica.hybrids between wild and domestic pigs with two types of translocations in the karyotype were studied. the translocations of type i were first detected in a population of the middle asian wild boars. type ii was identified in a populations of the central european subspecies. a large number of middle asian and central european hybrids and their f1-f3 hybrids from crosses with domestic pigs were viable. by means of differential chromosome staining, the mechanism of the formation of 'synthetic' kar ...197824309902
transumbilical endoscopic cholecystectomy in a porcine model.natural orifice transluminal endoscopic surgery (notes) is a new technique. this study describes our initial experience of notes and investigates the feasibility of transumbilical endoscopic cholecystectomy (tuec).201324316742
case report: pig attack with successful postexposure prophylaxis management.the domesticated pig or boar is ubiquitous to agriculture around the world and is an important food product in most countries. the males that are able to grow tusks that can approach 5 in are the primary contributor to morbidity and mortality from boar attacks. literature surrounding domesticated boar attacks is sparse. there are no published cases of domesticated boar attacks that describe successful bacterial postexposure prophylaxis. we present a case report of an attack on an adult female by ...201424332899
influence of hypotension and nerve root section on the ability to mobilize the spinal cord during spine surgery. an experimental study in a pig model.the correction of severe spinal deformities by an isolated posterior approach often involves cord manipulation together with hypotensive anesthesia. to date, the efficiency of methods to increase the tolerance of the cord to displacement and the influence of hypotension on this tolerance is yet to be assessed.201424333455
dynamic changes in nuclear import of a nuclear localisation signal-bearing substrate in 8-cell stage porcine embryos.coordinated intracellular trafficking is critically important for proper timing of major cellular events during embryogenesis. nuclear import mediated by the karyopherin α/β (importin α/β) heterodimer is perhaps the best characterised nuclear trafficking system in eukaryotic cells. seven karyopherin α subtypes have been identified in the domestic pig, and although each karyopherin α subtype transports proteins bearing classical nuclear localisation signals (nlss), individual karyopherin α subtyp ...201524342392
expression and genomic imprinting of the porcine rasgrf1 gene.imprinted genes play important roles in mammalian growth, development and behavior. the rasgrf1 (ras protein-specific guanine nucleotide exchange factor 1) gene has been identified as an imprinted gene in mouse and rat. in the present study, we detected its sequence, imprinting status and expression pattern in the domestic pigs. a 228 bp partial sequence located in exon 14 and a 193 bp partial sequence located in exon 1 of the rasgrf1 gene in domestic pigs were obtained. a g/a transition, was id ...201424342659
infrared skin damage thresholds from 1319-nm continuous-wave laser exposures.a series of experiments were conducted in vivo using yucatan miniature pigs (sus scrofa domestica) to determine thermal damage thresholds to the skin from 1319-nm continuous-wave nd:yag laser irradiation. experiments employed exposure durations of 0.25, 1.0, 2.5, and 10 s and beam diameters of ∼0.6 and 1 cm. thermal imagery data provided a time-dependent surface temperature response from the laser. a damage endpoint of fifty percent probability of a minimally visible effect was used to determine ...201324343435
cardiac expression and atrial fibrillation-associated remodeling of k₂p2.1 (trek-1) k⁺ channels in a porcine model.effective management of atrial fibrillation (af) often remains an unmet need. cardiac two-pore-domain k(+) (k2p) channels are implicated in action potential regulation, and their inhibition has been proposed as a novel antiarrhythmic strategy. k2p2.1 (trek-1) channels are expressed in the human heart. this study was designed to identify and functionally express porcine k2p2.1 channels. in addition, we sought to analyze cardiac expression and af-associated k2p2.1 remodeling in a clinically releva ...201424345461
[influence of the depth of retained denatured dermis on the survival rate of grafted skin in burn swine with deep partial-thickness burn].to explore the influence of the thickness of retained denatured dermis on the survival rate of grafted skin in swine with deep partial-thickness burn.201324351536
molecular detection and genetic analysis of porcine bocavirus in korean domestic swine herds.several porcine bocaviruses have been detected worldwide, and this report is the first to describe this virus in a korean domestic swine herd. we identified porcine bocavirus in various samples, including serum, tissue, feces and saliva, which revealed that porcine bocavirus predominates in korean domestic swine populations. the results of this study also suggested that porcine bocaviruses primarily infect weaned piglets. phylogenetic analysis of the orf3 gene was performed to determine the gene ...201424352435
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