Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| new antitrichomonal drug-like chemicals selected by bond (edge)-based tomocomd-cardd descriptors. | bond-based quadratic indices, new tomocomd-cardd molecular descriptors, and linear discriminant analysis (lda) were used to discover novel lead trichomonacidals. the obtained lda-based quantitative structure-activity relationships (qsar) models, using nonstochastic and stochastic indices, were able to classify correctly 87.91% (87.50%) and 89.01% (84.38%) of the chemicals in training (test) sets, respectively. they showed large matthews correlation coefficients of 0.75 (0.71) and 0.78 (0.65) for ... | 2008 | 18753687 |
| biochemical characterization of a mitochondrial-like organelle from blastocystis sp. subtype 7. | a mitochondrion-like organelle (mlo) was isolated from isotonic homogenates of blastocystis. the organelle sedimented at 5000 g for 10 min, and had an isopycnic density in sucrose of 1.2 g ml(-1). biochemical characterization enabled the demonstration of several key enzymes that allowed the construction of a metabolic pathway consisting of an incomplete krebs cycle linked to the oxygen-sensitive enzymes pyruvate : nadp(+) oxidoreductase (pno), acetate : succinate coa transferase (asct) and succi ... | 2008 | 18757809 |
| co-occurrence of intoxication during sex and sexually transmissible infections among young african american women: does partner intoxication matter? | the co-occurrence of a behaviour (being intoxicated on alcohol/drugs during sex) with a disease outcome [laboratory-confirmed sexually transmissible infection (sti) prevalence] among young african american women and their male sex partners was studied. | 2008 | 18771645 |
| giardiadb and trichdb: integrated genomic resources for the eukaryotic protist pathogens giardia lamblia and trichomonas vaginalis. | giardiadb (http://giardiadb.org) and trichdb (http://trichdb.org) house the genome databases for giardia lamblia and trichomonas vaginalis, respectively, and represent the latest additions to the eupathdb (http://eupathdb.org) family of functional genomic databases. giardiadb and trichdb employ the same framework as other eupathdb sites (cryptodb, plasmodb and toxodb), supporting fully integrated and searchable databases. genomic-scale data available via these resources may be queried based on b ... | 2009 | 18824479 |
| a novel dna sequence periodicity decodes nucleosome positioning. | there have been two types of well-characterized dna sequence periodicities; both are found to be associated with important molecular mechanisms. one is a 3-nt periodicity corresponding to codon triplets, the other is a 10.5-nt periodicity related to the structure of dna helixes. in the process of analyzing the genome and transcriptome of trichomonas vaginalis, we observed a 120.9-nt periodicity along dna sequences. different from the 3- and 10.5-nt periodicities, this novel periodicity originate ... | 2008 | 18829715 |
| topical fenticonazole in dermatology and gynaecology: current role in therapy. | fenticonazole is an imidazole derivative with a broad spectrum of antimycotic activity against dermatophytes and yeasts in in vitro and clinical studies. fenticonazole exerts its unique antimycotic mechanism of action in the following three ways: (i) inhibition of the secretion of protease acid by candida albicans; (ii) damage to the cytoplasmic membrane; and (iii) by blocking cytochrome oxidases and peroxidises. fenticonazole has also been shown to exhibit antibacterial action, with a spectrum ... | 2008 | 18840006 |
| molecular typing of the actin gene of trichomonas vaginalis isolates by pcr-restriction fragment length polymorphism. | human trichomoniasis, caused by the protozoan trichomonas vaginalis, is a highly prevalent sexually transmitted infection. however, little is known about the degree of strain variability of t. vaginalis. a reliable classification method for t. vaginalis strains would be a useful tool in the study of the epidemiology, pathogenesis and transmission of t. vaginalis. a pcr-restriction fragment length polymorphism typing method was designed and evaluated using t. vaginalis isolates obtained after cul ... | 2008 | 18844685 |
| human urinary parasites in nalout popularity, libya. | a total of 2000 fresh urine samples were collected from a central private laboratory in tigi conference which served all the popularity. the examination showed motile trichomonas vaginalis in seven patients (0.35%); five females and two males, and enterobius vermicularis in two female children (0.1%). none had urinary schistosomiasis. the data were discussed. | 2008 | 18853614 |
| trichomonas vaginalis antigens in vaginal and urine specimens by immunochromatography, compared to culture and microscopy. | this study evaluated the immunochromatographic (ic) capillary flow technology for detection of t. vaginalis antigens in vaginal and urine specimens. the ic antigen-detection test and microscopy wet smears were assessed against the inpouch tv culture. vaginal-swabs and first-voided urine specimens were obtained from 348 sexually active symptomatic or asymptomatic, > or =16 years old women. nineteen samples (5%) were positive by culture (95%, ci 0.3-0.8). the ic test (vaginal-specimens) was more s ... | 2008 | 18853629 |
| trichomonas vaginalis; incidence, signs, symptomatology and special cases. | 1948 | 18860737 | |
| in vitro action of various chemical agents on trichomonas hominis, trichomonas vaginalis and trichomonas foetus. | 1948 | 18865906 | |
| treatment of trichomonas vaginalis with tyrothricin. | 1948 | 18869732 | |
| [observations on trichomonas vaginalis vaginitis associated with venereal disease; its treatment]. | 1948 | 18883164 | |
| arsenical poisoning following the use of carbarsone in the treatment of trichomonas vaginalis vaginitis. | 1948 | 18889048 | |
| [gonorrheal infection and trichomonas vaginalis]. | 1948 | 18893810 | |
| protein hydrolysate-enriched media for the propagation of trichomonas vaginalis (donné). | 1948 | 18898703 | |
| the nutrition of protozoa; an improved procedure for separating human blood serum into the two fractions essential for the sustained growth of trichomonas vaginalis. | 1948 | 18902237 | |
| the seventh venereal disease? | 1948 | 18910498 | |
| nutritional requirements of trichomonas vaginalis. | 1948 | 18910616 | |
| chemotherapy of bacteria-free trichomonas vaginalis; action of analogues of pantothenic acid. | 1948 | 18910648 | |
| trichomonas vaginalis infestation in women. | 1948 | 18916389 | |
| persistent urethritis and prostatitis due to trichomonas vaginalis: a case report. | the present report describes a case of persistent urethritis accompanied by prostatitis due to trichomonas vaginalis in a young male patient. the importance of the laboratory diagnosis of trichomoniasis in persistent or recurrent urethritis (ie, testing samples from multiple sites) is highlighted, with the aim of improving the clinical recognition of this pathogen. | 2007 | 18923725 |
| a 25-bp ancient spliceosomal intron in the tvrab1a gene of trichomonas vaginalis. | spliceosomal introns play a key role in eukaryotic genome evolution and protein diversity. a large rab gtpase family has been identified in a unicellular eukaryote trichomonas vaginalis. however, the characteristics of introns in rab genes of t. vaginalis have not been investigated previously. in this study, we identified a 25-bp spliceosomal intron in the t. vaginalis rab1a (tvrab1a) gene, the smallest intron in t. vaginalis to be characterized to date. this intron contains a canonical splice s ... | 2009 | 18926921 |
| prevalence of mycoplasma genitalium in health clinic attendees complaining of vaginal discharge in bangladesh. | the objective of the study was to determine the prevalence of mycoplasma genitalium in a sample of health clinic attendees complaining of vaginal discharge. a subsample of 399 vaginal and cervical swabs was randomly selected from 2579 samples collected during a study to determine the causes of vaginal discharge in women attending primary health-care clinics in dhaka, bangladesh. cervical samples were tested for m. genitalium by polymerase chain reaction. in addition, the samples were tested for ... | 2008 | 18931272 |
| enzymatic assay for total plasma cys. | patients with vascular disease and end-stage renal disease have significantly higher concentrations of plasma total cys (tcys) than do healthy individuals. described here is a nonradioactive, precise, rapid and sensitive enzymatic colorimetric assay for tcys in plasma samples and is homogeneous in that it avoids separation methods. the tcys assay uses only the recombinant enzymes methionine alpha,gamma-lyase (rmetase) and s-adenosylhomocysteine hydrolase (rsahh) cloned from pseudomonas putida an ... | 2008 | 18948977 |
| the divergent eukaryote trichomonas vaginalis has an m7g cap methyltransferase capable of a single n2 methylation. | eukaryotic rnas typically contain 5' cap structures that have been primarily studied in yeast and metazoa. the only known rna cap structure in unicellular protists is the unusual cap4 on trypanosoma brucei mrnas. we have found that t. vaginalis mrnas are protected by a 5' cap structure, however, contrary to that typical for eukaryotes, t. vaginalis spliceosomal snrnas lack a cap and may contain 5' monophophates. the distinctive 2,2,7-trimethylguanosine (tmg) cap structure usually found on snrnas ... | 2008 | 18957443 |
| the effects of male circumcision on female partners' genital tract symptoms and vaginal infections in a randomized trial in rakai, uganda. | the objective of the study was to assess effects of male circumcision on female genital symptoms and vaginal infections. | 2009 | 18976733 |
| differential inhibition of high and low mr thioredoxin reductases of parasites by organotelluriums supports the concept that low mr thioredoxin reductases are good drug targets. | thioredoxin reductase (trxr), a nadph-dependent disulfide oxidoreductase, is vital in numerous cellular processes including defence against reactive oxygen species, cell proliferation and signal transduction. trxrs occur in 2 forms, a high mr enzyme characterized by those of mammals, the malaria parasite plasmodium falciparum and some worms, and a low mr form is present in bacteria, fungi, plants and some protozoan parasites. our hypothesis is that the differences between the forms can be exploi ... | 2009 | 18980703 |
| stepwise diagnosis of trichomonas vaginalis infection in adolescent women. | the objective of this study was to examine the effects of clinical factors and of the type and timing of a secondary test in improving the sensitivity of trichomonas vaginalis detection in young women over that of a wet mount alone. for this purpose, sexually active adolescent women (n = 345) were recruited from a hospital teen clinic or emergency department. following an interview and a pelvic exam, four primary t. vaginalis tests (wet mount, culture, a rapid test, and a nucleic acid amplificat ... | 2009 | 18987174 |
| phase ii clinical trial with praneem polyherbal tablets for assessment of their efficacy in symptomatic women with abnormal vaginal discharge (an icmr task force study). | abnormal vaginal discharge syndrome (avds) is a commonly observed gynaecological complaint for which women seek medical attention. the present study was conducted in six indian council of medical research centres with praneem polyherbal tablets (ppt), to determine their efficacy in the treatment of symptomatic women with avds. data are given on 141 subjects investigated. in total, 137 women (97%) reported complete (n=62, 44%) and partial (n=75, 53%) relief from symptoms after use of ppt for seve ... | 2009 | 18990416 |
| trichomonas vaginalis treatment reduces vaginal hiv-1 shedding. | vaginal hiv-1 shedding has been associated with trichomonas vaginalis (tv) infection and could play a role in hiv transmission. the purpose of the study was to examine if effective tv treatment reduces the presence of vaginal hiv-1 rna. | 2009 | 19008776 |
| flavodiiron protein from trichomonas vaginalis hydrogenosomes: the terminal oxygen reductase. | trichomonas vaginalis is one of a few eukaryotes that have been found to encode several homologues of flavodiiron proteins (fdps). widespread among anaerobic prokaryotes, these proteins are believed to function as oxygen and/or nitric oxide reductases to provide protection against oxidative/nitrosative stresses and host immune responses. one of the t. vaginalis fdp homologues is equipped with a hydrogenosomal targeting sequence and is expressed in the hydrogenosomes, oxygen-sensitive organelles ... | 2009 | 19011120 |
| anti-trichomonas vaginalis activity of synthetic lipophilic diamine and amino alcohol derivatives. | taking in account the increased prevalence of metronidazole-resistant infections, alternative drugs are necessary for the treatment of trichomonosis. we report in this work the preparation and the in vitro anti-trichomonads activity of two diamines 1 and 2, and three different lipophilic amino alcohol derivatives 3, 4 and 5. these compounds were tested for in vitro activity against two isolates of trichomonas vaginalis and displayed inhibition of the parasite growth. five concentrations of each ... | 2009 | 19013752 |
| giardia, entamoeba, and trichomonas enzymes activate metronidazole (nitroreductases) and inactivate metronidazole (nitroimidazole reductases). | infections with giardia lamblia, entamoeba histolytica, and trichomonas vaginalis, which cause diarrhea, dysentery, and vaginitis, respectively, are each treated with metronidazole. here we show that giardia, entamoeba, and trichomonas have oxygen-insensitive nitroreductase (ntr) genes which are homologous to those genes that have nonsense mutations in metronidazole-resistant helicobacter pylori isolates. entamoeba and trichomonas also have nim genes which are homologous to those genes expressed ... | 2009 | 19015349 |
| vulnerability of women in southern africa to infection with hiv: biological determinants and priority health sector interventions. | to review biomedical determinants of women's vulnerability to infection with hiv and interventions to counter this, within the southern african context. | 2008 | 19033753 |
| microbiological and toxicological effects of perla black bean (phaseolus vulgaris l.) extracts: in vitro and in vivo studies. | we investigated the microbiological and toxicological effects of three perla black bean extracts on the growth and culture of selected pathogenic microorganisms, the toxicity over vero cell lines and an in vivo rat model. three different solvents were used to obtain perla black bean extracts. all three perla black bean extracts were tested for antibacterial and antiparasitic activity and further analysed for intrinsic cytotoxicity (ic(50)). methanol perla black bean extract was used for acute to ... | 2009 | 19053992 |
| a proteome reference map of trichomonas vaginalis. | trichomoniasis caused by trichomonas vaginalis is the most common sexual transmitted infection in the world. the 170-mb genome of this protozoan contains 60,000 genes, the largest number of genes ever identified in protozoan. high-throughput expression sequenced tag analysis showed that at least 4,000 genes were expressed in the trophozoite stage. in the present study, we use two-dimensional electrophoresis combined with matrix-assisted laser desorption ionization time-of-flight mass spectrometr ... | 2009 | 19066965 |
| epidemiology of trichomonas vaginalis among women in lagos metropolis, nigeria. | a total of 544 women outpatients comprising 320 and 224 from lagos university teaching hospital (luth), idi-araba, lagos and military hospital, yaba, lagos, respectively, were examined for trichomonas vaginalis infection. both wet mount microscopy and giemsa staining technique were used. a prevalence of 3.3% was recorded from the 544 women using both methods, 5.4% in the military hospital and 1.9% from luth. the difference in prevalence was statistically significant using chi-square test analysi ... | 2007 | 19070181 |
| male circumcision and neisseria gonorrhoeae, chlamydia trachomatis and trichomonas vaginalis: observations after a randomised controlled trial for hiv prevention. | to assess the association between male circumcision and neisseria gonorrhoeae, chlamydia trachomatis and trichomonas vaginalis using data from a male circumcision randomised controlled trial. | 2009 | 19074928 |
| in vitro testing of rationally designed spermicides for selectively targeting human sperm in vagina to ensure safe contraception. | rational synthesis of novel structures resulted in two unique molecules (dse-36 and dse-37, disulphide esters of carbothioic acid) that killed sperm 25 times more strongly and with a precisely targeted action than nonoxynol-9 (n-9). we examine the effects of dse-36 and dse-37 on human spermatozoa versus hela cells to establish specificity and safety compared with n-9. | 2009 | 19088111 |
| reductive evolution of the mitochondrial processing peptidases of the unicellular parasites trichomonas vaginalis and giardia intestinalis. | mitochondrial processing peptidases are heterodimeric enzymes (alpha/betampp) that play an essential role in mitochondrial biogenesis by recognizing and cleaving the targeting presequences of nuclear-encoded mitochondrial proteins. the two subunits are paralogues that probably evolved by duplication of a gene for a monomeric metallopeptidase from the endosymbiotic ancestor of mitochondria. here, we characterize the mpp-like proteins from two important human parasites that contain highly reduced ... | 2008 | 19096520 |
| partial sequence of the beta-tubulin of histomonas meleagridis and the activity of benzimidazoles against h. meleagridis in vitro. | the protozoan parasite histomonas meleagridis is a member of the family monocercomonadidae in the class trichomonada. due to food safety concerns, currently no prophylactic or therapeutic drug against the parasite is licensed in the european union. benzimidazoles are antiparasitic drugs, and some of them are licensed for use in food-producing animals. benzimidazoles act on beta-tubulin, and the beta-tubulin sequence allows predictions about the efficacy of benzimidazoles. in this study, we seque ... | 2009 | 19101732 |
| acetate:succinate coa-transferase in the anaerobic mitochondria of fasciola hepatica. | fasciola hepatica contains anaerobically functioning mitochondria that produce acetate and propionate, the main endproducts excreted by this parasite. the final reactions in the pathways leading to these endproducts are performed by acetate:succinate coa-transferase (asct) and propionate:succinate coa-transferase (psct), respectively. the enzymes catalysing these essential reactions in anaerobic mitochondria are still not characterized, nor are the corresponding genes identified. here we describ ... | 2009 | 19103231 |
| accuracy of the clinical diagnosis of vaginitis compared with a dna probe laboratory standard. | to estimate the accuracy of the clinical diagnosis of the three most common causes of acute vulvovaginal symptoms (bacterial vaginosis, candidiasis vaginitis, and trichomoniasis vaginalis) using a traditional, standardized clinical diagnostic protocol compared with a dna probe laboratory standard. | 2009 | 19104364 |
| trichomonas vaginalis: the adhesins ap51 and ap65 bind heme and hemoglobin. | trichomonas vaginalis is the cause of human trichomoniasis, the most common non-viral sexually transmitted disease worldwide. although acquisition of iron by binding to host hemoglobin through distinct receptor(s) has been described, no specific heme- or hemoglobin-binding site has been reported in this parasite. to determine the presence of hemoglobin-binding protein(s), membrane proteins were subjected to hemoglobin-affinity chromatography. eluted proteins were analysed by sds-page. two protei ... | 2009 | 19105955 |
| persistent, undetected trichomonas vaginalis infections? | 2009 | 19113985 | |
| trichomonosis and subsequent risk of prostate cancer in the prostate cancer prevention trial. | we previously observed a positive association between a history of trichomonosis, a sexually transmitted infection caused by the protozoan, trichomonas vaginalis, and prostate cancer risk in the health professionals follow-up study. to determine the reproducibility of this finding, we conducted a second, prospective investigation of trichomonosis and prostate cancer in the prostate cancer prevention trial. participants were men (>or=55 years of age) with no evidence of prostate cancer at enrollm ... | 2009 | 19117055 |
| patient teenagers? a comparison of the sexual behavior of virginity pledgers and matched nonpledgers. | the us government spends more than $200 million annually on abstinence-promotion programs, including virginity pledges. this study compares the sexual activity of adolescent virginity pledgers with matched nonpledgers by using more robust methods than past research. | 2009 | 19117832 |
| current issues and considerations regarding trichomoniasis and human immunodeficiency virus in african-americans. | trichomonas vaginalis has long been recognized as one of the most prevalent sexually transmitted infections. however, it is only in recent years that it has been appreciated that trichomonas may play a critical role in amplifying human immunodeficiency virus (hiv) transmission. given the evidence that t. vaginalis likely promotes hiv infection, the apparent high level of trichomonas infection in the african-american community is cause for concern. even if t. vaginalis increases the risk of hiv t ... | 2009 | 19136432 |
| transcriptional regulation of an iron-inducible gene by differential and alternate promoter entries of multiple myb proteins in the protozoan parasite trichomonas vaginalis. | iron-inducible transcription of a malic enzyme gene (also reputed to be ap65-1) in trichomonas vaginalis was previously shown to involve a myb1 repressor and a myb2 activator, each of which may preferentially select two closely spaced promoter sites, mre-1/mre-2r, which comprises overlapping promoter elements, and mre-2f. in the present study, an iron-inducible approximately 32-kda myb3 nuclear protein was demonstrated to bind only the mre-1 element. changes in the iron supply, which produced an ... | 2009 | 19151329 |
| identification and molecular characterization of numerous histomonas meleagridis proteins using a cdna library. | summaryhistomonas meleagridis is a protozoan parasite of various galliform birds causing a type of enterohepatitis termed histomonosis or 'blackhead disease'. due to the ban of chemotherapeutic substances and an increase in free-range poultry production, histomonosis is currently a re-emerging disease. so far limited molecular knowledge is available. in the present work, mrnas coding for antigenic proteins of h. meleagridis were identified. for this purpose, a cdna expression library was constru ... | 2009 | 19154645 |
| a comparison of cytological and parasitological methods in the diagnosis of trichomonas vaginalis. | trichomonas vaginalis (t. vaginalis), which causes urogenital system infections in humans, develops symptomatically and asymptomatically. t. vaginalis in females is diagnosed using direct microscopy, giemsa staining, and cultivation methods for examination of samples derived from the vaginal posterior fornix. serologic methods can also be employed. in cytological diagnosis, the ectocervical smear is examined using the papanicolaou (paps) stain. the aim of the present study was to compare the eff ... | 2008 | 19156601 |
| [killing effect of polymorphonuclear neutrophils on trichomonas vaginalis]. | to study the killing effect of polymorphonuclear neutrophils (pmns) on trichomonas vaginalis. | 2008 | 19157301 |
| [observation on mitosis of trichomonas vaginalis cultivated in vitro using modified giemsa staining]. | the observation showed that the percentage of trichomonas vaginalis trophozoites at the stages of interphase, binary fission and multiple fission was 66.5%, 24.1% and 9.4% respectively. cells in binary fission could be classified as premitotic phase, prophase, metaphase, anaphase and telophase. 3 to 8 microcosms were seen in one trophozoite under multiple fission and the percentage of trophozoites with 3 and 4 microcosms occupied 69% and 24.5% respectively. cells with abnormal morphs were also o ... | 2008 | 19157311 |
| [genetic variation and clustal analysis of trichomonas vaginalis cysteine proteases]. | to clone the genes coding for cysteine proteases (cps, tvcps) from trichomonas vaginalis and to analyze their genetic variations with the related sequences from ncbi database (genbank) and t. vaginalis genome project database from the institute for genomic research (tigr). | 2008 | 19160965 |
| cervicovaginal cytological abnormalities in patients with human immunodeficiency virus infection, in relation to disease stage, cd4 cell count and viral load. | the objective of the present study was to assess infections and cytologic abnormalities in cervicovaginal smears from 153 hiv-positive women and 169 hiv-negative followed up at the uftm school of medicine between may 1999 and may 2002. the medical records and cervicovaginal smears were reviewed and the hiv-positive group was classified according to cd4 cell count, hiv viral load, antiretroviral therapy and hiv subgroups (with or without disease; with or without therapy) and compared to hiv-negat ... | 2009 | 19170167 |
| beyond douching: use of feminine hygiene products and sti risk among young women. | use of feminine hygiene products (feminine wipes, sprays, douches, and yeast creams) by adolescent women is common, yet understudied. | 2009 | 19170863 |
| engineering escherichia coli for fermentative dihydrogen production: potential role of nadh-ferredoxin oxidoreductase from the hydrogenosome of anaerobic protozoa. | trichomonas vaginalis generates reduced ferredoxin within a unique subcellular organelle, hydrogenosome that is used as a reductant for h2 production. pyruvate ferredoxin oxidoreductase and nadh dehydrogenase (nadh-dh) are the two enzymes catalyzing the production of reduced ferredoxin. the genes encoding the two subunits of nadh-dh were cloned and expressed in escherichia coli. kinetic properties of the recombinant heterodimer were similar to that of the native enzyme from the hydrogenosome. th ... | 2009 | 19172236 |
| [trichomoniasis]. | trichomonas vaginalis is the most common curable sexually transmitted disease worldwide. in spite of serious health sequelae including facilitation of hiv transmission, pid, and adverse outcomes of pregnancy, it remains an underestimated condition, as a half of female infections and the majority of male infections are asymptomatic. t. vaginalis infection was not restricted in high-risk groups, so screening or empiric treatment of low-risk groups should be considered. to develop diagnostic tests ... | 2009 | 19177767 |
| can bacterial vaginosis help to find sexually transmitted diseases, especially chlamydial cervicitis? | this study was undertaken to establish reliable factors in order to identify chlamydial cervicitis among suspicious patients. between january and december 2007, 406 patients who were suspected to have cervicitis due to clinical symptoms, were tested with polymerase chain reaction (pcr) for chlamydia trachomatis (ct), vaginal ph and nugent score (ns) in our university hospital and related clinics. during the same period, 67 patients who were diagnosed as having other sexually transmitted diseases ... | 2009 | 19182056 |
| comparison of aptima trichomonas vaginalis transcription-mediated amplification to wet mount microscopy, culture, and polymerase chain reaction for diagnosis of trichomoniasis in men and women. | we evaluated the performance characteristics of aptima trichomonas vaginalis (atv) transcription-mediated amplification (tma) for diagnosis of t vaginalis (tv) infection from female vaginal swab, endocervical swab, and urine specimens and from male urethral swab and urine specimens. performance of atv tma was compared with wet mount microscopy, culture, and polymerase chain reaction (pcr). | 2009 | 19185101 |
| synthesis and antiprotozoal activity of novel 1-methylbenzimidazole derivatives. | in this paper are reported the synthesis and antiprotozoal activity in vitro of 24 1-methylbenzimidazole derivatives (13-36) substituted at position 2 with aminocarbonyl, n-methylaminocarbonyl, n,n-dimethylaminocarbonyl, ethoxycarbonyl, 1-hydroxyethyl and acetyl groups, some of them with chlorine atoms at the benzenoid ring. compounds 13-36 were more active than metronidazole, the choice drug against giardia intestinalis and most of them against trichomonas vaginalis. the most active group of co ... | 2009 | 19186059 |
| cysteine peptidase expression in trichomonas vaginalis isolates displaying high- and low-virulence phenotypes. | in the present study, we identified and characterized the cysteine peptidase (cp) profiles of trichomonas vaginalis isolates exhibiting high- and low-virulence phenotypes using a combination of two-dimensional sds-page (2de), tandem mass spectrometry (ms/ms), and data mining. seven of the eight cps identified belong to clan ca, family c1, cathepsin l-like cp, and one belongs to clan cd, family c13, asparaginyl endopeptidase-like cp. quantitative and qualitative differences in cp expression were ... | 2009 | 19186947 |
| genetic diversity of trichomonas vaginalis clinical isolates determined by ecori restriction fragment length polymorphism of heat-shock protein 70 genes. | restriction fragment length polymorphism (rflp) analysis using a multilocus heat-inducible cytoplasmic heat-shock protein 70 (hsp70) hybridization probe with ecori-digested genomic dna was used in molecular typing of 129 trichomonas vaginalis isolates. results indicate that trichomonas organisms exhibit considerable polymorphism in their hsp70 rflp patterns. analysis of seven american type culture collection reference strains and 122 clinical isolates, including 84 isolates from jackson, mississ ... | 2009 | 19190222 |
| [utility of molecular biology techniques in the diagnosis of sexually transmitted diseases and genital infections]. | historically, the diagnosis of sexually transmitted diseases (stds) has been difficult. the introduction of molecular biology techniques in microbiological diagnosis and their application to non-invasive samples has produced significant advances in the diagnosis of these diseases. overall, detection of neisseria gonorrhoeae by molecular biology techniques provides a presumptive diagnosis and requires confirmation by culture in areas with a low prevalence. for chlamydia trachomatis infections, th ... | 2008 | 19195446 |
| trichomonal sinusitis in an adolescent patient with multiple trauma. | trichomonal disease typically involves the genital and occasionally respiratory tracts. although exposure of the upper respiratory tract to infected genital secretions is not uncommon with contemporary sexual practices, trichomonal sinus disease has been rarely described. the present report describes the case of a healthy 17-year-old male admitted to an intensive care unit following multiple trauma, who developed purulent sinusitis on the 4th day of hospitalization. numerous trichomonads were no ... | 2009 | 19204647 |
| synthesis and in vitro trichomonicidal, giardicidal and amebicidal activity of n-acetamide(sulfonamide)-2-methyl-4-nitro-1h-imidazoles. | two new series of imidazole derivatives (acetamides: 1-8 and sulfonamides: 9-15) were synthesized using a short synthetic route. compound 1 as well as the intermediate 16g were characterized by x-ray crystallography. imidazole derivatives 1-15 were tested in vitro against three unicellular parasites (giardia intestinalis, trichomonas vaginalis and entamoeba histolytica) in comparison with benznidazole (bzn) and metronidazole. compound 1 [n-benzyl-2-(2-methyl-4-nitro-1h-imidazol-1-yl)acetamide] w ... | 2009 | 19208443 |
| infectivity of trichomonas vaginalis pseudocysts inoculated intra-vaginally in mice. | in addition to the trophozoite, pseudocyst is another morphological form which is recently identified among genitourinary trichomonads. although, this pseudocyst is competent to divide, its role in trichomonas life cycle has not yet been confirmed. in this study the ability of intra-vaginally inoculated t. vaginalis pseudocysts to induce trichomoniasis in infected mice was evaluated in comparison to the trophozoites. pseudocysts formation was induced by using thermal-freezing cycle method. the i ... | 2008 | 19209760 |
| comparison between trichmonas vaginalis symptomatic and asymptomatic isolates effects in intravaginally infected rats. | in this study, histopathological and immunohistochemical changes of the posterior vaginal fornix's and upper portion of the vagina were compared on rats infected with symptomatic and asymptomatic human isolates. eighteen symptomatic and asymptomatic female isolates were used (nine/ each). two groups of infected female rats were included in this study (3 rats /isolate). the results showed that there were no differences between symptomatic and asymptomatic isolates in histopathological changes; t. ... | 2008 | 19209767 |
| human papillomaviruses and genital co-infections in gynaecological outpatients. | high grade hpv infections and persistence are the strongest risk factors for cervical cancer. nevertheless other genital microorganisms may be involved in the progression of hpv associated lesions. | 2009 | 19216747 |
| validation of an immunologic diagnostic kit for infectious vaginitis by trichomonas vaginalis, candida spp., and gardnerella vaginalis. | fempure is a kit for the rapid diagnosis of vaginitis by trichomonas vaginalis, candida spp., and gardnerella vaginalis, based on aggregation of latex particles joined to specific antibodies. the validation of the method involved the parameters specificity, detection limit, robustness, clinical sensitivity, and clinical specificity. also, samples analyzed in parallel by the validated test and other recognized tests conducted by external laboratory were included. the method was specific for the 3 ... | 2009 | 19216938 |
| population-based assessment of mycoplasma genitalium in vietnam--low prevalence among married women of reproductive age in a rural area. | to analyse the prevalence of mycoplasma genitalium infection in a population-based study among married women from a demographic surveillance site in a rural geographical area of vietnam. | 2009 | 19220645 |
| association of public versus private health care utilization and prevalence of trichomonas vaginalis in maringá, paraná, brazil. | to determine the association of public versus private health care utilization and prevalence of trichomonas vaginalis in maringá, paraná, brazil. | 2009 | 19225796 |
| nmr structural analysis of dna recognition by a novel myb1 dna-binding domain in the protozoan parasite trichomonas vaginalis. | the transcription regulator, tvmyb1, is the first myb family protein identified in trichomonas vaginalis. using an electrophoretic mobility shift assay, we defined the amino-acid sequence from lys(35) to ser(141) (tvmyb1(35-141)) as the minimal dna-binding domain, encompassing two myb-like dna-binding motifs (designated as r2 and r3 motifs) and an extension of 10 residues at the c-terminus. nmr solution structures of tvmyb1(35-141) show that both the r2 and r3 motifs adopt helix-turn-helix confo ... | 2009 | 19246540 |
| multiplex pcr testing detection of higher-than-expected rates of cervical mycoplasma, ureaplasma, and trichomonas and viral agent infections in sexually active australian women. | knowing the prevalence of potential etiologic agents of nongonococcal and nonchlamydial cervicitis is important for improving the efficacy of empirical treatments for this commonly encountered condition. we describe four multiplex pcrs (mpcrs), designated vdl05, vdl06, vdl07, and vdl09, which facilitate the detection of a wide range of agents either known to be or putatively associated with cervicitis, including cytomegalovirus (cmv), enterovirus (ev), epstein-barr virus (ebv), varicella-zoster ... | 2009 | 19261782 |
| anal intercourse among young heterosexuals in three sexually transmitted disease clinics in the united states. | to examine factors associated with heterosexual anal intercourse (ai). | 2009 | 19265740 |
| prevalence of and risk factors for sexually-transmitted infections in hidden female sex workers. | we wanted to determine the age-specific prevalence of selected sexually transmitted infections while assessing the risk factors among hidden female sex workers (hfsw). | 2009 | 19269760 |
| horizontal gene transfer in eukaryotic parasites: a case study of entamoeba histolytica and trichomonas vaginalis. | over the past few years it has become apparent that horizontal gene transfer (hgt) has played an important role in the evolution of pathogenic prokaryotes. what is less clear is the exact role that hgt has played in shaping the metabolism of eukaryotic organisms. the main problems are the reliable inference of hgt on a genomic scale as well as the functional assignment of genes in these poorly studied organisms. we have screened the completed genomes of the protists entamoeba histolytica and tri ... | 2009 | 19271203 |
| prevalence of vulvovaginitis and bacterial vaginosis in patients with koilocytosis. | empirical discussion regarding an association between koilocytosis and vulvovaginitis often occurs. thus, the objective of this study was to assess the prevalence of microorganisms associated with bacterial vaginosis and vulvovaginitis in women with and without koilocytosis. | 2008 | 19274321 |
| validity of the symptomatic approach used by nurses in diagnosing vaginal infections. | to examine the validity and effectiveness of a symptomatic approach based on symptoms, administered by a nurse working in women's health area, in identifying vaginal infections in women with or without vaginal discharge. | 2009 | 19284438 |
| comparative analyses among the trichomonas vaginalis, trichomonas tenax, and tritrichomonas foetus 5s ribosomal rna genes. | the 5s ribosomal rna (5s rrna) is an essential component of ribosomes. throughout evolution, variation is found among 5s rrna genes regarding their chromosomal localization, copy number, and intergenic regions. in this report, we describe and compare the gene sequences, motifs, genomic copy number, and chromosomal localization of the trichomonas vaginalis, trichomonas tenax, and tritrichomonas foetus 5s rrna genes. t. vaginalis and t. foetus have a single type of 5s rrna-coding region, whereas t ... | 2009 | 19290527 |
| genetic identity and differential gene expression between trichomonas vaginalis and trichomonas tenax. | trichomonas vaginalis is a human urogenital pathogen responsible for trichomonosis, the number-one, non-viral sexually transmitted disease (std) worldwide, while t. tenax is a commensal of the human oral cavity, found particularly in patients with poor oral hygiene and advanced periodontal disease. the extent of genetic identity between t. vaginalis and its oral commensal counterpart is unknown. | 2009 | 19296850 |
| prospective study of correlates of vaginal lactobacillus colonisation among high-risk hiv-1 seronegative women. | vaginal colonisation with lactobacillus species is characteristic of normal vaginal ecology. the absence of vaginal lactobacilli, particularly hydrogen peroxide (h(2)o(2))-producing isolates, has been associated with symptomatic bacterial vaginosis (bv) and increased risk for hiv-1 acquisition. identification of factors associated with vaginal lactobacillus colonisation may suggest interventions to improve vaginal health. | 2009 | 19329442 |
| sexually transmitted infections and reproductive tract infections in female sex workers. | the present study was conducted in 300 female sex workers (fsws) from surat city in 2005-2006. vaginal swabs, endocervical swabs and serum samples were collected from each of these fsws. vaginal samples were screened for bacterial vaginosis (bv), candidiasis and trichomonas vaginalis . endocervical swabs were screened for gonococcal infection. serological tests for human immunodeficiency virus (hiv), hepatitis b surface antigen (hbsag) and syphilis were performed. from a total of 300 fsws, bv wa ... | 2009 | 19332911 |
| the microaerophilic flagellate, trichomonas vaginalis, contains unusual acyl lipids but no detectable cardiolipin. | previous lipid analysis of trichomonads has led to controversy as to whether these hydrogenosome-containing organisms contain cardiolipin (cl), which is a characteristic component of mitochondria. here we report a careful lipid analysis of the sexually transmitted protist trichomonas vaginalis. major lipids were phosphatidylethanolamine (42%) and phosphatidylcholine (20%) with lesser amounts of phosphatidylglycerol (pg) (12%) and non-polar components. two unusual lipids, acyl-pg (8%) and ceramid ... | 2009 | 19335774 |
| clinical significance of trichomonas vaginalis detected in papanicolaou smear: a survey in female social hygiene clinic. | to evaluate the clinical significance of trichomonas vaginalis detected in papanicolaou (pap) smears in our local population. | 2009 | 19342733 |
| association between group a beta-haemolytic streptococci and vulvovaginitis in adult women: a case-control study. | guidelines for the management of vaginal discharge mention candida albicans, trichomonas vaginalis, bacterial vaginosis, chlamydia trachomatis and neisseria gonorrhoeae as causes and do not recommend full microbiological culture. the role of non-group b beta-haemolytic streptococci in vaginal cultures is unclear, except for group a streptococci that are known to cause vulvovaginitis in children. in a case-control study, we investigated the association between non-group b beta-haemolytic streptoc ... | 2009 | 19343383 |
| simultaneous identification of 14 genital microorganisms in urine by use of a multiplex pcr-based reverse line blot assay. | the aim of this study was to develop and evaluate a sensitive method for the simultaneous identification of 14 urogenital potential pathogens. a multiplex pcr-based reverse line blot (mpcr/rlb) assay was developed to detect 14 urogenital pathogens or putative pathogens, namely trichomonas vaginalis, streptococcus pneumoniae, neisseria gonorrhoeae, chlamydia trachomatis, ureaplasma parvum, u. urealyticum, gardnerella vaginalis, haemophilus influenzae, herpes simplex virus type 1 (hsv1) and hsv2, ... | 2009 | 19357202 |
| glyceraldehyde-3-phosphate dehydrogenase is a surface-associated, fibronectin-binding protein of trichomonas vaginalis. | trichomonas vaginalis colonizes the urogenital tract of humans and causes trichomonosis, the most prevalent nonviral sexually transmitted disease. we have shown an association of t. vaginalis with basement membrane extracellular matrix components, a property which we hypothesize is important for colonization and persistence. in this study, we identify a fibronectin (fn)-binding protein of t. vaginalis. a monoclonal antibody (mab) from a library of hybridomas that inhibited the binding of t. vagi ... | 2009 | 19380472 |
| mycoplasma genitalium compared to chlamydia, gonorrhoea and trichomonas as an aetiological agent of urethritis in men attending std clinics. | to investigate prevalence of mycoplasma genitalium, chlamydia trachomatis, neisseria gonorrhoeae and trichomonas vaginalis in men, frequency of co-infections, and association of organisms with urethritis in men. | 2009 | 19383597 |
| high rates of std and sexual risk behaviors among garífunas in honduras. | honduras has the highest concentration of hiv and aids cases in central america, with an estimated adult hiv prevalence of 1.5%. prevalence is higher among certain ethnic groups such as the garífuna with a reported hiv prevalence of 8%. | 2009 | 19384098 |
| three-dimensional structure of the cytoskeleton in trichomonas vaginalis revealed new features. | the flagellated protozoan trichomonas vaginalis has been widely studied owing to its medical significance and unique structure. the complicated three-dimensional (3d) structure of the cellular components of t. vaginalis was reconstructed from serial sections to enable observation of the spatial features of the whole cell. electron tomography was used to examine the detailed structure of the cellular organelles. tomographic reconstruction showed the mastigont system and the parabasal filament of ... | 2009 | 19386993 |
| simple and inexpensive point-of-care tests improve diagnosis of vaginal infections in resource constrained settings. | bacterial vaginosis (bv) and trichomonas vaginalis infection (tv) have been associated with adverse birth outcomes and increased risk for hiv. we compare the performance of simple inexpensive point-of-care (poc) tests to laboratory diagnosis and syndromic management of bv and tv in poor settings. | 2009 | 19392745 |
| alfred francois donné (1801-78): a pioneer of microscopy, microbiology and haematology. | alfred françois donné is widely known in the scientific community as the discoverer of trichomonas vaginalis, since he was the first to illustrate the parasite that later was recognized to cause vaginal infections. however, his other, less-known findings are equally important: he was also the inventor of the photoelectric microscope, with the assistance of his student léon foucault, as well as the first to apply photography to microscopic preparations (daguerreotype). his research in microscopy ... | 2009 | 19401511 |
| characteristics of hiv-1 discordant couples enrolled in a trial of hsv-2 suppression to reduce hiv-1 transmission: the partners study. | the partners hsv-2/hiv-1 transmission study (partners study) is a phase iii, placebo-controlled trial of daily acyclovir for genital herpes (hsv-2) suppression among hiv-1/hsv-2 co-infected persons to reduce hiv-1 transmission to their hiv-1 susceptible partners, which requires recruitment of hiv-1 serodiscordant heterosexual couples. we describe the baseline characteristics of this cohort. | 2009 | 19404392 |
| use of a dual priming oligonucleotide system to detect multiple sexually transmitted pathogens in clinical specimens. | to evaluate a new dual priming oligonucleotide (dpo)-based multiplex polymerase chain reaction (pcr) assay for detection of six sexually transmitted pathogens, including chlamydia trachomatis, neisseria gonorrhoeae, mycoplasma genitalium, mycoplasma hominis, ureaplasma urealyticum and trichomonas vaginalis. | 2009 | 19413770 |
| trichomonas vaginalis: metronidazole and other nitroimidazole drugs are reduced by the flavin enzyme thioredoxin reductase and disrupt the cellular redox system. implications for nitroimidazole toxicity and resistance. | infections with the microaerophilic parasite trichomonas vaginalis are treated with the 5-nitroimidazole drug metronidazole, which is also in use against entamoeba histolytica, giardia intestinalis and microaerophilic/anaerobic bacteria. here we report that in t. vaginalis the flavin enzyme thioredoxin reductase displays nitroreductase activity with nitroimidazoles, including metronidazole, and with the nitrofuran drug furazolidone. reactive metabolites of metronidazole and other nitroimidazoles ... | 2009 | 19415801 |
| [steroidal saponins from the plant agave brittoniana with activity against the parasite trichomona vaginalis]. | the genus agave (agavaceae), includes more than 300 species; around 16 of them show an homogeneous distribution throughout cuba. agave brittoniana (ssp. brachypus), is an endemic subspecies that grows in the central region of the country and its leaves are traditionally used in the treatment of parasitic diseases. the parasite trichomonas vaginalis causes the disease known as trichomoniasis, that infects the genital tract. to test in vitro the plant against trichomona vaginalis, the dried and po ... | 2008 | 19419073 |
| comparison of clinical symptoms scored according to the national institutes of health chronic prostatitis symptoms index and assessment of antimicrobial treatment in patients with chronic prostatitis syndrome. | we examined a total of 194 patients over 18 years of age with chronic prostatitis syndrome and no evidence of structural or functional lower genitourinary tract abnormalities. the following data were obtained for each patient: clinical history--the severity of chronic prostatitis symptoms scored by a croatian translation of the nih cpsi questionnaire, clinical status including digitorectal examination, urethral swab specimens, and selective samples of urine and expressed prostatic secretion, acc ... | 2009 | 19423471 |
| disentangling contributions of reproductive tract infections to hiv acquisition in african women. | : to estimate the effects of reproductive tract infections (rtis) on hiv acquisition among zimbabwean and ugandan women. | 2009 | 19434010 |