Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| immune protection against lethal fungal-bacterial intra-abdominal infections. | polymicrobial intra-abdominal infections (iais) are clinically prevalent and cause significant morbidity and mortality, especially those involving fungi. our laboratory developed a mouse model of iai and demonstrated that intraperitoneal inoculation withcandida albicansor other virulent non-albicans candida(nac) species plusstaphylococcus aureusresulted in 70 to 80% mortality in 48 to 72 h due to robust local and systemic inflammation (sepsis). surprisingly, inoculation withcandida dubliniensiso ... | 2018 | 29339423 |
| positive autoregulation and repression of transactivation are key regulatory features of the candida glabrata pdr1 transcription factor. | resistance to azole drugs, the major clinical antifungal compounds, is most commonly due to gain-of-function (gof) substitution mutations in a gene called pdr1 in the fungal pathogen candida glabrata. pdr1 encodes a zinc cluster-containing transcription factor. gof forms of pdr1 drive high level expression of downstream target gene expression with accompanying azole resistance. pdr1 has two homologous genes in saccharomyces cerevisiae, called scpdr1 and scpdr3. this study provides evidence that ... | 2018 | 29363861 |
| whole genome sequencing of candida glabrata for detection of markers of antifungal drug resistance. | candida glabrata can rapidly acquire mutations that result in drug resistance, especially to azoles and echinocandins. identification of genetic mutations is essential, as resistance detected in vitro can often be correlated with clinical failure. we examined the feasibility of using whole genome sequencing (wgs) for genome-wide analysis of antifungal drug resistance in c. glabrata. the aim was torecognize enablers and barriers in the implementation wgs and measure its effectiveness. this paper ... | 2017 | 29364212 |
| novel naja atra cardiotoxin 1 (ctx-1) derived antimicrobial peptides with broad spectrum activity. | naja atra subsp. atra cardiotoxin 1 (ctx-1), produced by chinese cobra snakes, belonging to elapidae family, is included in the three-finger toxin family and exerts high cytotoxicity and antimicrobial activity too. using as template mainly the tip and the subsequent β-strand of the first "finger" of this toxin, different sequences of 20 amino acids linear peptides have been designed in order to avoid toxic effects but to maintain or even strengthen the partial antimicrobial activity already seen ... | 2018 | 29364903 |
| candida glabrata biofilms: how far have we come? | infections caused bycandidaspecies have been increasing in the last decades and can result in local or systemic infections, with high morbidity and mortality. aftercandida albicans,candida glabratais one of the most prevalent pathogenic fungi in humans. in addition to the high antifungal drugs resistance and inability to form hyphae or secret hydrolases,c. glabrataretain many virulence factors that contribute to its extreme aggressiveness and result in a low therapeutic response and serious recu ... | 2017 | 29371530 |
| azole resistance caused by increased drug efflux incandida glabrataisolated from the urinary tract of a dog with diabetes mellitus. | a yeast-like organism was isolated from a urine sample of a 6-year-old neutered male miniature poodle dog with urinary tract infection, diabetes ketoacidosis, and acute pancreatitis. we identified the yeast-like organism to becandida glabrataand found that this fungus was highly resistant to azole antifungal drugs. to understand the mechanism of azole resistance in this isolate, the sequences and expression levels of the genes involved in drug resistance were analyzed. the results of our analysi ... | 2017 | 29371812 |
| candida glabrata, friend and foe. | candida glabratais mostly good, but, at times, it is an opportunistic pathogen. previously known astorulopsis glabrata, it enjoyed a good reputation and was even present in starter cultures. its haploid genome and lack of mating made it an attractive challenge for yeast genetics studies. however, more recently it has become better known due to its character as an emerging cause of candidiasis, and for its resistance to multidrugs that are employed for candidiasis treatment. while now classified ... | 2015 | 29376912 |
| fluconazole non-susceptible breakthrough candidemia after prolonged low-dose prophylaxis: a prospective funginos study. | breakthrough candidemia (btc) on fluconazole was associated with non-susceptible candida spp. and increased mortality. this nationwide funginos study analyzed clinical and mycological btc characteristics. | 2018 | 29378240 |
| comparative study of the effects of fluconazole and voriconazole on candida glabrata, candida parapsilosis and candida rugosa biofilms. | infections by non-albicans candida species are a life-threatening condition, and formation of biofilms can lead to treatment failure in a clinical setting. this study was aimed to demonstrate the in vitro antibiofilm activity of fluconazole (flu) and voriconazole (vor) against c. glabrata, c. parapsilosis and c. rugosa with diverse antifungal susceptibilities to flu and vor. the antibiofilm activities of flu and vor in the form of suspension as well as pre-coatings were assessed by xtt [2,3-bis- ... | 2018 | 29380188 |
| activity and expression of candida glabrata vacuolar proteases in autophagy-like conditions. | candida glabrata is an emerging opportunistic pathogen that has intrinsic resistance to azoles antifungals. during infection or while living as a commensal it encounters nutritional stresses such as deficiency of carbon or nitrogen sources. herein we investigate the expression and activity of pra, ape1, ape3 and cpy vacuolar proteases during these stressful nutrimental conditions. our findings demonstrate a differential activity profile depending on the addition or lack of carbon, nitrogen or bo ... | 2018 | 29385574 |
| evaluation of synergistic anticandidal and apoptotic effects of ferulic acid and caspofungin against candida albicans. | this study aimed to investigate the synergy between anticandidal and apoptotic effects of ferulic acid and caspofungin against candida albicans and candida glabrata, with the help of a quantitative checkerboard microdilution assay using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (mtt) as a viability dye. apoptotic effects of caspofungin and ferulic acid concentrations (alone and combined) were analyzed for c. albicans and c. glabrata based on annexin v-propidium iodide binding ... | 2018 | 29389586 |
| molecular identification of candida species from urinary infections in honduras. | candiduria is a common infection among hospitalised patients. although the clinical relevance of yeasts in urine is not clearly defined, fungal urinary tract infections have increased significantly in the last decades, becoming a growing public health problem. candida albicans is the most commonly reported species in urinary infections, although other species of the genus are becoming particularly important, because some of them are linked with resistance to antifungal drugs. | 2018 | 29395833 |
| candidacidal activity of a novel killer toxin from wickerhamomyces anomalus against fluconazole-susceptible and -resistant strains. | the isolation and characterization from the sand flyphlebotomus perniciosusof awickerhamomyces anomalusyeast strain (wa1f1) displaying the killer phenotype was recently reported. in the present work, the killer toxin (kt) produced bywa1f1 was purified and characterized, and its antimicrobial activity in vitro was investigated against fluconazole- susceptible and -resistant clinical isolates and laboratory strains ofcandida albicansandc. glabratadisplaying known mutations.wa1f1-kt showed a differ ... | 2018 | 29401638 |
| the emerging threat of antifungal resistance in transplant infectious diseases. | the global emergence of antifungal resistance among candida spp. and aspergillus spp. will disproportionately affect transplantation recipients, who are prone to invasive fungal disease. | 2018 | 29404711 |
| identification of medically importantcandidaspecies by polymerase chain reaction-restriction fragment length polymorphism analysis of the rdna its1 and its2 regions. | we aimed to identify the distribution of species in candidal strains isolated from clinical samples and restriction fragment length polymorphism (rflp) method based on msp i and bln i restrictive enzyme cuts of polymerase chain reaction (pcr) products after the amplification of its1 and its2 regions of rdna genotypically. | 2018 | 29405146 |
| characterisation of an abc transporter of a resistant candida glabrata clinical isolate. | candida glabrata ranks second in epidemiological surveillance studies, and is considered one of the main human yeast pathogens. treatment of candida infections represents a contemporary public health problem due to the limited availability of an antifungal arsenal, toxicity effects and increasing cases of resistance. c. glabrata presents intrinsic fluconazole resistance and is a significant concern in clinical practice and in hospital environments. | 2018 | 29412345 |
| clinical characteristics of the first cases of invasive candidiasis in china due to pan-echinocandin-resistantcandida tropicalisandcandida glabrataisolates with delineation of their resistance mechanisms. | echinocandin antifungal agents have become the first-line therapy for invasive candidiasis (ic) in many countries. despite their increasing use, resistance to this class of drug is, overall, still uncommon. here, we report two patients from the people's republic of china with ic, one with infection caused by pan-echinocandin-resistantcandida tropicalisand the other by pan-echinocandin-resistantcandida glabrata. we also describe the mechanisms of drug resistance of these isolates. the echinocandi ... | 2018 | 29416360 |
| increased urine interleukin-17 and interleukin-22 levels in patients with candidal urinary tract infection. | candiduria is common in the hospitalized patients. this study aimed to quantify interleukin (il)-17 and il-22 levels in urine of candiduric patients. | 2018 | 29421775 |
| relative abundance ofcandida albicansandcandida glabratainin vitroco-culture biofilms impacts biofilm structure and formation. | candidais a member of the normal human microbiota and often resides on mucosal surfaces such as the oral cavity or the gastrointestinal tract. in addition to their commensality,candidaspecies can opportunistically become pathogenic if the host microbiota is disrupted or if the host immune system becomes compromised. an important factor forcandidapathogenesis is its ability to form biofilm communities. the two most medically important species -candida albicansandcandida glabrata- are often co-iso ... | 2018 | 29427422 |
| yeasts from skin colonization are able to cross the acellular dermal matrix. | in recent decades, the prognosis for burn patients has improved considerably with the development of specialized care. the acellular dermal matrix (adm) is a totally artificial acellular device that functions to control water loss, prevent penetration by bacteria and allow migration of endothelial cells and fibroblasts from patient tissues. however, little is known about its effectiveness against yeasts. the present study evaluated the capacity of colonization and migration of some human commens ... | 2018 | 29428422 |
| [a clinical analysis of children with invasive pulmonary fungal infections after biliary atresia surgery]. | to investigate the clinical features of invasive pulmonary fungal infections (ipfis) after biliary atresia (ba) surgery and related risk factors. | 2018 | 29429460 |
| oral yeast colonization and fungal infections in peritoneal dialysis patients: a pilot study. | peritonitis and exit-site infections are important complications in peritoneal dialysis (pd) patients that are occasionally caused by opportunistic fungi inhabiting distant body sites. in this study, the oral yeast colonization of pd patients and the antifungal susceptibility profile of the isolated yeasts were accessed and correlated with fungal infection episodes in the following 4 years. saliva yeast colonization was accessed in 21 pd patients and 27 healthy controls by growth in chromagar-ca ... | 2017 | 29430252 |
| detecting infections rapidly and easily for candidemia trial, part 2 (direct2): a prospective, multicenter study of the t2candida panel. | blood cultures are approximately 50% sensitive for diagnosing invasive candidiasis. the t2candida nanodiagnostic panel uses t2 magnetic resonance and a dedicated instrument to detect candida directly within whole blood samples. | 2018 | 29438475 |
| in vitroandin vivoefficacy of a novel and long acting fungicidal azole, pc1244 onaspergillus fumigatusinfection. | the antifungal effects of the novel triazole, pc1244, designed for topical or inhaled administration, againsta. fumigatushave been tested in a range ofin vitroandin vivostudies. pc1244 demonstrated potent antifungal activities against clinicala. fumigatusisolates (n=96) with a mic range of 0.016--0.25 μg/ml, whereas the mic range for voriconazole was 0.25--0.5 μg/ml. pc1244 was a strong tight-binding inhibitor of recombinanta. fumigatuscyp51a and cyp51b (sterol 14α-demethylase) enzymes and stron ... | 2018 | 29439966 |
| a quest to find good primers for gene expression analysis of candida albicans from clinical samples. | biofilm production contributes to several human diseases, including oral candidiasis. among the candida species, candida albicans is the most prevalent. the expression of virulence genes is implicated in the pathogenic potential of candida biofilms. however, the evaluation of microbial gene expression from in vivo biofilm samples is not trivial, specifically, assessment via quantitative pcr (qpcr) can be a challenge because of several species present in clinical samples. hence, the necessity of ... | 2018 | 29454005 |
| multiple brain abscesses caused by infection withcandida glabrata: a case report. | the present case report described the initial diagnosis of a 25-year old female with a brain abscess consisting of two lesions 0.2 and 2.9 cm3in volume. the patient was initially treated with antibiotics; however, 2 months following initial treatment, the patient's condition deteriorated and she became vegetative. following transfer to the china-japan union hospital of jilin university (jilin, china) the two lesions had grown in volume to 9.0 and 13.0 cm3, respectively. the results of magnetic r ... | 2018 | 29456643 |
| fibrin web in a patient with candida glabrata endophthalmitis. | 2018 | 29458827 | |
| comparative virulence of candida auris with candida haemulonii, candida glabrata and candida albicans in a murine model. | the incidence of invasive fungal infections (ifis) caused by uncommon candida species with diverse virulence and susceptibility profiles has increased in recent years. due to scarce clinical and experimental data on the pathogenicity of candida auris, the aim of this study was to evaluate and compare the virulence of two rare clinically relevant species, c. auris and candida haemulonii with candida glabrata and candida albicans in an immunocompetent murine model of disseminated infection. immuno ... | 2018 | 29460345 |
| remodeling of the candida glabrata cell wall in the gastrointestinal tract affects the gut microbiota and the immune response. | the gastrointestinal (gi) microbiota acts a natural barrier to the proliferation of opportunistic pathogens. candida glabrata is an opportunistic yeast pathogen that has adapted to colonize all segments of the human gi tract. we observed an increase in escherichia coli, enterococcus faecalis, and bacteroides vulgatus populations, and a decrease in lactobacillus johnsonii, bacteroides thetaiotaomicron, and bifidobacterium animalis in mice with dss-induced colitis. this reduction was more pronounc ... | 2018 | 29463799 |
| cgpdr1 gain-of-function mutations lead to azole-resistance and increased adhesion in clinical candida glabrata strains. | recently, candida glabrata has emerged as a health-threatening pathogen and the rising resistance to antifungal agent in c. glabrata often leads to clinical treatment failure. | 2018 | 29464833 |
| clinical features, antimicrobial susceptibilities, and treatment outcomes of patients with culture positive endophthalmitis after penetrating keratoplasty. | to report the clinical features organisms and treatment outcomes in patients with endophthalmitis after penetrating keratoplasty (pk). | 2018 | 29468222 |
| reduced antifungal susceptibility of vulvovaginal candida species at normal vaginal ph levels: clinical implications. | the aims of the study were to assess for differences in antifungal in vitro potency at ph 7 and ph 4 and to discuss any potential clinical implications of that difference. | 2018 | 29474241 |
| antifungal activity of the root extracts of pulsatilla patens against candida glabrata. | plants from the genus of pulsatilla produce a variety of secondary metabolites with biological activity. these species play a special role in herbal medicine and are used in traditional folk medicine to treat many diseases and ailments. due to their numerous medicinal properties, they are now also widely used as homeopathic preparations. in the present study, the antifungal activity of crude extracts of the root of pulsatilla patens (l.) mill. against the yeast candida glabrata with an ic₅₀ of 9 ... | 2017 | 29474774 |
| an integrated process for xylooligosaccharide and bioethanol production from corncob. | an integrated process for xylooligosaccharides (xos) and bioethanol production from corncob was investigated. xos were produced by a consecutive process of koh treatment and hydrolysis by an in-house thermostable endo-xylanase from streptomyces thermovulgaris. xo yields of 0.15 g/gkoh-treated corncob(22.13 g/l) and 0.52 g/graw corncobof cellulose-rich corncob (crc) were obtained. after 96 h of enzymatic hydrolysis, crc hydrolysate contained 62.16, 51.21, 10.03 and 0.92 g/l of total sugar, glucos ... | 2018 | 29475148 |