Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
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| physicochemical and genetic analysis of an endemic rice variety, njavara (oryza sativa l.), in comparison to two popular south indian cultivars, jyothi (ptb 39) and ir 64. | njavara is a medicinal rice strain, endemic to kerala, south india, bestowed with medicinal qualities. genetic variations and some of the physicochemical properties were studied using standard molecular protocols and compared with those of nonmedicinal rice varieties: jyothi and ir 64. njavara showed 11 unique positive and 36 unique negative markers to differentiate it from jyothi and ir 64. genetic similarity coefficient studies showed two well-defined clusters separating njavara from jyothi an ... | 2009 | 19924858 |
| the gibberellic acid stimulated-like gene family in maize and its role in lateral root development. | in an approach to study lateral root development in monocots, genome-wide searches for homologs of the gibberellic acid stimulated transcript-like (gast-like) gene family in rice (oryza sativa) and maize (zea mays) were carried out. six novel gast-like genes in rice and 10 members of the gene family in maize, which were designated zmgsl (for z. mays gibberellic acid stimulated-like), were identified. the zmgsl family encodes small proteins of 75 to 128 amino acids, which are characterized by a c ... | 2010 | 19926801 |
| genetic resources for maize cell wall biology. | grass species represent a major source of food, feed, and fiber crops and potential feedstocks for biofuel production. most of the biomass is contributed by cell walls that are distinct in composition from all other flowering plants. identifying cell wall-related genes and their functions underpins a fundamental understanding of growth and development in these species. toward this goal, we are building a knowledge base of the maize (zea mays) genes involved in cell wall biology, their expression ... | 2009 | 19926802 |
| evidence for the mechanisms of zinc uptake by rice using isotope fractionation. | in an earlier study, we found that rice (oryza sativa) grown in nutrient solution well-supplied with zn preferentially took up light (64)zn over (66)zn, probably as a result of kinetic fractionation in membrane transport processes. here, we measure isotope fractionation by rice in a submerged zn-deficient soil with and without zn fertilizer. we grew the same genotype as in the nutrient solution study plus low-zn tolerant and intolerant lines from a recombinant inbred population. in contrast to t ... | 2010 | 19929899 |
| the role of lateral roots in bypass flow in rice (oryza sativa l.). | although an apoplastic pathway (the so-called bypass flow) is implicated in the uptake of na(+) by rice growing in saline conditions, the point of entry of this flow into roots remains to be elucidated. we investigated the role of lateral roots in bypass flow using the tracer trisodium-8-hydroxy-1,3,6-pyrenetrisulphonic acid (pts) and the rice cv. ir36. pts was identified in the vascular tissue of lateral roots using both epifluorescence microscopy and confocal laser scanning microscopy. cryo-sc ... | 2010 | 19930130 |
| studies on sodium bypass flow in lateral rootless mutants lrt1 and lrt2, and crown rootless mutant crl1 of rice (oryza sativa l.). | an apoplastic pathway, the so-called bypass flow, is important for na+ uptake in rice (oryza sativa l.) under saline conditions; however, the precise site of entry is not yet known. we report the results of our test of the hypothesis that bypass flow of na+ in rice occurs at the site where lateral roots emerge from the main roots. we investigated na+ uptake and bypass flow in lateral rootless mutants (lrt1, lrt2), a crown rootless mutant (crl1), their wild types (oochikara, nipponbare and taichu ... | 2010 | 19930131 |
| in plants, expression breadth and expression level distinctly and non-linearly correlate with gene structure. | compactness of highly/broadly expressed genes in human has been explained as selection for efficiency, regional mutation biases or genomic design. however, highly expressed genes in flowering plants were shown to be less compact than lowly expressed ones. on the other hand, opposite facts have also been documented that pollen-expressed arabidopsis genes tend to contain shorter introns and highly expressed moss genes are compact. this issue is important because it provides a chance to compare the ... | 2009 | 19930585 |
| genetic analysis and molecular mapping of a novel gene for zebra mutation in rice (oryza sativa l.). | a novel zebra mutant, zebra-15, derived from the restorer line jinhui10 (oryza sativa l. ssp. indica) treated by ems, displayed a distinctive zebra leaf from seedling stage to jointing stage. its chlorophyll content decreased (55.4%) and the ratio of chla/chlb increased (90.2%) significantly in the yellow part of the zebra-15, compared with the wild type. net photosynthetic rate and fluorescence kinetic parameters showed that the decrease of chlorophyll content significantly influenced the photo ... | 2009 | 19932464 |
| [mapping of qtls of cd2+ content in brown rice under cd2+ stress in rice]. | a population of f8 recombinant inbred lines (rils), derived from a cross between ir26 (oryza sativa l. spp. indica) and jiucaiqing (japonica), were used to identify the quantitative trait loci (qtls) for cd2+ content in brown rice under 5 mg/kg cd2+ stress. two qtls, qccbr-11a and qccbr-11b, associated with the cd2+ content in brown rice, were detected on chromosome 11. qccbr-11a was located at the position between markers rm6288 and rm6544, accounting for 11.17% of the phenotypic variance with ... | 2009 | 19933095 |
| knock-down of osdcl2 in rice negatively affects maintenance of the endogenous dsrna virus, oryza sativa endornavirus. | an endogenous double-stranded rna (dsrna), which has recently been recognized as the dsrna virus oryza sativa endornavirus (osev), is found in many strains of cultivated rice (oryza sativa). small rnas derived from osev dsrna were detected, indicating that the rna silencing machinery recognizes osev dsrna. the existence of osev in knock-down (kd) lines of five genes of rna-dependent rna polymerase (osrdr1-osrdr5) or two genes of dicer-like protein (osdcl2 or osdcl3a) was examined to characterize ... | 2010 | 19933266 |
| the syncytium-specific expression of the orysa;krp3 cdk inhibitor: implication of its involvement in the cell cycle control in the rice (oryza sativa l.) syncytial endosperm. | during rice (oryza sativa l.) seed development, the primary endosperm nucleus undergoes a series of divisions without cytokinesis, producing a multinucleate cell, known as a syncytium. after several rounds of rapid nuclear proliferation, the syncytium ceases to undergo mitosis; thereafter, the syncytium is partitioned into individual cells by a specific type of cytokinesis called cellularization. the transition between syncytium and cellularization is important in determining the final seed size ... | 2010 | 19933315 |
| erect panicle2 encodes a novel protein that regulates panicle erectness in indica rice. | rice (oryza sativa l.) inflorescence (panicle) architecture is an important agronomic trait for rice breeding. a number of high-yielding japonica rice strains, characterized by an erect panicle (ep) of their architecture, have been released as commercial varieties in china. but no ep-type indica varieties are released so far. here, we identified two allelic erect-panicle mutants in indica rice, erect panicle2-1 (ep2-1) and erect panicle2-2 (ep2-2), exhibiting the characteristic erect panicle phe ... | 2010 | 19933874 |
| gene expression profiles in rice roots under low phosphorus stress. | phosphorus (p), an important plant macronutrient, is a component of key molecules such as nucleic acids, phospholipids and atp. p is often the limiting nutrient for crop yield potential because of the low concentration of soluble p that can be absorbed directly by plant. plants have evolved a series of molecular and morphological adaptations to cope with p limitation. however, the molecular bases of these responses to p deficiency have not been thoroughly elucidated. in this report, the gene exp ... | 2010 | 19936943 |
| evidence for a role of hexokinases as conserved glucose sensors in both monocot and dicot plant species. | the role of the hexokinases (hxks) as glucose (glc) sensors has been mainly demonstrated for arabidopsis (arabidopsis thaliana) hxk1 (athxk1) but has yet to be shown in other plant species. in our recent publication, we reported that two rice (oryza sativa) hxks, oshxk5 and oshxk6, also function as glc sensors. these two enzymes harbor both mitochondrial targeting peptides (mtps) and nuclear localization signals (nlss), and we confirmed their dual-targeting ability to nuclei and mitochondria usi ... | 2009 | 19938377 |
| origin and diversification of basic-helix-loop-helix proteins in plants. | basic helix-loop-helix (bhlh) proteins are a class of transcription factors found throughout eukaryotic organisms. classification of the complete sets of bhlh proteins in the sequenced genomes of arabidopsis thaliana and oryza sativa (rice) has defined the diversity of these proteins among flowering plants. however, the evolutionary relationships of different plant bhlh groups and the diversity of bhlh proteins in more ancestral groups of plants are currently unknown. in this study, we use whole ... | 2010 | 19942615 |
| pericarp color and haplotype diversity in weedy rice (o. sativa f. spontanea) from thailand. | weedy rice (oryza sativa f. spontanea) is an important resource for breeding and evolutionary studies. the present study was carried out to identify the genotype of weedy rice strains responsible for variation of pericarp color by sequencing exon 6 of the rc gene. haplotype analysis revealed that rice strains with light red- and red-pericarp lacked the 14 bp deletion in the rc gene, while rice strains with white-, brown and light green-pericarp contained the 14 bp deletion. a single nucleotide p ... | 2009 | 19943464 |
| assessing the arsenic-contaminated rice (oryza sativa) associated children skin lesions. | the purpose of this study was to assess the potential risk of children skin lesions from arsenic-contaminated rice (oryza sativa) consumption in west bengal (india). published age- and gender-specific skin lesions data in west bengal were reanalyzed and incorporated into a weibull dose-response model to predict children skin lesion prevalence. monomethylarsonous acid (mma(iii)) levels in urine was used as a biomarker that could be predicted from a human physiologically based pharmacokinetic (pbp ... | 2010 | 19945215 |
| fine mapping of a gene causing hybrid pollen sterility between yunnan weedy rice and cultivated rice (oryza sativa l.) and phylogenetic analysis of yunnan weedy rice. | weedy rice represents an important resource for rice improvement. the f(1) hybrid between the japonica wide compatibility rice cultivar 02428 and a weedy rice accession from yunnan province (sw china) suffered from pollen sterility. pollen abortion in the hybrid occurred at the early bicellular pollen stage, as a result of mitotic failure in the microspore, although the tapetum developed normally. genetic mapping in a bc(1)f(1) population (02428//yunnan weedy rice (ywr)/02428) showed that a majo ... | 2010 | 19946705 |
| mapping and validation of quantitative trait loci for spikelets per panicle and 1,000-grain weight in rice (oryza sativa l.). | this study identified four and five quantitative trait loci (qtls) for 1,000-grain weight (tgw) and spikelets per panicle (spp), respectively, using rice recombinant inbred lines. qtls for the two traits (spp3a and tgw3a, tgw3b and spp3b) were simultaneously identified in the two intervals between rm3400 and rm3646 and rm3436 and rm5995 on chromosome 3. to validate qtls in the interval between rm3436 and rm5995, a bc(3)f(2) population was obtained, in which tgw3b and spp3b were simultaneously ma ... | 2010 | 19949766 |
| dissection of a qtl reveals an adaptive, interacting gene complex associated with transgressive variation for flowering time in rice. | a days to heading qtl (dth1.1) located on the short arm of rice chromosome 1 was sub-divided into eight sub-introgression lines (sils) to analyze the genetic basis of transgressive variation for flowering time. each sil contained one or more introgression(s) from o. rufipogon in the genetic background of the elite oryza sativa cultivar, jefferson. each introgression was defined at high resolution using molecular markers and those in the dth1.1 region were associated with the presence of one or m ... | 2010 | 19949767 |
| evaluation of elastic modulus and hardness of crop stalks cell walls by nano-indentation. | agricultural biomaterials such as crop stalks are natural sources of cellulosic fiber and have great potential as reinforced materials in bio-composites. in order to evaluate their potential as materials for reinforcement, the nano-mechanical properties of crop-stalk cell walls, i.e. those of cotton (gossypium herbaceu) stalk, soybean (glycine max) stalk, cassava (manihot esculent) stalk, rice (oryza sativa l.) straw, and wheat (triticum aestivum l.) straw, were investigated by means of nano-ind ... | 2010 | 19954968 |
| enhanced expression of alternative oxidase genes is involved in the tolerance of rice (oryza sativa l.) seedlings to drought stress. | drought stress significantly enhanced the capacity of the alternative respiratory pathway and induced aox1a and aox1b transcripts in rice seedling leaves. the drought-stressed seedlings pretreated with the inhibitor of the alternative respiratory pathway, 1 mm salicylhydroxamic acid, had a lower level of relative water content than the seedlings either subjected to drought or salicylhydroxamic acid treatment alone. this observation suggests that the alternative respiratory pathway could play a r ... | 2015 | 19957440 |
| single nucleotide polymorphisms in a gene for translation initiation factor (eif4g) of rice (oryza sativa) associated with resistance to rice tungro spherical virus. | rice tungro disease (rtd) is a serious constraint to rice production in south and southeast asia. rtd is caused by rice tungro spherical virus (rtsv) and rice tungro bacilliform virus. rice cv. utri merah is resistant to rtsv. to identify the gene or genes involved in rtsv resistance, the association of genotypic and phenotypic variations for rtsv resistance was examined in backcross populations derived from utri merah and rice germplasm with known rtsv resistance. genetic analysis revealed that ... | 2010 | 19958136 |
| grain-filling problem in 'super' rice. | modern rice (oryza sativa l.) cultivars, especially the newly bred 'super' rice, have numerous spikelets on a panicle with a large yield capacity. however, these cultivars often fail to achieve their high yield potential due to poor grain-filling of later-flowering inferior spikelets (in contrast to the earlier-flowering superior spikelets). conventional thinking to explain the poor grain-filling is the consequence of carbon limitation. recent studies, however, have shown that carbohydrate suppl ... | 2010 | 19959608 |
| deciphering the proteomic profile of rice (oryza sativa) bran: a pilot study. | the exact knowledge of the qualitative and quantitative protein components of rice bran is an essential aspect to be considered for a better understanding of the functional properties of this resource. aim of the present investigation was to extract the largest number of rice bran proteins and to obtain their qualitative characterization. for this purpose, three different extraction protocols have been applied either on full-fat or on defatted rice bran. likewise, to identify the highest number ... | 2009 | 19960476 |
| root-uptake of (14)c derived from acetic acid and (14)c transfer to rice edible parts. | three types of culture experiments using paddy rice (oryza sativa l.) were performed to examine root-uptake of (14)c in the form of acetic acid: double pot experiment (hydroponics), wet culture experiment (submerged sand medium), and chamber experiment (hydroponics and submerged sand medium). the (14)c radioactivity in the plant, mediums, and atmospheric carbon dioxide ((14)co(2)) in the chamber were determined, and the distribution of (14)c in the plant was visualized using autoradiography. in ... | 2010 | 19962904 |
| reactive oxygen species are involved in plant defense against a gall midge. | reactive oxygen species (ros) play a major role in plant defense against pathogens, but evidence for their role in defense against insects is still preliminary and inconsistent. in this study, we examined the potential role of ros in defense of wheat (triticum aestivum) and rice (oryza sativa) against hessian fly (mayetiola destructor) larvae. rapid and prolonged accumulation of hydrogen peroxide (h(2)o(2)) was detected in wheat plants at the attack site during incompatible interactions. increas ... | 2010 | 19965963 |
| effects of colonization of a bacterial endophyte, azospirillum sp. b510, on disease resistance in rice. | agriculturally important grasses contain numerous diazotrophic bacteria, the interactions of which are speculated to have some other benefits to the host plants. in this study, we analyzed the effects of a bacterial endophyte, azospirillum sp. b510, on disease resistance in host rice plants. rice plants (oryza sativa cv. nipponbare) were inoculated with b510 exhibited enhanced resistance against diseases caused by the virulent rice blast fungus magnaporthe oryzae and by the virulent bacterial pa ... | 2009 | 19966496 |
| a rare sugar, d-allose, confers resistance to rice bacterial blight with upregulation of defense-related genes in oryza sativa. | we investigated responses of rice plant to three rare sugars, d-altrose, d-sorbose, and d-allose, due to establishment of mass production methods for these rare sugars. root growth and shoot growth were significantly inhibited by d-allose but not by the other rare sugars. a large-scale gene expression analysis using a rice microarray revealed that d-allose treatment causes a high upregulation of many defense-related, pathogenesis-related (pr) protein genes in rice. the pr protein genes were not ... | 2010 | 19968553 |
| copper delivery by the copper chaperone for chloroplast and cytosolic copper/zinc-superoxide dismutases: regulation and unexpected phenotypes in an arabidopsis mutant. | copper (cu) is an important mineral nutrient found in chloroplasts as a cofactor associated with plastocyanin and cu/zn superoxide dismutase (cu/znsod). superoxide dismutases are metallo-enzymes found in most oxygenic organisms with proposed roles in reducing oxidative stress. several recent studies in arabidopsis have shown that micrornas and a squamosa promoter binding protein-like7 (spl7) transcription factor function to down-regulate the expression of many cu-proteins, including cu/znsod in ... | 2009 | 19969519 |
| structural differences among alkali-soluble arabinoxylans from maize (zea mays), rice (oryza sativa), and wheat (triticum aestivum) brans influence human fecal fermentation profiles. | human fecal fermentation profiles of maize, rice, and wheat bran and their dietary fiber fractions released by alkaline-hydrogen peroxide treatment (principally arabinoxylan) were obtained with the aim of identifying and characterizing fractions associated with high production of short chain fatty acids and a linear fermentation profile for possible application as a slowly fermentable dietary fiber. the alkali-soluble fraction from maize bran resulted in the highest short chain fatty acid produc ... | 2010 | 20000566 |
| functional conservation and diversification of class e floral homeotic genes in rice (oryza sativa). | mutant analyses in different eudicotyledonous flowering plants demonstrated that sepallata-like mads-box genes are required for the specification of sepals, petals, stamens and carpels, and for floral determinacy, thus defining class e floral organ identity genes. sep-like genes encode mads-domain transcription factors and constitute an angiosperm-specific gene clade whose members show remarkably different degrees of redundancy and sub-functionalization within eudicots. to better understand the ... | 2010 | 20003164 |
| a recent duplication revisited: phylogenetic analysis reveals an ancestral duplication highly-conserved throughout the oryza genus and beyond. | the role of gene duplication in the structural and functional evolution of genomes has been well documented. analysis of complete rice (oryza sativa) genome sequences suggested an ancient whole genome duplication, common to all the grasses, some 50-70 million years ago and a more conserved segmental duplication between the distal regions of the short arms of chromosomes 11 and 12, whose evolutionary history is controversial. | 2009 | 20003305 |
| changes in chloroplast ultrastructure, fatty acid components of thylakoid membrane and chlorophyll a fluorescence transient in flag leaves of a super-high-yield hybrid rice and its parents during the reproductive stage. | in plants, it is well established that chloroplast is one of the early targgeted organelles to breakdown during leaves senescing. here we applied a newly developed super-high-yield hybrid rice (oryza sativa) liangyoupeijiu (lypj) and its parents lines to investigate changes in ultrastructure of chloroplasts, fatty acid composition of thylakoid membrane lipids and chlorophyll (chl) a fluorescence transient in natural senescing leaves. we found that at full expansion of flag leaves in three lines, ... | 2010 | 20004497 |
| a raf-like mapkkk gene dsm1 mediates drought resistance through reactive oxygen species scavenging in rice. | mitogen-activated protein kinase (mapk) cascades have been identified in various signaling pathways involved in plant development and stress responses. we identified a drought-hypersensitive mutant (drought-hypersensitive mutant1 [dsm1]) of a putative mapk kinase kinase (mapkkk) gene in rice (oryza sativa). two allelic dsm1 mutants were more sensitive than wild-type plants to drought stress at both seedling and panicle development stages. the dsm1 mutants lost water more rapidly than wild-type p ... | 2010 | 20007444 |
| the role of casein kinase ii in flowering time regulation has diversified during evolution. | casein kinase ii (ck2) is a protein kinase with an evolutionarily conserved function as a circadian clock component in several organisms, including the long-day plant arabidopsis (arabidopsis thaliana). the circadian clock component circadian clock associated1 (cca1) is a ck2 target in arabidopsis, where it influences photoperiodic flowering. in rice (oryza sativa), a short-day plant, heading date6 (hd6) encodes a ck2alpha subunit that delays flowering time under long-day conditions. here, we de ... | 2010 | 20007447 |
| dynamic landscapes of four histone modifications during deetiolation in arabidopsis. | although landscapes of several histone marks are now available for arabidopsis thaliana and oryza sativa, such profiles remain static and do not provide information about dynamic changes of plant epigenomes in response to developmental or environmental cues. here, we analyzed the effects of light on four histone modifications (acetylation and trimethylation of lysines 9 and 27 on histone h3: h3k9ac, h3k9me3, h3k27ac, and h3k27me3, respectively). our genome-wide profiling of h3k9ac and h3k27ac re ... | 2009 | 20008096 |
| antagonistic hlh/bhlh transcription factors mediate brassinosteroid regulation of cell elongation and plant development in rice and arabidopsis. | in rice (oryza sativa), brassinosteroids (brs) induce cell elongation at the adaxial side of the lamina joint to promote leaf bending. we identified a rice mutant (ili1-d) showing an increased lamina inclination phenotype similar to that caused by br treatment. the ili1-d mutant overexpresses an hlh protein homologous to arabidopsis thaliana paclobutrazol resistance1 (pre1) and the human inhibitor of dna binding proteins. overexpression and rna interference suppression of ili1 increase and reduc ... | 2009 | 20009022 |
| characterization of allergenic epitopes of ory s1 protein from oryza sativa and its homologs. | vaccination is the most effective technique suggested now days for allergy treatment. recombinant-based approaches are mostly focused on genetic modification of allergens to produce molecules with reduced allergenic activity and conserved antigenicity. the molecules developed for vaccination in allergy possess significantly reduced allergenicity in terms of ige binding, and therefore will not lead to anaphylactic reactions upon injection. this approach is probably feasible with every peptide all ... | 2009 | 20011147 |
| fine mapping of a gene for non-pollen type thermosensitive genic male sterility in rice (oryza sativa l.). | the thermo-sensitive genic male sterility (tgms) lines play a crucial role in two-line hybrid rice production. for a practical tgms line, the stability of male sterility is one of the most important technical indicators. in this study, xians, a spontaneous mutant with stable male sterility from an indica rice cultivar xianhuangzhan, was classified as a non-pollen type tgms line. the critical non-pollen sterility point temperature of xians was determined as 27 degrees c. genetic analysis demonstr ... | 2010 | 20012261 |
| identification and analysis of qtls controlling cold tolerance at the reproductive stage and validation of effective qtls in cold-tolerant genotypes of rice (oryza sativa l.). | low temperature or cold stress is one of the major constraints of rice production and productivity in temperate rice-growing countries and high-altitude areas in the tropics. even though low temperature affects the rice plant in all stages of growth, the percent seed set is damaged severely by cold and this reduces the yield potential of cultivars significantly. in this study, a new source of cold-tolerant line, ir66160-121-4-4-2, was used as a donor parent with a cold-sensitive cultivar, geumob ... | 2010 | 20012263 |
| selenium accumulation by forage and grain crops and volatilization from seleniferous soils amended with different organic materials. | greenhouse and field experiments were conducted to study the direct and residual effect of applying different organic materials on se accumulation by crops and volatilization from seleniferous soils of northwestern india. among organic manures, press mud and poultry manures proved 4-5 times more effective in reducing se accumulation by different crops than farmyard manure. efficiency of organic manures increased with increase in application rates. application of both press mud and poultry manure ... | 2010 | 20015536 |
| fine mapping and candidate gene analysis of the floury endosperm gene, flo(a), in rice. | in addition to its role as an energy source for plants, animals and humans, starch is also an environmentally friendly alternative to fossil fuels. in rice, the eating and cooking quality of the grain is determined by its starch properties. the floury endosperm of rice has been explored as an agronomical trait in breeding and genetics studies. in the present study, we characterized a floury endosperm mutant, flo(a), derived from treatment of oryza sativa ssp. japonica cultivar hwacheong with mnu ... | 2010 | 20016946 |
| characterization of interspecific hybrids and backcross progenies from a cross between oryza minuta and oryza sativa. | oryza minuta, a tetraploid wild relative of cultivated rice, is an important source for the genetic improvement. interspecific hybrids were obtained from the cross of o. sativa l. (ir24) and o. minuta (acc. no. 101133) with 5.58% crossability, which ranged from 0.11% to 1.62% in the backcross generations. the chromosome numbers of the backcross progenies were 24 to 48. seven yield-related traits of the parents, hybrid f(1), and backcross progenies were evaluated. simple sequence repeat markers a ... | 2009 | 20016972 |
| over-expression of mir172 causes loss of spikelet determinacy and floral organ abnormalities in rice (oryza sativa). | regulation of gene expression by micrornas (mirnas) plays a crucial role in many developmental and physiological processes in plants. mirnas act to repress expression of their target genes via mrna cleavage or translational repression. dozens of mirna families have been identified in rice, 21 of which are conserved between rice and arabidopsis. mir172 is a conserved mirna family which has been shown to regulate expression of apetala2 (ap2)-like transcription factors in arabidopsis and maize. the ... | 2009 | 20017947 |
| mef9, an e-subclass pentatricopeptide repeat protein, is required for an rna editing event in the nad7 transcript in mitochondria of arabidopsis. | rna editing in plants alters specific nucleotides from c to u in mrnas in plastids and in mitochondria. i here characterize the nuclear gene mitochondrial editing factor9 (mef9) that is required for rna editing of the site nad7-200 in the nad7 mitochondrial mrna in arabidopsis (arabidopsis thaliana). the mef9 protein belongs to the e subfamily of pentatricopeptide repeat proteins and unlike the three previously identified mitochondrial editing factors mef1 and mef11 in arabidopsis and ogr1 in ri ... | 2010 | 20018598 |
| association analysis of the amino acid contents in rice. | the main objective of the present study was to identify simple sequence repeat (ssr) markers associated with the amino acid content of rice (oryza sativa l.). ssr markers were selected by prescreening for the relationship to amino acid content. eighty-four rice landrace accessions from korea were evaluated for 16 kinds of amino acids in brown rice and genotyped with 25 ssr markers. analysis of population structure revealed four subgroups in the population. linkage disequilibrium (ld) patterns an ... | 2009 | 20021560 |
| mechanisms of microrna-mediated auxin signaling inferred from the rice mutant osaxr. | auxin, known as the central hormone, plays essential roles in plant growth and development. in auxin signaling pathways, the tiny rna molecules, i.e., micrornas (mirnas), show their strong potential in modulating the auxin signal transduction. recently, we isolated a novel auxin resistant rice mutant osaxr (oryza sativa auxin resistant) that exhibited plethoric root defects. microarray experiments were carried out to investigate the expression patterns of both the mirnas and the protein-coding g ... | 2010 | 20023405 |
| orthologs of the class a4 heat shock transcription factor hsfa4a confer cadmium tolerance in wheat and rice. | cadmium (cd) is a widespread soil pollutant; thus, the underlying molecular controls of plant cd tolerance are of substantial interest. a screen for wheat (triticum aestivum) genes that confer cd tolerance to a cd hypersensitive yeast strain identified heat shock transcription factor a4a (hsfa4a). ta hsfa4a is most similar to the class a4 hsfs from monocots. the most closely related rice (oryza sativa) homolog, os hsfa4a, conferred cd tolerance in yeast, as did ta hsfa4a, but the second most clo ... | 2009 | 20028842 |
| development and application of gene-based markers for the major rice qtl phosphorus uptake 1. | marker-assisted breeding is a very useful tool for breeders but still lags behind its potential because information on the effect of quantitative trait loci (qtls) in different genetic backgrounds and ideal molecular markers are unavailable. here, we report on some first steps toward the validation and application of the major rice qtl phosphate uptake 1 (pup1) that confers tolerance of phosphorus (p) deficiency in rice (oryza sativa l.). based on the pup1 genomic sequence of the tolerant donor ... | 2010 | 20035315 |
| apc-targeted raa1 degradation mediates the cell cycle and root development in plants. | protein degradation by the ubiquitin-proteasome system is necessary for a normal cell cycle. as compared with knowledge of the mechanism in animals and yeast, that in plants is less known. here we summarize research into the regulatory mechanism of protein degradation in the cell cycle in plants. anaphase-promoting complex/cyclosome (apc), in the e3 family of enzymes, plays an important role in maintaining normal mitosis. apc activation and substrate specificity is determined by its activators, ... | 2010 | 20037474 |
| identity, diversity, and molecular phylogeny of the endophytic mycobiota in the roots of rare wild rice (oryza granulate) from a nature reserve in yunnan, china. | rice (oryza sativa l.) is, on a global scale, one of the most important food crops. although endophytic fungi and bacteria associated with rice have been investigated, little is known about the endophytic fungi of wild rice (oryza granulate) in china. here we studied the root endophytic mycobiota residing in roots of o. granulate by the use of an integrated approach consisting of microscopy, cultivation, ecological indices, and direct pcr. microscopy confirmed the ubiquitousness of dark septate ... | 2010 | 20038691 |
| the agl6-like gene osmads6 regulates floral organ and meristem identities in rice. | although agamous-like6 (agl6) mads-box genes are ancient with wide distributions in gymnosperms and angiosperms, their functions remain poorly understood. here, we show the biological role of the agl6-like gene, osmads6, in specifying floral organ and meristem identities in rice (oryza sativa l.). osmads6 was strongly expressed in the floral meristem at early stages. subsequently, osmads6 transcripts were mainly detectable in paleas, lodicules, carpels and the integument of ovule, as well as in ... | 2010 | 20038961 |
| cadmium content of commercial and contaminated rice, oryza sativa, in thailand and potential health implications. | thailand is the number one global exporter and among the top five producers of rice in the world. a significant increase in anthropogenic contamination in agricultural soils over the past few decades has lead to concerns with cadmium and its uptake in rice. the cadmium levels in thai rice from different sources/areas were determined and used to estimate the potential health risks to consumers. the cadmium concentration in the commercial rice samples ranged from below the detection limit to 0.016 ... | 2010 | 20039016 |
| the bzip transcription factor osabf1 is an aba responsive element binding factor that enhances abiotic stress signaling in rice. | a number of basic leucine zipper (bzip) transcription factors are known to function in stress signaling in plants but few have thus far been functionally characterized in rice. in our current study in rice, we have newly isolated and characterized the osabf1 (oryza sativa aba responsive element binding factor 1) gene that encodes a bzip transcription factor. its expression in seedling shoots and roots was found to be induced by various abiotic stress treatments such as anoxia, salinity, drought, ... | 2010 | 20039193 |
| aco1, a gene for aminocyclopropane-1-carboxylate oxidase: effects on internode elongation at the heading stage in rice. | although reports on a gene for 1-amino-cyclopropane-1-carboxylate (acc) oxidase (aco1) in rice (oryza sativa l.) suggest that high levels of its transcript are associated with internode elongation of deep-water rice during submergence, the role of aco1 in rice development is largely unknown. the tissue-specificity of aco1 expression indicated that its transcript significantly accumulated in lower parts of elongating internodes at the heading stage. histochemical analysis and in situ hybridizatio ... | 2010 | 20040065 |
| arsenic accumulation and phosphorus status in two rice (oryza sativa l.) cultivars surveyed from fields in south china. | the consumption of paddy rice (oryza sativa l.) is a major inorganic arsenic exposure pathway in s.e. asia. a multi-location survey was undertaken in guangdong province, south china to assess arsenic accumulation and speciation in 2 rice cultivars, one an indica and the other a hybrid indica. the results showed that arsenic concentrations in rice tissue increased in the order grain < husk < straw < root. rice grain arsenic content of 2 rice cultivars was significant different and correlated with ... | 2010 | 20045585 |
| complete genomic structure of the cultivated rice endophyte azospirillum sp. b510. | we determined the nucleotide sequence of the entire genome of a diazotrophic endophyte, azospirillum sp. b510. strain b510 is an endophytic bacterium isolated from stems of rice plants (oryza sativa cv. nipponbare). the genome of b510 consisted of a single chromosome (3,311,395 bp) and six plasmids, designated as pab510a (1,455,109 bp), pab510b (723,779 bp), pab510c (681,723 bp), pab510d (628,837 bp), pab510e (537,299 bp), and pab510f (261,596 bp). the chromosome bears 2893 potential protein-enc ... | 2010 | 20047946 |
| seasonal losses of dissolved organic carbon and total dissolved solids from rice production systems in northern california. | water quality concerns have arisen related to rice (oryza sativa l.) field drain water, which has the potential to contribute large amounts of dissolved organic carbon (doc) and total dissolved solids (tds) to the sacramento river. field-scale losses of doc or tds have yet to be quantified. the objectives of this study were to evaluate the seasonal concentrations of doc and tds in rice field drain water and irrigation canals, quantify seasonal fluxes and flow-weighted (fw) concentrations of doc ... | 2015 | 20048318 |
| unintended compositional changes in transgenic rice seeds ( oryza sativa l.) studied by spectral and chromatographic analysis coupled with chemometrics methods. | unintended compositional changes in transgenic rice seeds were studied by near-infrared reflectance, gc-ms, hplc, and icp-aes coupled with chemometrics strategies. three kinds of transgenic rice with resistance to fungal diseases or insect pests were comparatively studied with the nontransgenic counterparts in terms of key nutrients such as protein, amino acids, fatty acids, vitamins, elements, and antinutrient phytic acid recommended by the organization for economic co-operation and development ... | 2010 | 20050687 |
| purification and biochemical characterization of recombinant rice udp-arabinopyranose mutase generated in insect cells. | plants utilize udp-arabinofuranose (udp-araf) in the biosynthesis of araf-containing complex carbohydrates. udp-araf is synthesized from udp-arabinopyranose by udp-arabinopyranose mutases (uams). here we describe the heterologous expression of rice (oryza sativa) uam genes in insect cells and report some of their enzymatic properties. recombinant uams might serve as useful tools for the biosynthesis of udp-araf and might be better than chemical synthesis. | 2010 | 20057139 |
| presence of three mycorrhizal genes in the common ancestor of land plants suggests a key role of mycorrhizas in the colonization of land by plants. | *the colonization of land by plants fundamentally altered environmental conditions on earth. plant-mycorrhizal fungus symbiosis likely played a key role in this process by assisting plants to absorb water and nutrients from soil. *here, in a diverse set of land plants, we investigated the evolutionary histories and functional conservation of three genes required for mycorrhiza formation in legumes and rice (oryza sativa), dmi1, dmi3 and ipd3. *the genes were isolated from nearly all major plant ... | 2010 | 20059702 |
| fine mapping of a gene for low-tiller number, ltn, in japonica rice (oryza sativa l.) variety aikawa 1. | tillering is one of the most important agronomic traits related to grain production in rice (oryza sativa l.). a japonica-type variety, aikawa 1, is known to have low-tiller number. the detailed location of a low-tillering gene, ltn, which has been localized on chromosome 8 in aikawa 1, was confirmed by molecular mapping. using bc5f2 individuals derived from a cross between ir64 and aikawa 1, the low-tillering gene was mapped to an interval defined by ssr markers ssr5816-3 and a4765. this was de ... | 2010 | 20062964 |
| identification of a rice stripe necrosis virus resistance locus and yield component qtls using oryza sativa x o. glaberrima introgression lines. | developing new population types based on interspecific introgressions has been suggested by several authors to facilitate the discovery of novel allelic sources for traits of agronomic importance. chromosome segment substitution lines from interspecific crosses represent a powerful and useful genetic resource for qtl detection and breeding programs. | 2010 | 20064202 |
| agricultural wetlands as potential hotspots for mercury bioaccumulation: experimental evidence using caged fish. | wetlands provide numerous ecosystem services, but also can be sources of methylmercury (mehg) production and export. rice agricultural wetlands in particular may be important sites for mehg bioaccumulation due to their worldwide ubiquity, periodic flooding schedules, and high use by wildlife. we assessed mehg bioaccumulation within agricultural and perennial wetlands common to california's central valley during summer, when the majority of wetland habitats are shallowly flooded rice fields. we i ... | 2010 | 20067279 |
| the wus homeobox-containing (wox) protein family. | the wox genes form a plant-specific subclade of the eukaryotic homeobox transcription factor superfamily, which is characterized by the presence of a conserved dna-binding homeodomain. the analysis of wox gene expression and function shows that wox family members fulfill specialized functions in key developmental processes in plants, such as embryonic patterning, stem-cell maintenance and organ formation. these functions can be related to either promotion of cell division activity and/or prevent ... | 2009 | 20067590 |
| gynumella flava gen. nov., sp. nov., isolated from the rhizosphere of rice (oryza sativa l.) managed under no-tillage practice. | 2010 | 20068238 | |
| gene expression profiles deciphering rice phenotypic variation between nipponbare (japonica) and 93-11 (indica) during oxidative stress. | rice is a very important food staple that feeds more than half the world's population. two major asian cultivated rice (oryza sativa l.) subspecies, japonica and indica, show significant phenotypic variation in their stress responses. however, the molecular mechanisms underlying this phenotypic variation are still largely unknown. a common link among different stresses is that they produce an oxidative burst and result in an increase of reactive oxygen species (ros). in this study, methyl violog ... | 2010 | 20072620 |
| mitochondrial gene in the nuclear genome induces reproductive barrier in rice. | hybrid incompatibility in f(1) hybrids or later generations is often observed as sterility or inviability. this incompatibility acts as postzygotic reproductive isolation, which results in the irreversible divergence of species. here, we show that the reciprocal loss of duplicated genes encoding mitochondrial ribosomal protein l27 causes hybrid pollen sterility in f(1) hybrids of the cultivated rice oryza sativa and its wild relative o. glumaepatula. functional analysis revealed that this gene i ... | 2010 | 20080642 |
| overexpression of osta-sir2141 caused abnormal polarity establishment and retarded growth in rice. | small rnas (smrnas) including mirnas and sirnas are critical for gene regulation and plant development. among the highly diverse sirnas, trans-acting sirnas (ta-sirnas) have been shown to be plant-specific. in arabidopsis, eight tas loci belonging to four families (tas1, tas2, tas3, and tas4) have been identified, and bioinformatics analysis reveals that the sequence of tas3 is highly conserved in plants. in this study, the function of tas3 ta-sirna (tasir-arf) has been revealed in rice (oryza s ... | 2010 | 20080824 |
| characterization of the rice pho1 gene family reveals a key role for ospho1;2 in phosphate homeostasis and the evolution of a distinct clade in dicotyledons. | phosphate homeostasis was studied in a monocotyledonous model plant through the characterization of the pho1 gene family in rice (oryza sativa). bioinformatics and phylogenetic analysis showed that the rice genome has three pho1 homologs, which cluster with the arabidopsis (arabidopsis thaliana) atpho1 and atpho1;h1, the only two genes known to be involved in root-to-shoot transfer of phosphate. in contrast to the arabidopsis pho1 gene family, all three rice pho1 genes have a cis-natural antisen ... | 2010 | 20081045 |
| global epigenetic and transcriptional trends among two rice subspecies and their reciprocal hybrids. | the behavior of transcriptomes and epigenomes in hybrids of heterotic parents is of fundamental interest. here, we report highly integrated maps of the epigenome, mrna, and small rna transcriptomes of two rice (oryza sativa) subspecies and their reciprocal hybrids. we found that gene activity was correlated with dna methylation and both active and repressive histone modifications in transcribed regions. differential epigenetic modifications correlated with changes in transcript levels among hybr ... | 2010 | 20086188 |
| cytochrome p450 family member cyp704b2 catalyzes the {omega}-hydroxylation of fatty acids and is required for anther cutin biosynthesis and pollen exine formation in rice. | the anther cuticle and microspore exine act as protective barriers for the male gametophyte and pollen grain, but relatively little is known about the mechanisms underlying the biosynthesis of the monomers of which they are composed. we report here the isolation and characterization of a rice (oryza sativa) male sterile mutant, cyp704b2, which exhibits a swollen sporophytic tapetal layer, aborted pollen grains without detectable exine, and undeveloped anther cuticle. in addition, chemical compos ... | 2010 | 20086189 |
| zinc finger-containing glycine-rich rna-binding protein in oryza sativa has an rna chaperone activity under cold stress conditions. | the rice (oryza sativa) genome harbours three genes encoding cyscyshiscys (cchc)-type zinc finger-containing glycine-rich rna-binding proteins, designated osrz proteins, but their importance and physiological functions remain largely unknown. here, the stress-responsive expression patterns of osrzs were assessed, and the biological and cellular functions of osrzs were evaluated under low temperature conditions. the expression levels of the three osrzs were up-regulated by cold stress, whereas dr ... | 2010 | 20088860 |
| a candidate gene osapc6 of anaphase-promoting complex of rice identified through t-dna insertion. | a dwarf mutant (oryza sativa anaphase-promoting complex 6 (osapc6)) of rice cultivar basmati 370 with 50% reduced plant height as compared to the wild type was isolated by agrobacterium tumefaciens-mediated transformation using hm(r) ds cassette. this mutant was found to be insensitive to exogenous gibberellic acid (ga(3)) application. homozygous mutant plants showed incomplete penetrance and variable expressivity for plant height and pleiotropic effects including gibberellic acid insensitivity, ... | 2010 | 20091079 |
| cross-kingdom comparison of transcriptomic adjustments to low-oxygen stress highlights conserved and plant-specific responses. | high-throughput technology has facilitated genome-scale analyses of transcriptomic adjustments in response to environmental perturbations with an oxygen deprivation component, such as transient hypoxia or anoxia, root waterlogging, or complete submergence. we showed previously that arabidopsis (arabidopsis thaliana) seedlings elevate the levels of hundreds of transcripts, including a core group of 49 genes that are prioritized for translation across cell types of both shoots and roots. to recogn ... | 2010 | 20097791 |
| genome-wide analysis of ethylene-responsive element binding factor-associated amphiphilic repression motif-containing transcriptional regulators in arabidopsis. | the ethylene-responsive element binding factor-associated amphiphilic repression (ear) motif is a transcriptional regulatory motif identified in members of the ethylene-responsive element binding factor, c2h2, and auxin/indole-3-acetic acid families of transcriptional regulators. sequence comparison of the core ear motif sites from these proteins revealed two distinct conservation patterns: lxlxl and dlnxxp. proteins containing these motifs play key roles in diverse biological functions by negat ... | 2010 | 20097792 |
| dynamics of periarbuscular membranes visualized with a fluorescent phosphate transporter in arbuscular mycorrhizal roots of rice. | in arbuscular mycorrhizal (am) symbiosis, host plants supply photosynthates to am fungi and, in return, they receive inorganic nutrients such as phosphate from finely branched fungal arbuscules. plant cortical cells envelope arbuscules with periarbuscular membranes that are continuous with the plant plasma membranes. we prepared transgenic rice (oryza sativa) plants that express a fusion of green fluorescent protein with rice am-inducible phosphate transporter, ospt11-gfp, and grew them with am ... | 2010 | 20097910 |
| response to zinc deficiency of two rice lines with contrasting tolerance is determined by root growth maintenance and organic acid exudation rates, and not by zinc-transporter activity. | *zinc (zn)-deficient soils constrain rice (oryza sativa) production and cause zn malnutrition. the identification of zn-deficiency-tolerant rice lines indicates that breeding might overcome these constraints. here, we seek to identify processes underlying zn-deficiency tolerance in rice at the physiological and transcriptional levels. *a zn-deficiency-tolerant line ril46 acquires zn more efficiently and produces more biomass than its nontolerant maternal line (ir74) at low [zn](ext) under field ... | 2010 | 20100202 |
| the submergence tolerance regulator sub1a mediates stress-responsive expression of ap2/erf transcription factors. | we previously characterized the rice (oryza sativa) submergence1 (sub1) locus encoding three ethylene-responsive factor (erf) transcriptional regulators. genotypes carrying the sub1a-1 allele are tolerant of prolonged submergence. to elucidate the mechanism of sub1a-1-mediated tolerance, we performed transcriptome analyses comparing the temporal submergence response of sub1a-1-containing tolerant m202(sub1) with the intolerant isoline m202 lacking this gene. we identified 898 genes displaying su ... | 2010 | 20107022 |
| imaging and analysis platform for automatic phenotyping and trait ranking of plant root systems. | the ability to nondestructively image and automatically phenotype complex root systems, like those of rice (oryza sativa), is fundamental to identifying genes underlying root system architecture (rsa). although root systems are central to plant fitness, identifying genes responsible for rsa remains an underexplored opportunity for crop improvement. here we describe a nondestructive imaging and analysis system for automated phenotyping and trait ranking of rsa. using this system, we image rice ro ... | 2010 | 20107024 |
| combined toxicity of copper and cadmium to six rice genotypes (oryza sativa l.). | accumulations of copper (cu) and cadmium (cd) in six rice cultivars (94d-22, 94d-54, 94d-64, gui630, yy-1, and ky1360) were evaluated through exposure to heavy metal contamination (100 mg/kg cu, 1.0 mg/kg cd, and 100 mg/kg cu + 1.0 mg/kg cd) in a greenhouse. the dry weights of shoot and root, concentrations of cu and cd in plant tissues and the cu, cd, p, fe concentrations in the root surface iron plaques were analyzed eight weeks later after treatment. the results indicated that the plant bioma ... | 2009 | 20108667 |
| rice metal-nicotianamine transporter, osysl2, is required for the long-distance transport of iron and manganese. | rice (oryza sativa) is indispensable in the diet of most of the world's population. thus, it is an important target in which to alter iron (fe) uptake and homeostasis, so as to increase fe accumulation in the grain. we previously isolated osysl2, a functional iron [fe(ii)]- and manganese [mn(ii)]-nicotianamine complex transporter that is expressed in phloem cells and developing seeds. we produced rnai (osysl2i) and overexpression lines (oxosysl2) of osysl2. at the vegetative stage in an osysl2i ... | 2010 | 20128878 |
| receptor-like kinase ossik1 improves drought and salt stress tolerance in rice (oryza sativa) plants. | receptor-like kinases (rlks) play essential roles in plant growth, development and responses to environmental stresses. a putative rlk gene, ossik1, with extracellular leucine-rich repeats was cloned and characterized in rice (oryza sativa). ossik1 exhibits kinase activity in the presence of mn(2+), and the ossik1 kinase domain has the ability to autophosphorylate and phosphorylate myelin basic protein (mbp). ossik1 promoter-gus analysis revealed that ossik1 is expressed mainly in the stem and s ... | 2010 | 20128882 |
| cytosolic apx knockdown indicates an ambiguous redox responses in rice. | ascorbate peroxidases (apx, ec 1.1.11.1) are class i heme-peroxidases, which catalyze the conversion of h(2)o(2) into h(2)o, using ascorbate as a specific electron donor. previously, the presence of eight apx genes was identified in the nuclear genome of rice (oryza sativa), encoding isoforms that are located in different sub-cellular compartments. herein, the generation of rice transgenic plants silenced for either both or each one of the cytosolic apx1 and apx2 genes was carried out in order t ... | 2010 | 20129631 |
| a novel mybs3-dependent pathway confers cold tolerance in rice. | rice (oryza sativa) seedlings are particularly sensitive to chilling in early spring in temperate and subtropical zones and in high-elevation areas. improvement of chilling tolerance in rice may significantly increase rice production. mybs3 is a single dna-binding repeat myb transcription factor previously shown to mediate sugar signaling in rice. in this study, we observed that mybs3 also plays a critical role in cold adaptation in rice. gain- and loss-of-function analyses indicated that mybs3 ... | 2010 | 20130099 |
| abscisic acid-induced resistance against the brown spot pathogen cochliobolus miyabeanus in rice involves map kinase-mediated repression of ethylene signaling. | the plant hormone abscisic acid (aba) is involved in an array of plant processes, including the regulation of gene expression during adaptive responses to various environmental cues. apart from its well-established role in abiotic stress adaptation, emerging evidence indicates that aba is also prominently involved in the regulation and integration of pathogen defense responses. here, we demonstrate that exogenously administered aba enhances basal resistance of rice (oryza sativa) against the bro ... | 2010 | 20130100 |
| virulence analysis and gene expression profiling of the pigment-deficient mutant of xanthomonas oryzae pathovar oryzae. | xanthomonas oryzae pathovar oryzae (xoo) causes bacterial blight disease in rice (oryza sativa l.). for a study of function, we constructed a random insertion mutant library of xoo using a tn5 transposon and isolated the mutant strain (m11; arok::tn5) that had extremely low pigment production. in addition, m11 had decreased virulence against the susceptible rice cultivar ir24. thermal asymmetric interlaced-pcr and sequence analysis of m11 revealed that the transposon was inserted into the arok g ... | 2009 | 20132309 |
| over-expression of osa-mir396c decreases salt and alkali stress tolerance. | salt and alkali stress are two of the main environmental factors limiting rice production. thus, understanding the mechanisms of salinity and alkali stress tolerance is necessary to modify rice to increase its resistance to salinity and alkaline stress. micrornas (mirnas) are approximately 21-nucleotide rnas that are ubiquitous regulators of gene expression in eukaryotic organisms. in plants, mirnas constitute one of five classes of small rnas that function primarily as negative regulators for g ... | 2010 | 20135324 |
| quantitative analysis of plant polyamines including thermospermine during growth and salinity stress. | arabidopsis thaliana was thought to contain two spermine synthase genes, acaulis 5 (acl5) and spms. recent investigations, however, revealed that the acl5 gene encodes thermospermine synthase. in this study, we have established a simple method to separate two isomers of tetraamine, spermine and thermospermine, in extracts from plant tissues of less than 500 mg. polyamines (pas) extracted from plant tissues were benzoylated, and the derivatives were completely resolved by high-performance liquid ... | 2010 | 20137962 |
| microrna-mediated signaling involved in plant root development. | microrna (mirna), recently recognized as a critical post-transcriptional modulator of gene expression, is involved in numerous biological processes in both animals and plants. although eudicots and monocots, such as the model plants arabidopsis (arabidopsis thaliana) and rice (oryza sativa), possess distinct root systems, several homologous mirna families are reported to be involved in root growth control in both plants. consistent with recent notion that numerous signaling pathways are implicat ... | 2010 | 20138828 |
| gene cloning, bacterial expression, and purification of a novel rice (oryza sativa l.) ubiquitin-related protein, rurm1. | a rice ubiquitin-related modifier-1 (rurm1) gene was cloned and transformed in escherichia coli. we successfully expressed the rurm1 protein as a glutathione s-transferase (gst)-fusion protein by cultivating the e. coli cells at 16 degrees c for 16 h. after cleavage of gst, we obtained a single protein of 12 kda. this protein was identified as the rurm1 protein by western blot analysis. | 2010 | 20139593 |
| genetic analysis and mapping of a thermosensitive genic male sterility gene, tms6(t), in rice (oryza sativa l.). | thermosensitive genic male-sterile (tgms) rice is widely used in hybrid rice production. because of its specific temperature requirement, it can be used only in a narrow rice-growing zone in asia. a newly discovered tgms line, g20s, has an opposite phenotype compared with normal tgms lines. g20s is completely sterile when the temperature is lower than 29.5 degrees c. thus, it can be widely used in a larger area. genetic analysis indicated that the sterility of g20s was controlled by a single rec ... | 2010 | 20140030 |
| effect of anthocyanin-rich extract from black rice (oryza sativa l. japonica) on chronically alcohol-induced liver damage in rats. | the study evaluated the protective effect of anthocyanin-rich extract from black rice (aebr) on chronic ethanol-induced biochemical changes in male wistar rats. administration of ethanol (3.7 g/kg/day) to wistar rats for 45 days induced liver damage with a significant increase (p < 0.05) of aspartate transaminase (ast), alanine transaminase (alt), gamma glutamyl transferase (ggt) in the serum and the hepatic malondialdehyde (mda) level. in contrast, administration of aebr (500 mg/kg) along with ... | 2010 | 20143824 |
| the decline in ascorbic acid content is associated with cadmium toxicity of rice seedlings. | cadmium (cd) toxicity of rice (oryza sativa l. cv. taichung native 1) seedlings was evaluated by the decrease in chlorophyll content and the increase in malondialdehyde (mda) in the second leaves of rice seedlings. cdcl2 (5 microm) treatment was accompanied by a decrease in the contents of ascorbic acid (asa) and asa + dehydroascorbate (dha) and in the ratios of asa/dha in leaves. however, cdcl2 treatment resulted in an increase in dha content in leaves. moreover, the decrease in asa content was ... | 2010 | 20144872 |
| identification and analysis of in planta expressed genes of magnaporthe oryzae. | infection of plants by pathogens and the subsequent disease development involves substantial changes in the biochemistry and physiology of both partners. analysis of genes that are expressed during these interactions represents a powerful strategy to obtain insights into the molecular events underlying these changes. we have employed expressed sequence tag (est) analysis to identify rice genes involved in defense responses against infection by the blast fungus magnaporthe oryzae and fungal genes ... | 2010 | 20146797 |
| maize (zea mays): a model organism for basic and applied research in plant biology. | zea mays ssp. mays is one of the world's most important crop plants, boasting a multibillion dollar annual revenue. in addition to its agronomic importance, maize has been a keystone model organism for basic research for nearly a century. within the cereals, which include other plant model species such as rice (oryza sativa), sorghum (sorghum bicolor), wheat (triticum spp.), and barley (hordeum vulgare), maize is the most thoroughly researched genetic system. several attributes of the maize plan ... | 0 | 20147033 |
| the application of restriction landmark genome scanning method for surveillance of non-mendelian inheritance in f(1) hybrids. | we analyzed inheritance of dna methylation in reciprocal f(1) hybrids (subsp. japonica cv. nipponbare x subsp. indica cv. kasalath) of rice (oryza sativa l.) using restriction landmark genome scanning (rlgs), and detected differing rlgs spots between the parents and reciprocal f(1) hybrids. mspi/hpaii restriction sites in the dna from these different spots were suspected to be heterozygously methylated in the nipponbare parent. these spots segregated in f(1) plants, but did not segregate in self ... | 2009 | 20148066 |
| hybrid sterility in plant: stories from rice. | hybrid sterility is the most common form of postzygotic reproductive isolation in plants. the best-known example is perhaps the hybrid sterility between indica and japonica subspecies of asian cultivated rice (oryza sativa l.). major progress has been reported recently in rice in identifying and cloning hybrid sterility genes at two loci regulating female and male fertility, respectively. genetic analyses and molecular characterization of these genes, together with the results from other model o ... | 2010 | 20153244 |
| metabolome and photochemical analysis of rice plants overexpressing arabidopsis nad kinase gene. | the chloroplastic nad kinase (nadk2) is reported to stimulate carbon and nitrogen assimilation in arabidopsis (arabidopsis thaliana), which is vulnerable to high light. since rice (oryza sativa) is a monocotyledonous plant that can adapt to high light, we studied the effects of nadk2 expression in rice by developing transgenic rice plants that constitutively expressed the arabidopsis chloroplastic nadk gene (nk2 lines). nk2 lines showed enhanced activity of nadk and accumulation of the nadp(h) p ... | 2010 | 20154096 |