Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| [clostridium perfringens sepsis following criminal abortion]. | 1982 | 6281674 | |
| [gas phlegmon of the neck and chest (author's transl)]. | 1981 | 6281714 | |
| [clostridium perfringens in meat products in chile: contamination level and enteric toxicity (author's transl)]. | 1981 | 6281871 | |
| [dry medium for isolating clostridium perfringens]. | the possibility of using blood substitutes (hydrolysin, casein hydrolysate "tsolipk" and amino peptide) with expired shelf life as a culture medium for the isolation of cl. perfringens has been studied. dry culture medium based on these inedible products has been developed. to stimulate bacterial growth, fodder yeast extract has been used. the suppression of the growth of extraneous microflora is ensured by the use of antibiotics: polymyxin sulfate and mycerin sulfate. the recommended medium is ... | 1982 | 6282016 |
| human neutrophil peptide receptors: mobilization mediated by phospholipase c. | incubation of human neutrophils with phospholipase c from clostridium perfringens caused an increase in the ability of the treated cells to bind the chemotactic peptide, f-met-leu-phe. the increase in binding was related to an increase in specific binding of the ligand. the increase in specific binding was, in turn, related to an increased number of peptide receptors. the dissociation constant (kd) for the tripeptide was not altered, on the average, by enzyme treatment. the increase in peptide r ... | 1982 | 6282127 |
| effects of cholesterol evulsion on susceptibility to perfringolysin o of human erythrocytes. | human erythrocytes preincubated with a phosphatidylcholine suspension (preincubated cells) showed decreased susceptibility to perfringolysin o, the decrease being strongly affected by preincubation time and temperature, and the phosphatidyl choline concentration. the binding of the toxin to the preincubated cells also decreased with the preincubation time and reached minimum at 37 degrees c for 6 h. through this preincubation, about 30% of cholesterol was removed from cells without lysis. the su ... | 1982 | 6282326 |
| interactions of dna with divalent metal ions. iv. competitive studies of mn2+ binding to at- and gc-rich dnas. | 1982 | 6282356 | |
| acute gastric dilation and rupture in macaca arctoides associated with clostridium perfringens. | a clinical case of gastric dilation and rupture is described in an adult male macaca arctoides. clostridium perfringens was isolated from the heart blood and liver. data collected from our macaque colony for 13 years indicated a mortality rate of 3.5% due to acute dilation. | 1981 | 6283084 |
| microbiology of ova harvested from commercially slaughtered mature chickens. | the microbiological quality of ova harvested from mature chickens in commercial slaughter plants was studied. greatest contamination of the product occurred at the location where ova were drawn from the carcasses. water fluming and chilling procedures caused some reduction in contamination levels. total aerobic plate counts, coagulase-positive staphylococci, presumptive clostridium perfringens and enterobacteriaceae counts of chilled ova were low, and no salmonellae were detected on these sample ... | 1982 | 6283510 |
| [emphysematous cholecystitis. report of 2 cases]. | 1982 | 6283608 | |
| tampon associated toxic shock syndrome. | 1982 | 6283629 | |
| [method of determining the biochemical behavior of clostridia]. | a fast and readily applicable method is suggested for the determination of the biochemical reactions of the spore producing anaerobic organisms. a pepton coloid medium with the addition of 0.15 per cent agar-agar was used as a basic nutrient medium. the cultivation of bacteria did not require any special conditions. reading of the results was carried out with the addition of 0.1 per cent phenol red after 48 hours of incubation of the cultures. this method showed a number of advantages over the m ... | 1981 | 6283724 |
| immune regulatory factors in sheep fetuses. | after an antigenic stimulus in vitro, a fraction was found in the supernatant fluids of spleen (s-1) and thymus (t-1) cell cultures of 70 to 80-day-old sheep fetuses infected with ovine adenovirus in utero. the fraction agreed with alpha-1-fetoprotein in mobility and its molecular weight was 67 000. injected into adult sheep, s-1 and t-1 had a suppressive effect on the humoral immune response to both adenovirus and clostridium perfringens d vaccine. tests with peripheral lymphocytes suggested th ... | 1982 | 6283803 |
| a preliminary study on the pathogenicity of axenically grown entamoeba histolytica for the golden hamster. | 1982 | 6284104 | |
| regulation of fatty acid composition of cl. perfringens type a cells by hydrogen and hydroxyl ions. | the composition of the fatty acid profiles of cl. perfringens type a, grown on media with initial ph values from 5.5 to 9.0, was determined by the method of gas-liquid chromatography. the fatty acid profiles are stabilized in 18- to 24-h cultures. hydrogen ions stimulate the synthesis of cyclopropane carboxylic fatty acids and "desaturase" activity; hydroxyl ions inhibit these processes. the content of saturated fatty acids in the bacteria is regulated by the initial acid-alkaline conditions of ... | 1981 | 6284259 |
| percutaneous fixation of left displaced abomasum, using a bar suture. | 1982 | 6284690 | |
| a comparison of the levels of faecal indicator bacteria in water and human faeces in a rural area of a tropical developing country (sierra leone). | the levels of faecal coliforms (fc), indole-positive fc (presumptive escherichia coli), faecal streptococci (fs), streptococcus faecalis and clostridium perfringens in the natural water sources used by 29 rural settlements in sierra leone were investigated. levels of the same indicators in human faeces were also investigated. the incidence of salmonella spp. in both habitats and the temperature, ph and conductivity of water sources were also recorded. all water sources were contaminated with the ... | 1982 | 6284838 |
| [fate of clostridium perfringens in intestine of guinea pigs after oral ingestion (author's transl)]. | 1981 | 6285030 | |
| implication of clostridium difficile and clostridium perfringens iota toxins in experimental lincomycin-associated colitis of rabbits. | following oral administration of lincomycin, over 50% of two groups of 12 rabbits each died between 4 and 56 days with distended, non-hemorrhagic, fluid-filled ceca. bacteria-free cecal filtrates from the rabbits that died were lethal for mice, cytopathic in y-1 tissue culture monolayers, and caused increased vascular permeability in rabbit skin. although the cecal filtrates of both groups had similar biological activity, the filtrate activity of one group was neutralized by clostridium perfring ... | 1982 | 6285077 |
| acute infection of a mediastinal cyst. | 1982 | 6285437 | |
| fatal intravascular hemolysis associated with t-polyagglutination. | 1982 | 6285558 | |
| [colony morphology of clostridium perfringens type a (author's transl)]. | at least 4 different colony form of clostridium perfringens on the surface of yeast extract blood agar after overnight incubation were demonstrated. a: glossy colonies with entire margins, mostly surrounded by narrow zones of complete hemolysis and a much wider zone of incomplete hemolysis (colonies typical for c. perfringens (fig. 1), b: dwarf colonies, similar to a, but very small (fig. 2), c:rough colonies slightly raised with lobate margins (fig. 3-4), d: flat colonies with irregular surface ... | 1982 | 6285643 |
| [antagonistic effect against "clostridium perfringens" exerted in the digestive tract of gnotobiotic mice by "clostridium" strains isolated from the microflora of conventional mice (author's transl)]. | bacterial strains isolated from the digestive tract of conventional mice produced a barrier effect against a strain of clostridium perfringens type a (strain cp) in the gastrointestinal tract of gnotobiotic mice. this barrier effect was observed when mice, monoassociated with escherichia coli k12, were inoculated with a mixed culture of fusiform-shaped clostridia. the collection of fusiforms (collection d) was obtained from a single colony chosen from among the 26 types studied. collection d and ... | 1982 | 6285793 |
| capillary agar diffusion assay for measuring metronidazole in human gingival crevice fluid. | an agar diffusion assay in capillaries, with fusobacterium nucleatum m2 as the indicator strain, has been developed to measure metronidazole concentrations in gingival crevice fluid. the assay allows the measurement of 1.9 micrograms of metronidazole per ml in sample amounts of 0.6 microliters. | 1982 | 6285816 |
| wet and dry bacterial spore densities determined by buoyant sedimentation. | the wet densities of various types of dormant bacterial spores and reference particles were determined by centrifugal buoyant sedimentation in density gradient solutions of three commercial media of high chemical density. with metrizamide or renografin, the wet density values for the spores and permeable sephadex beads were higher than those obtained by a reference direct mass method, and some spore populations were separated into several density bands. with percoll, all of the wet density value ... | 1982 | 6285824 |
| reactivity of glycoconjugates in membranes. i. determination of transbilayer distribution of gangliosides in lipid vesicles by chemical methods. | two simple chemical methods are described for the determination of the transbilayer distribution of gangliosides gd1a and gm1 in phosphatidylcholine vesicles. the data presented here show an increase in the percentage of gd1a exposed on the outer surface of vesicles with increasing mole fraction of gd1a. the percentage of gd1a exposed on 1:50 and 1:5 gd1a-dipalmitoylphosphatidylcholine vesicles were found to be 67% and 83%, respectively. the same trend is seen for vesicles with dimyristoylphosph ... | 1982 | 6285968 |
| gas gangrene following perforation of the colon. | 1982 | 6286082 | |
| gram-positive bacteria-induced granulocytopenia and pulmonary leukostasis in rabbits. | pneumococci have been shown to induce granulocytopenia and pulmonary leukostasis which might contribute to morbidity and mortality in pneumococcal sepsis. we studied whether other gram-positive species (groups a and b streptococci, staphylococcus aureus, bacillus cereus, and clostridium perfringens) could also induce these phenomena. rabbits were challenged with nonviable preparations of each species, and all five species induced profound granulocytopenia (mean decrease of 90%) and pulmonary leu ... | 1982 | 6286493 |
| [antibiotic resistance of anaerobic bacteria (author's transl)]. | reference data on the overall resistance patterns of anaerobic bacteria are needed since in individual clinical cases susceptibility testing is complicated by the long generation time and difficulties of test conditions. benzylpenicillin and tetracyclines are the drugs of choice against clostridium perfringens. newer cephalosporins are also active, whereas other clostridium species have been found resistant. beta-lactam antibiotics show high activity against peptococcaceae; resistance to clindam ... | 1982 | 6286503 |
| partial purification and properties of phosphatidylserine synthase from clostridium perfringens. | the membrane-associated phospholipid biosynthetic enzyme cytidine 5'-diphospho-1,2-diacyl-sn-glycerol:l-serine o-phosphatidyltransferase (phosphatidylserine synthase; ec 2.7.8.8) was partially purified 337-fold from a cell-free extract of the gram-positive pathogenic anaerobe clostridium perfringens (atcc 3624). the purification procedure included extraction from the cell envelope with the nonionic detergent triton x-100, followed by affinity chromatography on cytidine 5'-diphosphate-diacylglyce ... | 1982 | 6286597 |
| rapid and sensitive sialidase assay by high-performance liquid chromatography and its application to detection of sialidase in human urine. | 1982 | 6286699 | |
| a simple device for growing clostridium perfringens and its application in bacteriocin studies. | a simple device constructed of common laboratory material served as a minifermenter for the growth of clostridium perfringens. a constant flow of nitrogen gas into a culture tube containing c. perfringens assured agitation of the culture and a mechanism for dispensing small volumes of liquid from the culture without disturbing the growth environment. the method was applied to examining the growth-inhibiting effect of bacteriocins of c. perfringens where a very economical use of radioactive isoto ... | 1982 | 6288212 |
| quantitation of binding and subcellular distribution of clostridium perfringens enterotoxin in rat liver cells. | binding of enterotoxin from clostridium perfringens type a was studied in suspensions of parenchymal and nonparenchymal cells from rat liver. in hepatocytes, 1.5 x 10(6) specific binding sites per cell with an association constant of 3.2 x 10(6) m-1 were found. about 1% of the added toxin was nonspecifically bound to the hepatocytes. at concentrations of toxin below 0.1 micrograms/ml, 80% of the toxin density of 7 x 10(6) cells per ml. binding did not increase after the cells became permeable to ... | 1982 | 6288564 |
| purification of chlamydia trachomatis lymphogranuloma venereum elementary bodies and their interaction with hela cells. | a procedure has been developed to yield infectious elementary bodies of the lymphogranuloma venereum strains lgv 434 and 404 of chlamydia trachomatis, labelled during intracellular growth in hela 229 cells. the final preparation, obtained after velocity sedimentation of a polycarbonate membrane-filtered sample through a sucrose gradient, is free of host proteins and, more importantly, of chlamydial reticulate bodies. using such purified preparations, it was found that the association of lgv 434 ... | 1982 | 6288839 |
| implication of plasma free hemoglobin in massive clostridial hemolysis. | prolonged tissue oxygenation and maintenance of intravascular oncotic pressure are severely impaired with the absence of an effective rbc mass. we recently encountered a patient with a nondetectable hematocrit value (0%) attributed to clostridium perfringens hemolysis. the patient maintained normal bp, tissue oxygenation, and mentation and survived longer than four hours, despite ineffective transfusion therapy. plasma free hemoglobin was responsible for the preservation of tissue oxygenation, i ... | 1982 | 6288977 |
| [clinicomorphological characteristics of suppurative wounds treated with iruksol]. | 1982 | 6289004 | |
| methane and hydrogen production by human intestinal anaerobic bacteria. | the gas above liquid cultures of a variety of human intestinal anaerobic bacteria was sampled and analysed by headspace gas chromatography. hydrogen production was greatest with strains of the genus clostridium, intermediate with anaerobic cocci and least with bacteroides sp. very few strains produced methane although small amounts were detected with one strain of b. thetaiotaomicron, c. perfringens and c. histolyticum. there may be a relationship between these anaerobic bacteria and several gas ... | 1982 | 6289602 |
| [method of determining the antibiotic sensitivity of anaerobic microorganisms using standard disks]. | three variants of the procedure for determination of antibiotic sensitivity in anaerobic microorganisms with the use of standard paper discs were developed. according to the first variant the solid nutrient medium is melted at 46 degrees c and mixed with the culture of the microbe being tested. the mixture is added to the cover of a petri dish. when the medium becomes solid, antibiotic sensitivity discs are placed onto the agar surface. after that one more layer of the medium is added. the mediu ... | 1982 | 6289725 |
| macrolide-lincosamide-streptogramin resistance patterns in clostridium perfringens from animals. | different patterns of resistance against commonly used macrolide, lincosamide, and streptogramin antibiotics were found in clostridium perfringens of animal origin. the patterns were designated as (i) macrolide-lincosamide-streptogramin group b generalized resistance, (ii) macrolide-lincosamide generalized resistance, (iii) macrolide-lincosamide inducible resistance, and (iv) macrolide-lincosamide-streptogramin low-level generalized resistance. the strains of the fourth pattern were able to inac ... | 1981 | 6289728 |
| histamine-producing bacteria in decomposing skipjack tuna (katsuwonus pelamis). | spoilage in skipjack tuna (katsuwonus pelamis) was studied under controlled conditions by incubating whole, fresh fish in seawater at 38 degrees c, the optimum temperature for histamine formation. bacterial isolates were obtained from the loin tissue of a decomposing tuna containing 134 mg of histamine per 100 g and a total anaerobic count of 3.5 x 10(5)/g after incubation for 24 h. over 92% of the 134 isolates obtained were facultatively or obligately anaerobic bacteria. eighteen isolates produ ... | 1982 | 6289747 |
| intracellular antibody against clostridium perfringens enterotoxin fails to counteract toxin-induced damage. | 1982 | 6290086 | |
| newcastle disease virus contains a linkage-specific glycoprotein sialidase. application to the localization of sialic acid residues in n-linked oligosaccharides of alpha 1-acid glycoprotein. | newcastle disease virus sialidase was found to exhibit strict specificity for hydrolysis of the neuac alpha 2 leads to 3gal linkage contained in glycoprotein oligosaccharides both n-linked to asparagine and o-linked to threonine or serine under conditions that left oligosaccharides containing the neuac alpha 2 leads to 2 leads to 6gal and neuac alpha 2 leads to 6gallnac linkages intact. this was determined, in part, by examining the viral sialidase for its ability to hydrolyze glycoprotein oligo ... | 1982 | 6290480 |
| [anaerobic infection]. | 1982 | 6291242 | |
| adaptation of an automated microbiology system for the growth of anaerobes and performance of antimicrobial susceptibility tests. | the authors examined the feasibility of growing anaerobes in an automated microbiology system (ms-2) and using the system for antimicrobial susceptibility testing. a total of 78 of 100 clinical anaerobic isolates grew in the ms-2 if 100 microl of an undiluted overnight suspension of organisms was inoculated into at least 2.5 ml of freshly prepared wilkins-chalgren broth supplemented with 0.07% agar. susceptibility test results were determined with 50 anaerobes tested against seven antibiotics in ... | 1982 | 6291376 |
| effects of temperature, ph and detergents on the molecular conformation of the enterotoxin of clostridium perfringens. | the effects of temperature, ph and sodium dodecyl sulfate on the conformation of the enterotoxin from clostridium perfringens type a were followed by circular dichroism in both the peptide and aromatic regions. at near-physiological conditions (35 degrees c, ph 6.7) the enterotoxin exhibited a conformation consisting of approximately 60% pleated sheet, 40% non-periodic, and essentially no helix. the peptide region was relatively stable at temperatures up to 55 degrees c and at ph values ranging ... | 1982 | 6291615 |
| response of clostridium perfringens and its l form to bacteriocins of c. perfringens. | clostridium perfringens strain no. 28 and its penicillin-induced stable l form were treated with 10 different bacteriocins of c. perfringens. viable count and labelled amino acid incorporation experiments revealed that the l form was sensitive to two and possibly three bacteriocins to which the bacillus was not, while both forms were commonly sensitive to two other bacteriocins and resistant to five others. adsorption of bacteriocin, immunity factors, or perhaps uptake of bacteriocin might be pr ... | 1982 | 6291734 |
| clostridium perfringens type a enterotoxin: characterization of the amino-terminal region. | the amino-terminal region of the enterotoxin of clostridium perfringens was investigated by automated sequence analysis. the primary structure results revealed that the enterotoxin is composed of a single polypeptide amino acid sequence. computer comparison of a 20-residue sequence with a sequence library of reported proteins revealed no significant chemical similarities, indicating that the enterotoxin represents a unique polypeptide primary structure. | 1982 | 6292098 |
| purification of two clostridium perfringens enterotoxin-like proteins and their effects on membrane permeability in primary cultures of adult rat hepatocytes. | we isolated two proteins, et-1 and et-2, from the sporangial extracts of clostridium perfringens type a. both proteins had some properties in common with the well-known c. perfringens enterotoxin. et-1 and et-2 behaved as single and distinct entities in anion exchange chromatography and disk gel electrophoresis. et-2 was the more anionic protein since it eluted more slowly from the anion exchange column and migrated faster toward the anode in polyacrylamide disk gel electrophoresis (ph 8.5, nati ... | 1982 | 6292107 |
| [case of anaerobic infection]. | 1982 | 6292574 | |
| investigation of the effects of phospholipase c on human platelets: evidence that aggregation induced by phospholipase c is independent of prostaglandin generation, released adp and is modulated by cyclic amp. | effects and the mechanism of action of phospholipase c (plc), from clostridium perfringens, on washed human platelets were examined to better understand the role of plc in platelet function. plc caused aggregation and secretion of [14c]-5ht, without concomitant loss of cytoplasmic, ldh, in a concentration dependent manner. p-nitrophenylphosphorylcholine, a substrate for plc, blocked these responses in a concentration dependent manner. in other experiments hirudin, alpha-1-antitrypsin and soybean ... | 1982 | 6293114 |
| acute gastric dilatation in nonhuman primates: review and case studies. | acute gastric dilatation occurs sporadically in laboratory-housed nonhuman primates. clinical histories often include chronic drug administration, food restriction, accidental overfeeding, and prior anesthesia. monkeys may be found dead or may have clinical signs of colic, abdominal distention, and dyspnea. death in untreated cases is due to impaired venous return and cardiopulmonary failure. gastric distention with fermented gaseous ingesta and congestion of the abdominal viscera are the predom ... | 1982 | 6293147 |
| comparative effects of oral administration of trimethoprim/sulphadiazine or oxytetracycline on the faecal flora of horses. | a study was carried out on the bacteriological faecal flora of horses before and after oral doses of oxytetracycline or trimethoprim plus sulphadiazine. administration of oxytetracycline was rapidly followed by large increases in counts of coliforms. bacteroides and streptococcus species, the disappearance of veillonella species, the appearance of clostridium perfringens type a in large numbers and the accumulation of watery fluid in the rectal contents. these changes were not seen following adm ... | 1982 | 6293150 |
| [modulating functions of yeast rna]. | 1982 | 6293226 | |
| binding of clostridium perfringens enterotoxin to hepatocytes, small intestinal epithelial cells and vero cells. | clostridium perfringens enterotoxin binds specifically to hepatocytes, vero cells and rat intestinal epithelial cells, and causes membrane damage visible as "bubble" formation. fluorescein-labelled enterotoxin also binds specifically to the same cell types. 125i labelled enterotoxin shows one class of binding sites on both vero and rat intestinal epithelial cells. the number of binding sites decreases along the rat intestine, but the affinity constant is the same in different parts of the intest ... | 1982 | 6293246 |
| inhibition of protein synthesis by a tryptic polypeptide of clostridium perfringens type a enterotoxin. | the biological activity of clostridium perfringens enterotoxin can be tested more precisely and with a much higher sensitivity by using the inhibition of protein synthesis by vero cells, rather than the guinea pig skin test. tryptic peptides of the enterotoxin produced in the presence of different concentrations of sodium dodecyl sulfate (0-1%) have been tested for biological activity (vero cells) and inhibitory effect on cell-free protein synthesis (rabbit reticulocyte lysate). a fraction of tr ... | 1982 | 6293575 |
| characterization of bacteriocin 28 produced by clostridium perfringens. | bacteriocin 28, produced by clostridium perfringens, was characterized by gel filtration and sodium dodecyl sulfate - polyacrylamide gel electrophoresis as a glycoprotein with a molecule weight of approximately 100,000. density gradient centrifugation suggested a lower weight of 84,000. the bacteriocin bound firmly to phenyl-sepharose cl-4b gel, indicating hydrophobic properties, and elution from this gel with ethylene glycol clearly separated bacteriocin from the alpha and theta toxins of c. pe ... | 1982 | 6293695 |
| food borne infection with clostridium perfringens type a. | clostridium perfringens type a is one of the four most important bacterial agents causing food poisoning. differential biochemical characterization appears to be important because of certain confounding species. both the heat sensitive and resistant spore forming strains cause food poisoning. ubiquitous nature, ability to contaminate carcass intravitally, thermal resistance of spores, relatively short generation time at high incubation temperature, survival in chilled and frozen meat and ability ... | 1982 | 6293994 |
| pyruvoyl-dependent histidine decarboxylases from clostridium perfringens and lactobacillus buchneri. comparative structures and properties. | histidine decarboxylase (ec 4.1.1.22) was purified to homogeneity from clostridium perfringens and also from lactobacillus buchneri. both enzymes are composed of alpha and beta subunits, with an essential pyruvoyl group bound to the alpha subunit. in this respect and also in molecular weight of both the alpha and beta subunits and the native enzyme, they closely resemble the previously described (riley, w.d., and snell, e. e. (1970) biochemistry 9, 1485-1491) histidine decarboxylase from lactoba ... | 1983 | 6294108 |
| the potential of bacteriocin typing in the study of clostridium perfringens food poisoning. | a range of 49 bacteriocins was used to type 311 strains of clostridium perfringens isolated from food poisoning outbreaks. strains of same serotype within an outbreak showed similar patterns of susceptibility to bacteriocins, whereas strains of different serotype isolated from different sources produced many variations in bacteriocin susceptibility patterns. the 311 strains, along with isolates from a wide range of sources were screened for their ability to produce bacteriocins. a much greater p ... | 1982 | 6294146 |
| isolation and properties of metronidazole-resistant mutants of clostridium perfringens. | clostridium perfringens strains resistant to metronidazole and tinidazole were isolated from the sensitive parent strain cm288 after mutagenesis with n-methyl-n'-nitro-n-nitrosoguanidine. strain cm288 was already resistant to rifampicin and nalidixic acid; these genetic markers helped to confirm the identity of mutants. all mutants showed similar characteristics: they grew more slowly than the parent strain and failed to reach the same maximum turbidity; uptake of metronidazole and tinidazole fr ... | 1982 | 6294298 |
| [neonatal cecal perforation. clostridium perfringens enterocolitis with septicemia and hemolysis. total megacolon]. | 1982 | 6294584 | |
| [necrotic enteritis in piglets due to clostridium perfringens type "c"]. | 1982 | 6294823 | |
| [isolation of a purified theta-hemolysin from c1. perfingens type a and its use for titrating toxins and sera]. | purified cl. perfringens theta-hemolysin with a specific activity of 15-16 x 10(5) hu per mg of protein nitrogen, toxic for mice when injected intravenously, has been obtained by precipitation with zinc chloride, gel filtration through sephadex g-100 and ion-exchange chromatography on deae sephadex a-50. 1 dcl of the toxin is 0.2-0.4 mg. the yield of theta-hemolysin amounting to 20-25% has been achieved, the degree of its purification reaching 80- to 120-fold. equine anti-theta hemolytic serum h ... | 1982 | 6295033 |
| clostridium perfringens type a toxins in relation to nephritis and uric acid diathesis in captive willow ptarmigan (lagopus l. lagopus). | 1982 | 6295118 | |
| extraction of clostridium perfringens spores from bottom sediment samples. | two extraction-separation procedures were developed and evaluated for use in conjunction with the mcp membrane filter method for the enumeration of clostridium perfringens spores in bottom sediments. in the more facile of the two procedures, a distilled-water suspension of the sediment sample is pulse sonicated for 10 s and allowed to settle. portions of the supernatant are then removed for membrane filtration. this procedure is recommended for general use. the more complicated procedure is reco ... | 1982 | 6295278 |
| selective cytotoxicity of clostridium perfringens delta toxin on rabbit leukocytes. | clostridium perfringens delta toxin was selectively cytotoxic for various rabbit leukocyte populations. the sensitivity of these populations to the toxin varied, depending on the tissue from which they were derived, from 28% (appendix) to 41% (bone marrow) and 32% (spleen). macrophages were uniformly killed by delta toxin, whereas thymocytes were essentially resistant. selective cytotoxicity was correlated to the specific binding of the radiolabeled toxin by target cells. the relationship betwee ... | 1982 | 6295950 |
| in-vitro activity of ceftizoxime against anaerobic bacteria and comparison with other cephalosporins. | 1982 | 6296028 | |
| regulation of phosphatidylcholine biosynthesis in mammalian cells. i. effects of phospholipase c treatment on phosphatidylcholine metabolism in chinese hamster ovary cells and lm mouse fibroblasts. | addition of phospholipase c from clostridium perfringens to cultures of chinese hamster ovary (cho) cells resulted in rapid degradation of cellular phosphatidylcholine with concomitant release of phosphocholine. the rate of incorporation of radiolabeled choline into lipids was increased 2-fold in phospholipase c-treated cho cells as compared to untreated controls. the only enzyme in the pathway of phosphatidylcholine biosynthesis with increased activity in phospholipase c-treated cells was ctp:p ... | 1983 | 6296084 |
| regulation of phosphatidylcholine biosynthesis in mammalian cells. ii. effects of phospholipase c treatment on the activity and subcellular distribution of ctp:phosphocholine cytidylyltransferase in chinese hamster ovary and lm cell lines. | ctp:phosphocholine cytidylyltransferase was located in both the cytosolic and particulate fractions from chinese hamster ovary cells. the activity of the cytosolic form of the enzyme was greatly enhanced by incubation with sonicated preparations of several different lipids, although incubations with either phosphatidylcholine or 1,2-sn-diolein did not increase activity. the activation of the cytidylyltransferase in chinese hamster ovary cells treated with phospholipase c from clostridium perfrin ... | 1983 | 6296085 |
| clinical laboratory evaluation of a reverse camp test for presumptive identification of clostridium perfringens. | ninety-six percent of clostridium perfringens isolates from clinical specimens were reverse camp test positive, whereas several other clostridium species tested were reverse camp test negative. c. perfringens was detected by direct inoculation of clinical specimens to reverse camp plates, and the reverse camp procedure provided reliable presumptive identification of this organism. | 1982 | 6296196 |
| food poisoning due to clostridium perfringens in the united states. | 1983 | 6296240 | |
| spontaneous bacterial peritonitis and pneumoperitoneum. a false surgical emergency. | 1983 | 6296474 | |
| [location of exchangeable cations within the spore of clostridium perfringens]. | 1982 | 6296480 | |
| [interest in gnotoxenic systems for the study of host-microbial flora of the digestive tract]. | it is necessary to use experimental models in all studies of interactions between a host, its digestive tract microbial flora and the environment because these interactions are complex. the gnotoxenic animal, reared in an isolator as the axenic animal and harbouring a known microbial flora, constitutes either an analytic or mimetic experimental model. the gnotoxenic animal may be considered as an analytic model when used to determine which biotic or abiotic environmental factor of the host-anima ... | 1980 | 6296940 |
| [method of obtaining an erythrocyte diagnosticum for detecting cl. perfringens type a enterotoxin]. | erythrocytic immunoglobulin diagnostic reagent for detecting cl. perfringens enterotoxin in the indirect hemagglutination test has been obtained. the diagnostic reagent has been prepared on the basis of antibodies of cl. perfringens enterotoxin with the use of acroleinized tanned sheep red blood cells. the indirect hemagglutination test with this reagent is characterized by high specificity and sensitivity (the method permits to detect enterotoxin protein at a concentration of up to 0.005 microg ... | 1982 | 6297189 |
| [protective properties of theta-hemolysin obtained by affinity chromatography]. | the data on the study of the protective activity of theta hemolysin and cl. perfringens lecithinase preparations and the corresponding antitoxic sera obtained by indirect immune affinity chromatography are presented. experiments in mice and guinea pigs indicate that the injection of antihemolytic serum and immunization with anatheta hemolysin ensures the protection of the animals from theta toxin. the enrichment of analecithinase preparation with anatheta hemolysin has been found to increase its ... | 1982 | 6297191 |
| clostridium perfringens meningitis. | in this report, primary clostridium perfringens meningitis, a rare condition, is described in a 62-year-old man who developed fatal clostridial meningitis following surgical evacuation of a subdural hematoma. this is the third case report of clostridial meningitis as a complication of craniotomy. clostridial infection should be considered in any patient with meningitis with a history of surgical intervention, or head injury, even if only slight. | 1982 | 6298137 |
| clostridium perfringens and other anaerobes isolated from bile. | clostridium perfringens was isolated from bile in 13 cases of 150 patients examined. the serotypes of c perfringens strains isolated from bile and faeces were investigated using antisera to hobbs' type 1-17. two or more serological types were often found in a single specimen, but in the same patient the serotypes of c perfringens strains isolated from the bile were identical with those from the faeces. beta-glucuronidase production in these c perfringens serotypes was tested with the api-strep s ... | 1983 | 6298284 |
| sendai virus envelopes can mediate epstein-barr virus binding to and penetration into epstein-barr virus receptor-negative cells. | epstein-barr virus (ebv) receptor-negative cells were treated with uv-inactivated sendai virus (sv) or with reconstituted sv envelopes having a low hemolytic activity and then assayed for ebv binding or for susceptibility to ebv infection. ebv binding was assessed by using both unlabeled and fluoresceinated ebv preparations. it was found that sv or sv envelope treatment renders these cells able to bind ebv. various experiments were performed to clarify the mechanism of this sv-induced binding. t ... | 1983 | 6298467 |
| clostridial infection of a perinephric hematoma. | 1983 | 6298473 | |
| [emphysematous cholecystitis. microbiological study of a case]. | 1982 | 6298911 | |
| [antibacterial effects of niridazole. ii. effects on aerobic and anaerobic bacteria]. | niridazole which is chemically related to metronidazole is endowed with much better antibacterial activity. first, several genera of aerobic bacteria, such as salmonella and escherichia, are susceptible to niridazole, whereas metronidazole is completely ineffective. there exist, however, some particular strains which are naturally resistant to niridazole. the in vitro activity of niridazole is still increased, if these facultative anaerobic bacteria are tested under anaerobic growth conditions. ... | 1982 | 6299027 |
| neonatal diarrhea of pigs in quebec: infectious causes of significant outbreaks. | to evaluate the relative importance of the various enteropathogens causing neonatal diarrhea in quebec farrowing operations, observations were made on 749 diarrheic pigs from 325 outbreaks of diarrhea. they were one to 15 days of age, and were obtained alive for necropsy generally within 48 hours of the onset of diarrhea. some pigs were from severe, explosive outbreaks of diarrhea with high morbidity and mortality rates, while others were from herds with chronic neonatal diarrhea with lower morb ... | 1983 | 6299483 |
| enteritis necroticans (pigbel) an unrecognised preventable disease? | 1982 | 6299659 | |
| induction of ornithine decarboxylase by treatment of guinea pig lymphocytes with phospholipase c. | treatment of guinea pig lymphocytes with clostridium perfringens phospholipase c but not with naja naja snake venom phospholipase a2 increased ornithine decarboxylase activity. the increase in ornithine decarboxylase activity was suppressed by actinomycin d or cycloheximide, suggesting that de novo syntheses of rna and protein are necessary for the increase in the enzyme activity. these results suggest that the activation of phospholipase c rather than that of phospholipase a2 is responsible for ... | 1983 | 6299785 |
| [emphysematous cholecystitis caused by clostridium perfringens. sonographic and computer tomographic findings]. | 1983 | 6299914 | |
| application of serological typing to the investigation of outbreaks of clostridium perfringens food poisoning, 1970-1978. | serological typing was used as an epidemiological tool in the investigation of 524 outbreaks of clostridium perfringens food poisoning in the united kingdom and 37 outbreaks in other countries. five thousand five hundred and fifty-four (77%) of 7245 strains of c. perfringens associated with the 561 outbreaks were typable with the 75 food hygiene laboratory antisera; in 354 (63%) of these outbreaks a specific serotype was established as being responsible for the outbreak. an assessment is made of ... | 1980 | 6300225 |
| flow dichroism evidence for tilting of the bases when dna is in solution. | flow linear dichroism studies of dnas from clostridium perfringens, escherichia coli, and micrococcus luteus are extended into the vacuum uv region to 175 nm. the short-wavelength pi pi region shows a less negative reduced linear dichroism than the well-studied long-wavelength pi pi region. these results are independent of the type of dna and the concentration of salt over the range 0.001 to 1.0 m. this proves that the bases are not perpendicular to the flow orientation. assuming that the dna te ... | 1983 | 6300899 |
| mechanism of antimicrobial resistance and resistance transfer in anaerobic bacteria. | the antimicrobial susceptibility pattern of anaerobic bacteria has been changing over the past decade. this paper reviews the mechanisms by which these organisms have become resistant to selected antibiotics and reviews data demonstrating that bacteroides fragilis and clostridium perfringens possess systems for transferring resistance determinants. within bacteroides there is widespread resistance to penicillins, cephalosporins and tetracycline compounds while there have been reports of resistan ... | 1982 | 6300995 |
| [clostridium perfringens as a cause of food poisoning]. | 1982 | 6301099 | |
| immunisation schedule for the prevention of infectious necrotic enteritis caused by clostridium perfringens type c in piglets. | 1983 | 6301137 | |
| detection of clostridium perfringens type a enterotoxin by elisa. | 1982 | 6301213 | |
| configuration of substrate and products of n-acetylneuraminate pyruvate-lyase from clostridium perfringens. | to characterize the true substrate for aldolase from clostridium perfringens (optimum ph = 7.2) several experiments were carried out wherein the substrate neu5ac was generated in situ at ph 5.4 by the action of sialidase on its substrate neu5ac(alpha, 2 leads to 3) lactose. the alpha-anomer formed in this reaction was found to be split by aldolase at this ph into mannac and pyruvate. beta-neu5ac as such was not converted at ph 5.4. however, when it was first mutarotated until the equilibrium mix ... | 1983 | 6301475 |
| the specificity of viral and bacterial sialidases for alpha(2-3)- and alpha(2-6)-linked sialic acids in glycoproteins. | the anomeric specificity of six sialidases (vibrio cholerae, arthrobacter ureafaciens, clostridium perfringens, newcastle disease virus, fowl plague virus and influenza a2 virus sialidases) was assessed with sialylated antifreeze glycoprotein, ovine submandibular gland glycoprotein and alpha 1-acid glycoprotein, resialylated specifically in alpha(2-3) or alpha(2-6) linkage with n-acetylneuraminic acid or n-glycolylneuraminic acid using highly purified sialyltransferases. the rate of release of s ... | 1983 | 6301560 |
| vitamin e-enhanced humoral antibody response to clostridium perfringens type d in sheep. | 1983 | 6301619 | |
| susceptibility of 50 isolates of clostridium perfringens to cefotaxime, fosfomycin, penicillin g and vancomycin; variable tolerance for vancomycin. | 50 strains of clostridium perfringens recovered from human fecal specimens were examined for susceptibility to cefotaxime (ctx), fosfomycin (fosfo), penicillin g (pen g) and vancomycin (vanco) with the aid of a microtiter procedure. the geometric mean minimal inhibitory concentrations (mics) of ctx, fosfo, pen g and vanco were 0.81, 48.3, 0.15 and 0.33 micrograms/ml, respectively; the geometric mean bactericidal concentrations (mbcs) of the four drugs were 1.25, 109.8, 0.53 and 9.05 micrograms/m ... | 1983 | 6301777 |
| further development of a bacteriocin typing system for clostridium perfringens. | 1982 | 6302063 | |
| increase in osmotic fragility of bovine erythrocytes induced by bacterial phospholipases c. | bovine erythrocytes were treated with each of three bacterial phospholipases c; phosphatidylcholine-hydrolyzing phospholipase c (pcase) of clostridium perfringens, sphingomyelinase c (smase) of bacillus cereus and phosphatidylinositol-specific phospholipase c (piase) of bacillus thuringiensis. an increase in osmotic fragility was detected by means of a coil planet centrifugation (cpc) apparatus (biomedical systems co., tokyo) after the treatment with these enzymes. the peak of hemolysis normally ... | 1983 | 6302097 |
| studies on the interactions between phospholipids and membrane-bound enzymes in microsomes. effects of phospholipases c on kinetic properties of the glucose-6-phosphatase system in rat liver microsomes. | through kinetic analysis, the relationships between the glucose-6-phosphatase system and constituent phospholipids were studied in rat liver microsomes. when phosphoglycerides such as phosphatidylcholine and phosphatidylethanolamine on the microsomal membrane were hydrolyzed by phospholipase c of c. perfringens, the activities of glucose-6-p phosphohydrolase and glucose-6-p:glucose phosphotransferase both decreased with or without subsequent exposure to taurocholate. in these cases, the michaeli ... | 1983 | 6302099 |
| detection of clostridium perfringens enterotoxin by tissue culture and double-gel diffusion methods. | culture filtrates of four enterotoxin-producing strains of clostridium perfringens type a produced morphological changes in cells of 10 of 12 established mammalian cell lines tested, and particularly vero (african green-monkey kidney) and mdck (dog kidney) cells. double-gel diffusion and vero-cell tests detected enterotoxin in culture filtrates of, respectively, 29 (28%) and 56 (54%) of 103 strains of c. perfringens tested. the feasibility of cell-culture assays for the routine detection of c. p ... | 1983 | 6302258 |