Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| antigenic characteristics of leptotrichia buccalis (fusobacterium fusiforme). | gustafson, robert l. (university of illinois college of medicine, chicago) and arthur v. kroeger. antigenic characteristics of leptotrichia buccalis (fusobacterium fusiforme). j. bacteriol. 84:1313-1320. 1962.-thirty-nine isolates of an oral filamentous organism, usually termed leptotrichia buccalis trevisan (fusobacterium fusiforme), were isolated from materia alba or saliva. the cultural, morphological, and biochemical characteristics were similar to those reported by other investigators. aggl ... | 1962 | 13951402 |
| establishment of human indigenous bacteria in germ-free mice. | gibbons, r. j. (forsyth dental center and harvard school of dental medicine, boston, mass.), s. s. socransky, and b. kapsimalis. establishment of human indigenous bacteria in germ-free mice. j. bacteriol. 88:1316-1323. 1964.-thirteen strains of bacteria indigenous to the gingival crevice area of man were tested for their ability to establish as monocontaminants in germ-free mice. three facultative organisms, streptococcus mitis, staphylococcus albus, and a "diphtheroid," established, as well as ... | 1964 | 14234787 |
| intracellular production and extracellular release of oxygen radicals by pmns and oxidative stress on pmns during phagocytosis of periodontopathic bacteria. | in this study we investigated intracellular and extracellular oxygen radical production by polymorphonuclear leukocytes (pmns) during the phagocytosis of periodontopathic bacteria. in in vitro assays, bacteria of the species porphyromonas gingivalis, actinobacillus actinomycetemcomitans, and fusobacterium nucleatum were phagocytosed at 37 degrees c for 4 h by purified peripheral human pmns from healthy subjects (n = 6). superoxide production during phagocytosis was determined by flow cytometry a ... | 2003 | 14505184 |
| [ribotyping fusobacterium nucleatum isolates from healthy and diseased periodontium]. | fusobacterium nucleatum is one of the most frequently cultured bacteria from subgingival plaque. it can be isolated from active periodontal lesions as well as from healthy sites. currently, f. nucleatum has been divided into five subspecies. an early hypothesis suggested an association between certain subspecies and periodontal disease or health. on the other hand, a broad heterogeneity between f. nucleatum isolates has been suggested, so that the subspeciation scheme and therefore the proposed ... | 2003 | 14558378 |
| intracellular calcium in signaling human beta-defensin-2 expression in oral epithelial cells. | expression of human beta-defensins is correlated with differentiation in the oral epithelium, consistent with their function as part of the epithelial antimicrobial barrier. because calcium is a known regulator of epithelial differentiation, we tested the hypothesis that calcium concentration mediates beta-defensin expression. gingival epithelial cells were cultured in medium containing low calcium concentration (0.03 mm), then either changed to high extracellular calcium concentrations or stimu ... | 2003 | 14578498 |
| clinical presentation and bacteriologic analysis of infected human bites in patients presenting to emergency departments. | previous studies of infected human bites have been limited by small numbers of patients and suboptimal microbiologic methodology. we conducted a multicenter prospective study of 50 patients with infected human bites. seventy percent of the patients and assailants were young adult men. fifty-six percent of injuries were clenched-fist injuries and 44% were occlusional bites. most injuries were to the hands. fifty-four percent of patients were hospitalized. the median number of isolates per wound c ... | 2003 | 14614671 |
| fusobacterium nucleatum: a rare cause of bacteremia in neutropenic patients with leukemia and lymphoma. | although anaerobic bacteremias are uncommon in oncohematologic patients, nevertheless they have been considered an emergent problem in the last few years. fusobacterium nucleatum is an anaerobic gram-negative bacillus commonly present in the oral cavity and in the respiratory and genito-urinary tracts. over a 10-year period 18 episodes of f. nucleatum bacteremia in patients with hematological malignances (15 leukemias and 3 lymphomas) have been observed in our department of hematology. predispos ... | 2003 | 14616727 |
| expression of mhc class ii, cd70, cd80, cd86 and pro-inflammatory cytokines is differentially regulated in oral epithelial cells following bacterial challenge. | oral epithelium may play a regulatory role in local immune responses when interacting with bacteria. the present study was undertaken to investigate the effects of selected bacterial pathogens found in periodontal and endodontic infections on oral epithelial cells. expression of cell surface molecules (major histocompatibility complex (mhc) class ii, cd54, cd70, cd80 and cd86) and secretion of inflammatory cytokines (interleukin (il)-1beta, il-6, and tumor necrosis factor (tnf)-alpha) in respons ... | 2003 | 14622340 |
| the influence of organic load on the antimicrobial activity of different concentrations of naocl and chlorhexidine in vitro. | to evaluate bacterial growth after contact with sodium hypochlorite (naocl; 1 and 5%) and chlorhexidine (chx; 0.12, 0.5 and 1%) in vitro with or without the addition of organic material (bovine serum albumin (bsa) 0.5%). | 2003 | 14641423 |
| antagonistic interactions among fusobacterium nucleatum and prevotella intermedia with oral lactobacilli. | microbiota residing in oral ecosystems show great complexity because of the mutual interdependence of certain microorganisms that might influence their presence or absence at a given site. the aim of this work was to evaluate possible antagonistic interactions among fusobacterium nucleatum and prevotella intermedia, isolated from periodontal pockets, and lactobacillus casei, l. salivarius, l. rhamnosus and l. plantarum isolated from saliva. different tests were carried out using each strain as a ... | 2003 | 14643404 |
| culture-independent identification of pathogenic bacteria and polymicrobial infections in the genitourinary tract of renal transplant recipients. | renal transplant recipients are predisposed to urinary tract infections caused by both common uropathogens and opportunistic bacteria resulting frequently in significant polymicrobial infections. in this study, a culture-independent 16s rrna-based approach was established to identify unusual, fastidious, or anaerobic bacteria and to investigate bacterial diversity in urinary tract specimens. similarly sized amplicons encompassing the v6 to v8 region of the 16s rrna were analyzed with denaturing ... | 2003 | 14662931 |
| a comparison of the antibacterial efficacies of essential oils against oral pathogens. | cariogenic bacteria and periodontopathic bacteria are present in dental plaque as biofilms. in this study, we investigated the antibacterial effects of essential oils on the following oral bacteria: porphyromonas gingivalis, actinobacillus actinomycetemcomitans, fusobacterium nucleatum, streptococcus mutans, and streptococcus sobrinus. we tested manuka oil, tea tree oil, eucalyptus oil, lavandula oil, and romarinus oil and determined their minimum inhibitory concentration and minimum bactericida ... | 2004 | 14678476 |
| detection of subgingival calculus and dentine caries by laser fluorescence. | detection of subgingival calculus and dentine caries in the bottom of deep periodontal pockets is often difficult without visual observation. we thus examined the possibility of its detection using autofluorescence induced by laser irritation. | 2004 | 14687229 |
| innate immune response of oral and foreskin keratinocytes: utilization of different signaling pathways by various bacterial species. | the innate immune response is critical for the epithelial antimicrobial barrier. the human beta-defensins are small, cationic antimicrobial peptides that are made by epithelial cells and that play a role in mucosal and skin defenses. human beta-defensin 1 (hbd-1) is expressed constitutively in epithelial tissues, whereas hbd-2 and hbd-3 are expressed in response to bacterial stimuli or inflammation. previous studies showed that hbd-2 was induced by fusobacterium nucleatum cell wall extract witho ... | 2004 | 14688115 |
| multiplex fish analysis of a six-species bacterial biofilm. | established procedures use different and seemingly incompatible experimental protocols for fluorescent in situ hybridization (fish) with gram-negative and gram-positive bacteria. the aim of this study was to develop a procedure, based on fish and confocal laser scanning microscopy (clsm), for the analysis of the spatial organization of in vitro biofilms containing both gram-negative and gram-positive oral bacteria. biofilms composed of the six oral species actinomyces naeslundii, candida albican ... | 2004 | 14706749 |
| clonal similarity of salivary and nasopharyngeal fusobacterium nucleatum in infants with acute otitis media experience. | the environment of an infant's nasopharynx during acute otitis media (aom) favours the growth of anaerobic bacteria, which can be recovered frequently during infection, but hardly at all if the infant is healthy. the aim of this investigation was to identify the potential source and inoculation route of anaerobes that were present in the nasopharynx. eleven fusobacterium nucleatum isolates that were collected through the nasal cavity from the nasopharynx of eight infants with a history of aom, a ... | 2004 | 14729939 |
| effect of porphyromonas gingivalis vesicles on coaggregation of staphylococcus aureus to oral microorganisms. | vesicles from the outer membrane of porphyromonas gingivalis have the ability to aggregate a wide range of streptococcus spp., fusobacterium nucleatum, actinomyces naeslundii, and actinomyces viscosus. we found that in the presence of p. gingivalis vesicles, staphylococcus aureus coaggregated with streptococcus spp., and the mycelium-type candida albicans, but not the yeast type. autoaggregation of s. aureus in the presence of p. gingivalis vesicles is inhibited by l-arginine, l-lysine, and l-cy ... | 2003 | 14756532 |
| relationship between periodontal pocket sulfide levels and subgingival species. | many species implicated in the pathogenesis of periodontal disease produce volatile sulfur compounds (vsc). this investigation examined the relationship between levels of sulfide and subgingival bacterial species in the same periodontal pockets. | 2003 | 14761124 |
| gingival crevice microbiota from chinese patients with gingivitis or necrotizing ulcerative gingivitis. | the objective of this study was to quantitatively compare the bacterial population structure in plaque from the gingival margin of two groups of 21 chinese patients with gingivitis or necrotizing ulcerative gingivitis (nug). subjects were recruited in four dental clinics in eastern china. samples were quantitatively assessed by immunofluorescence and fluorescent in situ hybridization for taxa known to be associated with periodontal diseases. the analyses showed that the fusiform taxa (fusobacter ... | 2004 | 14871191 |
| microbiological examination of infected dental root canals. | the aim of this study was to investigate the root canal microbiota of primary and secondary root-infected canals and the association of constituent species with specific endodontic signs and symptoms. | 2004 | 14871344 |
| [acute abscess of the cerebrum indicative of bronchiectasis, caused by fusiformis fusiformis associated with corynebacterium parvum; cure by combined surgical and anti-infectious treatment]. | 1952 | 14935821 | |
| association of t cd4 lymphocyte levels and subgingival microbiota of chronic periodontitis in hiv-infected brazilians under haart. | the aim of this study was to determine the subgingival microbiota of hiv-infected patients with chronic periodontitis and different t cd4 lymphocyte levels under haart. | 2004 | 14970778 |
| glyceraldehyde-3-phosphate dehydrogenase of streptococcus oralis functions as a coadhesin for porphyromonas gingivalis major fimbriae. | cohesive interactions between porphyromonas gingivalis and plaque-forming bacteria, such as streptococcus oralis, are considered to play an important role in the colonization of p. gingivalis in periodontal sites. although p. gingivalis fimbriae have been reported to mediate coaggregation with s. oralis, the s. oralis molecule involved has not been identified. we identified the coadhesin of s. oralis atcc 9811 and purified it by affinity column chromatography. we found that the molecular mass of ... | 2004 | 14977937 |
| relationship between tongue coating and secretory-immunoglobulin a level in saliva obtained from patients complaining of oral malodor. | the aim of this study was to confirm the relationships between oral malodor and periodontal condition, oral malodor and tongue coating, and to investigate the secretory-immunoglobulin a (s-iga) level in saliva in relation to the accumulation of tongue coating. | 2003 | 15002886 |
| human beta-defensins 2 and 3 demonstrate strain-selective activity against oral microorganisms. | human beta-defensins 2 and 3 (hbd-2 and hbd-3) are inducible peptides present at sites of infection in the oral cavity. a few studies have reported broad-spectrum antimicrobial activity for both peptides. however, no comprehensive study has thoroughly investigated their potential against oral pathogens. the purpose of this study was to test the effectiveness of hbd-2 and hbd-3 against a collection of oral organisms (actinobacillus actinomycetemcomitans, fusobacterium nucleatum, porphyromonas gin ... | 2004 | 15004048 |
| fusobacterium nucleatum induces premature and term stillbirths in pregnant mice: implication of oral bacteria in preterm birth. | fusobacterium nucleatum is a gram-negative anaerobe ubiquitous to the oral cavity. it is associated with periodontal disease. it is also associated with preterm birth and has been isolated from the amniotic fluid, placenta, and chorioamnionic membranes of women delivering prematurely. periodontal disease is a newly recognized risk factor for preterm birth. this study examined the possible mechanism underlying the link between these two diseases. f. nucleatum strains isolated from amniotic fluids ... | 2004 | 15039352 |
| geographical differences in bacteria detected in endodontic infections using polymerase chain reaction. | the polymerase chain reaction (pcr) is an innovative nucleic acid-based assay that has the highest sensitivity of any microbiological technique for the detection of bacteria. the purpose of this study was to use pcr to detect the presence of specific species of bacteria in samples collected from two geographical locations. microbial samples from abscesses of endodontic origin were collected from patients in portland, oregon, and rio de janeiro, brazil. pcrs with species-specific oligonucleotide ... | 2004 | 15055430 |
| [dynamic observation of periodontal pathogens and cariogenic bacteria in modified chemostat using confocal laser scanning microscopy]. | to study dynamic relation between periodontal pathogens and cariogenic bacteria under analogous oral environment. | 2004 | 15061891 |
| blindness from septic thrombophlebitis of the orbit and cavernous sinus caused by fusobacterium nucleatum. | 2004 | 15078687 | |
| risk factors for periodontitis in hiv patients. | the purpose of this study was to identify risk factors for periodontitis associated with human immunodeficiency virus (hiv) infection. | 2004 | 15102043 |
| identification of a fusobacterium nucleatum 65 kda serine protease. | a 65 kda protease was partially purified from extracellular vesicles of fusobacterium nucleatum cultures by preparative sds-page followed by electroelution. the ph optimum of the protease is 7.5-8.0 and its activity could be inhibited by serine protease inhibitors. the protease was found to degrade the extracellular matrix proteins fibrinogen and fibronectin as well as collagen i and collagen iv which were degraded at 37 degrees c but not at 28 degrees c, indicating the presence of a gelatinase ... | 2004 | 15107066 |
| induction of calprotectin release by porphyromonas gingivalis lipopolysaccharide in human neutrophils. | calprotectin, a major cytosolic protein of leukocytes, is detected in neutrophils, monocytes/macrophages, and epithelial cells. this protein is known to be a marker for several inflammatory diseases and is detected in inflammatory gingival tissue with periodontal disease. recently, we found that the calprotectin level in gingival crevicular fluid from periodontitis patients was significantly higher than that of healthy subjects. however, the regulation of calprotectin in periodontal disease is u ... | 2004 | 15107070 |
| selection for high-level resistance by chronic triclosan exposure is not universal. | to investigate the effect of triclosan exposure on the antimicrobial susceptibilities of numerically important dental bacteria. | 2004 | 15117935 |
| microbiologic diagnostics at titanium implants. | the microbiota found at periimplant lesions have been shown to contain putative periodontal pathogens as well as opportunistic species such as staphylococcus spp, enterics, and candida spp. therefore, a microbiologic diagnosis may be of value as guidance before treatment of such lesions. | 2003 | 15127993 |
| the role of lipopolysaccharide in infectious bone resorption of periapical lesion. | the role of lipopolysaccharide (lps) in periapical lesion-induced bone resorption was investigated. polymyxin b (pmb), a specific inhibitor of lps, was evaluated to treat the apical lesion. | 2004 | 15128058 |
| surveillance of susceptibility patterns in 1297 european and us anaerobic and capnophilic isolates to co-amoxiclav and five other antimicrobial agents. | in vitro susceptibility data were collected for co-amoxiclav and other antimicrobial agents against 1297 recent anaerobe isolates collected in europe and the usa. the co-amoxiclav (amoxicillin/clavulanic acid) mic(50/90)s (amoxicillin/clavulanic acid concentration in a ratio of 2:1, expressed in terms of amoxicillin concentration in mg/l) were 0.5/4 for bacteroides fragilis, </=0.125/1 for prevotella species, </=0.125/0.25 for fusobacterium nucleatum, 0.5/1 for eikenella corrodens, 0.25/8 for pe ... | 2004 | 15128729 |
| another good reason to floss. | 2004 | 15141231 | |
| clonal persistence of oral fusobacterium nucleatum in infancy. | once established, early-colonizing bacterial species tend to persist in the mouth. to obtain detailed information on the population dynamics of early-colonizing oral anaerobes, we examined the clonal diversity and persistence of clones among oral fusobacterium nucleatum populations during the first 2 yrs of life. consecutive salivary samples from 12 infants, collected at 2, 6, 12, 18, and 24 mos of age, yielded a total of 546 f. nucleatum isolates for clonal typing with arbitrarily primed pcr (a ... | 2004 | 15153460 |
| apical periodontitis development and bacterial response to endodontic treatment. experimental root canal infections in monkeys with selected bacterial strains. | in six monkeys, 160 root canals were inoculated with a combination of four bacterial strains belonging to species streptococcus milleri, peptostreptococcus anaerobius, prevotella oralis, and fusobacterium nucleatum. in two other monkeys, 24 root canals were inoculated with a five-strain combination consisting of these strains and a strain of enterococcus faecalis. all strains were previously isolated from an infected monkey root canal. after 8-12 months, survival of the strains was recorded bact ... | 2004 | 15154917 |
| perforation repair comparing two types of mineral trioxide aggregate. | the purpose of this study was to evaluate the ability of two types of mineral trioxide aggregate (mta) to seal furcal perforations in extracted human molars using an anaerobic bacterial leakage model. forty, human, maxillary and mandibular molars were randomly divided into two experimental groups of 18, with two teeth used as positive controls and two teeth without perforations used as negative controls. experimental group 1 was repaired with gray-colored mta and group 2 with off-white-colored m ... | 2004 | 15167471 |
| the effects of chlorhexidine gluconate (0.12%) on the antimicrobial properties of tooth-colored proroot mineral trioxide aggregate. | the purpose of this in vitro study was to determine whether the substitution of 0.12% chlorhexidine gluconate for sterile water as a mixing agent would enhance the antimicrobial activity of tooth-colored proroot mineral trioxide aggregate (mta) against actinomyces odontolyticus (atcc17982), fusobacterium nucleatum (atcc2586), streptococcus sanguis (atcc10556), enterococcus faecalis (er3/2s), escherichia coli (sm10lambdapir), staphylococcus aureus (atcc6538), pseudomonas aeruginosa (ume), and can ... | 2004 | 15167473 |
| modulation of the antibody response by porphyromonas gingivalis and fusobacterium nucleatum in a mouse model. | successive immunization of mice with fusobacterium nucleatum and porphyromonas gingivalis has been shown to modulate the specific serum igg responses to these organisms. the aim of this study was to investigate these antibody responses further by examining the igg subclasses induced as well as the opsonizing properties of the specific antibodies. serum samples from balb/c mice immunized with f. nucleatum (gp1-f), p. gingivalis (gp2-p), p. gingivalis followed by f. nucleatum (gp3-pf) f. nucleatum ... | 2004 | 15209995 |
| effect of supragingival plaque control on subgingival microflora and gingivo-periodontal tissues. | the aim of this study was to carry out a short term evaluation of the effect of supragingival plaque control on the subgingival microflora and gingivoperiodontal tissues in patients with moderate and severe periodontitis. a total of 92 sites from 23 patients were studied; patients ranged between 35 and 60 years of age (x 46.4 +/- 1.71). plaque index (pi), gingival index (gi), bleeding on probing (bp), probing depth (pd), and attachment level (al) were measured. subgingival microflora of the same ... | 2000 | 15211927 |
| subgingival microbiota of chilean patients with chronic periodontitis. | an association between race/ethnicity and the composition of the subgingival microbiota has been found in chronic periodontitis. a study was undertaken to determine the characteristics of the subgingival microbiota of chronic periodontitis in chileans residing in santiago. | 2004 | 15212354 |
| comparison of systemic flurithromycin therapy and clinical procedures in the treatment of periodontal diseases. | the purpose of the present investigation was to evaluate, in 20 periodontal patients, the microbial and clinical effects of flurithromycin therapy plus scaling and root planning (srp) in comparison with srp alone. clinical assessments of plaque, bleeding on probing and pocket depth were made prior to srp alone and srp plus flurithromycin therapy (375 mg twice daily for 5 days) and after both treatments. subgingival plaque samples (n. 180) were taken prior to and after both treatments and analyze ... | 2004 | 15216949 |
| evaluation of the whole genome dna-dna hybridization technique to identify bacteria in histological sections of periradicular lesions. | whole genome dna-dna hybridization has been used to identify bacteria in periradicular lesions partly because there is no amplification of the bacteria, therefore, minor contaminants are not detected. there are, however, potential pitfalls with this technique, including inability to distinguish dead bacteria, cross-reactions of species within a genus, and inability to detect species present in low numbers because of loss of dna during extraction and purification. alternatively, inadequate extrac ... | 2004 | 15220650 |
| portal vein thrombosis associated with fusobacterium nucleatum septicemia in a patient with ulcerative colitis. | 2004 | 15232367 | |
| development of a multispecies oral bacterial community in a saliva-conditioned flow cell. | microbial communities within the human oral cavity are dynamic associations of more than 500 bacterial species that form biofilms on the soft and hard tissues of the mouth. understanding the development and spatial organization of oral biofilms has been facilitated by the use of in vitro models. we used a saliva-conditioned flow cell, with saliva as the sole nutritional source, as a model to examine the development of multispecies biofilm communities from an inoculum containing the coaggregation ... | 2004 | 15240317 |
| active domains of salivary statherin on apatitic surfaces for binding to fusobacterium nucleatum cells. | fusobacterium nucleatum can bind to saliva-coated tooth surfaces. however, the nature of the domains of salivary protein that interact with f. nucleatum remains unclear. the ability of individual proteins in human submandibular-sublingual saliva (hsmsl) to bind f. nucleatum cells was examined by dot blot assay; statherin displayed the strongest binding activity. statherin binding sites were determined based on binding of (125)i-labelled f. nucleatum to statherin-coated hydroxyapatite (shap) bead ... | 2004 | 15256578 |
| [purification and characterization of sanguicin--a bacteriocin produced by streptococcus sanguis]. | streptococcus sanguis plays an important role in maintaining the periodontal microecological balance. previous studies have demonstrated that sanguicin--a kind of bacteriocin produced by s. sanguis has a prominent function of inhibiting the growth of putative periodontopathic bacteria (ppb). the aim of this study was to purify sanguicin and investigate its characters. | 2004 | 15291127 |
| implant therapy following liver transplantation: clinical and microbiological results after 10 years. | the number of immunocompromised patients is increasing as a consequence of successful organ transplantation. placing dental implants in these patients has been questioned because of their increased risk for infections. the 10-year follow-up data of a 71-year-old liver transplant recipient with long-term immunosuppressive therapy is reported. six months after liver transplantation, two interforaminal implants were inserted in the edentulous mandible, and an overdenture using non-rigid telescopic ... | 2004 | 15295960 |
| identification and sequencing of bacterial rdnas in culture-negative amniotic fluid from women in premature labor. | our objective was to identify bacterial species present in culture-negative but 16s rdna-positive amniotic fluid samples from women in preterm labor. amniotic fluid from 69 women in preterm labor was cultured and examined for the proinflammatory cytokine interleukin-6 (il-6). polymerase chain reaction technology was used to detect highly conserved bacterial ribosomal dna sequences (16s rdnas). as previously reported, 16s rdnas were identified in 15 (94%) of 16 culture-positive amniotic fluid sam ... | 2004 | 15311367 |
| the participation of nitric oxide in peritoneal exudate cell cytotoxicity of mice by fusobacterium nucleatum. | previously we reported that mice infected recurrently with live fusobacterium nucleatum(fn) synthesize a significant amount of no between 12 hr and 24 hr after fn injection. fn is a gram-negative rod periodontal pathogen. no could not be induced by heat-killed fn or in untreated mice. this no, derived from the inos after infection of live fn, was not involved in the fn reduction because fn clearance occurs within 6 hr. we investigated in this study whether this no was involved in cytotoxicity in ... | 2004 | 15322341 |
| beta-lactamase production and antimicrobial susceptibility of subgingival bacteria from refractory periodontitis. | this study assessed the extent of beta-lactamase-producing bacteria in subgingival plaque samples obtained from 25 patients with refractory marginal periodontitis in the usa. beta-lactamase-positive isolates were characterized using commercial diagnostic kits and partial sequencing of the 16s rrna gene. the susceptibilities to different antimicrobial agents were tested and, in addition, the isolates were screened for the presence of extended spectrum beta-lactamases (esbls). beta-lactamase-produ ... | 2004 | 15327642 |
| selected endodontic pathogens in the apical third of infected root canals: a molecular investigation. | bacteria located at the apical portion of the root canals are conceivably in a strategic position to induce damage to the periradicular tissues and resulting inflammatory diseases. this study sought to investigate the prevalence of 11 selected putative endodontic pathogens in the apical third of infected root canals associated with periradicular lesions. the apical root portion of 23 extracted teeth with carious pulpal exposures and attached periradicular lesions was sectioned, and the root cana ... | 2004 | 15329567 |
| clinical and microbiological changes associated with the use of combined antimicrobial therapies to treat "refractory" periodontitis. | the present investigation examined clinical and microbial changes after a combined aggressive antimicrobial therapy in subjects identified as "refractory" to conventional periodontal therapy. | 2004 | 15367191 |
| fusobacterium canifelinum sp. nov., from the oral cavity of cats and dogs. | fourteen strains of gram-negative, anaerobic, fluoroquinolone-resistant, non-sporulating rods were isolated from various infections in cats and dogs, as well as from wounds in humans after cat- or dog-bites. these strains were characterized by sequencing of the 16s-23s rdna internal transcribed spacer (its) regions, 16s rdna, dna-dna hybridization, phylogenetic analysis, and phenotypic tests. the results indicate that the novel strains belong to a distinct species, closely related to fusobacteri ... | 2004 | 15368845 |
| mesenteric adenitis and portal vein thrombosis due to fusobacterium nucleatum. | we report the first description of portal and mesenteric vein thrombosis associated with suppurative mesenteric adenitis in a 71-year-old woman. the bacterium detected in mesenteric lymph nodes was fusobacterium nucleatum, an anaerobic gram-negative bacillus. our patient had a clinical syndrome of pharyngitis and fever preceding portal vein thrombosis. abdominal symptoms improved with antibiotics and anticoagulant therapy. this location of f. nucleatum in mesenteric lymph nodes provides an inter ... | 2004 | 15371934 |
| dehydration of (r)-2-hydroxyacyl-coa to enoyl-coa in the fermentation of alpha-amino acids by anaerobic bacteria. | several clostridia and fusobacteria ferment alpha-amino acids via (r)-2-hydroxyacyl-coa, which is dehydrated to enoyl-coa by syn-elimination. this reaction is of great mechanistic interest, since the beta-hydrogen, to be eliminated as proton, is not activated (pk 40-50). a mechanism has been proposed, in which one high-energy electron acts as cofactor and transiently reduces the electrophilic thiol ester carbonyl to a nucleophilic ketyl radical anion. the 2-hydroxyacyl-coa dehydratases are two-c ... | 2004 | 15374661 |
| in situ biofilm formation by multi-species oral bacteria under flowing and anaerobic conditions. | an understanding of biofilm behavior of periodontopathic bacteria is key to the development of effective oral therapies. we hypothesized that interspecies bacterial aggregates play an important role in anaerobic biofilm establishment and proliferation, and contribute to the survivability of the biofilm against therapeutic agents. the system developed in this study assessed a multi-species (streptococcus gordonii, actinobacillus actinomycetemcomitans, and fusobacterium nucleatum) biofilm formatio ... | 2004 | 15381723 |
| binding of pro-matrix metalloproteinase 9 by fusobacterium nucleatum subsp. nucleatum as a mechanism to promote the invasion of a reconstituted basement membrane. | in this study, we investigated the ability of fusobacterium nucleatum subsp. nucleatum to increase its tissue-invasive potential by acquiring cell-associated human matrix metalloproteinase 9 (mmp-9) activity. binding of pro-mmp-9 to fusobacteria was demonstrated by enzyme-linked immunosorbent assay. zymography and a colorimetric assay showed that bound pro-mmp-9 can be converted into a proteolytically active form. the potential contribution of this acquired host activity in tissue invasion was d ... | 2004 | 15385524 |
| cavernous sinus thrombosis and cerebral infarction caused by fusobacterium nucleatum infection. | we report an unusual case of fusobacterial infection with secondary intracranial invasion. the condition was complicated by a cavernous sinus thrombosis and ischemic stroke. the patient was a 63-year-old woman with no history of systemic disease who had undergone a tooth extraction before the onset of symptoms. she initially suffered from sphenomaxillary sinusitis and a cavernous sinus thrombosis, and subsequently developed meningitis. cerebrospinal fluid examination suggested a pyogenic infecti ... | 2004 | 15455548 |
| periodontal pathogens in atheromatous plaques. a controlled clinical and laboratory trial. | a possible relationship between periodontitis and cardiovascular disease has been suggested. the aims of this controlled clinical study were: (i) to ascertain the presence of periodontal bacteria dna [actinobacillus actinomycetemcomitans, fusobacterium nucleatum, porphyromonas gingivalis, prevotella intermedia, tannerella forsythensis (formerly bacteroides forsythus)] in carotid atheromatous plaques and (ii) to assess the concomitant presence of the same periodontal bacteria dna, if any, in peri ... | 2004 | 15491349 |
| alkali-resistant bacteria in root canal systems. | the aim of this study was to isolate and identify alkali-resistant bacteria from the dentin of infected root canals. bacteria from homogenized dentin powder made up from infected root canal walls from human teeth were cultured on buffer-enriched brain heart infusion agar supplemented with 4% sheep blood (bhi-blood agar), adjusted to ph 7.0, 9.0 or 10.0. incubation took place for 7 days at 37 degrees c in an anaerobic glove box. bacterial strains selected according to colony and morphology were s ... | 2004 | 15491465 |
| microbiological evaluation of endodontic files after cleaning and steam sterilization procedures. | infection control procedures are essential for modern dental practice and they are continually evolving to meet the dental profession's high standards. the present study evaluated the efficacy of two cleaning procedures to reduce bacterial numbers on endodontic files, and evaluated the effect of biological debris on the subsequent sterilization of files. | 2004 | 15497355 |
| antiproliferative activity of actinobacillus (haemophilus) actinomycetemcomitans and fusobacterium nucleatum in peripheral blood mononuclear cells. | several studies indicate actinobacillus (haemophilus) actinomycetemcomitans and fusobacterium nucleatum as etiologic agents of periodontal disease. immunosuppressive factors produced by microorganisms probably contribute to the initiation and evolution of this disease. this study evaluated the antiproliferative activity of ammonium precipitate fractions of a. (h.) actinomycetemcomitans and f. nucleatum isolates from humans and marmosets both with and without periodontal disease. all a. (h.) acti ... | 2004 | 15501650 |
| rate of cultivable subgingival periodontopathogenic bacteria in chronic periodontitis. | although microbiological studies have identified more than 400 bacterial species in periodontal pockets, only a limited number have been implicated as periodontal pathogens. the purpose of this study was to investigate the incidence of cultivable subgingival periodontopathogenic bacteria in chronic periodontitis. bacterial samples were collected with sterile paper points from the deepest periodontal pockets ((5 mm) of 203 patients: 92 males and 111 females, aged 35-55 years. the samples were cul ... | 2004 | 15508748 |
| phylogenetic analysis of fusobacterium necrophorum, fusobacterium varium and fusobacterium nucleatum based on gyrb gene sequences. | the nucleotide sequences of the dna gyrase b subunit gene (gyrb) of fusobacterium necrophorum subsp. necrophorum, f. necrophorum subsp. funduliforme and f. varium were determined and analyzed together with those of f. nucleatum subsp. nucleatum and f. nucleatum subsp. vincentii. on the phylogenetic tree constructed, the strains of each fusobacterial species formed distinct clusters with deep sublines. the degree of sequence similarity within each cluster was 93.2% or more, whereas similarities b ... | 2004 | 15528856 |
| investigation of the effect of the coronal restoration quality on the composition of the root canal microflora in teeth with apical periodontitis by means of t-rflp analysis. | to investigate the effect of the radiographic and clinical quality of coronal restorations on the composition of the root canal flora of teeth with necrotic pulps and teeth with root fillings associated with apical periodontitis. | 2004 | 15548272 |
| [an unusual case of hepatic abscess]. | right upper quadrant pain with chronic malaise and fever can be the clinical manifestation of a hepatic infection. an uncommon cause for the disease is hepatic actinomycosis. actinomycosis was common in the preantibiotic era but is less frequent nowadays; consequently its timely recognition has become more difficult. the clinical and radiological findings often resemble other inflammatory and neoplastic lesions. we report a case of a mixed anaerobic liver abscess including fusobacteria and actin ... | 2004 | 15553899 |
| ["in vitro" activity of ten antimicrobial agents against anaerobic bacteria. a collaborative study, 1999-2002]. | the antimicrobial activity of ampicillin, ampicillin-sulbactam, cefoxitin, ceftriaxone, imipenem, piperacillin, piperacillin-tazobactam, clindamycin, metronidazole, and azitromycin was assesed against 166 strains of anaerobic bacteria recovered from eight hospitals in buenos aires. the strains studied were bacteroides fragilis group (65), fusobacterium spp. (26), prevotella spp. (21), porphyromonas spp. (10), clostridium difficile (10), other clostridia (12), and gram-positive cocci (22). the mi ... | 2004 | 15559195 |
| single species biofilm-forming ability of root canal isolates on gutta-percha points. | the participation of bacterial biofilms in the over-filled gutta-percha points associated with refractory periapical periodontitis has recently been reported. this study investigated the initial biofilm-forming ability of root canal isolates (enterococcus faecalis, streptococcus sanguis, strep. intermedius, strep. pyogenes, staphylococcus aureus, fusobacterium nucleatum, propionibacterium acnes, porphyromonas gingivalis and prevotella intermedia) on gutta-percha points in vitro. each bacterial s ... | 2004 | 15560836 |
| killing of periodontopathogenic bacteria by photodynamic therapy. | the aim of this study was to evaluate a new approach for killing periodontopathogenic bacteria using photodynamic therapy (pdt). | 2004 | 15562911 |
| evolutionary relationships of fusobacterium nucleatum based on phylogenetic analysis and comparative genomics. | the phylogenetic position and evolutionary relationships of fusobacteria remain uncertain. especially intriguing is their relatedness to low g+c gram positive bacteria (firmicutes) by ribosomal molecular phylogenies, but their possession of a typical gram negative outer membrane. taking advantage of the recent completion of the fusobacterium nucleatum genome sequence we have examined the evolutionary relationships of fusobacterium genes by phylogenetic analysis and comparative genomics tools. | 2004 | 15566569 |
| characterization of the novel fusobacterium nucleatum plasmid pkh9 and evidence of an addiction system. | fusobacterium nucleatum is an important oral anaerobic pathogen involved in periodontal and systemic infections. studies of the molecular mechanisms involved in fusobacterial virulence and adhesion have been limited by lack of systems for efficient genetic manipulation. plasmids were isolated from eight strains of f. nucleatum. the smallest plasmid, pkh9 (4,975 bp), was characterized and used to create new vectors for fusobacterial genetic manipulation. dna sequence analysis of pkh9 revealed an ... | 2004 | 15574887 |
| periimplantitis. | orodental rehabilitation through the use of implants offers very high success rates. in this paper, we describe some of the complications involved with this technique, such as periimplant disease and, within this category, periimplantitis, an inflammatory reaction in which there is a loss of the bony support of the implant accompanied by inflammation. the aetiology of the disease is conditioned by the status of the tissue surrounding the implant, implant design, degree of roughness, the poor ali ... | 2004 | 15580138 |
| the aetiology and treatment of oral halitosis: an update. | halitosis refers to the condition of offensive mouth odour. more than 90% of cases of halitosis originate from the oral cavity. the implicated bacteria (fusobacterium nucleatum, prevotella intermedia, and tannerella forsythensis) are located in stagnant areas in the oral cavity, such as the dorsal surface of tongue, periodontal pockets, and interproximal areas. these bacteria proteolyse the amino acids releasing volatile sulphur compounds. the management of halitosis involves determining and eli ... | 2004 | 15591601 |
| coaggregation profiles of the microflora from root surface caries lesions. | bacterial coaggregation reactions between different species and the auto aggregation of the same species are associated with the initiation and development of dental plaque and biofilms. as no such data is available on isolates from root caries lesions, we evaluated, by a visual, semi-quantitative scoring system and a spectrophotometric, quantitative assay, the coaggregation of 22 different wild-type microbial species comprising ten bacterial genera and a single candida spp. the quantitative coa ... | 2005 | 15598414 |
| synergy between tannerella forsythia and fusobacterium nucleatum in biofilm formation. | during dental plaque formation, the interaction of different organisms is important in the development of complex communities. fusobacterium nucleatum is considered a 'bridge-organism' that facilitates colonization of other bacteria by coaggregation-mediated mechanisms and possibly by making the environment conducive for oxygen intolerant anaerobes. these studies were carried out to determine whether coaggregation between f. nucleatum and tannerella forsythia is important in the formation of mix ... | 2005 | 15612944 |
| inhibitory effects of various micro-organisms on the growth of helicobacter pylori. | to examine the in vitro influence of various bacteria species on helicobacter pylori (hp) growth. | 2005 | 15613007 |
| differential regulation of cytokine genes in gingival epithelial cells challenged by fusobacterium nucleatum and porphyromonas gingivalis. | il-8 mrna in human gingival epithelial cells (hgecs) is up-regulated by fusobacterium nucleatum, and up-/down-regulated by porphyromonas gingivalis in a complex interaction in the early stages (< or = 4 h) after infection. the mechanisms involved in this regulation in response to f. nucleatum and/or p. gingivalis infection, and identification of co-regulated cytokine genes, are the focus of this investigation. heat, formalin or protease treatment of f. nucleatum cells attenuated the il-8 mrna up ... | 2004 | 15619426 |
| attachment of oral gram-negative anaerobic rods to a smooth titanium surface: an electron microscopy study. | attachment of bacteria to titanium may differ not only between bacterial species but also between strains within a species. the aim of the present in vitro study was to examine differences in bacterial attachment using 4 gram-negative anaerobic species of bacteria that are considered potential periodontal pathogens. | 2004 | 15623054 |
| phototoxic effect of visible light on porphyromonas gingivalis and fusobacterium nucleatum: an in vitro study. | the antibacterial effect of visible light irradiation combined with photosensitizers has been reported. the objective of this was to test the effect of visible light irradiation without photosensitizers on the viability of oral microorganisms. strains of porphyromonas gingivalis, fusobacterium nucleatum, streptococcus mutans and streptococcus faecalis in suspension or grown on agar were exposed to visible light at wavelengths of 400-500 nm. these wavelengths are used to photopolymerize composite ... | 2004 | 15623322 |
| the role of anaerobic bacteria in tonsillitis. | this review summarizes the information that supports the potential importance of anaerobic bacteria in tonsillitis. some anaerobic bacteria possess interfering capability with group a beta-hemolytic streptococci (gabhs) and other pathogens. the possible role of anaerobes in the acute inflammatory process in the tonsils is supported by several observations: anaerobes have been isolated from the cores of tonsils of patients with recurrent gabhs and non-gabhs tonsillitis (nst); the recovery of anae ... | 2005 | 15627441 |
| effectiveness of a triclosan/copolymer dentifrice on microbiological and inflammatory parameters. | according to the us surgeon general's report, "oral health in america," published in 2000, most adults in the united states show some degree of periodontal pathology, with severe periodontal diseases affecting about 14% of middle-aged adults. periodontal diseases are polymicrobial-induced inflammatory diseases, and they vary from mild gingival inflammation to severe deterioration of the periodontium, ie, loss of periodontal supportive tissues and, ultimately, tooth loss. new evidence shows that ... | 2004 | 15645886 |
| fusobacterium nucleatum increases collagenase 3 production and migration of epithelial cells. | fusobacterium nucleatum is closely associated with human periodontal diseases and may also be a causative agent in other infections, such as pericarditis, septic arthritis, and abscesses of tonsils and liver. initiation and outcome of infective diseases depend critically on the host cell signaling system altered by the microbe. production of proteinases by infected cells is an important factor in pericellular tissue destruction and cell migration. we studied binding of f. nucleatum to human epit ... | 2005 | 15664960 |
| periodontal infections and pre-term low birth weight: a case-control study. | pre-term delivery of low-birth-weight infants [pre-term low birth weight (plbw)] remains a significant public health issue and a major cause of neonatal death and long-term health problems. there is a growing consensus that infections remote from fetal-placental unit may influence plbw infants. recent studies have suggested that maternal periodontal disease may be an independent risk factor for plbw. the purpose of the present study was to evaluate the possible link between periodontal infection ... | 2005 | 15691348 |
| dna-dna hybridization demonstrates multiple bacteria in osteoradionecrosis. | bone necrosis secondary to radiation was previously attributed to trauma of devitalized bone and microbiological sepsis. however, conventional microbiological technique has failed to demonstrate microorganisms throughout osteoradionecrotic bone, claimed to be hypoxic, hypovascular and hypocellular. the aim of the present study was to examine such bone for bacteria using dna-dna hybridization. compared to standard culture methods this technique enables the investigation of a vast number of bacter ... | 2005 | 15695050 |
| molecular analysis of microbial diversity in advanced caries. | real-time pcr analysis of the total bacterial load in advanced carious lesions has shown that the total load exceeds the number of cultivable bacteria. this suggests that an unresolved complexity exists in bacteria associated with advanced caries. in this report, the profile of the microflora of carious dentine was explored by using dna extracted from 10 lesions selected on the basis of comparable total microbial load and on the relative abundance of prevotella spp. using universal primers for t ... | 2005 | 15695690 |
| development and characterization of a simple perfused oral microcosm. | to validate perfused, inline, filter-based fermentation systems (multiple sorbarod devices, msd) for their ability to maintain stable oral bacterial communities. msd enable replicate (n=5) microcosm biofilms (bf) to be established and sampled, together with their perfusates (pa, cells in eluted medium). | 2005 | 15715865 |
| antimicrobial actions of benzimidazoles against oral streptococci. | benzimidazoles, such as lansoprazole and omeprazole, are used extensively as proton-pump inhibitors to control stomach acid secretion and also have antimicrobial actions against helicobacter pylori. our objective was to determine whether they are potentially useful antimicrobials against oral bacteria. | 2005 | 15720569 |
| impact of sample storage on detection of periodontal bacteria. | information on the impact of sample storage prior to analysis by dna methods is limited. the aim of this study was to investigate the effect of subgingival sample storage on bacterial detection and enumeration. | 2005 | 15720575 |
| thiazolidinedione (pioglitazone) blocks p. gingivalis- and f. nucleatum, but not e. coli, lipopolysaccharide (lps)-induced interleukin-6 (il-6) production in adipocytes. | an elevated level of c-reactive protein (crp) predicts the future development of coronary heart disease. periodontitis appears to up-regulate crp. crp is produced by hepatocytes in response to interleukin-6 (il-6). a major source of il-6 in obese subjects is adipocytes. we hypothesized that lipopolysaccharide (lps) from periodontal pathogens stimulated adipocytes to produce il-6, and that the production was suppressed by the drugs targeted against insulin resistance, thiazolidinedione (pioglitaz ... | 2005 | 15723863 |
| quantitative detection of volatile sulfur compound- producing microorganisms in oral specimens using real-time pcr. | it is well-known that some periodontopathic bacteria, especially porphyromonas gingivalis, fusobacterium nucleatum, tannerella forsythia (formerly bacteroides forsythus or tan. forsythensis), and treponema denticola, actively produce volatile sulfur compounds (vscs), such as h2s and ch3sh. we previously reported a qualitative relationship between periodontopathic bacteria and vscs; however, a quantitative analysis of periodontopathic bacteria in oral specimens is required for further characteriz ... | 2005 | 15752104 |
| [pharmacokinetic/pharmacodynamic analysis of antibiotic therapy in dentistry and stomatology]. | this study evaluates the efficacy of various antimicrobial treatments for orofacial infections on the basis of pharmacokinetic/pharmacodynamic (pk/pd) criteria. | 2005 | 15757581 |
| bacteria recovered from dental pulp induce apoptosis of lymph node cells. | apoptosis is critical in the pathogenesis of several infectious diseases. the induction of apoptosis was assessed in mouse lymph node cells by four bacteria recovered from infected human dental pulp: gemella morbillorum, clostridium butyricum, fusobacterium nucleatum and bifidobacterium adolescentis. smaller lymph nodes and smaller numbers of cells were observed after experimental dental pulp infection with c. butyricum, suggesting that this bacterium induces cell death. apoptosis was evaluated ... | 2005 | 15770029 |
| portal vein thrombosis despite anticoagulation in a person with diabetes. | 2005 | 15805557 | |
| towards a resolution on the inherent methodological weakness of the "effective number of codons used by a gene". | recently anders fuglsang provided a modified way for calculating n(c) when biased discrepancy is present in a gene [biochem. biophys. res. commun. 317 (2004) 957]. instead of taking the average codon homozygosity for each synonymous family type (as proposed by wright) [gene 87 (1990) 23] fuglsang considered codon homozygosity of each amino acid individually. marsashi and najafabadi [biochem. biophys. res. commun. 324 (2004) 1] in their recent article demonstrated that the readjustment for overes ... | 2005 | 15823544 |
| differences in prevalence of selected bacterial species in primary endodontic infections from two distinct geographic locations. | this study intended to compare the prevalence of 7 putative endodontic pathogens in samples of primary endodontic infections taken from patients of 2 distant geographic locations. | 2005 | 15829891 |
| dna probe identification of bacteria colonizing internal surfaces of the implant-abutment interface: a preliminary study. | currently, there is limited knowledge concerning the specific genus and species of bacteria that may colonize internal surfaces of the implant-abutment interface (iai) of two-stage dental implants. the purpose of this study was to use dna probe analysis to identify those periodontopathic bacteria that may inhabit the internal surfaces and healing abutment screw-threads of the iai of dental implants in situ. | 2005 | 15830645 |