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temperature effect on bacterial azo bond reduction kinetics: an arrhenius plot analysis.studied was the effect of temperature in the range 12-46 degrees c on the rate of bacterial decolorization of the mono-azo dye acid orange 7 by alcaligenes faecalis 6132 and rhodococcus erythropolis 24. with both strains the raise of temperature led to a corresponding raise of decolorization rate better manifested by r. erythropolis. the analysis of the arrhenius plot revealed a break near the middle of the temperature range. the regression analysis showed practically complete identity of the ob ...200817653820
endophytic bacteria in sunflower (helianthus annuus l.): isolation, characterization, and production of jasmonates and abscisic acid in culture medium.this study was designed to isolate and characterize endophytic bacteria from sunflower (helianthus annuus) grown under irrigation and water stress (drought) conditions, to analyze growth of isolated bacteria under drought condition, and to evaluate the ability of bacteria isolated from plants cultivated under drought to produce jasmonates (jas) and abscisic acid (aba). bacteria were isolated from soil samples collected when sunflower plants were at the end of the vegetative stage. a total of 29 ...200717657487
alcaligenes xylosoxidans conjunctivitis.to report a case of chronic unilateral conjunctivitis caused by alcaligenes xylosoxidans.200717667625
alcaligenes xylosoxidans osteomyelitis without trauma in a patient with good's syndrome. 200717693242
influence of different carboxy-terminal mutations on the substrate-, reaction- and enantiospecificity of the arylacetonitrilase from pseudomonas fluorescens ebc191.different members of the nitrilase superfamily (d-carbamoylases, nit-fhit proteins, amidases, cyanide dihydratases and nitrilases) were compared by multiple sequence alignments and a long carboxy-terminal extension (about 50 amino acids) identified in all nitrilases and cyanide dihydratases which was not present in other members of the nitrilase superfamily. the function of this c-terminal part was experimentally analysed in the arylacetonitrilase of pseudomonas fluorescens ebc191 by the constru ...200717693456
control of microorganisms and reduction of biogenic amines in chicken breast and thigh by irradiation and organic acids.the effect of irradiation or organic acid treatment of raw chicken breast and thigh meat to control inoculated microorganisms and the production of biogenic amines (ba) was studied. bacillus cereus, enterobacter cloacae, and alcaligenes faecalis were selected and inoculated into raw ground chicken breast and thigh meat at approximately 10(7) cfu/g. the samples were irradiated at 0, 0.5, 1, and 2 kgy or mixed with a 0.2 m solution of acetic, citric, or lactic acid (1 ml for 10 g of meat sample) f ...200717704395
successful treatment of alcaligenes xylosoxidans in automated peritoneal dialysis-related peritonitis. 200717704455
genomic analysis reveals widespread occurrence of new classes of copper nitrite reductases.recently, the structure of a cu-containing nitrite reductase (nir) from hyphomicrobium denitrificans (hdnir) has been reported, establishing the existence of a new family of cu-nir where an additional type 1 cu (t1cu) containing cupredoxin domain is located at the n-terminus (nojiri et al. in proc. natl. acad. sci. usa 104:4315-4320, 2007). hdnir retains the well-characterised coupled t1cu-type 2 cu (t2cu) core, where the t2cu catalytic site is also built utilising ligands from neighbouring mono ...200717712582
characterization of hbze-encoded gentisate 1,2-dioxygenase from pseudomonas alcaligenes ncimb 9867.pseudomonas alcaligenes ncimb 9867 (strain p25x) is known to synthesize two isofunctional gentisate 1,2-dioxygenases (gdo; ec 1.13.11.4) as well as other enzymes involved in the degradation of xylenols and cresols via the gentisate pathway. the hbze gene encoding what is possibly the strictly inducible gentisate 1,2-dioxygenase ii (gdo-ii) was cloned, overexpressed and purified as a hexahistidine fusion protein from escherichia coli. active recombinant gdo-ii had an estimated molecular mass of 1 ...200717720458
production of poly-beta-hydroxybutyrate (phb) by alcaligenes latus from maple sap.maple sap, an abundant natural product especially in canada, is rich in sucrose and thus may represent an ideal renewable feedstock for the production of a wide variety of value-added products. in the present study, maple sap or sucrose was employed as a carbon source to alcaligenes latus for the production of poly-beta-hydroxybutyrate (phb). in shake flasks, the biomass obtained from both the sap and sucrose were 4.4 +/- 0.5 and 2.9 +/- 0.3 g/l, and the phb contents were 77.6 +/- 1.5 and 74.1 + ...200717724587
microbiological quality of indoor and outdoor swimming pools in greece: investigation of the antibiotic resistance of the bacterial isolates.during 1997-2005, the microbiological quality and susceptibility of bacterial isolates of swimming pool waters were investigated. a total of 462 water samples were collected from three indoor swimming pools (a teaching pool, a competition public pool, a hydrotherapy pool) and two outdoor swimming pools (a hotel semi-public and a residential private pool) in northwestern greece. all water samples were analyzed for the presence of bacteria, protozoa and fungi and susceptibility tests were performe ...200817728184
effect of penicillin on growth of alcaligenes fecalis. 194617748224
alcaligenes abortus from the spinal fluid. 192917758218
polyhydroxybutyrate, a biodegradable thermoplastic, produced in transgenic plants.polyhydroxybutyrate (phb), a high molecular weight polyester, is accumulated as a storage carbon in many species of bacteria and is a biodegradable thermoplastic. to produce phb by genetic engineering in plants, genes from the bacterium alcaligenes eutrophus that encoded the two enzymes required to convert acetoacetyl-coenzyme a to phb were placed under transcriptional control of the cauliflower mosaic virus 35s promoter and introduced into arabidopsis thaliana. transgenic plant lines that conta ...199217787950
a case of native valve infective endocarditis caused by alcaligenes xylosoxidans. 200717868590
phosphorus accumulation by bacteria isolated from a continuous-flow two-sludge system.in this article, polyphosphate-accumulating organisms (paos) from a lab-scale continuous-flow two-sludge system was isolated and identified, the different phosphorus accumulation characteristics of the isolates under anoxic and aerobic conditions were investigated. two kinds of paos were both found in the anoxic zones of the two-sludge system, one of them utilized only oxygen as electron acceptor, and the other one utilized either nitrate or oxygen as electron acceptor. of the total eight isolat ...200717915699
stable copper-nitrosyl formation by nitrite reductase in either oxidation state.nitrite reductase (nir) is an enzyme that uses type 1 and type 2 copper sites to reduce nitrite to nitric oxide during bacterial denitrification. a copper-nitrosyl intermediate is a proposed, yet poorly characterized feature of the nir catalytic cycle. this intermediate is formally described as cu(i)-no+ and is proposed to be formed at the type 2 copper site after nitrite binding and electron transfer from the type 1 copper site. in this study, copper-nitrosyl complexes were formed by prolonged ...200717924665
mechanisms of bacterial resistance to chromium compounds.chromium is a non-essential and well-known toxic metal for microorganisms and plants. the widespread industrial use of this heavy metal has caused it to be considered as a serious environmental pollutant. chromium exists in nature as two main species, the trivalent form, cr(iii), which is relatively innocuous, and the hexavalent form, cr(vi), considered a more toxic species. at the intracellular level, however, cr(iii) seems to be responsible for most toxic effects of chromium. cr(vi) is usually ...200817934697
mycotic femoral aneurysm.after several weeks of fever and chills, a 31-year-old logger developed pain in his right thigh. upon examination a tender, pulsating upper thigh mass was found with a long loud bruit arising from it. severe aortic insufficiency was present; however, blood cultures were negative. an angiogram, captured blood with contrast spewing from the profunda femoral artery to fill a 5 x 10 cm sac. a false aneurysm was diagnosed and resected; numerous gram positive cocci were present in cut sections, but cu ...200717941249
bilateral simultaneous infectious keratitis secondary to contact lens wear: an unusual case report with rare organisms.to report an unusual case of bilateral simultaneous hypopyon corneal ulcer in a contact lens wearer caused by polymicrobial infection with rare organisms.200717993833
purification and characterization of arylmalonate decarboxylase from achromobacter sp. ku1311.we have isolated, purified and characterized arylmalonate decarboxylase (amdase; ec 4.1.1.76). this is an unique enzyme that gives optically pure arylpropionates from the corresponding arylmalonates. recently, we have screened similar enzyme producers from soil samples and succeeded in isolating achromobacter sp. ku1311. the gene encoding the enzyme was cloned and sequenced. the amdase gene consists of 720 nucleotides, which specifies a 240 amino acid protein with a relative molecular mass of 24 ...200718023797
rational redesign of the 4-chlorobenzoate binding site of 4-chlorobenzoate: coenzyme a ligase for expanded substrate range.environmental aromatic acids are transformed to chemical energy in bacteria that possess the requisite secondary pathways. some of these pathways rely on the activation of the aromatic acid by coenzyme a (coa) thioesterification catalyzed by an aromatic acid: coa ligase. adaptation of such pathways to the bioremediation of man-made pollutants such as polychlorinated biphenyl (pcb) and dichlorodiphenyltrichloroethane (ddt) requires that the chlorinated benzoic acid byproduct that is formed be abl ...200718027984
antibacterial activity of guava (psidium guajava l.) and neem (azadirachta indica a. juss.) extracts against foodborne pathogens and spoilage bacteria.the antibacterial activity of guava (psidium guajava) and neem (azadirachta indica) extracts against 21 strains of foodborne pathogens were determined--listeria monocytogenes (five strains), staphylococcus aureus (four strains), escherichia coli o157:h7 (six strains), salmonella enteritidis (four strains), vibrio parahaemolyticus, and bacillus cereus, and five food spoilage bacteria: pseudomonas aeroginosa, p. putida, alcaligenes faecalis, and aeromonas hydrophila (two strains). guava and neem e ...200718041957
deracemization of secondary alcohols through a concurrent tandem biocatalytic oxidation and reduction. 200818072189
preparation of (r)-(-)-mandelic acid and its derivatives from racemates by enantioselective degradation with a newly isolated bacterial strain alcaligenes sp. ecu0401.an enantioselective mandelate-degrading bacterium, alcaligenes sp. ecu0401, was newly isolated from soil. by fed-batch culture, (r)-(-)-mandelic acid was successfully prepared in a 5-l fermenter with 32.8% isolated yield and >99.9% enantiomeric excesses (e.e.) from totally 3.04% (w/v) of racemic mandelic acid after 99 h of biotransformation. the optimal reaction ph and temperature were 6.5 and 30 degrees c, respectively. using the resting cell as a biocatalyst for asymmetric degradation of racem ...200818074154
homology versus analogy: possible evolutionary relationship of immunoglobulins, cupredoxins, and cu,zn-superoxide dismutase.the 'immunoglobulin-like' fold is one of most common structural motifs observed in proteins. this topology is found in more than 80 superfamilies of proteins, including cu,zn-superoxide dismutase (sod) and cupredoxin. evolutionary relationships have not been identified, but may exist. the challenge remains, therefore, of resolving the issue of whether the diverse distribution of the fold is accounted for by divergent evolution of function or convergent evolution of structure following multiple i ...200818080994
lipase expression in pseudomonas alcaligenes is under the control of a two-component regulatory system.preliminary observations in a large-scale fermentation process suggested that the lipase expression of pseudomonas alcaligenes can be switched on by the addition of certain medium components, such as soybean oil. in an attempt to elucidate the mechanism of induction of lipase expression, we have set up a search method for genes controlling lipase expression by use of a cosmid library containing fragments of p. alcaligenes genomic dna. a screen for lipase hyperproduction resulted in the selection ...200818192420
high incidence of plant growth-stimulating bacteria associated with the rhizosphere of wheat grown on salinated soil in uzbekistan.soil salinization is increasing steadily in many parts of the world and causes major problems for plant productivity. under these stress conditions, root-associated beneficial bacteria can help improve plant growth and nutrition. in this study, salt-tolerant bacteria from the rhizosphere of uzbek wheat with potentially beneficial traits were isolated and characterized. eight strains which initially positively affect the growth of wheat plants in vitro were investigated in detail. all eight strai ...200818211262
structural studies on flavohemoglobins.the key three-dimensional features of flavohemoglobins have been unveiled by x-ray crystallographic investigations carried out on the alcaligenes eutrophus and escherichia coli proteins. flavohemoglobins are made of a globin domain fused with a ferredoxin reductase-like fad binding module and display highly conserved sequences in the active sites of both the heme-binding domain and the flavin-binding domain. structural studies are discussed and methodological approaches to the solution of the cr ...200818237633
antibacterial and toxicological evaluation of beta-lactams synthesized by immobilized beta-lactamase-free penicillin amidase produced by alcaligenes sp.search for anti-beta-lactamase and synthesis of newer penicillin were suggested to overcome resistance to penicillin in chemotherapy. it was found that clavulanic acid, an ant-beta-lactamase was ineffective due to its structural modification by bacteria. thus, there is a need for the synthesis of newer pencillins. retro-synthesis was inspired by the success of forward reaction i.e.conversion of penicillin g to 6-aminopenicillanic acid (6-apa) by biological process. in the present study a better ...200718254214
the influence of selected antibiotics on aquatic microorganisms.this paper presents the influence of penicillin, erythromycin, oxytetracycline and streptomycin on aquatic microorganisms isolated from three reservoirs with varied extent of environmental pollution (sulej6w reservoir, zegrze reservoir and vistula river). the experiments were conducted in especially prepared microcosms (aerated 151 aquariums). from among the examined antibiotics streptomycin showed the longest activity in the water environment (27 days), followed by oxytetracycline (22 days). er ...200718254497
extracellular polysaccharides produced by cooling water tower biofilm bacteria and their possible degradation.the extracellular polymers (eps) of biofilm bacteria that can cause heat and mass transfer problems in cooling water towers in the petrochemical industry were investigated. in addition, these microorganisms were screened for their ability to grow and degrade their own eps and the eps of other species. twelve bacteria producing the most eps were isolated from cooling water towers and characterized biochemically by classic and commercial systems. these were species of pseudomonas, burkholderia, ae ...200818256966
modulation of no binding to cytochrome c' by distal and proximal haem pocket residues.we have cloned and expressed the cycp gene encoding cytochrome c' from alcaligenes xylosoxidans and generated mutations in arg-124 and phe-59, residues close to the haem, to probe their involvement in modulating the unusual spin-state equilibrium of the haem fe and the unique proximal mode of binding of no to form a stable five-coordinate adduct. arg-124 is located in the proximal pocket of the haem and forms a hydrogen bond to the stable five-coordinated bound no. phe-59 provides steric hindran ...200818259785
[pseudomonas and alcaligenes--pathogens of the chronic ear infection in clinical cases at the otolaryngology department of the university hospital in krakow].otitis media chronica is the most common disorders encountered in otolaryngology. the great multitude of different bacteria which may couse otitis media constitutes the need for bacteriological diagnostics, especially in cases where classical conservative treatement of the inflammatory process fails.200718260228
[research advances in aerobic denitrifiers].this paper reviewed the varieties and characteristics of aerobic denitrifiers, their action mechanisms, and the factors affecting aerobic denitrification. aerobic denitrifiers mainly include pseudomonas, alcaligenes, paracoccus and bacillus, which are either aerobic or facultative aerobic, and heterotrophic. they can denitrify under aerobic conditions, with the main product being n2o. they can also convert nh4+ -n to gas product. the nitrate reductase which catalyzes the denitrification is perip ...200718260473
assessment of microbial diversity in effluent treatment plants by culture dependent and culture independent approaches.microbial community structure of two distinct effluent treatment plants (etps) of pesticide and pharmaceutical industries was assessed and defined by (i) culture dependent and culture independent approaches on the basis of 16s rrna gene sequencing, (ii) diversity index analysis - operational taxonomic units (otus). a total of 38 and 44 bacterial otus having 85-99% similarity with the closest match in the database were detected among pharmaceutical and pesticide sludge samples, respectively. fift ...200818280146
screening, cloning, expression, and purification of an acidic arylmalonate decarboxylase from enterobacter cloacae ku1313.we have already isolated, purified, and characterized arylmalonate decarboxylases (amdase; ec. 4.1.1.76) from alcaligenes bronchisepticus ku1201 and achromobacter sp. ku1311. these are unique enzymes that give optically pure alpha-arylpropionates from the corresponding alpha-aryl-alpha-methylmalonates. recently, we have further screened novel amdase producers from soil samples under acidic conditions and succeeded in isolating enterobacter cloacae ku1313. the gene encoding the enzyme was cloned ...200818283449
detoxification of toxic heavy metals by marine bacteria highly resistant to mercury.pollution in industrial areas is a serious environmental concern, and interest in bacterial resistance to heavy metals is of practical significance. mercury (hg), cadmium (cd), and lead (pb) are known to cause damage to living organisms, including human beings. several marine bacteria highly resistant to mercury (bhrm) capable of growing at 25 ppm (mg l(-1)) or higher concentrations of mercury were tested during this study to evaluate their potential to detoxify cd and pb. results indicate their ...200818288535
fed batch bioconversion of 2-propanol by a solvent tolerant strain of alcaligenes faecalis entrapped in ca-alginate gel.a gram-negative, rod-shaped, aerobe, capable of converting 2-propanol (isopropanol, ipa) to acetone was isolated from an oil/sump, and identified by 16 s rdna analysis as alcaligenes faecalis. investigations showed this strain to be extremely solvent-tolerant and it was subsequently named st1. in this study, a. faecalis st1 cells were immobilized by entrapment in ca-alginate beads (3 mm in diameter), and used in the bioconversion of high concentration ipa. the biodegradation rates and the corres ...200818293022
nitrate removal efficiency of bacterial consortium (pseudomonas sp. kw1 and bacillus sp. yw4) in synthetic nitrate-rich water.the efficiency of bacterial isolates to reduce nitrate from synthetic nitrate-rich water was tested using a batch scale process. two efficient nitrate reducing bacterial species were isolated from water samples collected from kodaikanal and yercaud lakes. bacterial analysis of the samples revealed the presence of nitrate reducing bacteria belonging to the genera pseudomonas, bacillus, micrococcus and alcaligenes. among the isolates, the consortium of pseudomonas sp. kw1 and bacillus sp. yw4 was ...200818294766
culturable heterotrophic bacteria in seawater and mytilus galloprovincialis from a mediterranean area (northern ionian sea-italy).although mar piccolo of taranto (ionian sea, italy) is one of the most important mytilus galloprovincialis farming areas, data concerning the natural bacterial microbiota of these mussels and their surrounding environment are still scant. this study was carried out seasonally, throughout a year, to determine culturable heterotrophic bacteria both in the water and mussels samples collected at three sampling sites in the northern ionian sea: s. vito, lido gandoli and lido silvana. culturable heter ...200918301997
the enzyme mechanism of nitrite reductase studied at single-molecule level.a generic method is described for the fluorescence "readout" of the activity of single redox enzyme molecules based on förster resonance energy transfer from a fluorescent label to the enzyme cofactor. the method is applied to the study of copper-containing nitrite reductase from alcaligenes faecalis s-6 immobilized on a glass surface. the parameters extracted from the single-molecule fluorescence time traces can be connected to and agree with the macroscopic ensemble averaged kinetic constants. ...200818303118
enzymatic preparation of cefaclor with immobilized penicillin acylase.enzymatic syntheses of cefaclor by immobilized penicillin acylase under kinetic control were carried out. according to the initial reaction rate ratio of synthesis to hydrolysis (vs/vh), penicillin acylase from alcaligenes faecalis was chosen as the suitable catalyst for the synthesis of cefaclor. the reaction conditions, such as temperature, ph, and substrate concentration were investigated based on their vs/vh values. in the process of preparing cefaclor, in situ product removal (ispr) and acy ...200818320464
meningitis caused by alcaligenes xylosoxidans in a patient with hiv/aids.the purpose of the present work was to inform about the first case of meningitis associated to the bacteria alcaligenes xylosoxidans in a patient with hiv/aids. the patient was a 46-year-old male, with the antecedent of have been diagnosed for hiv/ aids, who attended in the hospital universitario de colima, mexico, with fever, shock and meningismus. the study of the cerebrospinal fluid showed pleocytosis, elevated protein levels and hypoglycorrhachia. the culture yielded the presence of alcalige ...200718327475
real-time pcr quantification of pah-ring hydroxylating dioxygenase (pah-rhdalpha) genes from gram positive and gram negative bacteria in soil and sediment samples.real-time pcr based assays were developed to quantify gram positive (gp) and gram negative (gn) bacterial populations that are capable of degrading the polycyclic aromatic hydrocarbons (pah) in soil and sediment samples with contrasting contamination levels. these specific and sensitive real-time pcr assays were based on the quantification of the copy number of the gene that encodes the alpha subunit of the pah-ring hydroxylating dioxygenases (pah-rhdalpha), involved in the initial step of the a ...200818329116
genetic and functional diversity among fluorescent pseudomonads isolated from the rhizosphere of banana.fluorescent pseudomonads from banana rhizospheric soil were isolated and screened for the production of enzymes and hormones such as phosphatase, indole-3-acetic acid (iaa), 1-aminocyclopropane-1-carboxylate (acc) deaminase, protease, and antifungal metabolites. of 95 isolates, 50 (52%) isolates solubilized tri-calcium phosphate (tcp), 63 (66%) isolates produced plant growth hormone iaa, 10 (11%) isolates exhibited acc deaminase, and 23 (24%) isolates produced protease. isolates were screened fo ...200818347847
crystallography with online optical and x-ray absorption spectroscopies demonstrates an ordered mechanism in copper nitrite reductase.nitrite reductases are key enzymes that perform the first committed step in the denitrification process and reduce nitrite to nitric oxide. in copper nitrite reductases, an electron is delivered from the type 1 copper (t1cu) centre to the type 2 copper (t2cu) centre where catalysis occurs. despite significant structural and mechanistic studies, it remains controversial whether the substrates, nitrite, electron and proton are utilised in an ordered or random manner. we have used crystallography, ...200818353369
conserved active site residues limit inhibition of a copper-containing nitrite reductase by small molecules.the interaction of copper-containing dissimilatory nitrite reductase from alcaligenes faecalis s-6 ( afnir) with each of five small molecules was studied using crystallography and steady-state kinetics. structural studies revealed that each small molecule interacted with the oxidized catalytic type 2 copper of afnir. three small molecules (formate, acetate and nitrate) mimic the substrate by having at least two oxygen atoms for bidentate coordination to the type 2 copper atom. these three anions ...200818358002
novel aromatic ring-hydroxylating dioxygenase genes from coastal marine sediments of patagonia.polycyclic aromatic hydrocarbons (pahs), widespread pollutants in the marine environment, can produce adverse effects in marine organisms and can be transferred to humans through seafood. our knowledge of pah-degrading bacterial populations in the marine environment is still very limited, and mainly originates from studies of cultured bacteria. in this work, genes coding catabolic enzymes from pah-biodegradation pathways were characterized in coastal sediments of patagonia with different levels ...200818366740
interactions among phosphate amendments, microbes and uranium mobility in contaminated sediments.the use of sequestering agents for the transformation of radionuclides in low concentrations in contaminated soils/sediments offers considerable potential for environmental cleanup. this study evaluated the influence of three types of phosphate (rock phosphate, biological phosphate, and calcium phytate) and two microbial amendments (alcaligenes piechaudii and pseudomonas putida) on u mobility. all tested phosphate amendments reduced aqueous u concentrations more than 90%, likely due to formation ...200818374392
identification of novel small-molecule histone deacetylase inhibitors by medium-throughput screening using a fluorigenic assay.hdacs (histone deacetylases) are considered to be among the most important enzymes that regulate gene expression in eukaryotic cells. in general, increased levels of histone acetylation are associated with increased transcriptional activity, whereas decreased levels are linked to repression of gene expression. hdacs associate with a number of cellular oncogenes and tumour-suppressor genes, leading to an aberrant recruitment of hdac activity, which results in changes of gene expression, impaired ...200818384290
prevalence of bacterial pathogens and their anti-microbial resistance in tilapia and their pond water in trinidad.in trinidad, tilapia (oreonchromis spp.) is one of the most important fresh water food fish and the number of farms has been increasing annually. a study was conducted in the local tilapia industry to determine the microbial quality of pond water, prevalence of bacterial pathogens and their anti-microbial resistance using the disk diffusion method. seventy-five apparently healthy fish and 15 pond water samples from three of the four commercial tilapia fish farms in the country were processed. th ...200818387142
antimicrobial activity of tissue and associated bacteria from benthic sea anemone stichodactyla haddoni against microbial pathogens.associated bacteria from stichodactyla haddoni are found maximum in tentacle tissues than the body tissue. there are eight associated bacterial species viz., alcaligenes sp, corynebacterium sp, aeromonas sp, sporosarcina sp, renibacterium sp, camobacterium sp1, camobacterium sp2 and salinococcus sp were recorded. the culture extracts from the associated bacterial species showed sensitivity against human bacterial and fungalpathogens. however, the hexane tissue extract of sea anemone showed maxim ...200718405113
postoperative alcaligenes xylosoxidans endophthalmitis: report of two cases.we report two cases of postoperative alcaligenes xylosoxidans endophthalmitis. a 78-year-old woman in good general health developed a. xylosoxidans endophthalmitis one month after an uncomplicated phacoemulsification procedure with posterior chamber intraocular lens. it was performed on her left eye, at another institution. removal of the intraocular lens and capsule was performed because of recurrent inflammation after vitrectomy and intravitreal antibiotic injections. her son, a 55-year-old ma ...200818408852
effect of a tridentate ligand on the structure, electronic structure, and reactivity of the copper(i) nitrite complex: role of the conserved three-histidine ligand environment of the type-2 copper site in copper-containing nitrite reductases.it is postulated that the copper(i) nitrite complex is a key reaction intermediate of copper containing nitrite reductases (cu-nirs), which catalyze the reduction of nitrite to nitric oxide (no) gas in bacterial denitrification. to investigate the structure-function relationship of cu-nir, we prepared five new copper(i) nitrite complexes with sterically hindered tris(4-imidazolyl)carbinols [et-tic = tris(1-methyl-2-ethyl-4-imidazolyl)carbinol and ipr-tic = tris(1-methyl-2-isopropyl-4-imidazolyl) ...200818412340
anaerobic and aerobic degradation of cyanophycin by the denitrifying bacterium pseudomonas alcaligenes strain dip1 and role of three other coisolates in a mixed bacterial consortium.four bacterial strains were isolated from a cyanophycin granule polypeptide (cgp)-degrading anaerobic consortium, identified by 16s rrna gene sequencing, and assigned to species of the genera pseudomonas, enterococcus, clostridium, and paenibacillus. the consortium member responsible for cgp degradation was assigned as pseudomonas alcaligenes strain dip1. the growth of and cgp degradation by strain dip1 under anaerobic conditions were enhanced but not dependent on the presence of nitrate as an e ...200818424548
the structures of alcaligenes faecalis d-3-hydroxybutyrate dehydrogenase before and after nad+ and acetate binding suggest a dynamical reaction mechanism as a member of the sdr family.d-3-hydroxybutyrate dehydrogenase, which catalyzes the reversible reaction between d-3-hydroxybutyrate and acetoacetate, has been classified into the short-chain dehydrogenase/reductase family and is a useful marker in the assay of diabetes mellitus and/or ketoacidosis. the enzyme from alcaligenes faecalis was crystallized in the apo form and in the holo form with acetate as a substrate analogue. the crystal structures of both forms were determined at 2.2 angstroms resolution. the enzyme is a te ...200818453685
biogeochemical processes in the continental slope of bay of bengal: i. bacterial solubilization of inorganic phosphate.microorganisms play a vital role in the biogeochemical cycles of various marine environments, but studies on occurrence and distribution of such bacteria in the marine environment from india are meager. we studied the phosphate solubilizing property of bacteria from the deep sea sediment of bay of bengal, india, to understand their role in phosphorous cycle (and thereby the benthic productivity of the deep sea environment). sediment samples were obtained from 33 stations between 10 degrees 36'n- ...200718457109
nonpuerperal breast infection.we undertook a microbiological study of purulent specimens from women with symptomatic breast abscesses.199518476022
enzymatic synthesis of formic acid from h2 and co2 and production of hydrogen from formic acid.whole cells of alcaligenes eutrophus (as well as isolated p. oxalaticus formate dehydrogenase and a. eutrophus hydrogenase coupled via nad(+) or methyl viologen) have been shown to produce h(2) from formic acid. immobilization of the cells in kappa-carrageenan gel greatly enhances their stability at room temperature. the rate of hydrogen production catalyzed by immobilized a. eutrophus has been studied as a function of the concentrations of the cells and formate and also ph. an inhibition by hig ...198218546098
optimization of biomass productivity and substrate utility of a hydrogen bacterium, alcaligenes hydrogenophilus.optimizations of cell productivity and utility of gaseous substrates were investigated for alcaligenes hydrogenophilus. theoretical analyses for the cell productivity and substrate utility were discussed in the light of experimental data. it was proved from the theoretical analyses that the maximum cell productivity was at the following conditions: d = 0.21 h(-1), r(1) ( = h(2) partial pressure/o(2) partial pressure) = 2.25 and r(2) ( =co(2) partial pressure/o(2) partial pressure) = 0.09, i.e., ...198218546410
anaerobic growth of alcaligenes faecalis var. denitrificans at the expense of ether glycols and nonionic detergents. 198318548680
dectin-1 synergizes with tlr2 and tlr4 for cytokine production in human primary monocytes and macrophages.the beta-glucan receptor dectin-1 and toll-like receptors tlr2 and tlr4 are the main receptors for recognition of candida albicans by the innate immune system. it has been reported that dectin-1 amplifies tlr2-dependent induction of cytokines in mouse models. in the present study we hypothesized that dectin-1 has potent synergistic effects with both tlr2 and tlr4 in human pbmcs and macrophages. human pbmcs and monocyte-derived macrophages were stimulated with curdlan, a linear beta-1,3-glucan-po ...200818549457
enzymatic properties of immobilized alcaligenes faecalis cells with cell-associated beta-glucosidase activity.enzymatic properties of alcaligenes faecalis cells immobilized in polyacrylamide were characterized and compared with those reported for the extracted enzyme, and with those measured for free cells. many of the properties reflected those of the extracted enzyme rather than those measured in the free whole cells prior to immobilization, suggesting cell disruption during immobilization. these properties included the ph activity profile, a slightly broader ph stability profile, and the activation e ...198418553373
kinetics of growth of the hydrogen-oxidizing bacterium alcaligenes eutrophus (atcc 17707) in chemostat culture.the hydrogen-oxidizing bacterium, alcaligenes eutrophus (atcc 17707), was grown in chemostat culture with gas-phase (hydrogen, oxygen, and carbon dioxide) and liquid-phase (mineral nutrients) feedstreams; data were used to generate an analytical form for the specific growth rate equation. model parameters obtained include monod rate parameters for dissolved hydrogen and oxygen gases, yield coefficients, and specific maintenance rates under conditions of hydrogen or oxygen limitations. these valu ...198418553444
characterization of intracellular accumulation of poly-beta-hydroxybutyrate (phb) in individual cells of alcaligenes eutrophus h16 by flow cytometry.poly-beta-hydroxybutyrate (phb) accumulates in individual cells of alcaligenes eutrophus in the form of refractile bodies which alter the light-scattering properties of individual cells. flow cytometry has been applied to measure the distributions of single-cell light-scattering intensity in alc. eutrophus populations during batch cultivation of the organism. these measurements clearly identify heterogeneities in the inoculum which influence the lag interval prior to beginning of exponential gro ...198418553486
production of bioemulsifier by bacillus subtilis, alcaligenes faecalis and enterobacter species in liquid culture.three bacterial strains isolated from waste crude oil were selected due to their capacity of growing in the presence of hydrocarbons and production of bioemulsifier. the genetic identification (pcr of the 16s rdna gene using fd1 and rd1 primers) of these strains showed their affiliation to bacillus subtilis, alcaligenes faecalis and enterobacter sp. these strains were able to emulsify n-octane, toluene, xylene, mineral oils and crude oil, look promising for bioremediation application. finally, c ...200818554901
preparation of l-aspartic acid by means of immobilized alcaligenes metalcaligenes cells.two types of biocatalysts based on immobilized cells of alcaligenes metalcaligenes exhibiting aspartate ammonia-lyase activity (ec 4.3.1.1) were developed for the enzymic preparation of l-aspartic acid from ammonium fumarate. the first type of the biocatalyst consists in individual covalently crosslinked and permeabilized cells(i), while the second type is represented by cell aggregates (ii). for the above preparation, biocatalyst i can be used only discontinuously in a mixed reactor. after term ...198618555429
cloning and sequence analysis of the poly(3-hydroxyalkanoic acid)-synthesis genes of pseudomonas acidophila.pseudomonas acidophila can grow with co2 as a sole carbon source by the possession of a recombinant plasmid that clones genes that confer chemolithoautotrophic growth ability derived from the h2-oxidizing bacterium alcaligenes hydrogenophilus. h2-oxidizing bacteria produce poly(3-hydroxybutyric acid) (phb) from co2, but recombinant p. acidophila can produce the more useful biopolymer poly(3-hydroxyalkanoic acid) (pha). in this study, the pha genes of p. acidophila were cloned and a sequence anal ...199818576003
conversion of food industrial wastes into bioplastics.the usage of plastics in packaging and disposable products, and the generation of plastic waste, have been increasing drastically. broader usage of biodegradable plastics in packaging and disposable products as a solution to environmental problems would heavily depend on further reduction of costs and the discovery of novel biodegradable plastics with improved properties. in the authors' laboratories, various carbohydrates in the growth media, including sucrose, lactic acid, butyric acid, valeri ...199818576025
accumulation of biodegradable copolyesters of 3-hydroxy-butyrate and 3-hydroxyvalerate in alcaligenes eutrophus.biodegradable copolyesters of 3-hydroxybutyrate-co-3-hydroxyvalerate (3hb-3hv) were produced by alcaligenes eutrophus in a two-staged process, namely growth stage and nitrogen-deficient polyester-accumulation stage. when c5 was used as the sole carbon source, the copolyester contained 43 mol % of 3hv. a range of copolyesters with 0-43 mol % of 3hv could be produced by using a medium containing different concentration ratios of butyric acid c4 and c5. t(m) of phb homopolymer was 177.6 degrees c a ...199818576056
observations of metabolite formation and variable yield in thiodiglycol biodegradation process : impact on reactor design.the complete microbial degradation of thiodiglycol (tdg), the primary hydrolysis product of sulfur mustard, by alcaligenes xylosoxydans ssp. xylosoxydans (sh91) was accomplished in laboratory-scale stirredtank reactors. an andrews substrate inhibition model was used to describe the cell growth. the yield factor was not constant, but a relationship with initial substrate concentration has been developed. using a substrate-inhibition and variable-yield kinetic model, we can describe the cell growt ...199718576129
an immobilized hydrogenase from alcaligenes eutrophus h-16.alcaligenes eutrophus h-16 was grown in continuous culture under conditions which induced hydrogenase production. the hydrogenase enzyme was extracted, partially purified and immobilized on porous glass. this enzyme was then studied both in solution and in immobilized form as a possible candidate for a number of industrial applications. it proved to have a stability (storage and operational) which was highly temperature dependent. temperatures near freezing caused the enzyme to retain its activi ...198818584750
crystallization and preliminary x-ray diffraction experiments of arylmalonate decarboxylase from alcaligenes bronchisepticus.arylmalonate decarboxylase catalyses the enantioselective decarboxylation of alpha-aryl-alpha-methylmalonates to produce optically pure alpha-arylpropionates. the enzyme was crystallized with ammonium sulfate under alkaline ph conditions with the aim of understanding the mechanism of the enantioselective reaction. x-ray diffraction data collected to a resolution of 3.0 a at cryogenic temperature showed that the crystals belonged to the orthorhombic space group p2(1)2(1)2(1), with unit-cell param ...200818607088
cybernetic model for synthesis of poly-beta-hydroxybutyric acid in alcaligenes eutrophus.the pathway of poly-beta-hydroxybutyric acid (phb) exhibits a mode of transcriptional control induced by environmental stress. a new cybernetic model for coordinated regulation of stress-induced metabolism was developed to predict the growth and the synthesis of phb in alcaligenes eutrophus. a plausible objective for this control is optimization of acetyl-coa utilization so that the cells have a high degree of flexibility in their catabolism. the state equation for key protein synthesis was assu ...199418615515
production of poly(3-hydroxybutyric acid) by fed-batch culture of alcaligenes eutrophus with glucose concentration control.alcaligenes eutrophus ncimb 11599 was cultivated to produce poly(3-hydroxybutyric acid) (phb) from glucose by the automatic fed-batch culture technique. the glucose concentration of the culture broth was controlled at 10 to 20 g/l by two methods: using exit gas data obtained from a mass spectrometer and using an on-line glucose analyzer. the effect of ammonium limitation on phb synthesis at different culture phases was studied. the final cell concentration, phb concentration, and phb productivit ...199418615882
effect of alcohol feeding mode on the biosynthesis of poly (3-hydroxybutyrate-co-3-hyroxyvalerate).an alcohol utilizing alcaligenes eutrophus produced poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (phbv) copolymer under phosphate limitation. fermentation was performed for 42-46 h in a computer-controlled 5-l working volume fed-batch fermentor using ethanol and propanol as carbon sources. the culture experienced phosphate limitation in approximately 19 h. when propanol was used as a sole carbon source, 24 g/l of copolymer with 36.5 mol % of hydroxyvalerate (hv) was produced at a polymer yield o ...199418618642
comparison of recombinant escherichia coli strains for synthesis and accumulation of poly-(3-hydroxybutyric acid) and morphological changes.a stable high-copy-number plasmid psyl105 containing the alcaligenes eutrophus polyhydroxyalkanoic acid (pha) biosynthesis genes was constructed. this plasmid was transferred to seven escherichia coli strains (k12, b, w, xl1-blue, jm109, dh5alpha, and hb101), which were subsequently compared for their ability to synthesize and accumulate ploy- (3-hydroxybutyric acid) (phb). growth of recombinant cells and phb synthesis were investigated in detail in luria-bertani (lb) medium containing 20 g/l gl ...199418618646
optimization of microbial poly(3-hydroxybutyrate) recover using dispersions of sodium hypochlorite solution and chloroform.optimization was carried out for the recovery of microbiol poly(3-hydroxybutyrate) (phb) from alcaligenes eutrophus. this process involved the use of a dispersion made of sodium hypochlorite solution and chloroform. the dispersion enabled us to take advantage of both differential digestion by hypochlorite and solvent extraction by chloroform. the phb recovery (%) from cell powder was maximized using a 30% hypochlorite concentration, a 90-min treatment time, and a 1:1 (v/v) chloroform-to-aqueous- ...199418618692
production of poly(d-3-hydroxybutyrate) from co(2), h(2), and o(2) by high cell density autotrophic cultivation of alcaligenes eutrophus.hydrogen-oxidizing bacterium, alcaligenes eutrophus autotrophically produces biodegradable plastic material, poly(d-3-hydroxybutyrate), p(3hb), from carbon dioxide, hydrogen, and oxygen. in autotrophic cultivation of the microorganism, it is essential to eliminate possible occurrence of gas explosions from the fermentation process. we developed a bench-plant scale, recycled-gas, closed-circuit culture system equipped with several safety features to perform autotrophic cultivation of a. eutrophus ...199518623147
bacterial polyhydroxyalkanoates.polyhydroxyalkanoates (phas) are polyesters of hydroxyalkanoates (has) synthesized by numerous bacteria as intracellular carbon and energy storage compounds and accumulated as granules in the cytoplasm of cells. more than 80 has have been detected as constituents of phas, which allows these thermoplastic materials to have various mechanical properties resembling hard crystalline polymer or elastic rubber depending on the incorporated monomer units. even though phas have been recognized as good c ...199618623547
synthesis of poly-(3-hydroxybutyrate-co-3-hydroxyvalerate) by recombinant escherichia coli.several recombinant escherichia coli strains, including xl1-blue, jm109, hb101, and dh5alpha harboring a stable high-copynumber plasmid psyl105 containing the alcaligenes eutrophus polyhydroxyalkanoate (pha) biosynthesis genes were constructed. these recombinant strains were examined for their ability to synthesize and accumulate poly-(3-hydroxybutyrate-co-3-hydroxyvalerate) [p(3hb-co-3hv)] copolymer from glucose and either propionate or valerate. all recombinant e. coli strains could synthesize ...199618623611
[isolation and identification of imazethapyr degradable bacteria and its degradation characteristics].one strain of imazethapyr degradable bacteria was isolated from the mixture of imazethapyr production factory's sludge and the soil that was contaminated with imazethapyr for a long time. the strain could degrade imazethapyr more than 90% within 72 h when it grew in 500 mg/l imazethapyr condition. when ph was 5, the bacteria could degrade all the imazethapyr of 500 mg/l in 72 h, but when ph was 8 and 9, only 50% imazethapyr was degraded which showed acid condition was more compatible than that o ...200818624207
increased phb productivity by high-cell-density fed-batch culture of alcaligenes latus, a growth-associated phb producer.alcaligenes latus, a growth-associated phb producer, was cultivated by a ph-stat modal fed-batch culture technique to attain high phb productivity. both sucrose solution and inorganic medium were fed in conjunction with the supply of ammonia solution which serves as a nitrogen source and as a means of ph control. compositions of the inorganic medium were formulated by elemental analysis of a. latus cell mass. the effect on inoculum size was examined to reduce culture time. high concentrations of ...199618626935
regulatory effects of cellular nicotinamide nucleotides and enzyme activities on poly(3-hydroxybutyrate) synthesis in recombinant escherichia coli.regulatory roles of nicotinamide nucleotides and three key enzymes, beta-ketothiolase (kt), nadph-dependent acetoacetyl-coa reductase (aar), and citrate synthase (cs), on poly(3-hydroxybutyrate) (phb) synthesis in recombinant escherichia coli harboring a plasmid containing the alcaligenes eutrophus polyhydroxyalkanoate (pha) biosynthesis genes were examined. cells were grown in various media and were subsequently compared for phb concentration, phb content, the activities of the key enzymes, and ...199618629949
effects of rheological change by addition of carboxymethylcellulose in culture media of an air-lift fermentor on poly-d-3-hydroxybutyric acid productivity in autotrophic culture of hydrogen-oxidizing bacterium, alcaligenes eutrophus.the effects of rheological change by addition of sodium carboxymethylcellulose (cmc) to culture medium in an air-lift-type fermentor on autotrophic production of poly-(d-3-hydroxybutyric acid) [p(3hb)] by two-stage culture of alcaligenes eutrophus is investigated. addition of 0.05% cmc increased p(3hb) production rate during the p(3hb) accumulation phase to twice that of the control culture. it was thought that addition of a small amount of cmc was beneficial for production of p(3hb) employing t ...199718634049
novel endo-alpha-n-acetylgalactosaminidases with broader substrate specificity.in an effort to identify novel endo-alpha-n-acetylgalactosaminidases (endo-alpha-galnacases), four potential genes were cloned. three of the expressed proteins engef from enterococcus faecalis, engpa from propionibacterium acnes, and engcp from clostridium perfringens were purified and characterized. their substrate specificity was investigated and compared to the commercially available endo-alpha-galnacases from streptococcus pneumoniae (engsp) and alcaligenes sp. (engal). all enzymes were incu ...200818635885
production of poly(3-hydroxybutyrate) by high cell density fed-batch culture of alcaligenes eutrophus with phospate limitation.high cell density fed-batch fermentation of alcaligenes eutrophus was carried out for the production of poly(3-hydroxybutyrate) (phb) in a 60-l fermentor. during the fermentation, ph was controlled with nh(4)oh solution and phb accumulation was induced by phosphate limitation instead of nitrogen limitation. the glucose feeding was controlled by monitoring dissolved oxygen (do) concentration and glucose concentration in the culture broth. the glucose concentration fluctuated within the range of 0 ...199718636441
application of self-cycling fermentation technique to the production of poly-beta-hydroxybutyrate.the production of poly-beta-hydroxybutyrate (phb) by alcaligenes eutrophus dsm 545 in a cyclone bioreactor was compared using various culture methods: batch, fed-batch, and self-cycling fermentation (scf) with and without extended periods of nutrient deprivation. scf is a semi-continuous method that results in a nutrient limitation for every successive generation of cells and, therefore, may have advantages for products whose formation follow secondary metabolite kinetics. use of the scf techniq ...199718636592
arsenite oxidation by alcaligenes sp. strain rs-19 isolated from arsenic-contaminated mines in the republic of korea.arsenite [as(iii)]-oxidizing bacteria play important roles in reducing arsenic [as] toxicity and mobility in as-contaminated areas. as-resistant bacteria were isolated from the soils of two abandoned mines in the republic of korea. the isolated bacteria showed relatively high resistances to as(iii) up to 26 mm. the pcr-based 16s rrna analysis revealed that the isolated as-resistant bacteria were close relatives to serratia marcescensa, pseudomonas putida, pantoea agglomerans, and alcaligenes sp. ...200918642094
experimental optimization of fed-batch culture for poly-beta-hydroxybutyric acid production.the optimal feed rate profiles of glucose and ammonium hydroxide were calculated using a proposed model, and implemented for the production of poly-beta-hydroxybutyric acid (phb) by alcaligenes eutrophus. by implementing these optimal feed rates with a high glucose feed concentration of 700 g/l and an ammonium hydroxide concentration of 7%(w/w), it was possible to achieve a high final cell concentration of 141 g/l and a high phb concentration of 105 g/l in 40 h of fed-batch operation. the phb pr ...199718642342
reduction of petroleum hydrocarbons and toxicity in refinery wastewater by bioremediation.the aim of the study was to investigate petroleum waste remediation and toxicity reduction by five bacterial strains: ralstonia picketti srs (bp-20), alcaligenes piechaudii srs (czor l-1b), bacillus subtilis (i'-1a), bacillus sp. (t-1), and bacillus sp. (t'-1), previously isolated from petroleum-contaminated soils. petroleum hydrocarbons were significantly degraded (91%) by the mixed bacterial cultures in 30 days (reaching up to 29% in the first 72 h). similarly, the toxicity of the biodegraded ...200818663400
characterization of the proteins of bordetella isolates from turkeys in the uk by polyacrylamide gel electrophoresis.the proteins of seven bordetella bronchiseptica-like isolates were examined by polyacrylamide gel electrophoresis (page). they were compared with three similar isolates from overseas, one of which is the type strain of bordetella avium, and with bordetella bronchiseptica nctc 452t and alcaligenes faecalis nctc 415. apart from alcaligenes faecalis, there was strong homology between the isolates suggesting their close relationship, but minor differences were apparent, too. the significance of thes ...199218670923
rapid identification of bordetella avium and related organisms on the basis of their cellular carbohydrate patterns.whole cell hydrolysates of bordetella avium, b. bronchiseptica, b. pertussis, b. parapertussis, alcaligenes faecalis, a. xylosoxidans ssp. denitrificans, a. xylosoxidans ssp. xylosoxidans, and a. piechaudii were analysed for their cellular carbohydrates by capillary gas-chromatography of per-acetylated aldononitriles and o-methyloximes, respectively. all alcaligenaceae species could be discriminated on the basis of their carbohydrate profiles and a few conventional features. taxometric evaluatio ...199118680060
two similar enhanced root-colonizing pseudomonas strains differ largely in their colonization strategies of avocado roots and rosellinia necatrix hyphae.pseudomonas alcaligenes avo73 and pseudomonas pseudoalcaligenes avo110 were selected previously as efficient avocado root tip colonizers, displaying in vitro antagonism towards rosellinia necatrix, causal agent of avocado white root rot. despite the higher number of antagonistic properties shown in vitro by avo73, only avo110 demonstrated significant protection against avocado white root rot. as both strains are enhanced root colonizers, and as colonization is crucial for the most likely biocont ...200818684119
efficacy of intraoperative vancomycin in irrigating solutions on aqueous contamination during phacoemulsification.to study the efficacy of adding vancomycin in irrigating solutions, in comparison to topical antibiotic given preoperatively for a day, during phacoemulsification, in reducing the anterior chamber (ac) contamination.200818711269
[isolation and characterization of a new glyphosate-resistant strain from extremely polluted environment].to isolate and characterize a glyphosate-resistant strain from extremely polluted environment.200818720850
on-line optical and x-ray spectroscopies with crystallography: an integrated approach for determining metalloprotein structures in functionally well defined states.x-ray-induced redox changes can lead to incorrect assignments of the functional states of metals in metalloprotein crystals. the need for on-line monitoring of the status of metal ions (and other chromophores) during protein crystallography experiments is of growing importance with the use of intense synchrotron x-ray beams. significant efforts are therefore being made worldwide to combine different spectroscopies in parallel with x-ray crystallographic data collection. here the implementation a ...200818728313
the characterisation of bordetella/alcaligenes-like organisms and their effects on turkey poults and chicks.eight isolates of the bordetella or alcaligenes-like organisms associated with turkey rhino-tracheitis were examined. five of these isolates had been recovered from the united kingdom and three were foreign isolates. four of the uk isolates came from flocks with mild respiratory disease. the fifth isolate came from birds with no respiratory signs and this appears to be the first report of the recovery of bordetella/alcaligenes from apparently normal turkeys. the field isolates and type strains a ...198618766500
identification of bordetella avium sp. nov. by the api 20 ne system.the reactions of 47 bordetella avium strains in the api 20 ne micro-test system were compared to those of the species bordetella bronchiseptica, alcaligenes faecalis and alcaligenes denitrificans. the api 20 ne microtest system allowed separation between b. avium and the other species of gram-negative nonfermentative rods investigated.198618766558
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