Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
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| lamp assay and rapid sample preparation method for on-site detection of flavescence dorée phytoplasma in grapevine. | in europe the most devastating phytoplasma associated with grapevine yellows (gy) diseases is a quarantine pest, flavescence dorée (fdp), from the 16srv taxonomic group. the on-site detection of fdp with an affordable device would contribute to faster and more efficient decisions on the control measures for fdp. therefore, a real-time isothermal lamp assay for detection of fdp was validated according to the eppo standards and miqe guidelines. the lamp assay was shown to be specific and extremely ... | 2014 | 26146413 |
| lamp assay and rapid sample preparation method for on-site detection of flavescence dorée phytoplasma in grapevine. | in europe the most devastating phytoplasma associated with grapevine yellows (gy) diseases is a quarantine pest, flavescence dorée (fdp), from the 16srv taxonomic group. the on-site detection of fdp with an affordable device would contribute to faster and more efficient decisions on the control measures for fdp. therefore, a real-time isothermal lamp assay for detection of fdp was validated according to the eppo standards and miqe guidelines. the lamp assay was shown to be specific and extremely ... | 2014 | 26146413 |
| two different evolutionary lines of filamentous phages in ralstonia solanacearum: their effects on bacterial virulence. | the integration and excision of various filamentous phage genomes into and out of their host chromosomes occurs by site-specific recombination. the mechanisms proposed for these events include reactions mediated by phage-encoded recombinases and host recombination systems. site-specific integration of filamentous phages plays a vital role in a variety of biological functions of the host, such as phase variation of certain pathogenic bacterial virulence factors. the importance of these filamentou ... | 2015 | 26150828 |
| the alkb family of fe(ii)/α-ketoglutarate-dependent dioxygenases: repairing nucleic acid alkylation damage and beyond. | the alkb family of fe(ii)- and α-ketoglutarate-dependent dioxygenases is a class of ubiquitous direct reversal dna repair enzymes that remove alkyl adducts from nucleobases by oxidative dealkylation. the prototypical and homonymous family member is an escherichia coli "adaptive response" protein that protects the bacterial genome against alkylation damage. alkb has a wide variety of substrates, including monoalkyl and exocyclic bridged adducts. nine mammalian alkb homologs exist (alkbh1-8, fto), ... | 2015 | 26152727 |
| type ii secretion-dependent degradative and cytotoxic activities mediated by stenotrophomonas maltophilia serine proteases stmpr1 and stmpr2. | stenotrophomonas maltophilia is an emerging opportunistic pathogen that primarily causes pneumonia and bacteremia in immunocompromised individuals. we recently reported that s. maltophilia strain k279a encodes the xps type ii secretion system and that xps promotes rounding, actin rearrangement, detachment, and death in the human lung epithelial cell line a549. here, we show that xps-dependent cell rounding and detachment occur with multiple human and murine cell lines and that serine protease in ... | 2015 | 26169274 |
| the sulfur/sulfonates transport systems in xanthomonas citri pv. citri. | the xanthomonas citri pv. citri (x. citri) is a phytopathogenic bacterium that infects different species of citrus plants where it causes canker disease. the adaptation to different habitats is related to the ability of the cells to metabolize and to assimilate diverse compounds, including sulfur, an essential element for all organisms. in escherichia coli, the necessary sulfur can be obtained by a set of proteins whose genes belong to the cys regulon. although the cys regulon proteins and their ... | 2015 | 26169280 |
| bacteria murmur: application of an acoustic biosensor for plant pathogen detection. | a multi-targeting protocol for the detection of three of the most important bacterial phytopathogens, based on their scientific and economic importance, was developed using an acoustic biosensor (the quartz crystal microbalance) for dna detection. acoustic detection was based on a novel approach where dna amplicons were monitored and discriminated based on their length rather than mass. experiments were performed during real time monitoring of analyte binding and in a direct manner, i.e. without ... | 2015 | 26177507 |
| the dsf family of cell-cell signals: an expanding class of bacterial virulence regulators. | many pathogenic bacteria use cell-cell signaling systems involving the synthesis and perception of diffusible signal molecules to control virulence as a response to cell density or confinement to niches. bacteria produce signals of diverse structural classes. signal molecules of the diffusible signal factor (dsf) family are cis-2-unsaturated fatty acids. the paradigm is cis-11-methyl-2-dodecenoic acid from xanthomonas campestris pv. campestris (xcc), which controls virulence in this plant pathog ... | 2015 | 26181439 |
| identification of bradyrhizobium elkanii genes involved in incompatibility with soybean plants carrying the rj4 allele. | symbioses between leguminous plants and soil bacteria known as rhizobia are of great importance to agricultural production and nitrogen cycling. while these mutualistic symbioses can involve a wide range of rhizobia, some legumes exhibit incompatibility with specific strains, resulting in ineffective nodulation. the formation of nodules in soybean plants (glycine max) is controlled by several host genes, which are referred to as rj genes. the soybean cultivar barc2 carries the rj4 gene, which re ... | 2015 | 26187957 |
| secretion systems and signal exchange between nitrogen-fixing rhizobia and legumes. | the formation of symbiotic nitrogen-fixing nodules on the roots and/or stem of leguminous plants involves a complex signal exchange between both partners. since many microorganisms are present in the soil, legumes and rhizobia must recognize and initiate communication with each other to establish symbioses. this results in the formation of nodules. rhizobia within nodules exchange fixed nitrogen for carbon from the legume. symbiotic relationships can become non-beneficial if one partner ceases t ... | 2015 | 26191069 |
| stress-responsive expression, subcellular localization and protein-protein interactions of the rice metacaspase family. | metacaspases, a class of cysteine-dependent proteases like caspases in animals, are important regulators of programmed cell death (pcd) during development and stress responses in plants. the present study was focused on comprehensive analyses of expression patterns of the rice metacaspase (osmc) genes in response to abiotic and biotic stresses and stress-related hormones. results indicate that members of the osmc family displayed differential expression patterns in response to abiotic (e.g., dro ... | 2015 | 26193260 |
| sol-gel encapsulation of binary zn(ii) compounds in silica nanoparticles. structure-activity correlations in hybrid materials targeting zn(ii) antibacterial use. | in the emerging issue of enhanced multi-resistant properties in infectious pathogens, new nanomaterials with optimally efficient antibacterial activity and lower toxicity than other species attract considerable research interest. in an effort to develop such efficient antibacterials, we a) synthesized acid-catalyzed silica-gel matrices, b) evaluated the suitability of these matrices as potential carrier materials for controlled release of znso4 and a new zn(ii) binary complex with a suitably des ... | 2015 | 26198972 |
| a single-step purification of cauliflower lysozyme and its dual role against bacterial and fungal plant pathogens. | a novel lysozyme from cauliflower was purified in a single step, for the first time, using sephadex g100 column chromatography. the purified lysozyme exhibited a homogenized single band in sodium dodecyl sulfate polyacrylamide gel electrophoresis (sds-page), and its molecular mass was calculated to be 22.0 kda. the purified lysozyme showed activity between 30 to 60 °c with 40 °c as the optimum temperature for its maximal activity. although the purified lysozyme was functional at ph ranges betwee ... | 2015 | 26208688 |
| novel glucose-1-phosphatase with high phytase activity and unusual metal ion activation from soil bacterium pantoea sp. strain 3.5.1. | phosphorus is an important macronutrient, but its availability in soil is limited. many soil microorganisms improve the bioavailability of phosphate by releasing it from various organic compounds, including phytate. to investigate the diversity of phytate-hydrolyzing bacteria in soil, we sampled soils of various ecological habitats, including forest, private homesteads, large agricultural complexes, and urban landscapes. bacterial isolate pantoea sp. strain 3.5.1 with the highest level of phytas ... | 2015 | 26209662 |
| quantitative disease resistance to the bacterial pathogen xanthomonas campestris involves an arabidopsis immune receptor pair and a gene of unknown function. | although quantitative disease resistance (qdr) is a durable and broad-spectrum form of resistance in plants, the identification of the genes underlying qdr is still in its infancy. rks1 (resistance related kinase1) has been reported recently to confer qdr in arabidopsis thaliana to most but not all races of the bacterial pathogen xanthomonas campestris pv. campestris (xcc). we therefore explored the genetic bases of qdr in a. thaliana to diverse races of x. campestris (xc). a nested genome-wide ... | 2016 | 26212639 |
| full genome sequence analysis of two isolates reveals a novel xanthomonas species close to the sugarcane pathogen xanthomonas albilineans. | xanthomonas albilineans is the bacterium responsible for leaf scald, a lethal disease of sugarcane. within the xanthomonas genus, x. albilineans exhibits distinctive genomic characteristics including the presence of significant genome erosion, a non-ribosomal peptide synthesis (nrps) locus involved in albicidin biosynthesis, and a type 3 secretion system (t3ss) of the salmonella pathogenicity island-1 (spi-1) family. we sequenced two x. albilineans-like strains isolated from unusual environments ... | 2015 | 26213974 |
| challenges and perspectives in combinatorial assembly of novel exopolysaccharide biosynthesis pathways. | because of their rheological properties various microbial polysaccharides are applied as thickeners and viscosifiers both in food and non-food industries. a broad variety of microorganisms secrete structurally diverse exopolysaccharides (eps) that contribute to their surface attachment, protection against abiotic or biotic stress factors, and nutrient gathering. theoretically, a massive number of eps structures are possible through variations in monosaccharide sequences, condensation linkages an ... | 2015 | 26217319 |
| postharvest survival of porcine sapovirus, a human norovirus surrogate, on phytopathogen-infected leafy greens. | leafy greens are increasingly being recognized as an important vehicle for human noroviruses (hunov), which cause recurring gastroenteritis outbreaks. leafy greens often become infected by phytopathogens in the field, which may cause symptoms on the edible parts. whether plant pathogen infections enhance the survival of hunov on leafy greens is unknown. lettuce and spinach plants were infected with a bacterium, xanthomonas campestris pv. vitians strain 701a, and with cucumber mosaic virus strain ... | 2015 | 26219360 |
| heparin and related polysaccharides: synthesis using recombinant enzymes and metabolic engineering. | glycosaminoglycans are linear anionic polysaccharides that exhibit a number of important biological and pharmacological activities. the two most prominent members of this class of polysaccharides are heparin/heparan sulfate and the chondroitin sulfates (including dermatan sulfate). these polysaccharides, having complex structures and polydispersity, are biosynthesized in the golgi of most animal cells. the chemical synthesis of these glycosaminoglycans is precluded by their structural complexity ... | 2015 | 26219501 |
| lindgomycin, an unusual antibiotic polyketide from a marine fungus of the lindgomycetaceae. | an unusual polyketide with a new carbon skeleton, lindgomycin (1), and the recently described ascosetin (2) were extracted from mycelia and culture broth of different lindgomycetaceae strains, which were isolated from a sponge of the kiel fjord in the baltic sea (germany) and from the antarctic. their structures were established by spectroscopic means. in the new polyketide, two distinct domains, a bicyclic hydrocarbon and a tetramic acid, are connected by a bridging carbonyl. the tetramic acid ... | 2015 | 26225984 |
| burkholderia diffusible signal factor signals to francisella novicida to disperse biofilm and increase siderophore production. | in many bacteria, the ability to modulate biofilm production relies on specific signaling molecules that are either self-produced or made by neighboring microbes within the ecological niche. we analyzed the potential interspecies signaling effect of the burkholderia diffusible signal factor (bdsf) on francisella novicida, a model organism for francisella tularensis, and demonstrated that bdsf both inhibits the formation and causes the dispersion of francisella biofilm. specificity was demonstrat ... | 2015 | 26231649 |
| the pepper phosphoenolpyruvate carboxykinase capepck1 is involved in plant immunity against bacterial and oomycete pathogens. | phosphoenolpyruvate carboxykinase, a member of the lyase family, is involved in the metabolic pathway of gluconeogenesis in organisms. although the major function of pepck in gluconeogenesis is well established, it is unclear whether this enzyme is involved in plant immunity. here, we isolated and identified the pepper (capsicum annuum) pepck (capepck1) gene from pepper leaves infected with xanthomonas campestris pv. vesicatoria (xcv). capepck1 was strongly expressed in pepper leaves during the ... | 2015 | 26233534 |
| noisy neighbourhoods: quorum sensing in fungal-polymicrobial infections. | quorum sensing was once considered a way in which a species was able to sense its cell density and regulate gene expression accordingly. however, it is now becoming apparent that multiple microbes can sense particular quorum-sensing molecules, enabling them to sense and respond to other microbes in their neighbourhood. such interactions are significant within the context of polymicrobial disease, in which the competition or cooperation of microbes can alter disease progression. fungi comprise a ... | 2015 | 26243526 |
| technology and potential applications of probiotic encapsulation in fermented milk products. | fermented milk products containing probiotics and prebiotics can be used in management, prevention and treatment of some important diseases (e.g., intestinal- and immune-associated diseases). microencapsulation has been used as an efficient method for improving the viability of probiotics in fermented milks and gastrointestinal tract. microencapsulation of probiotic bacterial cells provides shelter against adverse conditions during processing, storage and gastrointestinal passage. important chal ... | 2014 | 26243890 |
| technology and potential applications of probiotic encapsulation in fermented milk products. | fermented milk products containing probiotics and prebiotics can be used in management, prevention and treatment of some important diseases (e.g., intestinal- and immune-associated diseases). microencapsulation has been used as an efficient method for improving the viability of probiotics in fermented milks and gastrointestinal tract. microencapsulation of probiotic bacterial cells provides shelter against adverse conditions during processing, storage and gastrointestinal passage. important chal ... | 2014 | 26243890 |
| role of quorum sensing in bacterial infections. | quorum sensing (qs) is cell communication that is widely used by bacterial pathogens to coordinate the expression of several collective traits, including the production of multiple virulence factors, biofilm formation, and swarming motility once a population threshold is reached. several lines of evidence indicate that qs enhances virulence of bacterial pathogens in animal models as well as in human infections; however, its relative importance for bacterial pathogenesis is still incomplete. in t ... | 2015 | 26244150 |
| xanthomonas campestris cell-cell signalling molecule dsf (diffusible signal factor) elicits innate immunity in plants and is suppressed by the exopolysaccharide xanthan. | several secreted and surface-associated conserved microbial molecules are recognized by the host to mount the defence response. one such evolutionarily well-conserved bacterial process is the production of cell-cell signalling molecules which regulate production of multiple virulence functions by a process known as quorum sensing. here it is shown that a bacterial fatty acid cell-cell signalling molecule, dsf (diffusible signal factor), elicits innate immunity in plants. the dsf family of signal ... | 2015 | 26248667 |
| progress in the development of gelling agents for improved culturability of microorganisms. | gelling agents are required for formulating both solid and semisolid media, vital for the isolation of microorganisms. gelatin was the first gelling agent to be discovered but it soon paved the way for agar, which has far superior material qualities. source depletion, issues with polymerase-chain-reaction and inability to sustain extermophiles etc., necessitate the need of other gelling agents. many new gelling agents, such as xantham gum, gellan gum, carrageenan, isubgol, and guar gum have been ... | 2015 | 26257708 |
| new insights into the biological role of the osmoregulated periplasmic glucans in pathogenic and symbiotic bacteria. | this review emphasizes the biological roles of the osmoregulated periplasmic glucans (opgs). osmoregulated periplasmic glucans occur in almost all α-, β- and γ-proteobacteria. this polymer of glucose is required for full virulence. the roles of the opgs are complex and vary depending on the species. here, we outline the four major roles of the opgs through four different pathogenic and one symbiotic bacterial models (dickeya dadantii, salmonella enterica, pseudomonas aeruginosa, brucella abortus ... | 2015 | 26265506 |
| l-citrulline production by metabolically engineered corynebacterium glutamicum from glucose and alternative carbon sources. | l-citrulline plays an important role in human health and nutrition and is an intermediate of the l-arginine biosynthetic pathway. l-citrulline is a by-product of l-arginine production by corynebacterium glutamicum. in this study, c. glutamicum was engineered for overproduction of l-citrulline as major product without l-arginine being produced as by-product. to this end, l-arginine biosynthesis was derepressed by deletion of the arginine repressor gene argr and conversion of l-citrulline towards ... | 2014 | 26267114 |
| tlc-direct bioautography as a bioassay guided method for investigation of antibacterial compounds in hypericum perforatum l. | fast high-throughput tlc-direct bioautography (db) is an effect-directed analysis method that enables searching for biologically active (e.g., antimicrobial) substances in complex mixtures like plant extracts. the principle of the method is that separation and detection of biological properties of given mixture components is performed directly on a tlc plate. in searching for antibacterial activity, the developed plate is immersed in a bacterial broth, and bacteria grow directly on its layer dur ... | 2015 | 26268984 |
| xanthomonas campestris expansin-like x domain is a structurally disordered beta-sheet macromolecule capable of synergistically enhancing enzymatic efficiency of cellulose hydrolysis. | to biochemically characterize an expansin-like x protein domain from xanthomonas campestris (xcexlx1) and to study its synergy with cellulases in cellulose depolymerization. | 2015 | 26272392 |
| from genome to structure and back again: a family portrait of the transcarbamylases. | enzymes in the transcarbamylase family catalyze the transfer of a carbamyl group from carbamyl phosphate (cp) to an amino group of a second substrate. the two best-characterized members, aspartate transcarbamylase (atcase) and ornithine transcarbamylase (otcase), are present in most organisms from bacteria to humans. recently, structures of four new transcarbamylase members, n-acetyl-l-ornithine transcarbamylase (aotcase), n-succinyl-l-ornithine transcarbamylase (sotcase), ygew encoded transcarb ... | 2015 | 26274952 |
| comparative genomics of environmental and clinical stenotrophomonas maltophilia strains with different antibiotic resistance profiles. | stenotrophomonas maltophilia, a ubiquitous gram-negative γ-proteobacterium, has emerged as an important opportunistic pathogen responsible for nosocomial infections. a major characteristic of clinical isolates is their high intrinsic or acquired antibiotic resistance level. the aim of this study was to decipher the genetic determinism of antibiotic resistance among strains from different origins (i.e., natural environment and clinical origin) showing various antibiotic resistance profiles. to th ... | 2015 | 26276674 |
| functional analysis of a bacitracin resistance determinant located on icecp1, a novel tn916-like element from a conjugative plasmid in clostridium perfringens. | bacitracins are mixtures of structurally related cyclic polypeptides with antibiotic properties. they act by interfering with the biosynthesis of the bacterial cell wall. in this study, we analyzed an avian necrotic enteritis strain of clostridium perfringens that was resistant to bacitracin and produced netb toxin. we identified a bacitracin resistance locus that resembled a bacitracin resistance determinant from enterococcus faecalis. it contained the structural genes bcrabd and a putative reg ... | 2015 | 26282424 |
| decoding the genetic and functional diversity of the dsf quorum-sensing system in stenotrophomonas maltophilia. | stenotrophomonas maltophilia uses the diffusible signal factor (dsf) quorum sensing (qs) system to mediate intra- and inter-specific signaling and regulate virulence-related processes. the components of this system are encoded by the rpf cluster, with genes rpff and rpfc encoding for the dsf synthase rpff and sensor rpfc, respectively. recently, we have shown that there exist two variants of the rpf cluster (rpf-1 and rpf-2), distinguishing two groups of s. maltophilia strains. surprisingly, onl ... | 2015 | 26284046 |
| is the efficacy of biological control against plant diseases likely to be more durable than that of chemical pesticides? | the durability of a control method for plant protection is defined as the persistence of its efficacy in space and time. it depends on (i) the selection pressure exerted by it on populations of plant pathogens and (ii) on the capacity of these pathogens to adapt to the control method. erosion of effectiveness of conventional plant protection methods has been widely studied in the past. for example, apparition of resistance to chemical pesticides in plant pathogens or pests has been extensively d ... | 2015 | 26284088 |
| the xanthomonas effector xopj triggers a conditional hypersensitive response upon treatment of n. benthamiana leaves with salicylic acid. | xopj is a xanthomonas type iii effector protein that promotes bacterial virulence on susceptible pepper plants through the inhibition of the host cell proteasome and a resultant suppression of salicylic acid (sa) - dependent defense responses. we show here that nicotiana benthamiana leaves transiently expressing xopj display hypersensitive response (hr) -like symptoms when exogenously treated with sa. this apparent avirulence function of xopj was further dependent on effector myristoylation as w ... | 2015 | 26284106 |
| assessing the genetic diversity of cu resistance in mine tailings through high-throughput recovery of full-length copa genes. | characterizing the genetic diversity of microbial copper (cu) resistance at the community level remains challenging, mainly due to the polymorphism of the core functional gene copa. in this study, a local blastn method using a copa database built in this study was developed to recover full-length putative copa sequences from an assembled tailings metagenome; these sequences were then screened for potentially functioning copa using conserved metal-binding motifs, inferred by evolutionary trace an ... | 2015 | 26286020 |
| two nucleotide second messengers regulate the production of the vibrio cholerae colonization factor gbpa. | the nucleotide second messengers camp and c-di-gmp allow many bacteria, including the human intestinal pathogen vibrio cholerae, to respond to environmental stimuli with appropriate physiological adaptations. in response to limitation of specific carbohydrates, camp and its receptor crp control the transcription of genes important for nutrient acquisition and utilization; c-di-gmp controls the transition between motile and sessile lifestyles often, but not exclusively, through transcriptional me ... | 2015 | 26286031 |
| disease resistance gene analogs (rgas) in plants. | plants have developed effective mechanisms to recognize and respond to infections caused by pathogens. plant resistance gene analogs (rgas), as resistance (r) gene candidates, have conserved domains and motifs that play specific roles in pathogens' resistance. well-known rgas are nucleotide binding site leucine rich repeats, receptor like kinases, and receptor like proteins. others include pentatricopeptide repeats and apoplastic peroxidases. rgas can be detected using bioinformatics tools based ... | 2015 | 26287177 |
| the multiple dsf-family qs signals are synthesized from carbohydrate and branched-chain amino acids via the fas elongation cycle. | members of the diffusible signal factor (dsf) family are a novel class of quorum sensing (qs) signals in diverse gram-negative bacteria. although previous studies have identified rpff as a key enzyme for the biosynthesis of dsf family signals, many questions in their biosynthesis remain to be addressed. in this study with the phytopathogen xanthomonas campestris pv. campestris (xcc), we show that xcc produces four dsf-family signals (dsf, bdsf, cdsf and idsf) during cell culture, and that idsf i ... | 2015 | 26289160 |
| fleq coordinates flagellum-dependent and -independent motilities in pseudomonas syringae pv. tomato dc3000. | motility plays an essential role in bacterial fitness and colonization in the plant environment, since it favors nutrient acquisition and avoidance of toxic substances, successful competition with other microorganisms, the ability to locate the preferred hosts, access to optimal sites within them, and dispersal in the environment during the course of transmission. in this work, we have observed that the mutation of the flagellar master regulatory gene, fleq, alters bacterial surface motility and ... | 2015 | 26296726 |
| roles of lignin biosynthesis and regulatory genes in plant development. | lignin is an important factor affecting agricultural traits, biofuel production, and the pulping industry. most lignin biosynthesis genes and their regulatory genes are expressed mainly in the vascular bundles of stems and leaves, preferentially in tissues undergoing lignification. other genes are poorly expressed during normal stages of development, but are strongly induced by abiotic or biotic stresses. some are expressed in non-lignifying tissues such as the shoot apical meristem. alterations ... | 2015 | 26297385 |
| beyond phage display: non-traditional applications of the filamentous bacteriophage as a vaccine carrier, therapeutic biologic, and bioconjugation scaffold. | for the past 25 years, phage display technology has been an invaluable tool for studies of protein-protein interactions. however, the inherent biological, biochemical, and biophysical properties of filamentous bacteriophage, as well as the ease of its genetic manipulation, also make it an attractive platform outside the traditional phage display canon. this review will focus on the unique properties of the filamentous bacteriophage and highlight its diverse applications in current research. part ... | 2015 | 26300850 |
| comparative genomic analysis of multiple strains of two unusual plant pathogens: pseudomonas corrugata and pseudomonas mediterranea. | the non-fluorescent pseudomonads, pseudomonas corrugata (pcor) and p. mediterranea (pmed), are closely related species that cause pith necrosis, a disease of tomato that causes severe crop losses. however, they also show strong antagonistic effects against economically important pathogens, demonstrating their potential for utilization as biological control agents. in addition, their metabolic versatility makes them attractive for the production of commercial biomolecules and bioremediation. an e ... | 2015 | 26300874 |
| oligoribonuclease is the primary degradative enzyme for pgpg in pseudomonas aeruginosa that is required for cyclic-di-gmp turnover. | the bacterial second messenger cyclic di-gmp (c-di-gmp) controls biofilm formation and other phenotypes relevant to pathogenesis. cyclic-di-gmp is synthesized by diguanylate cyclases (dgcs). phosphodiesterases (pde-as) end signaling by linearizing c-di-gmp to 5'-phosphoguanylyl-(3',5')-guanosine (pgpg), which is then hydrolyzed to two gmp molecules by yet unidentified enzymes termed pde-bs. we show that pgpg inhibits a pde-a from pseudomonas aeruginosa. in a dual dgc and pde-a reaction, excess p ... | 2015 | 26305945 |
| antibacterial activity of caffeine against plant pathogenic bacteria. | the objective of the present study was to evaluate the antibacterial properties of a plant secondary metabolite - caffeine. caffeine is present in over 100 plant species. antibacterial activity of caffeine was examined against the following plant-pathogenic bacteria: ralstonia solanacearum (rsol), clavibacter michiganesis subsp. sepedonicus (cms), dickeya solani (dsol), pectobacterium atrosepticum (pba), pectobacterium carotovorum subsp. carotovorum (pcc), pseudomonas syringae pv. tomato (pst), ... | 2015 | 26307771 |
| genome-wide identification and validation of reference genes in infected tomato leaves for quantitative rt-pcr analyses. | the gram-negative bacterium xanthomonas campestris pv. vesicatoria (xcv) causes bacterial spot disease of pepper and tomato by direct translocation of type iii effector proteins into the plant cell cytosol. once in the plant cell the effectors interfere with host cell processes and manipulate the plant transcriptome. quantitative rt-pcr (qrt-pcr) is usually the method of choice to analyze transcriptional changes of selected plant genes. reliable results depend, however, on measuring stably expre ... | 2015 | 26313760 |
| an extended cyclic di-gmp network in the predatory bacterium bdellovibrio bacteriovorus. | over the course of the last 3 decades the role of the second messenger cyclic di-gmp (c-di-gmp) as a master regulator of bacterial physiology was determined. although the control over c-di-gmp levels via synthesis and breakdown and the allosteric regulation of c-di-gmp over receptor proteins (effectors) and riboswitches have been extensively studied, relatively few effectors have been identified and most are of unknown functions. the obligate predatory bacterium bdellovibrio bacteriovorus has a ... | 2015 | 26324450 |
| an extended cyclic di-gmp network in the predatory bacterium bdellovibrio bacteriovorus. | over the course of the last 3 decades the role of the second messenger cyclic di-gmp (c-di-gmp) as a master regulator of bacterial physiology was determined. although the control over c-di-gmp levels via synthesis and breakdown and the allosteric regulation of c-di-gmp over receptor proteins (effectors) and riboswitches have been extensively studied, relatively few effectors have been identified and most are of unknown functions. the obligate predatory bacterium bdellovibrio bacteriovorus has a ... | 2015 | 26324450 |
| the spot 42 rna: a regulatory small rna with roles in the central metabolism. | the spot 42 rna is a 109 nucleotide long (in escherichia coli) noncoding small regulatory rna (srna) encoded by the spf (spot fourty-two) gene. spf is found in gamma-proteobacteria and the majority of experimental work on spot 42 rna has been performed using e. coli, and recently aliivibrio salmonicida. in the cell spot 42 rna plays essential roles as a regulator in carbohydrate metabolism and uptake, and its expression is activated by glucose, and inhibited by the camp-crp complex. here we summ ... | 2015 | 26327359 |
| metabolomics reveals differences of metal toxicity in cultures of pseudomonas pseudoalcaligenes kf707 grown on different carbon sources. | co-contamination of metals and organic pollutants is a global problem as metals interfere with the metabolism of complex organics by bacteria. based on a prior observation that metal tolerance was altered by the sole carbon source being used for growth, we sought to understand how metal toxicity specifically affects bacteria using an organic pollutant as their sole carbon source. to this end metabolomics was used to compare cultures of pseudomonas pseudoalcaligenes kf707 grown on either biphenyl ... | 2015 | 26347721 |
| tal effectors and the executor r genes. | transcription activator-like (tal) effectors are bacterial type iii secretion proteins that function as transcription factors in plants during xanthomonas/plant interactions, conditioning either host susceptibility and/or host resistance. three types of tal effector associated resistance (r) genes have been characterized-recessive, dominant non-transcriptional, and dominant tal effector-dependent transcriptional based resistance. here, we discuss the last type of r genes, whose functions are dep ... | 2015 | 26347759 |
| role of host cell-derived amino acids in nutrition of intracellular salmonella enterica. | the facultative intracellular pathogen salmonella enterica resides in a specific membrane-bound compartment termed the salmonella-containing vacuole (scv). despite being segregated from access to metabolites in the host cell cytosol, salmonella is able to efficiently proliferate within the scv. we set out to unravel the nutritional supply of salmonella in the scv with focus on amino acids. we studied the availability of amino acids by the generation of auxotrophic strains for alanine, asparagine ... | 2015 | 26351287 |
| the decoy substrate of a pathogen effector and a pseudokinase specify pathogen-induced modified-self recognition and immunity in plants. | in plants, host response to pathogenic microbes is driven both by microbial perception and detection of modified-self. the xanthomonas campestris effector protein avrac/xopac uridylylates the arabidopsis bik1 kinase to dampen basal resistance and thereby promotes bacterial virulence. here we show that pbl2, a paralog of bik1, is similarly uridylylated by avrac. however, in contrast to bik1, pbl2 uridylylation is specifically required for host recognition of avrac to trigger immunity, but not avr ... | 2015 | 26355215 |
| development of a species-specific pcr assay for three xanthomonas species, causing bulb and flower diseases, based on their genome sequences. | in this study, we developed a species-specific pcr assay for rapid and accurate detection of three xanthomonas species, x. axonopodis pv. poinsettiicola (xap), x. hyacinthi (xh) and x. campestris pv. zantedeschiae (xcz), based on their draft genome sequences. xap, xh and xcz genomes consist of single chromosomes that contain 5,221, 4,395 and 7,986 protein coding genes, respectively. species-specific primers were designed from variable regions of the draft genome sequence data and assessed by a p ... | 2015 | 26361469 |
| dna-binding properties of a cgmp-binding crp homologue that controls development of metabolically dormant cysts of rhodospirillum centenum. | rhodospirillum centenum utilizes 3',5'-cyclic guanosine monophosphate (cgmp) as a messenger to regulate development of desiccation-resistant cysts. in this study, we demonstrated that gcya, gcyb and gcyc, coding for putative subunits of a guanylyl cyclase, increase expression from 8- to 500-fold when cells transition from vegetative to cyst phases of growth. this induction did not occur in a strain that is defective in cgmp synthesis or in a strain that contains a deletion of cgra that codes for ... | 2015 | 26362215 |
| type iii secretion systems: the bacterial flagellum and the injectisome. | the flagellum and the injectisome are two of the most complex and fascinating bacterial nanomachines. at their core, they share a type iii secretion system (t3ss), a transmembrane export complex that forms the extracellular appendages, the flagellar filament and the injectisome needle. recent advances, combining structural biology, cryo-electron tomography, molecular genetics, in vivo imaging, bioinformatics and biophysics, have greatly increased our understanding of the t3ss, especially the str ... | 2015 | 26370933 |
| maize homologs of hydroxycinnamoyltransferase, a key enzyme in lignin biosynthesis, bind the nucleotide binding leucine-rich repeat rp1 proteins to modulate the defense response. | in plants, most disease resistance genes encode nucleotide binding leu-rich repeat (nlr) proteins that trigger a rapid localized cell death called a hypersensitive response (hr) upon pathogen recognition. the maize (zea mays) nlr protein rp1-d21 derives from an intragenic recombination between two nlrs, rp1-d and rp1-dp2, and confers an autoactive hr in the absence of pathogen infection. from a previous quantitative trait loci and genome-wide association study, we identified a single-nucleotide ... | 2015 | 26373661 |
| insights into substrate specificity of nlpc/p60 cell wall hydrolases containing bacterial sh3 domains. | bacterial sh3 (sh3b) domains are commonly fused with papain-like nlp/p60 cell wall hydrolase domains. to understand how the modular architecture of sh3b and nlpc/p60 affects the activity of the catalytic domain, three putative nlpc/p60 cell wall hydrolases were biochemically and structurally characterized. these enzymes all have γ-d-glu-a2pm (a2pm is diaminopimelic acid) cysteine amidase (or dl-endopeptidase) activities but with different substrate specificities. one enzyme is a cell wall lysin ... | 2015 | 26374125 |
| new insights on molecular regulation of biofilm formation in plant-associated bacteria. | biofilms are complex bacterial assemblages with a defined three-dimensional architecture, attached to solid surfaces, and surrounded by a self-produced matrix generally composed of exopolysaccharides, proteins, lipids and extracellular dna. biofilm formation has evolved as an adaptive strategy of bacteria to cope with harsh environmental conditions as well as to establish antagonistic or beneficial interactions with their host. plant-associated bacteria attach and form biofilms on different tiss ... | 2016 | 26377849 |
| controlling weeds with fungi, bacteria and viruses: a review. | weeds are a nuisance in a variety of land uses. the increasing prevalence of both herbicide resistant weeds and bans on cosmetic pesticide use has created a strong impetus to develop novel strategies for controlling weeds. the application of bacteria, fungi and viruses to achieving this goal has received increasingly great attention over the last three decades. proposed benefits to this strategy include reduced environmental impact, increased target specificity, reduced development costs compare ... | 2015 | 26379687 |
| genome sequences of the race 1 and race 4 xanthomonas campestris pv. campestris strains cfbp 1869 and cfbp 5817. | xanthomonas campestris pv. campestris is the causal agent of black rot on brassicaceae. the draft genome sequences of strains cfbp 1869 and cfbp 5817 have been determined and are the first ones corresponding to race 1 and race 4 strains, which have a predominant agronomic and economic impact on cabbage cultures worldwide. | 2015 | 26383661 |
| o antigen modulates insect vector acquisition of the bacterial plant pathogen xylella fastidiosa. | hemipteran insect vectors transmit the majority of plant pathogens. acquisition of pathogenic bacteria by these piercing/sucking insects requires intimate associations between the bacterial cells and insect surfaces. lipopolysaccharide (lps) is the predominant macromolecule displayed on the cell surface of gram-negative bacteria and thus mediates bacterial interactions with the environment and potential hosts. we hypothesized that bacterial cell surface properties mediated by lps would be import ... | 2015 | 26386068 |
| iron is a signal for stenotrophomonas maltophilia biofilm formation, oxidative stress response, omps expression, and virulence. | stenotrophomonas maltophilia is an emerging nosocomial pathogen. in many bacteria iron availability regulates, through the fur system, not only iron homeostasis but also virulence. the aim of this work was to assess the role of iron on s. maltophilia biofilm formation, eps production, oxidative stress response, omps regulation, quorum sensing (qs), and virulence. studies were done on k279a and its isogenic fur mutant f60 cultured in the presence or absence of dipyridyl. this is the first report ... | 2015 | 26388863 |
| regulatory proteolysis in arabidopsis-pathogen interactions. | approximately two and a half percent of protein coding genes in arabidopsis encode enzymes with known or putative proteolytic activity. proteases possess not only common housekeeping functions by recycling nonfunctional proteins. by irreversibly cleaving other proteins, they regulate crucial developmental processes and control responses to environmental changes. regulatory proteolysis is also indispensable in interactions between plants and their microbial pathogens. proteolytic cleavage is simu ... | 2015 | 26404238 |
| designing potent antimicrobial peptides by disulphide linked dimerization and n-terminal lipidation to increase antimicrobial activity and membrane perturbation: structural insights into lipopolysaccharide binding. | the remarkable rise in multi-drug resistant gram-negative bacterial pathogens is a major concern to the well being of humans as well as susceptible plants. in recent years, diseases associated with inflammation and septicemia have already become a global health issue. therefore, there is a rising demand for the development of novel "super" antibiotics. in this context, antimicrobial peptides offer an attractive, alternate therapeutic solution to conventional antibiotics. | 2016 | 26407061 |
| ex-ante economic impact assessment of genetically modified banana resistant to xanthomonas wilt in the great lakes region of africa. | credible empirical evidence is scanty on the social implications of genetically modified (gm) crops in africa, especially on vegetatively propagated crops. little is known about the future success of introducing gm technologies into staple crops such as bananas, which are widely produced and consumed in the great lakes region of africa (gla). gm banana has a potential to control the destructive banana xanthomonas wilt disease. | 2015 | 26414379 |
| mini-tn7 vectors for stable expression of diguanylate cyclase pled* in gram-negative bacteria. | the cyclic diguanylate (c-di-gmp) is currently considered an ubiquitous second messenger in bacteria that influences a wide range of cellular processes. one of the methodological approaches to unravel c-di-gmp regulatory networks involves raising the c-di-gmp intracellular levels, e.g. by expressing a diguanylate cyclase (dgc), to provoke phenotypic changes. | 2015 | 26415513 |
| in vitro control of plant pathogenic xanthomonas spp. using poncirus trifoliata rafin. | the secondary metabolites such as essential oil and pure compounds (limonin and imperatorin) from poncirus trifoliata rafin were tested for in vitro control of phytopathogenic bacteria of xanthomonas spp. in vitro studies showed that the oil had inhibitory effect on xanthomonas campestris pv. compestris kc94-17-xcc, xanthomonas campestris pv. vesicatoria yk93-4-xcv, xanthomonas oryzae pv. oryzae kx019-xco and xanthomonas sp. sk12 with their inhibition zones and minimum inhibitory concentration ( ... | 2014 | 26417325 |
| a novel and widespread class of ketosynthase is responsible for the head-to-head condensation of two acyl moieties in bacterial pyrone biosynthesis. | the biosynthesis of photopyrones, novel quorum sensing signals in photorhabdus, has been studied by heterologous expression of the photopyrone synthase ppys catalyzing the head-to-head condensation of two acyl moieties. the biochemical mechanism of pyrone formation has been investigated by amino acid exchange and bioinformatic analysis. additionally, the evolutionary origin of ppys has been studied by phylogenetic analyses also revealing homologous enzymes in pseudomonas sp. gm30 responsible for ... | 2015 | 26425196 |
| rapid, specific, simple, in-field detection of xanthomonas campestris pathovar musacearum by loop-mediated isothermal amplification. | to develop and evaluate a loop-mediated isothermal amplification (lamp) assay for xanthomonas campestris pathovar musacearum (xcm), the causal agent of banana xanthomonas wilt, a major disease of banana in africa. | 2015 | 26425811 |
| a taqman-based multiplex qpcr assay and dna extraction method for phylotype iib sequevars 1&2 (select agent) strains of ralstonia solanacearum. | ralstonia solanacearum race 3 biovar 2 strains belonging to phylotype iib, sequevars 1 and 2 (iib-1&2) cause brown rot of potato in temperate climates, and are quarantined pathogens in canada and europe. since these strains are not established in the u.s. and because of their potential risk to the potato industry, the u.s. government has listed them as select agents. cultivated geraniums are also a host and have the potential to spread the pathogen through trade, and its extracts strongly inhibi ... | 2015 | 26426354 |
| pbl13 is a serine/threonine protein kinase that negatively regulates arabidopsis immune responses. | receptor-like cytoplasmic kinases (rlcks) are a subset of plant receptor-like kinases lacking both extracellular and transmembrane domains. some of the 46 members in the arabidopsis (arabidopsis thaliana) rlck subfamily vii have been linked to plant innate immunity; however, most remain uncharacterized. thus, multiple subfamily vii members are expected to be involved in plant immune signaling. here, we investigate the role of avrpphb susceptible1-like13 (pbl13), a subfamily vii rlck with unique ... | 2015 | 26432875 |
| backbone chemical shift assignments for xanthomonas campestris peroxiredoxin q in the reduced and oxidized states: a dramatic change in backbone dynamics. | peroxiredoxins (prx) are ubiquitous enzymes that reduce peroxides as part of antioxidant defenses and redox signaling. while prx catalytic activity and sensitivity to hyperoxidative inactivation depend on their dynamic properties, there are few examples where their dynamics has been characterized by nmr spectroscopy. here, we provide a foundation for studies of the solution properties of peroxiredoxin q from the plant pathogen xanthomonas campestris (xcprxq) by assigning the observable (1)h(n), ... | 2016 | 26438558 |
| priming of pathogenesis related-proteins and enzymes related to oxidative stress by plant growth promoting rhizobacteria on rice plants upon abiotic and biotic stress challenge. | two plant growth promoting rhizobacteria (pgpr) were tested to evaluate their capacity to prime rice seedlings against stress challenge (salt and xanthomonas campestris infection). as is accepted that plants respond to biotic and abiotic stresses by generation of reactive oxygen species (ros), enzyme activities related to oxidative stress (ascorbate peroxidase (apx, ec 1.11.1.11), guaiacol peroxidase (gpx, ec 1.11.1.7), glutathione reductase (gr, ec 1.6.4.2) and superoxide dismutase (sod, ec 1.1 ... | 2015 | 26439659 |
| biopolymers from lactic acid bacteria. novel applications in foods and beverages. | lactic acid bacteria (lab) are microorganisms widely used in the fermented food industry worldwide. certain lab are able to produce exopolysaccharides (eps) either attached to the cell wall (capsular eps) or released to the extracellular environment (eps). according to their composition, lab may synthesize heteropolysaccharides or homopolysaccharides. a wide diversity of eps are produced by lab concerning their monomer composition, molecular mass, and structure. although eps-producing lab strain ... | 2015 | 26441845 |
| systemic responses in a tolerant olive (olea europaea l.) cultivar upon root colonization by the vascular pathogen verticillium dahliae. | verticillium wilt of olive (vwo) is caused by the vascular pathogen verticillium dahliae. one of the best vwo management measures is the use of tolerant cultivars; however, our knowledge on vwo tolerance/resistance genetics is very limited. a transcriptomic analysis was conducted to (i) identify systemic defense responses induced/repressed in aerial tissues of the tolerant cultivar frantoio upon root colonization by v. dahliae, and (ii) determine the expression pattern of selected defense genes ... | 2015 | 26441865 |
| a novel workflow correlating rna-seq data to phythophthora infestans resistance levels in wild solanum species and potato clones. | comparative transcriptomics between species can provide valuable understanding of plant-pathogen interactions. here, we focus on wild solanum species and potato clones with varying degree of resistance against phytophthora infestans, which causes the devastating late blight disease in potato. the transcriptomes of three wild solanum species native to southern sweden, solanum dulcamara, solanum nigrum, and solanum physalifolium were compared to three potato clones, desiree (cv.), sw93-1015 and sa ... | 2015 | 26442032 |
| selection and validation of potato candidate genes for maturity corrected resistance to phytophthora infestans based on differential expression combined with snp association and linkage mapping. | late blight of potato (solanum tuberosum l.) caused by the oomycete phytophthora infestans (mont.) de bary, is one of the most important bottlenecks of potato production worldwide. cultivars with high levels of durable, race unspecific, quantitative resistance are part of a solution to this problem. however, breeding for quantitative resistance is hampered by the correlation between resistance and late plant maturity, which is an undesirable agricultural attribute. the objectives of our research ... | 2015 | 26442110 |
| edwardsiella tarda esce (orf13 protein) is a type iii secretion system-secreted protein that is required for the injection of effectors, secretion of translocators, and pathogenesis in fish. | the type iii secretion system (t3ss) of edwardsiella tarda is crucial for its intracellular survival and pathogenesis in fish. the orf13 gene (esce) of e. tarda is located 84 nucleotides (nt) upstream of esrc in the t3ss gene cluster. we found that esce is secreted and translocated in a t3ss-dependent manner and that amino acids 2 to 15 in the n terminus were required for a completely functional t3ss in e. tarda. deletion of esce abolished the secretion of t3ss translocators, as well as the secr ... | 2015 | 26459509 |
| edwardsiella tarda esce (orf13 protein) is a type iii secretion system-secreted protein that is required for the injection of effectors, secretion of translocators, and pathogenesis in fish. | the type iii secretion system (t3ss) of edwardsiella tarda is crucial for its intracellular survival and pathogenesis in fish. the orf13 gene (esce) of e. tarda is located 84 nucleotides (nt) upstream of esrc in the t3ss gene cluster. we found that esce is secreted and translocated in a t3ss-dependent manner and that amino acids 2 to 15 in the n terminus were required for a completely functional t3ss in e. tarda. deletion of esce abolished the secretion of t3ss translocators, as well as the secr ... | 2015 | 26459509 |
| brevibacillus laterosporus, a pathogen of invertebrates and a broad-spectrum antimicrobial species. | brevibacillus laterosporus, a bacterium characterized by the production of a unique canoe-shaped lamellar body attached to one side of the spore, is a natural inhabitant of water, soil and insects. its biopesticidal potential has been reported against insects in different orders including coleoptera, lepidoptera, diptera and against nematodes and mollusks. in addition to its pathogenicity against invertebrates, different b. laterosporus strains show a broad-spectrum antimicrobial activity includ ... | 2013 | 26462431 |
| phytopath: an integrative resource for plant pathogen genomics. | phytopath (www.phytopathdb.org) is a resource for genomic and phenotypic data from plant pathogen species, that integrates phenotypic data for genes from phi-base, an expertly curated catalog of genes with experimentally verified pathogenicity, with the ensembl tools for data visualization and analysis. the resource is focused on fungi, protists (oomycetes) and bacterial plant pathogens that have genomes that have been sequenced and annotated. genes with associated phi-base data can be easily id ... | 2015 | 26476449 |
| phytopath: an integrative resource for plant pathogen genomics. | phytopath (www.phytopathdb.org) is a resource for genomic and phenotypic data from plant pathogen species, that integrates phenotypic data for genes from phi-base, an expertly curated catalog of genes with experimentally verified pathogenicity, with the ensembl tools for data visualization and analysis. the resource is focused on fungi, protists (oomycetes) and bacterial plant pathogens that have genomes that have been sequenced and annotated. genes with associated phi-base data can be easily id ... | 2015 | 26476449 |
| ethylene: traffic controller on hormonal crossroads to defense. | ethylene (et) is an important hormone in plant responses to microbial pathogens and herbivorous insects, and in the interaction of plants with beneficial microbes and insects. early et signaling events during these biotic interactions involve activities of mitogen-activated protein kinases and ethylene response factor transcription factors. rather than being the principal regulator, et often modulates defense signaling pathways, including those regulated by jasmonic acid and salicylic acid. horm ... | 2015 | 26482888 |
| fleq dna binding consensus sequence revealed by studies of fleq-dependent regulation of biofilm gene expression in pseudomonas aeruginosa. | the transcription factor fleq from pseudomonas aeruginosa derepresses expression of genes involved in biofilm formation when intracellular levels of the second messenger cyclic diguanosine monophosphate (c-di-gmp) are high. fleq also activates transcription of flagellar genes, and the expression of these genes is highest at low intracellular c-di-gmp. fleq thus plays a central role in mediating the transition between planktonic and biofilm lifestyles of p. aeruginosa. previous work showed that f ... | 2015 | 26483521 |
| fleq dna binding consensus sequence revealed by studies of fleq-dependent regulation of biofilm gene expression in pseudomonas aeruginosa. | the transcription factor fleq from pseudomonas aeruginosa derepresses expression of genes involved in biofilm formation when intracellular levels of the second messenger cyclic diguanosine monophosphate (c-di-gmp) are high. fleq also activates transcription of flagellar genes, and the expression of these genes is highest at low intracellular c-di-gmp. fleq thus plays a central role in mediating the transition between planktonic and biofilm lifestyles of p. aeruginosa. previous work showed that f ... | 2015 | 26483521 |
| present and future medical applications of microbial exopolysaccharides. | microbial exopolysaccharides (eps) have found outstanding medical applications since the mid-20th century, with the first clinical trials on dextran solutions as plasma expanders. other eps entered medicine firstly as conventional pharmaceutical excipients (e.g., xanthan - as suspension stabilizer, or pullulan - in capsules and oral care products). polysaccharides, initially obtained from plant or animal sources, became easily available for a wide range of applications, especially when they were ... | 2015 | 26483763 |
| ubiquity, diversity and physiological characteristics of geodermatophilaceae in shapotou national desert ecological reserve. | the goal of this study was to gain insight into the diversity of culturable actinobacteria in desert soil crusts and to determine the physiological characteristics of the predominant actinobacterial group in these crusts. culture-dependent method was employed to obtain actinobacterial strains from desert soil samples collected from shapotou national desert ecological reserve (nder) located in tengger desert, china. a total of 376 actinobacterial strains were isolated and 16s rrna gene sequences ... | 2015 | 26483778 |
| sigmol: repertoire of quorum sensing signaling molecules in prokaryotes. | quorum sensing is a widespread phenomenon in prokaryotes that helps them to communicate among themselves and with eukaryotes. it is driven through quorum sensing signaling molecules (qssms) in a density dependent manner that assists in numerous biological functions like biofilm formation, virulence factors secretion, swarming motility, bioluminescence, etc. despite immense implications, dedicated resources of qssms are lacking. therefore, we have developed sigmol (http://bioinfo.imtech.res.in/ma ... | 2015 | 26490957 |
| sigmol: repertoire of quorum sensing signaling molecules in prokaryotes. | quorum sensing is a widespread phenomenon in prokaryotes that helps them to communicate among themselves and with eukaryotes. it is driven through quorum sensing signaling molecules (qssms) in a density dependent manner that assists in numerous biological functions like biofilm formation, virulence factors secretion, swarming motility, bioluminescence, etc. despite immense implications, dedicated resources of qssms are lacking. therefore, we have developed sigmol (http://bioinfo.imtech.res.in/ma ... | 2015 | 26490957 |
| evidence for a functional o-linked n-acetylglucosamine (o-glcnac) system in the thermophilic bacterium thermobaculum terrenum. | post-translational modification of proteins is a ubiquitous mechanism of signal transduction in all kingdoms of life. one such modification is addition of o-linked n-acetylglucosamine to serine or threonine residues, known as o-glcnacylation. this unusual type of glycosylation is thought to be restricted to nucleocytoplasmic proteins of eukaryotes and is mediated by a pair of o-glcnac-transferase and o-glcnac hydrolase enzymes operating on a large number of substrate proteins. protein o-glcnacyl ... | 2015 | 26491011 |
| complete sequence and detailed analysis of the first indigenous plasmid from xanthomonas oryzae pv. oryzicola. | bacterial plasmids have a major impact on metabolic function and adaptation of their hosts. an indigenous plasmid was identified in a chinese isolate (gx01) of the invasive phytopathogen xanthomonas oryzae pv. oryzicola (xoc), the causal agent of rice bacterial leaf streak (bls). to elucidate the biological functions of the plasmid, we have sequenced and comprehensively annotated the plasmid. | 2015 | 26498126 |
| biocide effects of volatile organic compounds produced by potential biocontrol rhizobacteria on sclerotinia sclerotiorum. | six rhizobacteria isolated from common bean and able to protect bean plants from the common bacterial blight (cbb) causal agent, were in vitro evaluated for their potential antifungal effects toward different plant pathogenic fungi, mostly soil-borne. by dual culture assays, the above bacteria resulted producing diffusible and volatile metabolites which inhibited the growth of the majority of the pathogens under study. in particular, the latter substances highly affected the mycelium growth of s ... | 2015 | 26500617 |
| biomedical biopolymers, their origin and evolution in biomedical sciences: a systematic review. | biopolymers provide a plethora of applications in the pharmaceutical and medical applications. a material that can be used for biomedical applications like wound healing, drug delivery and tissue engineering should possess certain properties like biocompatibility, biodegradation to non-toxic products, low antigenicity, high bio-activity, processability to complicated shapes with appropriate porosity, ability to support cell growth and proliferation and appropriate mechanical properties, as well ... | 2015 | 26501034 |
| the inheritance of resistance to bacterial leaf spot of lettuce caused by xanthomonas campestris pv. vitians in three lettuce cultivars. | lettuce yields can be reduced by the disease bacterial leaf spot (bls) caused by the pathogen xanthomonas campestris pv. vitians (xcv) and host resistance is the most feasible method to reduce disease losses. the cultivars la brillante, pavane and little gem express an incompatible host-pathogen interaction as a hypersensitive response (hr) to california strains of xcv resulting in resistance. little was known about the inheritance of resistance; however, resistance to other lettuce pathogens is ... | 2014 | 26504558 |
| construction of citrus gene coexpression networks from microarray data using random matrix theory. | after the sequencing of citrus genomes, gene function annotation is becoming a new challenge. gene coexpression analysis can be employed for function annotation using publicly available microarray data sets. in this study, 230 sweet orange (citrus sinensis) microarrays were used to construct seven coexpression networks, including one condition-independent and six condition-dependent (citrus canker, huanglongbing, leaves, flavedo, albedo, and flesh) networks. in total, these networks contain 37 6 ... | 2015 | 26504573 |
| systematic identification of cyclic-di-gmp binding proteins in vibrio cholerae reveals a novel class of cyclic-di-gmp-binding atpases associated with type ii secretion systems. | cyclic-di-gmp (c-di-gmp) is a ubiquitous bacterial signaling molecule that regulates a variety of complex processes through a diverse set of c-di-gmp receptor proteins. we have utilized a systematic approach to identify c-di-gmp receptors from the pathogen vibrio cholerae using the differential radial capillary action of ligand assay (dracala). the dracala screen identified a majority of known c-di-gmp binding proteins in v. cholerae and revealed a novel c-di-gmp binding protein, mshe (vc0405), ... | 2015 | 26506097 |