Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| [clinical features of protracted bacterial bronchitis in children]. | to analyze the clinical characteristics of protracted bacterial bronchitis (pbb) in children. | 2015 | 26758117 |
| laboratory characterization of invasive haemophilus influenzae isolates from nunavut, canada, 2000-2012. | with invasive haemophilus influenzae serotype b (hib) disease controlled by vaccination with conjugate hib vaccines, there is concern that invasive disease due to non-serotype b strains may emerge. | 2016 | 26765260 |
| dna aptamers for the detection of haemophilus influenzae type b by cell selex. | haemophilus influenzae type b (hib) causes acute bacterial meningitis (abm) in children, with a mortality rate of about 3-6 % of the affected patients. abm can lead to death during a period of hours to several days and, hence, rapid and early detection of the infection is crucial. aptamers, the short single-stranded dna or rna with high affinity to target molecules, are selected by a high-flux screening technique known as in vitro screening and systematic evolution of ligands by exponential enri ... | 2016 | 26768582 |
| [bacterial pathogenic characteristics of respiratory tract infection in children in suzhou, china: an analysis of 14,994 cases]. | to investigate the bacterial pathogenic characteristics of respiratory tract infection in children. | 2016 | 26781412 |
| copd is characterized by increased detection of haemophilus influenzae, streptococcus pneumoniae and a deficiency of bacillus species. | chronic obstructive pulmonary disease (copd) is characterized by progressive airflow limitation and inflammation. airway bacterial colonization is increased in copd; however, the role of potentially pathogenic and non-pathogenic bacteria in the pathogenesis of disease is unclear. this study characterized the presence of bacteria in a well-characterized cohort of adults with copd and healthy controls. | 2016 | 26781464 |
| otitis media: a review, with a focus on alternative treatments. | otitis media (om) is the accumulation of fluids in the middle ear, with or without symptoms of inflammation. the infection is caused by dysfunction or obstruction of the eustachian tube and is most commonly diagnosed in children under the age of two. the microbiology of om differs, with streptococcus pneumoniae, non-typeable haemophilus influenzae and moraxella catarrhalis the most commonly isolated pathogens. the emergence of penicillin-resistant strep. pneumoniae, β-lactamase-producing strains ... | 2009 | 26783131 |
| correction: detection of streptococcus pneumoniae and haemophilus influenzae type b by real-time pcr from dried blood spot samples among children with pneumonia: a useful approach for developing countries. | 2016 | 26785260 | |
| [real-time pcr detection of neisseria meningitidis, haemophilus influenzae, and streptococcus pneumoniae dna in clinical specimens]. | the study aim was to implement a molecular real-time polymerase chain reaction (pcr) assay recommended by the cdc (centers for disease control and prevention) for the detection of neisseria meningitidis, haemophilus influenzae, and streptococcus pneumoniae in clinical (culture negative) specimens from patients with suspected invasive bacterial disease. clinical specimens are referred to the national reference laboratory (nrl) for meningococcal infections, unit for airborne bacterial infections, ... | 2015 | 26795226 |
| a novel mechanism for the biogenesis of outer membrane vesicles in gram-negative bacteria. | bacterial outer membrane vesicles (omvs) have important biological roles in pathogenesis and intercellular interactions, but a general mechanism of omv formation is lacking. here we show that the vacj/yrb abc (atp-binding cassette) transport system, a proposed phospholipid transporter, is involved in omv formation. deletion or repression of vacj/yrb increases omv production in two distantly related gram-negative bacteria, haemophilus influenzae and vibrio cholerae. lipidome analyses demonstrate ... | 2016 | 26806181 |
| molecular epidemiology of ampicillin-resistant haemophilus influenzae causing acute otitis media in japanese infants and young children. | nontypeable haemophilus influenzae is a particularly important cause of acute otitis media (aom). there is a high prevalence of β-lactamase-nonproducing ampicillin-resistant (blnar) strains in japanese children, which is associated with recurrent aom and prolonged treatment. the aim of this study was to investigate the antimicrobial susceptibility profile, mechanisms of ampicillin resistance and molecular epidemiology of ampicillin resistance in h. influenzae strains causing aom in japanese chil ... | 2016 | 26808724 |
| process development of a new haemophilus influenzae type b conjugate vaccine and the use of mathematical modeling to identify process optimization possibilities. | vaccination is one of the most successful public health interventions being a cost-effective tool in preventing deaths among young children. the earliest vaccines were developed following empirical methods, creating vaccines by trial and error. new process development tools, for example mathematical modeling, as well as new regulatory initiatives requiring better understanding of both the product and the process are being applied to well-characterized biopharmaceuticals (for example recombinant ... | 2016 | 26821825 |
| development of real-time pcr methods for the detection of bacterial meningitis pathogens without dna extraction. | neisseria meningitidis (nm), haemophilus influenzae (hi), and streptococcus pneumoniae (sp) are the lead causes of bacterial meningitis. detection of these pathogens from clinical specimens using traditional real-time pcr (rt-pcr) requires dna extraction to remove the pcr inhibitors prior to testing, which is time consuming and labor intensive. in this study, five species-specific (nm-sodc and -ctra, hi-hpd#1 and -hpd#3 and sp-lyta) and six serogroup-specific rt-pcr tests (a, b, c, w, x, y) targ ... | 2016 | 26829233 |
| a pilot study of quantitative loop-mediated isothermal amplification-guided target therapies for hospital-acquired pneumonia. | it is important to achieve the definitive pathogen identification in hospital-acquired pneumonia (hap), but the traditional culture results always delay the target antibiotic therapy. we assessed the method called quantitative loop-mediated isothermal amplification (qlamp) as a new implement for steering of the antibiotic decision-making in hap. | 2016 | 26830989 |
| diagnosis of upper and lower respiratory tract bacterial infections with the use of multiplex pcr assays. | the investigation of respiratory infections by molecular techniques provides important information about the epidemiology of respiratory disease, especially during the post-vaccination era. the objective of the present study was the detection of bacterial pathogens directly in clinical samples from patients with upper and lower respiratory tract infections using multiplex polymerase chain reaction (pcr) assays developed in our laboratory. clinical samples taken over a three-year period (2007-200 ... | 2013 | 26835676 |
| factors associated with parental refusal of routine vaccination in the czech republic. | routine vaccination is one of the most important preventive methods which is responsible for the decreasing trend of morbidity and mortality of vaccine preventable infectious diseases, their complications and sequelae. the impact of vaccination on declining trend of these diseases is well known and confirmed by a large number of epidemiological studies. in the czech republic, there is high vaccination coverage in regards to most vaccine preventable diseases. however, during the last decade propo ... | 2015 | 26841145 |
| non-typeable haemophilus influenzae purulent pericarditis in a child with cystic fibrosis. | early airway colonization and infection with haemophilus influenzae in children with cystic fibrosis (cf) is common. although the pathogenicity of non-typeable h. influenzae (nthi) in patients with cf is controversial, this organism can cause both upper and lower respiratory tract infections. extra-pulmonary disease, however, is rare. purulent pericarditis is a suppurative complication of bacterial infection of the pericardial space that can arise as a result of direct extension from an adjacent ... | 2016 | 26842501 |
| resolvin d1 dampens pulmonary inflammation and promotes clearance of nontypeable haemophilus influenzae. | nontypeable haemophilus influenzae (nthi) is a gram-negative, opportunistic pathogen that frequently causes ear infections, bronchitis, pneumonia, and exacerbations in patients with underlying inflammatory diseases, such as chronic obstructive pulmonary disease. in mice, nthi is rapidly cleared, but a strong inflammatory response persists, underscoring the concept that nthi induces dysregulation of normal inflammatory responses and causes a failure to resolve. lipid-derived specialized proresolv ... | 2016 | 26843331 |
| invasive haemophilus influenzae serotype f case reports in mazovia province, poland. | after successful introduction of anti-haemophilus influenzae (hi) serotype b vaccination program in poland, invasive non-b or nontypeable h. influenzae infections have been reported more frequently alike in other countries all over the world. in this paper, we report 2 cases of h. influenzae serotype f (hif) meningitis with severe clinical presentations which are rarely seen in previously healthy children.the first case is a 6-year-old girl who was admitted to pediatric ward with signs of mening ... | 2016 | 26844500 |
| male non-gonococcal urethritis: from microbiological etiologies to demographic and clinical features. | to detect microorganisms responsible for male acute urethritis and to define the microbiology of non-gonococcal urethritis. | 2016 | 26845624 |
| streptococcus pneumoniae and haemophilus species colonization in health care workers: the launch of invasive infections? | streptococcus pneumoniae and haemophilus influenzae are important human pathogens. the risk of airborne and droplet-transmitted respiratory tract infections in healthcare workers (hcw) is substantial. the aim of this study was to determine the extent of oropharyngeal colonization with s. pneumoniae and haemophilus spp. their antibiogram and risk factors of colonization in hcw at a tertiary care center, western nepal. | 2016 | 26847639 |
| the role of solithromycin in the management of bacterial community-acquired pneumonia. | the fluoroketolide solithromycin is 2-fold more potent in vitro than telithromycin against pneumococci (including macrolide-resistant strains) and haemophilus influenzae and very active on pathogens causing atypical pneumonia. in contrast, it is a 30-fold less potent inhibitor of nicotinic receptors incriminated in telithromycin toxicity. in phase ii/iii trials, oral solithromycin once-daily (800 mg on day 1; 400 mg on days 2-5) proved effective and safe when compared to respiratory fluoroquinol ... | 2016 | 26848612 |
| effect of haemophilus influenzae type b vaccination without a booster dose on invasive h influenzae type b disease, nasopharyngeal carriage, and population immunity in kilifi, kenya: a 15-year regional surveillance study. | haemophilus influenzae type b (hib) conjugate vaccine, delivered as a three-dose series without a booster, was introduced into the childhood vaccination programme in kenya in 2001. the duration of protection and need for a booster dose are unknown. we aimed to assess vaccine effectiveness, the impact of the vaccine on nasopharyngeal carriage, and population immunity after introduction of conjugate hib vaccine in infancy without a booster dose in kenya. | 2016 | 26853149 |
| rationale and prospects of targeting bacterial two-component systems for antibacterial treatment of cystic fibrosis patients. | bacterial respiratory infections are the main reason of morbidity and mortality among cystic fibrosis (cf) patients. in early childhood, the respiratory infections are due to staphylococcus aureus and haemophilus influenzae. in older cf patients, pathogenic gram-negative bacteria like achromobacter xylosoxidans, burkholderia cepacia complex and especially pseudomonas aeruginosa are more frequently seen. p. aeruginosa is a turning point in the respiratory disease in cf and its predominance increa ... | 2016 | 26853320 |
| the impact of exogenous factors on respiratory pathogen-induced innate alveolar macrophage responses in copd. | alveolar macrophages in chronic obstructive pulmonary disease (copd) have fundamentally impaired innate immune responses to toll-like receptor (tlr) ligands of nontypeable haemophilus influenzae (nthi). however, whether dysfunctional inflammatory responses in copd extend to macrophage interactions with intact respiratory pathogens beyond nthi has not been explored. furthermore, the influences of exogenous factors, including active smoking and medications, on pathogen-induced innate immune respon ... | 2016 | 26853802 |
| microbial colonization and lung function in adolescents with cystic fibrosis. | with intensified antibiotic therapy and longer survival, patients with cystic fibrosis (cf) are colonized with a more complex pattern of bacteria and fungi. however, the clinical relevance of these emerging pathogens for lung function remains poorly defined. the aim of this study was to assess the association of bacterial and fungal colonization patterns with lung function in adolescent patients with cf. microbial colonization patterns and lung function parameters were assessed in 770 adolescent ... | 2016 | 26856310 |
| intercellular adhesion molecule 1 serves as a primary cognate receptor for the type iv pilus of nontypeable haemophilus influenzae. | nontypeable haemophilus influenzae (nthi) utilizes the type iv pilus (tfp) to adhere to respiratory tract epithelial cells thus colonizing its human host; however, the host cell receptor to which this adhesive protein binds is unknown. from a panel of receptors engaged by tfp expressed by other bacterial species, we showed that the majority subunit of nthi tfp, pila, bound to intercellular adhesion molecule 1 (icam1) and that this interaction was both specific and of high affinity. further, tfp- ... | 2016 | 26857242 |
| supersilac quantitative proteome profiling of murine middle ear epithelial cell remodeling with nthi. | chronic otitis media with effusion (come) develops after sustained inflammation and is characterized by secretory middle ear epithelial metaplasia and effusion, most frequently mucoid. non-typeable haemophilus influenzae (nthi), the most common acute otitis media (om) pathogen, is postulated to promote middle ear epithelial remodeling in the progression of om from acute to chronic. the goals of this study were to examine histopathological and quantitative proteomic epithelial effects of nthi cha ... | 2016 | 26859300 |
| synthesis and antibacterial evaluation of novel 4″-glycyl linked quinolyl-azithromycins with potent activity against macrolide-resistant pathogens. | a new azithromycin-based series of antibacterial macrolones is reported, which features the use of a 4″-ester linked glycin for tethering the quinolone side chain to the macrolide scaffold. among the analogs prepared, compounds 9e and 22f with a quinolon-6-yl moiety were found to have potent and well-balanced activity against clinically important respiratory tract pathogens, including erythromycin-susceptible and mlsb resistant strains of streptococcus pneumoniae, streptococcus pyogenes, and hae ... | 2016 | 26860929 |
| clinical characteristics of the patients with bacteremia due to moraxella catarrhalis in children: a case-control study. | invasive diseases due to moraxella catarrhalis are rare in children, even in immunocompromised hosts. therefore, data regarding clinical characteristics and risk factors of such patients are limited. the aim of this study is to compare the clinical characteristics of patients with bacteremia due to moraxella catarrhalis against those with bacteremia due to streptococcus pneumoniae and haemophilus influenzae. | 2015 | 26861621 |
| safety of a crm197-conjugated haemophilus influenzae type b vaccine in korean children. | haemophilus influenzae type b (hib) is a major cause of meningitis and pneumonia with high morbidity and mortality rates in young children. the introduction of effective and well-tolerated conjugate hib vaccines, has nearly eradicated this disease in many countries. we investigated the safety of the hib prp-crm197 vaccine in a multi-center post-marketing surveillance (pms) study. korean children (n = 764) aged 1-33 months were enrolled when receiving a routine primary immunization or a booster v ... | 2015 | 26867395 |
| immunogenicity and safety of quinvaxem® (diphtheria, tetanus, whole-cell pertussis, hepatitis b and haemophilus influenzae type b vaccine) given to vietnamese infants at 2 to 4 months of age. | vietnam plans to replace the routine childhood diphtheria, pertussis and tetanus combination (dpt) vaccine with a pentavalent vaccine. the present study was performed to assess the immunogenicity and safety of the combined diphtheria, tetanus, whole-cell pertussis, hepatitis b (hepb), and haemophilus influenzae type b (hib) (dtwp-hepb-hib) quinvaxem® vaccine in children. a total of 131 infants received the quinvaxem® vaccine at 2, 3 and 4 months. antibody levels were measured at baseline, at one ... | 2015 | 26867396 |
| is alveolar macrophage phagocytic dysfunction in children with protracted bacterial bronchitis a forerunner to bronchiectasis? | children with recurrent protracted bacterial bronchitis (pbb) and bronchiectasis share common features, and pbb is likely a forerunner to bronchiectasis. both diseases are associated with neutrophilic inflammation and frequent isolation of potentially pathogenic microorganisms, including nontypeable haemophilus influenzae (nthi), from the lower airway. defective alveolar macrophage phagocytosis of apoptotic bronchial epithelial cells (efferocytosis), as found in other chronic lung diseases, may ... | 2016 | 26867834 |
| environmental enteropathy, oral vaccine failure and growth faltering in infants in bangladesh. | environmental enteropathy (ee) is a subclinical enteric condition found in low-income countries that is characterized by intestinal inflammation, reduced intestinal absorption, and gut barrier dysfunction. we aimed to assess if ee impairs the success of oral polio and rotavirus vaccines in infants in bangladesh. | 2015 | 26870801 |
| bacteremia in children hospitalized with respiratory syncytial virus infection. | the risk of bacteremia is considered low in children with acute bronchiolitis. however the rate of occult bacteremia in infants with rsv infection is not well established. the aim was to determine the actual rate and predictive factors of bacteremia in children admitted to hospital due to confirmed rsv acute respiratory illness (ari), using both conventional culture and molecular techniques. | 2016 | 26872131 |
| lobar distribution in non-cystic fibrosis bronchiectasis predicts bacteriologic pathogen treatment. | non-cystic fibrosis bronchiectasis (ncfbr) is a major cause of morbidity due to frequent infectious exacerbations. we analyzed the influence of patient age and bronchiectasis location on the bacterial profile of patients with ncfbr. this retrospective cohort study included 339 subjects diagnosed with an infectious exacerbation of ncfbr during the 9-year period between january 2006 and december 2014. bronchoalveolar lavage (bal) cultures and high-resolution computed tomography scans (hrct) were u ... | 2016 | 26873379 |
| [multicenter prospective epidemiological studies on haemophilus influenzae infection among hospitalized children with lower respiratory tract infections]. | to understand epidemiological characteristics of haemophilus influenzae (hi) infection in hospitalized children with lower respiratory tract infection (lrti) in west sichuan china. | 2016 | 26875462 |
| conjugate haemophilus influenzae type b vaccines for sickle cell disease. | people affected with sickle cell disease are at high risk of infection from haemophilus influenzae type b. before the implementation of haemophilus influenzae type b conjugate vaccination in high-income countries, this was responsible for a high mortality rate in children under five years of age. in african countries, where coverage of this vaccination is still extremely low, haemophilus influenzae type b remains one of the most common cause of bacteraemias in children with sickle cell disease. ... | 2016 | 26881484 |
| oral polio vaccination and hospital admissions with non-polio infections in denmark: nationwide retrospective cohort study. | background. live vaccines may have nonspecific beneficial effects on morbidity and mortality. this study examines whether children who had the live-attenuated oral polio vaccine (opv) as the most recent vaccine had a different rate of admissions for infectious diseases than children with inactivated diphtheria-tetanus-pertussis-polio-haemophilus influenzae type b vaccine (dtap-ipv-hib) or live measles-mumps-rubella vaccine (mmr) as their most recent vaccine. methods. a nationwide, register-bas ... | 2016 | 26885538 |
| immunization coverage among splenectomized patients: results of an ad hoc survey in puglia region (south of italy). | patients with anatomic or functional asplenia have a 10-50 times higher risk than general population to develop overwhelming post-splenectomy infection. evidences are unanimous in recommending splenectomised patients to receive meningococcal, antipneumococcal and haemophilus influenzae type b vaccinations according to a specific timing. in italy there are no current data on the immunisation coverage in these patients. this study aims to investigate immunisation coverage in patients undergoing el ... | 2016 | 26890256 |
| developing a vaccine to prevent otitis media caused by nontypeable haemophilus influenzae. | nontypeable haemophilus influenzae (nthi) is a predominant organism of the upper respiratory nasopharyngeal microbiota. its disease spectrum includes otitis media, sinusitis, non-bacteremic pneumonia and invasive infections. protein-based vaccines to prevent nthi infections are needed to alleviate these infections in children and vulnerable populations such as the elderly and those with chronic obstructive pulmonary disease (copd). one nthi protein is included in a pneumococcal conjugate vaccine ... | 2016 | 26894630 |
| hib vaccines: past, present, and future perspectives. | haemophilus influenzae type b (hib) causes many severe diseases, including epiglottitis, pneumonia, sepsis, and meningitis. in developed countries, the annual incidence of meningitis caused by bacteria is approximately 5-10 cases per population of 100,000. the hib conjugate vaccine is considered protective and safe. adjuvants, molecules that can enhance and/or regulate the fundamental immunogenicity of an antigen, comprise a wide range of diverse compounds. while earlier developments of adjuvant ... | 2016 | 26904695 |
| maternal helminth infection is associated with higher infant immunoglobulin a titers to antigen in orally administered vaccines. | many studies have documented lower vaccine efficacy among children in low-income countries, compared with their counterparts in high-income countries. this disparity is especially apparent with respect to oral vaccines such as rotavirus and oral polio vaccines. one potential contributing factor is the presence of maternal antenatal helminth infections, which can modulate the infant's developing immune system. | 2016 | 26908751 |
| susceptibility to lower respiratory infections in childhood is associated with perturbation of the cytokine response to pathogenic airway bacteria. | neonatal colonization of the airways with respiratory pathogens is associated with increased risk of lower respiratory infections (lri) in early childhood. therefore, we hypothesized that children developing lri have an aberrant immune response to pathogenic bacteria in infancy. the objective was to characterize in vitro the early life systemic immune response to pathogenic bacteria and study the possible association with incidence of lri during the first 3 years of life. | 2016 | 26910587 |
| a glycoconjugate of haemophilus influenzae type b capsular polysaccharide with tetanus toxoid protein: hydrodynamic properties mainly influenced by the carbohydrate. | three important physical properties which may affect the performance of glycoconjugate vaccines against serious disease are molar mass (molecular weight), heterogeneity (polydispersity), and conformational flexibility in solution. the dilute solution behaviour of native and activated capsular polyribosylribitol (prp) polysaccharides extracted from haemophilus influenzae type b (hib), and the corresponding glycoconjugate made by conjugating this with the tetanus toxoid (tt) protein have been char ... | 2016 | 26915577 |
| prevalence, codetection and seasonal distribution of upper airway viruses and bacteria in children with acute respiratory illnesses with cough as a symptom. | most studies exploring the role of upper airway viruses and bacteria in paediatric acute respiratory infections (ari) focus on specific clinical diagnoses and/or do not account for virus-bacteria interactions. we aimed to describe the frequency and predictors of virus and bacteria codetection in children with ari and cough, irrespective of clinical diagnosis. bilateral nasal swabs, demographic, clinical and risk factor data were collected at enrollment in children aged <15 years presenting to an ... | 2016 | 26916343 |
| sex-dependent immune responses to infant vaccination: an individual participant data meta-analysis of antibody and memory b cells. | biological sex can be an important source of variation in infection and immunity and sex-dependent differences in immune response to vaccination have been reported in some studies. | 2016 | 26920472 |
| [vaccination for international travelers]. | traveler's vaccination is one of the key strategies for the prevention of infectious diseases during international travel. the risk of acquiring an infectious disease is determined in each case by the characteristics of the traveler and the travel, so the pre-departure medical advice of the traveler must be individualized. the world health organization classifies travelerś vaccines into three groups. - vaccines for routine use in national immunization programs: haemophilus influenzae type b, hep ... | 2016 | 26920587 |
| comparison of the immunogenicity and safety of pentavalent vaccine quinvaxem in a compact prefilled auto-disabled (cpad) injection system versus single-dose vials in healthy infants: a phase 3, open-label, randomized, parallel-group, non-inferiority study. | to evaluate non-inferiority of three doses of quinvaxem in a compact prefilled auto-disabled (cpad) injection system versus quinvaxem in a single-dose vial administered with conventional syringe in terms of seroconversion/seroprotection rates for all antibodies (anti-hepatitis b (hb), anti-haemophilus influenzae type b polyribosylribitol phosphate (hib prp), anti-diphtheria, anti-tetanus, anti-bordetella pertussis) at 1 month after primary vaccination. | 2016 | 26923083 |
| bacterial colonization and associated factors in patients with bronchiectasis. | to evaluate the bacterial colonization and associated risk factors in patients with bronchiectasis. | 2017 | 26933458 |
| immunization coverage surveys and linked biomarker serosurveys in three regions in ethiopia. | demographic and health surveys, immunization coverage surveys and administrative data often divergently estimate vaccination coverage, which hinders pinpointing districts where immunization services require strengthening. we assayed vaccination coverage in three regions in ethiopia by coverage surveys and linked serosurveys. | 2016 | 26934372 |
| the muc1 mucin specifically inhibits activation of the nlrp3 inflammasome. | muc1 is a cell membrane-associated mucin, expressed ubiquitously on the mucosal epithelia as well as by immune cells, that limits the inflammatory response to multiple pathogens. we have recently shown that muc1 controls inflammation resulting from helicobacter pylori infection by suppressing interleukin-1β (il-1β) produced via the nlrp3 inflammasome. here, we demonstrate that muc1 also regulates il-1β secretion induced by the nlrp3-activating bacteria haemophilus influenzae but not bacteria tha ... | 2016 | 26938663 |
| community-acquired haemophilus influenzae pneumonia--new insights from the capnetz study. | we aimed to identify clinical characteristics and to assess effectiveness of different initial antibiotic regimens in adult patients with community-acquired pneumonia (cap) caused by haemophilus influenzae. | 2016 | 26940505 |
| haemophilus influenzae biofilm formation in chronic otitis media with effusion. | otitis media with effusion (ome) is a highly prevalent disease in children, but the exact pathogenesis and role of bacteria are still not well understood. this study aimed to investigate the presence of otopathogenic bacteria in the middle ear effusion (mee) and adenoid of children with chronic ome (come), and to investigate in vivo whether these bacteria, especially haemophilus influenzae, are organized as a biofilm in the middle ear fluid. mee and adenoid samples were collected from 21 patient ... | 2016 | 26946303 |
| clarithromycin resistance mechanisms of epidemic β-lactamase-nonproducing ampicillin-resistant haemophilus influenzae strains in japan. | the aim of this study was to clarify the clarithromycin resistance mechanisms of β-lactamase-nonproducing ampicillin-resistant haemophilus influenzae strains. in all clarithromycin-resistant strains, the transcript level of acrb was significantly elevated, and these strains had a frameshift mutation in acrr introduction of the acrr mutation into h. influenzae rd generated a clarithromycin-resistant transformant with the same mic as the donor strain. our results indicate that the acrr mutation co ... | 2016 | 26953210 |
| crystal structure of a complex of surfactant protein d (sp-d) and haemophilus influenzae lipopolysaccharide reveals shielding of core structures in sp-d-resistant strains. | the carbohydrate recognition domains (crds) of lung collectin surfactant protein d (sp-d) recognize sugar patterns on the surface of lung pathogens and promote phagocytosis. using haemophilus influenzae eagan strains expressing well-characterized lipopolysaccharide (lps) surface structures of various levels of complexity, we show that bacterial recognition and binding by sp-d is inversely related to lps chain extent and complexity. the crystal structure of a biologically active recombinant trime ... | 2016 | 26953329 |
| predominant bacteria detected from the middle ear fluid of children experiencing otitis media: a systematic review. | otitis media (om) is amongst the most common childhood diseases and is associated with multiple microbial pathogens within the middle ear. global and temporal monitoring of predominant bacterial pathogens is important to inform new treatment strategies, vaccine development and to monitor the impact of vaccine implementation to improve progress toward global om prevention. | 2016 | 26953891 |
| effect of pneumococcal conjugate vaccine on the natural antibodies and antibody responses against protein antigens from streptococcus pneumoniae, haemophilus influenzae and moraxella catarrhalis in children with community-acquired pneumonia. | streptococcus pneumoniae, haemophilus influenzae and moraxella catarrhalis are common causative agents of respiratory infections. pneumococcal conjugate vaccines have been introduced recently, but their effect on the natural immunity against protein antigens from these pathogens has not been elucidated. | 2016 | 26954601 |
| safety profile of the 10-valent pneumococcal non-typeable haemophilus influenzae protein d conjugate vaccine (phid-cv). | safety and reactogenicity data were reviewed following 10 years of experience with the 10-valent pneumococcal non-typeable haemophilus influenzae protein d conjugate vaccine (phid-cv) in clinical development and from post-licensure settings. analyses of pooled clinical trial data and post-marketing reports provided an overview of its safety profile and allowed assessment of rare adverse events that might not have been identified previously. the safety of phid-cv was also evaluated in children at ... | 2017 | 26954689 |
| [toward a new immunization schedule in spain, 2016 (part 1)]. | the immunization schedule is a dynamic public health tool that has incorporated different changes over the years influenced by the epidemiologic situation and the scientific evidence. the immunization advisory committee [ponencia de programa y registro de vacunaciones], as the interterritorial council scientific and technical advisory body, carries out assessments of different programmes and vaccines and proposes changes that after approval will be introduced in the regions schedule. this articl ... | 2016 | 26960253 |
| [toward a new immunization schedule in spain, 2016 (part 2)]. | immunization schedules are intrinsically dynamic in order to embed the immunologic and epidemiologic changes in any specific geographic region. according to this, the current study addresses a proposal to modify the childhood immunization schedule in spain. in order to move from a three plus one schema to a two plus one, we undertake a review of the available literature to explore the immunological and clinical rationale behind this change, including an overview of the potential impact on this s ... | 2016 | 26960345 |
| intra-tracheal administration of haemophilus influenzae in mouse models to study airway inflammation. | here, we describe a detailed procedure to efficiently and directly deliver haemophilus influenzae into the lower respiratory tracts of mice. we demonstrate the procedure for preparing h. influenzae inoculum, intra-tracheal instillation of h. influenzae into the lung, collection of broncho-alveolar lavage fluid (balf), analysis of immune cells in the balf, and rna isolation for differential gene expression analysis. this procedure can be used to study the lung inflammatory response to any bacteri ... | 2016 | 26967704 |
| serum cytokine biomarkers accurately predict presence of acute otitis media infection and recovery caused by haemophilus influenzae. | we sought to develop an optimal model using a combination of serum biomarker pro-inflammatory and dampening inflammatory cytokine proteins to predict the presence of acute otitis media (aom) infection and recovery caused by nontypeable haemophilus influenzae (nthi). | 2016 | 26968077 |
| [bacterial etiology of acute otitis media in spain in the post-pneumococcal conjugate vaccine era]. | acute otitis media (aom) is common in children aged <3 years. a pneumococcal conjugate vaccine (pcv) (pcv7; prevenar, pfizer/wyeth, usa) has been available in spain since 2001, which has a coverage rate of 50-60% in children aged <5 years. | 2016 | 26969247 |
| detection of respiratory pathogens in pediatric acute otitis media by pcr and comparison of findings in the middle ear and nasopharynx. | we conducted a series of polymerase chain reactions (pcrs) in order to detect bacteria (7 species) and viruses (17 species) in middle ear fluid (mef) and nasopharynx (nph) of children with acute otitis media (aom; n=179). bacterial and viral nucleic acids were detected in mef of 78.8% and 14.5% patients, respectively. the prevalence was as follows: streptococcus pneumoniae, 70.4%; haemophilus influenzae, 17.9%; staphylococcus aureus, 16.8%; streptococcus pyogenes, 12.3%; moraxella catarrhalis, 9 ... | 2016 | 26971180 |
| identification and characterization of msf, a novel virulence factor in haemophilus influenzae. | haemophilus influenzae is an opportunistic pathogen. the emergence of virulent, non-typeable strains (nthi) emphasizes the importance of developing new interventional targets. we screened the nthi supragenome for genes encoding surface-exposed proteins suggestive of immune evasion, identifying a large family containing sel1-like repeats (slrs). clustering identified ten slr-containing gene subfamilies, each with various numbers of slrs per gene. individual strains also had varying numbers of slr ... | 2016 | 26977929 |
| assessing vaccine data recording in brazil. | vaccines represent an important advancement for improving the general health of a population. the effective recording of vaccine data is a factor for the definition of its supply chain. this study investigated vaccine data recording relatively to data collected from vaccination rooms and data obtained from a government-developed internet platform. | 2016 | 26982292 |
| immunogenicity and safety of two doses of catch-up immunization with haemophilus influenzae type b conjugate vaccine in indian children living with hiv. | children living with hiv are at increased risk of disease from haemophilus influenzae type b (hib). data are limited on the immunogenicity of a two-dose, catch-up schedule for hib conjugate vaccine (hibcv) among hiv-infected children accessing antiretroviral therapy (art) late. | 2016 | 26988256 |
| surveillance of antimicrobial resistance in lebanese hospitals: retrospective nationwide compiled data. | antimicrobial resistance is closely linked to antimicrobial use and is a growing concern worldwide. antimicrobial resistance increases healthcare costs substantially in many countries, including lebanon. national data from lebanon have, in the most part, been limited to a few academic hospitals. the lebanese society of infectious diseases conducted a retrospective study to better describe the antimicrobial susceptibility patterns of bacterial isolates in lebanon. data were based on records retri ... | 2016 | 26996458 |
| novel strategy to protect against influenza virus-induced pneumococcal disease without interfering with commensal colonization. | streptococcus pneumoniae commonly inhabits the nasopharynx as a member of the commensal biofilm. infection with respiratory viruses, such as influenza a virus, induces commensal s. pneumoniae to disseminate beyond the nasopharynx and to elicit severe infections of the middle ears, lungs, and blood that are associated with high rates of morbidity and mortality. current preventive strategies, including the polysaccharide conjugate vaccines, aim to eliminate asymptomatic carriage with vaccine-type ... | 2016 | 27001538 |
| self-reported prenatal influenza vaccination and early childhood vaccine series completion. | no studies have examined associations between prenatal vaccination and childhood vaccination. mothers who refuse influenza vaccinations during pregnancy report similar attitudes and beliefs to those who refuse vaccinations for their children. the objective of this study was to examine the association between self-reported prenatal influenza vaccination and early childhood vaccination. | 2016 | 27002252 |
| clinical and microbiologic characteristics of vulvovaginitis in korean prepubertal girls, 2009-2014: a single center experience. | to update information on the clinical and microbiologic characteristics of pediatric vulvovaginitis in korean prepubertal girls. | 2016 | 27004204 |
| multi-site placebo-controlled randomised clinical trial to assess protection following oral immunisation with inactivated non-typeable haemophilus influenzae in chronic obstructive pulmonary disease. | previous studies identified factors that modify response to an oral non-typeable haemophilus influenzae (nthi) vaccine in chronic obstructive pulmonary disease (copd): severe copd, moderate-severe exacerbations as end-point and a threshold prevalence of nthi in the study population. more data are needed to confirm parameters that influence clinical outcomes. | 2016 | 27009822 |
| the first report: an analysis of bacterial flora of the first voided urine specimens of patients with male urethritis using the 16s ribosomal rna gene-based clone library method. | to analyse the bacterial flora of urine from patients with male urethritis using the clone library method. | 2016 | 27013259 |
| prevention of early episodes of otitis media by pneumococcal vaccines might reduce progression to complex disease. | otitis media is a common childhood infection of the middle ear and a major cause of morbidity. this multifactorial disease manifests as a spectrum of clinical syndromes from uncomplicated acute otitis media to more complex recurrent and chronic cases (frequently polymicrobial), with the major pathogens involved being streptococcus pneumoniae and non-typeable haemophilus influenzae. pneumococcal conjugate vaccines (pcvs) target only a few serotypes that cause otitis media; however, results from s ... | 2016 | 27036355 |
| results from the solithromycin international surveillance program (2014). | solithromycin, a fourth-generation macrolide (a fluoroketolide with enhanced activity against macrolide-resistant bacteria due to interaction with three ribosomal sites) and the first fluoroketolide, was tested against a 2014 collection of 6,115 isolates, including streptococcus pneumoniae (1,713 isolates), haemophilus influenzae (1,308), moraxella catarrhalis (577), staphylococcus aureus (1,024), and beta-hemolytic streptococci (1,493), by reference broth microdilution methods. the geographic s ... | 2016 | 27044551 |
| type iv pilus expression is upregulated in nontypeable haemophilus influenzae biofilms formed at the temperature of the human nasopharynx. | nontypeable haemophilus influenzae (nthi), a commensal of the human nasopharynx (hnp), is a common cause of biofilm-associated diseases of the respiratory tract. however, nthi biofilm biology at the average hnp temperature, i.e., 34°c, has not been well studied. here we grew nthi biofilms at 34°c and 37°c, to evaluate relative biofilm growth, expression, and function of the type iv pilus (tfp), a critical adhesin important for nthi biofilm formation. the kinetics and regulation of tfp expression ... | 2016 | 27044626 |
| bronchiectasis: a bacteriological profile. | the occurrence of bronchiectasis can involve a combination of many environmental factors, including infection. the aim of our work is to determine the bacteriological profile of bronchiectasis. this is a retrospective study of 100 patients hospitalized in between january 2010 and july 2013. the average age was 48 years with a 58% female predominance. symptomatology was by a bronchial syndrome in 90% of cases. bacteriological examination was able to isolate the microbe in 35% of cases. in our stu ... | 2015 | 27047618 |
| antibiotic susceptibility in streptococcus pneumoniae, haemophilus influenzae and streptococcus pyogenes in pakistan: a review of results from the survey of antibiotic resistance (soar) 2002-15. | to investigate changes in the antibiotic susceptibility of streptococcus pneumoniae, haemophilus influenzae and streptococcus pyogenes from the survey of antibiotic resistance (soar) in community-acquired respiratory tract infections (ca-rtis) between 2002 and 2015 in pakistan. | 2016 | 27048578 |
| results from the survey of antibiotic resistance (soar) 2011-14 in the democratic republic of congo, ivory coast, republic of senegal and kenya. | to assess antibiotic susceptibility of community-acquired respiratory tract isolates from ivory coast, kenya, democratic republic of congo (drc) and senegal in 2011-14. | 2016 | 27048579 |
| results from the survey of antibiotic resistance (soar) 2012-14 in thailand, india, south korea and singapore. | to provide susceptibility data for community-acquired respiratory tract isolates of streptococcus pneumoniae, streptococcus pyogenes, haemophilus influenzae and moraxella catarrhalis collected in 2012-14 from four asian countries. | 2016 | 27048580 |
| results from the survey of antibiotic resistance (soar) 2009-11 and 2013-14 in china. | to compare antibiotic susceptibility of community-acquired respiratory bacteria in china during 2009-11 and 2013-14. | 2016 | 27048581 |
| results from the survey of antibiotic resistance (soar) 2011-13 in the gulf states. | to provide surveillance data on the susceptibility of community-acquired respiratory tract isolates from four gulf and near east countries from 2011 to 2013. | 2016 | 27048582 |
| results from the survey of antibiotic resistance (soar) 2011-13 in ukraine. | to determine the antibiotic susceptibility of respiratory isolates of streptococcus pneumoniae and haemophilus influenzae collected in 2011-13 from ukraine. | 2016 | 27048583 |
| results from the survey of antibiotic resistance (soar) 2011-13 in turkey. | data are presented from the survey of antibiotic resistance (soar) for respiratory tract infection pathogens collected in 2011-13 from turkey. | 2016 | 27048584 |
| results from the survey of antibiotic resistance (soar) 2002-09 in turkey. | to investigate changes in antibiotic susceptibility of streptococcus pneumoniae and haemophilus influenzae from the survey of antibiotic resistance (soar) in community-acquired respiratory tract infections (ca-rtis) between 2002 and 2009 in turkey. | 2016 | 27048585 |
| results from the survey of antibiotic resistance (soar) 2009-11 in vietnam. | to investigate the susceptibility of respiratory tract infection pathogens collected between 2009 and 2011 from the soar study in vietnam. | 2016 | 27048586 |
| ct-abnormalities, bacteriology and symptoms of sinonasal disease in children with cystic fibrosis. | sinonasal pathology in adults with cystic fibrosis (cf) is common but the extent of ct-abnormalities and symptoms of sinonasal disease in children with cf and the age of onset are less frequently studied. | 2016 | 27049043 |
| delayed bcg immunization does not alter antibody responses to epi vaccines in hiv-exposed and -unexposed south african infants. | bacille calmette-guérin (bcg) is routinely given at birth in tuberculosis-endemic settings due to its protective effect against disseminated tuberculosis in infants. bcg is however contraindicated in hiv-infected infants. we investigated whether delaying bcg vaccination to 14 weeks of age affected vaccine-induced antibody responses to haemophilus influenzae type b (hib)-conjugate, pertussis, tetanus and hepatitis b (hbv) vaccines, in hiv-exposed uninfected (heu) and -unexposed uninfected (huu) i ... | 2016 | 27055019 |
| estimating the public health importance of the cyd-tetravalent dengue vaccine: vaccine preventable disease incidence and numbers needed to vaccinate. | to evaluate the potential public health impact of the live attenuated tetravalent sanofi pasteur dengue vaccine (cyd-tdv) we analyzed data from the reported clinical trials to calculate vaccine preventable disease incidence (vpdi) and number needed to vaccinate (nnv) based on the licensure indication for persons age 9 years and above. | 2016 | 27055020 |
| listeria monocytogenes merr-like regulator nmlrlm: its transcriptome and role in stress response. | nmlr, a negative transcription regulator in the merr family, is involved in oxidative and nitrosative stress response in neisseria gonorrhoeae and haemophilus influenzae. in this study, the objective was to characterize the role and the regulon of nmlr in the foodborne listeria monocytogenes. an l. monocytogenes nmlr null mutant strain was constructed. transcriptomes of strain 10403s wild type (wt) and δnmlrlm strains grown to the stationary phase were determined by mrna sequencing. differential ... | 2016 | 27058117 |
| erratum for baddal et al., dual rna-seq of nontypeable haemophilus influenzae and host cell transcriptomes reveals novel insights into host-pathogen cross talk. | 2016 | 27073092 | |
| streptococcus pneumoniae and haemophilus influenzae type b carriage in chinese children aged 12-18 months in shanghai, china: a cross-sectional study. | the bacteria streptococcus pneumoniae (pneumococcus) and haemophilus influenzae type b (hib) are leading causes of childhood pneumonia and meningitis and are major contributors to worldwide mortality in children younger than 5 years of age. asymptomatic nasopharyngeal carriage of pneumococcus and hib was determined for healthy children in shanghai in 2009. | 2016 | 27080523 |
| altered expression of the ccn genes in the lungs of mice in response to cigarette smoke exposure and viral and bacterial infections. | the ccn proteins are key signaling and regulatory molecules involved in many biological functions and contribute to malignant and non-malignant lung diseases. despite the high morbidity and mortality of the lung respiratory infectious diseases, there is very little data related to the expression of the ccns during infection. we investigated in mice the pulmonary mrna expression levels of five ccns (1 to 5) in response to influenza a virus (iav) and bacterial agents (nontypeable haemophilus influ ... | 2016 | 27080955 |
| microbiology of chronic rhinosinusitis. | most sinus infections are viral and only a small percentage develop bacterial infection. rhino-, influenza, and para-influenza viruses are the most frequent viral causes of sinusitis. the most common bacterial isolates from children and adult patients with community-acquired acute bacterial sinusitis are streptococcus pneumoniae, haemophilus influenzae, moraxella catarrhalis, and streptococcus pyogenes. staphylococcus aureus and anaerobic organisms (prevotella and porphyromonas, fusobacterium, a ... | 2016 | 27086363 |
| bacterial tracheitis caused by haemophilus influenzae after influenza. | 2016 | 27086830 | |
| retrospective analysis of the microbiological spectrum of pneumonia in turkish patients with lung cancer. | the spectrum of pulmonary infections in patients with lung cancer is wide, and tools for target-oriented infection control measures are necessary. in this retrospective study we report the microbiological spectrum of pneumonia (based on the results of sputum culture) in a case series of turkish patients with lung malignancies. | 2016 | 27095942 |
| disease burden of 32 infectious diseases in the netherlands, 2007-2011. | infectious disease burden estimates provided by a composite health measure give a balanced view of the true impact of a disease on a population, allowing the relative impact of diseases that differ in severity and mortality to be monitored over time. this article presents the first national disease burden estimates for a comprehensive set of 32 infectious diseases in the netherlands. | 2016 | 27097024 |
| immunogenicity, safety and reactogenicity of a booster dose of the 10-valent pneumococcal nontypeable h. influenzae protein d conjugate vaccine coadministered with dtpa-ipv-hib in dutch children: a randomized controlled trial. | immune responses and safety profiles may be affected when vaccines are coadministered. we evaluated the immunogenicity, safety and reactogenicity of a booster dose of the 10-valent pneumococcal nontypeable haemophilus influenzae protein d-conjugate (phid-cv; synflorix gsk vaccines) and dtpa-ipv-hib (pediacel sanofi pasteur msd) when coadministered. | 2016 | 27097348 |
| vaccinations in asplenic adults. | the recommended immunizations for adult asplenic patients are reviewed. | 2016 | 27099328 |
| 3d reconstruction of the human airway mucosa in vitro as an experimental model to study nthi infections. | we have established an in vitro 3d system which recapitulates the human tracheo-bronchial mucosa comprehensive of the pseudostratified epithelium and the underlying stromal tissue. in particular, we reported that the mature model, entirely constituted of primary cells of human origin, develops key markers proper of the native tissue such as the mucociliary differentiation of the epithelial sheet and the formation of the basement membrane. the infection of the pseudo-tissue with a strain of nonty ... | 2016 | 27101006 |