Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| photosensing fungi: phytochrome in the spotlight. | red light triggers asexual development and represses sexual development in the fungus aspergillus nidulans. this response has been shown to require a phytochrome red/far-red light photoreceptor, fpha, which is cytoplasmic and binds a tetrapyrrole chromophore. fpha exhibits similarities to both plant and bacterial phytochromes. | 2005 | 16243020 |
| the aspergillus nidulans phytochrome fpha represses sexual development in red light. | phytochrome photoreceptors sense red and far-red light through photointerconversion between two stable conformations, a process mediated by a linear tetrapyrrole chromophore. originally, phytochromes were thought to be confined to photosynthetic organisms including cyanobacteria, but they have been recently discovered in heterotrophic bacteria and fungi, where little is known about their functions. it was shown previously in the ascomycetous fungus aspergillus nidulans that asexual sporulation i ... | 2005 | 16243030 |
| protein o-mannosyltransferase a of aspergillus awamori is involved in o-mannosylation of glucoamylase i. | industrially important extracellular enzymes from filamentous fungi are often o-mannosylated. the structure and function of the pmta (aapmta) gene encoding the protein o-d-mannosyltransferase of aspergillus awamori were characterized. the aapmta disruptant, designated aapmta, was constructed by homologous recombination. the strain aapmta exhibited fragile cell morphology with respect to hyphal extension, as well as swollen hyphae formation and conidia formation in potato dextrose medium. moreove ... | 2005 | 16272387 |
| surface ultrastructure and elasticity in growing tips and mature regions of aspergillus hyphae describe wall maturation. | this study reports the first direct, high-resolution physical and structural evidence of wall changes during hyphal tip growth, visualized by atomic force microscopy (afm) in aspergillus nidulans. images from afm and cryo-scanning electron microscopy provided comparable information, but afm was also able to image and physically probe living cells. afm images showed changes in the surface ultrastructure of a. nidulans hyphae, from newly deposited walls at hyphal tips to fully mature walls, as wel ... | 2005 | 16272389 |
| efficient pcr-based gene targeting with a recyclable marker for aspergillus nidulans. | the rapid accumulation of genomic sequences from a large number of eukaryotes, including numerous filamentous fungi, has created a tremendous scientific potential, which can only be realized if precise site-directed genome modifications, like gene deletions, promoter replacements, in-frame gfp fusions and specific point mutations can be made rapidly and reliably. the development of gene-targeting techniques in filamentous fungi and other higher eukaryotes has been hampered because foreign dna is ... | 2006 | 16289954 |
| tracing the endocytic pathway of aspergillus nidulans with fm4-64. | simple procedures using fm4-64 to follow membrane internalization and transport to the vacuolar system and endomembranes in aspergillus nidulans are described. fm4-64 internalization is energy, temperature and f-actin dependent, strongly suggesting that it occurs by endocytosis. the dye sequentially labels: (i) cortical punctuate organelles whose motility resembles that of yeast actin patches; (ii) approximately 0.7 microm circular, hollow structures representing mature endosome/vacuole; and (ii ... | 2005 | 16291501 |
| g protein-coupled receptor gpr4 senses amino acids and activates the camp-pka pathway in cryptococcus neoformans. | the galpha protein gpa1 governs the camp-pka signaling pathway and plays a central role in virulence and differentiation in the human fungal pathogen cryptococcus neoformans, but the signals and receptors that trigger this pathway were unknown. we identified seven putative proteins that share identity with known g protein-coupled receptors (gpcrs). one protein, gpr4, shares limited sequence identity with the dictyostelium discoideum camp receptor car1 and the aspergillus nidulans gpcr protein gp ... | 2006 | 16291861 |
| deletions of endocytic components vps28 and vps32 affect growth at alkaline ph and virulence through both rim101-dependent and rim101-independent pathways in candida albicans. | ambient ph signaling involves a cascade of conserved rim or pal products in ascomycetous yeasts or filamentous fungi, respectively. recent evidences in the fungi aspergillus nidulans, saccharomyces cerevisiae, yarrowia lipolytica, and candida albicans suggested that components of endosomal sorting complexes required for transport (escrt) involved in endocytic trafficking were needed for signal transduction along the rim pathway. in this study, we confirm these findings with c. albicans and show ... | 2005 | 16299290 |
| c21orf5, a new member of dopey family involved in morphogenesis, could participate in neurological alterations and mental retardation in down syndrome. | availability of the human genome sequence promises important progress in the understanding of human pathologies, particularly for multifactorial diseases. among these, down syndrome (ds) is the most frequent genetic cause of mental retardation. a critical region of chromosome 21, the down syndrome chromosomal region-1 (dcr-1), is responsible for many features of the ds phenotype including mental retardation. we studied dcr-1 c21orf5 as a new candidate gene for ds considering its restricted expre ... | 2005 | 16303751 |
| genetic transformation of an argb mutant of aspergillus oryzae. | an argb mutant of aspergillus oryzae nrrl 492 has been genetically transformed with the aspergillus nidulans argb gene. protoplasts were generated with a combination of novozyme 234 and beta-glucuronidase and regenerated on sucrose-stabilized minimal medium without arginine as described for a. nidulans. a frequency of 5 to 10 transformants per mug of dna was obtained; however, most transformants appeared abortive. the a. nidulans argb gene and vector sequences appeared to be integrated into the ... | 1988 | 16347669 |
| isolation and characterization of sexual spore pigments from aspergillus nidulans. | the homothallic ascomycete aspergillus nidulans produces two types of pigmented spores: conidia and ascospores. the synthesis and localization of the spore pigments is developmentally regulated and occurs in specialized cell types. on the basis of spectroscopic evidence, we propose that the major ascospore pigment of a. nidulans (ascoquinone a) is a novel dimeric hydroxylated anthraquinone. the structure of ascoquinone a, as well as a comparison to model compounds, suggests that it is the produc ... | 1994 | 16349224 |
| a mapk gene from dead sea fungus confers stress tolerance to lithium salt and freezing-thawing: prospects for saline agriculture. | the dead sea is one of the most saline lakes on earth ( approximately 340 g/liter salinity) and is approximately 10 times saltier than the oceans. eurotium herbariorum, a common fungal species, was isolated from its water. ehhog gene, encoding a mitogen-activated protein kinase (mapk) that plays an essential role in the osmoregulatory pathway in yeast and many other eukaryotes, was isolated from e. herbariorum. the deduced amino acid sequences of ehhog indicated high similarity with homologous g ... | 2005 | 16365289 |
| comparison of gene expression signatures of diamide, h2o2 and menadione exposed aspergillus nidulans cultures--linking genome-wide transcriptional changes to cellular physiology. | in addition to their cytotoxic nature, reactive oxygen species (ros) are also signal molecules in diverse cellular processes in eukaryotic organisms. linking genome-wide transcriptional changes to cellular physiology in oxidative stress-exposed aspergillus nidulans cultures provides the opportunity to estimate the sizes of peroxide (o2(2-)), superoxide (o2*-) and glutathione/glutathione disulphide (gsh/gssg) redox imbalance responses. | 2005 | 16368011 |
| genetic transformation of ascochyta rabiei using agrobacterium-mediated transformation. | in order to study pathogenic mechanisms of the plant pathogen ascochyta rabiei, conditions for efficient transformation using agrobacterium-mediated transformation were investigated. hygromycin b resistance (hph) was superior to geneticin resistance (nptii) for selecting transformants, and the hph gene was more efficiently expressed by the aspergillus nidulans trpc promoter than by the cauliflower mosaic virus 35s promoter camv35s. co-cultivation on solid media for 72 h was optimal for generatin ... | 2006 | 16369840 |
| sequencing of aspergillus nidulans and comparative analysis with a. fumigatus and a. oryzae. | the aspergilli comprise a diverse group of filamentous fungi spanning over 200 million years of evolution. here we report the genome sequence of the model organism aspergillus nidulans, and a comparative study with aspergillus fumigatus, a serious human pathogen, and aspergillus oryzae, used in the production of sake, miso and soy sauce. our analysis of genome structure provided a quantitative evaluation of forces driving long-term eukaryotic genome evolution. it also led to an experimentally va ... | 2005 | 16372000 |
| genome sequencing and analysis of aspergillus oryzae. | the genome of aspergillus oryzae, a fungus important for the production of traditional fermented foods and beverages in japan, has been sequenced. the ability to secrete large amounts of proteins and the development of a transformation system have facilitated the use of a. oryzae in modern biotechnology. although both a. oryzae and aspergillus flavus belong to the section flavi of the subgenus circumdati of aspergillus, a. oryzae, unlike a. flavus, does not produce aflatoxin, and its long histor ... | 2005 | 16372010 |
| analysis of the aspergillus nidulans thaumatin-like ceta gene and evidence for transcriptional repression of pyr4 expression in the ceta-disrupted strain. | the asexual spore or conidium plays a critical role in the life cycle of many filamentous fungi. however, the process of conidial germination remains surprisingly obscure. to better understand this process at the molecular level we characterized the aspergillus nidulans ceta gene which is uniquely transcribed in conidiating cultures and whose transcript is significantly enriched in mature conidia. ceta is a member of a novel family of fungal genes of unknown function with homology to plant thaum ... | 2006 | 16376592 |
| development of a modified positive selection medium that allows to isolate aspergillus oryzae strains cured of the integrated niad-based plasmid. | the nitrate reductase gene (niad) is the most frequently utilized as a selectable marker for homologous integration at the niad locus of aspergillus oryzae. in this study we developed a method for curing of the niad-based plasmid integrated on the a. oryzae genome. positive selection using a modified chlorate medium containing leucine as a nitrogen source enabled efficient isolation of the strains deficient in nitrate assimilation from the niad(+) transformant. pcr analysis of the strains confir ... | 2005 | 16377911 |
| visualization of the endocytic pathway in the filamentous fungus aspergillus oryzae using an egfp-fused plasma membrane protein. | endocytosis is an important process for cellular activities. however, in filamentous fungi, the existence of endocytosis has been so far elusive. in this study, we used aouapc-egfp, the fusion protein of a putative uric acid-xanthine permease with enhanced green fluorescent protein (egfp) in aspergillus oryzae, to examine whether the endocytic process occurs or not. upon the addition of ammonium into the medium the fusion protein was internalized from the plasma membrane. the internalization of ... | 2006 | 16380079 |
| flug-dependent asexual development in aspergillus nidulans occurs via derepression. | the asexual spore is one of the most crucial factors contributing to the fecundity and fitness of filamentous fungi. although the developmental activator flug was shown to be necessary for activation of asexual sporulation (conidiation) and production of the carcinogenic mycotoxin sterigmatocystin (st) in the model filamentous fungus aspergillus nidulans, the molecular mechanisms underlying the developmental switch have remained elusive. in this study, we report that the flug-mediated conidiatio ... | 2006 | 16387865 |
| a versatile and efficient gene-targeting system for aspergillus nidulans. | aspergillus nidulans is an important experimental organism, and it is a model organism for the genus aspergillus that includes serious pathogens as well as commercially important organisms. gene targeting by homologous recombination during transformation is possible in a. nidulans, but the frequency of correct gene targeting is variable and often low. we have identified the a. nidulans homolog (nkua) of the human ku70 gene that is essential for nonhomologous end joining of dna in double-strand b ... | 2006 | 16387870 |
| the gata factor area regulates localization and in vivo binding site occupancy of the nitrate activator nira. | the gata factor area is a wide-domain regulator in aspergillus nidulans with transcriptional activation and chromatin remodelling functions. area interacts with the nitrate-specific zn(2)-c(6) cluster protein nira and both proteins cooperate to synergistically activate nitrate-responsive genes. we have previously established that nira in vivo dna binding site occupancy is area dependent and in this report we provide a mechanistic explanation for our previous findings. we now show that area regul ... | 2006 | 16390440 |
| distinct ceramide synthases regulate polarized growth in the filamentous fungus aspergillus nidulans. | in filamentous fungi, the stabilization of a polarity axis is likely to be a pivotal event underlying the emergence of a germ tube from a germinating spore. recent results implicate the polarisome in this process and also suggest that it requires localized membrane organization. here, we employ a chemical genetic approach to demonstrate that ceramide synthesis is necessary for the formation of a stable polarity axis in the model fungus aspergillus nidulans. we demonstrate that a novel compound ( ... | 2006 | 16394102 |
| the presence of gc-ag introns in neurospora crassa and other euascomycetes determined from analyses of complete genomes: implications for automated gene prediction. | a combination of experimental and computational approaches was employed to identify introns with noncanonical gc-ag splice sites (gc-ag introns) within euascomycete genomes. evaluation of 2335 cdna-confirmed introns from neurospora crassa revealed 27 such introns (1.2%). a similar frequency (1.0%) of gc-ag introns was identified in fusarium graminearum, in which 3 of 292 cdna-confirmed introns contained gc-ag splice sites. computational analyses of the n. crassa genome using a gc-ag intron conse ... | 2006 | 16406724 |
| genomics reveals traces of fungal phenylpropanoid-flavonoid metabolic pathway in the f ilamentous fungus aspergillus oryzae. | fungal secondary metabolites constitute a wide variety of compounds which either play a vital role in agricultural, pharmaceutical and industrial contexts, or have devastating effects on agriculture, animal and human affairs by virtue of their toxigenicity. owing to their beneficial and deleterious characteristics, these complex compounds and the genes responsible for their synthesis have been the subjects of extensive investigation by microbiologists and pharmacologists. a majority of the funga ... | 2005 | 16410762 |
| screening of growth- or development-related genes by using genomic library with inducible promoter in aspergillus nidulans. | using the genomic library constructed at the downstream of the niia promoter, which induces the over-expression of an inserted dna fragment, we have attempted to screen the genes affecting growth or development by over-expression. the wild-type strain was transformed using the ama-niia(p) library and cultured on 1.2 m sorbitol media, in which asexual sporulation is induced, but sexual development is repressed. over 100,000 strains transformed to pyrg(+) were analyzed with regard to any changes i ... | 2005 | 16410769 |
| regulation of hyphal morphogenesis and the dna damage response by the aspergillus nidulans atm homolog atma. | ataxia telangiectasia (a-t) is an inherited disorder characterized by progressive loss of motor function and susceptibility to cancer. the most prominent clinical feature observed in a-t patients is the degeneration of purkinje motor neurons. numerous studies have emphasized the role of the affected gene product, atm, in the regulation of the dna damage response. however, in purkinje cells, the bulk of atm localizes to the cytoplasm and may play a role in vesicle trafficking. the nature of this ... | 2006 | 16415361 |
| identification of the mste gene encoding a glucose-inducible, low affinity glucose transporter in aspergillus nidulans. | the mste gene encoding a low affinity glucose transporter active during the germination of aspergillus nidulans conidia on glucose medium has been identified. mste expression also occurs in hyphae, is induced in the presence of other repressing carbon sources besides glucose, and is dependent on the function of the transcriptional repressor crea. the expression of mste and its subcellular distribution have been studied using a mste-sgfp fusion protein. concordant with data on mste expression, ms ... | 2006 | 16418173 |
| farnesol-induced apoptosis in aspergillus nidulans reveals a possible mechanism for antagonistic interactions between fungi. | the dimorphic fungus candida albicans secretes farnesol, which acts as a quorum-sensing molecule and prevents the yeast to mycelium conversion. in this study we examined the effect of farnesol in the filamentous fungus aspergillus nidulans. we show that externally added farnesol has no effect on hyphal morphogenesis; instead, it triggers morphological features characteristic of apoptosis. additional experiments suggest that mitochondria and reactive oxygen species (ros) participate in farnesol-i ... | 2006 | 16420349 |
| tmpa, a member of a novel family of putative membrane flavoproteins, regulates asexual development in aspergillus nidulans. | asexual reproduction (conidiation) in aspergillus nidulans is induced by environmental signals like exposure to air or nutrient starvation, and depends on brla gene activation. the study of 'fluffy' mutants showing delayed asexual development and reduced brla expression has defined the flug pathway, involved in regulation of this differentiation process. genetic characterization of a 'fluffy' mutant identified tmpa as a new gene involved in regulation of conidiation. tmpa defines a new family of ... | 2006 | 16420356 |
| oxylipins act as determinants of natural product biosynthesis and seed colonization in aspergillus nidulans. | secreted, hormone-like lipogenic molecules, called oxylipins, mediate the balance of asexual to sexual spore ratio in aspergillus nidulans. oxylipin production in this fungus is dependent on developmental regulation of three conserved fatty acid oxygenases, ppoa, ppob and ppoc. here, we show that in addition to altering spore ratios, loss of ppo genes affect natural product biosynthesis and seed colonization. deltappoa;deltappoc and deltappoa;deltappob;deltappoc mutants were unable to produce th ... | 2006 | 16420358 |
| a global view of metabolites. | fungi are rich sources of medically useful, as well as toxic, secondary metabolites. in this issue of chemistry & biology, bok et al. show that, in aspergillus nidulans, microarray analysis and manipulation of a global regulator can identify novel metabolite genes. | 2006 | 16426964 |
| genomic mining for aspergillus natural products. | the genus aspergillus is renowned for its ability to produce a myriad of bioactive secondary metabolites. although the propensity of biosynthetic genes to form contiguous clusters greatly facilitates assignment of putative secondary metabolite genes in the completed aspergillus genomes, such analysis cannot predict gene expression and, ultimately, product formation. to circumvent this deficiency, we have examined aspergillus nidulans microarrays for expressed secondary metabolite gene clusters b ... | 2006 | 16426969 |
| evaluation of agrobacterium-mediated transformation of agaricus bisporus using a range of promoters linked to hygromycin resistance. | there is interest in establishing genetic modification technologies for the cultivated mushroom agaricus bisporus, both for improved crop characteristics and for molecular pharming. for these methods to be successful, it is necessary to establish a set of transformation systems that include robust and reliable vectors for gene manipulation. in this article, we report the evaluation of a series of promoters for driving expression of the escherichia coli hph gene encoding hygromycin phosphotransfe ... | 2006 | 16444014 |
| glass-fiber disks provide suitable medium to study polyol production and gene expression in eurotium rubrum. | eurotium species often dominate the fungal population in stored grain and are responsible for spoilage. in this study we tested the usefulness of glass fiber disks to aid the analysis of growth, polyol content and gene expression in e. rubrum in response to various water activities. growth measurements based on ergosterol content and conidial production indicated that e. rubrum grew as well at 0.86 aw as 0.98 aw. the rate of growth was considerably reduced at 0.83 aw and 0.78 aw. in contrast, un ... | 2005 | 16457343 |
| the aflatoxin biosynthesis cluster gene, aflx, encodes an oxidoreductase involved in conversion of versicolorin a to demethylsterigmatocystin. | biosynthesis of the toxic and carcinogenic aflatoxins by the fungus aspergillus flavus is a complicated process involving more that 27 enzymes and regulatory factors encoded by a clustered group of genes. previous studies found that three enzymes, encoded by vera, ver-1, and afly, are required for conversion of versicolorin a (va), to demethylsterigmatocystin. we now show that a fourth enzyme, encoded by the previously uncharacterized gene, aflx (ordb), is also required for this conversion. a ho ... | 2006 | 16461654 |
| a novel-type substrate-selectivity filter and er-exit determinants in the uapa purine transporter. | we present a functional analysis of the last alpha-helical transmembrane segment (tms12) of uapa, a uric acid-xanthine/h+ symporter in aspergillus nidulans and member of the nucleobase-ascorbate transporter (nat) family. first, we performed a systematic mutational analysis of residue f528, located in the middle of tms12, which was known to be critical for uapa specificity. substitution of f528 with non-aromatic amino acid residues (ala, thr, ser, gln, asn) did not affect significantly the kineti ... | 2006 | 16464466 |
| the aspergillus nidulans xprg (phog) gene encodes a putative transcriptional activator involved in the response to nutrient limitation. | the aspergillus nidulans xprg gene is involved in the regulation of extracellular proteases. a plasmid which complemented the xprg2 mutation was shown to carry the phog gene, reported to encode an acid phosphatase. two phogdelta mutants were constructed and were identical in phenotype to an xprg2 mutant. null mutants were unable to use protein as a carbon or nitrogen source, have lost a repressible acid phosphatase and have pale conidial color. xprg shows similarity to the ndt80 transcriptional ... | 2006 | 16464624 |
| intrinsic in vitro susceptibility of primary clinical isolates of aspergillus fumigatus, aspergillus terreus, aspergillus nidulans, candida albicans and candida lusitaniae against amphotericin b. | a total of 60 clinical fungal isolates from patients without prior amphotericin b treatment and three control strains were evaluated for their intrinsic susceptibility to amphotericin b (amb) using microdilution, etest and disc diffusion assays, on three media each, roswell park memorial institute (rpmi) 1640, antibiotic medium 3 (am3) and high resolution medium. the fungal strains included isolates of aspergillus fumigatus (n = 10), aspergillus terreus (n = 12), aspergillus nidulans (n = 9), ca ... | 2006 | 16466441 |
| clip-170 homologue and nude play overlapping roles in nudf localization in aspergillus nidulans. | proteins in the cytoplasmic dynein pathway accumulate at the microtubule plus end, giving the appearance of comets when observed in live cells. the targeting mechanism for nudf (lis1/pac1) of aspergillus nidulans, a key component of the dynein pathway, has not been clear. previous studies have demonstrated physical interactions of nudf/lis1/pac1 with both nude/nudel/ndl1 and clip-170/bik1. here, we have identified the a. nidulans clip-170 homologue, clipa. the clipa deletion did not cause an obv ... | 2006 | 16467375 |
| differential expression of aspergillus nidulans ammonium permease genes is regulated by gata transcription factor area. | the movement of ammonium across biological membranes is mediated in both prokaryotes and eukaryotes by ammonium transport proteins (amt/mep) that constitute a family of related sequences. we have previously identified two ammonium permeases in aspergillus nidulans, encoded by the meaa and mepa genes. here we show that meaa is expressed in the presence of ammonium, consistent with the function of meaa as the main ammonium transporter required for optimal growth on ammonium as a nitrogen source. i ... | 2006 | 16467464 |
| the phosducin-like protein phna is required for gbetagamma-mediated signaling for vegetative growth, developmental control, and toxin biosynthesis in aspergillus nidulans. | phosducin or phosducin-like protein (phlp) is a positive regulator of gbetagamma activity. the gbeta (sfad) and ggamma (gpga) subunits function in vegetative growth and developmental control in the model filamentous fungus aspergillus nidulans. to better understand the nature of gbetagamma-mediated signaling, phna, encoding an a. nidulans phlp, has been studied. deletion of phna resulted in phenotypes almost identical to those caused by deletion of sfad, i.e., reduced biomass, asexual sporulatio ... | 2006 | 16467480 |
| aspergillus nidulans class v and vi chitin synthases csma and csmb, each with a myosin motor-like domain, perform compensatory functions that are essential for hyphal tip growth. | the polarized synthesis of cell wall components such as chitin is essential for the hyphal tip growth of filamentous fungi. the actin cytoskeleton is known to play important roles in the determination of hyphal polarity in aspergillus nidulans. previously, we suggested that csma, a chitin synthase with a myosin motor-like domain (mmd), was involved in polarized chitin synthesis in a manner dependent on the interaction between the mmd and the actin cytoskeleton. the genome database indicates that ... | 2006 | 16468983 |
| purification and properties of an extracellular endo-1,4-beta-xylanase from penicillium citrinum and characterization of the encoding gene. | an extracellular endo-1,4-beta-xylanase was purified from the culture filtrate of a filamentous fungus penicillium citrinum ferm p-15944 grown on birch-wood xylan. the purified enzyme showed a single band on sds-page with an apparent m(r) of 20,000 and had an isoelectric point below 3.5. xylanase activity was optimal at ph 5.0 and 55 degrees c. the genomic dna and cdnas encoding this protein were cloned and sequenced. southern blot analysis indicated that the xylanase gene (xyna) was present as ... | 2005 | 16473771 |
| impact of fungal drug transporters on fungicide sensitivity, multidrug resistance and virulence. | drug transporters are membrane proteins that provide protection for organisms against natural toxic products and fungicides. in plant pathogens, drug transporters function in baseline sensitivity to fungicides, multidrug resistance (mdr) and virulence on host plants. this paper describes drug transporters of the filamentous fungi aspergillus nidulans (eidam) winter, botrytis cinerea pers and mycosphaerella graminicola (fückel) schroter that function in fungicide sensitivity and resistance. the f ... | 2006 | 16475240 |
| mapping the primary structure of copper/topaquinone-containing methylamine oxidase from aspergillus niger. | the amino acid sequence of methylamine oxidase (meao) from the fungus aspergillus niger was analyzed using mass spectrometry (ms). first, meao was characterized by an accurate molar mass of 72.4 kda of the monomer measured using maldi-tof-ms and by a pi value of 5.8 determined by isoelectric focusing. maldi-tof-ms revealed a clear peptide mass fingerprint after tryptic digestion, which did not provide any relevant hit when searched against a nonredundant protein database and was different from t ... | 2005 | 16475499 |
| agrobacterium tumefaciens-mediated transformation of mucor circinelloides. | the agrobacterium tumefaciens-mediated transformation of the zygomycetous fungus mucor circinelloides is described. a method was also developed for the hygromycin b-based selection of mucor transformants. transformation with the hygromycin b phosphotransferase gene of escherichia coli controlled by the heterologous aspergillus nidulans trpc promoter resulted in hygromycin b-resistant clones. the presence of the hygromycin resistance gene in the genome of the transformants was verified by polymer ... | 2005 | 16475501 |
| genomics of aspergillus fumigatus. | aspergillus fumigatus is a filamentous fungal saprophyte that is ubiquitous in the environment. it is also a human pathogen and induces allergenic response, negatively impacting health care and associated costs significantly around the world. much of the basic biology of this organism is only poorly understood, but the recent completion and publication of its genome sequence provides an excellent tool for researchers to gain insight into these processes. in this review we will summarize some of ... | 2005 | 16499415 |
| nonribosomal peptide synthesis in schizosaccharomyces pombe and the architectures of ferrichrome-type siderophore synthetases in fungi. | a nonribosomal peptide synthetase (nrps) in schizosaccharomyces pombe, which possesses an unusual structure incorporating three adenylation domains, six thiolation domains and six condensation domains, has been shown to produce the cyclohexapeptide siderophore ferrichrome. one of the adenylation domains is truncated and contains a distorted key motif. substrate-binding specificities of the remaining two domains were assigned by molecular modelling to glycine and to n-acetyl-n-hydroxy-l-ornithine ... | 2006 | 16502473 |
| common features and interesting differences in transcriptional responses to secretion stress in the fungi trichoderma reesei and saccharomyces cerevisiae. | secretion stress is caused by compromised folding, modification or transport of proteins in the secretory pathway. in fungi, induction of genes in response to secretion stress is mediated mainly by the unfolded protein response (upr) pathway. this study aims at uncovering transcriptional responses occurring in the filamentous fungi trichoderma reesei exposed to secretion stress and comparing these to those found in the yeast saccharomyces cerevisiae. | 2006 | 16504068 |
| induction of temperate cyanophage as-1 by heavy metal--copper. | it has been reported that some marine cyanophage are temperate and can be induced from a lysogenic phase to a lytic phase by different agents such as heavy metals. however, to date no significant reports have focused on the temperate nature of freshwater cyanophage/cyanobacteria. previous experiments with cyanophage as-1 and cyanobacteria anacystis nidulans have provided some evidence that as-1 may have a lysogenic life cycle in addition to the characterized lytic cycle. | 2006 | 16504132 |
| depletion of aspergillus nidulans cota causes a severe polarity defect which is not suppressed by the nuclear migration mutation nuda2. | the aspergillus nidulans homologue of neurospora crassa cot-1, cota, encoding a member of the ndr protein kinase family, has been cloned and expressed under the control of the conditional alca promoter. depletion of cota by repression of the alca promoter led to a severe growth defect accompanied by loss of polarity. germlings show greatly enlarged volume of the spores and hyphae, accompanied by an increase in number of nuclei per compartment, though the nucleus/volume ratio is not significantly ... | 2006 | 16506053 |
| functional characterization of the putative aspergillus nidulans poly(adp-ribose) polymerase homolog prpa. | poly(adp-ribose) polymerase (parp) is a highly conserved enzyme involved in multiple aspects of animal and plant cell physiology. for example, parp is thought to be intimately involved in the early signaling events that trigger the dna damage response. however, the genetic dissection of parp function has been hindered by the presence of multiple homologs in most animal and plant species. here, we present the first functional characterization of a putative parp homolog (prpa) in a microbial syste ... | 2006 | 16510786 |
| the product of the shr3 orthologue of aspergillus nidulans has restricted range of amino acid transporter targets. | the shra gene of aspergillus nidulans codes for a structural and functional homologue of shr3p, a yeast er membrane protein, which plays a crucial role in the secretory pathway of yeast amino acid permeases. shra is a single-copy gene, whose expression is early activated during germination of a. nidulans conidiospores. shra is localized in the er of the fungal cells and partially complements the shr3delta phenotype. differently from saccharomyces cerevisiae, where shr3p is necessary for membrane ... | 2006 | 16531082 |
| functional analysis of alcs, a gene of the alc cluster in aspergillus nidulans. | the ethanol utilization pathway (alc system) of aspergillus nidulans requires two structural genes, alca and alda, which encode the two enzymes (alcohol dehydrogenase and aldehyde dehydrogenase, respectively) allowing conversion of ethanol into acetate via acetyldehyde, and a regulatory gene, alcr, encoding the pathway-specific autoregulated transcriptional activator. the alcr and alca genes are clustered with three other genes that are also positively regulated by alcr, although they are dispen ... | 2006 | 16531087 |
| combined expression of aspergillus nidulans endoxylanase x24 and aspergillus oryzae (alpha)-amylase in industrial baker's yeasts and their use in bread making. | the aspergillus nidulans endoxylanase x24 and the aspergillus oryzae (alpha)-amylase cdnas were placed under the control of the saccharomyces cerevisiae actin promoter (pact1) and introduced into baker's yeast. bread made with transformants expressing both enzymes (yepact-amy-act-x24) showed a 30% increase in volume and reduced firmness in comparison with that produced with a commercial strain. endoxylanase x24 and (alpha)-amylase seem to act synergistically to improve the quality of bread in te ... | 1996 | 16535419 |
| g-protein and camp-mediated signaling in aspergilli: a genomic perspective. | we have carried out an in silico exploration of the genomes of aspergillus nidulans, aspergillus fumigatus, and aspergillus oryzae, and identified components of g-protein/camp-mediated signaling. putative g-protein coupled receptors (gpcrs) were distributed over nine classes. the gpcrs within classes were well conserved among aspergilli but varied in other ascomycetes. as previously observed in a. nidulans and other fungi, three galpha, one gbeta, and one ggamma subunits of g proteins were ident ... | 2006 | 16546420 |
| [recombinant aspergillus niger glucose oxidase expressed in trichoderma reesei]. | it was expected that recombinant aspergillus niger glucose oxidase could be expressed in trichoderma reesei with stable activity. t. reesei cbhi promoter--cbhi ss. gene--a. niger glucose oxidase gene--t. reesei cbhi terminator--a. nidulans gpd promoter--e. coli hygromycin b phosphotransferase gene--a. nidulans trpc terminator--puc19 (pcbhgod) vector was constructed in e. coli dh5alpha by pcr application and gene cloning methods. t. reesei qm9414 protoplast was transformed by t. reesei cbhi promo ... | 2006 | 16572845 |
| molecular cloning and sequence analysis of the cyanobacterial gene for the large subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase. | ribulose-1,5-bisphosphate carboxylase/oxygenase consists of large subunits (ls) and small subunits. in plants, the ls is encoded in chloroplast dna and the small subunit, in nuclear dna. in cyanobacteria, both subunits are thought to be encoded in chromosomal dna because of prokaryotes. the gene for the ls of ribulose-1,5-bisphosphate carboxylase/oxygenase from a cyanobacterium, anacystis nidulans 6301, has been cloned in pbr322 and subjected to sequence analysis. the coding region contains 1,41 ... | 1983 | 16593333 |
| a cosmid for selecting genes by complementation in aspergillus nidulans: selection of the developmentally regulated ya locus. | we constructed a 9.9-kilobase cloning vector, designated pkby2, for isolating genes by complementation of mutations in aspergillus nidulans. pkby2 contains the bacteriophage lambda cos site, to permit in vitro assembly of phage particles; a bacterial origin of replication and genes for resistance to ampicillin and chloramphenicol, to permit propagation in escherichia coli; the a. nidulans trpc(+) gene, to permit selection in aspergillus; and a unique bamhi restriction site, to permit insertion o ... | 1985 | 16593541 |
| genetic transformation of the fungal pathogen responsible for rice blast disease. | the analysis of complex genetic determinants that control the ability of a fungus to colonize its host has been impaired by the lack of sophisticated genetic tools for characterizing important pathogens. we have developed a system for the genetic transformation of magnaporthe grisea, the causal agent of rice blast disease, to overcome this limitation. a m. grisea arginine auxotroph was shown to contain a mutation (arg3-12) that abolishes ornithine carbamoyltransferase activity. m. grisea strains ... | 1987 | 16593854 |
| protein kinase c (pkca) of aspergillus nidulans is involved in penicillin production. | the biosynthesis of the beta-lactam antibiotic penicillin in the filamentous fungus aspergillus nidulans is catalyzed by three enzymes that are encoded by the acva, ipna, and aata genes. a variety of cis-acting dna elements and regulatory factors form a complex regulatory network controlling these beta-lactam biosynthesis genes. regulators involved include the ccaat-binding complex ancf and anbh1. anbh1 acts as a repressor of the penicillin biosynthesis gene aata. until now, however, little info ... | 2006 | 16598003 |
| antimicrobial, wound healing and antioxidant activity of plagiochasma appendiculatum lehm. et lind. | plagiochasma appendiculatum (aytoniaceae) of the order marchantiales is widely used in the form of paste ethnomedicinally by gaddi tribe in kangra valley for treating skin diseases. in this context, antimicrobical potential of plagiochasma appendiculatum against a wide range of microorganisms was studied. to validate the ethnotherapeutic claims of the plant in skin diseases, wound healing activity was studied, besides antioxidant activity to understand the mechanism of wound healing activity. th ... | 2006 | 16600543 |
| microscopy reveals disease control through novel effects on fungal development: a case study with an early-generation benzophenone fungicide. | the benzophenones are a new class of agricultural fungicides that demonstrate protectant, curative and eradicative/antisporulant activity against powdery mildews. the chemistry is represented in the marketplace by the fungicide metrafenone, recently introduced by basf and discussed in the following paper. the benzophenones show no evidence of acting by previously identified biochemical mechanisms, nor do they show cross-resistance with existing fungicides. the value of microscopy in elucidating ... | 2006 | 16602068 |
| isolation of mutations that bypass the requirement of the septation initiation network for septum formation and conidiation in aspergillus nidulans. | the kinase cascade of the septation initiation network (sin), first revealed in fission yeast, activates the contraction of the actomyosin ring, and plays an essential role in fungal septation. mob1p, an evolutionarily conserved sin protein, is associated with the most downstream kinase of this cascade in fission yeast. in this study, the moba gene encoding a homologous protein was isolated from the filamentous fungus aspergillus nidulans, whose mycelium is made of multinucleate cells. the moba ... | 2006 | 16624915 |
| the region in a subunit of the aspergillus ccaat-binding protein similar to the hap4p-recruiting domain of saccharomyces cerevisiae hap5p is not essential for transcriptional enhancement. | the ccaat-binding complex in aspergillus species, known as the hap complex, consists of at least three subunits, hapb, hapc, and hape. each hap subunit contains an evolutionarily conserved core domain. in this study, a series of the truncated gene, which encodes the hape subunit of aspergillus oryzae, was constructed to survey the regions essential for the transcriptional enhancement of fungal genes. it was revealed that the non-conserved regions and the conserved region similar to the hap4p rec ... | 2006 | 16636442 |
| action spectra for photosystems i and ii in formaldehyde fixed algae. | action spectra were obtained for photosystems i and ii in chemically fixed algal cells and for photosystem i in unfixed lysozyme treated cells. untreated algal cells yielded neither of the 2 light reactions with the reaction mixtures used. the action spectra for photosystem i in the blue-green alga anacystis nidulans and red alga porphyridium cruentum follow the absorption spectrum of chlorophyll a with a small peak in the region of the accessory pigments. in the green alga chlorella pyrenoidosa ... | 1969 | 16657098 |
| regulation of sulfate transport in filamentous fungi. | inorganic sulfate enters the mycelia of aspergillus nidulans, penicillium chrysogenum, and penicillium notatum by a temperature-, energy-, ph-, ionic strength-, and concentration-dependent transport system ("permease"). transport is unidirectional. in the presence of excess external sulfate, atp sulfurylase-negative mutants will accumulate inorganic sulfate intracellularly to a level of about 0.04 m. the intracellular sulfate can be retained against a concentration gradient. retention is not ene ... | 1970 | 16657536 |
| metabolism of delta-aminolevulinic acid in red and blue-green algae. | delta-aminolevulinic acid was incorporated in vivo into c-phycocyanin and b-phycoerythrin in two species of the rhodophyta (cyanidium caldarium, porphyridium cruentum) and three species of the cyanophyta (anacystis nidulans, plectonema boryanum, phormidium luridum). amino acid analysis of phycocyanin-(14)c from c. caldarium cells which had been incubated with delta-aminolevulinate-4-(14)c showed that 84% of the radioactivity incorporated was present in the phycocyanobilin chromophore and less th ... | 1975 | 16659103 |
| light harvesting in anacystis nidulans studied in pigment mutants. | spontaneous pigment mutants of anacystis nidulans were self-selected for improved growth in far red light (> 650 nanometers). questions were asked about those features of the light-harvesting mechanism which altered to give the mutants improved photosynthetic performance in far red. answers were sought by comparing pigment and reaction center concentrations for the parent and six mutants grown in gold fluorescent and in far red light. three significant results emerged. the ratio of reaction cent ... | 1980 | 16661593 |
| chilling susceptibility of the blue-green alga anacystis nidulans: ii. stimulation of the passive permeability of cytoplasmic membrane at chilling temperatures. | potassium ions and amino acids were found to leak from the cytoplasm to the outer medium when the blue-green alga, anacystis nidulans, was exposed to the chilling temperatures. the leakage was marked below the critical temperature regions, the midpoint values for which were around 5 and 14 c in cells grown at 28 and 38 c, respectively. these temperature regions coincided with those critical for the susceptibility of the photosynthetic activities and the carotenoid absorption spectrum previously ... | 1981 | 16661623 |
| chilling-susceptibility of the blue-green alga anacystis nidulans: iii. lipid phase of cytoplasmic membrane. | the lipid phase of cytoplasmic membrane was studied by freeze-fracture electron microscopy in the chilling-susceptible blue-green alga, anacystis nidulans. at growth temperatures, intramembrane particles were distributed at random in the fracture faces of cytoplasmic membrane, whereas, at chilling temperatures, the fracture faces were composed of particle-free and particle-containing regions. these findings indicate that lipids of the cytoplasmic membrane were in the liquid-crystalline state at ... | 1982 | 16662143 |
| photochemical apparatus organization in anacystis nidulans (cyanophyceae) : effect of co(2) concentration during cell growth. | anacystis nidulans cells grown under high (3%) co(2) partial pressure have greater phycocyanin to chlorophyll ratio (phc/chl) relative to cells grown under low (0.2%) co(2) tension (eley (1971) plant cell physiol 12: 311-316). absorbance difference spectrophotometry of a. nidulans thylakoid membranes in the ultraviolet (deltaa(320)) and red (deltaa(700)) regions of the spectrum reveal photosystem ii/photosystem i (psii/psi) reaction center ratio (rcii/rci) changes that parallel those of phc/chl. ... | 1984 | 16663387 |
| characterization of the proton-translocating cytochrome c oxidase activity in the plasma membrane of intact anacystis nidulans spheroplasts. | intact spheroplasts of the cyanobacterium (blue-green alga) anacystis nidulans oxidized various exogenous c-type cytochromes with concomitant outward proton translocation while exogenous ferricytochrome c was not reduced. the h(+)/e(-) stoichiometry was close to 1 with each of the cytochromes and did not depend on the actual rate of the oxidase reaction. observed proton ejections were abolished by the uncoupler carbonyl cyanide m-chlorophenylhydrazone. cyanide, azide, and carbon monoxide inhibit ... | 1984 | 16663770 |
| anacystis nidulans demonstrates a photosystem ii cation requirement satisfied only by ca or na. | anacystis nidulans exhibits a total loss of photosystem ii (psii) activity upon incubation in a nutrient medium deficient in ca(2+) and na(+) and containing a divalent cation chelator. this loss of activity is light-dependent, which corresponds to an energy requirement. likewise, ca(2+) efflux takes place only in cells incubated in light. the loss of psii activity is reversible by addition of submillimolar amounts of either ca(2+) or na(+) to the external medium but not by the addition of any ot ... | 1985 | 16664449 |
| photoinhibition and reactivation of photosynthesis in the cyanobacterium anacystis nidulans. | the susceptibility of photosynthesis to photoinhibition and its recovery were studied on cultures of the cyanobacterium anacystis nidulans. oxygen evolution and low temperature fluorescence kinetics were measured. upon exposure to high light a. nidulans showed a rapid decrease in oxygen evolution followed by a quasi steady state rate of photosynthesis. this quasi steady state rate decreased with increasing photon flux density of the photoinhibitory light. reactivation of photosynthesis in dim li ... | 1985 | 16664559 |
| biosynthesis of a 42-kd polypeptide in the cytoplasmic membrane of the cyanobacterium anacystis nidulans strain r2 during adaptation to low co(2) concentration. | when cells of anacystis nidulans strain r2 grown under high co(2) conditions (3%) were transferred to low co(2) conditions (0.05%), their ability to accumulate inorganic carbon (c(i)) increased up to 8 times. cytoplasmic membranes (plasmalemma) isolated at various stages of low co(2) adaptation were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. there was a marked increase of a 42-kilodalton polypeptide in the cytoplasmic membrane during adaptation; a linear relationship ... | 1986 | 16664655 |
| the susceptibility of photosynthesis to photoinhibition and the capacity of recovery in high and low light grown cyanobacteria, anacystis nidulans. | the susceptibility of photosynthesis to photoinhibition and the rate of its recovery were studied in the cyanobacterium anacystis nidulans grown at a low (10 micromoles per square meter per second) and a high (120 micromoles per square meter per second) photosynthetically active radiation. the rate of light limited photosynthetic o(2) evolution was measured to determine levels of photoinhibition and rates of recovery. studies of photoinhibition and recovery with and without the translation inhib ... | 1987 | 16665264 |
| action spectra for nitrate and nitrite assimilation in blue-green algae. | action spectra for the assimilation of nitrate and nitrite have been obtained for several blue-green algae (cyanobacteria) with different accessory pigment composition. the action spectra for both nitrate and nitrite utilization by nitrate-grown anacystis nidulans l-1402-1 cells exhibited a clear peak at about 620 nanometers, corresponding to photosystem ii (psii) c-phycocyanin absorption, the contribution of chlorophyll a (chl a) being barely detectable. the action spectrum for nitrate reductio ... | 1988 | 16666041 |
| identification and purification of a derepressible alkaline phosphatase from anacystis nidulans r2. | we have examined the increase in alkaline phosphatase activity in the cyanobacterium anacystis nidulans r2 upon phosphate deprivation. much of the activity is released into the medium when a. nidulans is osmotically shocked, indicating that the enzyme is located either in the periplasmic space or is loosely bound to the cell wall. the polypeptide associated with phosphatase activity has been identified as a single species of m(r) 160,000. several lines of evidence demonstrate that this polypepti ... | 1988 | 16666051 |
| regulatory genes controlling fatty acid catabolism and peroxisomal functions in the filamentous fungus aspergillus nidulans. | the catabolism of fatty acids is important in the lifestyle of many fungi, including plant and animal pathogens. this has been investigated in aspergillus nidulans, which can grow on acetate and fatty acids as sources of carbon, resulting in the production of acetyl coenzyme a (coa). acetyl-coa is metabolized via the glyoxalate bypass, located in peroxisomes, enabling gluconeogenesis. acetate induction of enzymes specific for acetate utilization as well as glyoxalate bypass enzymes is via the zn ... | 2006 | 16682457 |
| ambient ph signaling regulates expression of the serine protease gene (spr1) in pine wilt nematode-trapping fungus, monacrosporium megalosporum. | we have cloned and characterized spr1, a putative serine protease gene, from a nematode-trapping fungus, monacrosporium megalosporum. the gene was present as a single copy in the genome. the predicted protein sequence of spr1 is homologous to the putative cuticle-degrading serine proteases pii and azo1 from the nematode-trapping fungus, arthrobotrys oligospora. in the 5' untranslated region near the initiation codon, consensus sequences to an area binding site, a well-known mediator of nitrogen ... | 2008 | 16697631 |
| transcription analysis using high-density micro-arrays of aspergillus nidulans wild-type and crea mutant during growth on glucose or ethanol. | here, we describe how the recently published aspergillus nidulans genome sequence [galagan, j.e., calvo, s.e., cuomo, c., li-jun, m., wortman, j.r., et al., 2005. sequencing of aspergillus nidulans and comparative analysis with a. fumigatus and a. oryzae. nature 438 (7071), 1105-1115] was used to design a high-density oligo array with probes for 3,278 selected genes using the febit geniom one array system. for this purpose, the program oligowiz ii was used to design 24,125 probes to cover the 3, ... | 2006 | 16698295 |
| marker and promoter effects on heterologous expression in aspergillus nidulans. | to study the effects of selection marker, promoter type, and copy number on heterologous expression in aspergillus nidulans, strains were constructed with single- and multicopy plasmid integrations bearing a reporter gene (lacz) under the control of either an inducible (alca) or constitutive (gpda) promoter and one of three aspergillus nutritional marker genes (argb, trpc, or niad). beta-galactosidase activity in the transformants varied over three orders of magnitude, with the majority of level ... | 2006 | 16699756 |
| isolation, characterization, and disruption of dnr1, the area/nit-2-like nitrogen regulatory gene of the zoophilic dermatophyte, microsporum canis. | a homolog of the major nitrogen regulatory genes area from aspergillus nidulans and nit-2 from neurospora crassa was isolated from the zoophilic dermatophyte, microsporum canis. this gene, dnr1, encodes a polypeptide of 761 amino acid residues containing a single zinc-finger dna-binding domain, which is almost identical in amino acid sequence to the zinc-finger domains of area and nit-2. the functional equivalence of dnr1 to area was demonstrated by complementation of an area loss-of-function mu ... | 2006 | 16702104 |
| an extract of morinda citrifolia interferes with the serum-induced formation of filamentous structures in candida albicans and inhibits germination of aspergillus nidulans. | an aqueous extract of morinda citrifolia was shown to interfere with the serum-induced morphological conversion of candida albicans from a cellular yeast to a filamentous form in vitro. the conversion of c. albicans from a cellular yeast to a filamentous form in vivo is associated with pathogenicity. no significant effect on growth in serum-free media was seen at the concentrations used to interfere with the morphological change. the same extract also inhibited the germination of apergillus nidu ... | 2006 | 16710899 |
| transcriptional and bioinformatic analysis of the 56.8 kb dna region amplified in tandem repeats containing the penicillin gene cluster in penicillium chrysogenum. | high penicillin-producing strains of penicillium chrysogenum contain 6-14 copies of the three clustered structural biosynthetic genes, pcbab, pcbc, and pende [barredo, j.l., díez, b., alvarez, e., martín, j.f., 1989. large amplification of a 35-kb dna fragment carrying two penicillin biosynthetic genes in high penicillin producing strains of penicillium chrysogenum. curr. genet. 16, 453-459; smith, d.j., bull, j.h., edwards, j., turner, g., 1989. amplification of the isopenicillin n synthetase g ... | 2006 | 16713314 |
| genomic and experimental evidence for a potential sexual cycle in the pathogenic thermal dimorphic fungus penicillium marneffei. | all meiotic genes (except hop1) and genes encoding putative pheromone processing enzymes, pheromone receptors and pheromone response pathways proteins in aspergillus fumigatus and aspergillus nidulans and a putative mat-1 alpha box mating-type gene were present in the penicillium marneffei genome. a putative mat-2 high-mobility group mating-type gene was amplified from a mat-1 alpha box mating-type gene-negative p. marneffei strain. among 37 p. marneffei patient strains, mat-1 alpha box and mat- ... | 2006 | 16714021 |
| heterotrimeric g protein signaling and rgss in aspergillus nidulans. | heterotrimeric g proteins (g proteins) are conserved in all eukaryotes and are crucial components sensing and relaying external cues into the cells to elicit appropriate physiological and biochemical responses. basic units of the heterotrimeric g protein signaling system include a g protein-coupled receptor (gpcr), a g protein composed of alpha, beta, and gamma subunits, and variety of effectors. sequential sensitization and activation of these g protein elements translates external signals into ... | 2006 | 16728950 |
| the distribution and evolutionary history of the prp8 intein. | we recently described a mini-intein in the prp8 gene of a strain of the basidiomycete cryptococcus neoformans, an important fungal pathogen of humans. this was the second described intein in the nuclear genome of any eukaryote; the first nuclear encoded intein was found in the vma gene of several saccharomycete yeasts. the evolution of eukaryote inteins is not well understood. in this report we describe additional prp8 inteins (bringing the total of these to over 20). we compare and contrast the ... | 2006 | 16737526 |
| a mammalian nudc-like protein essential for dynein stability and cell viability. | cytoplasmic dynein, a minus-end-directed microtubule motor, has been implicated in many fundamental cellular processes; however, little is known regarding the underlying molecular machinery that regulates its stability. in aspergillus nidulans, nuclear distribution gene c (nudc) has been implicated in the regulation of dynein-mediated nuclear migration. here, we characterize a previously undescribed mammalian nudc-like protein (nudcl). the expression and phosphorylation of nudcl are increased du ... | 2006 | 16754861 |
| the fumitremorgin gene cluster of aspergillus fumigatus: identification of a gene encoding brevianamide f synthetase. | a gene encoding a putative dimodular nonribosomal peptide synthetase (nrps) was identified within a gene cluster of aspergillus fumigatus, a species reported to produce fumitremorgins and other prenylated alkaloids. the gene was deleted and overexpressed in the genome reference strain af293, and was also expressed in the naïve host aspergillus nidulans, which lacks the equivalent gene cluster. while neither fumitremorgins nor the dipeptide brevianamide f (cyclo-l-trp-l-pro), an early intermediat ... | 2006 | 16755625 |
| functional characterization of aspergillus nidulans homologues of saccharomyces cerevisiae spa2 and bud6. | the importance of polarized growth for fungi has elicited significant effort directed at better understanding underlying mechanisms of polarization, with a focus on yeast systems. at sites of tip growth, multiple protein complexes assemble and coordinate to ensure that incoming building material reaches the appropriate destination sites, and polarized growth is maintained. one of these complexes is the polarisome that consists of spa2, bud6, pea2, and bni1 in saccharomyces cerevisiae. filamentou ... | 2006 | 16757736 |
| [diagnosis and treatment of aspergillosis in the patients with chronic granulomatous disease]. | chronic granulomatous disease is a rare defect of phagocytosis. increased susceptibility to infections is limited to catalase positive bacteria and fungi. aspergillus spp was reported as the increased clinical problem and the main cause of the deaths. | 2006 | 16758737 |
| outdoor environmental levels of aspergillus spp. conidia over a wide geographical area. | unfortunately, little information is available on the 'normal' air and water load of aspergillus spores and their seasonal changes. we describe the air and water load of aspergillus spores across the province of madrid (spain). we collected samples of air and water (332 samples of air and 148 of water) from selected points and taps (urban and rural environment) in summer, autumn, winter and spring. temperature, wind speed and humidity at each point were obtained. we collected a total of 369 aspe ... | 2006 | 16772229 |
| the ambient ph response rim pathway in yarrowia lipolytica: identification of ylrim9 and characterization of its role in dimorphism. | yarrowia lipolytica is a dimorphic fungus that secretes either an acidic or an alkaline protease depending on the environmental ph. previous results have indicated that secretion of the alkaline protease is under control of the ph signaling pal/rim pathway originally described in aspergillus nidulans. several y. lipolytica mutants defective in some rim components of this pathway have been previously isolated and the rim genes characterized. in the present study, y. lipolytica rim9 (pali) gene (y ... | 2006 | 16775780 |
| reducing the cost of resistance; experimental evolution in the filamentous fungus aspergillus nidulans. | we have studied compensatory evolution in a fludioxonil resistant mutant of the filamentous fungus aspergillus nidulans. in an evolution experiment lasting for 27 weeks (about 3000 cell cycles) 35 parallel strains of this mutant evolved in three different environmental conditions. our results show a severe cost of resistance (56%) in the absence of fludioxonil and in all conditions the mutant strain was able to restore fitness without loss of the resistance. in several cases, the evolved strain ... | 2006 | 16780512 |
| the zn(ii)2cys6 putative transcription factor nosa controls fruiting body formation in aspergillus nidulans. | the filamentous fungus aspergillus nidulans reproduces asexually with conidiospores and sexually with ascospores, both of which are the result of complex morphogenetic pathways. the developmental decisions for both ways of reproduction largely depend on the action of stage-specific transcription factors. here we have characterized the putative zn(ii)(2)cys(6) transcription factor nosa (number of sexual spores), a protein of 675 aa, which shares 44% sequence identity to pro1 from sordaria macrosp ... | 2006 | 16780567 |
| consecutive gene deletions in aspergillus nidulans: application of the cre/loxp system. | the ability to perform multiple gene deletions is an important tool for conducting functional genomics. we report the development of a sequential gene deletion protocol for the filamentous fungus aspergillus nidulans using the cre/loxp recombinase system of bacteriophage p1. a recyclable genetic marker has been constructed by incorporating loxp direct repeats either side of the neurospora crassa pyr-4 gene (encodes orotidine 5'-monophosphate decarboxylase) which is able to complement the a. nidu ... | 2006 | 16783565 |
| chemical characterization and quantification of siderophores produced by marine and terrestrial aspergilli. | ten aspergilli (five each from marine and terrestrial habitats) were screened for siderophore production. all test isolates produced siderophores as indicated by a positive reaction in the fecl(3) test, chrome azurol sulphonate assay, and chrome azurol sulphonate agar plate test. further, the test isolates were compared for their siderophore production potential and chemical characteristics. examination of the chemical nature of the siderophores revealed that all test isolates produced hydroxama ... | 2006 | 16788730 |