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a snake-like serine proteinase (pmsnake) activates prophenoloxidase-activating system in black tiger shrimp penaeus monodon.clip domain serine proteinases (clipsps) play critical roles in the activation of proteolytic cascade in invertebrate immune systems including the prophenoloxidase (propo) activating system. in this study, we characterized a snake-like serine protease, namely pmsnake, from the shrimp penaeus monodon which has previously been identified based on the subtractive cdna library of propo double-stranded rna (dsrna)-treated hemocytes. an open reading frame of pmsnake contains 1068 bp encoding a predict ...201727693192
first report of aedes (stegomyia) albopictus (diptera: culicidae) in oran, west of algeria.the increasing globalisation of trades, human movements and environmental changes facilitate the introduction and the establishment of the invasive asian tiger mosquito aedes albopictus outside its native geographical area. alerted by the complaints about mosquito biting which occurred daytime for the inhabitants of the seaside town ain turk (west of algeria), an entomological survey was conducted in december 2015 to determine the origin of this nuisance. among the collected mosquitoes, specimen ...201627697483
molecular cloning and functional characterization of cyclin e and cdk2 from penaeus monodon.reduced reproductive performance of the black tiger shrimp (penaeus monodon) has caused economic losses and hampered the fishing industry. detailed investigation of the molecular mechanism by which the cell cycle is regulated in this organism is needed to understand the development and maturation of ovaries and oocytes, with a view to improving reproductive capacity. cell cycle progression is mainly determined by cyclin-dependent kinase (cdk) and cyclin complexes, the cyclin e/cdk2 complex playi ...201627706738
density and carrying capacity in the forgotten tigerland: tigers in understudied nepalese churia.while there are numerous wildlife ecology studies in lowland areas of nepal, there are no in-depth studies of the hilly churia habitat even though it comprises 7,642 km(2) of potential wildlife habitat across the terai arc. we investigated tiger, leopard, and prey densities across this understudied habitat. our camera trapping survey covered 536 km(2) of churia and surrounding areas within chitwan national park (cnp). we used 161 trapping locations and accumulated 2,097 trap nights in a 60-day s ...201627734629
and in other news.tigers? rhinos? sloths? public perceptions of saga holidays may have to be reconsidered if michael o'flaherty's experience is typical.200827736600
process optimization for high-pressure processing of black tiger shrimp ( penaeus monodon) using response surface methodology.this study aims to investigate the effect of high-pressure processing on the quality of black tiger shrimp using response surface methodology. a central composite rotatable design was applied to evaluate the effects of three processing parameters, namely pressure (300-600 mpa), temperature (30-50 ℃), and time (0-15 min), on the inactivation rate of staphylococcus aureus and physical properties (color and texture) of shrimp and to optimize the process conditions to achieve maximum bacterial inact ...201727758875
epidemiology of la crosse virus emergence, appalachia region, united states.la crosse encephalitis is a viral disease that has emerged in new locations across the appalachian region of the united states. conventional wisdom suggests that ongoing emergence of la crosse virus (lacv) could stem from the invasive asian tiger (aedes albopictus) mosquito. efforts to prove this, however, are complicated by the numerous transmission routes and species interactions involved in lacv dynamics. to analyze lacv transmission by asian tiger mosquitoes, we constructed epidemiologic mod ...027767009
first record of stegomyia albopicta (= aedes albopictus) in morocco: a major threat to public health in north africa?the asian tiger mosquito stegomyia albopicta (= aedes albopictus) (diptera: culicidae), native to asian forests, is a nuisance mosquito and is responsible for the transmission of arboviruses of public health importance, such as dengue, chikungunya and zika viruses. it has colonized parts of all continents, except antarctica, over the past 30-40 years. however, to date, the only records of s. albopicta in north africa refer to occasional collections in 2010 and 2014 in algeria. in early september ...201727775162
cadmium-induced apoptosis of siberian tiger fibroblasts via disrupted intracellular homeostasis.heavy metals can cause great harm to siberian tigers in the natural environment. cadmium (cd(2+)) is an environmental contaminant that affects multiple cellular processes, including cell proliferation, differentiation, and survival. it has been shown to induce apoptosis in a variety of cell types and tissues.201627776532
safety and efficacy of using a single transradial mac guiding catheter for coronary angiography and intervention in patients with st elevation myocardial infarction.there are limited data on the impact of using a single dedicated radial guiding catheter in primary percutaneous coronary intervention (pci) via radial access.201727781297
new developments in the management of non-small-cell lung cancer, focus on rociletinib: what went wrong?recently, the development of the third-generation epidermal growth factor receptor-small molecule inhibitor (egfr-tki) rociletinib had failed. in this review, the wide-ranging aspects of the evolution of egfr-tkis were collected, with a special focus on rociletinib. the influence of different oncogenic mutations on egfr activity was also discussed. resistance to the first (erlotinib, gefitinib)- and second (afatinib)-generation egfr-tkis provided the rationale behind the development of the third ...201627785053
effect of enzyme pre-treatments on bioactive compounds in extracted tiger nut oil and sugars in residual meals.tiger nut oil is a novel oil that requires more research data on its characteristics. in this study, the oil was extracted using both enzyme-aided pressing (eap) and aqueous enzymatic extraction (aee) methods. using enzymes as a pre-treatment prior to mechanical pressing increased the concentration of some phenolic acids and tocopherols present in extracted oils compared to controls. high pressure processing as a pre-treatment before aqueous enzymatic extraction also enhanced tocopherols and tot ...201627795569
[volvulus of the small bowel due to ascaris lumbricoides package: about a case].we report an exceptional case of a 7 year-old patient with necrotic small bowel volvulus due to adult ascaris lumbricoides. at the admission, the child had intestinal obstruction evolving since two days with alteration of general state. abdominal radiography without preparation showed small bowel air-fluid levels and tiger-stripe appearance evoking the diagnosis of acute intestinal obstruction associated with abdominal mass. after resuscitation, the surgical treatment consisted of laparotomy whi ...201627795803
first record of the asian tiger mosquito aedes albopictus in hidalgo state, mexico.the occurrence of the asian tiger mosquito, aedes albopictus , has been reported in the mexican states of tamaulipas, nuevo león, coahuila (northeastern), veracruz, chiapas, quintana roo (southeastern), morelos, san luis potosí (middle), and sinaloa (northwestern). in april and september 2012, ae. albopictus was collected in a variety of habitats and landing/biting on the collecting personnel in 12 counties of hidalgo state (middle). this is the first record of the occurrence of this species in ...201627802405
comparison of carnivore, omnivore, and herbivore mammalian genomes with a new leopard assembly.there are three main dietary groups in mammals: carnivores, omnivores, and herbivores. currently, there is limited comparative genomics insight into the evolution of dietary specializations in mammals. due to recent advances in sequencing technologies, we were able to perform in-depth whole genome analyses of representatives of these three dietary groups.201627802837
aedes albopictus and aedes japonicus - two invasive mosquito species with different temperature niches in europe.aedes albopictus and ae. japonicus are two of the most widespread invasive mosquito species that have recently become established in western europe. both species are associated with the transmission of a number of serious diseases and are projected to continue their spread in europe.201627814747
characterization of molecular properties and regulatory pathways of crustinpm1 and crustinpm7 from the black tiger shrimp penaeus monodon.crustinpm1 and crustinpm7 are the two most abundant isoforms of crustins identified from the hemocytes of the black tiger shrimp, penaeus monodon. crustinpm1 inhibits only gram-positive bacteria, while crustinpm7 acts against both gram-positive and gram-negative bacteria. this work aims to characterize the molecular properties of recombinant crustinpm1 and crustinpm7, and the regulatory pathways of these two crustins. circular dichroism spectroscopy revealed that crustinpm1 contained 40.81% alph ...201727815179
a new species of tiger pleco panaqolus (siluriformes: loricariidae) from the xingu basin, brazil.panaqolus tankei is described from the xingu river, brazil. the new species is diagnosed from p. albomaculatus, p. dentex, p. nix, p. nocturnus, and p. koko by its color pattern consisting of dark and light diagonal bars on the body and bands on the fins (vs. body and fins without bars or bands); from p. albivermis, p. maccus, and p. purusiensis by the width of the dark bars being more or less the same of the light bars (vs. dark bars at least two or three times wider than light bars) and from p ...201627828981
genetic diversity of the giant tiger prawn penaeus monodon in relation to trace metal pollution at the tanzanian coast.the genetic diversity of giant tiger prawns in relation to trace metals (tms) pollution was analysed using 159 individuals from eight sites at the tanzanian coast. the seven microsatellites analysed showed high degree of polymorphism (4-44 alleles). the measured genetic diversity (ho=0.592±0.047) was comparable to that of populations in the western indian ocean. apart from that, correlation analysis revealed significant negative associations between genetic diversity and tms pollution (p<0.05), ...201727829502
methodological approach for the collection and simultaneous estimation of greenhouse gases emission from aquaculture ponds.global warming/climate change is the greatest environmental threat of our time. rapidly developing aquaculture sector is an anthropogenic activity, the contribution of which to global warming is little understood, and estimation of greenhouse gases (ghgs) emission from the aquaculture ponds is a key practice in predicting the impact of aquaculture on global warming. a comprehensive methodology was developed for sampling and simultaneous analysis of ghgs, carbon dioxide (co2), methane (ch4), and ...201627848111
the impacts of hammer-mill screen size and grain particle size on the performance of broiler chickens offered diets based on two red sorghum varieties.1. the two red grain sorghums were extensively characterised. kafirin, polyphenolic compounds, free, conjugated and bound phenolic acids, phytate concentrations and starch pasting profiles were determined. 2. the experiment consisted of a 2 × 4 factorial array of dietary treatments comprising two red sorghum varieties (tiger and block i) ground through 4 hammer-mill screen sizes (2.0, 3.2, 4.8 6.0 mm) prior to incorporation into nutritionally equivalent diets. eight steam-pelleted dietary treatm ...201627848267
spontaneous proliferative and neoplastic lesions in thyroid and parathyroid glands of nondomestic felids.based on microscopic and immunohistochemical characterization, we documented spontaneous proliferative and neoplastic lesions in the thyroid and parathyroid glands of nondomestic felids. ten animals (4 leopards, 3 tigers, and 3 cougars), all with a previous diagnosis of thyroid neoplasia were identified from the university of tennessee college of veterinary medicine database. the mean age of affected animals was 15.9 y. twelve neoplasms were identified; 2 animals had 2 concurrent neoplasms. afte ...201727852814
modelling tooth-prey interactions in sharks: the importance of dynamic testing.the shape of shark teeth varies among species, but traditional testing protocols have revealed no predictive relationship between shark tooth morphology and performance. we developed a dynamic testing device to quantify cutting performance of teeth. we mimicked head-shaking behaviour in feeding large sharks by attaching teeth to the blade of a reciprocating power saw fixed in a custom-built frame. we tested three tooth types at biologically relevant speeds and found differences in tooth cutting ...201627853592
prey depletion as a threat to the world's large carnivores.large terrestrial carnivores are an ecologically important, charismatic and highly endangered group of species. here, we assess the importance of prey depletion as a driver of large carnivore endangerment globally using lists of prey species for each large carnivore compiled from the literature. we consider spatial variation in prey endangerment, changes in endangerment over time and the causes of prey depletion, finding considerable evidence that loss of prey base is a major and wide-ranging th ...201627853599
a conceptual framework for understanding illegal killing of large carnivores.the growing complexity and global nature of wildlife poaching threaten the survival of many species worldwide and are outpacing conservation efforts. here, we reviewed proximal and distal factors, both social and ecological, driving illegal killing or poaching of large carnivores at sites where it can potentially occur. through this review, we developed a conceptual social-ecological system framework that ties together many of the factors influencing large carnivore poaching. unlike most conserv ...201727854069
relationship between voice complaints and subjective and objective measures of vocal function in iranian female teachers.teachers are at high risk of developing voice problems because of the excessive vocal demands necessitated by their profession. teachers' self-assessment of vocal complaints, combined with subjective and objective measures of voice, may enable better therapeutic decision-making. this investigation compared audio-perceptual assessment and acoustic variables in teachers with and without voice complaints.201727865552
environmentally induced tissue responses of hematopoietic system in abu mullet (liza abu) and tiger tooth croaker (otolithes ruber) from the persian gulf.the present investigation aimed to assess the possibility of using plasma levels of erythropoietin (epo) hormone and tissue changes of hematopoietic organs as biomarkers of environmental pollution in abu mullet (liza abu) and tiger tooth croaker (otolithes ruber) collected from musa creek (northwest of the persian gulf). 120 l. abu and o. ruber were collected from five stations at the musa creek: petrochemical, ghanam, doragh, zangi and patil stations. blood samples were obtained from the caudal ...201727866074
modelling spatial and temporal forest cover change patterns (1973-2020): a case study from south western ghats (india).this study used time series remote sensing data from 1973, 1990 and 2004 to assess spatial forest cover change patterns in the kalakad-mundanthurai tiger reserve (kmtr), south western ghats (india). analysis of forest cover changes and its causes are the most challenging areas of landscape ecology, especially due to the absence of temporal ground data and comparable space platform based data. comparing remotely sensed data from three different sources with sensors having different spatial and sp ...200827873862
a novel c-type lectin in the black tiger shrimp penaeus monodon functions as a pattern recognition receptor by binding and causing bacterial agglutination.c-type lectins are pattern recognition proteins that play important roles in innate immunity in invertebrates by mediating the recognition of pathogens. in this study, a novel c-type lectin gene, pmclec, was cloned and characterized from the black tiger shrimp penaeus monodon. the open reading frame of pmclec is 657 bp in length. it encodes a predicted protein of 218 amino acids with a calculated molecular mass and an isoelectric point of 24086 da and 4.67, respectively. sequence analysis of pmc ...201727876622
the invasive asian tiger mosquito aedes albopictus (diptera: culicidae) in germany: local reproduction and overwintering.within the framework of a german mosquito monitoring programme, the 'mueckenatlas' (mosquito atlas) has been established as an instrument of citizen participation in mosquito mapping. in 2015, a strikingly large number of aedes albopictus, which had not been considered established in germany, was submitted. three of six collection sites showed local reproduction, with demonstration of developmental stages over three months at two sites. the third populated site was checked only once in october. ...201727876647
studies on prevalence of endo-parasitic infection in wild carnivores maintained under captive state.the present study was conducted to observe the prevalence of endo-parasites in wild carnivores maintained under captive state at tyavarekoppa tiger and lion safari unit near to veterinary college, shimoga. a total of 54 wild carnivores were included in the study and the fresh fecal samples were collected, examined on the same day by direct and sedimentation techniques for endo-parasitic eggs/ova in the laboratory. out of 15 tigers samples screened, 12 were harboring mixed infections of strongyle ...201627876905
investigation of the chemical markers for experiential quality evaluation of crude aconite by uhplc-q-tof-ms.j. sep. sci. 2016, 39, 4281-4289 doi: 10.1002/jssc.201600567 aconite, called tiger and wolf drug, is known in traditional chinese medicine for its strong cardiac effect. in the past, the toxins extracted from aconite were used as arrow poisons to kill animals, hence the name "wolf's bane." to a certain extent, the wolf represents the medicinal characteristics of aconite: fierce, wild and intractable. the cover picture shows, besides the wolf head as the main element, a flow chart of our study an ...201627886464
influence of pork and pork by-products on macronutrient and energy digestibility and palatability in large exotic felids.two experiments were conducted to evaluate digestibility and palatability of a new commercial pork-based raw diet for zoo-managed felids. currently 2 protein sources (beef or horse) comprise the majority of commercial raw meat diet formulations for exotic carnivores in zoological institutions. pork-based diets have traditionally not been widely utilized and thus nutrient digestibility of pork has not been adequately evaluated in exotic carnivores. the objectives of this study were 1) to determin ...201627898922
the role of latitudinal, genetic and temperature variation in the induction of diapause of papilio glaucus (lepidoptera: papilionidae).a key adaptation in insects for dealing with variable environmental conditions is the ability to diapause. the tiger swallowtail butterflies, papilio glaucus and p. canadensis are ideal species to explore the genetic causes and population genetic consequences of diapause because divergence in this trait is believed to be a salient factor in maintaining a hybrid zone between these species. yet little is known about the factors that influence diapause induction in this system. here we explored how ...201627900827
krasilnikoviella muralis gen. nov., sp. nov., a member of the family promicromonosporaceae, isolated from the takamatsuzuka tumulus stone chamber interior and reclassification of promicromonospora flava as krasilnikoviella flava comb. nov.a gram-stain-positive, facultatively anaerobic actinomycete, designated strain t6220-5-2bt, was isolated from a sample taken from a mouldy spot on the surface of a mural painting (the white tiger, byakko) inside the stone chamber of takamatsuzuka tumulus in asuka village, nara prefecture, japan. based on 16s rrna gene sequence analysis of the isolate, it was closely related to the genus promicromonospora, but formed of a novel lineage within the family promicromonosporaceae. the closest related ...201727902237
conifer monoterpene chemistry during an outbreak enhances consumption and immune response of an eruptive folivore.changes in the chemical composition of plant defense compounds during herbivory can impact herbivore resource allocation patterns and thereby herbivore survival, growth, and immune response against endoparasitoid infection. few studies have investigated folivore responses to changes in plant chemistry that occur under outbreak conditions in mature conifer systems. using data from an earlier observational field study, we carried out laboratory bioassays to test how variation in monoterpenes in pi ...201627909855
molecular cloning, expression and functional analysis of three subunits of protein phosphatase 2a (pp2a) from black tiger shrimps (penaeus monodon).protein phosphatase 2a (pp2a) is a cellular serine-threonine (ser/thr) phosphatase that plays a crucial role in regulating most cellular functions. in the present study, the full-length cdnas of three subunits of pmpp2a (pmpp2a-a, pp2a-b and pp2a-c) were cloned from penaeus monodon, which are the first available for shrimps. sequence analysis showed that pmpp2a-a, pmpp2a-b and pmpp2a-c encoded polypeptides of 591, 443, and 324 amino acids, respectively. the mrnas of three subunits of pmpp2a were ...201727914955
characteristics and fertility of sumatran tiger spermatozoa cryopreserved with different sugars.cryopreservation of semen is one of the most important methods for the preservation of endangered tigers.201727925009
infantile lhermitte-duclos disease treated successfully with rapamycin.lhermitte-duclos disease is a rare hamartomatous tumor of the cerebellum resulting from a mutation in the phosphatase and tensin homolog (pten) gene: it has been reported in fewer than 10 infants. rapamycin treatment has not yet been described in lhermitte-duclos disease. the infant underwent shunt placement shortly after birth for aqueductal stenosis. her clinical progression included failure to thrive, seizures, episodes of decerebrate posturing, loss of respiratory drive, and pituitary insuff ...201727932596
the worldwide spread of the tiger mosquito as revealed by mitogenome haplogroup diversity.in the last 40 years, the asian tiger mosquito aedes albopictus, indigenous to east asia, has colonized every continent except antarctica. its spread is a major public health concern, given that this species is a competent vector for numerous arboviruses, including those causing dengue, chikungunya, west nile, and the recently emerged zika fever. to acquire more information on the ancestral source(s) of adventive populations and the overall diffusion process from its native range, we analyzed th ...201627933090
diversity of cultivable midgut microbiota at different stages of the asian tiger mosquito, aedes albopictus from tezpur, india.aedes aegypti and ae. albopictus are among the most important vectors of arboviral diseases, worldwide. recent studies indicate that diverse midgut microbiota of mosquitoes significantly affect development, digestion, metabolism, and immunity of their hosts. midgut microbiota has also been suggested to modulate the competency of mosquitoes to transmit arboviruses, malaria parasites etc. interestingly, the midgut microbial flora is dynamic and the diversity changes with the development of vectors ...201627941985
hplc-ms analysis of lichen-derived metabolites in the life stages of crambidia cephalica (grote & robinson).tiger moths (lepidoptera: erebidae: arctiinae: arctiini) are notable for their specialized associations with hosts that produce toxic secondary compounds, and are thus an ideal study system for understanding insect-plant interactions and the evolution of antipredatory defense. likewise, their sister lineage (arctiinae: lithosiini) has been documented feeding on algae and lichens, and is known to sequester lichen-derived secondary compounds from the larval to adult stages. prevalence of lichenivo ...201727966190
the seahorse genome and the evolution of its specialized morphology.seahorses have a specialized morphology that includes a toothless tubular mouth, a body covered with bony plates, a male brood pouch, and the absence of caudal and pelvic fins. here we report the sequencing and de novo assembly of the genome of the tiger tail seahorse, hippocampus comes. comparative genomic analysis identifies higher protein and nucleotide evolutionary rates in h. comes compared with other teleost fish genomes. we identified an astacin metalloprotease gene family that has underg ...201627974754
provocative endoscopy: let sleeping dogs lie or grab the tiger by the tail? 201727986105
protection of penaeus monodon against white spot syndrome by continuous oral administration of a low concentration of bacillus subtilis spores expressing the vp28 antigen.in this study, bacillus subtilis spores expressing a chimeric protein, cotb-vp28, were used as a probiotic vaccine to protect black tiger shrimps (penaeus monodon) against white spot syndrome virus (wssv) infection. oral administration of pellets coated with cotb-vp28 spores (at ≥1 × 10(9 ) cfu per g pellet) to shrimps induced immune-relating phenoloxydase activity (po) in shrimps after 14 days of feeding (prior challenge) and at day 3 post challenge (1·26 and 1·70 fold increase respectively). a ...201727992657
beware the tiger-x's stripes: rapid cataract formation in patients taking rociletinib. 201728002880
mosquito species (diptera: culicidae) persistence and synchrony across an urban altitudinal gradient.patterns of mosquito spatial persistence and temporal presence, as well as synchrony, i.e., the degree of concerted fluctuations in abundance, have been scarcely studied at finely grained spatial scales and over altitudinal gradients. here, we present a spatial persistence, temporal presence, and synchrony analysis of four common mosquito species across the altitudinal gradient of mt. konpira in nagasaki, japan. we found that aedes albopictus (skuse) was more frequently found at the mountain bas ...201728025246
the immune response of the c-jun in the black tiger shrimp (penaeus monodon) after bacterial infection.the transcription factor c-jun widely exists in vertebrates and invertebrates and plays an important role in various kinds of stimulus response. in this study, pmc-jun gene was first cloned from penaeus monodon. the full-length cdna of pmc-jun was 1857 bp in length and included an 879 bp open reading frame (orf), which encoded 293 amino acids. qrt-pcr analysis results showed that pmc-jun mrnas were ubiquitously expressed in all the examined tissues. the highest expression level was observed in g ...201728027988
histologic parameters for detecting multiple blood meals in aedes albopictus (skuse) (diptera: culicidae) during a single gonotrophic cycle.the histologic technique was used for its usefulness in detecting multiple blood feedings in aedes albopictus during a single gonotrophic cycle. in a laboratory study, in which 200 blood-engorged ae. albopictus mosquito females during a 3-day period were examined, 69% (138/200) of these females imbibed two blood meals when the interval between meals was from 8 to 48 h and the time between the second blood meal and fixation ranged from 0 to 32 h. at the interval outside this range, only 23% of th ...201628028629
period for normalization of voice acoustic parameters in indian pediatric cochlear implantees.the purpose of this study was to investigate the duration required by children with cochlear implants to approximate the norms of voice acoustic parameters.201728029557
targeting a hidden enemy: pyriproxyfen autodissemination strategy for the control of the container mosquito aedes albopictus in cryptic habitats.the asian tiger mosquito, aedes albopictus, is a vector of dengue, chikungunya, and zika viruses. this mosquito inhabits a wide range of artificial water-holding containers in urban and suburban areas making it difficult to control. we tested the hypothesis that female-driven autodissemination of an insect growth regulator could penetrate cryptic oviposition habitats difficult to treat with conventional insecticidal sprays.201628033379
free breathing whole-heart 3d cine mri with self-gated cartesian trajectory.to present a method that uses a novel free-running self-gated acquisition to achieve isotropic resolution in whole heart 3d cartesian cardiac cine mri.201728034638
an overview of mosquitoes and emerging arboviral infections in the zagreb area, croatia.mosquito control in the zagreb area has been conducted for many years, whereas the fauna has only been investigated in the last 20 years. so far 30 mosquito species have been detected in the city area. culex pipiens form molestus is the dominant mosquito species in indoor breeding sites. in forested areas and areas exposed to flooding, the active period is early spring and the dominant species are ochlerotatus sticticus, ochlerotatus cantans, ochlerotatus geniculatus and aedes vexans. the eudomi ...201628036307
perspectives provided by leopard and other cat genomes: how diet determined the evolutionary history of carnivores, omnivores, and herbivores.recent advances in genome sequencing technologies have enabled humans to generate and investigate the genomes of wild species. this includes the big cat family, such as tigers, lions, and leopards. adding the first high quality leopard genome, we have performed an in-depth comparative analysis to identify the genomic signatures in the evolution of felid to become the top predators on land. our study focused on how the carnivore genomes, as compared to the omnivore or herbivore genomes, shared ev ...201728042784
impacts of canine distemper virus infection on the giant panda population from the perspective of gut microbiota.the recent increase in infectious disease outbreaks has been directly linked to the global loss of biodiversity and the decline of some endangered species populations. between december 2014 and march 2015, five captive giant pandas died due to canine distemper virus (cdv) infection in china. cdv has taken a heavy toll on tigers and lions in recent years. here, we describe the first gut microbiome diversity study of cdv-infected pandas. by investigating the influence of cdv infection on gut bacte ...201728051146
laboratory cohabitation challenge model for shrimp hepatopancreatic microsporidiosis (hpm) caused by enterocytozoon hepatopenaei (ehp).enterocytozoon hepatopenaei (ehp) causes hepatopancreatic microsporidiosis (hpm) in shrimp. it is probably endemic in australasia and was first characterized and named from the giant or black tiger shrimp penaeus monodon from thailand in 2009. later, it was also found to infect exotic penaeus vannamei imported for cultivation in asia. hpm is not normally associated with shrimp mortality, but information from shrimp farmers indicates that it is associated with significant growth retardation that ...201728056950
nanocaged platforms: modification, drug delivery and nanotoxicity. opening synthetic cages to release the tiger.nanocages (ncs) have emerged as a new class of drug-carriers, with a wide range of possibilities in multi-modality medical treatments and theranostics. nanocages can overcome such limitations as high toxicity caused by anti-cancer chemotherapy or by the nanocarrier itself, due to their unique characteristics. these properties consist of: (1) a high loading-capacity (spacious interior); (2) a porous structure (analogous to openings between the bars of the cage); (3) enabling smart release (a key ...201728067384
preliminary considerations about the presence of aedes albopictus (skuse 1897) (diptera: culicidae) during winter in the northwestern italy.aedes albopictus, the asian tiger mosquito, was unintentionally introduced in italy at the beginning of the 1990s. in few decades it spread almost in the whole country. in piedmont, the first report dates back to 1994. usually, temperate populations are affected by seasonal temperature and photoperiodicity and can overwinter by producing eggs that undergo a winter diapause. in rome females of the species extended their trophic activity to the coldest months of the year, but there is no notice ab ...201728067941
optimization of production and quality evaluation of maize-based snack supplemented with soybean and tiger-nut (cyperus esculenta) flour.optimization of the production and evaluation of the quality of maize-based snack supplemented with soy and tigernut flour was carried out. experimental design for the composite flour was carried out using optimal design model of response surface methodology. the variables were toasted maize flour (75-85%), soy flour (10-20%), and tigernut flour (5-10%); while the responses were proximate composition and mineral contents. three blends were selected from optimization results; runs 2, 7, and 11. t ...201728070311
positive preferences: the emotional valence of what an avatar says matters.this study examined preferences and ascriptions of human control for avatars associated with positive and negative language. an avatar is a graphical representation of an individual user in a virtual world. users form judgments of other virtual world users based on the appearance and behavior of their avatars in the absence of physical cues. in particular, users have previously displayed sensitivity toward anthropomorphism and verbal behavior of avatars they encounter within virtual environments ...201728080149
characterization and expression analysis of a chitinase gene (pmchi-4) from black tiger shrimp (penaeus monodon) under pathogen infection and ambient ammonia nitrogen stress.chitinase is a multi-gene family, which play important physiological roles in crustaceans, involved in several biological processes, including digestion, molting and defense against viruses. in the present study, a chitinase-4 gene (pmchi-4) was cloned from penaeus monodon by rapid amplification of cdna ends (race). the full length of pmchi-4 cdna was 2178 bp, including an 1815 bp open reading frame (orf) which encoded 604 amino acid residues. the predicted pmchi-4 protein was 67.7 kda and share ...201728089896
endocranial morphology of the extinct north american lion (panthera atrox).the extinct north american lion (panthera atrox) is one of the largest felids (mammalia, carnivora) to have ever lived, and it is known from a plethora of incredibly well-preserved remains. despite this abundance of material, there has been little research into its endocranial anatomy. ct scans of a skull of p. atrox from the pleistocene la brea tar pits were used to generate the first virtual endocranium for this species and to elucidate previously unknown details of its brain size and gross st ...201628092905
investigation of the tiger milk medicinal mushroom, lignosus rhinocerotis (agaricomycetes), as an antiulcer agent.the aim of this study was to determine the antiulcer activity of lignosus rhinocerotis in rats. a total of 48 sprague-dawley rats were used in ethanol-induced, aspirin-induced, and water immersion-restraint stress-induced ulcer models. rats were equally divided into 4 groups for each model and orally administered 5 ml/kg distilled water, 20 mg/kg omeprazole, as well as 250 and 500 mg/kg of l. rhinocerotis powder. l. rhinocerotis powder at both 250 and 500 mg/kg doses demonstrated significant (p ...201628094747
defining the risk of zika and chikungunya virus transmission in human population centers of the eastern united states.the recent spread of mosquito-transmitted viruses and associated disease to the americas motivates a new, data-driven evaluation of risk in temperate population centers. temperate regions are generally expected to pose low risk for significant mosquito-borne disease; however, the spread of the asian tiger mosquito (aedes albopictus) across densely populated urban areas has established a new landscape of risk. we use a model informed by field data to assess the conditions likely to facilitate loc ...201728095405
sequence analysis of mitochondrial genome of toxascaris leonina from a south china tiger.toxascaris leonina is a common parasitic nematode of wild mammals and has significant impacts on the protection of rare wild animals. to analyze population genetic characteristics of t. leonina from south china tiger, its mitochondrial (mt) genome was sequenced. its complete circular mt genome was 14,277 bp in length, including 12 protein-coding genes, 22 trna genes, 2 rrna genes, and 2 non-coding regions. the nucleotide composition was biased toward a and t. the most common start codon and stop ...201628095667
reconstruction of the cortical maps of the tasmanian tiger and comparison to the tasmanian devil.the last known tasmanian tiger (thylacinus cynocephalus)-aka the thylacine-died in 1936. because its natural behavior was never scientifically documented, we are left to infer aspects of its behavior from museum specimens and historical recollections of bushmen. recent advances in brain imaging have made it possible to scan postmortem specimens of a wide range of animals, even more than a decade old. any thylacine brain, however, would be more than 100 years old. here, we show that it is possibl ...201728099446
why the resistance: minimally invasive pancreaticoduodenectomy-saving the patient from tigers. 201628100937
international evolution of tiger informatics competencies.the tiger initiative aims to explain how to equip practicing nurses with informatics competencies. this chapter describes a collaborative effort to identify global informatics requirements in relation to core competencies and to match them with national and regional needs. recommendations from the tiger informatics competency synthesis project, described here, have implications for an international framework of informatics competencies for all types of health care professionals including nurses.201728106584
clinical and genetic features of pkan patients in a tertiary centre in turkey.pantothenate kinase-associated neurodegeneration (pkan) is caused by mutations of the pantothenate kinase 2 (pank2) gene. the major clinical sign of pkan is dystonia and the eye-of-the-tiger pattern on the mri has been a clue for the diagnosis. we aim to discuss clinical and genetic findings of 22 pkan patients from 13 families.201728113101
first mass development of aedes albopictus (diptera: culicidae)-its surveillance and control in germany.the asian tiger mosquito aedes albopictus has undergone a dramatic expansion of its range in the last few decades. since its first detection in 2007 in germany at the motorway a5 coming from italy via switzerland to germany, it has been continuously introduced by vehicles, most probably from italy. after a hint from an alert gardener in an allotment garden area in freiburg, southwest germany, in 2015, a surveillance programme was started focusing on the garden area and adjacent areas as well as ...201728116530
malpighian tubules as novel targets for mosquito control.the malpighian tubules and hindgut are the renal excretory tissues of mosquitoes; they are essential to maintaining hemolymph water and solute homeostasis. moreover, they make important contributions to detoxifying metabolic wastes and xenobiotics in the hemolymph. we have focused on elucidating the molecular mechanisms of malpighian tubule function in adult female mosquitoes and developing chemical tools as prototypes for next-generation mosquitocides that would act via a novel mechanism of act ...201728125032
identification of mutant genes and introgressed tiger salamander dna in the laboratory axolotl, ambystoma mexicanum.the molecular genetic toolkit of the mexican axolotl, a classic model organism, has matured to the point where it is now possible to identify genes for mutant phenotypes. we used a positional cloning-candidate gene approach to identify molecular bases for two historic axolotl pigment phenotypes: white and albino. white (d/d) mutants have defects in pigment cell morphogenesis and differentiation, whereas albino (a/a) mutants lack melanin. we identified in white mutants a transcriptional defect in ...201728127056
genetic evidence for a worldwide chaotic dispersion pattern of the arbovirus vector, aedes albopictus.invasive species represent a global concern for their rapid spread and the possibility of infectious disease transmission. this is the case of the global invader aedes albopictus, the asian tiger mosquito. this species is a vector of medically important arboviruses, notably chikungunya (chikv), dengue (denv) and zika (zikv). the reconstruction of the complex colonization pattern of this mosquito has great potential for mitigating its spread and, consequently, disease risks.201728135274
dengue virus serotype 2 infection alters midgut and carcass gene expression in the asian tiger mosquito, aedes albopictus.the asian tiger mosquito, aedes albopictus is currently an important vector for dengue, chikungunya and zika virus, and its role in transmission of arthropod-borne viruses (arboviruses) may increase in the future due to its ability to colonize temperate regions. in contrast to aedes aegypti, the dominant vector of dengue, chikungunya and zika virus, genetic responses of ae. albopictus upon infection with an arbovirus are not well characterized. here we present a study of the changes in transcrip ...201728152011
effect of tiger-nut (cyperus esculentus) milk co-product on the surface and diffusional properties of a wheat-based matrix.the food processing industry generates huge volumes of waste and co-products which still contain valuable compounds. tiger-nut milk production generates large amounts of a co-product with a high insoluble fibre content, which is interesting as a bioactive component from a nutritional viewpoint. this co-product is formed by two different tissues in composition, particle size and colour terms, so two different flours were obtained from them. both flours were included in a wheat-based matrix at dif ...201728159295
new insights into the spermatogenesis of the black tiger prawn, penaeus monodon.this study reports a comprehensive description of penaeid spermatogenesis (penaeus monodon) by light and transmission electron microscopy. a conspicuous characteristic of spermatocytogenesis was a ring-like structure with high electron-density adjacent to the nucleus of a primary spermatocyte. during the spermiogenesis from stage i (sti) to stage vi spermatid (stvi), the formation of the acrosome and decondensation of the nucleus were the most notable morphological transformations. stis were sma ...201728164360
spatio-temporal interactions facilitate large carnivore sympatry across a resource gradient.species within a guild vary their use of time, space and resources, thereby enabling sympatry. as intra-guild competition intensifies, such behavioural adaptations may become prominent. we assessed mechanisms of facilitating sympatry among dhole (cuon alpinus), leopard (panthera pardus) and tiger (panthera tigris) in tropical forests of india using camera-trap surveys. we examined population-level temporal, spatial and spatio-temporal segregation among them across four reserves representing a gr ...201728179511
tiger beetles' (coleoptera: carabidae, cicindelinae) pupal stage: current state of knowledge and future perspectives.the tiger beetles (carabidae: cicindelinae) include about 2,822 species and 120 genera around the world. they are one of the most widely studied families of coleoptera. however, the knowledge about their immature stages is incipient and usually restricted to the larval stages. pupal characteristics have been among the most ignored aspects of tiger beetle biology. here we compile and update the current knowledge of tiger beetle pupae.201728187620
holding back the tiger: successful control program protects australia from aedes albopictus expansion.the asian tiger mosquito, aedes albopictus, is an important vector of dengue, chikungunya and zika viruses and is a highly invasive and aggressive biter. established populations of this species were first recognised in australia in 2005 when they were discovered on islands in the torres strait, between mainland australia and papua new guinea. a control program was implemented with the original goal of eliminating ae. albopictus from the torres strait. we describe the evolution of management stra ...201728192520
cataracts and strabismus associated with hand rearing using artificial milk formulas in bengal tiger (panthera tigris spp tigris) cubs.the aim of this investigation is to describe the potential contributing nutritional factors involved in the development of ophthalmic and dermatologic changes in four bengal tiger (panthera tigris spp tigris) cubs fed an artificial milk formula. the affected animals were compared with two other tiger cubs that had been nursed by their dam naturally. after the first clinical signs appeared, the tiger cubs underwent ophthalmic evaluation. severe symmetric generalized alopecia over the trunk, spari ...201728210544
functional nonredundancy of elephants in a disturbed tropical forest.conservation efforts are often motivated by the threat of global extinction. yet if conservationists had more information suggesting that the local extirpation of individual species could lead to undesirable ecological effects, conservationists might more frequently attempt to protect or restore such species across their range even if they are not in danger of global extinction. scientists have seldom measured or quantitatively predicted the functional consequences of species loss, even for larg ...201728218805
melanization inhibition protein of penaeus monodon acts as a negative regulator of the prophenoloxidase-activating system.melanization mediated by the prophenoloxidase-activating system (propo) is an important immune response in invertebrates. however, in addition to melanin, the propo system produces reactive intermediates that are not only harmful to the invading microbes but also to the host cells. thus, the propo system must be tightly regulated by several inhibitors. previously, a melanization inhibition protein from the black tiger shrimp penaeus monodon, pmmip, has been identified and preliminarily character ...201728232130
a tiger beetle's pursuit of prey depends on distance.tiger beetles pursue prey by adjusting their heading according to a time-delayed proportional control law that minimizes the error angle (haselsteiner et al 2014 j. r. soc. interface 11 20140216). this control law can be further interpreted in terms of mechanical actuation: to catch prey, tiger beetles exert a sideways force by biasing their tripod gait in proportion to the error angle measured half a stride earlier. the proportional gain was found to be nearly optimal in the sense that it minim ...201728233759
metal concentration in the tourist beaches of south durban: an industrial hub of south africa.south durban basin of south africa has witnessed tremendous urban, industrial expansion and mass tourism impacts exerting significant pressure over marine environments. 43 sediment samples from 7 different beaches (bluff beach; ansteys beach; brighton beach; cutting beach; isipingo beach; tiger rocks beach; amanzimtoti beach) were analyzed for acid leachable metals (alms) fe, mg, mn, cr, cu, mo, ni, co, pb, cd, zn and hg. the metal concentrations found in all the beaches were higher than the bac ...201728237076
modeling the population dynamics and community impacts of ambystoma tigrinum: a case study of phenotype plasticity.phenotypic plasticity is the ability of an organism to change its phenotype in response to changes in the environment. general mathematical descriptions of the phenomenon rely on an abstract measure of "viability" that, in this study, is instantiated in the case of the tiger salamander, ambystoma tigrinum. this organism has a point in its development when, upon maturing, it may take two very different forms. one is a terrestrial salamander (metamorph)that visits ponds to reproduce and eat, while ...201728237665
transcriptomic analysis of male black tiger shrimp (penaeus monodon) after polychaete feeding to enhance testicular maturation.to reveal molecular mechanism of how polychaetes enhanced reproductive maturation in the male black tiger shrimp (penaeus monodon), transcriptomic profiles of male reproductive organs (testes and vas deferens) between polychaete-fed and commercial pellet-fed male brooders were compared using cdna microarray. the overall profiles were distinguishingly different between the two feed groups as well as between testes and vas deferens. additionally, six of 11 differentially expressed gene identified ...201728246982
traditional chinese medicine bionic tiger bone powder for the treatment of ai-associated musculoskeletal symptoms.background. aromatase inhibitors (ais) are used for adjuvant therapy of breast cancer; however aimss (ai-associated musculoskeletal symptoms) can negatively affect quality of life and compliance. most patients in china moved to tcm (traditional chinese medicine) for help. tb (tiger bone) is used to treat bone disease, whose main ingredients are calcium and collagen. the objective of this study was to evaluate whether the tb prevented aimss in postmenopausal women with er/pr+ breast cancer. metho ...201728250792
molecular cloning, characterization and expression analysis of a novel pdrg1 gene from black tiger shrimp (penaeus monodon).p53 and dna damage-regulated gene 1 (pdrg1) is a novel gene which plays an important role in chaperone-mediated protein folding. in the present study, the full-length complementary dna (cdna) sequence of the pdrg1 gene from penaeus monodon (pmpdrg1) was cloned by the rapid amplification of cdna ends (race) method. the cdna of pmpdrg1 spans 1,613 bp, interrupted by only one short intron, and encodes a protein of 136 amino acids with calculated molecular weight of 15.49 kda. the temporal expressio ...201728257526
a new species of the genus <i>bertholdia</i> schaus, 1896 (lepidoptera: erebidae: arctiinae) from the neotropical region: <i>bertholdia</i> <i>zoenia</i> sp. n.a new species of bertholdia schaus is described: bertholdia zoenia sp. n., based on males and females from argentina and paraguay. this new species is closer to bertholdia myosticta hampson, 1901 from costa rica, irazú. bertholdia zoenia sp. n. can be recognized externally because its hyaline spot on forewing is the widest among all species of genus. also, the shape of this hyaline spot is like a right triangle with smooth outer margin, different from other species of genus, which have an irregu ...201728264269
the type-specimens of caraboidea beetles (coleoptera, adephaga) deposited in the collections of the i.i. schmalhausen institute of zoology, national academy of sciences of ukraine.a catalogue of type specimens of species and subspecies of caraboid beetles, tiger-beetles here treated as family cicindelidae, and ground-beetles (carabidae) of suborder adephaga deposited in the i.i. schmalhausen institute of zoology nas of ukraine is provided. for all type-specimens original photos of each specimen (with label) and label data are given in the original spelling (translated to english if the original label was in cyrillic alphabet). in some cases data concerning the current sta ...201728264278
effect of thermal processing on t cell reactivity of shellfish allergens - discordance with ige reactivity.crustacean allergy is a major cause of food-induced anaphylaxis. we showed previously that heating increases ige reactivity of crustacean allergens. here we investigate the effects of thermal processing of crustacean extracts on cellular immune reactivity. raw and cooked black tiger prawn, banana prawn, mud crab and blue swimmer crab extracts were prepared and ige reactivity assessed by elisa. mass spectrometry revealed a mix of several allergens in the raw mud crab extract but predominant heat- ...201728273149
bioaugmentation of the anaerobic digestion of food waste by dungs of herbivore, carnivore, and omnivore zoo animals.the potential improvement of biomethanation of food waste (fw) by adding dung of herbivore (giraffe, llama, koala), carnivore (tiger), and omnivore (sloth bear) animals to anaerobic sludge (ans) was investigated. adding 30% giraffe, sloth bear or koala dung to the ans inoculum yielded, respectively, a 11.17 (±4.51), 10.10 (±1.23), and 1.41 (±0.56)% higher biomethane production, as compared to the control (fw with solely ans). the highest biomethane production of 564.00 (±3.88) ml ch4/gvsadded ob ...201728274187
effectiveness of autodissemination stations containing pyriproxyfen in reducing immature aedes albopictus populations.aedes albopictus, the asian tiger mosquito, is an aggressive, highly anthropophilic, day-biting mosquito with an expanding geographic range. suppression of ae. albopictus is difficult because of the abundance and prevalence of larval habitats within peridomestic environments, particularly cryptic habitats such as corrugated extension spouts, fence post openings, discarded food containers, etc. because of the challenges of eliminating or treating larval habitats of this species, we tested an auto ...201728279191
the genetics of tiger pelage color variations. 201728281538
responsiveness of cats (felidae) to silver vine (actinidia polygama), tatarian honeysuckle (lonicera tatarica), valerian (valeriana officinalis) and catnip (nepeta cataria).olfactory stimulation is an often overlooked method of environmental enrichment for cats in captivity. the best known example of olfactory enrichment is the use of catnip, a plant that can cause an apparently euphoric reaction in domestic cats and most of the pantherinae. it has long been known that some domestic cats and most tigers do not respond to catnip. although many anecdotes exist of other plants with similar effects, data are lacking about the number of cats that respond to these plants ...201728302120
individual responses to population size structure: the role of size variation in controlling expression of a trophic polyphenism.we investigated how size structure affects development of alternative larval phenotypes in arizona tiger salamanders, ambystoma tigrinum nebulosum, by testing the hypothesis that population size structure per se is a significant component of an individual's environment. larvae of this subspecies exhibit one of two feeding phenotypes; typical larvae eat zooplankton and macroinvertebrates and cannibalistic larvae feed primarily on conspecifics. previous laboratory experiments showed that larval de ...199428307011
effects of different leaf traits on growth rates of insect herbivores on willows.we examined relative effects of traits of leaf quality of ten willow species (salix: salicaceae) on growth rates of five species of insect herbivores found in interior alaska (a willow sawfly, nematus calais; the tiger swallowtail butterfly, papilio canadensis; and three species of chrysomelid beetles, gonioctena occidentalis, calligrapha verrucosa, and chrysomela falsa). leaf traits examined were water content, toughness, total nitrogen contnet, pubescence, and presence or absence of phenolic g ...199428307038
patterns of induced and constitutive monoterpene production in conifer needles in relation to insect herbivory.studies were conducted to determine whether herbivore-induced synthesis of monoterpenes occurs in the needles of ponderosa pine (pinus ponderosa lawson), lodgepole pine (p. contorta douglas var. latifolia engelmann), white fir (abies concolor lindl. and gordon) and engelmann spruce [picea engelmanii (parry) engelm.]. in the needles of all species except engelmann spruce, simulated herbivory significantly induced the activity of monoterpene cyclases 4-8 days after wounding. in ponderosa pine, rea ...199828307902
effects of genetic structure of lupinus arboreus and previous herbivory on platyprepia virginalis caterpillars.two leaf-feeding caterpillars, western tussock moth (orgyia vetusta) and ranchman's tiger moth (platyprepia virginalis) are abundant on lupinus arboreus along the california coast. previous experiments and observations suggested that feeding caused by either of these two folivores could reduce the performance and possibly the abundance and distribution of the other species. previous common garden experiments also indicated that genetically determined characteristics of the host plants were impor ...199928308088
foraging under the risk of cannibalism leads to divergence in body size among tiger salamander larvae.populations of plants and animals are almost always made up of individuals of different sizes. in populations where cannibalism is common, this size variation can influence rates of mortality and growth and affect population regulation. size variation can be caused by a variety of mechanisms. one of these is due to size-specific responses to the threat of predation by potentially cannibalistic conspecifics. we investigated the role of anti-predator behavior in size structure development within s ...200028308182
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